NZ727053B2 - Ornithodoros moubata complement inhibitor for use in the treatment of complement-mediated diseases in patients with c5 polymorphism - Google Patents
Ornithodoros moubata complement inhibitor for use in the treatment of complement-mediated diseases in patients with c5 polymorphism Download PDFInfo
- Publication number
- NZ727053B2 NZ727053B2 NZ727053A NZ72705315A NZ727053B2 NZ 727053 B2 NZ727053 B2 NZ 727053B2 NZ 727053 A NZ727053 A NZ 727053A NZ 72705315 A NZ72705315 A NZ 72705315A NZ 727053 B2 NZ727053 B2 NZ 727053B2
- Authority
- NZ
- New Zealand
- Prior art keywords
- complement
- protein
- amino acids
- seq
- acid sequence
- Prior art date
Links
- 230000000295 complement effect Effects 0.000 title claims abstract description 109
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 84
- 230000001404 mediated effect Effects 0.000 title claims abstract description 50
- 201000010099 disease Diseases 0.000 title claims abstract description 47
- 238000011282 treatment Methods 0.000 title description 33
- 241000238890 Ornithodoros moubata Species 0.000 title description 4
- 239000004074 complement inhibitor Substances 0.000 title description 3
- 229940124073 Complement inhibitor Drugs 0.000 title description 2
- 230000024203 complement activation Effects 0.000 claims abstract description 241
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 145
- 102000004856 Lectins Human genes 0.000 claims abstract description 60
- 108090001090 Lectins Proteins 0.000 claims abstract description 60
- 239000002523 lectin Substances 0.000 claims abstract description 60
- 102100031506 Complement C5 Human genes 0.000 claims abstract description 19
- 108010028773 Complement C5 Proteins 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 134
- 102000004169 proteins and genes Human genes 0.000 claims description 122
- 150000001413 amino acids Chemical class 0.000 claims description 62
- 229960002224 eculizumab Drugs 0.000 claims description 59
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 44
- 239000012634 fragment Substances 0.000 claims description 39
- 208000035475 disorder Diseases 0.000 claims description 36
- 239000003814 drug Substances 0.000 claims description 34
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 32
- 230000007423 decrease Effects 0.000 claims description 23
- 108010034753 Complement Membrane Attack Complex Proteins 0.000 claims description 17
- 238000003776 cleavage reaction Methods 0.000 claims description 16
- 230000007017 scission Effects 0.000 claims description 16
- 201000003045 paroxysmal nocturnal hemoglobinuria Diseases 0.000 claims description 14
- 206010043561 Thrombocytopenic purpura Diseases 0.000 claims description 12
- 208000009329 Graft vs Host Disease Diseases 0.000 claims description 11
- 208000024908 graft versus host disease Diseases 0.000 claims description 11
- 230000004044 response Effects 0.000 claims description 11
- 102000016574 Complement C3-C5 Convertases Human genes 0.000 claims description 9
- 108010067641 Complement C3-C5 Convertases Proteins 0.000 claims description 9
- 230000002949 hemolytic effect Effects 0.000 claims description 9
- 208000011580 syndromic disease Diseases 0.000 claims description 8
- 208000033808 peripheral neuropathy Diseases 0.000 claims description 7
- 230000001732 thrombotic effect Effects 0.000 claims description 7
- 238000012252 genetic analysis Methods 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 6
- 208000023504 respiratory system disease Diseases 0.000 claims description 6
- 102200094440 rs56040400 Human genes 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- 208000006673 asthma Diseases 0.000 claims description 5
- 230000006378 damage Effects 0.000 claims description 5
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 206010039073 rheumatoid arthritis Diseases 0.000 claims description 5
- 238000002054 transplantation Methods 0.000 claims description 5
- 206010064930 age-related macular degeneration Diseases 0.000 claims description 4
- 208000002780 macular degeneration Diseases 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 201000000596 systemic lupus erythematosus Diseases 0.000 claims description 4
- 208000027418 Wounds and injury Diseases 0.000 claims description 3
- 208000014674 injury Diseases 0.000 claims description 3
- 201000006417 multiple sclerosis Diseases 0.000 claims description 3
- 206010028417 myasthenia gravis Diseases 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 206010001052 Acute respiratory distress syndrome Diseases 0.000 claims description 2
- 208000024827 Alzheimer disease Diseases 0.000 claims description 2
- 208000032116 Autoimmune Experimental Encephalomyelitis Diseases 0.000 claims description 2
- 208000011231 Crohn disease Diseases 0.000 claims description 2
- 206010018364 Glomerulonephritis Diseases 0.000 claims description 2
- 206010019860 Hereditary angioedema Diseases 0.000 claims description 2
- 206010053159 Organ failure Diseases 0.000 claims description 2
- 206010033645 Pancreatitis Diseases 0.000 claims description 2
- 206010033647 Pancreatitis acute Diseases 0.000 claims description 2
- 208000018737 Parkinson disease Diseases 0.000 claims description 2
- 201000004681 Psoriasis Diseases 0.000 claims description 2
- 206010063837 Reperfusion injury Diseases 0.000 claims description 2
- 208000013616 Respiratory Distress Syndrome Diseases 0.000 claims description 2
- 206010040070 Septic Shock Diseases 0.000 claims description 2
- 208000006011 Stroke Diseases 0.000 claims description 2
- 208000030886 Traumatic Brain injury Diseases 0.000 claims description 2
- 201000003229 acute pancreatitis Diseases 0.000 claims description 2
- 208000011341 adult acute respiratory distress syndrome Diseases 0.000 claims description 2
- 201000000028 adult respiratory distress syndrome Diseases 0.000 claims description 2
- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 235000014633 carbohydrates Nutrition 0.000 claims description 2
- 238000001631 haemodialysis Methods 0.000 claims description 2
- 208000007475 hemolytic anemia Diseases 0.000 claims description 2
- 206010022000 influenza Diseases 0.000 claims description 2
- 208000028867 ischemia Diseases 0.000 claims description 2
- 208000010125 myocardial infarction Diseases 0.000 claims description 2
- 230000036303 septic shock Effects 0.000 claims description 2
- 230000009529 traumatic brain injury Effects 0.000 claims description 2
- 230000002792 vascular Effects 0.000 claims description 2
- 108010078546 Complement C5a Proteins 0.000 claims 5
- 208000037157 Azotemia Diseases 0.000 claims 1
- 102200094439 rs373359894 Human genes 0.000 claims 1
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract description 39
- 238000000034 method Methods 0.000 abstract description 29
- 230000000694 effects Effects 0.000 abstract description 20
- 150000002066 eicosanoids Chemical class 0.000 abstract description 10
- 235000018102 proteins Nutrition 0.000 description 94
- 210000002966 serum Anatomy 0.000 description 86
- 235000001014 amino acid Nutrition 0.000 description 35
- 238000011068 loading method Methods 0.000 description 34
- 238000012423 maintenance Methods 0.000 description 33
- 229940079593 drug Drugs 0.000 description 29
- 230000027455 binding Effects 0.000 description 27
- 241000282414 Homo sapiens Species 0.000 description 21
- 230000002354 daily effect Effects 0.000 description 20
- 230000037361 pathway Effects 0.000 description 13
- 230000002829 reductive effect Effects 0.000 description 13
- 230000004913 activation Effects 0.000 description 12
- 238000003556 assay Methods 0.000 description 11
- 230000005764 inhibitory process Effects 0.000 description 11
- 230000000903 blocking effect Effects 0.000 description 9
- 238000010254 subcutaneous injection Methods 0.000 description 9
- 239000007929 subcutaneous injection Substances 0.000 description 9
- 208000037765 diseases and disorders Diseases 0.000 description 8
- 108010076504 Protein Sorting Signals Proteins 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 7
- 108090000765 processed proteins & peptides Proteins 0.000 description 7
- 239000003112 inhibitor Substances 0.000 description 6
- 238000012421 spiking Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 230000004154 complement system Effects 0.000 description 5
- 239000003937 drug carrier Substances 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 238000006467 substitution reaction Methods 0.000 description 5
- 101100113588 Homo sapiens CS gene Proteins 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 241000238876 Acari Species 0.000 description 3
- 241000238421 Arthropoda Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 208000035895 Guillain-Barré syndrome Diseases 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 238000000099 in vitro assay Methods 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 201000001119 neuropathy Diseases 0.000 description 3
- 230000007823 neuropathy Effects 0.000 description 3
- 229950003203 pexelizumab Drugs 0.000 description 3
- 201000009732 pulmonary eosinophilia Diseases 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- -1 12S amino acids Chemical class 0.000 description 2
- 208000032671 Allergic granulomatous angiitis Diseases 0.000 description 2
- 206010001889 Alveolitis Diseases 0.000 description 2
- 208000035939 Alveolitis allergic Diseases 0.000 description 2
- 108010089414 Anaphylatoxins Proteins 0.000 description 2
- 206010057645 Chronic Inflammatory Demyelinating Polyradiculoneuropathy Diseases 0.000 description 2
- 208000030939 Chronic inflammatory demyelinating polyneuropathy Diseases 0.000 description 2
- 208000006344 Churg-Strauss Syndrome Diseases 0.000 description 2
- 241000258924 Ctenocephalides felis Species 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 206010014950 Eosinophilia Diseases 0.000 description 2
- 208000018428 Eosinophilic granulomatosis with polyangiitis Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010071602 Genetic polymorphism Diseases 0.000 description 2
- 208000007465 Giant cell arteritis Diseases 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 2
- RPTUSVTUFVMDQK-UHFFFAOYSA-N Hidralazin Chemical compound C1=CC=C2C(NN)=NN=CC2=C1 RPTUSVTUFVMDQK-UHFFFAOYSA-N 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 102000019298 Lipocalin Human genes 0.000 description 2
- 108050006654 Lipocalin Proteins 0.000 description 2
- 206010049459 Lymphangioleiomyomatosis Diseases 0.000 description 2
- 206010049567 Miller Fisher syndrome Diseases 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 208000037273 Pathologic Processes Diseases 0.000 description 2
- 241001674048 Phthiraptera Species 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 206010037660 Pyrexia Diseases 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 241000219061 Rheum Species 0.000 description 2
- 206010052779 Transplant rejections Diseases 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 208000027093 acute inflammatory demyelinating polyradiculoneuropathy Diseases 0.000 description 2
- 230000002730 additional effect Effects 0.000 description 2
- 206010003246 arthritis Diseases 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000003210 demyelinating effect Effects 0.000 description 2
- 201000001981 dermatomyositis Diseases 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 201000001155 extrinsic allergic alveolitis Diseases 0.000 description 2
- 230000037433 frameshift Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 208000022098 hypersensitivity pneumonitis Diseases 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 231100000516 lung damage Toxicity 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 210000000440 neutrophil Anatomy 0.000 description 2
- 230000009054 pathological process Effects 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000002685 pulmonary effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000004043 responsiveness Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 206010043207 temporal arteritis Diseases 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 238000011269 treatment regimen Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- WUBBRNOQWQTFEX-UHFFFAOYSA-N 4-aminosalicylic acid Chemical compound NC1=CC=C(C(O)=O)C(O)=C1 WUBBRNOQWQTFEX-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 239000005541 ACE inhibitor Substances 0.000 description 1
- 241000256111 Aedes <genus> Species 0.000 description 1
- 241000256118 Aedes aegypti Species 0.000 description 1
- 241000238682 Amblyomma americanum Species 0.000 description 1
- 241001480735 Amblyomma cajennense Species 0.000 description 1
- 241001480736 Amblyomma hebraeum Species 0.000 description 1
- 206010002556 Ankylosing Spondylitis Diseases 0.000 description 1
- 241000256186 Anopheles <genus> Species 0.000 description 1
- 241000256182 Anopheles gambiae Species 0.000 description 1
- 241001480752 Argas persicus Species 0.000 description 1
- 241001480754 Argas reflexus Species 0.000 description 1
- 108010000241 Arthropod Proteins Proteins 0.000 description 1
- 208000033116 Asbestos intoxication Diseases 0.000 description 1
- 206010003487 Aspergilloma Diseases 0.000 description 1
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 206010004485 Berylliosis Diseases 0.000 description 1
- 208000013883 Blast injury Diseases 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 239000005996 Blood meal Substances 0.000 description 1
- 206010006474 Bronchopulmonary aspergillosis allergic Diseases 0.000 description 1
- 208000007596 Byssinosis Diseases 0.000 description 1
- 208000020119 Caplan syndrome Diseases 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 229930186147 Cephalosporin Natural products 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- RKWGIWYCVPQPMF-UHFFFAOYSA-N Chloropropamide Chemical compound CCCNC(=O)NS(=O)(=O)C1=CC=C(Cl)C=C1 RKWGIWYCVPQPMF-UHFFFAOYSA-N 0.000 description 1
- 208000023355 Chronic beryllium disease Diseases 0.000 description 1
- 241000284156 Clerodendrum quadriloculare Species 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 208000025962 Crush injury Diseases 0.000 description 1
- 241000256054 Culex <genus> Species 0.000 description 1
