NZ710003B2 - Positive allosteric modulators of nicotinic acetylcholine receptor - Google Patents

Positive allosteric modulators of nicotinic acetylcholine receptor Download PDF

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Publication number
NZ710003B2
NZ710003B2 NZ710003A NZ71000312A NZ710003B2 NZ 710003 B2 NZ710003 B2 NZ 710003B2 NZ 710003 A NZ710003 A NZ 710003A NZ 71000312 A NZ71000312 A NZ 71000312A NZ 710003 B2 NZ710003 B2 NZ 710003B2
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New Zealand
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compound
phenyl
amide
ethyl
acid
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NZ710003A
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NZ710003A (en
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J Rgen Eskildsen
Ask Puschl
Anette Graven Sams
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H Lundbeck A/S
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Abstract

Disclosed herein are compounds of formula (I) where the substituents are as defined herein, compositions comprising said compounds, and to methods of treating diseases comprising administration of said compounds. The compounds referred to are positive allosteric modulators (PAMs) of the nicotinic acetylcholine ?7 receptor. etylcholine ?7 receptor.

Description

[Annotation] sak TITLE POSITIVE ALLOSTERIC MODULATORS OF NICOTINIC CHOLINE RECEPTOR FIELD OF THE ION The present invention relates to compounds useful in therapy, to compositions com- prising said compounds, and to methods of treating diseases sing administration of said compounds. The compounds referred to are positive a||osteric modulators (PAMs) of the nicotinic acetylcholine o7 or.
BACKGROUND OF THE INVENTION Nicotinic acetylcholine receptors (nAChRs) belong to the super family of ligand gated ionic ls, and gate the flow of cations ing calcium. The nAChRs are endogenously activated by acetylcholine (ACh) and can be divided into nicotinic receptors of the neuromus- cularjunction and neuronal nicotinic receptors (NNRs). The NNRs are widely expressed throughout the central nervous system (CNS) and the peripheral nervous system (PNS). The NNRs have been suggested to play an important role in CNS function by modulating the re- lease of many neurotransmitters, for example, ACh, norepinephrine, dopamine, serotonin, and GABA, among others, resulting in a wide range of physiological effects.
Seventeen subunits of nAChRs have been reported to date, which are identified as 012-0110, , v, 6 and a. From these subunits, nine ts, (12 through (17 and [32 through [34, prominently exist in the mammalian brain. Many functionally distinct nAChR xes exist, for example five (17 subunits can form a receptor as a ric functional pentamer or combinations of different subunits can form heteromeric receptors such as (1482 and (1384 receptors (Gotti, C. et al., Prog. iol., 2004, 74: 363-396; Gotti, C. et al., Biochemical Pharmacology, 2009, 78: 703—71 1) The homomeric d7 receptor is one of the most abundant NNRs, along with (1482 re- ceptors, in the brain, wherein it is heavily expressed in the hippocampus, cortex, ic nu- clei, ventral tegmental area and substantia nigra (Broad, L. M. et al., Drugs of the Future, 2007, 32(2): 161-170, Poorthuis RB, Biochem Pharmacol. 2009, 1;78(7):668—76).
The role of (17 NNR in neuronal signalling has been actively igated. The (17 NNRs have been demonstrated to regulate interneuron excitability and modulate the e of excitatory as well as inhibitory neurotransmitters. In addition, (17 NNRs have been reported to be involved in neuroprotective effects in experimental models of cellular damage (Shimo- hama, 8., Biol Pharm Bull. 2009, 32(3):332-6).
[Annotation] sak Studies have shown that (17 subunits, when expressed recombinant in-vitro, activate and de- sensitize rapidly, and exhibit relatively higher calcium permeability compared to other NNR combinations (Papke, R.L. et al., J col Exp Ther. 2009, 329(2):791-807).
The NNRs, in l, are involved in various cognitive functions, such as ng, memory and ion, and therefore in CNS ers, i.e., Alzheimer's disease (AD), Parkinson's disease (PD), attention t hyperactivity disorder (ADHD), Tourette's syn- drome, schizophrenia, bipolar er, pain and tobacco dependence (Keller, J. J. et al., Be- hav. Brain Res. 2005, 162: ; Haydar, S.N. et al., Curr Top Med Chem. 2010;10(2):144- 52).
The G7 NNRs in ular, have also been linked to cognitive disorders including, for example, ADHD, autism um disorders, AD, mild cognitive impairment (MCI), age asso- ciated memory impairment (AAMI) senile dementia, frontotemporal lobar degeneration, HIV associated dementia (HAD), HIV associated cognitive impairment (HIV-Cl), Pick's disease, ia associated with Lewy , cognitive impairment associated with Multiple Sclero- sis, Vascular Dementia, cognitive impairment in Epilepsy, cognitive impairment associated with fragile X, cognitive impairment associated with Friedreich’s Ataxia, and dementia associ- ated with Down's syndrome, as well as cognitive impairment associated with schizophrenia.
In addition, s have been shown to be involved in the neuroprotective effects of nico- tine both in vitro (Jonnala, R. B. et al., J. Neurosci. Res., 2001, 66: 565- 572) and in vivo (Shimohama, 8., Brain Res., 1998, 779: 359-363) as well as in pain signalling. More particularly , neurodegeneration underlies l progressive CNS disorders, including, but not lim- ited to, AD, PD, amyotrophic lateral sclerosis, Huntington's disease, dementia with Lewy bod- ies, as well as diminished CNS function resulting from traumatic brain injury. For example, the impaired function of (17 NNRs by beta-amyloid peptides linked to AD has been implicated as a key factor in development of the cognitive deficits associated with the disease (Liu, Q.-S., et al., PNAS, 2001,98: 4734-4739). Thus, modulating the ty of (17 NNRs demonstrates promising potential to prevent or treat a variety of diseases indicated above, such as AD, other ias, other neurodegenerative diseases, schizophrenia and neurodegeneration, with an underlying ogy that involves cognitive function including, for example, aspects of learning, memory, and attention (Thomsen, M.S. et al., Curr Pharm Des. 2010 Jan;16(3):323-43; Olincy, A. et al., Arch Gen Psychiatry. 2006, 63(6):630-8; Deutsch, S.|., Clin Neuropharmacol. 2010, 33(3):114-20; Feuerbach, D., Neuropharmacology. 2009, 56(1): The NNR ligands, including (17 ligands, have also been implicated in weight control, diabetis inflammation, obsessive-compulsive disorder (OCD), angiogenesis and as potential analgesics (Marrero, M.B. et al., J. Pharmacol. Exp. Ther. 2010, 332(1):173-80; Vincler, M., [Annotation] sak Exp. nvest. Drugs, 2005, 14 (10): 1191-1 198; Rosas-Ballina, M., J. Intern Med. 2009 265(6):663-79; Arias, H.R., Int. J. Biochem. Cell Biol. 2009, 41(7):1441-51; Tizabi, Y., Biol Psychiatry. 2002, 51 (2):164-71).
Nicotine is known to enhance ion and cognitive performance, reduced anxiety, enhanced sensory gating, and analgesia and neuroprotective effects when administered.
Such effects are mediated by the non-selective effect of nicotine at multiple nicotinic receptor subtypes. However, nicotine also exerts adverse events, such as cardiovascular and gastro- intestinal problems (Karaconji, |.B. et al., Arh Hig Rada Toksikol. 2005, 363-71). Con- sequently, there is a need to identify subtype-selective compounds that retain the beneficial effects of nicotine, or an NNR ligand, while eliminating or decreasing adverse effects.
Examples of ed NNR ligands are or? NNR agonists, such as DMXB-A, SSR180711 and ABT-107, which have shown some beneficial effects on cognitive processing both in rodents and humans (H312: 1213-22; , A. et al., Arch Gen Psychiatry. 2006 63(6):630-8; Pichat, P., et al., Neuropsychopharmaco/ogy. 2007 32(1):17-34; Bitner, R.S., J Pharmacol Exp Ther. 2010 1;334(3):875-86). In addition, modulation of or? NNRs have been ed to improve ve ms in patients with phrenia (Freedman, R. et al., Am J Psychiatry. 2008 :1040-7).
Despite the beneficial effects of NNR ligands, it remains uncertain whether chronic treatment with agonists affecting NNRs may provide suboptimal benefit due to sustained acti- vation and desensitization of the NNRs, in particular the o7 NNR subtype. In contrast to ago- nists, administering a positive eric modulator (PAM) can reinforce endogenous choliner- gic ission without directly stimulating the target receptor. Nicotinic PAMs can selec- tively modulate the activity of ACh at NNRs, preserving the activation and deactivation kinet- ics of the receptor. Accordingly, o7 NNR-selective PAMs have emerged (Faghih, R., Recent Pat CNS Drug Discov. 2007, 2(2):99—106). uently, it would be beneficial to increase or? NNR on by enhancing the ef- fect of the nous neurotransmitter acetvlcholine via PAMs. This could reinforce the en- dogenous cholinergic neurotransmission without directly activating o7 NNRs, like agonists.
Indeed, PAMs for ing channel activity have been proven clinically successful for GABAa receptors where benzodiazepines and barbiturates, behave as PAMs acting at dis- tinct sites (Hevers, W. et al., Mol. Neurobiol., 1998, 18: 35-86).
To date, only a few NNR PAMs are known, such as 5-hydroxyindole (5-Hl), ivermec- tin, galantamine, and SLURP-1, a peptide derived from acetylcholinesterase (AChE). Gen- , a kinase inhibitor was also reported to increase or? responses. PNU-120596, a urea derivative, was reported to increase the potency of ACh as well as improve auditory gating ts induced by amphetamine in rats. Also, NS1738, JNJ-1930942 and compound 6 have ation] sak been reported to potentiate the response of ACh and exert beneficial effect in experimental models of sensory and cognitive processing in rodents. Other NNR PAMs include derivatives of quinuclidine, indole, benzopyrazole, le, and benzoisothiazoles (Hurst, R. S. et al., J. ci. 2005, 25: 4396-4405; Faghih, R., Recent Pat CNS Drug Discov. 2007, 2(2):99—106; Timmermann, D.B., J. Pharmacol. Exp. Ther. 2007, 323(1):294-307; Ng, H.J. et al., Proc.
Natl. Acad. Sci. U SA. 2007, 8;104(19):8059—64; Dinklo, T., J. Pharmacol. Exp. Ther. 2011, 336(2):560-74.). recites compounds of the overall ure Ar1\fl/N NZ R1 R2 which compounds are said to be PAMs of the o7 NNR.
The (17 NNR PAMs presently known generally trate weak activity, have a range of non-specific effects, or can only e limited access to the central nervous sys- tem where (17 NNRs are abundantly expressed. Accordingly, it would be beneficial to identify and provide new PAM compounds of (17 NNRs and compositions for treating diseases and disorders wherein o7 NNRs are involved. It would further be particularly beneficial if such compounds can e improved efficacy of treatment while reducing adverse effects asso- ciated with nds ing neuronal nicotinic receptors by selectively modulating o7 NNRs. s compounds of the overall structure R2 0 Y / (R)v N (Ci)q-(O)r-Ar p R5 R1 2/ ( R3)w as calcium or sodium channel blockers. Compound examples disclosed in are not intended to be included in the present invention.
Particularly the compounds (1 2-Phenyl-cyclopropanecarboxylic acid {(S)—1-[5- (2,2,2-trifluoro-ethoxy)—pyridinyl]—ethy|}-amide, (1 S,2S)(2-Chlorofluoro-phenyl)- cyclopropanecarboxylic acid {(S)—1-[5-(2,2,2-trifluoro-ethoxy)—pyridinyl]-ethyl}-amide and (1 S,2S)(2-F|uoromethoxy-phenyl)-cyc|opropanecarboxylic acid {(S)—1-[5-(2,2,2-trifluoroethoxy )-pyridinyl]-ethyl}-amide are disclosed in are disclaimed from the present invention [Annotation] sak SUMMARY OF THE INVENTION The objective of the t invention is to provide compounds that are positive allos- teric modulators (PAMs) of the nicotinic acetylcholine receptor subtype 017.
The compounds of the present invention are defined by formula [I] below: N \As H I / [L9 wherein R1, R2, R3, R4 and R5 are selected independently of each other from H, C1-5a|ky|, 02-5alkenyl, Cz_6alkynyl, C1_5alkoxy, cyano and halogen, wherein said C1_6alkyl, 02-5alkenyl and Cz_6alkynyl is optionally substituted with one or more substituents independently selected from chlorine and fluorine; R6 is selected from C1-6alkyl, 02-6alkenyl, kynyl and C1_6alkoxy, wherein said kyl, Cz_6alkenyl and Cz_6alkynyl is ally substituted with one or more substituents independ- ently selected from hydroxy, C1-5a|koxy and fluorine; A7 is C-R7 or N, A8 is C-R8 or N and A9 is C-R9 or N, provided that at least one of A7, A8 or and 9 is N and no more than two of A7, A8 and A9 is N; R7, R8, R9, R10 and R11 are selected independently of each other from H, C1_6alkyl, CZ- ealkenyl, Cz_6alkynyl, C1_6alkoxy, cyano, NR12R13, C1-5a|kylsulfony|, halogen and OR14, wherein said C1-5alky|, 02-5alkenyl, Cz_6alkynyl or C1-5a|koxy is optionally tuted with one or more substituents ed from chlorine, fluorine, koxy, cyano and NR12R13; R12 and R13 independently represent en, C1_6alkyl, 02-5alkenyl and Cz_6alkynyl; R14 represents a clic saturated ring moiety having 4-6 ring atoms wherein one of said ring atoms is O and the others are C; or R9 and R10 may be linked together to form the moiety indicated below O\l-/]n wherein n is 1, 2 or 3; [Annotation] sak and pharmaceutically acceptable salts thereof; with the proviso that the nd of formula [I] is other than (1 S,2S)Phenyl-cyclopropanecarboxylic acid {(S)—1-[5-(2,2,2-trifluoro-ethoxy)—pyridinyl]— ethy|}-amide; (1 S,2S)(2-Chlorofluoro-phenyl)-cyc|opropanecarboxylic acid -[5-(2,2,2-trifluoro- ethoxy)—pyridinyl]-ethyl}-amide; (1 S,2S)(2-F|uoromethoxy-phenyl)-cyc|opropanecarboxylic acid {(S)—1-[5-(2,2,2-trifluoro- ethoxy)—pyridinyl]-ethyl}-amide.
In one embodiment, the invention s to a compound ing to formula [I], and pharmaceutically acceptable salts thereof, for use as a medicament.
