SG11201607114VA - Expression constructs and methods for selecting host cells expressing polypeptides - Google Patents

Expression constructs and methods for selecting host cells expressing polypeptides

Info

Publication number
SG11201607114VA
SG11201607114VA SG11201607114VA SG11201607114VA SG11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA SG 11201607114V A SG11201607114V A SG 11201607114VA
Authority
SG
Singapore
Prior art keywords
methods
host cells
cells expressing
expression constructs
expressing polypeptides
Prior art date
Application number
SG11201607114VA
Inventor
Christel Aebischer-Gumy
Pierre Moretti
Martin Bertschinger
Original Assignee
Glenmark Pharmaceuticals Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glenmark Pharmaceuticals Sa filed Critical Glenmark Pharmaceuticals Sa
Publication of SG11201607114VA publication Critical patent/SG11201607114VA/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/30Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622Single chain antibody (scFv)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/42Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/50Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11201607114VA 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides SG11201607114VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14157324 2014-02-28
PCT/EP2015/054331 WO2015128509A1 (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides

Publications (1)

Publication Number Publication Date
SG11201607114VA true SG11201607114VA (en) 2016-09-29

Family

ID=50184819

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201607114VA SG11201607114VA (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides
SG10201913396RA SG10201913396RA (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10201913396RA SG10201913396RA (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides

Country Status (15)

Country Link
US (2) US20170350886A1 (en)
EP (1) EP3110950B1 (en)
JP (1) JP7008406B2 (en)
KR (1) KR20160135738A (en)
CN (1) CN106255752A (en)
AU (1) AU2015222065B2 (en)
BR (1) BR112016019832A2 (en)
CA (1) CA2940880C (en)
EA (1) EA035266B1 (en)
IL (1) IL247491A0 (en)
MA (1) MA39502A (en)
MX (1) MX2016011194A (en)
NZ (1) NZ723624A (en)
SG (2) SG11201607114VA (en)
WO (1) WO2015128509A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2965292T3 (en) * 2015-07-09 2024-04-12 Inst Nat Sante Rech Med Lentiviral vector expressing membrane-anchored or secreted antibody
CA3026778A1 (en) * 2016-06-07 2017-12-14 Max-Delbruck-Centrum Fur Molekulare Medizin In Der Helmholtz-Gemeinschaft Chimeric antigen receptor and car-t cells that bind bcma
JP2020518622A (en) * 2017-05-02 2020-06-25 アリゲーター・バイオサイエンス・アーベー Bispecific antibodies to OX40 and CTLA-4
WO2019169071A1 (en) * 2018-03-01 2019-09-06 University Of Washington Transmembrane polypeptides
WO2020005819A1 (en) * 2018-06-25 2020-01-02 University Of Washington De novo design of potent and selective interleukin mimetics
WO2020260431A1 (en) 2019-06-28 2020-12-30 F. Hoffmann-La Roche Ag Method for the production of an antibody
KR20240038000A (en) 2021-07-08 2024-03-22 메딕 라이프 사이언시스 인코포레이티드 High-throughput screening system for identification of novel drugs and drug targets
CN113881701B (en) * 2021-09-08 2024-04-19 山东大学 Positive transfected cell affinity sorting method and kit

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2007251766B2 (en) * 2006-05-17 2013-06-20 F. Hoffmann-La Roche Ag Polypeptide producing cells
WO2009059235A2 (en) * 2007-11-01 2009-05-07 Facet Biotech Corporation Immunoglobulin display vectors
US8969539B2 (en) * 2008-06-11 2015-03-03 Jn Biosciences Llc Expression vectors
WO2014126254A1 (en) * 2013-02-18 2014-08-21 協和発酵キリン株式会社 Membrane-binding protein expression vector

Also Published As

Publication number Publication date
CA2940880C (en) 2023-09-26
EP3110950A1 (en) 2017-01-04
EA035266B1 (en) 2020-05-22
US20170350886A1 (en) 2017-12-07
MX2016011194A (en) 2017-04-06
AU2015222065A1 (en) 2016-09-15
CA2940880A1 (en) 2015-09-03
EA201691548A1 (en) 2017-02-28
JP2017508459A (en) 2017-03-30
SG10201913396RA (en) 2020-03-30
JP7008406B2 (en) 2022-01-25
EP3110950B1 (en) 2022-09-21
CN106255752A (en) 2016-12-21
AU2015222065B2 (en) 2021-05-20
NZ723624A (en) 2022-07-29
IL247491A0 (en) 2016-11-30
WO2015128509A1 (en) 2015-09-03
KR20160135738A (en) 2016-11-28
MA39502A (en) 2015-09-03
US20190056396A1 (en) 2019-02-21
BR112016019832A2 (en) 2017-10-17

Similar Documents

Publication Publication Date Title
IL282168A (en) Factor viii chimeric proteins and uses thereof
IL248361A0 (en) Recombinant host cell for expressing proteins of interest
IL249373A0 (en) Formulated receptor polypeptides and related methods
IL247491A0 (en) Expression constructs and methods for selecting host cells expressing polypeptides
LT3212660T (en) Mammalian cells expressing cytomegalovirus antigens
HK1248272A1 (en) Host cell protein modification
LT3131577T (en) Modified host cells and uses thereof
EP3107996A4 (en) Tscm cells and methods for use
IL249368B (en) Map44 polypeptides and constructs based on natural antibodies and uses thereof
IL246642A0 (en) Materials and methods for measuring pld-1 protein levels
SG11201606501PA (en) Nanobody-fluorescent protein fusion
EP3140275A4 (en) Bh4 antagonists and methods related thereto
GB201419897D0 (en) Host cell
GB201403912D0 (en) Proteins
GB201403913D0 (en) Proteins
GB201403914D0 (en) Proteins