- 241000256057 Culex quinquefasciatus Species 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 102100025621 Cytochrome b-245 heavy chain Human genes 0.000 description 1
- 244000115658 Dahlia pinnata Species 0.000 description 1
- 208000016192 Demyelinating disease Diseases 0.000 description 1
- 206010012305 Demyelination Diseases 0.000 description 1
- 241001480819 Dermacentor andersoni Species 0.000 description 1
- 241000227772 Dermacentor marginatus Species 0.000 description 1
- 241000577477 Dermacentor reticulatus Species 0.000 description 1
- 208000032131 Diabetic Neuropathies Diseases 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 208000027445 Farmer Lung Diseases 0.000 description 1
- 244000182067 Fraxinus ornus Species 0.000 description 1
- 235000002917 Fraxinus ornus Nutrition 0.000 description 1
- 208000012671 Gastrointestinal haemorrhages Diseases 0.000 description 1
- 241000257324 Glossina <genus> Species 0.000 description 1
- 208000024869 Goodpasture syndrome Diseases 0.000 description 1
- 206010072579 Granulomatosis with polyangiitis Diseases 0.000 description 1
- HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 1
- 241000233007 Haemaphysalis inermis Species 0.000 description 1
- VZJFGSRCJCXDSG-UHFFFAOYSA-N Hexamethonium Chemical compound C[N+](C)(C)CCCCCC[N+](C)(C)C VZJFGSRCJCXDSG-UHFFFAOYSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000545744 Hirudinea Species 0.000 description 1
- 241000750137 Hyalomma anatolicum anatolicum Species 0.000 description 1
- 201000009794 Idiopathic Pulmonary Fibrosis Diseases 0.000 description 1
- 102000009786 Immunoglobulin Constant Regions Human genes 0.000 description 1
- 108010009817 Immunoglobulin Constant Regions Proteins 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000029523 Interstitial Lung disease Diseases 0.000 description 1
- 241001480840 Ixodes hexagonus Species 0.000 description 1
- 241001480847 Ixodes persulcatus Species 0.000 description 1
- 241001480843 Ixodes ricinus Species 0.000 description 1
- 241000238703 Ixodes scapularis Species 0.000 description 1
- 201000005099 Langerhans cell histiocytosis Diseases 0.000 description 1
- 208000019693 Lung disease Diseases 0.000 description 1
- 206010025102 Lung infiltration Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010027406 Mesothelioma Diseases 0.000 description 1
- 229930192392 Mitomycin Natural products 0.000 description 1
- 208000026072 Motor neurone disease Diseases 0.000 description 1
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 241000092202 Ornithodoros erraticus Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- CXOFVDLJLONNDW-UHFFFAOYSA-N Phenytoin Chemical compound N1C(=O)NC(=O)C1(C=1C=CC=CC=1)C1=CC=CC=C1 CXOFVDLJLONNDW-UHFFFAOYSA-N 0.000 description 1
- 241000255129 Phlebotominae Species 0.000 description 1
- 241000242594 Platyhelminthes Species 0.000 description 1
- 206010035669 Pneumonia aspiration Diseases 0.000 description 1
- 206010035720 Pneumonia lipoid Diseases 0.000 description 1
- 229920000954 Polyglycolide Polymers 0.000 description 1
- 206010037549 Purpura Diseases 0.000 description 1
- 241001672981 Purpura Species 0.000 description 1
- 208000019155 Radiation injury Diseases 0.000 description 1
- 206010037764 Radiation myelopathy Diseases 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 208000035415 Reinfection Diseases 0.000 description 1
- 241001480837 Rhipicephalus annulatus Species 0.000 description 1
- 241001481704 Rhipicephalus appendiculatus Species 0.000 description 1
- 241000949016 Rhipicephalus bursa Species 0.000 description 1
- 241000864246 Rhipicephalus decoloratus Species 0.000 description 1
- 241000238680 Rhipicephalus microplus Species 0.000 description 1
- 240000000528 Ricinus communis Species 0.000 description 1
- 201000010001 Silicosis Diseases 0.000 description 1
- 241000256103 Simuliidae Species 0.000 description 1
- 241000258242 Siphonaptera Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 201000009594 Systemic Scleroderma Diseases 0.000 description 1
- 206010042953 Systemic sclerosis Diseases 0.000 description 1
- 241000255628 Tabanidae Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- IUJDSEJGGMCXSG-UHFFFAOYSA-N Thiopental Chemical compound CCCC(C)C1(CC)C(=O)NC(=S)NC1=O IUJDSEJGGMCXSG-UHFFFAOYSA-N 0.000 description 1
- 101100357018 Trypanosoma brucei brucei RNR2 gene Proteins 0.000 description 1
- 206010060875 Uraemic pruritus Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229960001138 acetylsalicylic acid Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000013572 airborne allergen Substances 0.000 description 1
- 208000006778 allergic bronchopulmonary aspergillosis Diseases 0.000 description 1
- 229960004909 aminosalicylic acid Drugs 0.000 description 1
- IYIKLHRQXLHMJQ-UHFFFAOYSA-N amiodarone Chemical compound CCCCC=1OC2=CC=CC=C2C=1C(=O)C1=CC(I)=C(OCCN(CC)CC)C(I)=C1 IYIKLHRQXLHMJQ-UHFFFAOYSA-N 0.000 description 1
- 229960005260 amiodarone Drugs 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 229940044094 angiotensin-converting-enzyme inhibitor Drugs 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 206010003441 asbestosis Diseases 0.000 description 1
- 201000009408 aspiration pneumonitis Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 230000003376 axonal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008236 biological pathway Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 208000015440 bird fancier lung Diseases 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000004856 capillary permeability Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- FFGPTBGBLSHEPO-UHFFFAOYSA-N carbamazepine Chemical compound C1=CC2=CC=CC=C2N(C(=O)N)C2=CC=CC=C21 FFGPTBGBLSHEPO-UHFFFAOYSA-N 0.000 description 1
- 229960000623 carbamazepine Drugs 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229940124587 cephalosporin Drugs 0.000 description 1
- 150000001780 cephalosporins Chemical class 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960001761 chlorpropamide Drugs 0.000 description 1
- 208000016532 chronic granulomatous disease Diseases 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 208000018631 connective tissue disease Diseases 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229940039231 contrast media Drugs 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 239000002254 cytotoxic agent Substances 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 206010013023 diphtheria Diseases 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 239000008298 dragée Substances 0.000 description 1
- 239000000890 drug combination Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000013764 eosinophil chemotaxis Effects 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 208000022195 farmer lung disease Diseases 0.000 description 1
- 230000003176 fibrotic effect Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 208000030304 gastrointestinal bleeding Diseases 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 229950002932 hexamethonium Drugs 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 229960002474 hydralazine Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229960000598 infliximab Drugs 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 208000036971 interstitial lung disease 2 Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 201000009085 invasive aspergillosis Diseases 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 229960002358 iodine Drugs 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 229960003350 isoniazid Drugs 0.000 description 1
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 150000002617 leukotrienes Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 125000000311 mannosyl group Chemical group C1([C@@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 201000008350 membranous glomerulonephritis Diseases 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 206010065579 multifocal motor neuropathy Diseases 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000032027 negative regulation of neutrophil apoptotic process Effects 0.000 description 1
- 230000011242 neutrophil chemotaxis Effects 0.000 description 1
- NXFQHRVNIOXGAQ-YCRREMRBSA-N nitrofurantoin Chemical compound O1C([N+](=O)[O-])=CC=C1\C=N\N1C(=O)NC(=O)C1 NXFQHRVNIOXGAQ-YCRREMRBSA-N 0.000 description 1
- 229960000564 nitrofurantoin Drugs 0.000 description 1
- 239000000041 non-steroidal anti-inflammatory agent Substances 0.000 description 1
- 229940021182 non-steroidal anti-inflammatory drug Drugs 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000006179 pH buffering agent Substances 0.000 description 1
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 150000002960 penicillins Chemical class 0.000 description 1
- 229960002036 phenytoin Drugs 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 230000010118 platelet activation Effects 0.000 description 1
- 206010035653 pneumoconiosis Diseases 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 201000006292 polyarteritis nodosa Diseases 0.000 description 1
- 208000005987 polymyositis Diseases 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 208000000813 polyradiculoneuropathy Diseases 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- REQCZEXYDRLIBE-UHFFFAOYSA-N procainamide Chemical compound CCN(CC)CCNC(=O)C1=CC=C(N)C=C1 REQCZEXYDRLIBE-UHFFFAOYSA-N 0.000 description 1
- 229960000244 procainamide Drugs 0.000 description 1
- 201000008171 proliferative glomerulonephritis Diseases 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 201000003456 pulmonary hemosiderosis Diseases 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 206010039083 rhinitis Diseases 0.000 description 1
- 229960004641 rituximab Drugs 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 230000021595 spermatogenesis Effects 0.000 description 1
- 238000011476 stem cell transplantation Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229940120904 succinylcholine chloride Drugs 0.000 description 1
- YOEWQQVKRJEPAE-UHFFFAOYSA-L succinylcholine chloride (anhydrous) Chemical compound [Cl-].[Cl-].C[N+](C)(C)CCOC(=O)CCC(=O)OCC[N+](C)(C)C YOEWQQVKRJEPAE-UHFFFAOYSA-L 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- UJMBCXLDXJUMFB-GLCFPVLVSA-K tartrazine Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)C1=NN(C=2C=CC(=CC=2)S([O-])(=O)=O)C(=O)C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 UJMBCXLDXJUMFB-GLCFPVLVSA-K 0.000 description 1
- 229960000943 tartrazine Drugs 0.000 description 1
- 235000012756 tartrazine Nutrition 0.000 description 1
- 239000004149 tartrazine Substances 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229940040944 tetracyclines Drugs 0.000 description 1
- 206010043554 thrombocytopenia Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 208000009174 transverse myelitis Diseases 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 238000002689 xenotransplantation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1767—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
Abstract
The present invention relates to methods of treating or preventing a complement-mediated disease and/or disorder in a subject with a complement C5 polymorphism, including administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent that a) inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway; and/or b) inhibits eicosanoid activity. The invention also relates to methods of identifying patient populations with C5 polymorphisms that are treatable with specific agents that a) inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway; and/or b) inhibit eicosanoid activity.
Description
The present invention relates to methods of treating or preventing a complement-mediated disease and/or disorder in a subject with a complement C5 polymorphism, including administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent that a) inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway; and/or b) inhibits eicosanoid activity. The invention also relates to methods of identifying patient populations with C5 polymorphisms that are treatable with specific agents that a) inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway; and/or b) inhibit eicosanoid activity.
NZ 727053 B2 ORNITHODOROS MOUBATA COMPLEMENT INHIBITOR FOR USE IN THE TREATMENT OF COMPLEMENT-MEDIATED DISEASES IN PATIENTS WITH CS POLYMORPHISM FIELD OF THE INVENTION The present invention relates to methods of treating and preventing complement-mediated diseases and disorders in subjects with complement CS polymorphisms.
S All documents mentioned in the text and listed at the end of this description are incorporated herein by reference.
BACKGROUND TO THE INVENTION Polymorphisms are common in all but the most conserved genes in most species. The presence of polymorphisms in genes that are involved in diseases and disorders of human health has led to the advent of personalised medicine. Personalised medicine allows healthcare to be customised to the individual using a variety of tools including molecular genetic analysis. Medical decisions, choice of drugs and/or treatment regimens can be tailored to the individual patient. Diagnostic testing and genotyping can be used to select appropriate and optimal therapies based on the subject's individual responsiveness to IS particular drugs.
It has recently come to light that certain genetic variants m human CS, or CS polymorphisms, give rise to a lack of response to certain agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway. In one clinical trial of eculizumab in a particular Japanese population of patients with the complement-mediated disorder paroxysmal nocturnal haemoglobinuria (PNH), several patients had a poor response. These patients displayed the CS polymorphisms c.26S3C>T (p.Arg88SCys) or c.26S4G>A (p.Arg88SHis).In this type of situation, a sub population of patients may be identified who cannot be treated by conventional means, or perhaps cannot be treated at all if there is no alternative drug available, or if all known 2S drugs act by the same mechanism.
In the present case, there is no available alternative treatment for complement-mediated diseases and disorders that are currently treated using eculizumab. There is therefore a need to identify a means of treating the patient sub-population with CS polymorphisms that render them currently untreatable.