In one embodiment, the invention s to a compound according to a [I], and pharmaceutically acceptable salts thereof, for use in therapy.
In one embodiment, the invention relates to a compound according to formula [I], and pharmaceutically acceptable salts thereof, for use in the treatment of a disease or disorder selected from Psychosis; Schizophrenia; cognitive disorders; ive impairment associated with schizophrenia; Attention Deficit Hyperactivity Disorder (ADHD); autism spectrum disor- ders, Alzheimer’s disease (AD); mild cognitive impairment (MCI); age associated memory im- pairment (AAMI); senile dementia; AIDS dementia; Pick's disease; dementia ated with Lewy bodies; dementia associated with Down's syndrome; Huntington's Disease; Parkinson’s disease (PD); obsessive-compulsive disorder (OCD); traumatic brain injury; epilepsy; post- traumatic stress; Wernicke-Korsakoff syndrome (WKS); post-traumatic amnesia; cognitive deficits associated with depression; diabetes, weight control, inflammatory disorders, reduced angiogenesis; amyotrophic lateral sclerosis and pain.
In one ment, the invention relates to a pharmaceutical composition comprising a compound according to formula [I] and pharmaceutically acceptable salts f, and one or more pharmaceutically acceptable carrier or excipient.
In one embodiment, the invention relates to a kit comprising a compound ing to formula [I], and pharmaceutically acceptable salts thereof, together with a nd selected from the list consisting of acetylcholinesterase inhibitors; glutamate or antagonists; do- pamine transport inhibitors; noradrenalin transport inhibitors; D2 nists; D2 l ago- nists; PDE10 antagonists; 5-HT2A antagonists; 5-HT6 antagonists; KCNQ antagonists; lith- ium; sodium l blockers and GABA signaling enhancers.
In one embodiment, the invention relates to a method for the treatment of a disease or disorder selected from Psychosis; Schizophrenia; cognitive disorders; cognitive impairment associated with schizophrenia; Attention Deficit Hyperactivity er (ADHD); autism spec- trum disorders, Alzheimer’s disease (AD); mild cognitive impairment (MCI); age associated [Annotation] sak memory impairment (AAMI); senile dementia; AIDS dementia; Pick's disease; dementia asso- ciated with Lewy bodies; dementia associated with Down's syndrome; Huntington's Disease; Parkinson’s disease (PD); ive-compulsive disorder (OCD); traumatic brain injury; epi- lepsy; raumatic stress; Wernicke-Korsakoff syndrome (WKS); post-traumatic amnesia; cognitive deficits associated with depression; es, weight control, inflammatory disor- ders, reduced angiogenesis; amyotrophic lateral sclerosis and pain, which method comprises the administration of a therapeutically effective amount of a compound according to formula [I], and pharmaceutically acceptable salts thereof.
In one embodiment, the invention relates to the use of a compound according to for- mula [I], and pharmaceutically acceptable salts thereof, for the manufacture of a medicament for the ent of a disease or er selected from Psychosis; Schizophrenia; cognitive disorders; cognitive impairment associated with schizophrenia; Attention t Hyperactivity Disorder (ADHD); autism spectrum disorders, Alzheimer’s disease (AD); mild cognitive im- pairment (MCI); age ated memory impairment (AAMI); senile dementia; AIDS demen- tia; Pick's e; dementia associated with Lewy bodies; dementia associated with Down's syndrome; Huntington's Disease; Parkinson’s disease (PD); obsessive-compulsive disorder (OCD); traumatic brain injury; sy; post-traumatic stress; Wernicke-Korsakoff syndrome (WKS); post-traumatic amnesia; ive deficits associated with depression; diabetes, weight control, inflammatory disorders, reduced angiogenesis; amyotrophic lateral sclerosis and pain.
Definitions In the present context, “optionally substituted” means that the indicated moiety may or may not be substituted, and when substituted is mono-, di-, or tri-substituted, such as with 1, 2 or 3 substituents. In some instances, the substituent is independently selected from the group ting of C1_6alkyl, 02-5alkenyl, kynyl, phenyl, C1_5alkoxy, hydroxy and halo- gen. It is understood that where no substituents are indicated for an “optionally tuted” moiety, then the position is held by a hydrogen atom.
In the present context, “alkyl” is intended to indicate a straight, branched and/or cyclic saturated hydrocarbon. In ular “C1-6a|kyl” is intended to indicate such hydrocarbon hav- ing 1, 2, 3, 4, 5 or 6 carbon atoms. Examples of C1_6alkyl include methyl, ethyl, propyl, butyl, pentyl, hexyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, methylcyclopropyl, 2-methylpropyl and tert-butyl. Examples of substituted C1-5a|ky| include e.g. fluoromethyl and hy- droxymethyl.
In the present context, yl” is intended to te a omatic, straight, branched and/or cyclic arbon comprising at least one carbon-carbon double bond. In ation] sak particular “02-6alkenyl” is intended to indicate such hydrocarbon having 2, 3, 4, 5 or 6 carbon atoms. Examples of 02-5alkenyl e ethenyl, 1-propenyl, 2-propenyl, 1-butenyl, nyl and 3-butenyl and cyclohexenyl.
In the present t, “alkynyl” is intended to indicate a non-aromatic, straight, branched and/or cyclic hydrocarbon comprising at least one carbon-carbon triple bond and optionally also one or more carbon-carbon double bonds. In particular “02-6alkynyl” is in- tended to indicate such hydrocarbon having 2, 3, 4, 5 or 6 carbon atoms. Examples of CZ- ealkynyl include ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl and 5-but nyl.
In the present context, “hydroxy” is intended to indicate —OH.
In the present context, “alkoxy” is intended to indicate a moiety of the formula —OR’, wherein R’ indicates alkyl as defined above. In particular “C1-6a|koxy” is intended to indicate such moiety wherein the alkyl part has 1, 2, 3, 4, 5 or 6 carbon atoms. es of “C1- salkoxy” include methoxy, ethoxy, n-butoxy and tert-butoxy.
In the present context, “alkylsulfonyl” is intended to indicate —S(O)2alkyl In particular C1-5alkylsulfonyl is intended to indicate such a moiety wherein the alkyl part has 1, 2, 3, 4, 5 or 6 carbon atoms. Particular mention is made of methylsulfonyl.
In the present context, a “monocyclic moiety” is ed to cyclic moiety comprising only one ring, said cyclic moiety can be saturated or unsaturated.
In the present context, the terms “halo” and “halogen” are used interchangeably and refer to fluorine, ne, bromine or iodine.
In the t context, the term “cyano” indicates the group -CEN, which consists of a carbon atom triple-bonded to a nitrogen atom.
In the present context, ”ring atom” is intended to indicate the atoms constituting a ring, and ring atoms are selected from C, N, O and 8. As an example, benzene and e both have 6 carbons as ring atoms whereas pyridine has 5 carbons and 1 nitrogen as ring atoms.
In the t context, “heteroatom” means a nitrogen, oxygen or sulfur atom.
In the present context, “deuterium” indicates the atomic isotope of hydrogen consisting of one proton and one neutron in its nucleus, and thus having an approximate weight of two (2). Deuterium is represented as D, d or 2H. An example of a substituent comprising deute- rium is -d3)-ethoxy wherein three of the hydrogens in ethoxy are the 2H isotopes.
In the present context, “enantiomeric excess” represents the % excess of a compound in a mixture of compound enantiomers. If for example an omeric excess is 90% then the ratio of the compound to its enantiomer is 95:5 and if an enantiomeric excess is 95% then the ratio of the nd to its enantiomer is 97.525 Likewise, “diastereomeric ” represents % excess of a compound in a mixture of compound diastereomers.
[Annotation] sak In the t context, pharmaceutically acceptable salts include pharmaceutically acceptable acid addition salts, pharmaceutically acceptable metal salts, ammonium and alky- lated ammonium salts. Acid addition salts include salts of inorganic acids as well as organic acids. es of suitable inorganic acids include hydrochloric, hydrobromic, hydroiodic, phosphoric, sulfuric, ic, nitric acids and the like.
Examples of suitable organic acids include formic, acetic, trichloroacetic, trifluoroace- tic, nic, benzoic, cinnamic, citric, fumaric, glycolic, itaconic, lactic, esulfonic, maleic, malic, malonic, mandelic, oxalic, picric, c, salicylic, succinic, methane sulfonic, ethanesulfonic, tartaric, ascorbic, pamoic, bismethylene salicylic, ethanedisulfonic, gluconic, citraconic, aspartic, stearic, palmitic, EDTA, glycolic, p-aminobenzoic, glutamic, benzenesul- fonic, p-toluenesulfonic acids, theophylline acetic acids, as well as the 8—halotheophyllines, for example 8—bromotheophylline and the like. Further examples of ceutical acceptable inorganic or c acid addition salts include the pharmaceutically acceptable salts listed in Berge, SM. et al., J. Pharm. Sci. 1977,66,2, which is incorporated herein by reference. es of metal salts include lithium, sodium, potassium, magnesium salts and the like.
Examples of ammonium and alkylated ammonium salts include ammonium, methyl-, dimethyl-, trimethyl-, ethyl-, hydroxyethyl-, l-, n-butyl-, sec-butyl-, tert-butyl-, tetrame- thylammonium salts and the like.
In the present context, pharmaceutical carriers include inert solid diluents or fillers, sterile aqueous solutions and various organic solvents. es of solid carriers include lac- tose, terra alba, sucrose, cyclodextrin, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid and lower alkyl ethers of cellulose. Examples of liquid carriers include, but are not limited to, syrup, peanut oil, olive oil, phospholipids, fatty acids, fatty acid , poly- oxyethylene and water. Similarly, the carrier may include any sustained release material known in the art, such as glyceryl monostearate or glyceryl distearate, alone or mixed with a wax.
In the present context, the term "therapeutically ive " of a nd means an amount sufficient to cure, alleviate or partially arrest the al manifestations of a given disease and its complications in a eutic intervention comprising the administra- tion of said compound. An amount adequate to accomplish this is defined as "therapeutically effective amount". Effective amounts for each purpose will depend on the severity of the dis- ease or injury as well as the weight and l state of the subject. It will be understood that determining an appropriate dosage may be achieved using routine experimentation, by con- [Annotation] sak structing a matrix of values and testing different points in the matrix, which is all within the or- dinary skills of a trained ian.
In the present context, the term ment" and "treating" means the management and care of a patient for the purpose of combating a condition, such as a disease or a disor- der. The term is intended to include the full spectrum of treatments for a given condition from which the patient is ing, such as administration of the active compound to alleviate the symptoms or complications, to delay the progression of the disease, disorder or condition, to alleviate or relief the symptoms and complications, and/or to cure or eliminate the disease, er or condition as well as to prevent the condition, wherein prevention is to be under- stood as the ment and care of a patient for the purpose of combating the e, condition, or disorder and includes the administration of the active compounds to prevent the onset of the symptoms or complications. eless, prophylactic (preventive) and thera- peutic (curative) treatments are two separate aspects of the present invention. The patient to be d is ably a , in particular a human being.
In the present context, the term "cognitive disorders" is intended to indicate disorders characterized by abnormalities in aspects of perception, problem solving, language, learning, working memory, memory, social recognition, attention and pre-attentional processing, such as by not limited to Attention t Hyperactivity Disorder (ADHD), autism spectrum disor- ders, Alzheimer’s e (AD), mild cognitive impairment (MCI), age associated memory im- pairment (AAMI), senile dementia, vascular dementia, frontotemporal lobe dementia, Pick's disease, dementia ated with Lewy bodies, and dementia associated with Down's syn- drome, cognitive impairment associated with Multiple Sclerosis, cognitive impairment in epi- lepsy, cognitive ment associated with fragile X, cognitive impairment associated with neurofibromatosis, cognitive impairment associated with Friedreich’s Ataxia, progressive su- pranuclear palsy (PSP), HIV associated dementia (HAD), HIV associated cognitive impair- ment (HIV-Cl), gton's Disease, Parkinson’s e (PD), obsessive-compulsive dis- order (OCD), traumatic brain injury, epilepsy, post-traumatic stress, Wernicke-Korsakoff syn- drome (WKS), post-traumatic amnesia, cognitive deficits associated with depression as well as ive impairment associated with schizophrenia.
The cognitive enhancing properties of a compound can be assessed eg. by the atten- tional set-shifting paradigm which is an animal model allowing assessment of executive func- tioning via intra-dimensional (lD) versus extra-dimensional (ED) shift discrimination ng.
The study can be performed by testing whether the compound is attenuating tional per- formance impairment” induced by subchronic PCP administration in rats as described by Rodefer, J.S. et al., Eur. J. Neurosci. 2005, 21:1070-1076.
[Annotation] sak In the present t, the term "autism spectrum disorders" is intended to te disorders characterized by widespread alities of social interactions and verbal and non-verbal communication, as well as restricted interests, repetitive behavior and attention, such as by not limited to autism, Asperger syndrome, Pervasive Developmental Disorder Not Otherwise Specified (PDD-NOS), Rett syndrome, Angelmann syndrome, e X, DiGeorge syndrome and Childhood Disintegrative Disorder.
In the present context, the term "inflammatory disorders” is intended to indicate disor- ders characterized by abnormalities in the immune system such as by not limited to, ic reactions and myopathies resulting in abnormal inflammation as well as non-immune diseases with etiological origins in inflammatory ses are thought to include but not be limited to cancer, atherosclerosis, osteoarthritis, rheumatoid arthritis and ischaemic heart disease.
DETAILED DESCRIPTION OF THE INVENTION The present inventors have found that certain new compounds are ve allosteric modulators (PAMs) of NNRs, and as such may be used in the treatment of various ers.
PAMs of NNRs may be dosed in combination with other drugs in order to achieve more efficacious treatment in certain patient populations. An (17 NNR PAM may act synergis- tically with another drug, this has been described in animals for the combination of com- pounds affecting nicotinic receptors, including (17 NNRs and D2 antagonism (Wiker, 0., Int. J.
Neuropsychopharmacol. 2008, 11(6):845-50).
Thus, compounds of the present invention may be useful treatment in the combination with another drug e.g. selected from acetylcholinesterase inhibitors, glutamate receptor an- tagonists, dopamine transport inhibitors, noradrenalin transport inhibitors, D2 antagonists, D2 partial agonists, PDE10 nists, 5-HT2A antagonists, 5-HT6 antagonists and KCNQ an- tagonists, lithium, sodium channel blockers, GABA signalling enhancers.
