SG11201602629RA - Microfluidic devices having isolation pens and methods of testing biological micro-objects with same - Google Patents

Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Info

Publication number
SG11201602629RA
SG11201602629RA SG11201602629RA SG11201602629RA SG11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA SG 11201602629R A SG11201602629R A SG 11201602629RA
Authority
SG
Singapore
Prior art keywords
objects
methods
same
microfluidic devices
biological micro
Prior art date
Application number
SG11201602629RA
Inventor
Eric D Hobbs
Mark P White
J Tanner Nevill
Daniele Malleo
Steven W Short
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52993522&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG11201602629R(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11201602629RA publication Critical patent/SG11201602629RA/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201602629RA 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same SG11201602629RA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361996969P 2013-10-22 2013-10-22
US201462058658P 2014-10-01 2014-10-01
PCT/US2014/061837 WO2015061497A1 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US14/520,568 US10010882B2 (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Publications (1)

Publication Number Publication Date
SG11201602629RA true SG11201602629RA (en) 2016-05-30

Family

ID=52993522

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201602629RA SG11201602629RA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
SG10202006525PA SG10202006525PA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202006525PA SG10202006525PA (en) 2013-10-22 2014-10-22 Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Country Status (9)

Country Link
US (4) US10010882B2 (en)
EP (3) EP3473700B1 (en)
JP (4) JP6557658B2 (en)
KR (4) KR102358356B1 (en)
AU (3) AU2014340089B2 (en)
CA (2) CA3095333C (en)
IL (3) IL297654A (en)
SG (2) SG11201602629RA (en)
WO (1) WO2015061497A1 (en)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013176767A1 (en) 2012-05-25 2013-11-28 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
AU2014340089B2 (en) 2013-10-22 2019-09-26 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
GB201320146D0 (en) 2013-11-14 2014-01-01 Cambridge Entpr Ltd Fluidic separation and detection
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US11754563B2 (en) * 2014-11-20 2023-09-12 Global Life Sciences Solutions Operations UK Ltd Porous membranes with a polymer grafting, methods and uses thereof
WO2016090295A1 (en) 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground
CA2970174C (en) 2014-12-08 2024-01-23 Berkeley Lights, Inc. Microfluidic device comprising lateral/vertical transistor structures and process of making and using same
EP3229961B1 (en) 2014-12-08 2019-11-13 Berkeley Lights, Inc. Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof
US10578630B2 (en) 2014-12-09 2020-03-03 Berkeley Lights, Inc. Automated identification of assay areas in a microfluidic device and detection of assay positive areas based on rate of change of image light intensity
EP3230717B1 (en) 2014-12-09 2021-08-18 Berkeley Lights, Inc. Automated detection and identification of micro-objects in microfluidic devices
US9744533B2 (en) * 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
SG10202102009QA (en) 2014-12-10 2021-04-29 Berkeley Lights Inc Systems for operating electrokinetic devices
US10101250B2 (en) 2015-04-22 2018-10-16 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
KR102334118B1 (en) 2015-04-22 2021-12-01 버클리 라잇츠, 인크. Freezing and archiving cells on a microfluidic device
US10723988B2 (en) 2015-04-22 2020-07-28 Berkeley Lights, Inc. Microfluidic cell culture
TWI712686B (en) * 2015-04-22 2020-12-11 美商柏克萊燈光有限公司 Culturing station for microfluidic device
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
GB201512600D0 (en) * 2015-07-17 2015-08-26 Koniku Ltd Cell culture, transport and investigation
WO2017065854A2 (en) * 2015-07-22 2017-04-20 The University Of North Carolina At Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
CN115532189A (en) * 2015-11-23 2022-12-30 伯克利之光生命科技公司 In-situ generated microfluidic isolation structure, kit and use method thereof
AU2016365724B2 (en) 2015-12-08 2023-10-12 Berkeley Lights, Inc. Microfluidic devices and kits and methods for use thereof
KR102512607B1 (en) 2015-12-30 2023-03-21 버클리 라잇츠, 인크. Microfluidic devices, kits and methods for optically driven convection and displacement
TWI808934B (en) 2015-12-31 2023-07-21 美商伯克利之光生命科技公司 Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