SUMMARY OF THE INVENTION Surprisingly, the present inventors have found that the tick protein Coversin (also referred to as EVS76 and OmCI in the art and herein [2S]) can be used to treat and prevent complement-mediated diseases and disorders in subjects with complement CS S polymorphisms.
Accordingly, the invention provides a method of treating or preventing a complement mediated disease and/or disorder comprising administering to a subject with a complement CS polymorphism and in need thereof a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway.
The invention also provides an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for treating or preventing a complement-mediated disease and/or disorder in a subject with a complement CS polymorphism.
IS The invention also provides a method of treating or preventing a complement-mediated disease and/or disorder comprising the steps of: a) identifying a subject with a CS polymorphism; and b) identifying an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject; and c) administering to said subject a therapeutically or prophylactically effective amount of said agent identified in step (b ).
The invention also provides an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for use in a method of treating or preventing a complement-mediated disease and/or disorder, wherein said 2S method of treating or preventing comprising the steps of: a) identifying a subject with a CS polymorphism; and b) identifying an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject; and c) administering to said subject a therapeutically or prophylactically effective amount of said agent identified in step (b ).
In a further embodiment, the invention provides a method of selecting a subject with a complement-mediated disease or disorder for treatment with a first agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with a C5 polymorphism, comprising determining the effectiveness of a second agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject, wherein a subject is selected for treatment with the first agent if the second agent shows decreased effectiveness in the subject with a C5 polymorphism.
In yet a further embodiment, the invention provides an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for treating a complement-mediated disease or disorder, wherein said agent is administered to a subject on the basis the subject having been determined to have a C5 polymorphism.
Complement The complement system is an essential part of the body's natural defence mechanism against foreign invasion and is also involved in the inflammatory process. More than 30 proteins in serum and at the cell surface are involved in complement system function and regulation. Recently it has become apparent that, as well as the ~35 known components of the complement system which may be associated with both beneficial and pathological processes, the complement system itself interacts with at least 85 biological pathways with functions as diverse as angiogenesis, platelet activation, glucose metabolism and spermatogenesis The complement system is activated by the presence of foreign antigens. Three activation pathways exist: (1) the classical pathway which is activated by IgM and IgG complexes or by recognition of carbohydrates; (2) the alternative pathway which is activated by non-self surfaces (lacking specific regulatory molecules) and by bacterial endotoxins; and (3) the lectin pathway which is activated by binding of manna-binding lectin (MBL) to mannose residues on the surface of a pathogen. The three pathways comprise parallel cascades of events that result in the production of complement activation through the formation of similar C3 and C5 convertases on cell surfaces resulting in the release of acute mediators of inflammation (C3a and C5a) and formation of the membrane attack complex (MAC).
The parallel cascades involved in the classical and alternative pathways are shown in Figure 1.
The classical complement pathway, the alternative complement pathway and the lectin complement pathway are herein collectively referred to as the complement pathways.
S Complement CS polymorphisms Several polymorphisms of human CS have been reported [1-S]. Mutations in the gene encoding CS have been associated with various pathologies including complement component S deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis. Polymorphisms in human CS include insertions, deletions, single amino acid substitutions, frame-shifts, truncations and combinations of these changes.
Certain polymorphisms alter the interaction of CS with inhibitors of complement pathway activation. Certain other polymorphisms alter CS activity with clinical significance.
Polymorphisms affecting Arg88S of wildtype CS are of interest. Two polymorphisms of lS particular interest are Arg88SCys (encoded by c.26S3C>T) and p.Arg88SHis (encoded by c.26S4G>A), both of which decrease the effectiveness of the mAb eculizumab [4].
The term "CS polymorphism" is used herein to mean any variant of CS other than the wild type CS. In a human subject, the wild-type CS is the CS protein with accession number NP _001726.2 ; version GI:38016947. The term "CS polymorphism" includes insertions, deletions, single or multiple amino acid substitutions, frame-shifts, truncations and combinations of these changes in the CS protein.
These polymorphisms can be present as either heterozygous or homozygous polymorphisms, such as heterozygous CS for a given polymorphism, homozygous for one polymorphism or heterozygous for different polymorphisms. 2S Polymorphisms of interest include changes to the amino acid sequence of wildtype CS which are in proximity to, or within the epitope for eculizumab, (i.e. 879KSSKC883, including K879, S880, S881, K882 and/or C883). For example, any change may be in the epitope for eculizumab or up to 10, 9, 8, 7, 6, S, 4, 3, 2, 1 amino acids to the N- or C terminus of the epitope for eculizumab.
Preferably, the amino acid change is not within or in proximity to the Coversin binding site of CS. This is believed to be a conserved region atop CS a at the distal end of the highly conserved CUB-CSd-MG8 superdomain of CS.
Of particular interest in the present invention are CS polymorphisms that decrease the S effectiveness of one or more agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with wild-type CS. By "decrease the effectiveness" it is meant that the agent has an IC for the polymorphic CS protein that is at least 2, 3, 4, S, 6, 7, 8, 9, 10, 20, 30, 40, SO, 100, 1000 or more times greater than the IC of the same agent for the wild-type CS protein.
In a preferred embodiment, the CS polymorphism decreases the effectiveness of one or more agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway, but does not decrease the effectiveness of Coversin or functional equivalents thereof. In a further preferred embodiment, the CS polymorphism decreases the effectiveness of one or more anti-CS monoclonal antibodies IS that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with wild-type CS, but does not decrease the effectiveness of other agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by binding to CS without blocking the CS convertase binding site.
By "does not decrease the effectiveness" it is meant that the IC of Coversin or other agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by binding to CS without blocking the CS convertase binding site, for the wild-type CS protein is at least 7S%, 80%, 8S%, 90%, 9S%, 96%, 97%, 98%, 99% or 100% of the IC of Coversin or other agents that inhibit the classical 2S complement pathway, the alternative complement pathway and the lectin complement pathway by binding to CS without blocking the CS convertase binding site for the polymorphic CS protein. The term "does not decrease" also encompasses an increase in effectiveness.
In an alternative embodiment, effectiveness can be measured by measuring the ability of the agent to inhibit complement activation in serum taken from the subject. For example, complement activity in the serum of said subjects can be measured by any means known in the art or described herein, for example the haemolytic assays described in reference [ 6].
An agent would be considered to inhibit complement activity in said subject if complement activity in the presence of the agent is reduced when compared to a control. By "reduced" in this context it is meant that complement activity in the treated sample is at least 10, 20, , 40, SO, 60, 70, 80, 90, or 100%, reduced compared to a control.
S In a particular embodiment, the CS polymorphism decreases the effectiveness of monoclonal antibody agents in inhibiting activation of one or more of the complement pathways. In a particular embodiment, the CS polymorphism decreases the effectiveness of the monoclonal antibody eculizumab in inhibiting activation of one or more of the complement pathways. In a further embodiment, the CS polymorphism decreases the effectiveness of agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by blocking the CS convertase binding site. In a further specific embodiment, the CS polymorphism is at position Arg88S. Specific polymorphisms at this position include Arg88SCys or Arg88SHis.
Polymorphisms that alter binding the affinity of CS to known anti-CS monoclonal IS antibodies such as eculizumab, Pexelizumab, and/or N19-8, or the effectiveness of peptidic complement inhibitors such as ARC190S are also of interest in the context of this invention.
Thus, in a specific embodiment the invention provides a method of treating or preventing a complement-mediated disease and/or disorder comprising administering to a subject with a complement CS polymorphism and in need thereof, a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway, wherein the complement CS polymorphism decreases the effectiveness of agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by 2S blocking the CS convertase binding site, but does not decrease the effectiveness of agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway without blocking the CS convertase binding site.
Thus, in a specific embodiment the invention provides a method of treating or preventing a complement-mediated disease and/or disorder comprising administering to a subject with a complement CS polymorphism and in need thereof, a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway, wherein the complement CS polymorphism decreases the effectiveness of monoclonal antibodies that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway but does not decrease the effectiveness of Coversin or functional equivalents of this agent.
S In this specific embodiment, the invention also provides a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for treating or preventing a complement-mediated disease and/or disorder in a subject with a complement CS polymorphism, wherein the complement CS polymorphism decreases the effectiveness of agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by blocking the CS binding site, but does not decrease the effectiveness of agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway without blocking the CS binding site. l S In this specific embodiment, the invention also provides a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for treating or preventing a complement-mediated disease and/or disorder in a subject with a complement CS polymorphism, wherein the complement CS polymorphism decreases the effectiveness of monoclonal antibodies that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway but does not decrease the effectiveness of Coversin or functional equivalents of this agent.
Thus, in a further specific embodiment the invention provides a method of treating or preventing a complement-mediated disease and/or disorder comprising administering to a 2S subject with a complement CS polymorphism and in need thereof a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway, wherein the complement CS polymorphism is at position Arg88S and wherein the agent is a protein comprising or consisting of amino acids 19 to 168 of the amino acid sequence in SEQ ID NO: 2 or is a functional equivalent of this protein.
In this specific embodiment, the invention also provides a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for treating or preventing a complement-mediated disease and/or disorder in a subject with a complement CS polymorphism wherein the complement CS polymorphism is at position Arg 88S and wherein the agent is a protein comprising or consisting of amino acids 19 to S 168 of the amino acid sequence in SEQ ID NO: 2 or is a functional equivalent of this protein.
Identifying subjects for treatment The present invention is particularly useful m subjects that have a polymorphism in complement CS. The subject may either be already known to have a CS polymorphism, or may be suspected of having a CS polymorphism. A subject may be suspected of having a polymorphism in CS for example because of clinical signs of a complement-mediated disorder, because of ethnic origin or pedigree with an incidence of CS polymorphisms, or because of unexpectedly poor response, and/or unexpectedly high response, to an agent that inhibits one of the complement pathways. lS The invention may be useful in the sub-population of subjects that have an unexpectedly poor response to one or more agents that inhibit one of the complement pathways. In particular, the invention is useful in sub-population of subjects with a CS polymorphism that decreases the effectiveness of monoclonal antibody agents in inhibiting activation of one or more of the complement pathways. In a particular embodiment, the CS polymorphism decreases the effectiveness of the monoclonal antibody eculizumab in inhibiting activation of one or more of the complement pathways.
For example, subjects with two CS polymorphisms at position Arg88S (c.26S3C>T (p.Arg88SCys) and c.26S4G>A (p.Arg88SHis)) do not respond to eculizumab. However, Coversin has been shown to be able to inhibit CS cleavage and activation of the 2S complement pathways even in these subjects. Coversin interacts with complement CS protein in a different manner to the known anti-CS mAbs, and it is therefore expected that Coversin will also be useful in sub-populations of subjects that are not responsive to known anti-CS mAbs, and in subjects that have other CS polymorphisms. Coversin binds to CS, which results in stabilization of the global conformation of CS but does not block the CS convertase cleavage site [7]. In contrast, eculizumab blocks the CS convertase binding site [8].
The polymorphisms Arg88SCys and Arg88SHis are particularly prevalent in subjects of Japanese and Han Chinese origin. Coversin is therefore a particularly advantageous choice of agent in a sub-population with these ethnic origins.
As can be seen from the Examples, these polymorphisms are not limited to subjects of S Japanese and Han Chinese origin. Subjects with CS polymorphisms can also be identified by other routine techniques including molecular genetic analysis of the gene encoding the CS protein including sequencing of the gene [4]; testing the ability of various agent to inhibit complement activation in the subject as described herein or by other methods known in the art; and/or biochemical analysis of the CS protein from the subject, including isoelectric focusing and functional detection [9]. In a clinical setting, a subject with a CS polymorphism may be identified by an unexpectedly poor response to an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway.
It is also anticipated that Coversin will be useful in sub-populations of subject that are IS unexpectedly sensitive to other agents that inhibit one of the complement pathways. For example, if a polymorphism increases the affinity of another agent, such as eculizumab, for the CS protein, it may be difficult to dose the agent correctly. Activation of complement must be tightly controlled to prevent damage to the body's own tissues, and therefore Coversin would be a more attractive alternative in this scenario.
Once a subject with a CS polymorphism has been identified, it is possible to identify an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject. In order to identify an agent that inhibits the complement pathways, complement activity in the serum of the subject is assessed in the presence and absence of a variety of agents that inhibit the classical 2S complement pathway, the alternative complement pathway and the lectin complement pathway, as described herein. In one specific embodiment, the agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject is Coversin or a functional equivalent thereof.
