SE8600011D0 - METHOD OF IDENTIFICATION OF NUCLEIC ACIDS - Google Patents
METHOD OF IDENTIFICATION OF NUCLEIC ACIDSInfo
- Publication number
- SE8600011D0 SE8600011D0 SE8600011A SE8600011A SE8600011D0 SE 8600011 D0 SE8600011 D0 SE 8600011D0 SE 8600011 A SE8600011 A SE 8600011A SE 8600011 A SE8600011 A SE 8600011A SE 8600011 D0 SE8600011 D0 SE 8600011D0
- Authority
- SE
- Sweden
- Prior art keywords
- probe
- identification
- nucleic acids
- hybridization
- biotin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Abstract
A hybridization method for the identification of nucleic acids in solution is presented. At least two probes, a detector probe and a capturing probe are used in the method. The detector probe is labelled with a detectable label and to the capturing probe a member of an affinity pair is attached. The capturing probe: target nucleic acid: detector probe hybrid formed in the hybridization reaction is isolated by the aid of the other member of the affinity pair. Suitable affinity pairs are biotin-avidin or biotin-streptavidin homopolynucleotides, antigens-antibodies etc. The method is faster to perform than the traditional sandwich hybridization.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI850004A FI72146C (en) | 1985-01-02 | 1985-01-02 | Procedure for Identifying Nucleic Acids. |
Publications (3)
Publication Number | Publication Date |
---|---|
SE8600011D0 true SE8600011D0 (en) | 1986-01-02 |
SE8600011L SE8600011L (en) | 1986-07-03 |
SE463212B SE463212B (en) | 1990-10-22 |
Family
ID=8520136
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SE8600011A SE463212B (en) | 1985-01-02 | 1986-01-02 | METHOD FOR IDENTIFYING NUCLEIC ACIDS |
Country Status (21)
Country | Link |
---|---|
JP (1) | JPH0669400B2 (en) |
AT (1) | AT397514B (en) |
AU (1) | AU561382B2 (en) |
BE (1) | BE903937A (en) |
CA (1) | CA1271705A (en) |
CH (1) | CH666696A5 (en) |
DE (1) | DE3546312A1 (en) |
DK (1) | DK164932C (en) |
FI (1) | FI72146C (en) |
FR (1) | FR2575493B1 (en) |
GB (1) | GB2169403B (en) |
HU (1) | HU196453B (en) |
IE (1) | IE58290B1 (en) |
IL (1) | IL77489A (en) |
IT (1) | IT1201514B (en) |
LU (1) | LU86238A1 (en) |
NL (1) | NL189427C (en) |
NO (1) | NO166743C (en) |
RO (1) | RO94651B (en) |
SE (1) | SE463212B (en) |
ZA (1) | ZA859895B (en) |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5288609A (en) * | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
US6060237A (en) | 1985-02-26 | 2000-05-09 | Biostar, Inc. | Devices and methods for optical detection of nucleic acid hybridization |
ES8707343A1 (en) * | 1985-06-13 | 1987-07-16 | Amgen | Method for performing nucleic acid hybridization assays. |
US4910300A (en) * | 1985-12-11 | 1990-03-20 | Chiron Corporation | Method for making nucleic acid probes |
US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US4882269A (en) * | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
FI76119C (en) * | 1986-02-27 | 1988-09-09 | Orion Yhtymae Oy | Quantitative determination of nucleic acid molecules and reagent packaging used in the process |
AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
AU2084988A (en) * | 1987-06-26 | 1989-01-19 | E.I. Du Pont De Nemours And Company | Affinity removal of contaminating sequences from recombinant cloned na using capture beads |
