SE9303822D0 - Method of purification - Google Patents

Method of purification

Info

Publication number
SE9303822D0
SE9303822D0 SE9303822A SE9303822A SE9303822D0 SE 9303822 D0 SE9303822 D0 SE 9303822D0 SE 9303822 A SE9303822 A SE 9303822A SE 9303822 A SE9303822 A SE 9303822A SE 9303822 D0 SE9303822 D0 SE 9303822D0
Authority
SE
Sweden
Prior art keywords
protein
pct
native
native protein
hydroxamate
Prior art date
Application number
SE9303822A
Other languages
Swedish (sv)
Inventor
Per Wikstroem
Original Assignee
Kabi Pharmacia Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabi Pharmacia Ab filed Critical Kabi Pharmacia Ab
Priority to SE9303822A priority Critical patent/SE9303822D0/en
Publication of SE9303822D0 publication Critical patent/SE9303822D0/en
Priority to US08/635,908 priority patent/US5912329A/en
Priority to EP95901671A priority patent/EP0738276A1/en
Priority to CA002175591A priority patent/CA2175591A1/en
Priority to AU10814/95A priority patent/AU683966B2/en
Priority to JP7514396A priority patent/JPH09505078A/en
Priority to PCT/SE1994/001088 priority patent/WO1995014035A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Abstract

PCT No. PCT/SE94/01088 Sec. 371 Date Apr. 25, 1996 Sec. 102(e) Date Apr. 25, 1996 PCT Filed Nov. 17, 1994 PCT Pub. No. WO95/14035 PCT Pub. Date May 26, 1995The invention relates to a method for purification of a mixture of hydroxamate derivatized protein/proteins and native protein which is characterized by treating the mixture with immobilized metal and thereby adsorbing the hydroxamate derivatized protein/proteins on the immobilized-metal and recovering the native protein. The protein could be IGF-L. It also relates to a process for the production of a native protein which is characterized by expression of the protein as a fusion protein, cleavage of the fusion protein by hydroxylanine, separation of native protein from hydroxamate derivatized protein by adsorbing the hydroxamate derivatized protein on immobilized metal and directly recovering the native protein. The use of immobilized metal affinity chromatography for separation of native protein from hydroxamate derivatized protein is also claimed.
SE9303822A 1993-11-18 1993-11-18 Method of purification SE9303822D0 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
SE9303822A SE9303822D0 (en) 1993-11-18 1993-11-18 Method of purification
US08/635,908 US5912329A (en) 1993-11-18 1994-11-17 Method for purification of a mixture of hydroxamate derivatized protein and native protein
EP95901671A EP0738276A1 (en) 1993-11-18 1994-11-17 Method for purification of a mixture of hydroxamate derivatized protein and native protein
CA002175591A CA2175591A1 (en) 1993-11-18 1994-11-17 Method for purification of a mixture of hydroxamate derivatized protein and native protein
AU10814/95A AU683966B2 (en) 1993-11-18 1994-11-17 Method for purification of a mixture of hydroxamate derivatized protein and native protein
JP7514396A JPH09505078A (en) 1993-11-18 1994-11-17 Method for purifying a mixture of hydroxamate-derived protein and native protein
PCT/SE1994/001088 WO1995014035A1 (en) 1993-11-18 1994-11-17 Method for purification of a mixture of hydroxamate derivatized protein and native protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9303822A SE9303822D0 (en) 1993-11-18 1993-11-18 Method of purification

Publications (1)

Publication Number Publication Date
SE9303822D0 true SE9303822D0 (en) 1993-11-18

Family

ID=20391799

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9303822A SE9303822D0 (en) 1993-11-18 1993-11-18 Method of purification

Country Status (7)

Country Link
US (1) US5912329A (en)
EP (1) EP0738276A1 (en)
JP (1) JPH09505078A (en)
AU (1) AU683966B2 (en)
CA (1) CA2175591A1 (en)
SE (1) SE9303822D0 (en)
WO (1) WO1995014035A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020074749A (en) * 2001-03-21 2002-10-04 한국생명공학연구원 Large scale production of recombinant human insulin like growth factor-1

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE451802B (en) * 1984-05-10 1987-11-02 Jerker Porath PRODUCT FOR ADSORPTION OF METAL IONS
US4569794A (en) * 1984-12-05 1986-02-11 Eli Lilly And Company Process for purifying proteins and compounds useful in such process
CA1304886C (en) * 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
US5169936A (en) * 1989-04-14 1992-12-08 Biogen, Inc. Protein purification on immobilized metal affinity resins effected by elution using a weak ligand

Also Published As

Publication number Publication date
CA2175591A1 (en) 1995-05-26
WO1995014035A1 (en) 1995-05-26
EP0738276A1 (en) 1996-10-23
AU683966B2 (en) 1997-11-27
AU1081495A (en) 1995-06-06
US5912329A (en) 1999-06-15
JPH09505078A (en) 1997-05-20

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