In one ment, compounds of the present invention are used for ent of pa- tients who are already in treatment with another drug ed from the list above. In one em- bodiment, compounds of the present invention are adapted for administration simultaneous with said other drug. In one embodiment compounds of the present invention are d for administration sequentially with said other drug. In one ment, compounds of the pre- sent invention are used as the sole medicament in treatment of a patient. In one embodiment, compounds of the present ion are used for treatment of patients who are not already in treatment with r drug selected from the list above.
Embodiments according to the invention [Annotation] sak In the following, embodiments of the invention are disclosed. The first embodiment is denoted E1, the second embodiment is denoted E2 and so forth.
E1. A compound according to formula [I] N \As H I / [L9 wherein R1, R2, R3, R4 and R5 are selected independently of each other from H, C1-5alkyl, 02-5alkenyl, Cz_6alkynyl, C1_5alkoxy, cyano and halogen, wherein said C1_6alkyl, 02-5alkenyl and Cz_6alkynyl is optionally substituted with one or more substituents independently selected from chlorine and fluorine; R6 is selected from C1-6a|ky|, Cz_6a|keny|, Cz_6a|kyny| and C1_6alkoxy, wherein said C1_6a|ky|, keny| and Cz_6alkynyl is optionally substituted with one or more substituents independ- ently selected from hydroxy, C1-5alkoxy and ne; A7 is C-R7 or N, A8 is C-R8 or N and A9 is C-R9 or N, ed that at least one of A7, A8 or and 9 is N and no more than two of A7, A8 and A9 is N; R7, R8, R9, R10 and R11 are selected independently of each other from H, C1_6alkyl, CZ- ealkenyl, Cz_6alkynyl, C1_6alkoxy, cyano, NR12R13, C1-5alkylsulfonyl, n and OR14, wherein said C1-5alkyl, 02-5alkenyl, Cz_6alkynyl or C1_6alkoxy is optionally substituted with one or more substituents selected from chlorine, ne, C1_6alkoxy, cyano and NR12R13; R12 and R13 independently represent en, C1_6alkyl, 02-5alkenyl and Cz_6alkynyl; R14 represents a monocyclic ted ring moiety having 4-6 ring atoms n one of said ring atoms is O and the others are C; or R9 and R10 may be linked together to form the moiety indicated below O\l-/]n wherein n is 1, 2 or 3; and pharmaceutically acceptable salts thereof; [Annotation] sak with the proviso that the compound of formula [I] is other than (1 S,28)Phenyl-cyclopropanecarboxylic acid {(S)—1-[5-(2,2,2-trifluoro-ethoxy)—pyridinyl]— ethy|}-amide; (1 S,28)(2-Chlorofluoro-phenyl)-cyclopropanecarboxylic acid {(S)—1-[5-(2,2,2-trifluoroethoxy )—pyridinyl]-ethyl}-amide; (1 S,28)(2-F|uoromethoxy-phenyl)-cyclopropanecarboxylic acid {(S)—1-[5-(2,2,2-trifluoro- ethoxy)—pyridinyl]-ethyl}-amide.
E2. The compound ing to embodiment 1, wherein R1, R2, R3, R4 and R5 are se- lected independently of each other from H, methyl, fluorine and chlorine; R6 is selected from methyl, hydroxymethyl, methoxymethyl and fluoromethyl; R7, R8, R9, R10 and R11 are selected independently of each other from H, C1_4alkyl, C1- 4alkoxy, cyano, -N(CH3)2, methylsulfonyl, fluorine, chlorine and OR14, n said C1_4alkyl or C1.4a|koxy is ally substituted with one or more substituents selected from fluorine, C1- 4a|koxy and cyano; R14 represents a monocyclic saturated ring moiety having 4-6 ring atoms wherein one of said ring atoms is O and the others are C; or R9 and R10 may be linked together to form the moiety indicated below O\l-/]n wherein n is 1 or 2.
E3. The compound ing to any of ments 1-2, n R1, R2, R3, R4 and R5 are selected independently of each other from H, methyl, fluorine and chlorine.
E4. The compound according to any of embodiments 1-3, wherein four or more of R1, R2, R3, R4 and R5 are H.
E5. The compound according to embodiment 4, wherein all of R1, R2, R3, R4 and R5 are E6. The compound according to any of embodiments 1-5, wherein R6 is selected from methyl , hydroxymethyl, methoxymethyl and fluoromethyl.
[Annotation] sak E7. The compound according to ment 6, wherein R6 is methyl.
E8. The compound according to embodiment 6, wherein R6 is hydroxymethyl.
E9. The compound according to ment 6, wherein R6 is ymethyl.
E10. The compound ing to embodiment 6, wherein R6 is fluoromethyl.
E11. The compound according to any of ments 1-10, wherein R7, R8, R9, R10 and R11 are selected independently of each other from H, C1_4alkyl, C1_4alkoxy, cyano, -N(CH3)2, methylsulfonyl, fluorine, chlorine and OR14, wherein said C1_4alkyl or C1_4alkoxy is optionally substituted with one or more substituents selected from fluorine, koxy and cyano; R14 represents a monocyclic saturated ring moiety having 4-6 ring atoms wherein one of said ring atoms is O and the others are C; or R9 and R10 may be linked together to form the moiety indicated below O\l-/]n wherein n is 1 or 2; E12. The compound according to any of embodiments 1 and 3-11, wherein R7, R8, R9, R10 and R11 are selected independently from H, C1_6alkyl, 02-5alkenyl, 02.6alkynyl, C1_6alkoxy, cyano or halogen, wherein said C1_6alkyl, 02-6alkenyl, 02-6alkynyl or C1_6alkoxy is optionally substituted with one or more substituents selected from fluorine, C1.6alkoxy and cyano.
E13. The compound according to any of embodiments 1-12, wherein R7, R8, R9, R10 and R11 are selected independently from H, C1_4alkyl, Cz_4alkenyl, Cz_4alkynyl, koxy, cyano and halogen, wherein said C1_4alkyl, Cz_4alkenyl, Cz_4alkynyl or koxy is optionally substi- tuted with one or more tuents selected from fluorine and C1_4alkoxy.
E14. The compound according to any of embodiments 1-13, wherein one or more of the hydrogen atoms are represented by deuterium.
E15. The compound according to embodiment 14, wherein one or more of the hydrogen atoms in R7, R8, R9, R10 and R11 are represented by deuterium. ation] sak E16. The compound according to any of embodiments 14-15, wherein at least about 85% of the compound has a deuterium atom at each position designated as ium, and any atom not designated as deuterium is present at about its natural isotopic abundance.
E17. The compound according to embodiment 16, wherein at least about 90% of the com- pound has a deuterium atom at each position designated as deuterium, and any atom not designated as deuterium is present at about its l isotopic abundance.
E18. The compound according to any of embodiments 1-17, wherein no more than one of A7, A8 or A9 is N.
E19. The nd according to any of embodiments 1-18, wherein A7 is N, A8 is C-R8 and A9 is C-R9.
E20. The compound according to embodiment 19, wherein R8, R10 and R11 all represent E21. The compound according to any of embodiments 1-18, wherein A8 is N, A7 is C-R7 and A9 is C-R9.
E22. The compound ing to embodiment 21, wherein R7, R10 and R11 all represent E23. The compound according to any of embodiments 19-22, wherein R9 is selected from methyl, C1_4alkoxy or cyano, n said methyl is optionally substituted with C1_4alkoxy or one or more fluorine.
E24. The compound according to embodiment 23, wherein R9 represents koxy and one or more of the hydrogen atoms in said C1.4alkoxy are represented by deuterium.
E25. The compound according to any of embodiments 19-22, wherein R9 is OR14, wherein R14 represents a monocyclic saturated ring moiety having 4-6 ring atoms wherein one of said ring atoms is O and the others are C.
[Annotation] sak E26. The compound according to any of embodiments 1-18, wherein A9 is N, A7 is C-R7 and A8 is C-R8.
E27. The compound according to embodiment 26, wherein R7, R8 and R11 all represent H.
E28. The nd according to any of embodiments 26-27, wherein R10 is selected from methyl, C1_4alkoxy or cyano, wherein said methyl is optionally substituted with C1_4alkoxy or one or more fluorine.
E29. The compound according to embodiment 28, wherein R10 represents koxy and one or more of the hydrogen atoms in said C1.4a|koxy are ented by deuterium.
E30. The compound according to any of embodiments 1-17, wherein two of A7, A8 or A9 are N.
E31. The compound according to any of embodiments 1-30 having a diastereomeric ex- cess of at least 80% such as at least 85%, such as at least 90%, such as at least 95%.
E32. The compound according to embodiment 1 ed from 1 : (1 S, Phenyl—cyclopropanecarboxylic acid [(S)(6-isopropoxy—pyridinyl)-ethyl]— amide; 2: (1 S, 2S)Phenyl—cyclopropanecarboxylic acid [(S)(5-methyl—pyridiny/)-ethyl]—amide; 3: (1 S, 28)Phenyl—cyclopropanecarboxylic acid [(S)(6-methoxy—pyridinyl)-ethy/]—amide; 4: (1 S, 2S)Phenyl—cyclopropanecarboxylic acid [(S)(6-methyl—pyridin-3—y/)-ethyl]—amide; 5: (1 S, 2S)-2—Phenyl—cyclopropanecarboxylic acid [(S)(6-cyano-pyridinyl)-ethyl]—amide; 6: (1 S, Phenyl—cyclopropanecarboxylic acid [(S)(6-trifluoromethyl—pyridinyl)-ethyl]- amide; 7: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(S)(6-ethoxy-pyridin-3—y/)-ethyl]—amide; 8: (1 S, 28)Phenyl—cyclopropanecarboxylic acid [(S)(6-ethyl-pyridinyl)-ethy/]—amide; 9: (1 S, 28)Phenyl—cyclopropanecarboxylic acid [(S)(6-methoxymethyl-pyridinyl)-ethyl]— amide; 1 0: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid {(S)-1 -[6-(2, 2, 2-trifluoro-ethoxy)-pyridin-3— y/]-ethyl}-amide; 1 1 : (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(S)[6-(2-methoxy-ethoxy)-pyridinyl]— -amide; 12: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(S)(2-ethoxy-pyridiny/)-ethyl]—amide; [Annotation] sak 13: (1 S, 2S)Phenyl-cyclopropanecarboxy/ic acid ((S){6-[(S)—(tetrahydro-furanyl)oxy]- pyridiny/}-ethyl)—amide; 14: (1 S, 2S)Phenyl-cyclopropanecarboxy/ic acid ((S){6-[(R)—(tetrahydro-furanyl)0ny- pyridinyl}-ethyl)-amide; : (1 S, 28)-2—Phenyl—cyclopropanecarboxy/ic acid -[1, 3]dioxo/o[4, 5-b]pyridinyl—ethyl)- amide; 16: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(S)(2, 3-dihydro-[1, ino[2, 3- b]pyridin- 7-y/)-ethyl]—amide; 17: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(S)(2-ethoxy-pyrimidiny/)-ethyl]— amide; 1 8: (1 S, 2S)Phenyl-cyclopropanecarboxy/ic acid [(S)(6-chloro-pyridiny/)-ethyl]—amide; 19: (1 S, 2S)Phenyl-cyclopropanecarboxy/ic acid {(8)[6-(oxetanyloxy)—pyridinyl]— ethyl}-amide; : (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(S)(6-cyanomethoxy-pyridinyl)— ethy/J-amide; 21 : (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(R)hydroxy(6-propoxy-pyridiny/)- ethy/J-amide; 22: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(R)hydroxy(6-trifluoromethyl-pyridin- 3-yl)-ethy/]—amide; 23: (1 S, 28)-2—Phenyl—cyclopropanecarboxy/ic acid -(5-cyano-pyridin-2—yl)—2—hydroxy- ethy/J-amide; 24: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid -hydroxy(6-methoxy-pyridinyl)— ethy/J-amide; : (1 S, 28)-2—Phenyl—cyclopropanecarboxy/ic acid [(R)hydroxy(6-methyl—pyridinyl)— ethy/J-amide; 26: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(R)hydroxy(6-isopropoxy-pyridin y/)-ethy/]-amide; 27: (1 S, 2S)(3-Fluoro-phenyl)-cyclopropanecarboxy/ic acid [(R)(6-ethoxy-pyridiny/)—2— hydroxy-ethy/]—amide; 28: (1 S, 2S)(4-Fluoro-phenyl)-cyclopropanecarboxy/ic acid [(R)(6-ethoxy-pyridiny/)—2— hydroxy-ethy/]—amide; 29: (1 S, (3-F/uoro-phenyl)—cyclopropanecarboxy/ic acid [(R)hydroxy(6-propoxy- pyridiny/)-ethyl]—amide; : (1 S, 2S)-2—(4-F/uoro-phenyl)—cyclopropanecarboxy/ic acid [(R)hydroxy(6-propoxy- pyridiny/)-ethyl]—amide; [Annotation] sak 31 : (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(R)-1 -(6-(2, 2, 2-d3)-ethoxy-pyridin-3—yl)—2- hydroxy-ethy/]—amide,' 32: (1 S, 2S)(3-Fluoro-phenyl)-cyclopropanecarboxy/ic acid [(R)(6-(1, 1 -d2)-ethoxy-pyridin- 3—yl)hydroxy-ethy/]—amide,' 33: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(R)(6-ethoxy-pyridin-3—y/)hydroxy- ethy/J-amide; 34: (1 S, 2S)(4-Fluoro-phenyl)-cyclopropanecarboxy/ic acid [(R)(6-(1, 1, 2, 2, 2-d5)-ethoxy- pyridinyl)hydroxy-ethyl]—amide,' : (1 S, 2S)(4-Fluoro-phenyl)-cyclopropanecarboxy/ic acid [(R)-1 -(6-(2, 2, 2-d3)-ethoxy- pyridinyl)hydroxy-ethyl]—amide,' 36: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(R)(6-(1, 1 -d2)-ethoxy-pyridin-3—yl)—2- hydroxy-ethy/]—amide,' 37: (1 S, 2S)(4-Fluoro-phenyl)-cyclopropanecarboxy/ic acid [(R)(6-(1, 1 -d2)-ethoxy-pyridin- 3—yl)hydroxy-ethy/]—amide,' 38: (1 S, Phenyl—cyclopropanecarboxy/ic acid [(R)(6-(1, 1, 2, 2, ethoxy-pyridin hydroxy-ethy/J—amide,‘ 39: (1 S, 2S)(3-F/uoro-phenyl)—cyclopropanecarboxy/ic acid [(R)(6-(2, 2, 2-d3)-ethoxy- pyridinyl)hydroxy-ethyl]—amide,' 40: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(R)(6-cyc/obutoxy-pyridin-3—yl)—2- hydroxy-ethy/]—amide,' 41 : (1 S, 2S)(3-F/uoro-phenyl)—cyclopropanecarboxy/ic acid -(6-cyc/obutoxy-pyridin-3— y/)hydroxy-ethy/J—amide,‘ 42: (1 S, 2S)(4-F/uoro-phenyl)-cyc/opropanecarboxy/ic acid -(6-cyc/obutoxy-pyridin-3— y/)hydroxy-ethy/J—amide,‘ 43: (1 S, 2S)Phenyl—cyclopropanecarboxylic acid ((R)hydroxy{6-[(R)-(tetrahydro-furan- 3—yl)oxy]-pyridin-3—yl}-ethy/)-amide,' 44: (1 S, [(1 R)hydroxy[6-[(38)-tetrahydrofuran-3—y/Joxypyridyljethyl]—2-phenyl— cyc/opropanecarboxamide,‘ 45: (1 S, 28)—2-((Z)Methy/ene—pem‘a-2, 4-dienyl)-cyclopropanecarboxy/ic acid {(R) hydroxy[6-(tetrahydro-pyranyloxy)—pyridiny/]-ethy/}-amide,' 46: (1 S, 2S)Phenyl—cyc/opropanecarboxylic acid [(R)(6-ethoxy-pyridin-3—y/)methoxy- ethy/J-amide; 47: (1 S, 2S)-N-[(1R)—2-methoxy[6-[(3R)-tetrahydrofuran-3—yl]oxy-3—pyridyl]ethy/J—2-phenyl- cyc/opropanecarboxamide,‘ 48: (1 S, 2S)-N-[(1R)—2-methoxy[6-[(3S)-tetrahydrofuran-3—yl]oxy-3—pyridy/]ethy/J—2-phenyl— cyc/opropanecarboxamide,‘ [Annotation] sak 49: (1 S, 2S)-2—Phenyl—cyclopropanecarboxylic acid {(R)-2—methoxy[6-(tetrahydro-pyran yloxy)-pyridinyl]-ethyl}-amide; 50: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid {(8)[6-(oxetanyloxy)-pyridinyl]— ethyl}-amide; 51 : (1 S, 2S)Phenyl-cyclopropanecarboxy/ic acid [(S)(6-ethanesulfonyl -pyridinyl)-ethyl]—amide; 52: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(R)(5-ethoxy-pyridin- 2-hydroxy-ethy/]—amide; and pharmaceutically acceptable salts of any of these compounds.
