RU92014490A - METHOD FOR PRODUCING HIGH PURIFIED PHOSPHATASE FROM THIN INTESTINE OF SEAL - Google Patents

METHOD FOR PRODUCING HIGH PURIFIED PHOSPHATASE FROM THIN INTESTINE OF SEAL

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Publication number
RU92014490A
RU92014490A RU92014490/13A RU92014490A RU92014490A RU 92014490 A RU92014490 A RU 92014490A RU 92014490/13 A RU92014490/13 A RU 92014490/13A RU 92014490 A RU92014490 A RU 92014490A RU 92014490 A RU92014490 A RU 92014490A
Authority
RU
Russia
Prior art keywords
enzyme
seal
producing high
high purified
sorbent
Prior art date
Application number
RU92014490/13A
Other languages
Russian (ru)
Other versions
RU2036236C1 (en
Inventor
В.П. Варламов
А.И. Гамзазаде
Original Assignee
В.П. Варламов
А.И. Гамзазаде
Filing date
Publication date
Application filed by В.П. Варламов, А.И. Гамзазаде filed Critical В.П. Варламов
Priority to RU92014490A priority Critical patent/RU2036236C1/en
Priority claimed from RU92014490A external-priority patent/RU2036236C1/en
Application granted granted Critical
Publication of RU2036236C1 publication Critical patent/RU2036236C1/en
Publication of RU92014490A publication Critical patent/RU92014490A/en

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Claims (1)

Предлагаемое изобретение относится к биотехнологии и препаративной биохимии и может быть использовано в области иммуноферментного анализа. Цель: упрощение способа и удешевление его с одновременным повышением степени очистки фермента. Поставленная цель достигается использованием в качестве сорбента аминопропилированного кремнезема с диаметром пор 500-3000
Figure 00000001
с ковалентно присоединенным N-сульфосукцинатом хитозана в количестве 0,5-50 мг/мл сорбента. Согласно предлагаемого изобретения получена высокоочищенная щелочная фосфатаза с удельной активностью 4000-5000 ед./мг белка. Полученный продукт пригоден в качестве фермента маркера в высокочувствительном иммуноферментном анализе.The present invention relates to biotechnology and preparative biochemistry and can be used in the field of enzyme immunoassay. Purpose: to simplify the method and reduce its cost while increasing the degree of purification of the enzyme. This goal is achieved by using aminopropylated silica as a sorbent with a pore diameter of 500-3000
Figure 00000001
with covalently attached N-sulfosuccinate chitosan in an amount of 0.5-50 mg / ml sorbent. According to the invention, a highly purified alkaline phosphatase with a specific activity of 4000-5000 units / mg of protein is obtained. The resulting product is suitable as a marker enzyme in a highly sensitive enzyme-linked immunosorbent assay.
RU92014490A 1992-12-25 1992-12-25 Method of purification of alkaline phosphatase from seal intestine RU2036236C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU92014490A RU2036236C1 (en) 1992-12-25 1992-12-25 Method of purification of alkaline phosphatase from seal intestine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU92014490A RU2036236C1 (en) 1992-12-25 1992-12-25 Method of purification of alkaline phosphatase from seal intestine

Publications (2)

Publication Number Publication Date
RU2036236C1 RU2036236C1 (en) 1995-05-27
RU92014490A true RU92014490A (en) 1997-03-20

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU92014490A RU2036236C1 (en) 1992-12-25 1992-12-25 Method of purification of alkaline phosphatase from seal intestine

Country Status (1)

Country Link
RU (1) RU2036236C1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100222638B1 (en) * 1997-01-20 1999-10-01 배희동 Process for producing enzymes using the seeds
US11628381B2 (en) 2012-09-17 2023-04-18 W.R. Grace & Co. Conn. Chromatography media and devices

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