PL440371A1 - Voltammetric immunosensor, method of pathogen detection using a voltammetric sensor, method of manufacturing a voltammetric sensor, its use for detecting pathogens, especially SARS-CoV-2 virus, and redox tracer - Google Patents

Voltammetric immunosensor, method of pathogen detection using a voltammetric sensor, method of manufacturing a voltammetric sensor, its use for detecting pathogens, especially SARS-CoV-2 virus, and redox tracer

Info

Publication number
PL440371A1
PL440371A1 PL440371A PL44037122A PL440371A1 PL 440371 A1 PL440371 A1 PL 440371A1 PL 440371 A PL440371 A PL 440371A PL 44037122 A PL44037122 A PL 44037122A PL 440371 A1 PL440371 A1 PL 440371A1
Authority
PL
Poland
Prior art keywords
voltammetric
immunosensor
redox
sensor
cov
Prior art date
Application number
PL440371A
Other languages
Polish (pl)
Inventor
Agata Kowalczyk
Anna Maria NOWICKA
Ireneusz Piotr Grudziński
Original Assignee
Uniwersytet Warszawski
Warszawski Uniwersytet Medyczny
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Warszawski, Warszawski Uniwersytet Medyczny filed Critical Uniwersytet Warszawski
Priority to PL440371A priority Critical patent/PL440371A1/en
Priority to PCT/IB2023/051292 priority patent/WO2023152717A1/en
Publication of PL440371A1 publication Critical patent/PL440371A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F17/00—Metallocenes
    • C07F17/02—Metallocenes of metals of Groups 8, 9 or 10 of the Periodic System
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Przedmiotem zgłoszenia jest immunoczujnik woltamperometryczny do wykrywania patogenów, który zawiera elektrodę modyfikowaną pierwszym przeciwciałem swoistym dla wykrywanego patogenu, które wiąże wykrywany patogen do powierzchni elektrody oraz drugie przeciwciało dla wykrywanego patogenu znakowane znacznikiem redoks, które wiąże się z wykrywanym patogenem związanym z powierzchnią elektrody, charakteryzujący się tym, że drugie przeciwciało jest swoiste dla wykrywanego patogenu. Zgłoszenie dotyczy także sposobu wykrywania patogenu z wykorzystaniem immunoczujnika woltamperometrycznego, sposobu wytwarzania immunoczujnika woltamperometrycznego, jego zastosowania w detekcji patogenów wirusowych, zwłaszcza wirusa SARS-CoV-2, a także znacznika redoks stosowanego w konstrukcji immunoczujnika woltamperometrycznego.The subject of the application is a voltammetric immunosensor for the detection of pathogens, which includes an electrode modified with a first antibody specific for the detected pathogen, which binds the detected pathogen to the electrode surface, and a second antibody for the detected pathogen labeled with a redox tag, which binds to the detected pathogen bound to the electrode surface, characterized by in that the second antibody is specific for the detected pathogen. The application also concerns a method of detecting a pathogen using a voltammetric immunosensor, a method of producing a voltammetric immunosensor, its use in the detection of viral pathogens, especially the SARS-CoV-2 virus, as well as a redox marker used in the construction of a voltammetric immunosensor.

PL440371A 2022-02-11 2022-02-11 Voltammetric immunosensor, method of pathogen detection using a voltammetric sensor, method of manufacturing a voltammetric sensor, its use for detecting pathogens, especially SARS-CoV-2 virus, and redox tracer PL440371A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL440371A PL440371A1 (en) 2022-02-11 2022-02-11 Voltammetric immunosensor, method of pathogen detection using a voltammetric sensor, method of manufacturing a voltammetric sensor, its use for detecting pathogens, especially SARS-CoV-2 virus, and redox tracer
PCT/IB2023/051292 WO2023152717A1 (en) 2022-02-11 2023-02-13 Voltammetric immunosensor, method of detecting pathogens using voltammetric sensor, method of producing voltammetric sensor, its use for detecting pathogens, especially sars-cov-2 virus, and redox probe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL440371A PL440371A1 (en) 2022-02-11 2022-02-11 Voltammetric immunosensor, method of pathogen detection using a voltammetric sensor, method of manufacturing a voltammetric sensor, its use for detecting pathogens, especially SARS-CoV-2 virus, and redox tracer

Publications (1)

Publication Number Publication Date
PL440371A1 true PL440371A1 (en) 2023-08-14

Family

ID=87563913

Family Applications (1)

Application Number Title Priority Date Filing Date
PL440371A PL440371A1 (en) 2022-02-11 2022-02-11 Voltammetric immunosensor, method of pathogen detection using a voltammetric sensor, method of manufacturing a voltammetric sensor, its use for detecting pathogens, especially SARS-CoV-2 virus, and redox tracer

Country Status (2)

Country Link
PL (1) PL440371A1 (en)
WO (1) WO2023152717A1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8504521D0 (en) * 1985-02-21 1985-03-27 Genetics Int Inc Electrochemical assay
US20210396703A1 (en) * 2020-06-17 2021-12-23 Northeastern University Rapid Electrochemical Point-of-Care COVID-19 Detection in Human Saliva

Also Published As

Publication number Publication date
WO2023152717A1 (en) 2023-08-17

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