RU98100256A - Способ лечения с применением дозировки лекарственных препаратов и генов, регулируемой посредством электропорации - Google Patents

Способ лечения с применением дозировки лекарственных препаратов и генов, регулируемой посредством электропорации

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Publication number
RU98100256A
RU98100256A RU98100256/14A RU98100256A RU98100256A RU 98100256 A RU98100256 A RU 98100256A RU 98100256/14 A RU98100256/14 A RU 98100256/14A RU 98100256 A RU98100256 A RU 98100256A RU 98100256 A RU98100256 A RU 98100256A
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Prior art keywords
electrodes
tissue
specified
block
needles
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RU98100256/14A
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English (en)
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RU2168337C2 (ru
Inventor
С.Б. Дев
Гантер Э. Хофманн
Ричард Э. Гилберт
Йосухико Хаякава
Ричард Хеллер
Марк Дж. Ярозески
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Дженетроникс, Инк.
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Priority claimed from US08/467,566 external-priority patent/US5702359A/en
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Publication of RU98100256A publication Critical patent/RU98100256A/ru
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Claims (22)

1. Устройство для наложения электрических полей на выбранный участок живого тела, включающее опорные средства (54), блок нескольких электродов (42, 44, 46, 48, 50, 52,), установленных на указанных опорных средствах, причем по меньшей мере несколько электродов имеют форму игл для проникновения в ткань, и электрический импульсный генератор (115) для подачи импульсов электрических сигналов высокой амплитуды на указанные электроды для электропорации клеток, находящихся между указанными электродами, отличающееся тем, что блок электродов состоит из нескольких противоположных пар электродов, установленных на указанных опорных средствах на расстоянии друг от друга, по крайней мере несколько из них имеют форму игл, расположенных на указанных опорных средствах на расстоянии друг от друга для проникновения в ткань по противоположным сторонам выбранной области ткани для in vivo электропорации клеток ткани; и электрический импульсный генератор (115), имеющий средства переключения (104) для избирательной подачи импульсов электрических сигналов высокой амплитуды на выбранные противоположные пары указанных электродов пропорционально расстоянию между указанными противоположными парами электродов для электропорации клеток ткани между указанными электродами.
2. Устройство по п.1, в котором указанные выбранные пары (42, 44, 48, 50, 52) электродов задают углы прямоугольника.
3. Устройство по п.2, в котором указанный блок электродов представляет собой пару пространственно разделенных линейных блоков игольчатых электродов (132, 134), и указанные блоки выполнены с возможностью из регулирования относительно друг друга на указанных опорных средствах.
4. Устройство по п. 2, в котором указанный блок электродов включает кольцевой блок игольчатых электродов (42, 44, 46, 48, 50, 52) и указанные средства переключения (104), обеспечивающие выбранное изменение полярности противоположных пар указанных электродов.
5. Устройство по п.4, в котором указанные средства переключения включают поворотный переключатель (104), избирательно устанавливаемый для поочередного присоединения различных противоположных пар электродов к указанному импульсному генератору.
6. Устройство по пп.1 или 2, в котором все указанные электроды являются игольчатыми электродами (42, 44, 46, 48, 50, 52).
7. Устройство по пп.4 или 5, в котором указанные опорные средства представляют собой втулку (54), установленную на оси (56).
8. Устройство по п.1, в котором по меньшей мере один из указанных игольчатых электродов имеет просвет для инъекции молекул в указанную ткань.
9. Устройство по пп.1, 2, 4 или 5, в котором указанные электроды выполнены с возможностью регулирования относительно указанных опорных средств для регулировки глубины проникновения.
10. Устройство по п.1, в котором указанные электроды включают трубчатые иглы (170, 172) для введения в выбранную ткань и проводники (176, 178), которые могут проходить сквозь указанные иглы в указанную ткань.
11. Устройство по п.10, в котором указанные иглы (170, 172) можно убрать, оставив указанные проводники (176, 178).
12. Устройство по пп.1, 2 или 4, в котором генератор поля (115) генерирует электрическое поле, имеющее силу примерно от 0,2 кВ/см до 20 кВ/см и примерно от одного до тысячи импульсов для наложения на ткань.
13. Применение электропорации для введения молекул в клетки, включающее использование импульсного генератора и блока нескольких электродов, имеющих форму игл для проникновения в ткань, отличающееся тем, что осуществляют: введение указанных игольчатых электродов в выбранную ткань для введения молекул в эту ткань; установку по меньшей мере двух пар электродов указанного блока электродов с обеспечением электропроводности в отношении выбранной ткани, так что указанная ткань находится между первой парой и второй парой электродов; подключение указанного электрического импульсного генератора к указанным электродам; и управление указанным электрическим импульсным генератором для подачи импульсов высокоамплитудных электрических сигналов к противоположным парам электродов пропорционально расстоянию между указанными парами электродов для электропорации клеток ткани.
14. Применение по п.13, в котором указанная операция установки указанного блока электродов заключается в установке пар электродов, задающих углы в прямоугольнике, причем первый и второй игольчатые электроды представляют собой пару трубчатых игл введения в выбранную ткань, и указанные электроды являются разделимыми проводниками, которые можно вводить через указанные иглы в указанную ткань.
15. Применение по п.1, в котором указанные иглы можно удалить, оставив указанные электроды.
16. Применение по п.13, в котором указанная операция использования указанного блока электродов включает использование центрального электрода, обладающего первой полярностью, и нескольких электродов другой полярности, окружающих указанный центральный электрод.
17. Применение по п.16, включающее стадию использования переключающего узла, содержащего поворотный переключатель, избирательно устанавливаемый для поочередного подключения различных противоположных пар электродов к указанному импульсному генератору.
