RU2003137558A - Получение биологически доступной фолиевой кислоты - Google Patents
Получение биологически доступной фолиевой кислоты Download PDFInfo
- Publication number
- RU2003137558A RU2003137558A RU2003137558/13A RU2003137558A RU2003137558A RU 2003137558 A RU2003137558 A RU 2003137558A RU 2003137558/13 A RU2003137558/13 A RU 2003137558/13A RU 2003137558 A RU2003137558 A RU 2003137558A RU 2003137558 A RU2003137558 A RU 2003137558A
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- Russia
- Prior art keywords
- microorganism according
- microorganism
- enzyme
- activity
- increased activity
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- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 title claims 3
- 235000019152 folic acid Nutrition 0.000 title claims 3
- 239000011724 folic acid Substances 0.000 title claims 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 title 1
- 229960000304 folic acid Drugs 0.000 title 1
- 244000005700 microbiome Species 0.000 claims 15
- 108090000790 Enzymes Proteins 0.000 claims 7
- 102000004190 Enzymes Human genes 0.000 claims 7
- 230000000694 effects Effects 0.000 claims 7
- 108090000623 proteins and genes Proteins 0.000 claims 5
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims 4
- FJUSJWBIWLXZEN-GJZGRUSLSA-N (4S)-4-amino-5-[(4S)-4-[[4-[(2-amino-4-oxo-3H-pteridin-6-yl)methylamino]benzoyl]amino]-4-carboxybutanoyl]oxy-5-oxopentanoic acid Chemical compound N[C@@H](CCC(O)=O)C(=O)OC(CC[C@@H](C(=O)O)NC(=O)C1=CC=C(NCC2=CN=C3N=C(N)NC(=O)C3=N2)C=C1)=O FJUSJWBIWLXZEN-GJZGRUSLSA-N 0.000 claims 3
- 235000013305 food Nutrition 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 2
- 241000124008 Mammalia Species 0.000 claims 2
- 235000014655 lactic acid Nutrition 0.000 claims 2
- 239000004310 lactic acid Substances 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 102000036509 GTP Cyclohydrolase Human genes 0.000 claims 1
- 108010023555 GTP Cyclohydrolase Proteins 0.000 claims 1
- 102100021023 Gamma-glutamyl hydrolase Human genes 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 235000013365 dairy product Nutrition 0.000 claims 1
- 229940014144 folate Drugs 0.000 claims 1
- 150000002224 folic acids Chemical class 0.000 claims 1
- 108010062699 gamma-Glutamyl Hydrolase Proteins 0.000 claims 1
- 210000001035 gastrointestinal tract Anatomy 0.000 claims 1
- 101150102771 gch gene Proteins 0.000 claims 1
- 235000013617 genetically modified food Nutrition 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/45—Transferases (2)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/50—Hydrolases (3) acting on carbon-nitrogen bonds, other than peptide bonds (3.5), e.g. asparaginase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1235—Diphosphotransferases (2.7.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/182—Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C2220/00—Biochemical treatment
- A23C2220/20—Treatment with microorganisms
- A23C2220/202—Genetic engineering of microorganisms used in dairy technology
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Nutrition Science (AREA)
- Polymers & Plastics (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Diabetes (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Obesity (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (14)
1. Генетически измененный пищевой микроорганизм, в котором модификация приводит к увеличению количества продуцированного моноглутамилфолата по сравнению с соответствующим количеством, продуцированным немодифицированным микроорганизмом.
2. Микроорганизм по п.1, в котором, по меньшей мере, один фермент, участвующий в синтезе моноглутамилфолата, обладает повышенной активностью.
3. Микроорганизм по п.2, в котором указанный фермент содержит GTP циклогидролазу, кодируемую gch геном.
4. Микроорганизм по п.1, в котором гамма-глутамилгидролаза обладает повышенной активностью.
5. Микроорганизм по п.2 или 3, в котором повышенная активность фермента является следствием наличия множества копий соответствующих генов.
6. Микроорганизм по п.2 или 3, в котором повышенная активность фермента является следствием присутствия промотора, усиливающего экспрессию соответствующих генов.
7. Микроорганизмы по п.1, в котором гены, кодирующие ферменты с указанной активностью, имеют животное происхождение, и, главным образом, происходят из млекопитающих.
8. Микроорганизм по п.1, в котором гены, кодирующие ферментаты с указанной активностью, имеют растительное происхождение.
9. Микроорганизм по п.1, в котором гены, кодирующие ферменты с указанной активностью, происходят из пищевых микроорганизмов, в особенности из молочнокислых бактерий или дрожжей.
10. Микроорганизм по п.1, представляющий собой молочнокислую бактерию.
11. Способ получения моноглутамилфолата, включающий культивирование микроорганизма по любому из пп.1-10 и выделение продуцированных фолатов.
12. Способ доставки биологически доступного фолата в желудочно-кишечный тракт млекопитающих, заключающийся в применении эффективного количества микроорганизма по любому из пп.1-10.
13. Пищевой продукт, содержащий микроорганизм по любому из пп.1-10.