E33. A nd according to any of embodiments 1-32, for use as a medicament.
E34. A compound according to any of embodiments 1-32, for use in therapy.
E35. A compound ing to any of embodiments 1-32, for use in the treatment of a dis- ease or disorder selected from Psychosis; Schizophrenia; ive disorders; cognitive im- pairment associated with schizophrenia; Attention Deficit Hyperactivity Disorder ; au- tism spectrum disorders, Alzheimer’s disease (AD); mild cognitive impairment (MCI); age as- sociated memory ment (AAMI); senile ia; AIDS dementia; Pick's disease; de- mentia associated with Lewy bodies; dementia associated with Down's syndrome; Hunting- ton's Disease; Parkinson’s disease (PD); obsessive-compulsive disorder (OCD); traumatic brain injury; epilepsy; post-traumatic stress; Wernicke-Korsakoff syndrome (WKS); post- traumatic amnesia; ive deficits associated with depression; diabetes, weight control, matory disorders, reduced angiogenesis; ophic lateral sclerosis and pain.
E36. The compound according to embodiment 35, wherein said a disease or disorder is selected from schizophrenia; AD; ADHD; autism spectrum disorders; PD; amyotrophic lateral sclerosis; Huntington's disease; dementia associated with Lewy bodies and pain.
E37. The compound according to embodiment 36, wherein said e or disorder is se- lected from schizophrenia; AD; ADHD and autism spectrum disorders.
E38. The compound according to embodiment 37, wherein said disease or disorder is se- lected from negative and/or ive symptoms of schizophrenia.
[Annotation] sak E39. The compound ing to any of embodiments 1-32, for use concomitantly or se- quentially with a therapeutically ive amount of a compound selected from the list consisting of acetylcholinesterase inhibitors; glutamate receptor antagonists; dopamine transport inhibitors; noradrenalin transport inhibitors; D2 antagonists; D2 partial agonists; PDE10 an- tagonists; 5-HT2A antagonists; 5-HT6 antagonists; KCNQ antagonists; lithium; sodium chan- nel blockers and GABA signaling enhancers in the treatment of a disease or disorder accord- ing to any of embodiments 35-38.
E40. A pharmaceutical composition comprising a compound according to any of embodi- ments 1-32, and one or more pharmaceutically acceptable carrier or excipient.
E41. The composition according to embodiment 40, which composition additionally com- prises a second compound selected from the list consisting of acetylcholinesterase inhibitors; glutamate receptor antagonists; dopamine transport inhibitors; noradrenalin transport inhibi- tors; D2 antagonists; D2 l ts; PDE10 antagonists; 5-HT2A antagonists; 5-HT6 antagonists; KCNQ antagonists; m; sodium channel blockers and GABA signaling en- hancers.
E42. The composition according to embodiment 41, wherein said second compound is an acetylcholinesterase inhibitor.
E43. A kit comprising a nd according to any of embodiments 1-32, together with a second compound selected from the list consisting of cholinesterase inhibitors; gluta- mate receptor antagonists; dopamine transport inhibitors; noradrenalin transport inhibitors; D2 antagonists; D2 partial agonists; PDE10 antagonists; 5-HT2A antagonists; 5-HT6 antago- nists; KCNQ nists; m; sodium l blockers and GABA signaling enhancers.
E44. The kit according to embodiment 43, wherein said second compound is an acetylcho- linesterase inhibitor.
E45. A method for the treatment of a disease or disorder selected from Psychosis; Schizo- phrenia; cognitive disorders; cognitive impairment ated with schizophrenia; Attention Deficit Hyperactivity Disorder (ADHD); autism spectrum disorders, Alzheimer’s disease (AD); mild ive impairment (MCI); age associated memory impairment (AAMI); senile demen- tia; AIDS dementia; Pick's disease; ia associated with Lewy bodies; dementia - ated with Down's syndrome; gton's Disease; Parkinson’s disease (PD); obsessive- [Annotation] sak compulsive disorder (OCD); traumatic brain injury; epilepsy; post-traumatic stress; Wernicke- Korsakoff syndrome (WKS); post-traumatic a; cognitive deficits associated with de- pression; diabetes, weight control, matory disorders, reduced angiogenesis; amyotro- phic lateral sclerosis and pain, which method comprises the administration of a therapeuti- cally effective amount of a compound according to any of embodiments 1-32 to a patient in need thereof.
E46. The method according to embodiment 45, wherein said disease or disorder is selected from schizophrenia; AD; ADHD; autism spectrum disorders; PD; amyotrophic lateral sclerosis; Huntington's e; dementia associated with Lewy bodies and pain.
E47. The method according to embodiment 46, wherein said disease or disorder is ed from schizophrenia; AD; ADHD and autism spectrum disorders.
E48. The method according to embodiment 47, wherein said treatment comprises the treat- ment of negative and/or cognitive symptoms of schizophrenia.
E49. The method according to any of embodiments 45-48, wherein said treatment further comprises the administration of a therapeutically effective amount of a second compound se- lected from the list consisting of acetylcholinesterase inhibitors; glutamate receptor antago- nists; dopamine transport inhibitors; noradrenalin transport inhibitors; D2 nists; D2 partial agonists; PDE10 nists; 5-HT2A antagonists; 5-HT6 antagonists; KCNQ - nists; lithium; sodium channel blockers and GABA signaling enhancers.
E50. The method according to embodiment 49, wherein said second compound is an ace- tylcholinesterase inhibitor.
E51. Use of a compound ing to any of embodiments 1-32, for the manufacture of a medicament for the treatment of a disease or er selected from sis; Schizophre- nia; cognitive disorders; cognitive impairment associated with schizophrenia; Attention Deficit Hyperactivity Disorder (ADHD); autism spectrum disorders, Alzheimer’s disease (AD); mild cognitive impairment (MCI); age associated memory ment (AAMI); senile dementia; AIDS dementia; Pick's disease; dementia ated with Lewy bodies; dementia associated with Down's syndrome; Huntington's Disease; Parkinson’s disease (PD); obsessive- compulsive disorder (OCD); traumatic brain injury; epilepsy; post-traumatic ; ke- Korsakoff syndrome (WKS); post-traumatic amnesia; cognitive ts associated with de- [Annotation] sak pression; diabetes, weight control, inflammatory disorders, reduced angiogenesis; amyotro- phic lateral sclerosis and pain.
E52. The use according to embodiment 51, wherein said disease or disorder is selected from schizophrenia; AD; ADHD; autism um disorders; PD; amyotrophic lateral sis; Huntington's disease; dementia associated with Lewy bodies and pain.
E53. The use according to embodiment 52, n said e or disorder is ed from schizophrenia; AD; ADHD and autism spectrum disorders.
E54. The use according to embodiment 53, wherein said disease is the positive, negative and/or ive symptoms of schizophrenia.
E55. The use according to any of embodiments 51-54, wherein said manufacture r comprises the use of a second compound ed from the list consisting of acetylcholi- nesterase inhibitors; glutamate receptor antagonists; dopamine transport inhibitors; noradrenalin ort inhibitors; D2 antagonists; D2 partial agonists; PDE10 antagonists; 5- HT2A antagonists; 5-HT6 antagonists; KCNQ antagonists; lithium; sodium channel blockers and GABA signaling enhancers.
E56. The use ing to embodiment 55, wherein said second compound is an acetyl- cholinesterase inhibitor.
The compounds of the invention may exist in unsolvated as well as in solvated forms in which the solvent molecules are selected from pharmaceutically acceptable solvents such as water, l and the like. In general, such solvated forms are considered equivalent to the unsolvated forms for the purposes of this invention.
Included in this invention are also isotopically labeled compounds, which are identical to those claimed in formula [I], wherein one or more atoms are represented by an atom of the same element having an atomic mass or mass number different from the atomic mass or mass number usually found in nature (e.g., 2H, 3H, 11C, 130, 15N, 18F and the like). Particular mention is made of 2H substituted compounds i.e. compounds wherein one or more H atoms are represented by deuterium. In one ment of the invention one or more of the hydro- gen atoms of the compound of a [I] are represented by deuterium. It is ized that elements are present in natural isotopic abundances in most synthetic compounds, and result in inherent incorporation of deuterium. However, the natural isotopic abundance of hydrogen [Annotation] sak isotopes such as deuterium is immaterial (about 0.015%) ve to the degree of stable iso- topic substitution of compounds indicated herein. Thus, as used herein, designation of an atom as deuterium at a position indicates that the abundance of deuterium is significantly greater than the natural abundance of deuterium. Any atom not ated as a particular isotope is intended to represent any stable isotope of that atom, as will be apparent to the or- dinarily skilled artisan.
In one embodiment, designation of a position as “D” in a compound has a minimum deuterium incorporation of greater than about 60% at that position such as greater than about 70% at that on such as greater than about 80% at that position such as greater than about 85% at that position. In a further embodiment, designation of a on as “D” in a compound has a minimum deuterium incorporation of greater than about 90% at that position such as greater than about 95% at that on such as greater than about 97% at that posi- tion such as greater than about 99% at that position.
The compounds of the t invention have three asymmetric centers with fixed stereochemistry indicated by the arrows below.
The compounds of the present invention can be manufactured from two chiral inter- mediates with one and two asymmetric centers, respectively, as rated by the examples below.
In this context is understood that when specifying the enantiomeric form of the inter- e, then the intermediate is in enantiomeric excess, e.g. essentially in a pure, mono- enantiomeric form. Accordingly, the resulting compounds of the invention are having a di- astereomeric excess of at least 80%. One embodiment of the invention relates to a com- pound of the invention having a diastereomeric excess of at least 80% such as at least 85%, such as at least 90%, ably at least 95% or at least 97% with reference to the three as- symetric centers ted above.
Dependent on the individually substituents R1-R14, the nds of the t in- vention may furthermore have one or more additional asymmetric centers. It is intended that any optical isomers (i.e. enantiomers or diastereomers), in the form of separated, pure or par- tially purified optical isomers and any es thereof including racemic mixtures, Le. a mix- ture of stereoisomers, which have emerged because of asymmetric centers in any of sub- stituents R1-R14, are included within the scope of the invention.
[Annotation] sak Racemic forms can be resolved into the optical antipodes by known methods, for ex- ample by separation of reomeric salts f with an optically active acid, and liberat- ing the optically active amine compound by ent with a base. Another method for - ing racemates into the optical antipodes is based upon chromatography of an optically active matrix. The compounds of the present invention may also be resolved by the formation of di- astereomeric derivatives. Additional methods for the resolution of optical isomers, known to those skilled in the art, may be used. Such methods include those discussed by J. Jaques, A.
Collet and S. Wilen in “Enantiomers, Racemates, and Resolutions”, John Wiley and Sons, New York . Optically active compounds can also be prepared from optically active starting materials.
Furthermore, when a double bond or a fully or partially saturated ring system is pre- sent in the molecule geometric isomers may be formed. It is intended that any geometric iso- mers, as separated, pure or lly ed geometric isomers or mixtures thereof are in- cluded within the scope of the invention. Likewise, molecules having a bond with restricted rotation may form geometric isomers. These are also intended to be included within the scope of the present invention.
Furthermore, some of the compounds of the present invention may exist in different tautomeric forms and it is intended that any tautomeric forms that the compounds are able to form are included within the scope of the present invention.
The compounds of the present ion may be administered alone as a pure com- pound or in combination with ceutically acceptable rs or excipients, in either sin- gle or multiple doses. The pharmaceutical compositions according to the invention may be formulated with pharmaceutically acceptable carriers or diluents as well as any other known adjuvants and ents in accordance with conventional techniques such as those disclosed in Remington: The Science and Practice of Pharmacy, 19 Edition, Gennaro, Ed., Mack Pub- lishing 00., Easton, PA, 1995.
The pharmaceutical compositions may be specifically formulated for administration by any suitable route such as the oral, rectal, nasal, pulmonary, l (including buccal and sublingual), transdermal, intracisternal, intraperitoneal, vaginal and eral (including sub- cutaneous, intramuscular, intrathecal, intravenous and intradermal) route, the oral route being preferred. It will be appreciated that the preferred route will depend on the general condition and age of the subject to be d, the nature of the condition to be treated and the active ingredient chosen.