18. Применение по п.13, в котором молекулу выбирают из группы, состоящей из химиотерапевтического агента, полинуклеотида и полипептида.
19. Применение по п.18, в котором химиотерапевтический агент является блеомицином.
20. Применение по п.13, в котором ткань выбирают из группы, состоящей из поджелудочной железы, легкого, сердца, почки, мышцы, молочной железы, прямой кишки, простаты, тимуса, семенника и яичника.
21. Применение по п.13, в котором указанная операция использования указанного блока электродов включает использование блока нескольких игольчатых электродов, состоящих из нескольких противоположных пар игольчатых электродов для введения в выбранную ткань, и указанная операция подачи импульсов на указанные электроды включает выборочную подачу импульсов на противоположные пары электродов.
22. Применение по п.21, в котором операция подачи импульсов на указанный блок электродов включает подачу указанных импульсов последовательно к парам электродов в указанном блоке.
RU98100256/14A 1995-06-06 1996-05-22 Способ лечения с введением лекарственных препаратов и генов посредством электропорации RU2168337C2 (ru)

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Application Number Priority Date Filing Date Title
US08/467,566 1995-06-06
US08/467,566 US5702359A (en) 1995-06-06 1995-06-06 Needle electrodes for mediated delivery of drugs and genes
US08/537,265 1995-09-29

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RU2168337C2 RU2168337C2 (ru) 2001-06-10

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Families Citing this family (246)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5702359A (en) * 1995-06-06 1997-12-30 Genetronics, Inc. Needle electrodes for mediated delivery of drugs and genes
US5993434A (en) * 1993-04-01 1999-11-30 Genetronics, Inc. Method of treatment using electroporation mediated delivery of drugs and genes
IL108775A (en) * 1994-02-25 2003-09-17 Univ Ramot Method for efficient incorporation of molecules into cells
US6322548B1 (en) 1995-05-10 2001-11-27 Eclipse Surgical Technologies Delivery catheter system for heart chamber
US6041252A (en) * 1995-06-07 2000-03-21 Ichor Medical Systems Inc. Drug delivery system and method
JP4175662B2 (ja) 1996-01-08 2008-11-05 インパルス ダイナミクス エヌ．ヴイ． 電気的筋肉制御装置
US9289618B1 (en) 1996-01-08 2016-03-22 Impulse Dynamics Nv Electrical muscle controller
US8825152B2 (en) 1996-01-08 2014-09-02 Impulse Dynamics, N.V. Modulation of intracellular calcium concentration using non-excitatory electrical signals applied to the tissue
US8321013B2 (en) 1996-01-08 2012-11-27 Impulse Dynamics, N.V. Electrical muscle controller and pacing with hemodynamic enhancement
US7167748B2 (en) 1996-01-08 2007-01-23 Impulse Dynamics Nv Electrical muscle controller
US9713723B2 (en) 1996-01-11 2017-07-25 Impulse Dynamics Nv Signal delivery through the right ventricular septum
CA2253549C (en) * 1996-06-18 2005-10-25 Alza Corporation Device for enhancing transdermal agent delivery or sampling
WO1998011937A1 (en) * 1996-09-17 1998-03-26 Deka Products Limited Partnership System for delivery of drugs by transport
US6261281B1 (en) * 1997-04-03 2001-07-17 Electrofect As Method for genetic immunization and introduction of molecules into skeletal muscle and immune cells
EA002087B1 (ru) * 1997-04-03 2001-12-24 Электрофект Ас Способ введения фармацевтических препаратов и нуклеиновых кислот в скелетную мышцу
US5873849A (en) * 1997-04-24 1999-02-23 Ichor Medical Systems, Inc. Electrodes and electrode arrays for generating electroporation inducing electrical fields
BR9810372A (pt) 1997-06-30 2000-09-05 Rhone Poulenc Rorer Sa Processo de transferência de ácido nucléico para o interior de células de organismos eucarióticos pluricelulares in vivo, composição, ácido nucléico e campo elétrico, e, produto de combinação
WO1999001158A1 (fr) 1997-06-30 1999-01-14 Rhone-Poulenc Rorer S.A. Amelioration du transfert d'acide nucleique dans le muscle strie et combinaison permettant la mise en oeuvre du procede
ES2283020T3 (es) 1997-07-16 2007-10-16 Metacure Nv Controlador del musculo liso.
