RO72669B1 - Enterobacterial vaccine for veterinary use and process for preparing the same - Google Patents

Abstract

Vaccin eterobacteral de uz veterinar caracterizat prin aceea ca este constituit din parti egale pe unitatea de volum de antigen corpuscular si antigen solubil din genurile Escherichia coli, Klebsiella, Salomella, Pseudomonas, Proteus, Providencia, Citrobacter, si Aerobacter, în urmatoarele procente de participare a genurilor: 30.....35% Escherichia coli, 10....16% Klebsiella, 15.....20% Salmonella, 15....20% Pseudomonas, 15....20% Proteus si eventual 0....6% Providencia, Citrobacter si Aerobacter singure sau asociate, numarul final de germeni continut în vaccin fiind de circa 21 miliarde pe ml vaccin, determinat pe scara Brown. Procedeul de preparare a vaccinului conform revendicarii caracterizat prin aceea ca, tulpinile se izoleaza si se tripleaza din organe ale animalelor bolnave cum ar fi creier, ficat, splina, rinichi, duoden, ileon, maduva osoasa prin metode bacterologice cunoscute, tulpinile izolate se însamînteaza separat pe bulion glucozat, se incubeaza 18.....24 h cu aerare la37 degree C si pH = 8,2.....8,5 se lasa pH-ul culturilor tinde sa se stabileze la pH=7.....7,2 dupa care culturile se incubeaza la termostat timp de 60 h, se inactiveaza termic la 65....70 degree C timp de 6....8 h adaugîndu-se fiecarei culturi 0,8% clorura de ca lciu sicc, cu circa 30....60 min înaintea opririi inactivarii, apoi culturile sunt supuse inactivarii prin iradiere cu raze ultraviolete prin sticla obisnuita timp de 8....10 h, iar în final suspensiile tratate cu mertiolat de sodiu se amesteca în proportiile indicate.Veterinary etibacterial vaccine characterized in that it consists of equal parts per unit volume of corpuscular antigen and soluble antigen of the genera Escherichia coli, Klebsiella, Salomella, Pseudomonas, Proteus, Providencia, Citrobacter, and Aerobacter, in the following percentages of genes: 30 ..... 35% Escherichia coli, 10 ... 16% Klebsiella, 15 ..... 20% Salmonella, 15 ... 20% Pseudomonas, 15 ... 20% Proteus possibly 0 ... 6% Providencia, Citrobacter and Aerobacter alone or associated, the final count of germs contained in the vaccine being about 21 billion per ml vaccine, determined on the Brown scale. The process for preparing the vaccine according to the claim characterized in that the strains are isolated and triple from organs of diseased animals such as the brain, liver, spleen, kidney, duodenum, ileum, bone marrow by known bacteriological methods, isolated strains are separately harvested on the glucose broth, incubate 18 ..... 24 h with aeration at 37 ° C and pH = 8.2 ..... 8.5 let the pH of the cultures tend to set at pH = 7 ... .7.2 after which the cultures are incubated on the thermostat for 60 hours, heat inactivate at 65 ... 70 ° C for 6 ... 8 hours, adding to each culture 0.8% of the chloride lice sicc with about 30-60 minutes before the inactivation stops, then the cultures are subjected to inactivation by ultraviolet irradiation with the usual glass for 8-10 hours and finally the suspensions treated with sodium mertiolate are mixed in the indicated proportions.