Pharmaceutical compositions for oral administration include solid dosage forms such as capsules, tablets, dragees, pills, lozenges, powders and granules. Where riate, they can be prepared with coatings.
[Annotation] sak Liquid dosage forms for oral administration include solutions, emulsions, suspensions, syrups and elixirs.
Pharmaceutical itions for parenteral administration include sterile aqueous and nonaqueous injectable solutions, dispersions, suspensions or emulsions as well as sterile powders to be reconstituted in sterile injectable solutions or dispersions prior to use.
Other suitable administration forms include suppositories, sprays, ointments, cremes, gels, inhalants, dermal patches, implants, etc.
In one ment, the compound of the present invention is administered in an amount from about 0.001 mg/kg body weight to about 100 mg/kg body weight per day. In par- ticular, daily dosages may be in the range of 0.01 mg/kg body weight to about 50 mg/kg body weight per day. The exact dosages will depend upon the frequency and mode of administra- tion, the sex, the age the weight, and the general condition of the subject to be treated, the nature and the severity of the condition to be d, any concomitant es to be treated, the desired effect of the treatment and other factors known to those d in the art.
A typical oral dosage for adults will be in the range of 0.1-1000 mg/day of a compound of the present invention, such as 1-500 mg/day, such as 1-100 mg/day or 1-50 mg/day.
Conveniently, the compounds of the ion are administered in a unit dosage form contain- ing said nds in an amount of about 0.1 to 500 mg, such as 10 mg, 50 mg 100 mg, 150 mg, 200 mg or 250 mg of a compound of the present invention.
For parenteral administration, solutions of the compound of the invention in sterile s solution, aqueous propylene glycol, aqueous vitamin E or sesame or peanut oil may be employed. Such aqueous solutions should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or e. The aqueous solutions are par- ticularly suitable for intravenous, intramuscular, aneous and eritoneal administra- tion. The sterile aqueous media employed are all readily available by standard techniques known to those skilled in the art.
Suitable pharmaceutical carriers include inert solid diluents or fillers, sterile aqueous solution and s organic solvents. Examples of solid carriers are lactose, terra alba, su- crose, cyclodextrin, talc, gelatine, agar, pectin, acacia, magnesium stearate, stearic acid and lower alkyl ethers of cellulose. Examples of liquid carriers are syrup, peanut oil, olive oil, phospho lipids, fatty acids, fatty acid amines, yethylene and water. The pharmaceutical compositions formed by combining the compound of the invention and the pharmaceutical acceptable carriers are then readily stered in a y of dosage forms suitable for the disclosed routes of administration.
Formulations of the t invention suitable for oral administration may be pre- sented as discrete units such as capsules or tablets, each containing a predetermined [Annotation] sak amount of the active ingredient, and which may e a suitable excipient. Furthermore, the orally available formulations may be in the form of a powder or granules, a solution or sus- pension in an s or ueous liquid, or an oil-in-water or water-in-oil liquid emulsion.
If a solid carrier is used for oral administration, the preparation may be tablet, e.g. placed in a hard gelatine capsule in powder or pellet form or in the form of a troche or loz- enge. The amount of solid carrier may vary but will usually be from about 25 mg to about 1 g.
If a liquid carrier is used, the preparation may be in the form of a syrup, emulsion, soft gela- tine capsule or sterile injectable liquid such as an aqueous or non-aqueous liquid suspension or solution.
Tablets may be prepared by mixing the active ingredient with ordinary adjuvants and/or diluents followed by the compression of the mixture in a conventional tabletting ma- chine. Examples of nts or diluents comprise: Corn starch, potato starch, talcum, mag- nesium stearate, gelatine, lactose, gums, and the like. Any other adjuvants or additives usu- ally used for such purposes such as colourings, flavourings, preservatives etc. may be used provided that they are compatible with the active ingredients.
All references, ing publications, patent applications, and patents, cited herein are hereby incorporated by reference in their entirety and to the same extent as if each refer- ence were individually and specifically indicated to be incorporated by reference and were set forth in its entirety herein (to the maximum extent permitted by law), regardless of any sepa- rately provided incorporation of particular nts made elsewhere herein.
The use of the terms “a” and “an” and “the” and similar referents in the context of de- scribing the invention are to be construed to cover both the ar and the , unless otherwise indicated herein or clearly contradicted by context. For example, the phrase "the compound" is to be understood as referring to s "compounds" of the invention or par- ticular described aspect, unless othenNise indicated.
The description herein of any aspect or aspect of the invention using terms such as “comprising”, “having,” “including,” or “containing” with reference to an element or elements is intended to provide support for a similar aspect or aspect of the ion that “consists of”, “consists essentially of”, or “substantially comprises” that particular element or elements, unless ise stated or clearly contradicted by context (e.g., a composition described herein as comprising a particular element should be understood as also describing a compo- sition consisting of that element, unless otherwise stated or clearly contradicted by context).
It should be understood that the various aspects, embodiments, implementations and es of the invention ned herein may be claimed tely, or in any combination.
[Annotation] sak The compounds of formula I may be prepared by methods described below, together with synthetic methods known in the art of organic chemistry, or modifications that are familiar to those of ordinary skill in the art. The starting materials used herein are available commer- cially or may be prepared by routine methods known in the art, such as those method de- d in rd reference books such as “Compendium of Organic Synthetic Methods, Vol. l-Xll” shed with Wiley-lnterscience). Preferred methods include, but are not limited to, those described below.
The schemes are representative of methods useful in synthesizing the compounds of the present invention. They are not to constrain the scope of the invention in any way.
Methods of Preparation of the Compounds of the Invention.
The nds of the invention with formula I can be ed from intermediate Ill and II as described in Scheme 1.
Scheme 1 If X is a hydroxyl, the carboxylic acid II and the amine Hi can be condensed to form the amide I using standard peptide coupling chemistry, e.g. as described in the textbook Syn- thetic Peptides A user’s Guide (Edited by Gregory A. Grant, W H. Freeman and company (1992) ISBN 07009-1) or as described in the ok Houben-Wey/ Volume E22a Synthesis of es (George Thiemes Ver/ag Stuttgart (2003) 4th ed.). One example of this amide formation is the use of the coupling t HATU (O-(benzotriazolyl)-N,N,N’,N’- ethyluronium hexafluorophosphate). Typically, one eq. of II is reacted with one eq. of HATU in the presence of two eq. of a tertiary amine e.g. triethylamine in a suitable t e.g. DMF. After a short period of time (e.g. five minutes) this mixture is reacted with one eq. of III to form |. Another example of this amide formation uses 1-hydroxybenzotriazole together with the water soluble carbodiimide EDC (CAS 259528) and triethyl amine in a suitable solvent e.g. THF. These reactions are usually performed at room ature or between 0 0C and 50 0C.
[Annotation] sak If X is a chloride (e.g. prepared from the carboxylic acid ll, X = OH, using thionyl chloride) lll can be reacted with II to form | in the presence of a tertiary amine in a suitable solvent. Alter- natively, the carboxylic acid chloride (ll, X = Cl) can be d with N-hydroxy succinimide to produce the HOSU ester which can be isolated and then reacted with I” to e |.
Methods of Preparation of the Intermediates of the Invention.
The Intermediates of the invention with formula II are either commercially available or can be prepared as described in Scheme 2.
R4 R4 R3 R5 R3 R5 R2 0 R2 0 R1 R1 K racemic-trans-ll ethylester R3 R5 SFC separation R2 OH racemic trans || " Scheme 2. Preparation of the (1S, 2S) enantiomer of a II.
Ethyldiazoacetate can be d with the styrene in Scheme II to produce the race- ans ll ethyl ester. This ester can then be hydrolyzed to racemic trans II which can then be separated into the two enantiomers using SFC. Alternatively, racemic trans II can be re- solved into the two enantiomers by known methods as described in the textbook “Enanti- omers, Racemates and Resolutions” (J. Jaques, et al., John Wiley and sons, New York (1981)). r preparation of the compounds with formula II is described in Scheme 3. This method has been described in detail in W02012/O37258 [Annotation] sak R4 R4 R3 R5 OEt R3 R5 EtO\ I OtBU —S— NaH LiOH + PW I H —» H / OtBu —> —’ R2 0 O Toluene R2 NaOtBu R1 0 R1 0 DMSO R3 R5 R2 OH Separation racemic trans ll " Scheme 3. Preparation of the (1S, ZS) enantiomer of a II.
The benzaldehyde shown in Scheme 3 can be reacted with the anion of (Diethoxy- phosphoryl)—acetic acid tert-butyl ester to produce the unsaturated ester shown. Cylopropana- tion followed by hydrolysis then produces Racemic trans II, which can be ted as de- scribed above.
The lntermediates of the invention with formula III are either commercially available or can be prepared as described in Scheme 4 in which R5 is CHZOH.
Br A“ / is9 0 H OSITBS ' 0 R10 S\NH + O _> g —> / / 2 OSiTBS tBU \N BuLi OSiTBS OSITBS OH i '/ i s A s A A t /Bu \N 7¢ + / As Bu \N 7¢ 7? I A I As H2N As H H A _. I / /A 9 / 9 9 R11 R11 R11 R10 R10 R10 Scheme 4. ation of the chiral amines of formula III, with R5 = CHZOH. The method is described in: Barrow, J. C. et al. Tetrahedron Letters (2001) 2051.
[Annotation] sak (R)—(+)methylpropanesulfinamide can be reacted with (tertbutyldimethylsilyloxy ldehyde as bed in the literature (Barrow, J. C. et al. Tetrahe- dron Letters (2001) 2051) to e the sulfinimine shown in Scheme 4. 1,2- addition of an organometallic (e.g. a Grignard reagent or an aryllithiumreagent (shown in Scheme 4) re- agent to this sulfinyl imines then gives the two diastereomeric protected amino alcohols shown in scheme 4. These isomers can be separated e.g. by silica gel chromatography and the protecting groups are then removed under acidic conditions.
Another method using enantiopure tert-butanesulfinamide is shown in Scheme 5 (Robak, M., Herbage, M., Ellman, Chem. Rev. 2010, 110, 3600-3740 and references cited herein). For simplicity, the method is only rated for R5 = CH3, but the method is not lim- ited to R5 = CH3.
I I8 ion X8\ + _, —> NHz 9 0 o t t /l A \N 7\\ + A /l A B” As B” \N 7C 7C As HZN As H —» I H I I /l 9 /l /l 9 9 R11 R11 R11 R10 R10 R10 Minor isomer Major isomer Scheme 5. Preparation of the chiral amines of formula III, with R5 = CH3. The method is de- scribed in: Robak, M., Herbage, M., Ellman, Chem. Rev. 2010, 110, 3600-3740 and refer- ences cited herein.
(R)—(+)methylpropanesulfinamide can be reacted with a suitable ketone and titanium (lV)ethoxide in a suitable solvent e.g. THF under heating conditions to produce the sulfinyl imine shown in scheme 5. This imine can be d, with some selectivity using a reducing agent (e.g. L-selectride) in a suitable solvent (e.g. THF) at a suitable ature (e.g. -70 0C) to produce the major and the minor isomer shown in Scheme 5. The major iso- mer can be isolated by e.g. silica gel chromatography and the chiral auxiliary can then be re- moved with acid (e.g. HCI in water to produce lll).
EXAMPLES [Annotation] sak The ion will be illustrated by the following non-limiting examples.
Abbreviations AcOH = acetic acid. OLD = specific optical rotation. Aq = Aqueous. BBr3 = boron tribromide (used as DCM solution; Aldrich 17,893-4). Boc20 = Boc anhydride / di-t—butyl dicarbonate (e.g. Aldrich 19,913-3). Brine = saturated aqueous on of sodium chloride. CDCI3 deuter- ated chloroform (e.g. Aldrich 225789). Celite = filter-aid. CH3| = methyl iodide / iodomethane (e.g. Aldrich 28,956-6). Cs2C03 = cesium carbonate (Aldrich 441902). DCM = dichloro- e. DMF = yl formamide. DMSO = dimethyl sulfoxide. ds-DMSO = deutorated yl ide (e.g. Aldrich 296147). ELSD = evaporative light scattering detection. Et3N = triethyl amine. EtOAc = ethyl acetate. 99% EtOH = te ethanol. EtZO = diethyl ether. h = hours. HATU = O-(7-Azabenzotriazoly|)-N,N,N’,N’-tetramethyluronium hexaflouruphos- phate. HBTU = 2-(1H-Benzotriazolyl)—1,1,3,3-tetramethyluronium hexaflourophosphate. i= iso. K2C03 = ium carbonate (e.g. Aldrich 20,961-9). LDA = lithium ropylamide (used as a THF/heptane/ethylbenzene solution; Fluka 62491 ). LC/MS = high-performance liquid chromatography / mass ometer. LAH = lithium aluminium hydride (used as a 1M THF solution; Aldrich 21,277-6). MeOH = methanol. min = minutes. NaCNBH3 = sodium cyanoborohydride (Aldrich 15,615-9). NaH = sodium hydride (used as a 60% dispersion; Aldrich 45,291-2). NaOH = gueous solution of sodium hydroxide. Pd/C = palladium-on- charcoal (e.g. Aldrich 20,569-9). PTSA = para-toluene sulfonic acid hydrate (e.g. Aldrich —5). rt = room temperature. RT = retention time. sat. NaHCOs = saturated s so- lution of sodium hydrogen carbonate. sat. NH4C| = saturated aqueous solution of ammonium chloride. SFC = supercritical flash chromatography. TFA = trifluoroacetic acid. THF = tetrahy- drofuran (dried over 4A molecular sieves). TLC = thin layer chromatography.
Chemical names were obtained using the software MDL RAW 2.5 from MDL informa- tion systems Spectroscopic methods.
Method A: LC-MS were run on a Sciex AP|150EX equipped with APPl-source operating in positive ion mode. The HPLC consisted of Shimadzu LC10-ADvp LC pumps, SPD-M20A PDA detector (operating at 254 nm) and SCL-10A system controller. Autosampler was Gilson 215, Column oven was a Jones Chromatography 7990R and ELS detector was a Sedere Sedex 85.
[Annotation] sak LC-conditions: The column was a Waters Symmetry C-18, 4.6x30 mm, 3.5 pm operating at 60°C with 3.0 mL/min of a binary gradient consisting of water + 0.05% TFA (A) and methanol + 0.05% TFA (B).
Gradient: 0.01 min 17% B 0.27 min 28% B 0.53 min 39% B 0.80 min 50% B 1.07 min 59% B 1.34 min 68% B 1.60 min 78% B 1.87 min 86% B 2.14 min 93% B 2.38 min 100% B 2.40 min 17% B 2.80 min 17% B Total run time: 2.8 min.