US6096037A (en) 1997-07-29 2000-08-01 Medtronic, Inc. Tissue sealing electrosurgery device and methods of sealing tissue
US6241701B1 (en) 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
US6055453A (en) * 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
US6216034B1 (en) 1997-08-01 2001-04-10 Genetronics, Inc. Method of programming an array of needle electrodes for electroporation therapy of tissue
SE9704076D0 (sv) 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
US20050026283A1 (en) * 2001-11-27 2005-02-03 Owe Ormar Method for combined parallel agent delivery and electroporation for cell structures and thereof
US6356783B1 (en) 1997-11-20 2002-03-12 David R. Hubbard, Jr. Multi-electrode and needle injection device for diagnosis and treatment of muscle injury and pain
AU733053C (en) 1998-02-24 2001-11-29 Boston Scientific Limited High flow rate dialysis catheters and related methods
US6027488A (en) 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
US20040229363A1 (en) * 1998-06-24 2004-11-18 Ed Nolan High efficiency transfection based on low electric field strength, long pulse length
AU760619B2 (en) * 1998-06-26 2003-05-22 Genetronics, Inc. Synergism of photodynamic and electropermeation effects on cell vitality as a novel cytotoxic agent
AU770092B2 (en) * 1998-07-13 2004-02-12 Genetronics, Inc. Skin and muscle-targeted gene therapy by pulsed electrical field
US7922709B2 (en) 1998-07-13 2011-04-12 Genetronics, Inc. Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation
US6678556B1 (en) 1998-07-13 2004-01-13 Genetronics, Inc. Electrical field therapy with reduced histopathological change in muscle
JP2002520101A (ja) 1998-07-13 2002-07-09 ジェネトロニクス、インコーポレーテッド 電気的に補助される化粧用薬剤の局部送達法および装置
CA2338280A1 (en) 1998-07-20 2000-02-03 Andrew W. Hannaman Electroporation electrodes
US6319901B1 (en) 1998-10-15 2001-11-20 Ichor Medical Systems, Inc. Methods for prolonging cell membrane permeability
US8700161B2 (en) 1999-03-05 2014-04-15 Metacure Limited Blood glucose level control
US9101765B2 (en) 1999-03-05 2015-08-11 Metacure Limited Non-immediate effects of therapy
US8666495B2 (en) 1999-03-05 2014-03-04 Metacure Limited Gastrointestinal methods and apparatus for use in treating disorders and controlling blood sugar
EP1171189B1 (en) 1999-03-25 2016-05-11 Inovio Pharmaceuticals, Inc. Apparatus for reducing electroporation-mediated muscle reaction and pain response
US6678558B1 (en) * 1999-03-25 2004-01-13 Genetronics, Inc. Method and apparatus for reducing electroporation-mediated muscle reaction and pain response
US7171264B1 (en) * 1999-05-10 2007-01-30 Genetronics, Inc. Intradermal delivery of active agents by needle-free injection and electroporation
WO2000067837A1 (en) 1999-05-10 2000-11-16 Gentronics, Inc. Method of electroporation-enhanced delivery of active agents
US7190997B1 (en) * 1999-06-04 2007-03-13 Impulse Dynamics Nv Drug delivery device
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US6326177B1 (en) 1999-08-04 2001-12-04 Eastern Virginia Medical School Of The Medical College Of Hampton Roads Method and apparatus for intracellular electro-manipulation
US6549810B1 (en) 1999-12-01 2003-04-15 Vertis Neuroscience, Inc. Percutaneous electrical therapy system with electrode depth control
US6516226B1 (en) 1999-12-01 2003-02-04 Vertis Neuroscience, Inc. Percutaneous electrical therapy system for minimizing electrode insertion discomfort
US6493592B1 (en) 1999-12-01 2002-12-10 Vertis Neuroscience, Inc. Percutaneous electrical therapy system with electrode position maintenance
US6622051B1 (en) 1999-12-01 2003-09-16 Vertis Neuroscience, Inc. Percutaneous electrical therapy system with electrode entry angle control
US6539264B1 (en) 1999-12-01 2003-03-25 Vertis Neuroscience, Inc. Percutaneous electrical therapy system with sharp point protection
US6522927B1 (en) 1999-12-01 2003-02-18 Vertis Neuroscience, Inc. Electrode assembly for a percutaneous electrical therapy system
US6912424B2 (en) * 1999-12-01 2005-06-28 Meagan, Medical, Inc. Apparatus and method for coupling therapeutic and/or monitoring equipment to a patient
US6549797B1 (en) 1999-12-01 2003-04-15 Vertis Neuroscience, Inc. Electrode remover for a percutaneous electrical therapy system
US6542780B1 (en) * 1999-12-01 2003-04-01 Vertis Neuroscience, Inc. Method and apparatus for electrically coupling a percutaneous probe
US6560491B1 (en) 1999-12-01 2003-05-06 Vertis Neuroscience, Inc. Percutaneous electrical therapy system providing electrode axial support
US6904324B2 (en) * 1999-12-01 2005-06-07 Meagan Medical, Inc. Method and apparatus for deploying a percutaneous probe
US6556869B1 (en) 1999-12-01 2003-04-29 Vertis Neuroscience, Inc. Electrode introducer for a percutaneous electrical therapy system
CA2394020C (en) 1999-12-15 2011-04-05 University Of South Florida Electroporation device and method
US20020107514A1 (en) 2000-04-27 2002-08-08 Hooven Michael D. Transmural ablation device with parallel jaws
US6932811B2 (en) 2000-04-27 2005-08-23 Atricure, Inc. Transmural ablation device with integral EKG sensor
US6905498B2 (en) 2000-04-27 2005-06-14 Atricure Inc. Transmural ablation device with EKG sensor and pacing electrode
US6546935B2 (en) 2000-04-27 2003-04-15 Atricure, Inc. Method for transmural ablation
WO2001089455A2 (en) 2000-05-22 2001-11-29 Merck & Company, Inc. System and method for assessing the performance of a pharmaceutical agent delivery system
WO2002000278A2 (en) * 2000-06-26 2002-01-03 Microheart, Inc. Method and apparatus for treating ischemic tissue
US6892099B2 (en) 2001-02-08 2005-05-10 Minnesota Medical Physics, Llc Apparatus and method for reducing subcutaneous fat deposits, virtual face lift and body sculpturing by electroporation
