SG10201710049RA - Compositions, methods and systems for polymerase chain reaction assays - Google Patents
Compositions, methods and systems for polymerase chain reaction assaysInfo
- Publication number
- SG10201710049RA SG10201710049RA SG10201710049RA SG10201710049RA SG10201710049RA SG 10201710049R A SG10201710049R A SG 10201710049RA SG 10201710049R A SG10201710049R A SG 10201710049RA SG 10201710049R A SG10201710049R A SG 10201710049RA SG 10201710049R A SG10201710049R A SG 10201710049RA
- Authority
- SG
- Singapore
- Prior art keywords
- compositions
- systems
- methods
- chain reaction
- polymerase chain
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361775415P | 2013-03-08 | 2013-03-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201710049RA true SG10201710049RA (en) | 2018-01-30 |
Family
ID=51492033
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10201710049RA SG10201710049RA (en) | 2013-03-08 | 2014-03-07 | Compositions, methods and systems for polymerase chain reaction assays |
SG11201507087WA SG11201507087WA (en) | 2013-03-08 | 2014-03-07 | Compositions, methods and systems for polymerase chain reaction assays |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201507087WA SG11201507087WA (en) | 2013-03-08 | 2014-03-07 | Compositions, methods and systems for polymerase chain reaction assays |
Country Status (6)
Country | Link |
---|---|
US (2) | US9822393B2 (en) |
EP (2) | EP2964787B1 (en) |
CN (2) | CN105189785B (en) |
AU (1) | AU2014225372B2 (en) |
SG (2) | SG10201710049RA (en) |
WO (1) | WO2014138711A1 (en) |
Families Citing this family (79)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9156010B2 (en) * | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
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US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9567631B2 (en) | 2012-12-14 | 2017-02-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
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CN105189785B (en) | 2013-03-08 | 2018-04-20 | 伯乐生命医学产品有限公司 | Composition, method and system for polymerase chain reaction measure |
US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
CN106413896B (en) | 2014-04-10 | 2019-07-05 | 10X基因组学有限公司 | For encapsulating and dividing fluid means, system and method and its application of reagent |
EP3889325A1 (en) | 2014-06-26 | 2021-10-06 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
US10839939B2 (en) | 2014-06-26 | 2020-11-17 | 10X Genomics, Inc. | Processes and systems for nucleic acid sequence assembly |
MX2017005267A (en) | 2014-10-29 | 2017-07-26 | 10X Genomics Inc | Methods and compositions for targeted nucleic acid sequencing. |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
AU2016207023B2 (en) | 2015-01-12 | 2019-12-05 | 10X Genomics, Inc. | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
KR20170106979A (en) | 2015-01-13 | 2017-09-22 | 10엑스 제노믹스, 인크. | System and method for visualizing structure variation and phase adjustment information |
JP2018513445A (en) | 2015-02-09 | 2018-05-24 | 10エックス ゲノミクス,インコーポレイテッド | System and method for fading using structural variation identification and variant call data |
WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
EP3262188B1 (en) | 2015-02-24 | 2021-05-05 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
JP6954899B2 (en) | 2015-12-04 | 2021-10-27 | 10エックス ゲノミクス,インコーポレイテッド | Methods and compositions for nucleic acid analysis |
US10730030B2 (en) | 2016-01-08 | 2020-08-04 | Bio-Rad Laboratories, Inc. | Multiple beads per droplet resolution |
CN109526227A (en) | 2016-01-25 | 2019-03-26 | 生物辐射欧洲有限公司 | Digital microbiology |
JP6735348B2 (en) | 2016-02-11 | 2020-08-05 | 10エックス ジェノミクス, インコーポレイテッド | Systems, methods and media for de novo assembly of whole genome sequence data |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
CN110291209A (en) * | 2016-11-10 | 2019-09-27 | 卡尤迪生物科技(北京)有限公司 | For carrying out the method and system of chemical or biological reactionss |
WO2018118978A1 (en) | 2016-12-20 | 2018-06-28 | Bio-Rad Laboratories, Inc. | Uv graft on microfluidic devices |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
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US10919038B2 (en) | 2016-12-28 | 2021-02-16 | Bio-Rad Laboratories, Inc. | Modification of surface properties of microfluidic devices |
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WO2018140966A1 (en) | 2017-01-30 | 2018-08-02 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
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US20180340169A1 (en) | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
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US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | Methods and systems for nuclecic acid preparation and chromatin analysis |
