SG11201806557VA - Differentiation induction from human pluripotent stem cells into hypothalamic neurons - Google Patents

Differentiation induction from human pluripotent stem cells into hypothalamic neurons

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Publication number
SG11201806557VA
SG11201806557VA SG11201806557VA SG11201806557VA SG11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA
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Singapore
Prior art keywords
pluripotent stem
human pluripotent
stem cells
suspension
substance
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SG11201806557VA
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Hidetaka Suga
Koichiro Ogawa
Takatoshi Kasai
Hiroshi Arima
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Univ Nagoya Nat Univ Corp
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Publication date
Application filed by Univ Nagoya Nat Univ Corp filed Critical Univ Nagoya Nat Univ Corp
Publication of SG11201806557VA publication Critical patent/SG11201806557VA/en

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Abstract

DIFFERENTIATION INDUCTION FROM HUMAN PLURIPOTENT STEM CELLS INTO HYPOTHALAMIC NEURONS 5 There is provided a method for efficient differentiation induction from human pluripotent stem cells into hypothalamic neurons. Also, provided is a method for constructing, from human pluripotent stem cells, a cellular structure in which hypothalamic tissue and pituitary tissue are integrated. A cellular structure including hypothalamic tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem 10 cells in suspension in a medium containing a low concentration of a bone morphogenetic protein signal transduction pathway activating substance and a low concentration of a substance acting on the Shh signaling pathway; and further culturing the cell aggregate obtained in the step in suspension in a medium containing a low concentration of a substance acting on the Shh signaling pathway. Also, a cellular structure including hypothalamic tissue 15 and pituitary tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signaling pathway; further culturing the cell aggregate formed in the step in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating 20 substance and a substance acting on the Shh signaling pathway; and culturing the cell aggregate obtained in the step in suspension in a medium suitable for simultaneous induction of pituitary and hypothalamus. [Figure 14] 25
SG11201806557VA 2016-01-22 2017-01-18 Differentiation induction from human pluripotent stem cells into hypothalamic neurons SG11201806557VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016010940 2016-01-22
PCT/JP2017/001544 WO2017126551A1 (en) 2016-01-22 2017-01-18 Differentiation induction from human pluripotent stem cells into hypothalamic neurons

Publications (1)

Publication Number Publication Date
SG11201806557VA true SG11201806557VA (en) 2018-08-30

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Country Status (11)

Country Link
US (1) US20190010452A1 (en)
EP (1) EP3406709A4 (en)
JP (2) JP7023496B2 (en)
KR (1) KR20180101467A (en)
CN (1) CN108495929B (en)
AU (1) AU2017209742B2 (en)
CA (1) CA3013553A1 (en)
IL (1) IL260380B2 (en)
MY (1) MY194128A (en)
SG (1) SG11201806557VA (en)
WO (1) WO2017126551A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202004829WA (en) * 2017-11-24 2020-06-29 Sumitomo Chemical Co Method for producing cell mass including pituitary tissue, and cell mass thereof
EP4063496A1 (en) 2019-11-20 2022-09-28 Sumitomo Pharma Co., Ltd. Method for freezing neural cells
EP4063495A4 (en) 2019-11-20 2023-12-27 Sumitomo Pharma Co., Ltd. Method for freezing cell aggregates
CN113046306B (en) * 2021-03-12 2022-10-18 广东东阳光药业有限公司 Culture method of pluripotent stem cells
TW202330905A (en) * 2021-09-30 2023-08-01 日商住友製藥股份有限公司 Cell aggregate including pituitary hormone-producing cells and method for producing same
WO2023054396A1 (en) * 2021-09-30 2023-04-06 住友化学株式会社 Method for producing cell mass including pituitary tissue and cell mass

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* Cited by examiner, † Cited by third party
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KR100763477B1 (en) * 2003-03-12 2007-10-04 리라이언스 라이프 사이언시스 프라이빗. 리미티드 Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells
EP2774983B1 (en) * 2011-10-31 2019-05-15 Riken Method for culturing stem cell
AU2014236150B2 (en) * 2013-03-14 2019-10-31 The Regents Of The University Of California In vitro production of medial ganglionic eminence precursor cells
EP3072960B1 (en) * 2013-11-22 2019-03-27 Riken Method for manufacturing telencephalon or progenitor tissue thereof
IL250258B (en) * 2014-07-25 2022-07-01 Riken Method for producing adenohypophysis or precursor tissue thereof

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JPWO2017126551A1 (en) 2018-11-22
JP2022062165A (en) 2022-04-19
MY194128A (en) 2022-11-14
JP7023496B2 (en) 2022-02-22
KR20180101467A (en) 2018-09-12
CN108495929A (en) 2018-09-04
IL260380B1 (en) 2023-05-01
AU2017209742B2 (en) 2022-12-01
EP3406709A4 (en) 2019-08-28
AU2017209742A1 (en) 2018-08-23
IL260380B2 (en) 2023-09-01
US20190010452A1 (en) 2019-01-10
CN108495929B (en) 2023-04-18
CA3013553A1 (en) 2017-07-27
JP7465569B2 (en) 2024-04-11
EP3406709A1 (en) 2018-11-28
WO2017126551A1 (en) 2017-07-27
IL260380A (en) 2018-08-30

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