SE8803208D0 - IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIALS THEREOF - Google Patents

IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIALS THEREOF

Info

Publication number
SE8803208D0
SE8803208D0 SE8803208A SE8803208A SE8803208D0 SE 8803208 D0 SE8803208 D0 SE 8803208D0 SE 8803208 A SE8803208 A SE 8803208A SE 8803208 A SE8803208 A SE 8803208A SE 8803208 D0 SE8803208 D0 SE 8803208D0
Authority
SE
Sweden
Prior art keywords
cell populations
lymphocyte
hybridomae
vitro
containing cell
Prior art date
Application number
SE8803208A
Other languages
Swedish (sv)
Other versions
SE8803208L (en
SE461985B (en
Inventor
C Glad
C Borrebaeck
Original Assignee
Bioinvent Int Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioinvent Int Ab filed Critical Bioinvent Int Ab
Priority to SE8803208A priority Critical patent/SE461985B/en
Publication of SE8803208D0 publication Critical patent/SE8803208D0/en
Priority to PCT/SE1989/000486 priority patent/WO1990002795A1/en
Priority to AU42069/89A priority patent/AU4206989A/en
Publication of SE8803208L publication Critical patent/SE8803208L/xx
Publication of SE461985B publication Critical patent/SE461985B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method for increasing the yield of antigen-positive hybridomae and the yield of IgG-producing hybridomae amongst antigen-positive hybridomae in in vitro immunisation of lymphocyte-containing cell populations, is described. In the method, lysosomotropic agents are caused to act in vitro on lymphocyte-containing cell populations for the removal of cell populations with a negative effect on the in vitro immunisation, whereupon the lymphocytes are in vitro immunised with thymus-dependent or thymus-independent antigens in the presence of a polyclonal activator. If a well-adjusted quantity of a polyclonal activator is used, it is possible to obtain an increase only in the yield of IgG-producing hybridomae amongst antigen-positive hybridomae in in vitro immunisation without a lysosomotropic agent. A kit for use in in vitro immunisation of lymphocyte-containing cell populations is also described. Said kit comprises at least three containers, of which one holds lymphokines as active constituent, the second holds lysosomotropic agents as active constituent, and the third holds a polyclonal activator as active constituent. Another kit according to the invention does not have the container with lysosomotropic agents.
SE8803208A 1988-09-13 1988-09-13 IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIAL KIT SE461985B (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE8803208A SE461985B (en) 1988-09-13 1988-09-13 IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIAL KIT
PCT/SE1989/000486 WO1990002795A1 (en) 1988-09-13 1989-09-12 In vitro immunisation of lymphocyte-containing cell populations and kit therefor
AU42069/89A AU4206989A (en) 1988-09-13 1989-09-12 In vitro immunisation of lymphocyte-containing cell populations and kit therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8803208A SE461985B (en) 1988-09-13 1988-09-13 IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIAL KIT

Publications (3)

Publication Number Publication Date
SE8803208D0 true SE8803208D0 (en) 1988-09-13
SE8803208L SE8803208L (en) 1990-03-14
SE461985B SE461985B (en) 1990-04-23

Family

ID=20373301

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8803208A SE461985B (en) 1988-09-13 1988-09-13 IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIAL KIT

Country Status (3)

Country Link
AU (1) AU4206989A (en)
SE (1) SE461985B (en)
WO (1) WO1990002795A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU645488B2 (en) * 1989-02-21 1994-01-20 Terumo Corporation A process for the generation of proliferating CD4 lymphocytes
AU657482B2 (en) * 1990-05-22 1995-03-16 Regents Of The University Of California, The Novel methods for producing antigen-specific, high-affinity human monoclonal antibodies
JP3150991B2 (en) * 1991-04-10 2001-03-26 協和醗酵工業株式会社 Hybridoma production method
EA023179B1 (en) 2009-08-13 2016-05-31 Круселл Холланд Б.В. Antibodies against human respiratory syncytial virus (rsv) and methods of use
NZ603488A (en) 2010-07-09 2015-02-27 Crucell Holland Bv Anti-human respiratory syncytial virus (rsv) antibodies and methods of use
US11123372B2 (en) 2016-07-29 2021-09-21 Prokidney Bioactive renal cells for the treatment of chronic kidney disease

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4716111A (en) * 1982-08-11 1987-12-29 Trustees Of Boston University Process for producing human antibodies
SE459008B (en) * 1986-09-04 1989-05-29 Bioinvent Int Ab MAKE PRODUCING HUMAN MONOCLONAL ANTIBODIES

Also Published As

Publication number Publication date
SE8803208L (en) 1990-03-14
AU4206989A (en) 1990-04-02
WO1990002795A1 (en) 1990-03-22
SE461985B (en) 1990-04-23

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