Complement activity in the serum of said subjects can be measured by any means known in the art or described herein, for example the haemolytic assays described in reference and/or by using the Quidel CHSO method as referred to in the examples An agent would be considered to inhibit complement activity in said subject if complement activity in the presence of the agent is reduced when compared to a control. By "reduced" in this context it is meant that complement activity in the treated sample is at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 100%, reduced compared to a control.
The invention therefore provides a method of treating or preventing a complement- mediated disease and/or disorder comprising the steps of: a) identifying a subject with a C5 polymorphism; and b) identifying an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject; and c) administering to said subject a therapeutically or prophylactically effective amount of said agent identified in step (b ).
The invention also provides a therapeutically or prophylactically effective amount of an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for use in a method of treating or preventing a complement-mediated disease and/or disorder, wherein said method of treating or preventing comprising the steps of: a) identifying a subject with a C5 polymorphism; and b) identifying an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject; and c) administering to said subject a therapeutically or prophylactically effective amount of said agent identified in step (b ).
In yet a further embodiment, the invention provides an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with a C5 polymorphism for treating a complement-mediated disease or disorder, wherein said agent is administered to a subject on the basis of the subject having been determined to have a C5 polymorphism.
In a further specific embodiment, the invention provides agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with a C5 polymorphism for treating a complement-mediated disease or disorder in a subject, wherein a) a biological sample from said subject is assayed for the presence or absence of a C5 polymorphism, and b) a therapeutically effective amount of said agent is selectively administered to the individual on the basis of the presence of the CS polymorphism.
In a specific embodiment, the subject with a CS polymorphism is identified by a lack of response to a monoclonal antibody that inhibits the classical complement pathway, the S alternative complement pathway and the lectin complement pathway in wild-type subjects.
This sub-population of subjects is referred to as "non-responders". Non-responders can be identified by confirming that serum complement activity is at least 60% of normal serum complement activity in the presence of the monoclonal antibody that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with wild-type CS.
Of particular interest in the present invention are subjects that are non-responders to eculizumab, Pexelizumab, N19-8 and/or ARC109S.
In further specific embodiments, the specific CS polymorphism may be identified or confirmed by sequencing the gene encoding CS or by other molecular genetic analysis.
IS In a further embodiment, the invention provides a method of selecting a subject with a complement-mediated disease or disorder for treatment with a first agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with a CS polymorphism, comprising determining the effectiveness in said subject of a second agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a wild-type subject, wherein a subject is selected for treatment if the second agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a wild-type subject shows decreased effectiveness in the subject with a CS polymorphism. 2S Inhibition of the classical complement pathway, the alternative complement pathway and the lectin complement pathway in said subject can be measured by measuring the ability of an agent to prevent complement activation in serum from the subject, as described herein.
In a specific embodiment, the invention provides an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway for treating a complement-mediated disease or disorder, wherein said agent is administered to a subject on the basis of a sample from the subject having been determined to have at least 60% of normal serum complement activity in the presence of an anti-CS monoclonal antibody that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in subjects with wild-type CS.
In a further specific embodiment, the invention provides an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement S pathway for treating a complement-mediated disease or disorder in a subject, wherein a) a biological sample from said subject is assayed for the presence or absence of at least 60% of normal serum complement activity in the presence of an anti-CS monoclonal antibody that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in subjects with wild-type CS, and b) a therapeutically effective amount of said agent is selectively administered to the individual on the basis of the presence of at least 60% of normal serum complement activity in the presence of an anti-CS monoclonal antibody that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway in subjects with wild-type CS. lS By "at least 60% of normal serum complement activity in the presence of an anti-CS monoclonal antibody" it is meant that the serum complement activity of the subject is at least 60%, at least 70%, at least 80%, at least 90%, at least 9S% or more of the serum complement activity of a normal, untreated control subject. The control subject may have wild-type CS or may be the same subject prior to treatment with the anti-CS monoclonal antibody.
In some embodiments, the anti-CS monoclonal antibody 1s eculizumab, Pexelizumab and/or N19-8.
These methods can be used to identify subjects and populations of subjects who are susceptible to treatment with Coversin and functional equivalents thereof. 2S Complement-mediated diseases and disorders Activation of complement must be tightly controlled to prevent damage to the body's own tissues. Failure to control complement activation has been shown to play a role in a variety of diseases including, amongst others, acute pancreatitis, Age Related Macular Degeneration (AMD), atypical haemolytic uremeic syndrome (aHUS), Alzheimer's disease, Huntingdon's disease, Parkinson's disease, allergic encephalomyelitis, allotransplatation, asthma, adult respiratory distress syndrome, influenza, bum injuries, Crohn's disease, glomerulonephritis, haemolytic anaemia, haemodialysis, hereditary angioedema, ischaemia reperfusion injuries, multiple system organ failure, multiple sclerosis, myasthenia gravis, myocardial infarction, paroxysmal nocturnal haemoglo binuria (PNH), psoriasis, rheumatoid arthritis, septic shock, systemic lupus erythematosus, stroke, thrombotic thrombocytopaenicpurpura (TTP), traumatic brain injury, vascular leak syndrome, and transplantation rejection and graft versus host disease (GvHD), as well as various other peripheral nerve disorders and respiratory disorders [11-16].
Peripheral nerve disorders as listed in reference 15 include of post-infective demyelinating polyradiculoneuropathy (Guillain Barre syndrome), Miller Fisher syndrome, acute inflammatory demyelinating polyradiculoneuropathy (AIDP), chronic inflammatory demyelinating polyradiculoneuropathy (CIDP), diabetic neuropathy, uraemic pruritus, multifocal motor neuropathy, paraproteinaemic neuropathy, anti-Hu neuropathy, post diphtheria demyelinating neuropathy, multiple sclerosis, radiation myelopathy, giant cell arteritis (temporal arteritis), transverse myelitis, motor neurone disease, dermatomyositis.
Respiratory disorders as listed in reference 14 include asthma, including severe and steroid resistant asthma, COPD, immune complex alveolitis including those caused by exposure to organic dusts, moulds, airborne allergens, mineral dust, chemicals etc. Further conditions included in the definition of respiratory disorders include: farmer's lung, pigeon or bird fancier's lung, barn fever, miller's lung, metalworker's lung, humidifier fever, silicosis, pneumoconiosis, asbestosis, byssinosis, berylliosis, mesothelioma, rhinitis, alveolitis or diffuse fibrotic lung disease caused by exposure to systemic or inhaled drugs and chemical agents including but not limited to: bleomycin, mitomycin, penicillins, sulphonamides, cephalosporins, aspirin, NSAIDs, tartrazine, ACE inhibitors, iodine containing contrast media, non-selective ~ blocking drugs, suxamethonium, hexamethonium, thiopentone, amiodarone, nitrofurantoin, paraquat, oxygen, cytotoxic agents, tetracyclines, phenytoin, carbamazepine, chlorpropamide, hydralazine, procainamide, isoniazid, p-aminosalicylic acid. Furthermore, the term includes physical lung damage including but not limited to: crush injury, smoke and hot gas inhalation, blast injury, radiation injury, aspiration pneumonitis, lipoid pneumonia; lung damage associated with organ transplantation including but not limited to: cardiac transplantation, lung transplantation, bone marrow transplantation. Also included within the definition of respiratory disorder are cryptogenic fibrosing alveolitis, allergic granulomatosis (Churg-Strauss syndrome), wegener's granulomatosis, broncheolitis obliterans, interstitial pulmonary fibrosis, cystic fibrosis.
Also included are respiratory manifestations of autoimmune and connective tissue diseases including but not limited to: rheumatoid disease, systemic lupus erythematosus, systemic sclerosis, polyarteritis nodosa, polymyositis, dermatomyositis, sj6gren's syndrome, ankylosing spondylitis, caplan's syndrome, goodpasture's syndrome, pulmonary alveolar S proteinosis, idiopathic pulmonary haemosiderosis, histiocytosis X, pulmonary infiltration with eosinophilia (PIE) including but not limited to: simple pulmonary eosinophilia, prolonged pulmonary eosinophilia, asthmatic bronchopulmonary eosinophilia, allergic bronchopulmonary aspergillosis, aspergilloma, invasive aspergillosis, tropical pulmonary eosinophilia, hypereosinohilic syndrome, parasitic infestation and lymphangioleiomyomatosis (LAM).
Of particular interest in the present invention are paroxysmal nocturnal haemoglobinuria (PNH), graft versus host disease (GvHD), thrombotic thrombocytopaenicpurpura (TTP) and atypical haemolytic uremeic syndrome (aHUS).
Agent to be used in the invention lS In one aspect of the invention, the agent may bind complement CS, including complement CS from subjects with complement CS polymorphisms. The agent may act to prevent the cleavage of complement CS, including complement CS from subjects with complement CS polymorphisms, by CS convertase into complement CSa and complement CSb-9. The agent may act to reduce CSa levels in a subject compared to an untreated subject.
In one aspect of the invention, the agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway binds to CS in such a way as to stabilize the global conformation of CS but not block the CS convertase cleavage site. Binding of Coversin to CS results in stabilization of the global conformation of CS but does not block the convertase cleavage site. 2S The complement CS protein, also referred to herein as CS, is cleaved by the CS convertase enzyme, itself formed from C3a, an earlier product of the alternative pathway (Figure 1 ).
The products of this cleavage include an anaphylatoxin CS a and a lytic complex CSb - 9 also known as membrane attack complex (MAC). CSa is a highly reactive peptide implicated in many pathological inflammatory processes including neutrophil and eosinophil chemotaxis, neutrophil activation, increased capillary permeability and inhibition of neutrophil apoptosis [17].
MAC is associated with other important pathological processes including rheumatoid arthritis [ 18; 19], proliferative glomerulonephritis [20], idiopathic membranous nephropathy [21], proteinurea [22], demyelination after acute axonal injury [23] and is also responsible for acute graft rejection following xenotransplantation [24].
S Monoclonal antibodies and small molecules that bind and inhibit CS have been developed to treat various diseases [12], in particular PNH, psoraiasis, rheumatoid arthritis, systemic lupus erythematosus and transplant rejection. However, these monoclonal antibodies do not bind to certain CS proteins from subjects with CS polymorphisms, and are thus ineffective in these subjects [ 4].
In contrast, the Coversin, and functional equivalents thereof, inhibit complement CS cleavage both in subjects with wild-type CS and in subjects with CS polymorphisms.
The ability of an agent to bind CS, including CS from subjects with CS polymorphisms, may be determined by standard in vitro assays known in the art, for example by western blotting following incubation of the protein on the gel with labelled CS. Preferably, the IS agent according to the invention binds CS, either wild-type and/or CS from subjects with CS polymorphisms, with an IC of less than 0.2 mg/ml, preferably less than 0.1 mg/ml, preferably less than O.OS mg/ml, preferably less than 0.04 mg/ml, preferably less than 0.03 mg/ml, preferably 0.02 mg/ml, preferably less than lµg/ml, preferably less than lOOng/ml, preferably less than lOng/ml, more preferably still, less than Ing/ml. The agent need not have the same affinity for wild-type CS and CS from subjects with CS polymorphisms. It may show higher, lower or the same affinity for wild-type CS and CS from subjects with CS polymorphisms.
The ability of an agent to inhbit complement activation may be determined by measuring the ability of the agent to inhibit complement activation in serum. For example, 2S complement activity in the serum can be measured by any means known in the art or described herein.
According to one embodiment of the invention, the agent that binds CS is not an anti-CS monoclonal antibody.
The invention also provides a method of treating or preventing a complement-mediated disease and/or disorder in a subject with a complement CS polymorphism comprising administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent that inhibits eicosanoid activity.
The invention also provides a therapeutically or prophylactically effective amount of an agent that inhibits eicosanoid activity for treating or preventing a complement-mediated S disease and/or disorder in a subject with a complement CS polymorphism.
The agent according to this aspect of the invention may inhibit leukotrine B4 (LTB4) activity. In particular, the agent according to this aspect of the invention may bind LTB4.
The ability of an agent to bind L TB4 may be determined by standard in vitro assays known in the art, for example by western blotting following incubation of the protein on the gel with labelled LTB4. The agent according to the invention may bind LTB4 with an IC of less than 0.2 mg/ml, preferably less than 0.1 mg/ml, preferably less than O.OS mg/ml, preferably less than 0.04 mg/ml, preferably less than 0.03 mg/ml, preferably 0.02 mg/ml, preferably less than 1 µg/ml, preferably less than 1 OOng/ml, preferably less than 1 Ong/ml, more preferably still, less than Ing/ml.
IS In one aspect, the invention provides a method of treating or preventing a complement mediated disease and/or disorder in a subject with a complement CS polymorphism comprising administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent that: a) inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway; and/or b) inhibits eicosanoid activity.
