SG11201803918VA - Production of steviol glycosides in recombinant hosts - Google Patents

Production of steviol glycosides in recombinant hosts

Info

Publication number
SG11201803918VA
SG11201803918VA SG11201803918VA SG11201803918VA SG11201803918VA SG 11201803918V A SG11201803918V A SG 11201803918VA SG 11201803918V A SG11201803918V A SG 11201803918VA SG 11201803918V A SG11201803918V A SG 11201803918VA SG 11201803918V A SG11201803918V A SG 11201803918VA
Authority
SG
Singapore
Prior art keywords
international
pct
steviol glycosides
duggingerstrasse
reinach
Prior art date
Application number
SG11201803918VA
Inventor
Jane Dannow Dyekjaer
Jens Houghton-Larsen
Veronique Douchin
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of SG11201803918VA publication Critical patent/SG11201803918VA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau 111111111111110111010101111101011111001110111111110111110101111111111111110111111 (43) International Publication Date 15 June 2017 (15.06.2017) (10) International Publication Number WO 2017/098017 Al WIPO I PCT (51) International Patent Classification: C12N 15/81 (2006.01) C12N 15/82 C07K 14/395 (2006.01) C12P 15/00 C12N 9/10 (2006.01) A23L 27/30 C12N 9/90 (2006.01) (2006.01) (2006.01) (2016.01) (21) International Application Number: PCT/EP2016/080516 (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/265,895 10 December 2015 (10.12.2015) US (71) Applicant: EVOLVA SA [CH/CH]; Duggingerstrasse 23, 4153 Reinach (CH). (72) Inventors: DYEKJAER, Jane Dannow; Duggingerstrasse 23, 4153 Reinach (CH). HOUGHTON-LARSEN, Jens; Groendalsvej 13, 3460 Birkeroed (DK). DOUCHIN, Ver- onique; Aurikelvej 2, 4th, 2000 Frederiksberg (DK). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). 9 December 2016 (09.12.2016) English English (74) Agent: SMAGGASGALE, Gillian Helen; 138 Fetter Lane, London EC4A 1BT (GB). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) W O 20 17 /0980 17 Al (54) Title: PRODUCTION OF STEVIOL GLYCOSIDES IN RECOMBINANT HOSTS (57) : The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glyc- oside precursors.
SG11201803918VA 2015-12-10 2016-12-09 Production of steviol glycosides in recombinant hosts SG11201803918VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562265895P 2015-12-10 2015-12-10
PCT/EP2016/080516 WO2017098017A1 (en) 2015-12-10 2016-12-09 Production of steviol glycosides in recombinant hosts

Publications (1)

Publication Number Publication Date
SG11201803918VA true SG11201803918VA (en) 2018-06-28

Family

ID=57714564

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201803918VA SG11201803918VA (en) 2015-12-10 2016-12-09 Production of steviol glycosides in recombinant hosts

Country Status (9)

Country Link
US (2) US20190062796A1 (en)
EP (1) EP3387136B1 (en)
JP (1) JP2019503670A (en)
CN (1) CN108473995B (en)
AU (1) AU2016367317A1 (en)
BR (1) BR112018011611A2 (en)
CA (1) CA3007726A1 (en)
SG (1) SG11201803918VA (en)
WO (1) WO2017098017A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201808856TA (en) 2016-04-13 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
PT3497222T (en) 2016-08-12 2022-02-09 Amyris Inc Udp-dependent glycosyltransferase for high efficiency production of rebaudiosides
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3878967A3 (en) 2017-02-03 2021-12-08 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
EP3644758A4 (en) * 2017-06-30 2021-03-31 Conagen Inc. Hydrolysis of steviol glycosides by beta-glucosidase
CN111868252A (en) * 2018-03-12 2020-10-30 科纳根公司 Biosynthetic production of steviol glycosides rebaudioside J and rebaudioside N
JP7337068B2 (en) * 2018-07-31 2023-09-01 サントリーホールディングス株式会社 Stevia plant with high sweetener content and screening method thereof
CN110684813A (en) * 2019-09-18 2020-01-14 江苏施宇甜生物科技有限公司 Method for producing steviol glycosides in recombinant hosts
CN117015611A (en) * 2021-03-01 2023-11-07 嘉吉公司 Xylosylated steviol glycosides and enzymatic process for production

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011140329A1 (en) * 2010-05-06 2011-11-10 Ceres, Inc. Transgenic plants having increased biomass
BR122021005283B1 (en) * 2010-06-02 2022-02-22 Evolva, Inc RECOMBINANT HOST COMPRISING RECOMBINANT GENES FOR PRODUCTION OF STEVIOL OR STEVIOL GLYCOSIDE, METHOD FOR PRODUCING STEVIOL, STEVIOL GLYCOSIDE OR STEVIOL GLYCOSIDE COMPOSITION AND METHOD FOR SYNTHESISTING STEVIOL OR STEVIOL GLYCOSIDE
KR20200057799A (en) * 2011-08-08 2020-05-26 에볼바 에스아 Recombinant production of steviol glycosides
US9611498B2 (en) * 2012-09-29 2017-04-04 Shanghai Institutes For Biological Sciences, Chinese Academy Of Sciences Method for producing stevioside compounds by microorganism
CA2899276C (en) * 2013-02-06 2022-11-01 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
EP3004365A1 (en) * 2013-05-31 2016-04-13 DSM IP Assets B.V. Extracellular diterpene production
WO2015011209A1 (en) * 2013-07-23 2015-01-29 Dsm Ip Assets B.V. Diterpene production in yarrowia
US20160185813A1 (en) * 2013-07-31 2016-06-30 Dsm Ip Assets B.V. Recovery of steviol glycosides
US9957539B2 (en) * 2013-08-02 2018-05-01 Suntory Holdings Limited Method for using hexenol glycosyl transferase
CN106572688B (en) * 2014-08-11 2022-01-25 埃沃尔瓦公司 Production of steviol glycosides in recombinant hosts

Also Published As

Publication number Publication date
EP3387136A1 (en) 2018-10-17
CN108473995B (en) 2022-10-18
JP2019503670A (en) 2019-02-14
EP3387136B1 (en) 2022-01-05
CN108473995A (en) 2018-08-31
AU2016367317A1 (en) 2018-05-24
US20190062796A1 (en) 2019-02-28
BR112018011611A2 (en) 2018-11-27
CA3007726A1 (en) 2017-06-15
US20220195477A1 (en) 2022-06-23
WO2017098017A1 (en) 2017-06-15

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