SG11201606934SA - Improved reprogramming methods and cell culture platforms - Google Patents

Improved reprogramming methods and cell culture platforms

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Publication number
SG11201606934SA
SG11201606934SA SG11201606934SA SG11201606934SA SG11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA SG 11201606934S A SG11201606934S A SG 11201606934SA
Authority
SG
Singapore
Prior art keywords
cell culture
reprogramming methods
culture platforms
improved reprogramming
improved
Prior art date
Application number
SG11201606934SA
Inventor
Bahram Valamehr
Peter Flynn
Ramzey Abujarour
Megan Robinson
Original Assignee
Fate Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fate Therapeutics Inc filed Critical Fate Therapeutics Inc
Publication of SG11201606934SA publication Critical patent/SG11201606934SA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/724—Glycosyltransferases (EC 2.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
SG11201606934SA 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms SG11201606934SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461947979P 2014-03-04 2014-03-04
PCT/US2015/018801 WO2015134652A1 (en) 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms

Publications (1)

Publication Number Publication Date
SG11201606934SA true SG11201606934SA (en) 2016-09-29

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SG11201606934SA SG11201606934SA (en) 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms
SG10201807292YA SG10201807292YA (en) 2014-03-04 2015-03-04 Improved reprogramming methods and cell culture platforms

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Country Status (11)

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US (2) US11268069B2 (en)
EP (3) EP3805369A1 (en)
JP (6) JP6722108B2 (en)
KR (3) KR102340553B1 (en)
CN (1) CN106414721A (en)
AU (2) AU2015227184B2 (en)
CA (1) CA2941004A1 (en)
ES (1) ES2966757T3 (en)
PT (1) PT3114214T (en)
SG (2) SG11201606934SA (en)
WO (1) WO2015134652A1 (en)

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SG11201606934SA (en) * 2014-03-04 2016-09-29 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
SG11201706041XA (en) 2015-01-26 2017-08-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
BR112018001232A2 (en) 2015-07-21 2018-09-25 The Children's Medical Center Corporation pd-l1 expressing hematopoietic stem cells and uses
CN117737124A (en) 2015-10-16 2024-03-22 菲特治疗公司 Platform for inducing and maintaining ground state pluripotency
AU2016341982B2 (en) * 2015-10-21 2022-07-07 Indiana University Research And Technology Corporation Methods of generating human inner ear sensory epithelia and sensory neurons
AU2016349504B2 (en) 2015-11-04 2023-02-09 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
US10858628B2 (en) 2015-11-04 2020-12-08 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
CA3010764A1 (en) * 2016-01-12 2017-07-20 Lonza Walkersville, Inc. Methods and vectors to produce vector free induced pluripotent stem cells
CN110114470A (en) * 2016-12-27 2019-08-09 住友化学株式会社 The preparation method of the multipotent stem cells of the evaluation method and selection method and induction of the multipotent stem cells of induction
CN106754729B (en) * 2016-12-31 2020-03-17 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 Method for preparing induced pluripotent stem cells by using Xist Tale inhibitory transcription factor
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation Treatment of type 1 diabetes and autoimmune diseases or disorders
CA3078734A1 (en) * 2017-10-11 2019-04-18 Fate Therapeutics, Inc. Cellular reprogramming using temporal and transient plasmid vector expression system
CN111542594A (en) 2017-12-22 2020-08-14 菲特治疗公司 Enhanced immune effector cells and uses thereof
US20200339958A1 (en) * 2017-12-28 2020-10-29 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glad Generation of induced pluripotent cells by crispr activation
EP3768827A1 (en) * 2018-03-22 2021-01-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for reprogramming somatic cells
CN109679918A (en) * 2018-12-25 2019-04-26 合肥中科干细胞再生医学有限公司 A kind of preparation method of convenient and fast people's inductive pluripotent stem cells
WO2020203712A1 (en) 2019-03-29 2020-10-08 富士フイルム株式会社 Method for producing pluripotent stem cells capable of differentiating into specific cells, and application thereof
CN110564673A (en) * 2019-09-26 2019-12-13 广东工业大学 Stem cell culture solution and application thereof
WO2021116362A1 (en) * 2019-12-11 2021-06-17 Repairon Gmbh Expansion of stem cells in suspension in a bioreactor
AU2021296296A1 (en) * 2020-06-22 2023-01-19 Life Technologies Corporation Methods and compositions for cultivating pluripotent cell suspensions
WO2022072883A1 (en) * 2020-10-02 2022-04-07 Fate Therapeutics, Inc. Improved reprogramming, maintenance and preservation for induced pluripotent stem cells
US20230302044A1 (en) * 2022-03-28 2023-09-28 Steve Reilly Composition to increase cellularlongevity

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