The retention times (tR) are expressed in minutes based on UV-trace at 254 nm.
Method B: LC-MS were run on Waters Acquity UPLC-MS consisting of Waters Acquity including column mamager, binary solvent manager, sample organizer, PDA or (operating at 254 nM), ELS detector, and SQD-MS ed with APPl-source operating in positive ion mode.
LC-conditions: The column was Acquity UPLC BEH C18 1.7um ; 2.1x50mm ing at 60°C with 1.2 ml/min of a binary gradient consisting of water + 0.05 % trifluoroacetic acid (A) and acetonitrile + % water + 0.035 % trifluoroacetic B) Gradient: 0.00 min 10% B 1.00 min 100% B 1.01 min 10% B 1.15 min 10% B Total run time: 1.2 min.
The retention times (tR) are expressed in minutes based on UV-trace at 254 nm.
[Annotation] sak Method C: Preparative supercritical fluid chromatography (SFC) was performed on a Berger ram ll operating at 50 mL/min at 35°C and 100 bar backpressure using stacked injections. The col- umn was a pakAD 5 u, 250x21 mm. The eluent was C02 (70%) and ethanol (30%).
Method D: Preparative supercritical fluid chromatography (SFC) was performed on a Thar SFC-80 oper- ating at 60 g/min at 35°C and 140 bar backpressure using stacked injections. The column was a ChiralPakAD-H (250x30 mm). The eluent was C02 (88%) and Ethanol (12%).
Method E: Preparative supercritical fluid chromatography (SFC) was performed on a Thar SFC-200 op- erating at 100 g/min at 35°C and 140 bar backpressure using stacked injections. The column was a ChiralPakAD-H (250x30 mm). The eluent was C02 (90%) and Ethanol (10%).
Method F: omeric excess (ee) was determined on an Aurora Fusion A5 SFC system operating at 3 ml/min at 40 °C and 100 bar backpressure. The column was a Chiralpak AD (150x4.6 mm).
The eluent was C02 (70%) and ethanol + 0.1% diethyl amine (30%). 1H NMR spectra were recorded at 500.13 MHz on a Bruker Avance DRX-500 ment at T=303.3 K or at 600 MHz on a Bruker Avance AV-lll-600 instrument. Chemical shift values are expressed in ppm-values relative to tetramethylsilane unless noted otherwise. The follow- ing abbreviations or their combinations are used for multiplicity of NMR signals: s = singlet, d = t, m = let and br = broad.
Preparation of intermediates Pre aration of bromo s.
IM1 : 5-Bromo-2—isopropoxy—pyridine BraI k\ 60% NaH in 0“ (15:1, Sodium hydride:Minera| Oil, 5.20 g) was added in two ns to iso- propyl alcohol (150 mL) at room temperature under N2. The mixture was stirred at 60°C for 30 min. 5-bromochloropyridine (10.00 g, 51.96 mmol) was added in two portions and the mix- ture was stirred at reflux 4h and then at 80°C overnight. The solution was concentrated in [Annotation] sak vacuo. Water (50 mL) and EtOAc (50 mL) was added and the layers were separated. The aqueous layer was extracted with EtOAc (50 mL). The combined organic layers were washed with brine, dried over MgSO4, filtered and the solvent was removed in vacuo. The crude prod- uct was subjected to flash chromatography a, 0-50% EtOAc in heptanes) to give the title compound as a clear oil (8.74 g, 78%). 1H NMR (600 MHz, DMSO) 6 8.17 (s, 1H), 7.61 (dd, 1H), 6.59 (d, 1H), 5.23 (m, 1H), 1.33 (s, 6H).
IM2: 5-Bromo(2, 2, 2-trifluoro-ethoxy)-pyridine Prepared analogously to IM1 to give the title compound as a ess liquid (2.78 g, 54%) iently pure for the next step.
IM3: 5-Bromopropoxy—pyridine Potassium tert—butoxide (1.85 g, 16.5 mmol) was added to a mixture of 5-bromo chloropyridine (2.89 g, 15.0 mmol) and 1-propanol (1.230 mL, 16.5 mmol) in THF (15 mL).
The reaction mixture was heated at 120°C for 30 minutes in a microwave reactor. The mixture was poured into a mixture of water (50 mL) and EtOAc (100 mL). The organic layer was washed with brine, dried over MgSO4 and evaporated to s. Flash chromatography (sil- ica, 0-20% EtOAc in heptanes) gave the title compound as a yellow oil (3.13 g, 97%) suffi- ciently pure for the next step.
IM4: 5-Bromo(2, 2, 2-d3)-ethoxy-pyridine [Annotation] sak Prepared analogously to IM3 using commercially available 2,2,2-d3-ethanol -Aldrich, catalog no 329347) to give the title compound as a colorless oil (2.53 g, 82%) sufficiently pure for the next step.
IM5: 5-Bromo-2—(1 , 1, 2, 2, 2-d5)-ethoxy-pyridine Prepared ously to IM3 using commercially available 1,1,2,2,2-d5-ethanol - Aldrich, g no 489336) to give the title compound as a colorless oil (1.16 g, 87%) suffi- ciently pure for the next step.
IM6: 5-Bromo-2—(1 1 -d2)-ethoxy-pyridine Prepared analogously to IM3 using commercially available 1,1-d2-ethanol (Sigma-Aldrich, catalog no ) to give the title compound as a colorless oil (2.61 g, 85%) sufficiently pure for the next step.
IM7: 5-Bromo-2—(2—methoxy—ethoxy)-pyridine 2-Methoxyethanol (5.12 mL, 65.0 mmol) was dissolved in 1,4-dioxane (125 mL). Potassium tert—butoxide (7.00 g, 62.4 mmol) was added under N2. The mixture was stirred for 10 min- utes. 5-Bromochloropyridine (10.0 g, 52.0 mmol) was added and the resulting mixture was refluxed for 2 hours. The mixture was poured into brine and extracted with EtOAc. The or- ganic layer was washed with brine, dried over MgSO4 and evaporated to s. Purification by flash chromatography (silica, heptanes/EtOAc 4:1) gave the title compound as a colorless oil (8.74 g, 73%) sufficiently pure for the next step.
IM8: 5-Bromomethoxymethyl-pyridine [Annotation] sak \' 0\ To a solution of 5-bromopyridinecarbaldehyde (5.00 g, 26.9 mmol) dissolved in a e of ethanol (75 mL) and THF (25 mL) was added sodium borohydride (0.407 g, 10.8 mmol) in small portions. After 45 minutes 0.5 mL water was added and the mixture and evaporated to dryness. The oily residue was subjected to flash chromatography (silica, EtOAc/EtOH/Et3N 90:5:5) to give (5-bromo-pyridinyl)—methanol (4.81 g, 86%) as pale-yellow oil.
A solution of this (5-bromo-pyridinyl)—methanol (4.80 g, 23.0 mmol) in DMF (25 mL) was added drop wise over 5 minutes to a slurry of sodium hydride (1.10 g, 27.6 mmol) in DMF (50 mL) at 0 °C under N2. The mixture was stirred for 15 s before the drop wise addition of a solution of methyl iodide (1.57 mL, 25.3 mmol) in DMF (25 mL). The mixture was allowed to reach room temperature and was then stirred overnight. The mixture was poured into brine and extracted with EtOAc. The combined organic layers were thoroughly washed with brine, dried over MgSO4 and ated to dryness to give the title compound as a yellow oil (4.77 g, 98%) sufficiently pure for the next step.
IM9: 5-Bromocyc/obutoxy—pyridine Prepared analogously to IM3 using commercially available cyclobutanol to give the title nd as a clear oil (2.72 g, 80%) sufficiently pure for the next step.
IM1 0: 5-Bromo[(R)-(tetrahydro-furanyl)oxy]—pyridine To a solution of 5-bromochloropyridine (10 g, 52.1 mmol) in 100 mL of DMF was added commercially ble (R)—(-)—3-hydroxytetrahydrofuran (6.87 g, 78.1 mmol) and Cs2C03 (33.85 g, 0.104 mol), the resulting mixture was heated 90 °C for 36 hours. The solvent was concentrated and the residue was extracted with EtOAc ), washed with water (200ml).
The organic layer was dried over NaZSO4, concentrated and purified by chromatography on silica gel (eluting with Petrol ether: EtOAc = 100:1) to afford o[(R)-(tetrahydro-furan- 3-yl)oxy]—pyridine (5.9 g, yield: 47%) as a solid. 1HNMR (CDCI3 400MHz): 68.15 (d, J = 2.4 Hz, 1 H), 7.61-7.64 (m, 1 H), 6.64 (d, J: 8.8 Hz, 1 H), 5.47-5.50 (m, 1 H), 3.85-4.02 (m, 4 H), 2.07-2.28 (m, 2 H). [a1D20 = +18.5 (C=0.189, CHCI3).
[Annotation] sak IM1 1 : 5-Bromo-2—[(S)-(tetrahydro-furanyl)ony-pyridine Bra 0 \ u' Prepared ously to IM10 using commercially available (S)-(+)hydroxytetrahydrofuran to afford 5-Bromo[(S)-(tetrahydro-furany/)oxy]-pyridine (9.62 g, yield: 51%) as a solid. 1HNMR (CDCI3400MHz): 68.16 (d, J = 2.4 Hz, 1 H), 7.62- 7.65 (m, 1 H), 6.64-6.66 (m, 1 H), 5.48-5.52 (m, 1 H), 3.99-4.03 (m, 2 H), 3.86-3.97 (m, 2 H), 2.20-2.29 (m, 1 H), 2.08-2.15(m, 1 H). [011020 = -20.7 (c=0.21, .
IM12: 5-Bromo(tetrahydro-pyranyloxy)-pyridine Prepared analogously to IM3 using commercially available tetrahydropyranol to give the title compound sufficiently pure for the next step.
IM1 3: 6-Bromo-[1, 3]dioxolo[4, 5-b]pyridine Br Br / IN / + N Br/\Br I \ \ OH 0 OH 0J To a suspension of o-pyridine-2,3-diol (10.0 g, 52.63 mmol, commercially available, CAS 34206-49—0) in NMP (100 mL) was added K2C03 (21.97 g, 158 mmol) and dibromo methane (10.97 g, 63.16 mmol). The reaction mixture was heated to 90 °C for 16 h. EtOAc was added and the salts were filtered off. Water was added, the phases were separated and the aq layer was extracted with more ethyl acetate. The combined organic layers was dried over ous NaZSO4 and concentrated under vacuo to get the crude compound. The crude compound was purified by silica gel chromatography (eluent 5 % ethyl acetate in petrol ether). Yield of 6-Bromo-[1,3]dioxolo[4,5-b]pyridine 2.2 g (21%) pure by 1H NMR (400 MHz, DMSO) 6 7.71 (d, 1H, J = 2 Hz), 7.55 (d, 1H, J = 2 Hz), 6.20 (s, 2H). Mp 69-71 C.
IM1 4: 7-Bromo-2, 3-dihydro-[1, 4]dioxino[2, 3-b]pyridine [Annotation] sak Br Br / IN + Br/W / ; IN \ \ OH 3, 0 To a sion of 5-Bromo-pyridine-2,3-diol (10.0 g, 52.63 mmol, commercially available, CAS 34206-49—0) in DMF (150 mL) was added K2C03 (21.78 g, 158 mmol) and 1,2-dibromo ethane (11.87 g, 63.2 mmol). The reaction e was heated to 100 °C for 5 h. The reaction mixture was cooled to rt and poured into ice cold water EtOAc was added and the phases were separated and the aq layer was extracted with more ethyl acetate. The combined or- ganic layers was dried over anhydrous NaZSO4 and concentrated under vacuo to get the crude compound. The crude compound was purified by silica gel chromatography (eluent 10 % ethyl acetate in petrol ether). Yield of 6-Bromo-[1,3]dioxolo[4,5-b]pyridine 2.2 g (18 %) pure by 1H NMR (400 MHz, DMSO) 6 7.85 (d, 1H, J = 2 Hz), 7.59 (d, 1H, J = 2 Hz), 4.41 (m, 2H), 4.27 (m, 2H).
Acetylation of pyridines.
IM15: 1-(6-Ch/oro-pyridin-3—y/)-ethanone A round ed flask was charged with ochloropyridine (5.30 g, 27.6 mmol) in THF under N2 and cooled at 0°C. A solution of 1 M iso-propylmagnesiumchloride - lithium chloride complex in THF (40 mL) was added drop wise over 15 min. After 70 min N-methoxy- N-methylacetamide (4.1 mL, 38 mmol) was added drop wise. After stirring for 5 min at 0 °C the cooling bath was removed. The mixture was left stirring overnight and was then quenched by the addition of 100 mL saturated aqueous NH4CI solution. The mixture was ex- d with 3x100 mL EtOAc. The combined organic layers were washed with water followed by brine and dried over MgSO4. Evaporation of the volatiles at 80 °C, 10 mbar for 1h gave the title compound (3.596 g, 84) sufficiently pure for the next step.
IM16: 1 -(6-Iso-propoxy—pyridinyI)-ethanone [Annotation] sak A round-bottomed flask was d with 5-bromoiso-propoxypyridine (IM1) (5.00 g, 23.1 mmol) in THF (100) under N2 and cooled in an acetone/dry-ice bath to -66 °C (internal temperature ). A solution of2.5 M n-butyllithium in hexane (10.1 mL, 25.3 mmol) was added drop wise over 10 minutes at keeping the internal temperature below -55 °C. The mixture was stirred at -65 °C for 15 minutes. N-methoxy-N-methylacetamide (3.07 mL, 28.9 mmol) dis- solved in THF (10 mL) was then added drop wise over 10 minutes while keeping the internal temperature below -65 °C. After ng for 1 h the mixture was allowed to reach room tem- perature. The mixture was poured into saturated aqueous NH4CI on and extracted with EtOAc. The combined organic layers were washed with brine, dried over MgSO4 and evapo- rated to dryness. Flash chromatography a, heptanes/EtOAc 4:1) gave the title compound as a colorless oil (3.20 g, 77%) sufficiently pure for the next step.
IM17: 1 -(6-Methoxymethyl-pyridinyl)-ethanone \ 0\ Prepared analogously to IM16 from IM8 to give the title compound as a colorless liquid (0.379 g, 17%) iently pure for the next step.
IM1 8: 1-[6-(2, 2, 2— Trifluoro-ethoxy)-pyridinyl]—ethanone \ F O/\F'< Prepared analogously to IM16 from IM2 to give the title compound as a colorless liquid (1.234 g, 48%) sufficiently pure for the next step.