The invention also provides an agent that inhibits: a) the classical complement pathway, the alternative complement pathway and the lectin complement pathway; and/or 2S b) eicosanoid activity, for treating or preventing a complement-mediated disease and/or disorder in a subject with a complement CS polymorphism.
According to one embodiment of this aspect of the invention, the agent binds all of CS, CS from subjects with CS polymorphisms, and LTB4. The agent according to this embodiment may thus act to prevent the cleavage of complement CS by CS convertase into complement CSa and complement CSb-9 (MAC), and also to inhibit LTB4 activity. Using an agent which binds to both CS and L TB4 is particularly advantageous. CS and the eicosanoid pathway are both believed to contribute to the observed pathology in many complement mediated diseases and disorders. Thus by using a single agent which inhibits multiple S pathways involved in the inflammatory effects of complement-mediated diseases and disorders, an enhanced effect can be achieved, compared to using an agent which inhibits only a single pathway involved in the inflammatory effects of complement-mediated diseases and disorders. There are furthermore practical advantages associated with administering a single molecule.
Preferably, the agent of the invention is derived from a haematophagous arthropod. The term "haematophagous arthropod" includes all arthropods that take a blood meal from a suitable host, such as insects, ticks, lice, fleas and mites. Preferably, the agent is derived from a tick, preferably from the tick Ornithodoros moubata.
According to one embodiment of the invention, the agent is a protein comprising amino lS acids 19 to 168 of the amino acid sequence in Figure 2 (SEQ ID NO: 2) or is a functional equivalent of this protein. The agent may be a protein consisting of amino acids 19 to 168 of the amino acid sequence in Figure 2 or be a functional equivalent of this protein.
According to an alternative embodiment, the protein used according to this embodiment of the invention may comprise or consist of amino acids 1 to 168 of the amino acid sequence in Figure 2 (SEQ ID NO: 2), or be a functional equivalent thereof. The first 18 amino acids of the protein sequence given in Figure 2 form a signal sequence which is not required for CS binding or for L TB4 binding activity and so this may optionally be dispensed with, for example, for efficiency of recombinant protein production.
The protein having the amino acid sequence given in Figure 2, also referred to herein as 2S the Coversin protein, was isolated from the salivary glands of the tick Ornithodoros moubata. Coversin is an outlying member of the lipocalin family and is the first lipocalin family member shown to inhibit complement activation. The Coversin protein inhibits the alternative, classical and lectin complement pathways by binding CS and preventing its cleavage by CS convertase into Complement CSa and Complement CSb - 9, thus inhibiting both the action of CS a peptide and the MAC. The Coversin protein also binds LTB4. The term "Coversin protein", as used herein, refers to the sequence given in Figure 2 with or without the signal sequence.
The Coversin protein and the ability of this protein to inhibit complement activation has been disclosed in [25], where the Coversin protein was referred to as the "OmCI protein".
The Coversin protein has also been shown to be effective in the treatment of myasthenia gravis [13], respiratory disorders [14] and peripheral nerve disorders [15]. The ability of the Coversin protein to bind eicosanoids including L TB4 and its use in the treatment of diseases mediated by a leukotriene or hydroxyeicosanoid has been suggested in [26]. None of these disclosures suggest that the Coversin protein could be useful in the treatment or prevention of complement-mediated disorders in subjects with a C5 polymorphism.
It has now been found that the Coversin protein is surprisingly effective in the treatment and prevention of complement-mediated disorders in subjects with a C5 polymorphism.
The data presented herein demonstrate that, in a subject with an Arg885His polymorphism, inhibition of complement activity in vitro was resistant to eculizumab (30% or 30-80% complement inhibition at best) but completely sensitive to Coversin, with 100% inhibition at all concentrations tested.
According to a further embodiment of the invention, the agent may be a nucleic acid molecule encoding the Coversin protein or a functional equivalent thereof. For example, gene therapy may be employed to effect the endogenous production of the Coversin protein by the relevant cells in the subject, either in vivo or ex vivo. Another approach is the administration of "naked DNA" in which the therapeutic gene is directly injected into the bloodstream or into muscle tissue.
Preferably, such a nucleic acid molecule comprises or consists of bases 55 to 507 of the nucleotide sequence in Figure 2 (SEQ ID NO: 1 ). This nucleotide sequence encodes the Coversin protein in Figure 2 without the signal sequence. The first S4 bases of the nucleotide sequence in Figure 2 encode the signal sequence which is not required for complement inhibitory activity or LTB4 binding activity. Alternatively, the nucleic acid molecule may comprise or consist of bases 1 to S07 of the nucleic acid sequence in Figure S 2, which encodes the protein with the signal sequence.
The Coversin protein has been demonstrated to bind to CS and prevent its cleavage by CS convertase in rat, mouse and human serum with an IC of approximately 0.02mg/ml.
Preferably, functional equivalents of the Coversin protein which retain the ability to bind CS with an IC ofless than 0.2 mg/ml, preferably less than 0.1 mg/ml, preferably less than O.OS mg/ml, preferably less than 0.02 mg/ml, preferably less than lµg/ml, preferably less than lOOng/ml, preferably less than I Ong/ml, more preferably still, less than Ing/ml.
The Coversin protein has also been demonstrated to bind LTB4. Functional equivalents of the Coversin protein may also retain the ability to bind L TB4 with a similar affinity as the Coversin protein.
IS In one respect, the term "functional equivalent" is used herein to describe homologues and fragments of the Coversin protein which: a) retain its ability to bind CS, either wild-type CS or CS from a subject with a CS polymorphism, and to prevent the cleavage of complement CS by CS convertase into complement CS a and complement CSb-9; and/orb) retain its ability to bind LTB4.
The term "functional equivalent" also refers to molecules that are structurally similar to the Coversin protein or that contain similar or identical tertiary structure, particularly in the environment of the active site or active sites of the Coversin protein that binds to CS, either wild-type CS or CS from a subject with a CS polymorphism, and/or LTB4, such as synthetic molecules. Amino acids in Coversin that are likely to be required for LTB4 2S binding are described in [26].
The term "homologue" is meant to include reference to paralogues and orthologues of the Coversin sequence that is explicitly identified in Figure 2, including, for example, the Coversin protein sequence from other tick species, including Rhipicephalus appendiculatus, R. sanguineus, R. bursa, A. americanum, A. cajennense, A. hebraeum, Boophilus microplus, B. annulatus, B. decoloratus, Dermacentor reticulatus, D. andersoni, D. marginatus, D. variabilis, Haemaphysalis inermis, Ha. leachii, Ha. punctata, Hyalomma anatolicum anatolicum, Hy. dromedarii, Hy. marginatum marginatum, Ixodes ricinus, I. persulcatus, I. scapularis, I. hexagonus, Argas persicus, A. reflexus, Ornithodoros erraticus, 0. moubata moubata, 0. m. porcinus, and 0. savignyi. The term "homologue" is also meant to include the equivalent Coversin protein sequence from mosquito species, including those of the Culex, Anopheles and Aedes genera, particularly Culex quinquefasciatus, Aedes aegypti and Anopheles gambiae; flea species, such as Ctenocephalides felis (the cat flea); horseflies; sandflies; blackflies; tsetse flies; lice; mites; leeches; and flatworms. The native Coversin protein is thought to exist in 0. moubata in another three forms of around 18kDa and the term "homologue" is meant to include these alternative forms of Coversin.
Methods for the identification of homologues of the Coversin sequence given in Figure 2 will be clear to those of skill in the art. For example, homologues may be identified by homology searching of sequence databases, both public and private. Conveniently, publicly available databases may be used, although private or commercially-available databases will be equally useful, particularly if they contain data not represented in the public databases. Primary databases are the sites of primary nucleotide or amino acid sequence data deposit and may be publicly or commercially available. Examples of publicly-available pnmary databases include the GenBank database (http://www.ncbi.nlm.nih.gov/), the EMBL database (http://www.ebi.ac.uk/), the DDBJ database (http://www.ddbj.nig.ac.jp/), the SWISS-PROT protein database (http://expasy.hcuge.ch/), PIR (http://pir.georgetown.edu/), TrEMBL (http://www.ebi.ac.uk/), the TIGR databases (see http://www.tigr.org/tdb/index.html), the NRL-3D database (http://www.nbrfa.georgetown.edu), the Protein Data Base (http://www.rcsb.org/pdb), the NRDB database (ftp ://ncbi.nlm.nih.gov/pub/nrdb/README), the OWL database (http://www.biochem.ucl.ac.uk/bsm/dbbrowser/OWL/) and the secondary databases PROSITE (http://expasy.hcuge.ch/sprot/prosite.html), PRINTS (http ://iupab.leeds.ac. uk/bmb5dp/prints.html), Profiles (http ://ulrec3. unil. ch/ so ftware/PFSCAN _form.html), Pfam (http://www.sanger.ac.uk/software/pfam), Identify (http://dna.stanford.edu/identify/) and Blocks (http://www.blocks.fhcrc.org) databases. Examples of commercially-available databases or private databases include PathoGenome (Genome Therapeutics Inc.) and PathoSeq (previously oflncyte Pharmaceuticals Inc.).
Typically, greater than 30% identity between two polypeptides (preferably, over a specified region such as the active site) is considered to be an indication of functional equivalence and thus an indication that two proteins are homologous. Preferably, proteins that are homologues have a degree of sequence identity with the Coversin protein sequence S identified in Figure 2 (SEQ ID N0:2) of greater than 60%. More preferred homologues have degrees of identity of greater than 70%, 80%, 90%, 9S%, 98% or 99%, respectively with the Coversin protein sequence given in Figure 2 (SEQ ID N0:2). Percentage identity, as referred to herein, is as determined using BLAST version 2.1.3 using the default parameters specified by the NCBI (the National Center for Biotechnology Information; http://www.ncbi.nlm.nih.gov/) [Blosum 62 matrix; gap open penalty=l 1 and gap extension penalty= 1].
Functional equivalents of the Coversin protein sequence given in Figure 2 include mutants containing amino acid substitutions, insertions or deletions from the wild type sequence, for example, of 1, 2, 3, 4, S, 7, 10 or more amino acids, provided that such mutants retain the lS ability to bind wild-type CS and/or CS from subjects with a CS polymorphism. Mutants thus include proteins containing conservative amino acid substitutions that do not affect the function or activity of the protein in an adverse manner. This term is also intended to include natural biological variants (e.g. allelic variants or geographical variations within the species from which the Coversin proteins are derived). Mutants with improved ability to bind wild- type CS and/or CS from subjects with a CS polymorphism and/or LTB4 may also be designed through the systematic or directed mutation of specific residues in the protein sequence.
Fragments of the Coversin protein and of homologues of the Coversin protein are also embraced by the term "functional equivalents" providing that such fragments retain the ability to bind wild-type CS and/or CS from subjects with a CS polymorphism and/or LTB4. 2S Fragments may include, for example, polypeptides derived from the Coversin protein sequence which are less than lSO amino acids, less than 12S amino acids, less than 100 amino acids, less than 7S amino acids, less than SO amino acids, or even 2S amino acids or less, provided that these fragments retain the ability to bind to complement wild-type CS and/or CS from subjects with a CS polymorphism and/or LTB4. Fragments may include, for example, polypeptides derived from the Coversin protein sequence which are at least lSO amino acids, at least 12S amino acids, at least 100 amino acids, at least 7S amino acids, at least SO amino acids, or at least 2S amino acids, provided that these fragments retain the ability to bind to complement wild-type CS and/or CS from subjects with a CS polymorphism and/or LTB4.
Any functional equivalent or fragment thereof preferably retains the pattern of cysteine residues that is found in Coversin. For example said functional equivalent comprises six cysteine residues that are spaced relative to each other at a distance of 32 amino acids apart, 62 amino acids apart, 28 amino acids apart, 1 amino acid apart and 21 amino acids S apart as arranged from the amino terminus to the carboxyl terminus of the sequence according to amino acids 1 to 168 of the amino acid sequence in Figure 2 (SEQ ID N0:2).
Exemplary fragments of Coversin protein are disclosed in SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14. The DNA encoding the corresponding fragments are disclosed in SEQ ID NO: 3, SEQ ID NO: S, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13.
Included as such fragments are not only fragments of the 0. moubata Coversin protein that is explicitly identified herein in Figure 2, but also fragments ofhomologues of this protein, as described above. Such fragments of homologues will typically possess greater than 60% identity with fragments of the Coversin protein sequence in Figure 2, although more lS preferred fragments of homologues will display degrees of identity of greater than 70%, 80%, 90%, 9S%, 98% or 99%, respectively with fragments of the Coversin protein sequence in Figure 2. Fragments with improved may, of course, be rationally designed by the systematic mutation or fragmentation of the wild type sequence followed by appropriate activity assays. Fragments may exhibit similar or greater affinity for CS, either the wild-type or polymorphic variant of CS or both, and/or LTB4 as Coversin.