ATE112804T1 (en) * | 1987-07-31 | 1994-10-15 | Gen Probe Inc | POLYNUCLEOTIDE ASSAY USING OLIGONUCLEOTIDES TO ELIMINATE UNDESIRABLE CROSS-REACTIONS. |
EP0305145A3 (en) * | 1987-08-24 | 1990-05-02 | Ortho Diagnostic Systems Inc. | Methods and probes for detecting nucleic acids |
EP0304845A3 (en) * | 1987-08-28 | 1991-03-06 | Profile Diagnostic Sciences Inc. | Method and kit for assaying gene expressions |
CA1325761C (en) * | 1987-12-25 | 1994-01-04 | Takanori Oka | Method of detecting an intended nucleic acid in a sample |
DE3800644A1 (en) * | 1988-01-12 | 1989-07-20 | Boehringer Mannheim Gmbh | PROCESS FOR HIGHLY SPECIFIC DETECTION OF NUCLEIC ACIDS IN SOLUTION |
US5104791A (en) * | 1988-02-09 | 1992-04-14 | E. I. Du Pont De Nemours And Company | Particle counting nucleic acid hybridization assays |
JPH03504199A (en) * | 1988-05-10 | 1991-09-19 | イー・アイ・デユポン・ド・ネモアース・アンド・コンパニー | Rapid nucleic acid detection method |
US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
AU629845B2 (en) * | 1988-08-30 | 1992-10-15 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
US5858652A (en) * | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
GB2225112A (en) * | 1988-11-22 | 1990-05-23 | Ici Plc | Hybridisation probes |
US5082935A (en) * | 1988-12-15 | 1992-01-21 | Amoco Corporation | Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination |
JP3015462B2 (en) * | 1989-02-06 | 2000-03-06 | ジーン‐トラック・システムス | Probes and methods for detecting Listeria |
JP3046837B2 (en) | 1989-03-10 | 2000-05-29 | バイシス・インコーポレーテツド | Immobilized oligonucleotide probes and their use |
JP3046838B2 (en) * | 1989-03-10 | 2000-05-29 | バイシス・インコーポレーテツド | Prevent interference of the affinity capture method |
WO1991000926A1 (en) * | 1989-07-11 | 1991-01-24 | Microprobe Corporation | Quantification of bacteria using a nucleic acid hybridization assay |
US5334501A (en) * | 1989-07-11 | 1994-08-02 | Microprobe Corporation | Quantification of bacteria using a nucleic acid hybridization assay |
GB8924989D0 (en) * | 1989-11-06 | 1989-12-28 | Scotgen Ltd | Method and device for the detection of antibiotic resistance |
US5580970A (en) * | 1989-12-01 | 1996-12-03 | Amoco Corporation | Detection of HPV transcripts |
AU7429591A (en) * | 1990-04-18 | 1991-10-24 | Gene-Trak Systems | Nucleic acid probes for the detection of giardia lamblia |
WO1992015708A1 (en) * | 1991-02-27 | 1992-09-17 | Amoco Corporation | Methods for improving the sensitivity of hybridization assays |
WO1993020234A1 (en) * | 1992-03-31 | 1993-10-14 | E.I. Du Pont De Nemours And Company | A rapid, high capacity nucleic acid based assay |
US7713528B1 (en) | 1993-02-18 | 2010-05-11 | Enzo Therapeutics, Inc. | Method for in vivo delivery of active compounds using reagent conjugate |
US5853993A (en) * | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
GB0016833D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (2) |
US7465540B2 (en) | 2000-09-21 | 2008-12-16 | Luminex Corporation | Multiple reporter read-out for bioassays |
CN1303221C (en) * | 2003-01-27 | 2007-03-07 | 英科新创(厦门)科技有限公司 | Method for detecting target nucleic acid using affinity amplifying integrated signal group |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI63596C (en) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | MICROBIA DIAGNOSIS FOERFARANDE SOM GRUNDAR SIG PAO SKIKTSHYBRIDISERING AV NUCLEINSYROR OCH VID FOERFARANDET ANVAENDA KOMBINATIONER AV REAGENSER |
