SG11201505901YA - Methods of increasing protein purity using protein a based chromatography - Google Patents
Methods of increasing protein purity using protein a based chromatographyInfo
- Publication number
- SG11201505901YA SG11201505901YA SG11201505901YA SG11201505901YA SG11201505901YA SG 11201505901Y A SG11201505901Y A SG 11201505901YA SG 11201505901Y A SG11201505901Y A SG 11201505901YA SG 11201505901Y A SG11201505901Y A SG 11201505901YA SG 11201505901Y A SG11201505901Y A SG 11201505901YA
- Authority
- SG
- Singapore
- Prior art keywords
- protein
- methods
- based chromatography
- purity
- increasing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
- B01D15/166—Fluid composition conditioning, e.g. gradient
- B01D15/168—Fluid composition conditioning, e.g. gradient pH gradient, chromatofocusing, i.e. separation according to the isoelectric point pI
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361783381P | 2013-03-14 | 2013-03-14 | |
| PCT/US2014/021802 WO2014159064A1 (en) | 2013-03-14 | 2014-03-07 | Methods of increasing protein purity using protein a based chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201505901YA true SG11201505901YA (en) | 2015-08-28 |
Family
ID=50680106
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201505901YA SG11201505901YA (en) | 2013-03-14 | 2014-03-07 | Methods of increasing protein purity using protein a based chromatography |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US10472389B2 (en) |
| EP (1) | EP2970379B1 (en) |
| JP (1) | JP6285532B2 (en) |
| KR (1) | KR101838039B1 (en) |
| CN (1) | CN105073769B (en) |
| CA (1) | CA2903496C (en) |
| DK (1) | DK2970379T3 (en) |
| ES (1) | ES2735354T3 (en) |
| SG (1) | SG11201505901YA (en) |
| WO (1) | WO2014159064A1 (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105073769B (en) | 2013-03-14 | 2019-09-10 | Emd密理博公司 | Increase the method for purity of protein using the chromatography based on A albumen |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| WO2017014261A1 (en) * | 2015-07-22 | 2017-01-26 | 株式会社カネカ | Method for purifying antibody-like protein |
| WO2017014257A1 (en) * | 2015-07-22 | 2017-01-26 | 株式会社カネカ | ANTIBODY-BINDING PROTEIN HAVING REDUCED ANTIBODY-BINDING CAPACITY IN ACIDIC pH REGIONS |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US11753438B2 (en) | 2016-05-11 | 2023-09-12 | Cytiva Bioprocess R&D Ab | Method of cleaning and/or sanitizing a separation matrix |
| JP7106187B2 (en) | 2016-05-11 | 2022-07-26 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | How to save the separation matrix |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| CN109071613A (en) | 2016-05-11 | 2018-12-21 | 通用电气医疗集团生物工艺研发股份公司 | Isolation medium |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69936946T2 (en) | 1998-05-06 | 2008-05-15 | Genentech, Inc., South San Francisco | Purification of antibodies by ion exchange chromatography |
| CA2333747C (en) | 1998-06-01 | 2008-02-19 | Genentech, Inc. | Separation of protein monomers by use of ion-exchange chromatography |
| JPWO2004087761A1 (en) * | 2003-03-31 | 2006-07-27 | 麒麟麦酒株式会社 | Purification of human monoclonal antibodies and human polyclonal antibodies |
| EP2066419B1 (en) * | 2006-09-29 | 2015-11-11 | GE Healthcare Bio-Sciences AB | Chromatography ligand comprising domain c from staphyloccocus aureus protein a for antibody isolation |
| WO2008085988A1 (en) * | 2007-01-05 | 2008-07-17 | Amgen Inc. | Methods of purifying proteins |
| JP5522723B2 (en) * | 2007-05-21 | 2014-06-18 | ノマディックバイオサイエンス株式会社 | Novel polypeptide, material for affinity chromatography, and method for separation and / or purification of immunoglobulin |
| US20100069617A1 (en) | 2008-09-12 | 2010-03-18 | Ge Healthcare Bio-Sciences Ab | Enhanced protein aggregate removal by mixed mode chromatography on hydrophobic interaction media in the presence of protein-excluded zwitterions |
