SA111320572B1 - طريقة تحديد ترتيب pcr معتمدة على تقنية الوسم الجزيئي لحمض dna وإستراتيجية قص غير كامل لحمض dna - Google Patents
طريقة تحديد ترتيب pcr معتمدة على تقنية الوسم الجزيئي لحمض dna وإستراتيجية قص غير كامل لحمض dna Download PDFInfo
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- SA111320572B1 SA111320572B1 SA111320572A SA111320572A SA111320572B1 SA 111320572 B1 SA111320572 B1 SA 111320572B1 SA 111320572 A SA111320572 A SA 111320572A SA 111320572 A SA111320572 A SA 111320572A SA 111320572 B1 SA111320572 B1 SA 111320572B1
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- pcr
- primer
- pairs
- dna
- hla
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201010213717.6A CN101921840B (zh) | 2010-06-30 | 2010-06-30 | 一种基于dna分子标签技术和dna不完全打断策略的pcr测序方法 |
Publications (1)
Publication Number | Publication Date |
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SA111320572B1 true SA111320572B1 (ar) | 2015-04-29 |
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SA111320572A SA111320572B1 (ar) | 2010-06-30 | 2011-07-02 | طريقة تحديد ترتيب pcr معتمدة على تقنية الوسم الجزيئي لحمض dna وإستراتيجية قص غير كامل لحمض dna |
Country Status (3)
Country | Link |
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CN (1) | CN101921840B (zh) |
SA (1) | SA111320572B1 (zh) |
WO (1) | WO2012000152A1 (zh) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
CN101921841B (zh) * | 2010-06-30 | 2014-03-12 | 深圳华大基因科技有限公司 | 基于Illumina GA测序技术的HLA基因高分辨率分型方法 |
WO2012083505A1 (zh) * | 2010-12-24 | 2012-06-28 | 深圳华大基因科技有限公司 | Hla-c基因分型的方法及其相关引物 |
EP2599877B1 (en) | 2010-06-30 | 2017-09-27 | BGI Genomics Co., Limited | New pcr sequencing method and use thereof in hla genotyping |
CN103502448B (zh) | 2010-11-12 | 2017-03-29 | Gen9股份有限公司 | 核酸合成的方法和设备 |
US10457935B2 (en) | 2010-11-12 | 2019-10-29 | Gen9, Inc. | Protein arrays and methods of using and making the same |
CN103270170B (zh) * | 2010-12-24 | 2015-07-29 | 深圳华大基因医学有限公司 | Hla-dqb1基因分型的方法及其相关引物 |
CN102653784B (zh) * | 2011-03-03 | 2015-01-21 | 深圳华大基因科技服务有限公司 | 用于多重核酸测序的标签及其使用方法 |
CN102690809B (zh) * | 2011-03-24 | 2013-12-04 | 深圳华大基因科技服务有限公司 | Dna标签及其在构建和测序配对末端标签文库中的应用 |
LT2944693T (lt) | 2011-08-26 | 2019-08-26 | Gen9, Inc. | Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
LT2841601T (lt) * | 2012-04-24 | 2019-07-10 | Gen9, Inc. | Nukleorūgščių rūšiavimo būdai ir multipleksinis preparatyvinis in vitro klonavimas |
EP3483311A1 (en) | 2012-06-25 | 2019-05-15 | Gen9, Inc. | Methods for nucleic acid assembly and high throughput sequencing |
CN102758026B (zh) * | 2012-06-29 | 2014-05-07 | 深圳华大基因科技有限公司 | 基于HiSeq测序技术检测乙型肝炎病毒分型和耐药基因的方法 |
CN103045591B (zh) * | 2013-01-05 | 2014-03-12 | 上海荻硕贝肯生物科技有限公司 | Hla基因特异性pcr扩增引物及hla分型的方法和试剂盒 |
WO2015047220A2 (en) * | 2013-09-24 | 2015-04-02 | Georgetown University | Compositions and methods for single g-level hla typing |
CN103617375B (zh) * | 2013-12-02 | 2017-08-25 | 深圳华大基因健康科技有限公司 | 聚合酶链式反应产物测序分型的方法及*** |
CN111748606A (zh) * | 2014-06-24 | 2020-10-09 | 北京贝瑞和康医学检验实验室有限公司 | 一种快速构建血浆dna测序文库的方法和试剂盒 |
CN104232631B (zh) * | 2014-08-26 | 2017-12-15 | 深圳华大基因股份有限公司 | 标签、标签引物、试剂盒及其用途 |
CN104293941B (zh) * | 2014-09-30 | 2017-01-11 | 天津华大基因科技有限公司 | 构建测序文库的方法及其应用 |
CN104561294B (zh) * | 2014-12-26 | 2018-03-30 | 北京诺禾致源科技股份有限公司 | 基因分型测序文库的构建方法和测序方法 |
CN107858408A (zh) * | 2016-09-19 | 2018-03-30 | 深圳华大基因科技服务有限公司 | 一种基因组二代序列组装方法和*** |
CN106754904B (zh) * | 2016-12-21 | 2019-03-15 | 南京诺唯赞生物科技有限公司 | 一种cDNA的特异性分子标签及其应用 |
CN108728903A (zh) * | 2017-04-21 | 2018-11-02 | 深圳市乐土精准医疗科技有限公司 | 基于高通量测序用于地中海贫血大样本筛查的建库方法 |
CN109801679B (zh) * | 2019-01-15 | 2021-02-02 | 广州柿宝生物科技有限公司 | 一种用于长链分子的数学序列重建方法 |
CN113564228A (zh) * | 2021-09-26 | 2021-10-29 | 天津诺禾致源生物信息科技有限公司 | 样本的自动化处理方法及装置、样本的自动化处理*** |
CN117437978A (zh) * | 2023-12-12 | 2024-01-23 | 北京旌准医疗科技有限公司 | 一种二代测序数据的低频基因突变分析方法、装置及其应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0400584D0 (en) * | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
JP5237099B2 (ja) * | 2005-09-29 | 2013-07-17 | キージーン ナムローゼ フェンノートシャップ | 変異させた集団のハイスループットスクリーニング |
WO2008045575A2 (en) * | 2006-10-13 | 2008-04-17 | J. Craig Venter Institute, Inc. | Sequencing method |
EP2121983A2 (en) * | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
WO2009049889A1 (en) * | 2007-10-16 | 2009-04-23 | Roche Diagnostics Gmbh | High resolution, high throughput hla genotyping by clonal sequencing |
CN101434988B (zh) * | 2007-11-16 | 2013-05-01 | 深圳华因康基因科技有限公司 | 一种高通量寡核苷酸测序方法 |
CN101654691B (zh) * | 2009-09-23 | 2013-12-04 | 深圳华大基因健康科技有限公司 | Hla基因扩增和基因分型方法及其相关引物 |
-
2010
- 2010-06-30 CN CN201010213717.6A patent/CN101921840B/zh active Active
- 2010-11-15 WO PCT/CN2010/001834 patent/WO2012000152A1/zh active Application Filing
-
2011
- 2011-07-02 SA SA111320572A patent/SA111320572B1/ar unknown
Also Published As
Publication number | Publication date |
---|---|
CN101921840B (zh) | 2014-06-25 |
CN101921840A (zh) | 2010-12-22 |
WO2012000152A1 (zh) | 2012-01-05 |
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