A functional equivalent used according to the invention may be a fusion protein, obtained, for example, by cloning a polynucleotide encoding the Coversin protein in frame to the coding sequences for a heterologous protein sequence. The term "heterologous", when used herein, is intended to designate any polypeptide other than the Coversin protein or its 2S functional equivalent. Example of heterologous sequences, that can be comprised in the soluble fusion proteins either at N- or at C-terminus, are the following: extracellular domains of membrane-bound protein, immunoglobulin constant regions (Fe region), multimerization domains, domains of extracellular proteins, signal sequences, export sequences, or sequences allowing purification by affinity chromatography. Many of these heterologous sequences are commercially available in expression plasmids since these sequences are commonly included in the fusion proteins in order to provide additional properties without significantly impairing the specific biological activity of the protein fused to them [27]. Examples of such additional properties are a longer lasting half-life in body fluids, the extracellular localization, or an easier purification procedure as allowed by a tag such as a histidine, GST, FLAG, avidin or HA tag.
The Coversin protein and functional equivalents thereof, may be prepared in recombinant form by expression in a host cell. Such expression methods are well known to those of skill in the art and are described in detail by [28] and [29]. Recombinant forms of the Coversin protein and functional equivalents thereof are preferably unglycosylated.
The proteins and fragments of the present invention can also be prepared usmg conventional techniques of protein chemistry. For example, protein fragments may be prepared by chemical synthesis. Methods for the generation of fusion proteins are standard in the art and will be known to the skilled reader. For example, most general molecular biology, microbiology recombinant DNA technology and immunological techniques can be found in [28] or [30].
Modes of administration Coversin and its functional equivalents do not reqmre a medical professional for administration to be carried out, and these molecules are rapidly absorbed. Many recombinant antibodies are absorbed very slowly and as a result need to be infused over long periods (e.g. intravenously). The administration of such molecules thus also requires a medical professional. Thus, as well as having the advantage of being more effective at inhibiting the activation of the complement pathways in subjects with a C5 polymorphism, Coversin also possesses the advantage of being easier to administer than other agents such as antibodies like eculizumab.
The subject to which the agent is administered in the practice of the invention is preferably a mammal, preferably a human. The subject may be an adult, a child, or an infant. The subject to which the agent is administered may also be suffering from a complement- mediated disease or disorder. In particular, the subject may be known to have, or be suspected of having, a complement CS polymorphism.
The agent is administered in a therapeutically or prophylactically effective amount. The term "therapeutically effective amount" refers to the amount of agent needed to treat the complement-mediated disease or disorder, as defined elsewhere herein. The term "prophylactically effective amount" used herein refers to the amount of agent needed to prevent complement-mediated disease or disorder as defined elsewhere herein. Preferably, the dose of the agent is sufficient to bind as much available C5 as possible in the subject, more preferably, all available C5. The dose of the agent may alternatively be sufficient to bind as much available LTB4 as possible in the subject, more preferably, all available LTB4. In some aspects, the dose of the agent is sufficient to binds as much available C5 and LTB4 as possible, for example all available C5 and LTB4. The dose of the agent supplied is at least twice the molar dose needed to bind all available C5 and/or L TB4 in the subject. The dose of the agent supplied may be 2.5 times, 3 times or 4 times the molar dose needed to bind all available C5 and/or LTB4 in the subject. Preferably, the dose is from 0.0001 mg/kg (mass of drug compared to mass of patient) to 20 mg/kg, preferably 0.001 mg/kg to 10 mg/kg, preferably 0.01 mg/kg to 2 mg/kg, preferably O.lmg/kg to lmg/kg; alternatively 0.2mg/kg to 0.8mg/kg; alternatively 0.3mg/kg to 0.7mg/kg; alternatively 0.4mg/kg to 0.6mg/kg; for example 0.14mg/kg or 0.57mg/kg. The therapeutically or prophylactically effective amount can additionally be defined in terms of the inhibition of terminal complement, for example, an amount that means that terminal complement activity is reduced by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 91, 92 ,93, 94, 95, 96, 97, 98, 99, 100%, compared to terminal complement activity in the absence of treatment. Dose and frequency may be adjusted in order to maintain terminal complement activity at the desired level, which may be, for example 10% or less, for example 9, 8, 7, 6, 5, 4, 3, 2, 1 % or less compared to terminal complement activity in the absence of treatment.
The frequency with which the dose needs to be administered will depend on the half-life of the agent involved. Where the agent is the Coversin protein or a functional equivalent thereof, the dose may be administered as a continuous infusion, in bolus doses or on a daily basis, twice daily basis, or every two, three, four days, five, six, seven, 10, 15 or 20 days or more. As noted elsewhere, a particular advantage of the Coversin protein and its functional equivalents is the relative ease and rapidity with which it can be administered, and the fact that medical professionals are not required for administration.
Single or multiple doses may be administered. For example at least 2, 3, 4, 5, 6, 7, or 8 doses may be administered. Single doses are one embodiment. The exact dosage and the frequency of doses may also be dependent on the patient's status at the time of administration. Factors that may be taken into consideration when determining dosage include the need for treatment or prophylaxis, the severity of the disease state in the patient, the general health of the patient, the age, weight, gender, diet, time and frequency of administration, drug combinations, reaction sensitivities and the patient's tolerance or response to therapy. The precise amount can be determined by routine experimentation, but may ultimately lie with the judgement of the clinician.
The dosage regimen may also take the form of an initial "loading dose" followed by one or more subsequence "maintenance doses". In general, the loading dose will be greater than the maintenance dose. The loading dose may be 2, 5, 10 or more times greater than the maintenance dose. The loading dose may be administered as a single dose, or as one or more doses in a particular time frame. Typically, the loading dose will be 1, 2, 3, 4 or 5 does administered in a single 24 hour period. The maintenance dose will typically be a lower dose that is repeated at regular intervals, such as every 3, 4, 6, 8, 12, 24, or 48 hours.
The precise regimen can be determined by routine experimentation, but may ultimately lie with the judgement of the clinician.
The loading dose may be O.OOOlmg/kg (mass of drug compared to mass of patient) to 20mg/kg, and the maintenance dose may be between 0.0001 mg/kg to 20mg/kg; alternatively the loading dose is 0.001 mg/kg to 10 mg/kg and the maintenance dose is 0.001 mg/kg to 10 mg/kg, alternatively the loading dose is 0.01 mg/kg to 2 mg/kg and the maintenance dose is O.Olmg/kg to 2mg/kg; alternatively the loading dose is O.lmg/kg to lmg/kg and the maintenance dose is O.lmg/kg to lmg/kg; alternatively the loading dose is O. lmg/kg to lmg/kg and the maintenance dose is 0.05mg/kg to 0.5mg/kg; alternatively the loading dose is 0.2mg/kg to 0.8mg/kg and the maintenance dose is O.lmg/kg to 0.4mg/kg; alternatively the loading dose is 0.3mg/kg to 0.7mg/kg and the maintenance dose is O. lmg/kg to 0.3mg/kg; alternatively the loading dose is 0.4mg/kg to 0.6mg/kg and the maintenance dose is O.lmg/kg to 0.2mg/kg for example where the loading dose is 0.57mg/kg and the maintenance dose is 0.14mg/kg.
The loading dose may be O.OOOlmg/kg (mass of drug compared to mass of patient) to 20mg/kg, and the maintenance dose may be between 0.0001 mg/kg to 20mg/kg; alternatively the maintenance dose may be 0.001 mg/kg to 10 mg/kg, alternatively the maintenance dose may be O.Olmg/kg to 2mg/kg; alternatively the maintenance dose may be O. lmg/kg to lmg/kg; alternatively the maintenance dose may be O. lmg/kg to 0.8mg/kg; alternatively the maintenance dose may be O. lmg/kg to 0.6mg/kg; alternatively the maintenance dose may be O.lmg/kg to 0.4mg/kg; alternatively the maintenance dose may be O.lmg/kg to 0.2mg/kg.
The loading dose may be O.OOOlmg/kg (mass of drug compared to mass of patient) to 20mg/kg, and the maintenance dose may be between 0.0001 mg/kg to 20mg/kg; alternatively the loading dose may be 0.001 mg/kg to 10 mg/kg, alternatively the loading dose may be 0.01 mg/kg to 2 mg/kg; alternatively the loading dose may be O.lmg/kg to lmg/kg; alternatively the loading dose may be O.lmg/kg to lmg/kg; alternatively the loading dose may be 0.2mg/kg to 0.8mg/kg; alternatively the loading dose may be 0.3mg/kg to 0.6mg/kg; alternatively the loading dose may be 0.4mg/kg to 0.6mg/kg.The agent will generally be administered in conjunction with a pharmaceutically acceptable carrier. The term "pharmaceutically acceptable carrier", as used herein, includes genes, polypeptides, antibodies, liposomes, polysaccharides, polylactic acids, polyglycolic acids and inactive virus particles or indeed any other agent provided that the carrier does not itself induce toxicity effects or cause the production of antibodies that are harmful to the individual receiving the pharmaceutical composition. Pharmaceutically acceptable carriers may additionally contain liquids such as water, saline, glycerol, ethanol or auxiliary substances such as wetting or emulsifying agents, pH buffering substances and the like.
The pharmaceutical carrier employed will thus vary depending on the route of administration. Carriers may enable the pharmaceutical compositions to be formulated into tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions to aid intake by the patient. A thorough discussion of pharmaceutically acceptable carriers is available in id="p-31" id="p-31"
id="p-31"
[31].
The agent may be delivered by any known route of administration. The agent may be delivered locally or systemically. The agent may be delivered by a parenteral route (e.g. by injection, either subcutaneously, intraperitoneally, intravenously or intramuscularly or delivered to the interstitial space of a tissue). The compositions can also be administered into a lesion. Other modes of administration include oral and pulmonary administration, suppositories, and transdermal or transcutaneous applications, needles, and hyposprays.
Preferably the agent is delivered via subcutaneous injection. In some embodiments this is via once daily subcutaneous injection, for example at an initial loading dose of between O.OOOlmg/kg (mass of drug compared to mass of patient) to 20mg/kg, followed by once daily maintenance doses of between O.OOOlmg/kg to 20mg/kg, or other doses disclosed elsewhere herein. Alternatively the agent may be delivered via subcutaneous injection every other day.
In a preferred embodiment the agent is delivered via once daily subcutaneous injection at an initial loading dose of 0.4mg/kg-0.6mg/kg (for example O.S7mg/kg) followed by once daily maintenance doses of O.lmg/kg-0.2mg/kg (for example 0.14mg/kg).
The agent may be administered alone or as part of a treatment regimen also involving the S administration of other drugs currently used in the treatment of patients with a complement-mediated disease or disorder.
The agent may be administered simultaneously, sequentially or separately with the other drug(s). For example, the agent may be administered before or after administration of the other drug(s). In particular, the agent may be administered after a pervious drug has failed to threat the complement mediated disease or disorder. In a specific embodiment, the agent may be administered after an anti-CS monoclonal antibody.
In particular embodiments: (i) the complement-mediated disease is paroxysmal nocturnal haemoglobinuria (PNH); (ii) the complement CS polymorphism is at residue Arg88S; lS (iii) the agent for treatment is Coversin protein or fragments or homologues of the Coversin protein providing that such fragments retain the ability to bind wild-type CS and/or CS from subjects with a CS polymorphism; (iv) the agent is delivered subcutaneously.
In certain embodiments the subcutaneous injection is once daily at an initial loading dose of 0.4mg/kg-0.6mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of O. lmg/kg-0.2mg/kg; more preferably at an initial loading dose of O.S7mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of0.14mg/kg.
In particular embodiments: 2S (i) the complement-mediated disease is graft versus host disease (GvHD; (ii) the complement CS polymorphism is at residue Arg88S; (iii) the agent for treatment is Coversin protein or fragments or homologues of the Coversin protein providing that such fragments retain the ability to bind wild-type CS and/or CS from subjects with a CS polymorphism; (iv) the agent is delivered subcutaneously.
In certain embodiments the subcutaneous injection is once daily at an initial loading dose of 0.4mg/kg-0.6mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of O. lmg/kg-0.2mg/kg; more preferably at an initial loading dose of S O.S7mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of0.14mg/kg.