US6795728B2 (en) 2001-08-17 2004-09-21 Minnesota Medical Physics, Llc Apparatus and method for reducing subcutaneous fat deposits by electroporation
US8251986B2 (en) 2000-08-17 2012-08-28 Angiodynamics, Inc. Method of destroying tissue cells by eletroporation
US6697670B2 (en) 2001-08-17 2004-02-24 Minnesota Medical Physics, Llc Apparatus and method for reducing subcutaneous fat deposits by electroporation with improved comfort of patients
US7118555B2 (en) * 2000-09-21 2006-10-10 Meagan Medical, Inc. Method and apparatus for repositioning a percutaneous probe
US6671557B1 (en) 2000-10-10 2003-12-30 Meagan Medical, Inc. System and method for providing percutaneous electrical therapy
US6701190B2 (en) 2000-10-10 2004-03-02 Meagan Medical, Inc. System and method for varying characteristics of electrical therapy
US7740623B2 (en) 2001-01-13 2010-06-22 Medtronic, Inc. Devices and methods for interstitial injection of biologic agents into tissue
US20040138621A1 (en) 2003-01-14 2004-07-15 Jahns Scott E. Devices and methods for interstitial injection of biologic agents into tissue
CA2478614C (en) * 2001-03-13 2013-01-15 University Of South Florida Electromanipulation device and method
US6625486B2 (en) 2001-04-11 2003-09-23 Advanced Cardiovascular Systems, Inc. Method and apparatus for intracellular delivery of an agent
US7127284B2 (en) * 2001-06-11 2006-10-24 Mercator Medsystems, Inc. Electroporation microneedle and methods for its use
US20040204669A1 (en) * 2001-07-05 2004-10-14 Hofmann Gunter A. Apparatus for electroporation mediated delivery for drugs and genes
USRE42016E1 (en) 2001-08-13 2010-12-28 Angiodynamics, Inc. Apparatus and method for the treatment of benign prostatic hyperplasia
US6994706B2 (en) 2001-08-13 2006-02-07 Minnesota Medical Physics, Llc Apparatus and method for treatment of benign prostatic hyperplasia
WO2003047684A2 (en) * 2001-12-04 2003-06-12 University Of Southern California Method for intracellular modifications within living cells using pulsed electric fields
US7967816B2 (en) 2002-01-25 2011-06-28 Medtronic, Inc. Fluid-assisted electrosurgical instrument with shapeable electrode
CA2476452A1 (en) * 2002-02-15 2003-08-28 Zycos Inc. Electroporation methods for introducing bioactive agents into cells
EP1480692A2 (en) 2002-03-07 2004-12-01 Merck & Co., Inc. Clinical syringe with electrical stimulation aspects
US7245963B2 (en) * 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
CA2995944C (en) * 2002-03-07 2023-09-12 Advisys, Inc. Electrode system for facilitating macromolecule introduction
US8209006B2 (en) * 2002-03-07 2012-06-26 Vgx Pharmaceuticals, Inc. Constant current electroporation device and methods of use
US7218962B2 (en) * 2002-03-29 2007-05-15 Boston Scientific Scimed, Inc. Magnetically enhanced injection catheter
US6912417B1 (en) 2002-04-05 2005-06-28 Ichor Medical Systmes, Inc. Method and apparatus for delivery of therapeutic agents
ATE402732T1 (de) 2002-04-16 2008-08-15 Cyto Pulse Sciences Inc Verfahren zur behandlung von biologischen materialien mit übersetzenden elektrischen feldern und elektroden-polaritäts-umkehr
US7328064B2 (en) 2002-07-04 2008-02-05 Inovio As Electroporation device and injection apparatus
US20040078063A1 (en) * 2002-10-17 2004-04-22 Mclaren Brian Electrical apparatus and methods for denaturing venoms and toxins
US9931503B2 (en) 2003-03-10 2018-04-03 Impulse Dynamics Nv Protein activity modification
US11439815B2 (en) 2003-03-10 2022-09-13 Impulse Dynamics Nv Protein activity modification
CN1787850B (zh) 2003-03-10 2015-12-16 脉冲动力公司 用于传送电信号以修改心脏组织中基因表达的装置与方法
US7288092B2 (en) 2003-04-23 2007-10-30 Atricure, Inc. Method and apparatus for ablating cardiac tissue with guide facility
US8792985B2 (en) 2003-07-21 2014-07-29 Metacure Limited Gastrointestinal methods and apparatus for use in treating disorders and controlling blood sugar
US8298222B2 (en) 2003-12-24 2012-10-30 The Regents Of The University Of California Electroporation to deliver chemotherapeutics and enhance tumor regression
US20050171574A1 (en) 2003-12-24 2005-08-04 The Regents Of The University Of California Electroporation to interrupt blood flow
WO2005065722A2 (en) * 2003-12-31 2005-07-21 Advisys, Inc. Reducing arthritis and lameness in subjects by growth hormone releasing hormone (ghrh) supplementation
EP1713828B1 (en) * 2004-01-20 2011-04-27 VGX Pharmaceuticals, LLC Enhanced secretion/retention of growth hormone releasing hormone (ghrh) from muscle cells by species-specific signal peptide
GB0402569D0 (en) * 2004-02-05 2004-03-10 Neurodan As Nerve and/or muscle stimulation electrodes
US9364664B2 (en) 2004-03-08 2016-06-14 Ichor Medical Systems, Inc. Apparatus for electrically mediated delivery of therapeutic agents
US11779768B2 (en) 2004-03-10 2023-10-10 Impulse Dynamics Nv Protein activity modification
US8548583B2 (en) 2004-03-10 2013-10-01 Impulse Dynamics Nv Protein activity modification
US8352031B2 (en) 2004-03-10 2013-01-08 Impulse Dynamics Nv Protein activity modification
JP2005278480A (ja) * 2004-03-29 2005-10-13 Fujitsu Ltd 物質導入装置及び物質導入用チップ
AU2005231443B2 (en) 2004-04-01 2012-02-23 The General Hospital Corporation Method and apparatus for dermatological treatment and tissue reshaping
US20050226991A1 (en) * 2004-04-07 2005-10-13 Hossainy Syed F Methods for modifying balloon of a catheter assembly
US7530980B2 (en) 2004-04-14 2009-05-12 Atricure, Inc Bipolar transmural ablation method and apparatus
JP4504082B2 (ja) * 2004-04-28 2010-07-14 富士通株式会社 液体注入装置
EP1750608B1 (en) 2004-06-02 2012-10-03 Medtronic, Inc. Ablation device with jaws
MX2007000893A (es) * 2004-07-23 2007-04-18 Advisys Inc La hormona liberadora de hormona de crecimiento mejora la respuesta inmune inducida por la vacunacion.