FR2518755B1 (en) * | 1981-12-23 | 1986-04-11 | Pasteur Institut | PROBE CONTAINING MODIFIED NUCLEIC ACID RECOGNIZABLE BY SPECIFIC ANTIBODIES AND USE OF THE SAME TO DETECT AND CHARACTERIZE A HOMOLOGATED DNA SEQUENCE |
GB8306426D0 (en) * | 1983-03-09 | 1983-04-13 | Malcolm A D B | Detecting polynucleotide sequence |
CA1228811A (en) * | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Assay method utilizing polynucleotide sequences |
NO843838L (en) * | 1983-09-26 | 1985-03-27 | Ortho Diagnostic Systems Inc | PROCEDURE FOR DETECTING NUCLEIC ACID |
ZA849594B (en) * | 1983-12-12 | 1985-08-28 | Miles Lab | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
FR2558172B1 (en) * | 1984-01-16 | 1986-06-13 | Inst Nat Sante Rech Med | PROBE CONTAINING MODIFIED NUCLEIC ACID AND RECOGNITION BY SPECIFIC ANTIBODIES AND USE OF SUCH PROBE AND THESE SPECIFIC ANTIBODIES TO DETECT AND CHARACTERIZE A HOMOLOGATED DNA SEQUENCE |
CA1223222A (en) * | 1984-02-22 | 1987-06-23 | Nanibhushan Dattagupta | Immobilized nucleic acid-containing probes |
FR2560298B1 (en) * | 1984-02-28 | 1988-07-15 | Mors | METHOD AND DEVICE FOR ADJUSTING THE PRECISION OF THE PRESSURE DROP OF A FLUID INITIALLY AT HIGH PRESSURE, SUCH AS A HYDRAULIC FLUID FOR EXAMPLE SUPPLYING A HYDRAULIC GROUP, AND APPLICATION OF THE METHOD TO THE DEVICE IN A HYDRAULIC GROUP OR PLANT INTEGRATED OR NOT, ESPECIALLY IN A MACHINE TABLE OR ANY TOOL SUPPORT DEVICE |
NZ211453A (en) * | 1984-03-22 | 1989-01-06 | Biotechnology Research Enterpr | Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides |
US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
-
1985
- 1985-01-02 FI FI850004A patent/FI72146C/en not_active IP Right Cessation
- 1985-12-17 CH CH5378/85A patent/CH666696A5/en not_active IP Right Cessation
- 1985-12-20 GB GB08531414A patent/GB2169403B/en not_active Expired
- 1985-12-24 BE BE0/216059A patent/BE903937A/en not_active IP Right Cessation
- 1985-12-27 JP JP60299797A patent/JPH0669400B2/en not_active Expired - Lifetime
- 1985-12-27 NO NO855308A patent/NO166743C/en not_active IP Right Cessation
- 1985-12-29 HU HU855030A patent/HU196453B/en unknown
- 1985-12-30 LU LU86238A patent/LU86238A1/en unknown
- 1985-12-30 RO RO121637A patent/RO94651B/en unknown
- 1985-12-30 AT AT0376785A patent/AT397514B/en not_active IP Right Cessation
- 1985-12-30 DE DE19853546312 patent/DE3546312A1/en active Granted
- 1985-12-30 FR FR858519394A patent/FR2575493B1/en not_active Expired - Lifetime
- 1985-12-30 ZA ZA859895A patent/ZA859895B/en unknown
- 1985-12-30 IT IT23408/85A patent/IT1201514B/en active
- 1985-12-31 AU AU51748/85A patent/AU561382B2/en not_active Expired
- 1985-12-31 IE IE333385A patent/IE58290B1/en not_active IP Right Cessation
- 1985-12-31 IL IL77489A patent/IL77489A/en not_active IP Right Cessation
- 1985-12-31 CA CA000498834A patent/CA1271705A/en not_active Expired - Lifetime
- 1985-12-31 NL NLAANVRAGE8503597,A patent/NL189427C/en not_active IP Right Cessation
-
1986
- 1986-01-02 SE SE8600011A patent/SE463212B/en not_active IP Right Cessation
- 1986-01-02 DK DK000386A patent/DK164932C/en not_active IP Right Cessation
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