PH12016501421B1 - PROCESS FOR EFFICIENT PURIFICATION OF NEUTRAL HUMAN MILK OLIGOSACCHARIDES (HMOs) FROM MICROBIAL FERMENTATION - Google Patents
PROCESS FOR EFFICIENT PURIFICATION OF NEUTRAL HUMAN MILK OLIGOSACCHARIDES (HMOs) FROM MICROBIAL FERMENTATIONInfo
- Publication number
- PH12016501421B1 PH12016501421B1 PH12016501421A PH12016501421A PH12016501421B1 PH 12016501421 B1 PH12016501421 B1 PH 12016501421B1 PH 12016501421 A PH12016501421 A PH 12016501421A PH 12016501421 A PH12016501421 A PH 12016501421A PH 12016501421 B1 PH12016501421 B1 PH 12016501421B1
- Authority
- PH
- Philippines
- Prior art keywords
- hmos
- neutral
- purification
- human milk
- microbial fermentation
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000007935 neutral effect Effects 0.000 title abstract 4
- 238000000746 purification Methods 0.000 title abstract 4
- 230000000813 microbial effect Effects 0.000 title abstract 3
- 238000000855 fermentation Methods 0.000 title abstract 2
- 230000004151 fermentation Effects 0.000 title abstract 2
- 210000004251 human milk Anatomy 0.000 title abstract 2
- 235000020256 human milk Nutrition 0.000 title abstract 2
- 229920001542 oligosaccharide Polymers 0.000 title abstract 2
- 150000002482 oligosaccharides Chemical class 0.000 title abstract 2
- 235000013305 food Nutrition 0.000 abstract 3
- 238000013375 chromatographic separation Methods 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 239000002178 crystalline material Substances 0.000 abstract 1
- 238000000909 electrodialysis Methods 0.000 abstract 1
- 238000012262 fermentative production Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000001728 nano-filtration Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/40—Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Pediatric Medicine (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Husbandry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fodder In General (AREA)
- Saccharide Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Dairy Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present application discloses a simple process for the purification of neutral human milk oligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of a cationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and/or electrodialysis step, which allows efficient purification of large quantities of neutral HMOs at high purity. Contrary to the purification currently used in fermentative production of neutral HMOs, the presented process allows the provision of HMOs without the need of a chromatographic separation. The so purified HMOs may be obtained in solid form by spray drying, as crystalline material or as sterile filtered concentrate. The provided HMOs are free of proteins and recombinant material originating from the used recombinant microbial strains and thus very well-suited for use in food, medical food and feed (e.g. pet food) applications.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14151737.5A EP2896628B1 (en) | 2014-01-20 | 2014-01-20 | Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation |
PCT/EP2014/079212 WO2015106943A1 (en) | 2014-01-20 | 2014-12-23 | PROCESS FOR EFFICIENT PURIFICATION OF NEUTRAL HUMAN MILK OLIGOSACCHARIDES (HMOs) FROM MICROBIAL FERMENTATION |
Publications (2)
Publication Number | Publication Date |
---|---|
PH12016501421B1 true PH12016501421B1 (en) | 2016-08-22 |
PH12016501421A1 PH12016501421A1 (en) | 2016-08-22 |
Family
ID=49949589
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PH12016501421A PH12016501421A1 (en) | 2014-01-20 | 2016-07-19 | PROCESS FOR EFFICIENT PURIFICATION OF NEUTRAL HUMAN MILK OLIGOSACCHARIDES (HMOs) FROM MICROBIAL FERMENTATION |
