PT1159448E - Determinacao de formas biologicamente activas de enzimas proteoliticas - Google Patents

Determinacao de formas biologicamente activas de enzimas proteoliticas

Info

Publication number
PT1159448E
PT1159448E PT00914107T PT00914107T PT1159448E PT 1159448 E PT1159448 E PT 1159448E PT 00914107 T PT00914107 T PT 00914107T PT 00914107 T PT00914107 T PT 00914107T PT 1159448 E PT1159448 E PT 1159448E
Authority
PT
Portugal
Prior art keywords
substrate
proteolytic enzyme
molecules
group
activated
Prior art date
Application number
PT00914107T
Other languages
English (en)
Inventor
Hendrik Coenraad Hemker
Robert Jan Wagenvoord
Manoj Ramjee
Original Assignee
Synapse Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synapse Bv filed Critical Synapse Bv
Publication of PT1159448E publication Critical patent/PT1159448E/pt

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10Anilides
    • C12Q2337/12Para-Nitroanilides p-NA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/20Coumarin derivatives
    • C12Q2337/227-Amino-4-methylcoumarin, i.e. AMC, MCA
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/76Assays involving albumins other than in routine use for blocking surfaces or for anchoring haptens during immunisation
    • G01N2333/77Ovalbumin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81Protease inhibitors
    • G01N2333/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/811Serine protease (E.C. 3.4.21) inhibitors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PT00914107T 1999-03-04 2000-03-03 Determinacao de formas biologicamente activas de enzimas proteoliticas PT1159448E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99200627 1999-03-04
US15724099P 1999-10-01 1999-10-01

Publications (1)

Publication Number Publication Date
PT1159448E true PT1159448E (pt) 2004-08-31

Family

ID=44350544

Family Applications (1)

Application Number Title Priority Date Filing Date
PT00914107T PT1159448E (pt) 1999-03-04 2000-03-03 Determinacao de formas biologicamente activas de enzimas proteoliticas

Country Status (9)

Country Link
EP (1) EP1159448B1 (pt)
JP (1) JP4721519B2 (pt)
AT (1) ATE264401T1 (pt)
AU (1) AU3553900A (pt)
DE (1) DE60009879T2 (pt)
DK (1) DK1159448T3 (pt)
ES (1) ES2219324T3 (pt)
PT (1) PT1159448E (pt)
WO (1) WO2000052199A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040241782A1 (en) 2001-01-26 2004-12-02 Eloisa Lopez-Calle Methods and means for detecting enzymatic cleavage and linkage reactions
JP4718833B2 (ja) * 2002-05-01 2011-07-06 シナプス ベスローテン フェンノートシャップ 複合生物媒体における、一時的なタンパク質分解活性の濃度を決定するための診断試験
DE102005008066A1 (de) 2005-02-22 2006-08-24 Haemosys Gmbh Verfahren zur Bestimmung der Gesamtgerinnungsaktivität einer Blut- oder Plasmaprobe
DE102006035899A1 (de) * 2006-07-31 2008-02-07 Dade Behring Marburg Gmbh Polysacharid-Peptid-Konjugate zur Verwendung als Thrombinsubstrate
CA2668356A1 (en) * 2006-11-02 2008-05-08 Thrombinoscope B.V. Method for measuring the concentration of transient proteolytic activity in composite biological media containing cells
EP2206063A1 (en) 2007-10-16 2010-07-14 Koninklijke Philips Electronics N.V. Estimation of diagnostic markers
RU2518247C2 (ru) * 2011-07-26 2014-06-10 Общество с ограниченной ответственностью "ГемаКор Лабс" (ООО "ГемаКор Лабс") Способ определения пространственно-временного распределения активности протеолитического фермента в гетерогенной системе, устройство для реализации указанного способа и способ диагностики нарушений системы гемостаза по изменению пространственно-временного распределения активности протеолитического фермента в гетерогенной системе
EP3363912B1 (en) 2017-02-17 2020-10-28 Synapse B.V. Self-calibrated assay for determining proteolytic enzyme generation, dedicated means and uses thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2363854C3 (de) * 1973-12-21 1980-01-03 Behringwerke Ag, 3550 Marburg Substrat zur Proteinasebestimmung sowie dessen Herstellung
US4897444A (en) * 1985-05-31 1990-01-30 The Research Foundation Of The State University Of New York Immobilized fluorogenic substrates for enzymes; and processes for their preparation
US5073487A (en) * 1989-01-30 1991-12-17 Athens Research And Technology, Inc. Rapid assay for functional human α1 -proteinase inhibitor
AU4634896A (en) * 1995-01-10 1996-07-31 Hendrik Coenraad Hemker Methods of determining endogenous thrombin potential (etp) and thrombin substrates for use in said methods

Also Published As

Publication number Publication date
DE60009879D1 (de) 2004-05-19
DK1159448T3 (da) 2004-08-09
DE60009879T2 (de) 2005-03-31
EP1159448B1 (en) 2004-04-14
JP4721519B2 (ja) 2011-07-13
EP1159448A1 (en) 2001-12-05
JP2002537820A (ja) 2002-11-12
AU3553900A (en) 2000-09-21
WO2000052199A1 (en) 2000-09-08
ATE264401T1 (de) 2004-04-15
ES2219324T3 (es) 2004-12-01

Similar Documents

Publication Publication Date Title
Goldstein et al. Use of water-insoluble enzyme derivatives in biochemical analysis and separation
EP2082057B1 (en) Detection of proteases
DE60009575D1 (de) Nachweis von bakterien und bakteriophagen mit immobilisierten enzymsubstraten
WO2001043764A3 (en) Methods for treating pervasive development disorders
ATE226642T1 (de) Modifizierte proenzyme als substrate für proteolische enzyme
PT1159448E (pt) Determinacao de formas biologicamente activas de enzimas proteoliticas
Ilaria et al. Pineapple stem bromelain immobilized on different supports: catalytic properties in model wine
ATE383413T1 (de) Verfahren und formulierungen zur erleichterung der auflösung eines feststoffs in flüssigkeit
SE8801701D0 (sv) Production technology based on enzymic method
WO2008081094A3 (fr) Methode de mesure fonctionnelle de l'activite de la thrombomoduline plasmatique
ATE269161T1 (de) Kontaktierungsvorrichtung
DK1624072T3 (da) Kit til bestemmelse af aktiviteten af faktor VII-aktiverende protease i proteinopløsninger
Blackburn The protease liberated from Bacteroides amylophilus strain H 18 by mechanical disintegration
EP1293779A3 (en) Biosensor surface
NO993297L (no) Fast dyrkningsmedium for mikroorganismer, fremgangsmÕte for fremstilling herav og anvendelse herav
Tang et al. Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor
BR9814334A (pt) Dispositivo de cultura para detecção e enumeração de microrganismos, e, processo para dividir uma amostra aquosa lìquida em microvolumes separados
Kok et al. Immobilization of acetylcholinesterase and choline oxidase in/on pHEMA membrane for biosensor construction
WO2002012543A3 (de) Zweifarbiges fluorimetrisches proteaseassay
EP1295948A3 (en) Method of measurement of protease and thin membranes used for said method
JP2002537820A5 (pt)
North et al. Purification and characterization of two acid proteinases from Dictyostelium discoideum
ATE407216T1 (de) Testsystem zur bestimmung von in-vivo aktiven proteasen der hämostase in biologischen flüssigkeiten
Jasim et al. A comparison of immobilised protease activities
ATE106447T1 (de) Verfahren zur gewinnung der protease von endothia parasitica.