WO2006084173A1 (en) * 2005-02-04 2006-08-10 The Johns Hopkins University Electroporation gene therapy gun system
GB0502384D0 (en) * 2005-02-04 2005-03-16 Instrumedical Ltd Electro-surgical needle apparatus
WO2006097934A2 (en) 2005-03-18 2006-09-21 Metacure Limited Pancreas lead
US8114070B2 (en) * 2005-06-24 2012-02-14 Angiodynamics, Inc. Methods and systems for treating BPH using electroporation
US20060293730A1 (en) 2005-06-24 2006-12-28 Boris Rubinsky Methods and systems for treating restenosis sites using electroporation
US7647122B2 (en) * 2005-07-26 2010-01-12 Medtronic, Inc. Surgical needle driver
CA2631719A1 (en) * 2005-12-07 2007-10-25 Genetronics, Inc. Variable volume electroporation chamber and methods therefore
US20080287857A1 (en) * 2006-02-11 2008-11-20 Rune Kjeken Device and method for single-needle in vivo electroporation
CA3063263C (en) 2006-02-11 2024-01-16 Genetronics, Inc. Device and method for single-needle in vivo electroporation
US20080045880A1 (en) * 2006-02-11 2008-02-21 Rune Kjeken Device and method for single-needle in vivo electroporation
US9037230B2 (en) 2006-03-03 2015-05-19 Oncosec Medical Incorporated Method and device for treating microscopic tumors remaining in tissues following surgical resection
WO2007117651A2 (en) * 2006-04-07 2007-10-18 University Of South Florida Passive electric field focus system for in vivo and in vitro applications
WO2007144004A1 (en) * 2006-06-12 2007-12-21 Region Hovedstaden V/Herlev Hospital Electrode introducer device
US8058253B2 (en) * 2006-07-06 2011-11-15 Vgx Pharmaceuticals, Inc. Growth hormone releasing hormone treatment to decrease cholesterol levels
US8142426B2 (en) 2006-10-16 2012-03-27 Syneron Medical Ltd. Methods and devices for treating tissue
US8133216B2 (en) 2006-10-16 2012-03-13 Syneron Medical Ltd. Methods and devices for treating tissue
EP2076313A4 (en) 2006-10-16 2012-07-25 Univ California PREDETERMINED CONDUCTIVITY GELS FOR IRREVERSIBLE ELECTROPORATION OF FABRICS
US8007493B2 (en) 2006-10-16 2011-08-30 Syneron Medical Ltd. Methods and devices for treating tissue
US8273080B2 (en) 2006-10-16 2012-09-25 Syneron Medical Ltd. Methods and devices for treating tissue
CN105457157B (zh) * 2006-10-17 2020-04-14 因诺维奥制药公司 电穿孔装置及用其进行哺乳动物细胞电穿孔的方法
US20080312647A1 (en) * 2007-06-15 2008-12-18 Primaeva Medical, Inc. Methods and devices for treating tissue
US8845630B2 (en) 2007-06-15 2014-09-30 Syneron Medical Ltd Devices and methods for percutaneous energy delivery
US20090036958A1 (en) * 2007-08-01 2009-02-05 Primaeva Medical, Inc. Methods and devices for treating tissue
JP2011509743A (ja) * 2008-01-17 2011-03-31 ジェネトロニクス，インコーポレイティド 可変電流密度単一針電気穿孔システムおよび方法
US20090248012A1 (en) 2008-03-27 2009-10-01 The Regents Of The University Of California Irreversible electroporation device and method for attenuating neointimal
CN102016023A (zh) 2008-04-04 2011-04-13 宾夕法尼亚州立大学托管会 使用il-28和组合物的疫苗和免疫治疗及其使用方法
KR101589511B1 (ko) * 2008-04-04 2016-02-01 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 치쿤구니야 바이러스 단백질의 공통 서열, 이를 인코딩하는 핵산 분자, 조성물 및 이를 이용하는 방법
US9867652B2 (en) 2008-04-29 2018-01-16 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
US10702326B2 (en) 2011-07-15 2020-07-07 Virginia Tech Intellectual Properties, Inc. Device and method for electroporation based treatment of stenosis of a tubular body part
US9598691B2 (en) 2008-04-29 2017-03-21 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation to create tissue scaffolds
US10245098B2 (en) 2008-04-29 2019-04-02 Virginia Tech Intellectual Properties, Inc. Acute blood-brain barrier disruption using electrical energy based therapy
US10238447B2 (en) 2008-04-29 2019-03-26 Virginia Tech Intellectual Properties, Inc. System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress
US9283051B2 (en) 2008-04-29 2016-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US8992517B2 (en) 2008-04-29 2015-03-31 Virginia Tech Intellectual Properties Inc. Irreversible electroporation to treat aberrant cell masses
US10117707B2 (en) 2008-04-29 2018-11-06 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US10272178B2 (en) 2008-04-29 2019-04-30 Virginia Tech Intellectual Properties Inc. Methods for blood-brain barrier disruption using electrical energy
US11272979B2 (en) 2008-04-29 2022-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
US9198733B2 (en) 2008-04-29 2015-12-01 Virginia Tech Intellectual Properties, Inc. Treatment planning for electroporation-based therapies
US11254926B2 (en) 2008-04-29 2022-02-22 Virginia Tech Intellectual Properties, Inc. Devices and methods for high frequency electroporation
WO2009137609A2 (en) 2008-05-06 2009-11-12 Cellutions, Inc. Apparatus and systems for treating a human tissue condition
WO2009137800A2 (en) 2008-05-09 2009-11-12 Angiodynamics, Inc. Electroporation device and method