PH12020500199A PH12020500199A1 (en) | 2014-01-20 | 2020-01-27 | Process for efficient purification of neutral human milk oligosaccharides (hmos) from microbial fermentation |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PH12020500199A PH12020500199A1 (en) | 2014-01-20 | 2020-01-27 | Process for efficient purification of neutral human milk oligosaccharides (hmos) from microbial fermentation |
Country Status (18)
Country | Link |
---|---|
US (4) | US10377787B2 (en) |
EP (5) | EP2896628B1 (en) |
JP (2) | JP6901857B2 (en) |
KR (1) | KR102137027B1 (en) |
CN (1) | CN106132977B (en) |
AT (2) | AT17418U1 (en) |
AU (1) | AU2014377374B2 (en) |
BR (1) | BR112016016705A8 (en) |
CZ (2) | CZ35288U1 (en) |
DE (2) | DE202014011399U1 (en) |
DK (6) | DK2896628T3 (en) |
ES (4) | ES2701163T3 (en) |
FI (2) | FI3686211T3 (en) |
MX (2) | MX369375B (en) |
PH (2) | PH12016501421A1 (en) |
PL (4) | PL2896628T3 (en) |
RU (1) | RU2682445C2 (en) |
WO (1) | WO2015106943A1 (en) |
Families Citing this family (53)
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EP2896628B1 (en) | 2014-01-20 | 2018-09-19 | Jennewein Biotechnologie GmbH | Process for efficient purification of neutral human milk oligosaccharides (HMOs) from microbial fermentation |
US10500221B2 (en) | 2014-12-05 | 2019-12-10 | Glycom A/S | Crystalline difucosyllactose |
EP3390652A4 (en) | 2015-12-18 | 2019-06-12 | Glycom A/S | Fermentative production of oligosaccharides |
DE202017007249U1 (en) * | 2016-03-07 | 2020-04-23 | Glycom A/S | Separation of oligosaccharides from the fermentation broth |
WO2017182965A1 (en) * | 2016-04-19 | 2017-10-26 | Glycom A/S | Separation of oligosaccharides from fermentation broth |
US11142541B2 (en) | 2017-06-30 | 2021-10-12 | Glycom A/S | Purification of oligosaccharides |
CN110914284A (en) | 2017-07-12 | 2020-03-24 | 格礼卡姆股份公司 | Amorphous mixture comprising neutral mono-or oligosaccharides and acidic non-carbohydrate component |
EP3450443A1 (en) | 2017-08-29 | 2019-03-06 | Jennewein Biotechnologie GmbH | Process for purifying sialylated oligosaccharides |
EP3688005A1 (en) * | 2017-09-29 | 2020-08-05 | FrieslandCampina Nederland B.V. | Process for the purification of a neutral human milk oligosaccharide (hmo) from microbial fermentation |
EP3486326A1 (en) * | 2017-11-21 | 2019-05-22 | Jennewein Biotechnologie GmbH | Method for the purification of n-acetylneuraminic acid from a fermentation broth |
EP3494805A1 (en) | 2017-12-08 | 2019-06-12 | Jennewein Biotechnologie GmbH | Spray-dried tetrasaccharides |
EP3494804A1 (en) | 2017-12-08 | 2019-06-12 | Jennewein Biotechnologie GmbH | Spray-dried 3-fucosyllactose |
EP3524067A1 (en) * | 2018-02-08 | 2019-08-14 | Jennewein Biotechnologie GmbH | Spray-dried mixture of human milk oligosaccharides |
EP3494806A1 (en) | 2017-12-08 | 2019-06-12 | Jennewein Biotechnologie GmbH | Spray-dried lacto-n-fucopentaose |
JP2021505171A (en) * | 2017-12-08 | 2021-02-18 | イェンネワイン バイオテクノロジー ゲーエムベーハーJennewein Biotechnologie GmbH | Spray-dried sialyll lactose |
EP3494807A1 (en) | 2017-12-11 | 2019-06-12 | Jennewein Biotechnologie GmbH | Spray-dried sialyllactose |
EP3546060A1 (en) * | 2018-03-27 | 2019-10-02 | DuPont Nutrition Biosciences ApS | Process for spray drying fucosyllactose solutions and related product compositions |
JP7492458B2 (en) | 2018-05-07 | 2024-05-29 | クリスチャン・ハンセン・ハーエムオー・ゲーエムベーハー | A simple method for purifying lacto-N-neotetraose (LNnT) from carbohydrates obtained by microbial fermentation |
WO2019226431A1 (en) * | 2018-05-23 | 2019-11-28 | Dsm Ip Assets B.V. | Production of oligosaccharides |
FI3801037T3 (en) * | 2018-06-01 | 2023-01-13 | A simple method for the purification of a sialyllactose | |
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US20220081732A1 (en) * | 2019-01-21 | 2022-03-17 | Dupont Nutrition Biosciences Aps | Process for making l-fucose |
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