RU2013121503A - METHOD FOR PROTECTING CELLS FROM APOPTOSIS - Google Patents
METHOD FOR PROTECTING CELLS FROM APOPTOSIS Download PDFInfo
- Publication number
- RU2013121503A RU2013121503A RU2013121503/15A RU2013121503A RU2013121503A RU 2013121503 A RU2013121503 A RU 2013121503A RU 2013121503/15 A RU2013121503/15 A RU 2013121503/15A RU 2013121503 A RU2013121503 A RU 2013121503A RU 2013121503 A RU2013121503 A RU 2013121503A
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- apoptosis
- glutathione
- cells
- protecting cells
- concentration
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Способ оценки эффективности защиты клеток от апоптоза путем определения концентрации глутатиона, отличающийся тем, что при дополнительном добавлении в инкубационную среду лимфоцитов 1,4-дитиоэритритола и аскорбиновой кислоты в конечной концентрации 3.0 мМ и 0,1 мМ соответственно рост белково-связанного глутатиона на 18% и более и восстановленного глутатиона на 25% и более в культуре клеток свидетельствует об эффектиной защите клеток от апоптоза.A method for evaluating the effectiveness of protecting cells from apoptosis by determining the concentration of glutathione, characterized in that with the additional addition of 1,4-dithioerythritol and ascorbic acid to the incubation medium at a final concentration of 3.0 mM and 0.1 mM, respectively, the growth of protein-bound glutathione by 18% and more and reduced glutathione by 25% or more in cell culture indicates effective protection of cells from apoptosis.
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RU2013121503/15A RU2541774C2 (en) | 2013-05-07 | 2013-05-07 | Method for cell protection against apoptosis |
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RU2013121503/15A RU2541774C2 (en) | 2013-05-07 | 2013-05-07 | Method for cell protection against apoptosis |
Publications (2)
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RU2013121503A true RU2013121503A (en) | 2014-11-20 |
RU2541774C2 RU2541774C2 (en) | 2015-02-20 |
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RU2013121503/15A RU2541774C2 (en) | 2013-05-07 | 2013-05-07 | Method for cell protection against apoptosis |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2568886C1 (en) * | 2014-11-25 | 2015-11-20 | Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт кардиологии" | Method for assessing effectiveness of lymphocyte protection against apoptosis |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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US20070293458A1 (en) * | 2006-06-16 | 2007-12-20 | Ip-6 Research Inc. | Prevention of nuclear, solar, and other radiation-induced tissue damage |
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- 2013-05-07 RU RU2013121503/15A patent/RU2541774C2/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2568886C1 (en) * | 2014-11-25 | 2015-11-20 | Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт кардиологии" | Method for assessing effectiveness of lymphocyte protection against apoptosis |
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RU2541774C2 (en) | 2015-02-20 |
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MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20150508 |