US9173704B2 (en) 2008-06-20 2015-11-03 Angiodynamics, Inc. Device and method for the ablation of fibrin sheath formation on a venous catheter
WO2010008834A2 (en) 2008-06-23 2010-01-21 Angiodynamics, Inc. Treatment devices and methods
KR101054828B1 (ko) 2009-01-22 2011-08-05 신경민 고주파 열 치료용 전극 장치
WO2010085765A2 (en) 2009-01-23 2010-07-29 Moshe Meir H Therapeutic energy delivery device with rotational mechanism
US8231603B2 (en) 2009-02-10 2012-07-31 Angiodynamics, Inc. Irreversible electroporation and tissue regeneration
US11638603B2 (en) 2009-04-09 2023-05-02 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US11382681B2 (en) 2009-04-09 2022-07-12 Virginia Tech Intellectual Properties, Inc. Device and methods for delivery of high frequency electrical pulses for non-thermal ablation
WO2010118387A1 (en) 2009-04-09 2010-10-14 Virginia Tech Intellectual Properties, Inc. Integration of very short electric pulses for minimally to noninvasive electroporation
USD630321S1 (en) 2009-05-08 2011-01-04 Angio Dynamics, Inc. Probe handle
WO2010138919A2 (en) 2009-05-28 2010-12-02 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
EP3488837A1 (en) 2009-07-15 2019-05-29 NewSouth Innovations Pty Limited Electroporation apparatus for providing agents to the cochlea
KR20120093163A (ko) 2009-09-14 2012-08-22 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 Ｉｌ-15 수용체 알파 및/또는 이를 인코딩하는 핵산 분자를 포함하는 백신 및 면역 치료제, 및 이를 이용하는 방법
US20110118732A1 (en) 2009-11-19 2011-05-19 The Regents Of The University Of California Controlled irreversible electroporation
WO2011092710A2 (en) 2010-02-01 2011-08-04 Metacure Limited Gastrointestinal electrical therapy
US20120076808A1 (en) 2010-09-27 2012-03-29 China Agricultural University Combined antigen and dna vaccine for preventing and treating autoimmune diseases
US9700368B2 (en) 2010-10-13 2017-07-11 Angiodynamics, Inc. System and method for electrically ablating tissue of a patient
WO2012088149A2 (en) 2010-12-20 2012-06-28 Virginia Tech Intellectual Properties, Inc. High-frequency electroporation for cancer therapy
CN102631243B (zh) * 2011-02-15 2014-09-17 成功大学 电磁热疗法的治疗器具
CA2839196A1 (en) 2011-06-15 2012-12-20 Chrontech Pharma Ab Injection needle and device
JP2014524796A (ja) * 2011-06-28 2014-09-25 イノビオ ファーマシューティカルズ，インコーポレイティド 低侵襲表皮電気穿孔装置
US9078665B2 (en) 2011-09-28 2015-07-14 Angiodynamics, Inc. Multiple treatment zone ablation probe
US10206997B2 (en) 2011-11-10 2019-02-19 Bin Wang Facilitator-DNA combination vaccine
ITCO20120004A1 (it) * 2012-02-07 2013-08-08 Giuseppe Caccia Apparecchio per elettroporazzione dinamica vaginale e anale
US9414881B2 (en) 2012-02-08 2016-08-16 Angiodynamics, Inc. System and method for increasing a target zone for electrical ablation
CN103239734B (zh) 2012-02-10 2016-02-24 北京艾棣维欣生物技术有限公司 用于预防和/或治疗呼吸道合胞病毒感染的疫苗
US9144459B2 (en) 2012-07-19 2015-09-29 Cook Medical Technologies Llc Endoscopic ultrasound ablation needle
US10220082B2 (en) 2012-12-13 2019-03-05 Inovio Pharmaceuticals, Inc. WT1 vaccine
US10392611B2 (en) 2013-05-30 2019-08-27 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
US9592303B2 (en) 2013-05-30 2017-03-14 Duke University Enzyme-catalyzed synthesis of site-specific and stoichiometric biomolecule-polymer conjugates
US10364451B2 (en) 2013-05-30 2019-07-30 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
EP3010582B1 (en) 2013-06-21 2021-04-28 Newsouth Innovations Pty Limited Method and apparatus for close-field electroporation
CA2919835A1 (en) 2013-07-31 2015-02-05 Board Of Trustees Of The University Of Arkansas Compositions for and methods of treating and preventing cancer targeting tumor associated carbohydrate antigens
EP3054972B1 (en) 2013-10-07 2019-02-27 The Trustees Of The University Of Pennsylvania Vaccines with interleukin-33 as an adjuvant
US10370664B2 (en) 2013-11-07 2019-08-06 University Of Southern California Use of IKK epsilon inhibitors to activate NFAT and T cell response
WO2015073291A1 (en) 2013-11-14 2015-05-21 The Trustees Of The University Of Pennsylvania Hiv-1 env dna vaccine plus protein boost
IL305392A (en) 2013-11-29 2023-10-01 Inovio Pharmaceuticals Inc MERS-COV vaccine
CA2935375C (en) 2014-01-06 2023-08-08 The Trustees Of The University Of Pennsylvania Pd1 and pdl1 antibodies and vaccine combinations and use of same for immunotherapy
US10166321B2 (en) 2014-01-09 2019-01-01 Angiodynamics, Inc. High-flow port and infusion needle systems
CN106255525B (zh) * 2014-05-02 2020-02-11 皇家飞利浦有限公司 用于使细菌失活的设备
WO2015175570A1 (en) 2014-05-12 2015-11-19 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
KR20230145241A (ko) 2014-10-01 2023-10-17 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 항원 및 어쥬번트로서 인터류킨-21을 갖는 백신
US10694972B2 (en) 2014-12-15 2020-06-30 Virginia Tech Intellectual Properties, Inc. Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment
