SE9201615L - Bioimmunological method for the determination of tPa and PAI-1 - Google Patents

Bioimmunological method for the determination of tPa and PAI-1

Info

Publication number
SE9201615L
SE9201615L SE9201615A SE9201615A SE9201615L SE 9201615 L SE9201615 L SE 9201615L SE 9201615 A SE9201615 A SE 9201615A SE 9201615 A SE9201615 A SE 9201615A SE 9201615 L SE9201615 L SE 9201615L
Authority
SE
Sweden
Prior art keywords
tpa
pai
activity
sample
solid phase
Prior art date
Application number
SE9201615A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE9201615D0 (en
Inventor
Steffen Rosen
Original Assignee
Chromogenix Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromogenix Ab filed Critical Chromogenix Ab
Priority to SE9201615A priority Critical patent/SE9201615L/en
Publication of SE9201615D0 publication Critical patent/SE9201615D0/en
Priority to AU40998/93A priority patent/AU4099893A/en
Priority to PCT/SE1993/000449 priority patent/WO1993023561A1/en
Publication of SE9201615L publication Critical patent/SE9201615L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
    • G01N2333/8121—Serpins
    • G01N2333/8132—Plasminogen activator inhibitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/972—Plasminogen activators
    • G01N2333/9726—Tissue plasminogen activator

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Neurosurgery (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An in vitro method for assaying tPA-activity, optionally in combination with assaying PAI-1-activity. The method is characterized in that it includes the steps (i) of incubating a sample containing tPA with a solid phase that specifically binds tPA under pH conditions which inhibit the formation of a tPA-PAI-1 complex, whereafter (ii) the tPA-activity of the solid phase is determined. The pH conditions mean that PAI-1 will not be denatured irreversibly. If PAI-1 is to be determined functionally, the sample is conditioned with regard to pH prior to step (i). A first portion and a second portion of the sample are then incubated in accordance with step (i) with the exception of the pH conditions for the second portion, which are selected so that a PAI-1-tPA complex can form. The activity of the tPA bound to the solid phase is determined separately for both portions as a final stage, and the PAI-1-activity is calculated from the ratio between the measured tPA-activity of the different sample portions.
SE9201615A 1992-05-21 1992-05-21 Bioimmunological method for the determination of tPa and PAI-1 SE9201615L (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE9201615A SE9201615L (en) 1992-05-21 1992-05-21 Bioimmunological method for the determination of tPa and PAI-1
AU40998/93A AU4099893A (en) 1992-05-21 1993-05-19 Bioimmunoassay for tPA and PAI-1
PCT/SE1993/000449 WO1993023561A1 (en) 1992-05-21 1993-05-19 BIOIMMUNOASSAY FOR tPA AND PAI-1

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9201615A SE9201615L (en) 1992-05-21 1992-05-21 Bioimmunological method for the determination of tPa and PAI-1

Publications (2)

Publication Number Publication Date
SE9201615D0 SE9201615D0 (en) 1992-05-21
SE9201615L true SE9201615L (en) 1993-11-22

Family

ID=20386314

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9201615A SE9201615L (en) 1992-05-21 1992-05-21 Bioimmunological method for the determination of tPa and PAI-1

Country Status (3)

Country Link
AU (1) AU4099893A (en)
SE (1) SE9201615L (en)
WO (1) WO1993023561A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995001452A1 (en) * 1993-06-28 1995-01-12 Akzo Nobel N.V. Quantification of active plasminogen-activator-inhibitor-type-1

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE185376T1 (en) * 1987-07-06 1999-10-15 Biopool Int Inc METHOD FOR MEASURING TISSUE PLASMINOGEN ACTIVATOR, ANTITHROMBIN-III AND SOLUBLE FIBRIN
US5102787A (en) * 1987-07-10 1992-04-07 Miho Sasamata Method, device and kit for measurement of tissue plasminogen activator activity

Also Published As

Publication number Publication date
SE9201615D0 (en) 1992-05-21
AU4099893A (en) 1993-12-13
WO1993023561A1 (en) 1993-11-25

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