RU2004116344A - Способы детекции клеток с аномалиями в числе хромосом - Google Patents
Способы детекции клеток с аномалиями в числе хромосом Download PDFInfo
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- RU2004116344A RU2004116344A RU2004116344/13A RU2004116344A RU2004116344A RU 2004116344 A RU2004116344 A RU 2004116344A RU 2004116344/13 A RU2004116344/13 A RU 2004116344/13A RU 2004116344 A RU2004116344 A RU 2004116344A RU 2004116344 A RU2004116344 A RU 2004116344A
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6875—Nucleoproteins
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (15)
1. Способ детекции аномалий в числе хромосом, включающий в себя обработку митотических клеток митотическим маркером и количественным красителем ДНК и определение количества ДНК в указанной клетке с применением указанного количественного красителя ДНК.
2. Способ по п.1, где указанное определение количества ДНК включает в себя применение проточной цитометрии.
3. Способ по п.1, дополнительно включающий в себя характеристику указанной клетки как содержащий аномалию в числе хромосом при условии, что указанную клетку определяют указанным количественным анализом как содержащую большее количество ДНК или меньшее количество ДНК, чем количество ДНК в клетке с нормальной плоидностью.
4. Способ идентификации клетки с аномалией в числе хромосом в популяции клеток, включающий в себя обработку популяции клеток митотическим маркером и количественным красителем ДНК и идентификации клетки в указанной популяции как обладающей аномалией в числе хромосом, при условии, что указанную клетку идентифицируют как митотическую указанным митотическим маркером и дополнительно при условии, что указанную клетку указанным количественным красителем ДНК определяют как содержащую большее количество ДНК или меньшее количество ДНК, чем количество ДНК в клетке с нормальной плоидностью.
5. Способ по п.4, где для идентификации клетки как митотической и для определения того, что указанная клетка содержит большее количество ДНК или меньшее количество ДНК, чем количество ДНК в клетке с нормальной плоидностью, применяют клеточную цитометрию.
6. Способ по п.1 или 4, где митотический маркер представляет собой средство, которое детектирует фосфорилирование гистона, происходящее во время митоза.
7. Способ по п. 1 или 4, где митотический маркер представляет собой моноклональное антитело 6G3.
8. Способ по п.1 или 4, где количественный краситель ДНК выбирают из интеркалирующего красителя, связывающего малую борозду средства, цианинового красителя и йодида пропидия.
9. Способ по п.1 или 4, где количественный краситель ДНК представляет собой йодид пропидия.
10. Способ выделения клеток с аномалией в числе хромосом из популяции клеток, включающий в себя обработку популяции клеток митотическим маркером и количественным красителем ДНК, идентификацию клетки в указанной популяции как обладающую аномалией в числе хромосом и выделение указанной клетки при условии, что указанную клетку идентифицируют как митотическую указанным митотическим маркером, и дополнительно при условии, что указанную клетку указанным количественным красителем ДНК определяют как содержащую большее количество ДНК или меньшее количество ДНК, чем количество ДНК в клетке с нормальной плоидностью.
11. Способ по п. 10, где указанная клеточная популяция содержит, по меньшей мере, две субпопуляции клеток, где каждая обладает различной аномалией в числе хромосом, и где указанный способ дополнительно включает в себя отделение указанных субпопуляций клеток друг от друга и от указанной клеточной популяции.
12. Анализ для идентификации средства, индуцирующего аномалии в числе хромосом в клетке, включающий обработку клетки тестируемым средством, обработку указанной клетки или ее потомства митотическим маркером и количественным красителем ДНК и определения количества ДНК в указанной клетке с применением указанного количественного красителя ДНК, при условии, что указанная клетка или ее потомство являются митотическими в течение воздействия митотическим маркером и количественным красителем ДНК.
13. Анализ по п. 12, где указанная клетка представляет собой мононуклеарную клетку периферической крови, лимфоцит, эпителиальную клетку и клетку соединительной ткани.
14. Набор для детекции аномалий в числе хромосом, включающий в себя митотический маркер и количественный краситель ДНК.
