RU2005123794A - Способ определения концентрации исследуемого вещества в физиологической пробе - Google Patents

Способ определения концентрации исследуемого вещества в физиологической пробе Download PDF

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Publication number
RU2005123794A
RU2005123794A RU2005123794/28A RU2005123794A RU2005123794A RU 2005123794 A RU2005123794 A RU 2005123794A RU 2005123794/28 A RU2005123794/28 A RU 2005123794/28A RU 2005123794 A RU2005123794 A RU 2005123794A RU 2005123794 A RU2005123794 A RU 2005123794A
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Prior art keywords
concentration
test substance
time
current
sample
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RU2005123794/28A
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Inventor
Тимоти Дж. ОХАРА (US)
Тимоти Дж. ОХАРА
Махиар З. КЕРМАНИ (US)
Махиар З. КЕРМАНИ
Аластэр ХОДЖЕС (US)
Аластэр ХОДЖЕС
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Лайфскен, Инк. (Us)
Лайфскен, Инк.
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Publication of RU2005123794A publication Critical patent/RU2005123794A/ru

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Claims (3)

1. Способ определения концентрации исследуемого вещества в физиологической пробе, включающий в себя:
(a) введение упомянутой физиологической пробы в электрохимическую ячейку, содержащую:
(i) отстоящие друг от друга рабочий электрод и электрод сравнения; и
(ii) систему окислительно-восстановительных реактивов, содержащую фермент и медиатор;
(b) приложение к упомянутой электрохимической ячейке первого электрического потенциала, имеющего первую полярность, и измерение тока в ячейке как функции времени для получения первой переходной характеристики время-ток;
(c) приложение к упомянутой электрохимической ячейке второго электрического потенциала, имеющего вторую полярность, противоположную упомянутой первой полярности, и измерение тока в ячейке как функции времени для получения второй переходной характеристики время-ток;
(d) выведение предварительной концентрации исследуемого вещества на основании упомянутых первой и/или второй переходных характеристик время-ток; и
(е) умножение упомянутой предварительной концентрации исследуемого вещества за вычетом значения фона на коэффициент поправки для выведения скорректированной концентрации исследуемого вещества в упомянутой пробе;
посредством чего определяют скорректированную концентрацию упомянутого исследуемого вещества в упомянутой пробе.
2. Способ по п.1, в котором упомянутая физиологическая проба представляет собой цельную кровь или ее производное.
3. Способ по п.1, в котором упомянутое исследуемое вещество представляет собой глюкозу.
RU2005123794/28A 2000-02-02 2005-07-26 Способ определения концентрации исследуемого вещества в физиологической пробе RU2005123794A (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/497,304 2000-02-02
US09/497,304 US6475372B1 (en) 2000-02-02 2000-02-02 Electrochemical methods and devices for use in the determination of hematocrit corrected analyte concentrations

Publications (1)

Publication Number Publication Date
RU2005123794A true RU2005123794A (ru) 2007-02-10

Family

ID=23976305

Family Applications (2)

Application Number Title Priority Date Filing Date
RU2002116217/14A RU2262890C2 (ru) 2000-02-02 2001-01-25 Электрохимический способ и устройства, предназначенные для применения при определении концентраций исследуемых веществ с поправкой на гематокритное число
RU2005123794/28A RU2005123794A (ru) 2000-02-02 2005-07-26 Способ определения концентрации исследуемого вещества в физиологической пробе

Family Applications Before (1)

Application Number Title Priority Date Filing Date
RU2002116217/14A RU2262890C2 (ru) 2000-02-02 2001-01-25 Электрохимический способ и устройства, предназначенные для применения при определении концентраций исследуемых веществ с поправкой на гематокритное число

Country Status (21)

Country Link
US (3) US6475372B1 (ru)
EP (2) EP1645876A3 (ru)
JP (1) JP2003521708A (ru)
KR (1) KR20020077409A (ru)
CN (1) CN1193228C (ru)
AR (1) AR027345A1 (ru)
AT (1) ATE313794T1 (ru)
AU (1) AU783311B2 (ru)
CA (1) CA2398203A1 (ru)
CZ (1) CZ20021298A3 (ru)
DE (1) DE60116056T2 (ru)
DK (1) DK1252514T3 (ru)
ES (1) ES2254368T3 (ru)
HK (1) HK1049881B (ru)
IL (1) IL149662A (ru)
MX (1) MXPA02005797A (ru)
MY (1) MY127335A (ru)
PL (1) PL358492A1 (ru)
RU (2) RU2262890C2 (ru)
TW (1) TW520439B (ru)
WO (1) WO2001057510A2 (ru)

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