US11420204B2 (en) 2016-01-13 2022-08-23 Duke University Platforms for single cell analysis
CA3011087A1 (en) * 2016-01-15 2017-07-20 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
EP3429753A4 (en) 2016-03-16 2019-11-06 Berkeley Lights, Inc. Methods, systems and devices for selection and generation of genome edited clones
CN109196094A (en) * 2016-03-17 2019-01-11 伯克利之光生命科技公司 The selection of T lymphocyte and clone in microfluidic device
AU2017239666B2 (en) 2016-03-31 2021-07-29 Berkeley Lights, Inc. Nucleic acid stabilization reagent, kits, and methods of use thereof
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
JP6985289B2 (en) * 2016-04-15 2021-12-22 バークレー ライツ，インコーポレイテッド In-pen assay methods, systems and kits
CN115678773A (en) 2016-05-26 2023-02-03 伯克利之光生命科技公司 Covalently modified surface, kit, preparation method and application
JP7038100B2 (en) * 2016-07-21 2022-03-17 バークレー ライツ，インコーポレイテッド Sorting of T lymphocytes with a microfluidic device
AU2017336164A1 (en) 2016-10-01 2019-04-18 Berkeley Lights, Inc. DNA barcode compositions and methods of in situ identification in a microfluidic device
CN114755412A (en) * 2016-10-23 2022-07-15 伯克利之光生命科技公司 Method for screening B cell lymphocytes
CN110268414B (en) 2016-12-01 2023-08-01 伯克利之光生命科技公司 Automated detection and repositioning of micro-objects in a microfluidic device
AU2017368267B2 (en) 2016-12-01 2022-12-15 Berkeley Lights, Inc. Apparatuses, systems and methods for imaging micro-objects
EP3551745A4 (en) 2016-12-12 2020-07-15 Xcella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
WO2018126205A1 (en) * 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
WO2018208332A2 (en) 2017-02-17 2018-11-15 Koniku, Inc. Systems for detection
EP3635112A2 (en) 2017-06-06 2020-04-15 Zymergen, Inc. A htp genomic engineering platform for improving fungal strains
JP2020528747A (en) 2017-07-21 2020-10-01 バークレー ライツ，インコーポレイテッド Antigen-presenting synthetic surfaces, covalently functionalized surfaces, activated T cells and their use
EP3721209B1 (en) 2017-10-15 2024-02-07 Berkeley Lights, Inc. Methods for in-pen assays
AU2019276567A1 (en) * 2018-05-31 2021-01-28 Berkeley Lights, Inc. Automated detection and characterization of micro-objects in microfluidic devices
BR112020024839A2 (en) 2018-06-06 2021-05-18 Zymergen Inc. manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
EP3853352A4 (en) 2018-09-21 2022-08-10 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
EP3874265A4 (en) * 2018-11-01 2022-09-28 Berkeley Lights, Inc. Methods for assaying biological cells in a microfluidic device
IT201900002777A1 (en) * 2019-02-26 2020-08-26 Menarini Silicon Biosystems Spa METHOD AND MICROFLUIDIC SYSTEM FOR THE ISOLATION OF PARTICLES
WO2020223555A1 (en) 2019-04-30 2020-11-05 Berkeley Lights, Inc. Methods for encapsulating and assaying cells
KR20220113683A (en) 2019-11-17 2022-08-16 버클리 라잇츠, 인크. Systems and methods for analysis of biological samples
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2255599C (en) 1996-04-25 2006-09-05 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1127611A3 (en) * 1999-03-03 2001-11-07 Symyx Technologies, Inc. Chemical processing microsystems with integrated, separation-based reaction product analysis
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
MXPA01012959A (en) 1999-06-28 2002-07-30 California Inst Of Techn Microfabricated elastomeric valve and pump systems.
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
ITTO20010411A1 (en) 2001-05-02 2002-11-02 Silicon Biosystems S R L METHOD AND DEVICE FOR THE EXECUTION OF TESTS AND TESTS WITH HIGH PROCESSIVITY AND HIGH BIOLOGICAL VALUE ON CELLS AND / OR COMPOUNDS.
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
EP2340890B1 (en) 2003-04-03 2016-10-19 Fluidigm Corporation Method of performimg digital PCR
JP4328168B2 (en) 2003-10-02 2009-09-09 ソニー株式会社 Detection unit for interaction between substances using capillary phenomenon, method using the detection unit, and substrate for bioassay
US7425253B2 (en) 2004-01-29 2008-09-16 Massachusetts Institute Of Technology Microscale sorting cytometer
CN1942590B (en) 2004-02-18 2012-09-05 *** Fluidic devices and methods for multiplex chemical and biochemical reactions
US7612355B2 (en) 2004-04-12 2009-11-03 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
JPWO2005121767A1 (en) * 2004-05-25 2008-04-10 有限会社フルイド Microfluidic device and analytical fractionation apparatus using the same
US20060091015A1 (en) * 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
