SG10201609752YA - Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris - Google Patents

Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

Info

Publication number
SG10201609752YA
SG10201609752YA SG10201609752YA SG10201609752YA SG10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA
Authority
SG
Singapore
Prior art keywords
titer
antibodies
pichia pastoris
subunit proteins
purity production
Prior art date
Application number
SG10201609752YA
Inventor
Danielle Marie Mitchell
Leon F Garcia-Martinez
Patricia Dianne Mcneill
Ethan Wayne Ojala
Mehmet Inan
John Latham
Original Assignee
Alderbio Holdings Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/466,795 external-priority patent/US11214610B2/en
Application filed by Alderbio Holdings Llc filed Critical Alderbio Holdings Llc
Publication of SG10201609752YA publication Critical patent/SG10201609752YA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10201609752YA 2011-08-19 2012-08-20 Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris SG10201609752YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161525307P 2011-08-19 2011-08-19
US13/466,795 US11214610B2 (en) 2010-12-01 2012-05-08 High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris

Publications (1)

Publication Number Publication Date
SG10201609752YA true SG10201609752YA (en) 2017-01-27

Family

ID=48802355

Family Applications (2)

Application Number Title Priority Date Filing Date
SG2014011878A SG2014011878A (en) 2011-08-19 2012-08-20 Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris
SG10201609752YA SG10201609752YA (en) 2011-08-19 2012-08-20 Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG2014011878A SG2014011878A (en) 2011-08-19 2012-08-20 Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris

Country Status (14)

Country Link
EP (1) EP2744903B1 (en)
JP (1) JP6138788B2 (en)
KR (1) KR101978534B1 (en)
CN (1) CN103890178A (en)
AR (1) AR087612A1 (en)
AU (1) AU2012299035B2 (en)
BR (1) BR112014003841B1 (en)
CA (1) CA2845579C (en)
DK (1) DK2744903T3 (en)
IL (1) IL230978B (en)
MX (1) MX355890B (en)
SG (2) SG2014011878A (en)
TW (1) TWI666318B (en)
WO (1) WO2013028635A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2905180C (en) * 2013-03-15 2023-03-07 Alder Biopharmaceuticals, Inc. Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
US10202630B2 (en) 2013-03-15 2019-02-12 Alderbio Holdings Llc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
TWI670279B (en) * 2013-03-15 2019-09-01 美商艾爾德生物製藥股份有限公司 Antibody purification and purity monitoring
JP6206873B2 (en) * 2013-09-30 2017-10-04 国立研究開発法人農業・食品産業技術総合研究機構 Method for producing porcine lysozyme using secretory signal peptide derived from chicken lysozyme
US10556945B2 (en) 2014-03-21 2020-02-11 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
JP6568099B2 (en) 2014-03-21 2019-08-28 テバ・ファーマシューティカルズ・インターナショナル・ゲーエムベーハーTeva Pharmaceuticals International GmbH Antagonist antibody against calcitonin gene-related peptide and method of use thereof
KR20220031944A (en) 2016-09-23 2022-03-14 테바 파마슈티컬스 인터내셔널 게엠베하 Treating refractory migraine
EP4202035A1 (en) 2021-12-22 2023-06-28 Gelita AG Improved expression of peptides

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
WO1994021293A1 (en) 1993-03-19 1994-09-29 Duke University Method of treatment of tumors with an antibody binding to tenascin
AU3272695A (en) 1994-08-12 1996-03-07 Immunomedics Inc. Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells
US5955349A (en) 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5736383A (en) 1996-08-26 1998-04-07 Zymogenetics, Inc. Preparation of Pichia methanolica auxotrophic mutants
US6730499B1 (en) 1998-07-03 2004-05-04 Research Corporation Technologies, Inc. Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1
WO2000023579A1 (en) 1998-10-22 2000-04-27 The Regents Of The University Of California Functionally assembled antigen-specific intact recombinant antibody and a method for production thereof
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
EP1678314B1 (en) 2003-10-22 2012-09-05 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US8222386B2 (en) * 2006-10-10 2012-07-17 Keck Graduate Institute P. pastoris ADH promoter and use thereof to direct expression of proteins
AU2008254578B2 (en) 2007-05-21 2013-06-06 Alderbio Holdings Llc Novel rabbit antibody humanization methods and humanized rabbit antibodies
WO2008144763A2 (en) * 2007-05-21 2008-11-27 Alder Biopharmaceuticals, Inc. Antibodies to il-6 and use thereof
US11214610B2 (en) 2010-12-01 2022-01-04 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris

Also Published As

Publication number Publication date
AU2012299035B2 (en) 2017-11-02
TW201313899A (en) 2013-04-01
CA2845579C (en) 2020-03-24
AR087612A1 (en) 2014-04-03
KR20140057613A (en) 2014-05-13
CA2845579A1 (en) 2013-02-28
JP2014525247A (en) 2014-09-29
KR101978534B1 (en) 2019-08-28
MX355890B (en) 2018-05-03
EP2744903A4 (en) 2014-12-31
SG2014011878A (en) 2014-04-28
CN103890178A (en) 2014-06-25
BR112014003841A2 (en) 2017-03-14
IL230978B (en) 2018-08-30
MX2014001951A (en) 2016-02-05
IL230978A0 (en) 2014-03-31
BR112014003841B1 (en) 2022-02-15
AU2012299035A1 (en) 2014-03-13
DK2744903T3 (en) 2019-01-28
NZ721102A (en) 2020-01-31
TWI666318B (en) 2019-07-21
NZ621199A (en) 2016-07-29
WO2013028635A1 (en) 2013-02-28
EP2744903A1 (en) 2014-06-25
JP6138788B2 (en) 2017-05-31
WO2013028635A9 (en) 2013-09-26
EP2744903B1 (en) 2018-10-10

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