CA2974956A1 (en) 2015-01-29 2016-08-04 The Trustees Of The University Of Pennsylvania Checkpoint inhibitor and vaccine combinations and use of same for immunotherapy
US10385115B2 (en) 2015-03-26 2019-08-20 Duke University Fibronectin type III domain-based fusion proteins
KR20180033586A (ko) 2015-08-04 2018-04-03 듀크 유니버시티 전달을 위한 유전적으로 인코딩된 내재적으로 무질서화된 스텔스 중합체 및 이의 이용 방법
US20190015520A1 (en) 2015-12-21 2019-01-17 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
US11752213B2 (en) 2015-12-21 2023-09-12 Duke University Surfaces having reduced non-specific binding and antigenicity
EP3397337B1 (en) 2015-12-30 2020-07-29 Inovio Pharmaceuticals, Inc. Electroporation device with detachable needle array with lock-out system
CA3018223A1 (en) 2016-03-28 2017-10-05 Ichor Medical Systems, Inc. Method and apparatus for delivery of therapeutic agents
WO2017173398A1 (en) 2016-04-01 2017-10-05 Duke University Alpha-helical peptide nanofibers as a self-adjuvanting vaccine platform
AU2017257191B2 (en) 2016-04-29 2023-08-10 Inovio Pharmaceuticals, Inc. The in vivo use of chondroitinase and/or hyaluronidase to enhance delivery of an agent
US20170340283A1 (en) * 2016-05-24 2017-11-30 Ironwood Electronics, Inc. Needle probe array and methods regarding same
US11467156B2 (en) 2016-06-01 2022-10-11 Duke University Nonfouling biosensors
IT201600068691A1 (it) * 2016-07-01 2018-01-01 Igea S P A Sistema di movimentazione e controllo per elettrodi espandibili di un manipolo utilizzabile in un procedimento di elettro-porazione
EP3512868A4 (en) 2016-09-14 2020-04-15 Duke University NANOPARTICLES BASED ON TRIBLOCK POLYPEPTIDE FOR THE DELIVERY OF HYDROPHILIC DRUGS
EP3515928A4 (en) 2016-09-23 2020-04-01 Duke University UNSTRUCTURED NON-REPITITIVE POLYPEPTIDES WITH LCST BEHAVIOR
US10905492B2 (en) 2016-11-17 2021-02-02 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
US10376495B2 (en) 2016-11-23 2019-08-13 University Of South Florida Small molecules that mimic or antagonize actions of granulocyte colony-stimulating-factor (G-CSF)
US11235132B2 (en) 2016-12-22 2022-02-01 Ohio State Innovation Foundation Interpenetrating microstructures for nanochannel-based cargo delivery
US11648200B2 (en) 2017-01-12 2023-05-16 Duke University Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature triggered hierarchical self-assembly
US10813935B2 (en) 2017-02-23 2020-10-27 Transgenex Nanobiotech, Inc. Methods and compositions for treating drug resistance in cancer
US11318155B2 (en) 2017-02-24 2022-05-03 University Of South Florida Hsp90 activator Aha1 drives production of pathological tau aggregates
US10272052B2 (en) 2017-02-24 2019-04-30 University Of South Florida Compositions and methods for the treatment of tauopathies
US11554097B2 (en) 2017-05-15 2023-01-17 Duke University Recombinant production of hybrid lipid-biopolymer materials that self-assemble and encapsulate agents
US11680083B2 (en) 2017-06-30 2023-06-20 Duke University Order and disorder as a design principle for stimuli-responsive biopolymer networks
GB201710973D0 (en) 2017-07-07 2017-08-23 Avacta Life Sciences Ltd Scaffold proteins
US10850095B2 (en) 2017-08-08 2020-12-01 Pulse Biosciences, Inc. Treatment of tissue by the application of energy
US11590345B2 (en) 2017-08-08 2023-02-28 Pulse Biosciences, Inc. Treatment of tissue by the application of energy
US10857347B2 (en) 2017-09-19 2020-12-08 Pulse Biosciences, Inc. Treatment instrument and high-voltage connectors for robotic surgical system
US11607537B2 (en) 2017-12-05 2023-03-21 Virginia Tech Intellectual Properties, Inc. Method for treating neurological disorders, including tumors, with electroporation
EP3752136A1 (en) 2018-02-13 2020-12-23 Purdue Research Foundation Inactivating bacteria with electric pulses and antibiotics
US11311329B2 (en) 2018-03-13 2022-04-26 Virginia Tech Intellectual Properties, Inc. Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
US11925405B2 (en) 2018-03-13 2024-03-12 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
EP3829622A4 (en) 2018-08-02 2022-05-11 Duke University DUAL AGONIST FUSION PROTEINS
US11571569B2 (en) 2019-02-15 2023-02-07 Pulse Biosciences, Inc. High-voltage catheters for sub-microsecond pulsing
US11950835B2 (en) 2019-06-28 2024-04-09 Virginia Tech Intellectual Properties, Inc. Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy
BR102019013578A2 (pt) * 2019-06-28 2021-01-05 Eqt Equipamentos E Tecnologia Ltda. Ponta aplicadora de eletroporação para procedimento de eletroquimioterapia
US11512314B2 (en) 2019-07-12 2022-11-29 Duke University Amphiphilic polynucleotides
US11912975B2 (en) 2019-09-02 2024-02-27 Mirai Medical Limited Electroporation apparatus and method
TW202128775A (zh) 2019-10-16 2021-08-01 英商阿法克塔生命科學有限公司 PD-L1抑制劑-TGFβ抑制劑雙特異性藥物部分
US11660335B2 (en) 2020-02-25 2023-05-30 Inovio Pharmaceuticals, Inc. Vaccines against coronavirus and methods of use
