SG11201807053TA - Compositions and methods for identifying rare cells - Google Patents
Compositions and methods for identifying rare cellsInfo
- Publication number
- SG11201807053TA SG11201807053TA SG11201807053TA SG11201807053TA SG11201807053TA SG 11201807053T A SG11201807053T A SG 11201807053TA SG 11201807053T A SG11201807053T A SG 11201807053TA SG 11201807053T A SG11201807053T A SG 11201807053TA SG 11201807053T A SG11201807053T A SG 11201807053TA
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- SG
- Singapore
- Prior art keywords
- cell
- interest
- international
- march
- methods
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 8
- 239000000203 mixture Substances 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 abstract 12
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 abstract 4
- 229920001477 hydrophilic polymer Polymers 0.000 abstract 3
- 230000000903 blocking effect Effects 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000003599 detergent Substances 0.000 abstract 2
- 102000008186 Collagen Human genes 0.000 abstract 1
- 108010035532 Collagen Proteins 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000005441 aurora Substances 0.000 abstract 1
- 229920001436 collagen Polymers 0.000 abstract 1
- 239000000834 fixative Substances 0.000 abstract 1
- 238000003384 imaging method Methods 0.000 abstract 1
- 238000010801 machine learning Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
- 239000003643 water by type Substances 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- G—PHYSICS
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- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/667—Neutral esters, e.g. sorbitan esters
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/046—Salts
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2065—Polyhydric alcohols
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/37—Polymers
- C11D3/3746—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C11D3/3769—(Co)polymerised monomers containing nitrogen, e.g. carbonamides, nitriles or amines
- C11D3/3776—Heterocyclic compounds, e.g. lactam
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/43—Solvents
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D9/00—Compositions of detergents based essentially on soap
- C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
- C11D9/22—Organic compounds, e.g. vitamins
- C11D9/225—Polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
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- G06T7/0014—Biomedical image inspection using an image reference approach
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/12—Soft surfaces, e.g. textile
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/40—Specific cleaning or washing processes
- C11D2111/44—Multi-step processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/10—DNA staining
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G06T2207/00—Indexing scheme for image analysis or image enhancement
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- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
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- G—PHYSICS
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- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biochemistry (AREA)
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- Immunology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Pathology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
ocentrifugea cell onto a slide T — — optionally Apply a first fixing buffer to the cell, the first fixing buffer including a hydrophilic polymer diluted in alcohol AppIya second fixing buffer to the cell, the second fixing buffer including a hydrophilic polymer, a detergent, and a fixative Silo S S13O W O 20 17 / 155 869 Al S150 buffer including a hydrophilic polymer, detergent, and hydrolyzed collagen ... ...... t optionally stain the cell Apply a blocking buffer to the cell, the blocking F10.1 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/155869 Al 14 September 2017 (14.09.2017) WIPO I PCT 111111111111110111011111111111010111110 01111111111111110111111110111111111110111111 (72) Inventor: BHAKDI, Sebastian, Chalirit, Pun- yaratabandhu; Lahnbergstrasse 17a, 35043 Marburg (DE). (74) Agent: SLOAT, Ashley; Aurora Consulting LLC, 1482 Woods And Waters Trl., Traverse City, MI 49696 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (51) International Patent Classification: C01F 7/04 (2006.01) Cl 2N 11/08 (2006.01) CO1G 37/10 (2006.01) C12Q 1/68 (2006.01) Cl 1D 3/12 (2006.01) G01N 21/75 (2006.01) C11D 9/22 (2006.01) (21) International Application Number: PCT/US2017/020905 (22) International Filing Date: 6 March 2017 (06.