ES2865180T3 (en) 2005-07-07 2021-10-15 Univ California Apparatus for cell culture formation
CN101548004A (en) 2005-08-19 2009-09-30 加利福尼亚大学董事会 Microfluidic methods for diagnostics and cellular analysis
AU2006283518A1 (en) 2005-08-19 2007-03-01 The Regents Of The University Of California Microfluidic methods for diagnostics and cellular analysis
EP1951742A4 (en) 2005-10-27 2011-06-01 Life Technologies Corp Optoelectronic separation of biomolecules
US7964078B2 (en) * 2005-11-07 2011-06-21 The Regents Of The University Of California Microfluidic device for cell and particle separation
US7763453B2 (en) 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
WO2008002462A2 (en) 2006-06-23 2008-01-03 Micronics, Inc. Methods and devices for microfluidic point-of-care immunoassays
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
ES2587007T3 (en) 2006-03-24 2016-10-20 Handylab, Inc. Integrated system to process microfluidic samples, and methods of use thereof
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US9023642B2 (en) 2006-07-07 2015-05-05 The University Of Houston System Method and apparatus for a miniature bioreactor system for long-term cell culture
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
WO2008147530A1 (en) 2007-05-24 2008-12-04 The Regents Of The University Of California Integrated fluidics devices with magnetic sorting
US7736891B2 (en) 2007-09-11 2010-06-15 University Of Washington Microfluidic assay system with dispersion monitoring
US9211537B2 (en) 2007-11-07 2015-12-15 The University Of British Columbia Microfluidic device and method of using same
CA2720324C (en) 2008-04-03 2016-08-23 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
WO2009130694A2 (en) 2008-04-21 2009-10-29 Cell Kinetics Ltd. Flat cell carriers with cell traps
CN101275114A (en) 2008-04-22 2008-10-01 北京大学 Microflow cell culture array and application thereof
KR100991752B1 (en) 2008-07-15 2010-11-03 한국과학기술원 Apparatus and Method for Microparticle Manipulation Using Single Planar Optoelectronic Device
EP2191471A4 (en) 2008-07-28 2013-12-18 Agere Systems Inc Systems and methods for variable compensated fly height measurement
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
EP2414539B1 (en) 2009-04-03 2020-12-16 The Regents of The University of California Apparatus and method for sorting cells and other biological particulates
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (en) 2009-06-19 2011-01-06 Univ Of Tokyo Method for operating particle and micro fluid device
CN102472697A (en) 2009-07-06 2012-05-23 索尼公司 Microfluidic device
WO2011005778A1 (en) 2009-07-06 2011-01-13 Sony Corporation Microfluidic device
US20110143378A1 (en) 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
WO2011149032A1 (en) 2010-05-26 2011-12-01 東ソー株式会社 Biological-sample affixing device
CN101947124B (en) * 2010-06-25 2012-07-04 博奥生物有限公司 Integrated microfluidic chip device and using method thereof
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
EP2616551B1 (en) 2010-09-14 2020-08-19 The Regents of The University of California Method for isolating cells from heterogeneous solution using microfluidic trapping vortices
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
WO2012072823A1 (en) * 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Rapid screening of monoclonal antibodies
US9891157B2 (en) * 2010-12-03 2018-02-13 Cellply S.R.L. Microanalysis of cellular function
EP2714884B1 (en) 2011-05-27 2019-08-21 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
CN103998394B (en) 2011-08-01 2016-08-17 德诺弗科学公司 Cell capture system and using method
US9050593B2 (en) * 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
WO2013148745A1 (en) * 2012-03-28 2013-10-03 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
TWI512383B (en) 2012-07-04 2015-12-11 Ind Tech Res Inst Optically-induced dielectrophoresis device
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
CN115074413A (en) 2013-03-28 2022-09-20 英属哥伦比亚大学 Microfluidic device and method of use thereof in multi-cell secretion detection
AU2014340089B2 (en) 2013-10-22 2019-09-26 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
SG10202002543UA (en) 2013-10-22 2020-05-28 Berkeley Lights Inc Micro-fluidic devices for assaying biological activity
CA2970174C (en) 2014-12-08 2024-01-23 Berkeley Lights, Inc. Microfluidic device comprising lateral/vertical transistor structures and process of making and using same
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
US10723988B2 (en) 2015-04-22 2020-07-28 Berkeley Lights, Inc. Microfluidic cell culture

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