PE20230349A1 (es) 2020-05-14 2023-03-02 Inovio Pharmaceuticals Inc Vacunas para la papilomatosis respiratoria recurrente y metodos para usar estas
GB202101299D0 (en) 2020-06-09 2021-03-17 Avacta Life Sciences Ltd Diagnostic polypetides and methods
IL298874A (en) 2020-06-12 2023-02-01 Univ Rochester Encoding and expression of ACE-tRNAs
WO2022234003A1 (en) 2021-05-07 2022-11-10 Avacta Life Sciences Limited Cd33 binding polypeptides with stefin a protein
TW202334196A (zh) 2021-10-07 2023-09-01 英商阿法克塔生命科學有限公司 Pd-l1結合多肽
WO2023057946A1 (en) 2021-10-07 2023-04-13 Avacta Life Sciences Limited Serum half-life extended pd-l1 binding polypeptides
WO2023150753A1 (en) 2022-02-07 2023-08-10 University Of Rochester Optimized sequences for enhanced trna expression or/and nonsense mutation suppression
WO2023224829A1 (en) * 2022-05-16 2023-11-23 Vector Surgical, Llc Intracellular treatment device and methods of use thereof
WO2024102187A1 (en) 2022-11-07 2024-05-16 Pinetree Therapeutics, Inc. Combination therapy comprising bispecific antibodies comprising an nrp1 binding domain
WO2024129459A1 (en) 2022-12-16 2024-06-20 University Of Rochester Repairmen! of barrier dysfunction in esophagus

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1099062A (en) * 1913-12-10 1914-06-02 Peter P Laposkey Electric scalp appliance.
US3773051A (en) * 1972-03-01 1973-11-20 Research Corp Method and apparatus for stimulation of body tissue
US4116238A (en) * 1976-08-18 1978-09-26 Midgard Electronics Company, Inc. High voltage constant current source for iontophoresis
US4528265A (en) * 1982-05-11 1985-07-09 Becker Robert O Processes and products involving cell modification
US4784757A (en) * 1987-07-13 1988-11-15 Nelson Edgar E Solid material separator
US5128257A (en) * 1987-08-31 1992-07-07 Baer Bradford W Electroporation apparatus and process
ATE131081T1 (de) * 1988-01-21 1995-12-15 Massachusetts Inst Technology Molekültransport durch gewebe mit der verwendung von elektroporation.
US5389069A (en) * 1988-01-21 1995-02-14 Massachusetts Institute Of Technology Method and apparatus for in vivo electroporation of remote cells and tissue
US4955378A (en) * 1988-05-02 1990-09-11 University Of South Florida Apparatus and methods for performing electrofusion at specific anatomical sites
WO1989010690A1 (en) * 1988-05-02 1989-11-16 University Of South Florida Method for electrofusing biological particles to tissues
CS275292B2 (en) * 1989-02-22 1992-02-19 Cvut Fakulta Elektrotechnick Private rehabilitation apparatus with ion transcutaneous acceleration
US5036850A (en) * 1989-08-25 1991-08-06 Staodyn, Inc. Biphasic pulse output stage for electronic stimulating device
US5097833A (en) * 1989-09-19 1992-03-24 Campos James M Transcutaneous electrical nerve and/or muscle stimulator
US5137817A (en) * 1990-10-05 1992-08-11 Amoco Corporation Apparatus and method for electroporation
US5507781A (en) * 1991-05-23 1996-04-16 Angeion Corporation Implantable defibrillator system with capacitor switching circuitry
US5411525A (en) * 1992-01-30 1995-05-02 Cardiac Pacemakers, Inc. Dual capacitor biphasic defibrillator waveform generator employing selective connection of capacitors for each phase
US5273525A (en) * 1992-08-13 1993-12-28 Btx Inc. Injection and electroporation apparatus for drug and gene delivery
US5688233A (en) * 1992-08-17 1997-11-18 Genetronics, Inc. Electronincorporation enhanced transdermal delivery of molecules
US5318514A (en) * 1992-08-17 1994-06-07 Btx, Inc. Applicator for the electroporation of drugs and genes into surface cells
US5468223A (en) * 1992-11-30 1995-11-21 C.N.R.S. Paris Electrochemotherapy
FR2703253B1 (fr) * 1993-03-30 1995-06-23 Centre Nat Rech Scient Applicateur d'impulsions electriques pour traitement de tissus biologiques.
US5993434A (en) * 1993-04-01 1999-11-30 Genetronics, Inc. Method of treatment using electroporation mediated delivery of drugs and genes
US5439440A (en) * 1993-04-01 1995-08-08 Genetronics, Inc. Electroporation system with voltage control feedback for clinical applications
US5702359A (en) * 1995-06-06 1997-12-30 Genetronics, Inc. Needle electrodes for mediated delivery of drugs and genes
US6041252A (en) * 1995-06-07 2000-03-21 Ichor Medical Systems Inc. Drug delivery system and method
US6261281B1 (en) * 1997-04-03 2001-07-17 Electrofect As Method for genetic immunization and introduction of molecules into skeletal muscle and immune cells
EA002087B1 (ru) * 1997-04-03 2001-12-24 Электрофект Ас Способ введения фармацевтических препаратов и нуклеиновых кислот в скелетную мышцу
US5873849A (en) * 1997-04-24 1999-02-23 Ichor Medical Systems, Inc. Electrodes and electrode arrays for generating electroporation inducing electrical fields
US6009347A (en) * 1998-01-27 1999-12-28 Genetronics, Inc. Electroporation apparatus with connective electrode template

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