In particular embodiments: (i) the complement-mediated disease is thrombotic thrombocytopaenicpurpura (TTP); (ii) the complement CS polymorphism is at residue Arg88S; (iii) the agent for treatment is Coversin protein or fragments or homologues of the Coversin protein providing that such fragments retain the ability to bind wild-type CS and/or CS from subjects with a CS polymorphism; (iv) the agent is delivered subcutaneously.
In certain embodiments the subcutaneous injection is once daily at an initial loading dose IS of 0.4mg/kg-0.6mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of O. lmg/kg-0.2mg/kg; more preferably at an initial loading dose of O.S7mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of0.14mg/kg.
In particular embodiments: (i) the complement-mediated disease is atypical haemolytic uremeic syndrome (aHUS); (ii) the complement CS polymorphism is at residue Arg88S; (iii) the agent for treatment is Coversin protein or fragments or homologues of the Coversin protein providing that such fragments retain the ability to bind wild-type CS and/or CS from subjects with a CS polymorphism; 2S (iv) the agent is delivered subcutaneously.
In certain embodiments the subcutaneous injection is once daily at an initial loading dose of 0.4mg/kg-0.6mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of O. lmg/kg-0.2mg/kg; more preferably at an initial loading dose of 0.57mg/kg (mass of drug compared to mass of patient), followed by once daily maintenance doses of0.14mg/kg.
Various aspects and embodiments of the present invention will now be described in more detail by way of example. It will be appreciated that modification of detail may be made without departing from the scope of the invention.
BRIEF DESCRIPTION OF FIGURES: Figure 1: Schematic diagram of classical and alternative pathways of complement activation. Enzymatic components, dark grey. Anaphylatoxins enclosed in starbursts.
Figure 2: Primary sequence of Coversin. Signal sequence underlined. Cysteine residues in bold type. Nucleotide and amino acid number indicated at right.
Figure 3: Peak and trough platelet counts from patient treated in Example 2 Figure 4: In vitro testing of serum from patient in Example 4 by CH50 assay after spiking with variable doses of Coversin and eculizumab (expressed as a percentage of control).
Shows percentage complement activity in serum from patient in Example 4 compared to control serum in presence of Eculizumab or Coversin. KEY: Ecu, spiked with Eculizumab; Cov, spiked with Coversin. NC3, normal control serum; R2, patient serum.
Figure 5: In vitro testing of serum from patient in Example 4 by CH50 assay after spiking with variable doses of Coversin and eculizumab. Shows complement activity in CH50 Eq/ml units in serum from patient in Example 4 compared to control serum in presence of Eculizumab or Coversin. KEY: Ecu, spiked with Eculizumab; Cov, spiked with Coversin.
NC3, normal control serum; R2, patient serum.
Figure 6: In vitro testing of serum from patient in Example 3 by CH50 assay after spiking with variable doses of Coversin and eculizumab (expressed as a percentage of control) Shows percentage complement activity from patient in Example 3 compared to control serum in presence of Eculizumab or Coversin. KEY: Ecu, spiked with Eculizumab; Cov, spiked with Coversin. NC, normal control serum; BJI, replicate 1 using patient serum; BJ2 replicate 2 using patient serum.
Figure 7: In vitro testing of serum from patient in Example 3 by CH50 assay after spiking with variable doses of Coversin and eculizumab. Shows complement activity in CH50 Eq/ml units from patient in Example 3 in presence ofEculizumab or Coversin. KEY: Ecu, spiked with Eculizumab; Cov, spiked with Coversin. BJI and BJ2 referred to as Pat la and Pat lb.
Figure 8: Molecular model showing the position of the eculizumab epitope and the likely binding site of Coversin EXAMPLES Example 1 - in vitro inhibition of CS activity Terminal complement activity was measured in serum from a 4 year old, male, Caucasian patient found to have a rare genetic polymorphism in the gene encoding complement CS (c.2654G>A (p.Arg885His)) by Quidel CH haemolysis assay.
The Quidel Microvue CH50 Eq enzyme immunoassay (cat #A018) was used is for in vitro measurement of total classical pathway activity m human serum. http://www.quidel.com/ sites/ quidel.com/ files/product/ documents/ aO 18 _ microvue _ ch5 0 _ eq _english_l.pdf The kit provides a direct measure of the terminal complement complex (TCC) formation under standard conditions. Measurement of CH50 with the kit has 3 steps: 1. Activation of the classical complement pathway in undiluted serum resulting in formation ofTCC. 2. Dilution of serum and addition to microassay wells coated with an antibody that captures TCC. 3. Quantification of captured TCC with and anti-TCC horse radish peroxidase (HRP) conjugated antibody.
Colour intensity on addition of substrate is proportional to the concentration of TCC present in each reaction. Using the kit standard curve (determined during each assay) assay results are expressed in CH50 unit equivalents per millilitre (CH50 U Eq/ml).
The linear range for the kit is 30-310 U Eq/ml.
According to the manufacturers the cut off for normality determined from 234 individual human samples is 70 CH50 U Eq/ml.
Following treatments with eculizumab, the patient retained 70% complement activity as compared to complement activity in the serum of a normal control with wild-type CS.
Spiking the serum taken after administration of eculizumab with 30, 60 and 120µg/ml Coversin resulted in undetectable levels of complement activity.
S Thus, in a non-responder to eculizumab, Coversin retained normal effectiveness.
Example 2 - case study A 4 year old, male, Caucasian patient, weighing 13.6 kg, received a primary diagnosis of chronic granulomatous disease and underwent haematopoietic stem cell transplantation in October 2013. Subsequently the patient developed major gastrointestinal bleeding due to thrombocytopenia and is now receiving daily platelet transfusions. The diagnosis is either graft versus host disease (GvHD) or thrombotic thrombocytopaenicpurpura (TTP).
Treatment with eculizumab, infliximab and rituximab have been unsuccessful.
The patient has been found to have a rare genetic polymorphism in the gene encoding complement CS (c.26S4G>A (p.Arg88SHis)), previously only described in people of IS Japanese or Han Chinese origin.
In vitro assays of serum complement activity as described above showed that complement activity result was ~70% haemolytic activity compared to normal control after treatment with eculizumab. In contrast, spiking the serum with Coversin at 30, 60 and 120µg/ml reduced haemolytic activity to undetectable levels.
Following identification of susceptibility to inhibition of the complement pathways by Coversin, the following treatment was begun: Coversin, by subcutaneous injection according to the following schedule: Initial Loading Dose: O.S7mg/kg = 7.8mg (0.7ml) Maintenance Dose: 0.14mg/kg = 1.9mg (0.2ml) every 24 hours thereafter 2S Serum will be taken daily for complement activity and dose and/or frequency will be adjusted in order to maintain terminal complement activity at 10% or less compared to normal control serum.
The following outcomes will also be monitored: a) Change in trough platelet counts b) Change in serum LDH c) Terminal complement activity measured by Quidel CH haemolysis assay Example 3 - results of case study The patient of Example 2 was treated with Coversin for about 6 weeks. On the first day of S treatment he received a dose calculated to ablate circulating CS (O.S7mg/kg) and thereafter SO% of this dose until the end of the second week. From then the patient received the same dose every other day for two weeks and then half that dose for a further two weeks. It should be noted that the dose from the third week onwards was likely to have been inadequate to fully control terminal complement activity.
Clinically the patient stabilised during the period that he received the full dose. The main consequence of his illness, which was presumed to be a thrombotic thromocytopaenic purpura (TTP), was severely reduced platelet count for which he had been receiving two units of platelets every day for several months. After 7 days of Coversin treatment the trough platelet counts (approximately 12 hours post platelet transfusion) began to rise lS reaching 98,000 by Day 14, the highest value that had been recorded throughout his illness.
His requirement for platelet transfusion was reduced to one unit per day at that point (see Figure 3).
The dose was reduced at the start of the third week and the trough platelet count fell to below S0,000 and did not recover for the remainder of his illness. The rise in trough platelet count and the reduced need for platelet transfusion was considered by the medical staff as a clear indication of a positive response to Coversin. The deterioration after the dose was reduced seems to confirm this.
The final dose of Coversin was given after 6 weeks and the patient rapidly deteriorated and died from perforation of the jejunum after a further 2 weeks.
Example 4 - case study A male patient aged in his mid-forties was diagnosed with PNH and he has been treated with eculizumab for about a year with an inadequate clinical response. Genetic analysis has confirmed a heterozygous CS polymorphism at position c.26S4 but it is not known what amino acid shift this gives rise to although it is known that it is not pArg88SHis.
Example 5 - Terminal complement activity in serum from patients Reagents & Samples Sample Preparation: Serum was prepared by collecting blood into plain glass or SST Vacutainer tubes (or equivalent) and allowing it to clot for 1 hour, before centrifugation at 1500g for 10 minutes. The serum was separated immediately (avoiding contamination with any blood cells) and stored in screw cap cryotubes (approximately 0.5ml aliquots) at - 70°C.
Coversin: Frozen 10.9mg/ml solution at -70°C. Dilute lOuL in 90uL normal control or patient serum to give a final concentration of 1.09mg/ml. Dilute lOuL in 90uL autologous serum to give a final concentration of 109ug/ml. Double dilute in autologous serum to achieve a final concentration range of: 0.4 - 54.5ug/ml.
Eculizumab: Frozen solution of lOmg/ml. Dilute lOuL in 90uL normal control or patient serum to give a final concentration of lmg/ml. Dilute lOuL in 90uL autologous serum to give a final concentration of 1 OOug/ml. Double dilute in autologous serum to achieve a final concentration range of: 0.4 - 50ug/ml.
Buffer: Phosphate Buffered Saline (O.OlM phosphate buffer, 0.0027M potassium chloride, 0.137M sodium chloride, pH7.4).
Methods Coversin, Eculizumab, or buffer (control), are spiked into serum according to the procedure above to achieve a range of final concentrations. These are then assayed for CH50 Equivalent activity using the Quidel CH50 kit, using duplicate wells.
Results Calculate CH50 values from the calibration curve provided with the kit. Plot the results as raw CH50 values against CS inhibitor concentration.
Calculate the CHSO result at each CS inhibitor concentration as a percentage of the CHSO concentration of the relevant buffer control. Plot the percentage CHSO results against inhibitor concentration.
Repeat the experiment on separate days to obtain 3 measurements in each patient and in a single normal control. This provides an estimate of between experiment variability.
Repeat the experiment on separate days in single experiments on 6 different normal controls. This provides an estimate of between subject responsiveness (and avoids the risk of using a single subject who may have an unknown CS mutation or polymorphism).
The highest dose of each drug to whole serum was added and then two-fold serial dilutions were made in whole serum. One replicate was used for each drug dose.
The highest dose of Eculizumab was SOµg/ml, then 2S, 12.S, 6.3, 3.2, 1.6, 0.8, 0.4 and lS Oµg/ml. The highest dose of Coversin was S4.Sµg/ml, then 27.3, 13.1, 6.6. 3.3, 1.7, 0.9 and Oµg/ml.
After serial dilution the serum was activated and assayed m accordance with the instructions for the Quidel CHSO kit.
CHSO U Eq/ml were calculated in comparison with the kit standards and plotted against drug concentration for each of the three serum samples and two drug treatments. They were also plotted as a percentage of the CHSO value of the relevant buffer only control.
Normal human serum and serum from the patients in the case studies were tested for terminal complement activity in the presence of Eculizumab and Coversin as described 2S above.
As shown in Figures 6 and 7, in the absence of either drug the baseline CHSO values of the normal human serum (average 78.1 CHSO U Eq/ml) and the two patient serum samples from the patient described in the case study of examples 2 and3 (average 82.4 and 60.6 CH50 U Eq/ml) were within (normal control and BJ 2) or slightly below (BJ 1) the normal human range of>70 CH50 U Eq/ml.
Coversin inhibited both normal human serum and serum from the patient with the p.Arg885His polymorphism equally well. Less than 5% of baseline CH50 (U Eq/ml) was seen at Coversin concentrations of approximately 15ug/ml.
Eculizumab inhibited normal human serum at the expected dose, with less than 5% of baseline CH50 (U Eq/ml) seen at concentrations of approximately 45ug/ml. At doses above 25ug/ml eculizumab inhibited complement activity measured using the Quidel CH50 kit similarly in normal human serum and serum from the patient with the p.Arg885His polymorphism. However, it did not fully inhibit serum from the patient, with approximately 20% of baseline CH50 remaining at the highest dose of eculizumab tested (60ug/ml).
Serum from the patient described in Example 4 was also tested in parallel with normal human serum. As shown in Figures 4 and 5, in absence of either drug the baseline CH50 values of the normal human serum and the serum from the patient serum samples were within the normal human range of>70 CH50 U Eq/ml.