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/304,452 7 March 2016 (07.03.2016) US 62/313,366 25 March 2016 (25.03.2016) US 62/313,250 25 March 2016 (25.03.2016) US 62/430,542 6 December 2016 (06.12.2016) US (71) Applicant: X-ZELL INC. [US/US]; 640 West California Ave., Suite 210, Sunnyvale, CA 94086 (US). [Continued on next page] (54) Title: COMPOSITIONS AND METHODS FOR IDENTIFYING RARE CELLS (57) : Disclosed herein are compositions and methods of fix- 100 ing and staining rare cells. Farther, disclosed herein are methods of identifying circulating tumor cells (CTC). In some embodiments, the method includes: imaging a cell sample to identity a cell of interest; determining a first pixel intensity of a stained nuclear area; determin- ing a second pixel intensity of a background area; calculating a ploidy status of the cell of interest by subtracting the second pixel intensity from the first pixel intensify; and determining whether the cell of in- terest is a CTC based on the ploidy status. The method may be com- puter implemented, such, that the method uses a machine learning al- gorithm to identify a feature; process the feature to extract a parameter of interest; analyze the parameter of interest; and when the parameter of interest is greater than or less than a predetermined threshold, classi- fy the cell of interest as a CTC. 5140 optionally WO 2017/155869 Al MIDEDIMOMOIDEIR010131MMEIMMOVOIMIE TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662304452P | 2016-03-07 | 2016-03-07 | |
US201662313250P | 2016-03-25 | 2016-03-25 | |
US201662313366P | 2016-03-25 | 2016-03-25 | |
US201662430542P | 2016-12-06 | 2016-12-06 | |
PCT/US2017/020905 WO2017155869A1 (en) | 2016-03-07 | 2017-03-06 | Compositions and methods for identifying rare cells |
Publications (1)
Publication Number | Publication Date |
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SG11201807053TA true SG11201807053TA (en) | 2018-09-27 |
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ID=59790713
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SG10202009448VA SG10202009448VA (en) | 2016-03-07 | 2017-03-06 | Compositions and Methods for Identifying Rare Cells |
SG11201807053TA SG11201807053TA (en) | 2016-03-07 | 2017-03-06 | Compositions and methods for identifying rare cells |
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SG10202009448VA SG10202009448VA (en) | 2016-03-07 | 2017-03-06 | Compositions and Methods for Identifying Rare Cells |
Country Status (9)
Country | Link |
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US (2) | US11226271B2 (en) |
EP (2) | EP3426602B1 (en) |
JP (1) | JP7038062B2 (en) |
KR (1) | KR102392976B1 (en) |
CN (1) | CN108698846B (en) |
AU (1) | AU2017229088B2 (en) |
CA (2) | CA3220119A1 (en) |
SG (2) | SG10202009448VA (en) |
WO (1) | WO2017155869A1 (en) |
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US20200264076A1 (en) * | 2017-07-27 | 2020-08-20 | Cornell University | Fixation and retention of extracellular vesicles |
JP7076698B2 (en) * | 2017-11-17 | 2022-05-30 | 国立研究開発法人国立がん研究センター | Image analysis method, image analysis device, program, learned deep learning algorithm manufacturing method and learned deep learning algorithm |
CN108254547A (en) * | 2018-01-03 | 2018-07-06 | 安徽科技学院 | A kind of fluorescence immunoassay group method of body early embryo tissue |
CN108226470A (en) * | 2018-01-10 | 2018-06-29 | 安徽科技学院 | A kind of method that embryo's integrality is kept in body early embryo histogenic immunity group |
JP2019195304A (en) | 2018-05-10 | 2019-11-14 | 学校法人順天堂 | Image analysis method, device, computer program, and generation method of deep learning algorithm |
EP3891297A4 (en) * | 2018-12-03 | 2022-09-07 | California Institute of Technology | In situ readout of dna barcodes |
JP7213487B2 (en) * | 2019-03-07 | 2023-01-27 | 住友ゴム工業株式会社 | Cancer cell culture method |
JP7381003B2 (en) * | 2019-04-26 | 2023-11-15 | 学校法人順天堂 | METHODS, APPARATUS AND COMPUTER PROGRAMS TO ASSIST DISEASE ANALYSIS AND METHODS, APPARATUS AND PROGRAMS FOR TRAINING COMPUTER ALGORITHM |
EP3977402A1 (en) | 2019-05-28 | 2022-04-06 | PAIGE.AI, Inc. | Systems and methods for processing images to prepare slides for processed images for digital pathology |
JP7475848B2 (en) | 2019-11-29 | 2024-04-30 | シスメックス株式会社 | CELL ANALYSIS METHOD, CELL ANALYSIS APPARATUS, CELL ANALYSIS SYSTEM, CELL ANALYSIS PROGRAM, AND GENERATION METHOD, GENERATION APPARATUS, AND GENERATION PROGRAM FOR TRAINED ARTIFICIAL INTELLIGENCE ALGORITHM |
WO2021132576A1 (en) * | 2019-12-25 | 2021-07-01 | 有限会社マイテック | Autofluorescence-based liquid biopsy method targeting nucleosomes fragmented by apoptosis |
CN111461243B (en) * | 2020-04-08 | 2023-06-20 | 中国医学科学院肿瘤医院 | Classification method, classification device, electronic equipment and computer-readable storage medium |
CN111739010B (en) | 2020-06-23 | 2022-06-24 | 珠海圣美生物诊断技术有限公司 | Method and device for detecting abnormal circulating cells |
TWI818278B (en) * | 2020-11-20 | 2023-10-11 | 日商米特奇有限公司 | Autofluorescent liquid biopsy method targeting nucleosome fragmented by apoptosis |
CN115541579B (en) * | 2022-10-09 | 2023-07-07 | 广州医科大学附属第一医院(广州呼吸中心) | Method and device for analyzing Ki-67 positive tumor cell proportion in tumor section for non-diagnosis and treatment purposes |
CN115690785B (en) * | 2022-12-08 | 2023-06-06 | 广东美赛尔细胞生物科技有限公司 | Temperature control method and system applied to freeze-dried cell storage |
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KR102392976B1 (en) | 2022-04-29 |
WO2017155869A1 (en) | 2017-09-14 |
JP2019513228A (en) | 2019-05-23 |
JP7038062B2 (en) | 2022-03-17 |
EP3426602A1 (en) | 2019-01-16 |
CN108698846A (en) | 2018-10-23 |
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