CN111479631B (en) | 2017-10-27 | 2022-02-22 | 10X基因组学有限公司 | Methods and systems for sample preparation and analysis |
SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
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WO2019157529A1 (en) | 2018-02-12 | 2019-08-15 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
EP3775271A1 (en) | 2018-04-06 | 2021-02-17 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
TWI725686B (en) | 2018-12-26 | 2021-04-21 | 財團法人工業技術研究院 | Tubular structure for producing droplets and method for producing droplets |
US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
WO2020168013A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
WO2020185791A1 (en) | 2019-03-11 | 2020-09-17 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
CN110218776A (en) * | 2019-06-14 | 2019-09-10 | 苏州叠代生物科技有限公司 | PCR oil phase composition and preparation method thereof |
CN110218777B (en) * | 2019-06-14 | 2023-05-16 | 苏州天合优才生物科技有限公司 | PCR premix |
CN110511981A (en) * | 2019-09-09 | 2019-11-29 | 中国科学院苏州生物医学工程技术研究所 | Using the method for digital pcr evaluation DNA polymerase activity |
CN110578000A (en) * | 2019-09-09 | 2019-12-17 | 中国科学院苏州生物医学工程技术研究所 | method for detecting long non-coding RNA in urine of prostate cancer patient by using digital PCR |
US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
US20230249178A1 (en) | 2020-07-08 | 2023-08-10 | Roche Sequencing Solutions, Inc. | Split-pool synthesis apparatus and methods of performing split-pool synthesis |
EP4228793A1 (en) | 2020-10-15 | 2023-08-23 | Kapa Biosystems, Inc. | Electrophoretic devices and methods for next-generation sequencing library preparation |
WO2022182682A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
WO2022194764A1 (en) | 2021-03-15 | 2022-09-22 | F. Hoffmann-La Roche Ag | Targeted next-generation sequencing via anchored primer extension |
WO2022200485A1 (en) | 2021-03-26 | 2022-09-29 | F. Hoffmann-La Roche Ag | Hybridization buffer formulations |
CN113351110B (en) * | 2021-05-19 | 2022-08-26 | 大连理工大学 | Temperature-sensitive fluorocarbon surfactant and preparation method and application thereof |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008021123A1 (en) | 2006-08-07 | 2008-02-21 | President And Fellows Of Harvard College | Fluorocarbon emulsion stabilizing surfactants |
WO2010009365A1 (en) * | 2008-07-18 | 2010-01-21 | Raindance Technologies, Inc. | Droplet libraries |
US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
CN101367932A (en) * | 2008-10-10 | 2009-02-18 | 张永明 | Fluorine-containing block polymer and synthesis thereof |
WO2010128157A1 (en) * | 2009-05-07 | 2010-11-11 | Universite De Strasbourg | Microfluidic system and methods for highly selective droplet fusion |
CN101724295B (en) * | 2009-12-03 | 2013-03-13 | 中国农业大学 | LE Green mixed dye and applications thereof |
CN102762611B (en) * | 2009-12-18 | 2015-11-25 | 索尔维特殊聚合物意大利有限公司 | Produce the method for fluoropolymer |
EP2513173B1 (en) * | 2009-12-18 | 2013-09-25 | Solvay Specialty Polymers Italy S.p.A. | Process for manufacturing a dispersion of a vinylidene fluoride polymer |
JP5934657B2 (en) | 2010-02-12 | 2016-06-15 | レインダンス テクノロジーズ, インコーポレイテッド | Digital specimen analysis |
WO2012109604A1 (en) | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Thermocycling device for nucleic acid amplification and methods of use |
WO2012109600A2 (en) | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
CN105189785B (en) * | 2013-03-08 | 2018-04-20 | 伯乐生命医学产品有限公司 | Composition, method and system for polymerase chain reaction measure |
-
2014
- 2014-03-07 CN CN201480026003.0A patent/CN105189785B/en active Active
- 2014-03-07 US US14/201,752 patent/US9822393B2/en active Active
- 2014-03-07 CN CN201810281734.XA patent/CN108441541A/en active Pending
- 2014-03-07 SG SG10201710049RA patent/SG10201710049RA/en unknown
- 2014-03-07 SG SG11201507087WA patent/SG11201507087WA/en unknown
- 2014-03-07 AU AU2014225372A patent/AU2014225372B2/en active Active
- 2014-03-07 WO PCT/US2014/022163 patent/WO2014138711A1/en active Application Filing
- 2014-03-07 EP EP14759550.8A patent/EP2964787B1/en active Active
- 2014-03-07 EP EP18187279.7A patent/EP3418398B1/en active Active
-
2017
- 2017-11-20 US US15/818,601 patent/US10676778B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
AU2014225372A1 (en) | 2015-09-24 |
US10676778B2 (en) | 2020-06-09 |
WO2014138711A1 (en) | 2014-09-12 |
CN108441541A (en) | 2018-08-24 |
EP3418398A1 (en) | 2018-12-26 |
EP3418398B1 (en) | 2020-05-13 |
EP2964787A1 (en) | 2016-01-13 |
US20140302503A1 (en) | 2014-10-09 |
AU2014225372B2 (en) | 2019-10-24 |
CN105189785A (en) | 2015-12-23 |
EP2964787B1 (en) | 2018-09-12 |
EP2964787A4 (en) | 2016-08-17 |
SG11201507087WA (en) | 2015-10-29 |
CN105189785B (en) | 2018-04-20 |
US9822393B2 (en) | 2017-11-21 |
US20180135100A1 (en) | 2018-05-17 |
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