Coversin inhibited both normal human serum and serum from the patient with an amino acid substitution at Arg885 equally well. Less than 5% of baseline CH50 (U Eq/ml) was seen at Coversin concentrations of approximately 15ug/ml.
Eculizumab inhibited normal human serum at the expected dose, with less than 5% of baseline CH50 (U Eq/ml) being achieved. Akin to the patient serum from Example 2, at doses above 25ug/ml eculizumab inhibited complement activity similarly in normal human serum and serum from the Example 4 patient, but it did not completely inhibit serum from the Example 4 patient, with approximately 10% of baseline CH50 remaining at the highest dose of eculizumab tested (50ug/ml).
Eculizumab does not completely inhibit complement activity in serum from both patients (Example 2 and Example 4) who received no benefit from therapeutic treatment with eculizumab. This supports the hypothesis that complement inhibition in PNH treatment S needs to be higher than this to see therapeutic benefit.
Using recombinant expression Nishimura et al. (2014) showed that the CS p.Arg88SHis polymorphism seen in the Example 2 patient completely ablates eculizumab binding to CS.
The partial inhibition of the Example 2 patient's complement serum by eculizumab shown in the current study (Figures 6 and 7) is understandable as the Example 2 patient and all other individuals with the polymorphism identified to date are heterozygotes with a normal copy of CS and a copy of p.Arg88SHis CS. If both copies are fully expressed, eculizumab will fully inhibit SO% of the CS protein present in these individuals. The fact that only 20% residual CHSO activity was seen may reflect the fact that the Example 2 patient was lS receiving fresh blood products every day which likely increased the ratio of normal CS to p.Arg88SHis CS, thus reducing the relative amount of CS p.Arg88SHis not inhibited by eculizumab.
Eculizumab appears to inhibit the Example 4 patient's serum to a greater extent than the Example 2 patient's serum, though some residual complement activity remains at even the highest dose of eculizumab. A possible explanation is that the amino acid change at Arg88S is a conservative one that has a less profound effect on eculizumab binding than p.Arg88SHis. 2S By contrast, Coversin is an equally effective inhibitor of normal human serum, and the serum from the two patients which are not fully inhibited by eculizumab. Complete inhibition by Coversin is understandable as it likely binds to a different site on CS than eculizumab. Furthermore Coversin has been shown to be an equally effective inhibitor of CS in a wide range of mammalian species including man, cynomologus monkey, pig, rat, mouse, rabbit and guinea pig. This indicates that binding of Coversin to CS is far more tolerant of differences in the amino acid sequence of CS than eculizumab which is only able to inhibit human CS. Coversin should be considered for treatment of patients that would benefit from CS inhibition but who gain little or no therapeutic treatment from administration of eculizumab due to polymorphisms in CS that prevent or reduce the affinity of the binding interaction between eculizumab and CS.
REFERENCES: Pfarr N, et al. Jimmunol. 174(7):4172-7. (2005) Erratum in: Jimmunol.;182(8):5152 Delgado-Cervifio E, Fontan G, L6pez-Trascasa M. Mol Immunol. 42(1):105-11. (2005) Halangk J, et al., J Hepatol. 49(3):339-45 (2008) Nishimura, Jet a;., New Engl J. Med., 30;7: 632-639 (2014) Wang X, Fleischer DT, Whitehead WT, Haviland DL, Rosenfeld SI, Leddy JP, Snyderman R, Wetsel RA. J Immunol. 154(10):5464-71. (1995) Jakowski et al., Clin Diagn Lab Immunol. 6(1): 137-139 (1999) Freslund et al., Nature Immunology 9, 753 - 760 (2008) Zuber et al. Nature Reviews Nephrology 8, 643-657 Hobart, M. J., et al., Annals of Human Genetics 45.1 (1981): 1-4.
Jakowski et al., Clin Diagn Lab Immunol. 6(1): 137-139 (1999) [ 11] Sahu et al., Immunopharmacology, 49: 133-148 (2000).
Ricklin D & Lambris J, Nature Biotechnology, 25: 1265-1275 (2007) Guo, R.F. and P.A. Ward, Annu Rev Immunol, 2005, 23: p. 821-52 Neumann, E., et al., Arthritis Rheum, 2002. 46(4): p. 934-45 Williams, AS., et al., Arthritis Rheum, 2004, 50(9): p. 3035-44 Quigg, R.J., Curr Dir Autoimmun, 2004. 7: p. 165-80 Papagianni, A.A., et al., Nephrol Dial Transplant, 2002, 17(1): p. 57-63 He, C., et al., J Immunol, 2005. 174(9): p. 5750-7 Mead, R.J., et al., J Immunol, 2002. 168(1): p. 458-65 Nakashima, S., et al., J Immunol, 2002. 169(8): p. 4620-7 Terpe K, Appl Microbiol Biotechnol, 60: 523-33, 2003 Sambrook et al (2000) Fernandez & Hoeffler (1998) Ausubel et al. (1991) Remington's Pharmaceutical Sciences; Mack Pub. Co., N.J. 1991
Claims (12)
1. The use of an agent for the manufacture of a medicament for treating or preventing a complement-mediated disease and/or disorder in a subject known to have a complement C5 polymorphism, wherein the agent inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway, and wherein the agent is: (a) a protein comprising or consisting of amino acids 19 to 168 of the amino acid sequence according to SEQ ID NO: 2 or is a functional equivalent of this protein; (b) a protein comprising or consisting of amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2 or is a functional equivalent of this protein; or (c) a nucleic acid molecule encoding a protein as recited in (a) or (b); and wherein the complement C5 polymorphism is at the Arg885 residue of wildtype C5 and decreases the effectiveness of eculizumab; wherein the functional equivalent of: (a) a protein comprising or consisting of amino acids 19 to 168 of the amino acid sequence according to SEQ ID NO: 2; or (b) a protein comprising or consisting of amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2; is i. a homologue comprising or consisting of a sequence with at least 80% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2; or ii. a fragment of a protein, wherein the protein has the amino acid sequence according to SEQ ID NO: 2, or a fragment of a homologue, wherein the homologue has at least 80% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: wherein said functional equivalent retains the ability to bind complement C5 and to prevent the cleavage of complement C5 by C5 convertase into complement C5a and complement C5b-9. 1004805438
2. The use according to claim 1, wherein the subject known to have a complement C5 polymorphism has been identified by: (a) an unexpectedly poor clinical response to an agent that inhibits one of the complement pathways; and/or (b) testing the ability of an agent that inhibits one of the complement pathways to inhibit complement activation in the subject; and/or (c) molecular genetic analysis.
3. The use according to claim 1 or claim 2, wherein the complement C5 polymorphism is Arg885Cys.
4. The use according to claim 1 or claim 2, wherein the complement C5 polymorphism is Arg885His.
5. The use according to any one of claims 1 to 4, wherein the complement C5 polymorphism has been identified or confirmed by sequencing the gene encoding C5 or other molecular genetic analysis.
6. The use according to any one of claims 1 to 5, wherein the functional equivalent of: (a) a protein comprising or consisting of amino acids 19 to 168 of the amino acid sequence according to SEQ ID NO: 2; or (b) a protein comprising or consisting of amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2; is (c) a homologue comprising a sequence with at least 90% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2; or (d) a fragment of a protein, wherein the protein has the amino acid sequence of SEQ ID NO: 2, or a fragment of a homologue, wherein the homologue has at least 90% identity 1004805438 to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2, wherein said functional equivalent retains the ability to bind complement C5 and to prevent the cleavage of complement C5 by C5 convertase into complement C5a and complement C5b-9.
7. The use according to claim 6 wherein the functional equivalent of: (a) a protein comprising or consisting of amino acids 19 to 168 of the amino acid sequence according to SEQ ID NO: 2; or (b) a protein comprising or consisting of amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2; (c) a homologue comprising a sequence with at least 95% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2; or (d) a fragment of a protein, wherein the protein has the amino acid sequence of SEQ ID NO: 2, or a fragment of a homologue, wherein the homologue has at least 95% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2, wherein said functional equivalent retains the ability to bind complement C5 and to prevent the cleavage of complement C5 by C5 convertase into complement C5a and complement C5b-9.
8. The use according to claim 6, wherein the functional equivalent of: (a) a protein comprising or consisting of amino acids 19 to 168 of the amino acid sequence according to SEQ ID NO: 2; or (b) a protein comprising or consisting of amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2; 1004805438 (c) a homologue comprising a sequence with at least 98% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2; or (d) a fragment of a protein, wherein the protein has the amino acid sequence of SEQ ID NO: 2, or a fragment of a homologue, wherein the homologue has at least 98% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2, wherein said functional equivalent retains the ability to bind complement C5 and to prevent the cleavage of complement C5 by C5 convertase into complement C5a and complement C5b-9.
9. The use according to claim 6, wherein the functional equivalent of: (a) a protein comprising or consisting of amino acids 19 to 168 of the amino acid sequence according to SEQ ID NO: 2; or (b) a protein comprising or consisting of amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2; (c) a homologue comprising a sequence with at least 99% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2; or (d) a fragment of a protein, wherein the protein has the amino acid sequence of SEQ ID NO: 2, or a fragment of a homologue, wherein the homologue has at least 99% identity to the sequence of amino acids 19 to 168 or amino acids 1 to 168 of the amino acid sequence SEQ ID NO: 2, wherein said functional equivalent retains the ability to bind complement C5 and to prevent the cleavage of complement C5 by C5 convertase into complement C5a and complement C5b-9.
10. The use according to any one of claims 1 to 9, wherein the agent is: 1004805438 (a) a protein consisting of amino acids 19 to 168 of the amino acid sequence according to SEQ ID NO: 2; or (b) a protein consisting of amino acids 1 to 168 of the amino acid sequence according to SEQ ID NO: 2; or (iii) a nucleic acid molecule encoding the protein as recited in (i) or (ii).
11. The use according to any one of claims 1 to 10, wherein the complement-mediated disease and/or disorder is selected from: acute pancreatitis, age related macular degeneration (AMD), atypical haemolytic uremeic syndrome (aHUS), Alzheimer’s disease, Huntingdon’s disease, Parkinson’s disease, allergic encephalomyelitis, allotransplatation, asthma, adult respiratory distress syndrome, influenza, burn injuries, Crohn’s disease, glomerulonephritis, haemolytic anaemia, haemodialysis, hereditary angioedema, ischaemia reperfusion injuries, multiple system organ failure, multiple sclerosis, myasthenia gravis, myocardial infarction, paroxysmal nocturnal haemoglobinuria (PNH), psoriasis, rheumatoid arthritis, septic shock, systemic lupus erythematosus, stroke, thrombotic thrombocytopaenicpurpura (TTP), traumatic brain injury, vascular leak syndrome, and transplantation rejection and graft versus host disease (GvHD), peripheral nerve disorders and respiratory disorders.
12. The use according to claim 11, wherein the complement-mediated disease and/or disorder is selected from: paroxysmal nocturnal haemoglobinuria, graft versus host disease, thrombotic thrombocytopaenic purpura and atypical haemolytic uremic syndrome. 1004805438 Fl . 1 Classical Alternative Immune complexes Complex carbohydrates E C3 convertase E C5 convertase SUBSTITUTE SHEET (RULE 26) ..... ic::t'
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB1410116.6A GB201410116D0 (en) | 2014-06-06 | 2014-06-06 | Method of treatment |
PCT/EP2015/062742 WO2015185760A1 (en) | 2014-06-06 | 2015-06-08 | Ornithodoros moubata complement inhibitor for use in the treatment of complement-mediated diseases in patients with c5 polymorphism |
Publications (2)
Publication Number | Publication Date |
---|---|
NZ727053A NZ727053A (en) | 2023-10-27 |
NZ727053B2 true NZ727053B2 (en) | 2024-01-30 |
Family
ID=
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20210283221A1 (en) | Ornithodoros Moubata Complement Inhibitor for Use in the treatment of Complement-Mediated Diseases in Patients with C5 Polymorphism | |
US20190117730A1 (en) | Method of Treating Peripheral Nerve Disorders | |
EP3302519B1 (en) | Ornithodoros moubata complement inhibitor for use in the treatment of acute graft versus host disease | |
JP5784078B2 (en) | Respiratory disease treatment methods | |
US20240009270A1 (en) | Coversin for the treatment of autoimmune blistering diseases | |
JP2009508819A (en) | Treatment for myasthenia gravis | |
WO2017140903A1 (en) | Method of treatment of complement mediated neurodevelopmental disorder secondary to zika virus infection | |
NZ727053B2 (en) | Ornithodoros moubata complement inhibitor for use in the treatment of complement-mediated diseases in patients with c5 polymorphism | |
DK2061501T3 (en) | METHOD OF TREATING RESPIRATORY DISORDERS |