| SG162687A1 (en) * | 2008-12-24 | 2010-07-29 | Millipore Corp | Caustic stable chromatography ligands |
| EP2412809B1 (en) * | 2009-03-24 | 2017-08-09 | Kaneka Corporation | Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand |
| MX2011014008A (en) * | 2009-06-26 | 2012-06-01 | Regeneron Pharma | Readily isolated bispecific antibodies with native immunoglobulin format. |
| EP2473617B1 (en) * | 2009-09-01 | 2020-02-26 | F.Hoffmann-La Roche Ag | Enhanced protein purification through a modified protein a elution |
| SG193378A1 (en) | 2011-03-16 | 2013-10-30 | Hoffmann La Roche | Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase |
| SG186552A1 (en) | 2011-06-08 | 2013-01-30 | Emd Millipore Corp | Chromatography matrices including novel staphylococcus aureus protein a based ligands |
| US9657055B2 (en) * | 2011-11-30 | 2017-05-23 | Ge Healthcare Bioprocess R&D Ab | Affinity chromatography matrix |
| CN105073769B (en) | 2013-03-14 | 2019-09-10 | Emd密理博公司 | Increase the method for purity of protein using the chromatography based on A albumen |
-
2014
- 2014-03-07 CN CN201480013955.9A patent/CN105073769B/en active Active
- 2014-03-07 DK DK14722399.4T patent/DK2970379T3/en active
- 2014-03-07 SG SG11201505901YA patent/SG11201505901YA/en unknown
- 2014-03-07 KR KR1020157022870A patent/KR101838039B1/en active IP Right Grant
- 2014-03-07 ES ES14722399T patent/ES2735354T3/en active Active
- 2014-03-07 CA CA2903496A patent/CA2903496C/en active Active
- 2014-03-07 JP JP2016500853A patent/JP6285532B2/en active Active
- 2014-03-07 WO PCT/US2014/021802 patent/WO2014159064A1/en active Application Filing
- 2014-03-07 EP EP14722399.4A patent/EP2970379B1/en active Active
- 2014-03-07 US US14/768,254 patent/US10472389B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP6285532B2 (en) | 2018-02-28 |
| US10472389B2 (en) | 2019-11-12 |
| EP2970379A1 (en) | 2016-01-20 |
| ES2735354T3 (en) | 2019-12-18 |
| CA2903496A1 (en) | 2014-10-02 |
| KR20150110731A (en) | 2015-10-02 |
| WO2014159064A1 (en) | 2014-10-02 |
| CN105073769B (en) | 2019-09-10 |
| CN105073769A (en) | 2015-11-18 |
| EP2970379B1 (en) | 2019-05-01 |
| US20160122385A1 (en) | 2016-05-05 |
| KR101838039B1 (en) | 2018-03-14 |
| CA2903496C (en) | 2020-01-14 |
| JP2016518318A (en) | 2016-06-23 |
| DK2970379T3 (en) | 2019-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SG11201505901YA (en) | Methods of increasing protein purity using protein a based chromatography | |
| SG11201510747RA (en) | Method for increasing expression of rna-encoded proteins | |
| GB201317685D0 (en) | PRESS-ON footwear aid | |
| SG11201510788XA (en) | Protein a chromatography | |
| SG11201505198WA (en) | Protein purification methods | |
| SG11201603028SA (en) | Purification of proteins | |
| EP2780789A4 (en) | Chromatography configuration interface | |
| IL239737A0 (en) | Method of producing a protein | |
| GB201302597D0 (en) | Novel Synthetic Proteins | |
| IL245265B (en) | Antibodies specific to fcrn | |
| IL268868B (en) | Novel method of protein purification | |
| EP2976353A4 (en) | Autophagy-inducing peptide analogs | |
| HK1220903A1 (en) | Cartilage-binding fusion proteins | |
| PT3061771T (en) | Novel four-ctl epitope-joined peptide | |
| GB201308057D0 (en) | Protein | |
| HK1213795A1 (en) | Purification of proteins using hydrophobic interaction chromatography | |
| HK1211945A1 (en) | Improved purification of proteins via a deglycosylation step | |
| HK1198769A1 (en) | Novel peptide | |
| GB201401955D0 (en) | Protein | |
| HK1220194A1 (en) | Polymorph forms of desazadesferrithiocin analogs (desazadesferrithiocin) | |
| GB2519395B (en) | Chromatographic interface | |
| SG10201403600SA (en) | Chromatography column | |
| EP2978770A4 (en) | Refolding of proteins | |
| GB201314945D0 (en) | Protein crystals | |
| GB201317022D0 (en) | Polymorphs |