RU94024562A - Antibody, polynucleotide, cell, method of producing an antibody - Google Patents

Antibody, polynucleotide, cell, method of producing an antibody

Info

Publication number
RU94024562A
RU94024562A RU94024562/13A RU94024562A RU94024562A RU 94024562 A RU94024562 A RU 94024562A RU 94024562/13 A RU94024562/13 A RU 94024562/13A RU 94024562 A RU94024562 A RU 94024562A RU 94024562 A RU94024562 A RU 94024562A
Authority
RU
Russia
Prior art keywords
hiv
antibody
antibodies
amino acid
acid sequence
Prior art date
Application number
RU94024562/13A
Other languages
Russian (ru)
Other versions
RU2134724C1 (en
Inventor
Охно Тсунея
Original Assignee
Ниссин Сокухин Кабусики Кайся
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US1992/007111 external-priority patent/WO1993004090A1/en
Application filed by Ниссин Сокухин Кабусики Кайся filed Critical Ниссин Сокухин Кабусики Кайся
Priority claimed from PCT/US1993/007967 external-priority patent/WO1994004574A1/en
Publication of RU94024562A publication Critical patent/RU94024562A/en
Application granted granted Critical
Publication of RU2134724C1 publication Critical patent/RU2134724C1/en

Links

Claims (1)

Настоящее изобретение представляет моноклональные антитела, которые специфически иммунореактивны с белком gpl20 ВИЧ-1 или его предшественником, белком gpl60, содержащем аминокислотную последовательность, представленную в ПОСЛ ИДN 1, G-P-G-R, и характеризуются способностью нейтрализовать in vitro инфицирование клеток Н9 живыми штаммами MN и IIIв ВИЧ-1, что определено с помощью исследований на обратную транскриптазу, р24, МТ-2 и образования синцития. Предпочтительнее в настоящее время антитела NM-01, выделенные из мышь/мышиной гибридом АТСС НВ 10726, кроме того, охарактеризованы по их способности опосредовать зависимый от комплемента виролиз частиц ВНЧ-1 и зависимую от антител клеточную цитотоксичность для клеток, инфицированных ВИЧ-1. В частности, раскрываются антитела, состоящие по существу из вариабельной области человеческого антитела, включающей последовательность аминокислот из по крайней мере одной области, определяющей комплементарность моноклонального антитела, продуцируемого гибридомной клеточной линией АТСС НВ 10726. Фармацевтические композиции этого изобретения, как предполагается, будут пригодны для лечения пассивной иммунизацией животных, особенно людей, восприимчивых или инфицированных ВИЧ-1.The present invention provides monoclonal antibodies that are specifically immunoreactive with HIV-1 gpl20 protein or its precursor, gpl60 protein, containing the amino acid sequence shown in SEQ ID No. 1, GPGR, and are capable of neutralizing in vitro infection of H9 cells with live MN and III strains in HIV -1, as determined by reverse transcriptase, p24, MT-2 and syncytium studies. Presently, NM-01 antibodies isolated from a mouse / mouse ATCC HB 10726 hybrid are preferred, moreover, characterized by their ability to mediate complement-dependent virolysis of VNCH-1 particles and antibody-dependent cellular cytotoxicity for HIV-1 infected cells. In particular, antibodies are disclosed that consist essentially of the variable region of a human antibody, comprising an amino acid sequence from at least one region that determines the complementarity of a monoclonal antibody produced by the hybridoma cell line ATCC HB 10726. The pharmaceutical compositions of this invention are expected to be suitable for treatment passive immunization of animals, especially people susceptible or infected with HIV-1.
RU94024562A 1992-08-24 1993-08-24 Humanized antibody and method of preparation thereof, polynucleotyl (versions), and hybridon cell line (versions) RU2134724C1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
PCT/US1992/007111 WO1993004090A1 (en) 1991-08-22 1992-08-24 Hiv immunotherapeutics
US92/07111 1992-08-24
US08/039457 1993-04-22
PCT/US1993/007967 WO1994004574A1 (en) 1991-08-22 1993-08-24 Hiv immunotherapeutics

Publications (2)

Publication Number Publication Date
RU94024562A true RU94024562A (en) 1996-03-27
RU2134724C1 RU2134724C1 (en) 1999-08-20

Family

ID=22236878

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94024562A RU2134724C1 (en) 1992-08-24 1993-08-24 Humanized antibody and method of preparation thereof, polynucleotyl (versions), and hybridon cell line (versions)

Country Status (1)

Country Link
RU (1) RU2134724C1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6580570B2 (en) * 2013-09-06 2019-09-25 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Methods for improving antibody stability

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