RU93004936A - DNA, STRAINS, METHODS FOR PRODUCING DNA, METHODS FOR PRODUCING POLYPEPTIDES - Google Patents

DNA, STRAINS, METHODS FOR PRODUCING DNA, METHODS FOR PRODUCING POLYPEPTIDES

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Publication number
RU93004936A
RU93004936A RU93004936/13A RU93004936A RU93004936A RU 93004936 A RU93004936 A RU 93004936A RU 93004936/13 A RU93004936/13 A RU 93004936/13A RU 93004936 A RU93004936 A RU 93004936A RU 93004936 A RU93004936 A RU 93004936A
Authority
RU
Russia
Prior art keywords
methods
dna
producing
dictyostelium
recombinant
Prior art date
Application number
RU93004936/13A
Other languages
Russian (ru)
Inventor
Жозеф Фазель Никола
Доминик Реймонд Кристоф
Original Assignee
РМФ Диктажен С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by РМФ Диктажен С.А. filed Critical РМФ Диктажен С.А.
Publication of RU93004936A publication Critical patent/RU93004936A/en

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Claims (1)

Изобретение относится к рекомбинантным молекулам ДНК, состоящим из области промотора, гомологичного Dictyostelium discoideum гетерологичной последовательности ДНК, копирующей пептид, гомологичный Dictyostelium discoideum, способной функционировать как последовательность лидерного пептида, расположенной перед промотором и в соответствующей с ним рамке считывания, области терминации, гомологичной Dictyostelium discoideum, последовательности гетерологичной ДНК, кодирующей заданный функциональный полипептид или его посредник, причем указанная последовательность ДНК расположена после промотора и указанная область терминации расположена за последовательностью ДНК. Изобретение также предусматривает создание рекомбинантных хозяйских клеток Dictyostelium, а также способы получения рекомбинантных молекул ДНК согласно изобретению и способы экспрессии рекомбинантных полипептидов, кодируемых указанными рекомбинантными молекулами ДНК в хозяйских клетках Dictyostelium. В частности, изобретение относится к экспрессии белка ОС Plasmodium falciparum.The invention relates to recombinant DNA molecules consisting of a promoter region homologous to Dictyostelium discoideum a heterologous DNA sequence copying a peptide homologous to Dictyostelium discoideum capable of functioning as a leader peptide sequence located in front of the promoter and in the corresponding reading frame, a termination region homologous to disiumcoic a sequence of heterologous DNA encoding a given functional polypeptide or its intermediary, wherein said DNA sequence located after the promoter and the specified termination region is located behind the DNA sequence. The invention also provides for the creation of recombinant Dictyostelium host cells, as well as methods for producing recombinant DNA molecules according to the invention and methods for expressing recombinant polypeptides encoded by said recombinant DNA molecules in Dictyostelium host cells. In particular, the invention relates to the expression of an OS protein of Plasmodium falciparum.
RU93004936/13A 1991-05-17 1992-05-18 DNA, STRAINS, METHODS FOR PRODUCING DNA, METHODS FOR PRODUCING POLYPEPTIDES RU93004936A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL9100869 1991-05-17

Publications (1)

Publication Number Publication Date
RU93004936A true RU93004936A (en) 1996-09-27

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