SG11201703472YA - Transposase compositions for reduction of insertion bias - Google Patents

Transposase compositions for reduction of insertion bias

Info

Publication number
SG11201703472YA
SG11201703472YA SG11201703472YA SG11201703472YA SG11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA SG 11201703472Y A SG11201703472Y A SG 11201703472YA
Authority
SG
Singapore
Prior art keywords
reduction
insertion bias
transposase
compositions
transposase compositions
Prior art date
Application number
SG11201703472YA
Other languages
English (en)
Inventor
Christian Gloeckner
Amirali Kia
Molly He
Trina Faye Osothprarop
Frank J Steemers
Kevin L Gunderson
Sasan Amini
Jerome Jendrisak
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG11201703472YA publication Critical patent/SG11201703472YA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Non-Portable Lighting Devices Or Systems Thereof (AREA)
  • Endoscopes (AREA)
  • Fittings On The Vehicle Exterior For Carrying Loads, And Devices For Holding Or Mounting Articles (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Mechanical Coupling Of Light Guides (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
  • Fuel-Injection Apparatus (AREA)
SG11201703472YA 2014-11-05 2015-11-05 Transposase compositions for reduction of insertion bias SG11201703472YA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462075713P 2014-11-05 2014-11-05
US201562242935P 2015-10-16 2015-10-16
PCT/US2015/059194 WO2016073690A1 (en) 2014-11-05 2015-11-05 Transposase compositions for reduction of insertion bias

Publications (1)

Publication Number Publication Date
SG11201703472YA true SG11201703472YA (en) 2017-05-30

Family

ID=54697644

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201703472YA SG11201703472YA (en) 2014-11-05 2015-11-05 Transposase compositions for reduction of insertion bias

Country Status (10)

Country Link
US (2) US10815478B2 (es)
EP (3) EP3215618B1 (es)
CN (1) CN107532164A (es)
AU (2) AU2015343034B2 (es)
CA (1) CA2965995A1 (es)
DK (2) DK3628732T3 (es)
ES (1) ES2947757T3 (es)
FI (1) FI3628732T3 (es)
SG (1) SG11201703472YA (es)
WO (1) WO2016073690A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2947757T3 (es) * 2014-11-05 2023-08-18 Illumina Inc Composiciones de transposasas para reducir el sesgo de inserción
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
CA3105722C (en) 2015-12-07 2022-10-11 Zymergen Inc. Microbial strain improvement by a htp genomic engineering platform
BR112018011503A2 (pt) 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
WO2018005655A2 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
RU2019106038A (ru) * 2016-08-10 2020-09-17 Президент Энд Фэллоуз Оф Харвард Коллидж Способы de novo сборки баркодированных фрагментов геномной днк
KR102551666B1 (ko) * 2016-12-29 2023-07-04 일루미나, 인코포레이티드 세포 구획 내의 생체분자에 대한 직교 접근 및 그의 태그부착을 위한 분석 시스템
EP3625351A1 (en) 2017-05-19 2020-03-25 Zymergen Inc. Genomic engineering of biosynthetic pathways leading to increased nadph
US20200370058A1 (en) 2017-06-06 2020-11-26 Zymergen Inc. A htp genomic engineering platform for improving escherichia coli
CN110914425B (zh) 2017-06-06 2024-06-25 齐默尔根公司 用于改良刺糖多孢菌的高通量(htp)基因组工程改造平台
EP3635112A2 (en) 2017-06-06 2020-04-15 Zymergen, Inc. A htp genomic engineering platform for improving fungal strains
EP3635111A1 (en) 2017-06-06 2020-04-15 Zymergen, Inc. High throughput transposon mutagenesis
EP3768844A4 (en) 2018-03-20 2021-12-15 Zymergen Inc. HTP PLATFORM FOR GENETIC ENGINEERING OF OVARIAN CELLS FROM CHINESE HAMSTER
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
EP3918088B1 (en) 2019-01-29 2024-03-13 MGI Tech Co., Ltd. High coverage stlfr
AU2020312787A1 (en) * 2019-07-12 2021-06-17 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
CN115349128A (zh) 2020-02-13 2022-11-15 齐默尔根公司 宏基因组文库和天然产物发现平台
WO2024003332A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
FI103809B1 (fi) * 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
CN1181852C (zh) * 2002-05-21 2004-12-29 崔行善 一种治疗膀胱癌的药物
FR2900935A1 (fr) * 2006-05-15 2007-11-16 Centre Nat Rech Scient Systemes de transposition recombinants hyperactifs derives du transposon mos-1
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2787565B1 (en) 2008-10-24 2015-09-16 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2012061832A1 (en) * 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
US9005935B2 (en) * 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
WO2013085918A1 (en) 2011-12-05 2013-06-13 The Regents Of The University Of California Methods and compostions for generating polynucleic acid fragments
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20160208323A1 (en) * 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
WO2015089339A2 (en) * 2013-12-11 2015-06-18 Changping Shi Compositions, methods and kits for dna fragmentation and tagmentation
WO2015160895A2 (en) * 2014-04-15 2015-10-22 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased dna input tolerance
ES2947757T3 (es) * 2014-11-05 2023-08-18 Illumina Inc Composiciones de transposasas para reducir el sesgo de inserción

