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Application filed by Cantacuzino InstfiledCriticalCantacuzino Inst
Priority to RO138173ApriorityCriticalpatent/RO103684B1/en
Publication of RO103684A2publicationCriticalpatent/RO103684A2/en
Publication of RO103684B1publicationCriticalpatent/RO103684B1/en
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents
(AREA)
Peptides Or Proteins
(AREA)
Abstract
The process comprises defibrination with CaCl2 of plasma very high in IgA, and very low in IgG, for treatment with 25 g of (NH4)2SO4 per 100 ml at 4 deg.C. The centrifuged ppte is washed two hours with soln. of (NH4)2SO4 (25%, 25g per 100 ml of water), and buffered with Na3PO4/H3PO4, 0.03 M, pH = 5, containing 0.3 M of NaCl and 0.02 per cent of NaN3. Chromatography, and elution, with buffer K2HPO4, 0.02 M, pH = 8, and further chromatography, is followed by elution with 0.1 M NaCl in K2HPO4, 0.02 M, pH = 8.
RO138173A1989-02-101989-02-10Production method of immuno-globulin a1(iga1)
RO103684B1
(en)