SE8803208D0 - IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIALS THEREOF - Google Patents
IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIALS THEREOFInfo
- Publication number
- SE8803208D0 SE8803208D0 SE8803208A SE8803208A SE8803208D0 SE 8803208 D0 SE8803208 D0 SE 8803208D0 SE 8803208 A SE8803208 A SE 8803208A SE 8803208 A SE8803208 A SE 8803208A SE 8803208 D0 SE8803208 D0 SE 8803208D0
- Authority
- SE
- Sweden
- Prior art keywords
- cell populations
- lymphocyte
- hybridomae
- vitro
- containing cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
A method for increasing the yield of antigen-positive hybridomae and the yield of IgG-producing hybridomae amongst antigen-positive hybridomae in in vitro immunisation of lymphocyte-containing cell populations, is described. In the method, lysosomotropic agents are caused to act in vitro on lymphocyte-containing cell populations for the removal of cell populations with a negative effect on the in vitro immunisation, whereupon the lymphocytes are in vitro immunised with thymus-dependent or thymus-independent antigens in the presence of a polyclonal activator. If a well-adjusted quantity of a polyclonal activator is used, it is possible to obtain an increase only in the yield of IgG-producing hybridomae amongst antigen-positive hybridomae in in vitro immunisation without a lysosomotropic agent. A kit for use in in vitro immunisation of lymphocyte-containing cell populations is also described. Said kit comprises at least three containers, of which one holds lymphokines as active constituent, the second holds lysosomotropic agents as active constituent, and the third holds a polyclonal activator as active constituent. Another kit according to the invention does not have the container with lysosomotropic agents.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8803208A SE461985B (en) | 1988-09-13 | 1988-09-13 | IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIAL KIT |
PCT/SE1989/000486 WO1990002795A1 (en) | 1988-09-13 | 1989-09-12 | In vitro immunisation of lymphocyte-containing cell populations and kit therefor |
AU42069/89A AU4206989A (en) | 1988-09-13 | 1989-09-12 | In vitro immunisation of lymphocyte-containing cell populations and kit therefor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8803208A SE461985B (en) | 1988-09-13 | 1988-09-13 | IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIAL KIT |
Publications (3)
Publication Number | Publication Date |
---|---|
SE8803208D0 true SE8803208D0 (en) | 1988-09-13 |
SE8803208L SE8803208L (en) | 1990-03-14 |
SE461985B SE461985B (en) | 1990-04-23 |
Family
ID=20373301
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8803208A SE461985B (en) | 1988-09-13 | 1988-09-13 | IN VITRO IMMUNIZATION OF Lymphocyte-containing CELL POPULATIONS AND MATERIAL KIT |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU4206989A (en) |
SE (1) | SE461985B (en) |
WO (1) | WO1990002795A1 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU645488B2 (en) * | 1989-02-21 | 1994-01-20 | Terumo Corporation | A process for the generation of proliferating CD4 lymphocytes |
AU657482B2 (en) * | 1990-05-22 | 1995-03-16 | Regents Of The University Of California, The | Novel methods for producing antigen-specific, high-affinity human monoclonal antibodies |
JP3150991B2 (en) * | 1991-04-10 | 2001-03-26 | 協和醗酵工業株式会社 | Hybridoma production method |
EA023179B1 (en) | 2009-08-13 | 2016-05-31 | Круселл Холланд Б.В. | Antibodies against human respiratory syncytial virus (rsv) and methods of use |
NZ603488A (en) | 2010-07-09 | 2015-02-27 | Crucell Holland Bv | Anti-human respiratory syncytial virus (rsv) antibodies and methods of use |
US11123372B2 (en) | 2016-07-29 | 2021-09-21 | Prokidney | Bioactive renal cells for the treatment of chronic kidney disease |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4716111A (en) * | 1982-08-11 | 1987-12-29 | Trustees Of Boston University | Process for producing human antibodies |
SE459008B (en) * | 1986-09-04 | 1989-05-29 | Bioinvent Int Ab | MAKE PRODUCING HUMAN MONOCLONAL ANTIBODIES |
-
1988
- 1988-09-13 SE SE8803208A patent/SE461985B/en not_active IP Right Cessation
-
1989
- 1989-09-12 WO PCT/SE1989/000486 patent/WO1990002795A1/en unknown
- 1989-09-12 AU AU42069/89A patent/AU4206989A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
SE8803208L (en) | 1990-03-14 |
AU4206989A (en) | 1990-04-02 |
WO1990002795A1 (en) | 1990-03-22 |
SE461985B (en) | 1990-04-23 |
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