IM1 9: 1 -[6-(2-Methoxy—ethoxy)-pyridinyl]—ethanone Prepared analogously to IM16 from IM7 to give the title compound as a colorless liquid (2.13 g, 57%) sufficiently pure for the next step.
IM20: 1-(2-Ethoxy-pyridinyl)-ethanone [Annotation] sak Prepared analogously to IM16 from cially available 4-bromoethoxy-pyridine, Synchem OHG catalog no CT091 to give the title compound as a colorless liquid (1.20 g, 49%) sufficiently pure for the next step.
IM21 : 1-[1, 3]Dioxolo[4, ridiny/—ethanone A round-bottomed flask was d with 6-Bromo-[1,3]dioxolo[4,5-b]pyridine IM13 (1.74 g, 8.61 mmol) in DMF (25 ml) under N2 and tributyl(1-ethoxyvinyl)tin (3.65 ml, 10.8 mmol) was added. Tetrakistriphenylphosphinepalladium(0) (0.50 g, 0.43 mmol) was added and the solu- tion was stirred at 65 °C overnight. The mixture was added to water and EtOAc and the phases were separated. The org phase was washed with brine, dried (MgSO4) filtered and was rotovaped. The e was dissolved in THF (100 ml), and a mixture of water (15 ml) and conc. HCI (2.5 ml) was added and the solution was stirred at rt 5 min. The solution was added to brine and sat NaHC03 solution was added until the solution was slightly alkaline.
The org Phase was extracted with EtOAc and the phases were separated. The org phase was washed with brine, dried (MgSO4) filtered and was rotovaped. The residue was redis- solved in THF (10 ml) EtOAc (20 ml) and heptanes (20 ml). The mixture was concentrated until 25 ml remained and cooled in ice. A solid precipitated and was collected by filtration.
Yield: 0.942 g (66%) of IM21. 1H-NMR (500 MHz, DMSO) 6 8.47 (s,1H), 8.54 (s, 1H), 6.27 (s, 2H), 2.53 (s, 3H).
IM22: 1 -(2, 3-Dihydro-[1 , 4]dioxino[2, 3—b]pyridiny/)-ethanone [Annotation] sak Prepared analogously to IM21 from with o-2,3-dihydro-[1,4]dioxino[2,3-b]pyridine (3.00 g, 13.9 mmol) to give the title compound as a white powder (1.84 g, 74%). 1H-NMR (500 MHz, DMSO) 6 8.47 (s, 1H), 8.54 (s, 1H), 6.27 (s, 2H), 2.53 (s, 3H).
IM23: 1-(6-Ethyl—pyridin-3—yl)-ethanone A dry round bottomed flask was charged with 1-(6-chloropyridinyl)—1-ethanone (IM15) (3.596 g, 23.11 mmol) and [1 s(dipheny|phosphino)ferrocene]-dichloropalladium(ll) (1.694 g, 2.315 mmol) in THF (100 mL) under N2. A1 M solution of diethyl zinc in hexane (35 mL, 35 mmol) was added drop wise to this mixture followed by N,N-dimethylaminoethanol (0.50 mL, 5.0 mmol). The mixture was heated to reflux for 30 minutes. The mixture was cooled to room temperature and then quenched by the addition of ted aqueous NH4C| solution (100 mL). The mixture was filtered through a plug of celite. The aqueous layer was extracted with EtOAc (3x100 mL). The combined organic layers were washed with water, brine and then dried over Mg2804. Flash Chromatography (120 g silica, 0-40% EtOAc in hep- tanes) gave the title compound as a yellow oil (0.699 g, 20%) sufficiently pure for the next step.
Preparation of chiral amines.
Chiral amines were made, if not commercially available, according to well-described proce- dure for either 1,2-stereoselective reduction of sulfinyl imines or 1,2-stereoseelctive addition of organometallic reagents to sulfinyl imines. These s have been described by Chel- lucci, G., Baldino, 8., Chessa, 8., Pinna, G., Soccolini, 8., Tetrahedron Asymmetry 2006, 17, 3163-3169, Evans, J., Ellman, J., J. Org. Chem. 2003, 68, 9948-9957 and Robak, M., Herb- age, M., Ellman, J., Chem. Rev. 2010, 110, 3600-3740 and nces cited herein.
IM24: (8)(6-Isopropoxy—pyridin-3—yI)-ethy/amine [>le mI k\ Ste 1: ion of sulfin l imine: 1-(6-Iso-propoxy-pyridinyl)-ethanone IM16 (3.20 g, 17.8 mmol) was dissolved in THF (55 mL) under N2. R(+)methylpropanesulfinamide (2.21 g, 18.2 mmol) and tita- [Annotation] sak nium(|V)ethoxide (7.40 mL, 35.7 mmol) was added. The mixture was refluxed for 24 hours.
The mixture was allowed to reach room temperature. The mixture was diluted with EtOAc (200 mL) and poured into ice/brine. The ing slurry was filtered through Celite. The or- ganic layer was washed with brine, dried over M9804 and evaporated to dryness. Flash chromatography (silica, heptanes/EtOAc 2:1) gave (R)—2-methyl-propanesulfinic acid [1-(6- isopropoxy-pyridinyl)-ethy|idene]-amide (4.04 g, 80%) as a yellow oil sufficiently pure for the next step.
Step 2: Reduction of the imine: A round-bottomed flask was charged with (R)—2-methyl-propanesulfinic acid [1-(6-iso— propoxy-pyridinyl)—ethylidene]—amide (4.00 g, 14.2 mmol) in THF (50 mL) under N2 and cooled to -66°C (internal temperature). A 1.00 M solution of L-Selectride in THF (29.0 mL, 29.0 mmol) was added drop wise over 15 minutes. The mixture was d at -70°C for 1 hour. The cold mixture was poured into saturated aqueous NH4CI solution. The mixture was extracted with EtOAc. The combined organic layers were washed with brine, dried over M9804 and ated to dryness. Flash chromatography (silica, EtOAc) gave (R)—2-methyl- propanesulfinic acid [(S)—1-(6-isopropoxy-pyridinyl)—ethyl]—amid (2.91 g, 72%) as a color- less powder. Diastereomeric excess >95% based on 1H NMR.
Step 3: Removal of chiral auxiliaLy: (R)—2-Methyl-propanesulfinic acid -(6-isopropoxy-pyridinyl)—ethyl]—amide (2.90 g, 10.2 mmol) was dissolved in methanol (48 mL). A mixture of 12.0 M HCI in water (4.25 mL) and water (4.25 mL) was added drop wise over 3 minutes. The mixture was stirred at room temperature overnight. The mixture was ated to dryness. The oily e was sub- jected to flash chromatography a, EtOAc/EtOH/triethylamine 90:5:5) on a short column to give (S)—1-(6-isopropoxy-pyridinyl)—ethylamine IM24 (1.71 g, 93%) as a pale-yellow oil suf- ficiently pure for the next step. The overall yield from 1-(6-isopropoxy-pyridinyl)—ethanone IM16 was 54%.
IM25: (6-Ethyl—pyridinyl)-ethylamine Prepared analogously to IM24 from IM23 to give the title compound sufficiently pure for the next step.
IM26: (S)(6-Methoxymethyl-pyridinyI)-ethy/amine [Annotation] sak Prepared analogously to IM24 to give the title compound iently pure for the next step.
IM27: (S)-1 -[6-(2, 2, 2- Trifluoro-ethoxy)-pyridinyl]—ethy/amine Prepared analogously to IM24 to give the title compound sufficiently pure for the next step.
IM28: (S)[6-(2-Methoxy-ethoxy)-pyridiny/]-ethylamine Q‘Hz \ O/\/O\ Prepared analogously to IM24 to give the title compound sufficiently pure for the next step.
IM29: (2-Ethoxy—pyridinyl)-ethy/amine E‘Hz Prepared analogously to IM24 to give the title compound sufficiently pure for the next step.
IM30: (S){6-[(S)-(Tetrahydro-furany/)oxy]-pyridiny/}-ethylamine /N O \I L) Prepared analogously to IM24 to give the title compound sufficiently pure for the next step.
IM31 : (S){6-[(R)—(Tetrahydro-furanyl)oxy]-pyridinyl}-ethy/amine [Annotation] sak Prepared analogously to IM24 to give the title compound sufficiently pure for the next step.
IM32: (S)[1, 3]Dioxolo[4, 5-b]pyridiny/-ethylamine ed analogously to IM24 to give the title compound sufficiently pure for the next step.
IM33: (S)(2, dro-[1,4]dioxino[2, 3-b]pyridin- 7-yl)-ethy/amine Prepared analogously to IM24 to give the title compound sufficiently pure for the next step.
IM34: (S)(2-Ethoxy-pyrimidinyl)-ethy/amine ed analogously to IM24 to give the title compound sufficiently pure for the next step.
IM35: 2—[5-((S)Amino-ethyl)-pyridin-2—yloxy]-ethanol E‘Hz o3 JOH|\ 0 Prepared analogously to IM24 to give the title compound sufficiently pure for the next step.
[Annotation] sak IM36: (R)Amino(6-propoxy—pyridiny/)-ethanol Br 1 tB) u'L' )(S‘gH / N +><S N ' I 2)Separate Hm THL HOHO A 1.7 M solution of tert—butyllithium in pentane (15.2 mL, 25.8 mmol) was added drop wise to a stirring solution of 5-bromopropoxypyridine IM3 (2.54 g, 11.8 mmol) ved in dry THF (29.4 mL) at -78°C under Ar. The solution was subsequently stirred at this temperature for 30 min. A solution of (R)—2-methyl-propanesulfinic acid [2-(tert-butyl-dimethyl-silanyloxy)— ethylidene]—amide IM49 (3.26 g, 11.8 mmol) in dry THF (15 mL) was then added drop wise at -78°C and the solution was stirred at this temperature for 30 min. The cooling bath was re- moved and the mixture was allowed to reach room temperature overnight. The mixture was quenched with saturated s NH4CI solution (75 mL) and EtOAc (150 mL). The phases were separated and the organic layer was washed with brine and then dried over MgSO4.
Flash chromatography (silica, 10-100% EtOAc in heptanes) gave (R)—2-Methyl-propane sulfinic acid [(R)—2-(tert-butyl-dimethyl-silanyloxy)—1-(6-propoxy-pyridinyl)—ethy|]-amide, the fastest eluding isomer, as a clear oil (2.33 g, 48%) sufficiently pure for the next step. Di- astereomeric excess >95% based on 1H NMR.
A 2.00 M solution of en chloride in diethyl ether (28 mL, 56 mmol) was added to a stirred solution of [(R)—2-(tert-butyl-dimethyl-silanyloxy)—1-(6-propoxy-pyridinyl)-ethy|]-amide (2.33 g, 5.62 mmol) ved in MeOH (11 mL) at 0°C under Ar. After the addition was com- pleted the cooling bath was removed and the solution was stirred at room temperature over- night. The e was then evaporated to dryness and the residue was suspended in methylene chloride and transferred to a short silica gel column. After eluding with EtOAc:EtOH:Et3N (90:5:5) (R)—2-Amino(6-propoxy-pyridinyl)-ethano|, IM36, was ob- tained as an oil (0.813 g, 74%). The l yield from 5-bromopropoxypyridine IM3 was 36%. 1H NMR (600 MHz, CDCI3) 6 8.61 (s, 1H), 8.60 (dd, 1H), 7.71 (d, 1H), 4.22 (t, 2H), 4.06 (m, 1H), 3.72 (m, 1H), 3.60 (m, 1H), 1.78 (m, 2H), 1.24 (m, 1H), 1.02 (m, 4H).
IM37: (R)Amino-2—(6-isopropoxy—pyridin-3—y/)-ethanol ation] sak '2‘“2 OH \ Prepared analogously to IM36 to give the title compound sufficiently pure for the next step (1.07 g, 36% overall yield from IM1).
IM38: (R)Amino(6-ethoxy-pyridinyI)-ethano/ Q‘Hz OH \ Prepared analogously to IM36 to give the title compound sufficiently pure for the next step (0.360 g, 35% overall yield from commercially available 5-bromoethoxy-pyridine, Apollo catalog no OR13065).
IM39: (R)Amino(6-(1, 1, 2, 2, 2-d5)-ethoxy—pyridinyl)-ethanol OH \ D>H\DD D ed analogously to IM36 to give the title compound sufficiently pure for the next step (0.500 g, 22% overall yield from IM5).
IM40: (R)Amino(6-(2, 2, 2-d3)-ethoxy-pyridin-3—y/)-ethanol Q‘Hz Prepared analogously to IM36 to give the title compound iently pure for the next step (0.647 g, 30% overall yield from IM4).
IM41 : (R)Amino(6-(1,1-d2)-ethoxy-pyridiny/)-ethanol [Annotation] sak OH \ Prepared ously to IM36 to give the title compound sufficiently pure for the next step (0.380 g, 18% overall yield from IM6).
IM42: (R)-2—Amino{6-[(S)-(tetrahydro-furanyl)oxy]—pyridinyl}-ethano/ E‘Hz OH \ [2 Prepared analogously to IM36 to give the title compound sufficiently pure for the next step.
IM43: (R)Amino{6-[(R)-(tetrahydro-furany/)oxy]—pyridinyl}-ethano/ E‘Hz OH \ IL) Prepared analogously to IM36 to give the title compound sufficiently pure for the next step.
IM44: (R)Amino-2—(6-cyclobutoxy-pyridiny/)-ethanol m/N D Prepared ously to IM36 to give the title compound sufficiently pure for the next step.
IM45: (R)Amino-2—[6-(tetrahydro-pyranyloxy)-pyridiny/]-ethanol OH \ Prepared analogously to IM36 to give the title compound sufficiently pure for the next step.
Pre aration of carbox lic acids.
[Annotation] sak IM46: (1 2-Pheny/-cyclopropanecarboxy/ic acid (3 O III"V)\OH Commercially available, racemic trans 2-phenyl-cyclopropanecarboxylic acid (Sigma-Aldrich, g no P22354) was subjected to chiral SFC separation, method C to give IM46 as an oil that slowly solidified upon ng. Enantiomeric purity 95% ee (Method F). Specific optical rotation +300.9° [or]D20 (C = 1% EtOH). (Lit: +389° [or]D20 (C = 0.61, CHCI3) Kozikowski et al., J.
