RU2006104627A - Измерение реакции амплификации полинуклеотидов - Google Patents

Измерение реакции амплификации полинуклеотидов Download PDF

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Publication number
RU2006104627A
RU2006104627A RU2006104627/13A RU2006104627A RU2006104627A RU 2006104627 A RU2006104627 A RU 2006104627A RU 2006104627/13 A RU2006104627/13 A RU 2006104627/13A RU 2006104627 A RU2006104627 A RU 2006104627A RU 2006104627 A RU2006104627 A RU 2006104627A
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Russia
Prior art keywords
molecule
amplification reaction
detection
polynucleotide
detecting
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RU2006104627/13A
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English (en)
Inventor
Дэниел Генри ДЕНШЕМ (GB)
Дэниел Генри ДЕНШЕМ
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Дэниел Генри ДЕНШЕМ (GB)
Дэниел Генри ДЕНШЕМ
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Priority claimed from GB0316555A external-priority patent/GB0316555D0/en
Priority claimed from GB0328425A external-priority patent/GB0328425D0/en
Application filed by Дэниел Генри ДЕНШЕМ (GB), Дэниел Генри ДЕНШЕМ filed Critical Дэниел Генри ДЕНШЕМ (GB)
Publication of RU2006104627A publication Critical patent/RU2006104627A/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/628—Detection means characterised by use of a special device being a surface plasmon resonance spectrometer

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (18)

1. Способ мониторинга амплификации множества различных полинуклеотидов-мишеней в единой реакционной камере, включающий этапы:
1) проведение реакции амплификации множества различных полинуклеотидов-мишеней,
2) в процессе реакции амплификации контакт различных амплифицированных продуктов с молекулой, которая связывается или взаимодействует с полинуклеотидом, молекула локализована в пространственно определенном положении или определена с помощью нелинейной или нефлуоресцентной методики, и
3) детекцию взаимодействия между амплифицированным продуктом и молекулой путем измерения изменений в примененном излучении.
2. Способ по п.1, где молекула иммобилизована на материале подложки.
3. Способ по п.1, где молекулой является фермент полимераза.
4. Способ по п.1, где молекулой является полинуклеотид, по меньшей мере часть которого комплементарна области амплифицированного продукта.
5. Способ по п.4, где молекула действует как праймер для реакции амплификации.
6. Способ по п.4, где молекула не действует как праймер для реакции амплификации.
7. Способ по любому предшествующему пункту, где детекцию на этапе (3) выполняют путем детекции затухающего поля.
8. Способ по любому из пп.1-6, где детекцию на этапе (3) выполняют путем применения поверхностных электромагнитных волн и мониторинга изменений в волнах.
9. Способ по любому из пп.1-6, где детекцию выполняют путем измерения изменений поверхностного плазменного резонанса.
10. Способ по п.9, где молекула содержит металлическую частицу.
11. Способ по любому из пп.1-6 где детекцию на этапе (2) выполняют путем детекции поверхности, усиленной рамановским рассеянием.
12. Способ по любому из пп.4-6, где детекция на этапе (2) достигается путем детекции интеркалированной метки, которая связывается с гибридом, образованным между амплифицированным продуктом и полинуклеотидом в процессе реакции амплификации.
13. Способ по п.12, где интеркалированной меткой является флуоресцент, когда связан с гибридом.
14. Способ по любому из пп.1-6, где детекция на этапе (3) достигается путем мониторинга изменений электрической проводимости и/или емкости.
15. Способ по любому из пп.1-6, где реакция амплификации происходит в герметичной проточной микроячейке.
16. Устройство для мониторинга реакции амплификации полинуклеотидов, содержащее материал подложки, имеющий множество иммобилизованных на нем молекул, где молекулы имеют способность связываться или взаимодействовать с полинуклеотидом и предназначены для детекции изменений в примененном излучении.
17. Устройство по п.16, дополнительно содержащее герметичную проточную микроячейку.
18. Устройство по п.16 или 17, дополнительно содержащее насос для поддержания потока жидкости над материалом подложки.
RU2006104627/13A 2003-07-15 2004-07-15 Измерение реакции амплификации полинуклеотидов RU2006104627A (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0316555A GB0316555D0 (en) 2003-07-15 2003-07-15 Method
GB0316555.2 2003-07-15
GB0328425.4 2003-12-08
GB0328425A GB0328425D0 (en) 2003-12-08 2003-12-08 Method

Publications (1)

Publication Number Publication Date
RU2006104627A true RU2006104627A (ru) 2006-09-10

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Country Status (11)

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US (1) US20070122808A1 (ru)
EP (1) EP1644524A1 (ru)
JP (1) JP2007529999A (ru)
KR (1) KR20060058681A (ru)
AU (1) AU2004257918B2 (ru)
BR (1) BRPI0412555A (ru)
CA (1) CA2532220A1 (ru)
IL (1) IL173114A0 (ru)
IS (1) IS8298A (ru)
RU (1) RU2006104627A (ru)
WO (1) WO2005007887A1 (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2446863C1 (ru) * 2010-09-10 2012-04-10 Сергей Михайлович Кузьмин Способ изготовления мембранного фильтра

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GB0413082D0 (en) * 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
JP2008543279A (ja) * 2005-06-09 2008-12-04 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 磁気検出を伴った核酸の増幅
KR20090067174A (ko) * 2006-09-14 2009-06-24 더 리전트 오브 더 유니버시티 오브 캘리포니아 뉴클레아제 활성 및 ｄｎａ 풋프린팅을 위한 나노플라즈몬 분자 계측자
EP1992702A1 (en) * 2007-05-14 2008-11-19 Koninklijke Philips Electronics N.V. Detection methods with increased accuracy and/or sensitivity
EP2077336A1 (en) * 2007-12-19 2009-07-08 Koninklijke Philips Electronics N.V. Device and method for parallel quantitative analysis of multiple nucleic acids
EP2138588A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Melting curve measurement during amplification
EP2141246A1 (en) * 2008-07-01 2010-01-06 Koninklijke Philips Electronics N.V. Separation-free methods of PCR detection using SERRS
JP2012509078A (ja) * 2008-11-21 2012-04-19 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 二本鎖核酸特異色素を用いる固体表面でのリアルタイムマルチプレックスｐｃｒ検出
DE102008059985B3 (de) * 2008-12-02 2010-04-01 Ip Bewertungs Ag Real-Time-PCR mittels Gigahertz- oder Terahertz-Spektrometrie
JP6393967B2 (ja) * 2013-09-05 2018-09-26 セイコーエプソン株式会社 ラマン分光装置、ラマン分光法、および電子機器

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DE10132785A1 (de) * 2001-07-06 2003-01-16 Clondiag Chip Tech Gmbh Verfahren zum Nachweis von in einer Polymerase-Kettenreaktion amplifizierten Nukleinsäuremolekülen
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2446863C1 (ru) * 2010-09-10 2012-04-10 Сергей Михайлович Кузьмин Способ изготовления мембранного фильтра

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IL173114A0 (en) 2006-06-11
KR20060058681A (ko) 2006-05-30
IS8298A (is) 2006-02-13
JP2007529999A (ja) 2007-11-01
AU2004257918A1 (en) 2005-01-27
BRPI0412555A (pt) 2006-09-19
US20070122808A1 (en) 2007-05-31
AU2004257918B2 (en) 2008-07-03
WO2005007887A1 (en) 2005-01-27
CA2532220A1 (en) 2005-01-27
EP1644524A1 (en) 2006-04-12

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