Also Published As

Publication number Publication date
EP3215618B1 (en) 2019-07-24
AU2015343034B2 (en) 2021-12-16
EP4239067A3 (en) 2023-10-25
US20180016571A1 (en) 2018-01-18
US20210047635A1 (en) 2021-02-18
DK3215618T3 (da) 2019-10-14
AU2015343034A1 (en) 2017-05-18
AU2015343034A9 (en) 2019-08-01
CA2965995A1 (en) 2016-05-12
EP4239067A2 (en) 2023-09-06
WO2016073690A1 (en) 2016-05-12
AU2022201248A1 (en) 2022-03-17
EP3628732A1 (en) 2020-04-01
DK3628732T3 (da) 2023-05-30
CN107532164A (zh) 2018-01-02
WO2016073690A9 (en) 2017-09-14
US10815478B2 (en) 2020-10-27
EP3215618A1 (en) 2017-09-13
ES2947757T3 (es) 2023-08-18
EP3628732B1 (en) 2023-05-03
FI3628732T3 (fi) 2023-05-31

Similar Documents

Publication Publication Date Title
SG11201703472YA (en) Transposase compositions for reduction of insertion bias
IL251584A0 (en) Dihydropyrrolopyridines ror-gamma inhibitors
ZA201700304B (en) Spirocycloheptanes as inhibitors of rock
IL246902A0 (en) Dihydropyrrolopyridine ror–gamma inhibitors
IL251420A0 (en) Triazolopyrazinones as pde1 inhibitors
EP3151846A4 (en) Methods and compositions for nuclease design
AP2016009496A0 (en) Substituted spirocydic inhibitors of autotaxin
HUE055357T2 (hu) Vegyületek kompozíciói és azok alkalmazásai
HUE051713T2 (hu) Szelenoorganikus vegyületeket tartalmazó készítmények és ezek alkalmazása
GB201521059D0 (en) Inhibitors of metallo-beta-lactamases
HK1246193A1 (zh) 用於改善認知的方法和組合物
PT3113774T (pt) Composições de grapiprant e métodos de utilização das mesmas
HK1232214A1 (zh) 作為 抑制劑的六氫呋喃並吡咯
GB201418410D0 (en) use of compositions of matter
GB201412359D0 (en) Composition of objects
GB201412089D0 (en) Composition of objects
GB201502834D0 (en) Composition of objects
GB201417347D0 (en) Prodrugs of delanzomib
GB201417348D0 (en) Prodrugs of lxazomib