RU2012130940A - Дифференцировка человеческих эмбриональных стволовых клеток - Google Patents

Дифференцировка человеческих эмбриональных стволовых клеток Download PDF

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RU2012130940A
RU2012130940A RU2012130940/10A RU2012130940A RU2012130940A RU 2012130940 A RU2012130940 A RU 2012130940A RU 2012130940/10 A RU2012130940/10 A RU 2012130940/10A RU 2012130940 A RU2012130940 A RU 2012130940A RU 2012130940 A RU2012130940 A RU 2012130940A
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tirfostina
line
cells expressing
markers characteristic
expressing markers
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Джанет ДЭВИС
Кристин ПАРМЕНТЕР
Кевин ДИТОЛВО
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Янссен Байотек, Инк.
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Abstract

1. Способ увеличения экспрессии NGN3 и NKX6.1 в популяции клеток, экспрессирующих маркеры, характерные для эндокринной линии поджелудочной железы, включающий следующие стадии:a) культивирования плюрипотентных стволовых клеток,b) дифференцировки плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии дефинитивной эндодермы,c) дифференцировки клеток, экспрессирующих маркеры, характерные для линии дефинитивной эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатической эндодермы, путем добавления в среду, используемую для дифференцировки линии дефинитивной эндодермы, соединений, выбираемых из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7, иd) дифференцировки клеток, экспрессирующих маркеры, характерные для линии панкреатической эндодермы, в клетки, экспресмирующие маркеры, характерные для эндокринной линии поджелудочной железы.2. Способ по п.1, где к среде, используемой для дифференцировки линии панкреатической эндодермы, добавляют соединения, выбираемые из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7.

Claims (2)

1. Способ увеличения экспрессии NGN3 и NKX6.1 в популяции клеток, экспрессирующих маркеры, характерные для эндокринной линии поджелудочной железы, включающий следующие стадии:
a) культивирования плюрипотентных стволовых клеток,
b) дифференцировки плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии дефинитивной эндодермы,
c) дифференцировки клеток, экспрессирующих маркеры, характерные для линии дефинитивной эндодермы, в клетки, экспрессирующие маркеры, характерные для линии панкреатической эндодермы, путем добавления в среду, используемую для дифференцировки линии дефинитивной эндодермы, соединений, выбираемых из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7, и
d) дифференцировки клеток, экспрессирующих маркеры, характерные для линии панкреатической эндодермы, в клетки, экспресмирующие маркеры, характерные для эндокринной линии поджелудочной железы.
2. Способ по п.1, где к среде, используемой для дифференцировки линии панкреатической эндодермы, добавляют соединения, выбираемые из группы, которая состоит из H-9, H-89, GF 109203X, HA-1004, PP2, PP1, LY 294002, Вортманнина, SB-203580, SB-202190, Тирфостина 25, Тирфостина, AG1478, Тирфостина 46, GW 5074, Кенпауллона, HNMPA, AG490, Y27632 и ML-7.
RU2012130940/10A 2009-12-23 2010-12-16 Дифференцировка человеческих эмбриональных стволовых клеток RU2586506C2 (ru)

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PCT/US2010/060770 WO2011079018A2 (en) 2009-12-23 2010-12-16 Differentiation of human embryonic stem cells

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