14. Пищевой продукт по п.13, представляющий собой молочный продукт.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01202013.7 | 2001-05-28 | ||
| EP01202013A EP1262541A1 (en) | 2001-05-28 | 2001-05-28 | Production of bioavailable folic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2003137558A true RU2003137558A (ru) | 2005-05-20 |
| RU2342426C2 RU2342426C2 (ru) | 2008-12-27 |
Family
ID=8180380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2003137558/13A RU2342426C2 (ru) | 2001-05-28 | 2002-05-28 | Получение биологически доступной фолиевой кислоты |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20040214261A1 (ru) |
| EP (2) | EP1262541A1 (ru) |
| JP (1) | JP2004527262A (ru) |
| CN (1) | CN1513056A (ru) |
| BR (1) | BR0209727A (ru) |
| CZ (1) | CZ20033565A3 (ru) |
| HU (1) | HUP0400435A3 (ru) |
| PL (1) | PL368444A1 (ru) |
| RU (1) | RU2342426C2 (ru) |
| SK (1) | SK16332003A3 (ru) |
| WO (1) | WO2002097063A1 (ru) |
| ZA (1) | ZA200309155B (ru) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101939441B (zh) | 2007-12-06 | 2014-05-07 | 普拉克生化公司 | 增加乳杆菌发酵瓜类汁液中的叶酸盐产生水平 |
| CN101776665B (zh) * | 2009-01-13 | 2013-09-11 | 上海医药工业研究院 | 一种乳酸链球菌素的hplc检测方法 |
| CN105624182A (zh) * | 2015-12-15 | 2016-06-01 | 重庆大学 | 生产四氢叶酸的重组质粒、基因工程菌株构建及应用 |
| CN110300534A (zh) * | 2017-02-17 | 2019-10-01 | 皇家飞利浦有限公司 | 温度控制设备、食物烹饪设备和用于控制烹饪食物的加热单元的方法 |
| WO2021035421A1 (en) * | 2019-08-23 | 2021-03-04 | Chifeng Pharmaceutical Co., Ltd. | Folate producing strain and the preparation and application thereof |
| WO2023180103A1 (en) * | 2022-03-21 | 2023-09-28 | Immundiagnostik Ag | Kit of parts and microbiological method for assessment of the folate status in serum and red blood cells |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2135423T3 (es) * | 1992-03-03 | 1999-11-01 | Meiji Seika Kaisha | Gamma-poliglutamato-hidrolasa y procedimiento de produccion de esta. |
| DE19929363A1 (de) * | 1999-06-25 | 2000-12-28 | Basf Lynx Bioscience Ag | Gene aus Corynebacterium glutamicum für die Folsäurebiosynthese und ihr Einsatz zur mikrobiellen Herstellung von Folsäure |
-
2001
- 2001-05-28 EP EP01202013A patent/EP1262541A1/en not_active Withdrawn
-
2002
- 2002-05-28 WO PCT/NL2002/000340 patent/WO2002097063A1/en active Application Filing
- 2002-05-28 EP EP02733616A patent/EP1392816A1/en not_active Withdrawn
- 2002-05-28 CN CNA028109740A patent/CN1513056A/zh active Pending
- 2002-05-28 JP JP2003500232A patent/JP2004527262A/ja active Pending
- 2002-05-28 RU RU2003137558/13A patent/RU2342426C2/ru not_active IP Right Cessation
- 2002-05-28 PL PL02368444A patent/PL368444A1/xx not_active Application Discontinuation
- 2002-05-28 US US10/478,994 patent/US20040214261A1/en not_active Abandoned
- 2002-05-28 BR BR0209727-3A patent/BR0209727A/pt not_active IP Right Cessation
- 2002-05-28 CZ CZ20033565A patent/CZ20033565A3/cs unknown
- 2002-05-28 HU HU0400435A patent/HUP0400435A3/hu unknown
- 2002-05-28 SK SK1633-2003A patent/SK16332003A3/sk unknown
-
2003
- 2003-11-25 ZA ZA200309155A patent/ZA200309155B/en unknown
-
2008
- 2008-02-19 US US12/033,655 patent/US20080248553A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| PL368444A1 (en) | 2005-03-21 |
| ZA200309155B (en) | 2004-11-25 |
| US20080248553A1 (en) | 2008-10-09 |
| BR0209727A (pt) | 2004-09-14 |
| JP2004527262A (ja) | 2004-09-09 |
| EP1262541A1 (en) | 2002-12-04 |
| HUP0400435A3 (en) | 2005-02-28 |
| CN1513056A (zh) | 2004-07-14 |
| WO2002097063A1 (en) | 2002-12-05 |
| RU2342426C2 (ru) | 2008-12-27 |
| CZ20033565A3 (cs) | 2004-04-14 |
| HUP0400435A2 (hu) | 2004-11-29 |
| EP1392816A1 (en) | 2004-03-03 |
| SK16332003A3 (sk) | 2004-07-07 |
| US20040214261A1 (en) | 2004-10-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20110529 |