SG10201407645YA - Methods of Enhancing Pluripotentcy - Google Patents

Methods of Enhancing Pluripotentcy

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Publication number
SG10201407645YA
SG10201407645YA SG10201407645YA SG10201407645YA SG10201407645YA SG 10201407645Y A SG10201407645Y A SG 10201407645YA SG 10201407645Y A SG10201407645Y A SG 10201407645YA SG 10201407645Y A SG10201407645Y A SG 10201407645YA SG 10201407645Y A SG10201407645Y A SG 10201407645YA
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SG
Singapore
Prior art keywords
cell
enhancing
pluripotentcy
methods
tbx3
Prior art date
Application number
SG10201407645YA
Inventor
Lim Bing
Han Jianyong
Tam Wai-Leong
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Agency Science Tech & Res
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Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Priority to SG10201407645YA priority Critical patent/SG10201407645YA/en
Publication of SG10201407645YA publication Critical patent/SG10201407645YA/en

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/604—Klf-4
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    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METHODS OF ENHANCING PLURIPOTENTCY We provide for the use of Tbx3 (GenBank Accession Number: NM_005996.3, NP_005987.3, NM_016569.3, NP_057653.3) in a method of enhancing or inducing pluripotency in a cell such as a somatic cell. We describe a method of reprogramming a cell, 5 the method comprising modulating the expression and/or activity of Tbx3 in the cell. The cell may become a pluripotent cell such as a stem cell. We further describe a method of causing a cell such as a somatic cell to display one or more characteristics of a pluripotent cell, the method comprising modulating the expression and/or activity of Tbx3 in the cell. The method may further comprise modulating the expression and/or activity of one or more, a combination 10 of or all of Oct4, Sox2 and Klf4 in the cell. Figure 4D
SG10201407645YA 2009-11-19 2010-11-18 Methods of Enhancing Pluripotentcy SG10201407645YA (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SG10201407645YA SG10201407645YA (en) 2009-11-19 2010-11-18 Methods of Enhancing Pluripotentcy

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26274109P 2009-11-19 2009-11-19
SG2010008258 2010-02-06
SG10201407645YA SG10201407645YA (en) 2009-11-19 2010-11-18 Methods of Enhancing Pluripotentcy

Publications (1)

Publication Number Publication Date
SG10201407645YA true SG10201407645YA (en) 2015-01-29

Family

ID=54193734

Family Applications (1)

Application Number Title Priority Date Filing Date
SG10201407645YA SG10201407645YA (en) 2009-11-19 2010-11-18 Methods of Enhancing Pluripotentcy

Country Status (5)

Country Link
US (1) US8889412B2 (en)
EP (1) EP2501803B1 (en)
JP (1) JP5939985B2 (en)
SG (1) SG10201407645YA (en)
WO (1) WO2011062559A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5924741B2 (en) 2011-05-27 2016-05-25 公立大学法人横浜市立大学 Method for producing artificial cancer stem cell and method for inducing differentiation thereof
JP6449138B2 (en) 2015-07-02 2019-01-09 株式会社豊田中央研究所 Method for inducing genetic recombination and use thereof
WO2023196998A2 (en) * 2022-04-07 2023-10-12 The Board Of Regents Of The University Of Texas System COMPOSITIONS AND METHODS FOR TREATING LIVER DISEASES WITH siRNAS TARGETING TBX3

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI109814B (en) 2001-11-23 2002-10-15 Vaahto Oy Paper or cardboard web forming apparatus, has wire loop with dewatering box the bottom of which forms fraction line consisting of straight portions and edges between them, over which web between the two wires moves
EA014166B1 (en) 2005-12-13 2010-10-29 Киото Юниверсити Nuclear reprogramming factor
CA2660123C (en) * 2007-10-31 2017-05-09 Kyoto University Nuclear reprogramming method

Also Published As

Publication number Publication date
EP2501803B1 (en) 2020-12-23
US8889412B2 (en) 2014-11-18
JP5939985B2 (en) 2016-06-29
EP2501803A1 (en) 2012-09-26
JP2013511274A (en) 2013-04-04
EP2501803A4 (en) 2013-08-07
WO2011062559A1 (en) 2011-05-26
US20130102479A1 (en) 2013-04-25
WO2011062559A8 (en) 2012-05-18

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