15. Композиция, включающая митотический маркер и количественный краситель ДНК.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33728201P | 2001-11-30 | 2001-11-30 | |
US60/337,282 | 2001-11-30 |
Publications (1)
Publication Number | Publication Date |
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RU2004116344A true RU2004116344A (ru) | 2005-03-27 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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RU2004116344/13A RU2004116344A (ru) | 2001-11-30 | 2002-11-20 | Способы детекции клеток с аномалиями в числе хромосом |
Country Status (12)
Country | Link |
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US (1) | US20030104461A1 (ru) |
EP (1) | EP1316619A1 (ru) |
JP (1) | JP2003210198A (ru) |
KR (1) | KR20040062979A (ru) |
CN (1) | CN1606628A (ru) |
AU (1) | AU2002347468A1 (ru) |
CA (1) | CA2413177A1 (ru) |
HR (1) | HRP20040488A2 (ru) |
IL (1) | IL161856A0 (ru) |
NO (1) | NO20042180L (ru) |
RU (1) | RU2004116344A (ru) |
WO (1) | WO2003046218A1 (ru) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2004029221A2 (en) * | 2002-09-27 | 2004-04-08 | The General Hospital Corporation | Microfluidic device for cell separation and uses thereof |
GB0319376D0 (en) * | 2003-08-18 | 2003-09-17 | Chroma Therapeutics Ltd | Histone modification detection |
US7625712B2 (en) * | 2004-05-21 | 2009-12-01 | Beckman Coulter, Inc. | Method for a fully automated monoclonal antibody-based extended differential |
EP2594631A1 (en) * | 2005-04-05 | 2013-05-22 | Cellpoint Diagnostics | Devices and method for detecting circulating tumor cells and other particles |
US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
US8101368B2 (en) * | 2006-02-13 | 2012-01-24 | Dvs Sciences Inc. | Quantitation of cellular DNA and cell numbers using element labeling |
FR2902440B1 (fr) * | 2006-06-19 | 2008-08-29 | Univ Paris Curie | Methode de detection et de denombrement des cellules en cytodierese |
FR2907220B1 (fr) * | 2006-10-11 | 2009-01-16 | Reactifs Ral Sa | Procede de coloration de cellules |
CA2707673A1 (en) * | 2007-12-10 | 2009-06-01 | Ben Gurion University Of The Negev Research And Development Authority | Nucleic acid detection assay |
EP2112509A1 (en) * | 2008-03-25 | 2009-10-28 | 4Sc Ag | Centrosome-assay |
CN102250825A (zh) * | 2011-06-22 | 2011-11-23 | 首都师范大学 | 一种采用流式细胞仪分选正在***细胞的方法 |
US11410751B2 (en) * | 2016-05-27 | 2022-08-09 | Life Technologies Corporation | Methods and systems for graphical user interfaces for biological data |
CA3096537A1 (en) * | 2018-04-09 | 2019-10-17 | Inguran, Llc | Improved sperm nuclei and methods of their manufacture and use |
CN108866146A (zh) * | 2018-07-04 | 2018-11-23 | 中国医学科学院放射医学研究所 | 一种细胞周期检测试剂盒 |
US20210389225A1 (en) * | 2018-12-01 | 2021-12-16 | Mingdao Innovation (Beijing) Medical-Tech Co., Ltd. | A flow cytometric detection method for lymphocyte in immune cells |
CN111307696A (zh) * | 2020-03-19 | 2020-06-19 | 浙江星博生物科技股份有限公司 | 用于检测***dna碎片率的方法和试剂盒 |
US11952593B2 (en) * | 2020-10-06 | 2024-04-09 | Pharmacogenetics Limited | Detection and treatment of daughter number variations in cancer cells |
Family Cites Families (7)
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US4780406A (en) * | 1983-10-18 | 1988-10-25 | The Regents Of The University Of California | Flow cytometric measurement of total DNA and incorporated halodeoxyuridine |
US6203977B1 (en) * | 1988-11-15 | 2001-03-20 | Yale University | Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization |
US5807999A (en) * | 1993-02-01 | 1998-09-15 | Mt. Sinai School Of Medicine Of The City University Of New York | Monoclonal antibody that distinguishes between phosphorylated and nonphosphorylated histone H1 and uses therefor |
US6362317B1 (en) * | 1999-07-12 | 2002-03-26 | The United States Of America As Represented By The Department Of Health And Human Services | Anti-γ-H2A antibody, fusion proteins thereof and method and kit for determining DNA double-stranded breaks |
AU2001275072A1 (en) * | 2000-05-31 | 2001-12-11 | The Scripps Research Institute | Molecular marker for mitosis |
US6555329B2 (en) * | 2000-06-09 | 2003-04-29 | Boehringer Ingelheim International Gmbh | Method for identifying compounds altering higher-order chromatin-dependent chromosome stability |
AU2002327433A1 (en) * | 2001-08-10 | 2003-02-24 | Cornell Research Foundation, Inc. | Telomerase immortalized human neutral stem cells and phenotypically-restricted progenitor cells |
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2002
- 2002-11-20 RU RU2004116344/13A patent/RU2004116344A/ru not_active Application Discontinuation
- 2002-11-20 CN CNA028239873A patent/CN1606628A/zh active Pending
- 2002-11-20 AU AU2002347468A patent/AU2002347468A1/en not_active Abandoned
- 2002-11-20 IL IL16185602A patent/IL161856A0/xx unknown
- 2002-11-20 KR KR10-2004-7008252A patent/KR20040062979A/ko not_active Application Discontinuation
- 2002-11-20 WO PCT/IB2002/004856 patent/WO2003046218A1/en not_active Application Discontinuation
- 2002-11-22 EP EP02258072A patent/EP1316619A1/en not_active Withdrawn
- 2002-11-27 US US10/306,944 patent/US20030104461A1/en not_active Abandoned
- 2002-11-28 CA CA002413177A patent/CA2413177A1/en not_active Abandoned
- 2002-11-29 JP JP2002347133A patent/JP2003210198A/ja active Pending
-
2004
- 2004-05-26 NO NO20042180A patent/NO20042180L/no unknown
- 2004-05-31 HR HR20040488A patent/HRP20040488A2/hr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
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CN1606628A (zh) | 2005-04-13 |
JP2003210198A (ja) | 2003-07-29 |
US20030104461A1 (en) | 2003-06-05 |
IL161856A0 (en) | 2005-11-20 |
KR20040062979A (ko) | 2004-07-09 |
WO2003046218A1 (en) | 2003-06-05 |
EP1316619A1 (en) | 2003-06-04 |
HRP20040488A2 (en) | 2005-06-30 |
NO20042180L (no) | 2004-08-26 |
AU2002347468A1 (en) | 2003-06-10 |
CA2413177A1 (en) | 2003-05-30 |
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