SE9300231L - Methods to determine the concentration of an analyte in a liquid chromatographic or capillary electrophoretic flow by measuring the refractive index of the liquid - Google Patents

Methods to determine the concentration of an analyte in a liquid chromatographic or capillary electrophoretic flow by measuring the refractive index of the liquid

Info

Publication number
SE9300231L
SE9300231L SE9300231A SE9300231A SE9300231L SE 9300231 L SE9300231 L SE 9300231L SE 9300231 A SE9300231 A SE 9300231A SE 9300231 A SE9300231 A SE 9300231A SE 9300231 L SE9300231 L SE 9300231L
Authority
SE
Sweden
Prior art keywords
liquid
analyte
refractive index
measuring
concentration
Prior art date
Application number
SE9300231A
Other languages
Swedish (sv)
Other versions
SE500817C2 (en
SE9300231D0 (en
Inventor
Anders Hanning
Original Assignee
Anders Hanning
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anders Hanning filed Critical Anders Hanning
Priority to SE9300231A priority Critical patent/SE500817C2/en
Publication of SE9300231D0 publication Critical patent/SE9300231D0/en
Priority to EP94905892A priority patent/EP0681694A1/en
Priority to PCT/SE1994/000045 priority patent/WO1994017393A1/en
Priority to AU59817/94A priority patent/AU5981794A/en
Publication of SE9300231L publication Critical patent/SE9300231L/en
Publication of SE500817C2 publication Critical patent/SE500817C2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N2030/621—Detectors specially adapted therefor signal-to-noise ratio
    • G01N2030/625—Detectors specially adapted therefor signal-to-noise ratio by measuring reference material, e.g. carrier without sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8429—Preparation of the fraction to be distributed adding modificating material
    • G01N2030/8441—Preparation of the fraction to be distributed adding modificating material to modify physical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/4133—Refractometers, e.g. differential

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

In a method of determining an analyte in a liquid chromatographic or capillary electrophoretic separation by measuring bulk refractive index, the analyte is labeled with a species having a high refractive index at the or at least one measuring wavelength.
SE9300231A 1993-01-27 1993-01-27 Methods to determine the concentration of an analyte in a liquid chromatographic or capillary electrophoretic flow by measuring the refractive index of the liquid SE500817C2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
SE9300231A SE500817C2 (en) 1993-01-27 1993-01-27 Methods to determine the concentration of an analyte in a liquid chromatographic or capillary electrophoretic flow by measuring the refractive index of the liquid
EP94905892A EP0681694A1 (en) 1993-01-27 1994-01-21 An improved method of liquid bulk refractive index detection
PCT/SE1994/000045 WO1994017393A1 (en) 1993-01-27 1994-01-21 An improved method of liquid bulk refractive index detection
AU59817/94A AU5981794A (en) 1993-01-27 1994-01-21 An improved method of liquid bulk refractive index detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9300231A SE500817C2 (en) 1993-01-27 1993-01-27 Methods to determine the concentration of an analyte in a liquid chromatographic or capillary electrophoretic flow by measuring the refractive index of the liquid

Publications (3)

Publication Number Publication Date
SE9300231D0 SE9300231D0 (en) 1993-01-27
SE9300231L true SE9300231L (en) 1994-07-28
SE500817C2 SE500817C2 (en) 1994-09-12

Family

ID=20388665

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9300231A SE500817C2 (en) 1993-01-27 1993-01-27 Methods to determine the concentration of an analyte in a liquid chromatographic or capillary electrophoretic flow by measuring the refractive index of the liquid

Country Status (4)

Country Link
EP (1) EP0681694A1 (en)
AU (1) AU5981794A (en)
SE (1) SE500817C2 (en)
WO (1) WO1994017393A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9500356D0 (en) * 1995-02-01 1995-02-01 Anders Hanning Method and apparatus for optical characterization of liquids
SE9602788L (en) * 1996-07-16 1998-01-17 Anders Hanning Improved refractometric method
DE102009033426A1 (en) * 2009-07-16 2011-02-03 Technische Universität München Electrophoresis-measuring device for detecting drift motion of electrically charged particles in electrical field for separation and analysis of e.g. ionized species, has processing device determining drift motion of charged particles
CN101776572B (en) * 2010-03-05 2011-07-20 陕西师范大学 Liquid refractive index CCD real-time measuring device and measuring method
CN101782515B (en) * 2010-03-05 2011-07-20 陕西师范大学 Method for measuring liquid refractive rate based on total reflection diaphragm effect

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4213699A (en) * 1976-02-27 1980-07-22 S.A. Texaco Belgium N.V. Method of measuring low concentrations of a light absorbing component
US4381895A (en) * 1980-02-28 1983-05-03 Biovation, Inc. Method and apparatus for automatic flow-through digital refractometer
US4704029A (en) * 1985-12-26 1987-11-03 Research Corporation Blood glucose monitor
US4952055A (en) * 1988-10-03 1990-08-28 Wyatt Technology Corporation Differential refractometer
SE9200917D0 (en) * 1991-08-20 1992-03-25 Pharmacia Biosensor Ab ASSAY METHOD

Also Published As

Publication number Publication date
SE500817C2 (en) 1994-09-12
WO1994017393A1 (en) 1994-08-04
EP0681694A1 (en) 1995-11-15
SE9300231D0 (en) 1993-01-27
AU5981794A (en) 1994-08-15

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