Med. Chem. 2009, 52, 1885-1902), (Lit: +311.7° [or]D20 (C = 1.776, EtOH) Walborsky et al., Tetrahedron 1964, 20, 699.) IM47: (1 S,2S)(3-F/uoro-phenyl)-cyc/opropanecarboxylic acid F I""V)kOH Fm Step1 F //\n/ \/ —>O + 3N / O Fm LO Q 0 / OH 0 I""V)kOH Step 1: A round-bottomed flask was charged with 3-fluorostyrene (13.0 g, 0.107 mol) in anhydrous methylene chloride (130 mL). To this mixture was added rhodium acetate dimer (1.30 g, cat amount). A solution of ethyldiazoacetate (33.28 g, 0.291 mol) in anhydrous methylene chlo- ride (130 mL) was added to the reaction via a syringe pump over 5 h and stirred at room tem- perature for 1h in darkness. The reaction mixture was filtered through a plug of , which was washed with water ed by brine. The c layer was dried over NaZSO4 and evaporated to dryness. Flash chromatography (silica, EtOAc/petroleum ether 1:9) gave rac- trans 2-(3-fluoro-phenyl)-cyclopropanecarboxylic acid ethyl ester (13.0 g, 59%) as a colorless liquid sufficiently pure for the next step.
To a solution of rac—trans 2-(3-fluoro-phenyl)-cyclopropanecarboxylic acid ethyl ester (13.0 g, 0.062 mol) in MeOH (310 mL) was added a solution of KOH (35.0 g, 0.625 mol) in MeOH [Annotation] sak (150 mL) at 0°C. After on of the base the reaction mixture was stirred at room tempera- ture for 18 h. The reaction e was poured into water and extracted with methylene chlo- ride (2x50 mL). The aqueous layer was acidified with 10% HCI. The ing mixture was ex- d with methylene chloride (2x150 mL). The combined organic layers were dried over N82804 and evaporated to dryness to give rac-trans(3-fluoro-phenyl)- cyclopropanecarboxylic acid as colorless crystals (9.5 g, 85%). Separation of the isomers by chiral SFC (Method D) gave the title compound (1S,2S)—2-(3-fluoro-pheny|)- cyclopropanecarboxylic acid IM47 as colorless crystals (3.27 g, 17% overall yield from 3- fluorostyrene) iently pure for the next step. Specific optical rotation +263.4° [or]D20 (C = 1% MeOH) IM48: (1 S, 2S)(4-Fluoro-phenyl)-cyclopropanecarboxy/ic acid F : 0H Prepared analogously to IM48 using SFC method E to give the title compound sufficiently pure for the next step (3.1 g, 13% overall yield from 4-fluorostyrene). Specific optical rotation +263.2° MD” (0 = 1% MeOH) Other intermediates.
IM49: (R)Methyl-propanesu/finic acid [2-(terf-butyl-dimethyl-silanyloxy)-ethylidene]— amide K/O;Si/ (R)—(+)methylpropanesulfinamide (8.70 g, 71.8 mmol), pyridinium p-toluenesulfonate (0.902 g, 3.59 mmol) and MgSO4 (43.2 g, 359 mmol) was ded in methylene chloride (25 mL). A solution of (terf-butyldimethylsilyloxy)acetaldehyde (25.0 g, 144 mmol) dissolved in methylene chloride (10 mL) was added drop wise at room temperature. The reaction was stirred at room temperature overnight. The mixture was evaporated to dryness. Flash chro- matography (silica, EtOAc/heptanes 1:4) gave the title compound as an oil that slowly solidi- fied upon standing (18.3 g, 92%) sufficiently pure for the next step.
Example 1: Preparation of example nds of the invention: [Annotation] sak Compound 1: (1 S, 2S)-2—Pheny/—cyclopropanecarboxy/ic acid [(8)(6-isopropoxy—pyridin-3— yl)-ethy/]-amide 'E'HZ I"'-V)kNH VAC“ + | k / N 0 AC» k\ Transphenylcyclopropanecarboxylic acid IM46 (0.590 g, 3.64 mmol) was dissolved in DMF (15.0 mL). N,N,N’,N’-tetramethyl-O-(7-azabenzotriazolyl)uronium hexafluorophos- phate (1.38 g, 3.63 mmol) was added. Triethylamine (1.10 mL, 7.89 mmol) was then added and the mixture was stirred for 15 s at room temperature. This mixture was added drop wise to a solution of (S)—1-(6-isopropoxy-pyridinyl)-ethylamine IM24 (0.655 g, 3.63 mmol) dissolved in DMF (15.0 mL) over 2 minutes. The mixture was stirred at room temperature over night. The mixture was evaporated to s. The residue was transferred to a silica gel column and eluded with EtOAc/heptanes 1:1 to give Compound 1 as a solid. This solid was dissolved in EtOAc (50 mL) and to this solution was slowly added es (50 mL). The mixture was concentrated to approx. 25 mL in vacuo and this solution was cooled in an ice/water bath. A white precipitate . The solids was collected by filtration and dried in vacuo to give the title compound as colorless crystals (0.794 g, 67%). LC-MS (m/z) 325.4 (MH+), tR = 1.51 min (method A). 1H NMR (500 MHz, DMSO) 6 8.57 — 8.50 (m, 1H), 8.06 (br s, 1H), 7.65 — 7.57 (m, 1H), 7.30 — 7.24 (m, 2H), 7.20 — 7.14 (m, 1H), 7.10 (d, J = 7.5 Hz, 2H), 6.69 (d, J = 8.5 Hz, 1H), 5.25 — 5.16 (m, 1H), 4.98 — 4.88 (m, 1H), 2.24 — 2.15 (m, 1H), 1.94 — 1.88 (m, 1H), 1.41 — 1.32 (m, 4H), 1.26 (d, J = 6.2 Hz, 6H), 1.20 (ddd, J = 8.5, 6.1, 4.1 Hz, 1H). Diastereomeric excess >95% based on 1H NMR.
Compound 2: (1 2—Pheny/—cyclopropanecarboxy/ic acid -(5-methy/-pyridin-2—y/)- ethyIJ-amide o o 2 VAN“ I""V)LOH + N \ l N-(3-Dimethylaminopropyl)—N’-ethylcarbodiimide hydrochloride (0.709 g, 3.70 mmol) and 1- hydroxybenzotriazole (0.667 g, 4.93 mmol) were added to a stirred mixture of IM46 (0.60 g, 3.70 mmol) and commercially available (S)—1-(5-methyl-pyridinyl)—ethylamine hydrochloride [Annotation] sak (Supplier Netchem Inc, Catalog No 528193) (0.426 g, 2.47 mmol) and N,N- diisopropylethylamine (0.859 ml, 4.93 mmol) in THF (25 ml). The solution was stirred at rt overnight. Water was added and the mixture was extracted with EtOAc (3x80 ml). The com- bined organic phases were washed with brine, dried over MgSO4, filtered and the solvent was evaporated of in vac. The crude product was purified by silica gel tography (EtOAc in heptanes 1:1). Yield of Compound 2: 110 mg (16%). LC-MS (m/z) 281.1 (MH+), tR = 0.91 min (method A).
Compound 3: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(S)(6-methoxy—pyridin-B—yD- ethyIJ-amide NHz ,, O NH kOH / IN —> E \ 0/ | \ / Prepared analogously to Compound 1 using IM46 and commercially available (S)—1-(6- methoxy-pyridinyl)-ethylamine (Supplier m Inc, Catalog No 517706). Yield = 0.88 g (66%).1H NMR (500 MHz, DMSO) 6 8.55 (d, 1H), 8.10 (s, 1H), 7.63 (d, 1H), 7.27 (m, 2H), 7.17 (m, 1H), 7.10 (d, 2H), 6.78 (d, 1H), 4.93 (m, 1H), 3.72 (s, 3H), 2.21 (m, 1H), 1.90 (m, 1H), 1.37 (m, 4H), 1.20 (m, 1H). LC-MS (m/z) 297.4 (MH+), tR = 1.36 min (method A).
Compound 4: (1 S,2S)-2—Pheny/—cyclopropanecarboxy/ic acid [(S)(6-methyl—pyridin-3—y/)— ethyIJ-amide NHz ,, :VAOH 0QEO NH / N —> E + n\ Prepared ously to Compound 1 using IM46 and commercially available (S)—1-(6- methyl-pyridinyl)-ethylamine (Supplier m Inc, Catalog No 519526). Yield = 0.27 g (21%).1H NMR (600 MHz, DMSO) 6 8.61 (d, 1H), 8.37 (s, 1H), 7.57 (d, 1H), 7.26 (m, 2H), 7.16 (m, 2H), 7.09 (d, 2H), 4.92 (m, 1H), 2.41 (s, 3H), 2.19 (m, 1H), 1.91 (m, 1H), 1.36 (m, 4H), 1.20 (m, 1H). LC-MS (m/z) 281.2 (MH+), tR = 0.86 min (method A).
[Annotation] sak Compound 5: (1 S, 2S)Pheny/—cyclopropanecarboxy/ic acid [(S)(6-cyano-pyridiny/)— ethyIJ-amide NHz ,, [::j i '" o NH #OH /\©\/ N —> ? / |N \\N \ Prepared ously to Compound 2 using IM46 and cially ble 5-((S) amino-ethyl)-pyridinecarbonitrile hydrochloride (Supplier Netchem Inc, Catalog No 549493). Yield = 0.104 g (20%). %). 1H NMR (600 MHz, DMSO) 6 8.76 (d, 1H), 8.71 (s, 1H), 8.02 (d, 1H), 7.94 (d, 1H), 7.29 (m, 2H), 7.19 (m, 1H), 7.12 (d, 2H), 5.03 (m, 1H), 2.21 (m, 1H), 1.92 (m, 1H), 1.40 (d, 3H), 1.37 (m, 1H), 1.23 (m, 1H). LC-MS (m/z) 292.0 (MH+), tR = 1.31 min (method A).
Compound 6: (1 S,2S)Pheny/—cyclopropanecarboxy/ic acid [(S)(6-trifluoromethyl-pyridin- 3—y/)-ethy/]—amide Ell-l2 ,, O NH “VAOH N —> ? ' / \ F r F \ F F F Prepared analogously to Compound 2 using IM46 and commercially available (S)—1-(6- trifluoromethyl-pyridinyl)—ethylamine hydrochloride. (Supplier Netchem Inc, Catalog No 517662). Yield = 0.33 g (55%). %). 1H NMR (500 MHz, DMSO) 6 8.77 (d, 1H), 8.72 (s, 1H), 7.99 (d, 1H), 7.88 (d, 1H), 7.27 (m, 2H), 7.17 (m, 1H), 7.13 (d, 2H), 5.05 (m, 1H), 2.20 (m, 1H), 1.93 (m, 1H), 1.41 (d, 3H), 1.37 (m, 1H), 1.23 (m, 1H). LC-MS (m/z) 335.2 (MH+), tR = 1.59 min (method A).
Compound 7: (1 S, 2S)Pheny/—cyclopropanecarboxy/ic acid [(S)(6-ethoxy—pyridin-3—yl)- -amide gHZ ----:7/JLNH oowe [Annotation] sak Prepared analogously to Compound 2 using IM46 and commercially available (S)—1-(6- ethoxy-pyridinyl)—ethylamine hydrochloride ier Sial Gmbh, Catalog No 528261-HCI, Lot no LNA098). Yield = 0.30 g (39%). 1H NMR (500 MHz, DMSO) 6 8.54 (d, 1H), 8.08 (s, 1H), 7.62 (d, 1H), 7.27 (m, 2H), 7.16 (m, 1H), 7.12 (d, 2H), 6.73 (d, 1H), 4.92 (m, 1H), 4.25 (q, 2H), 2.20 (m, 1H), 1.91 (m, 1H), 1.35 (m, 4H), 1.30 (t, 3H), 1.19 (m, 1H). LC-MS (m/z) 311.4 (MH+), tR = 1.45 min (method A).
Compound 8: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(S)(6-ethy/-pyridin-3—yl)- ethyIJ-amide NH2 ,, O NH 1"'V)LOH + IN —> ? \ IN Prepared analogously to Compound 1 using IM46 and IM25. Yield = 0.44 g (65%). 1H NMR (500 MHz, DMSO) 6 8.51 (d, 1H), 8.41 (s, 1H), 7.27-7.14 (m, 4H), 7.10 (d, 2H), 4.96 (m, 1H), 2.72 (q, 2H), 2.20 (m, 1H), 1.92 (m, 1H), 1.38 (m, 4H), 1.21 (m, 4H). LC-MS (m/z) 295.1 (MH+), tR = 0.90 min d A). nd 9: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(S)(6-methoxymethyl— pyridinyl)-ethyl]—amide W12 ,, O NH I""V)LOH + IN —> E \ 0\ IN \ o\ Prepared analogously to Compound 1 using IM46 and IM26. Yield = 0.18 g (40%). 1H NMR (500 MHz, DMSO) 6 8.63 (d, 1H), 8.10 (s, 1H), 7.71 (d, 1H), 7.37 (d, 1H), 7.27 (m, 2H), 7.17 (m, 1H), 7.10 (d, 2H), 4.97 (m, 1H), 3.37 (s, 3H), 2.21 (m, 1H), 1.93 (m, 1H), 1.39 (m, 4H), 1.20 (m, 1H). LC-MS (m/z) 311.3 (MH+), tR = 0.98 min (method A).
Compound 10: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid {(S)[6-(2, 2, 2—trifluoro- ethoxy)-pyridinyl]-ethy/}-amide [Annotation] sak NH2 ,, o '5‘“ V» 2 . N OH \ F Io/kF Prepared analogously to Compound 1 using IM46 and IM27. Yield = 0.695 g (50%). 1H NMR (500 MHz, DMSO) 6 8.62 (d, 1H), 8.12 (s, 1H), 7.73 (d, 1H), 7.27 (m, 2H), 7.15 (m, 1H), 7.10 (d, 2H), 6.95 (d), 4.97 (m, 3H), 2.20 (m, 1H), 1.91 (m, 1H), 1.37 (m,4H), 1.20 (m, 1H). LC-MS (m/z) 365.3 (MH+), tR = 1.78 min (method A).
Compound 1 1 : (1 S,28)-2—Phenyl—cyclopropanecarboxylic acid {(8)[6-(2-methoxy—ethoxy)- pyridin-3—y/]—ethy/}-amide E'HZ ”””#NH o0 ' Prepared analogously to Compound 1 using IM46 and IM28. Yield = 0.758 g (30%). 1H NMR (500 MHz, DMSO) 6 8.57 (d, 1H), 8.08 (s, 1H), 7.65 (d, 1H), 7.26 (m, 2H), 7.17 (m, 1H), 7.10 (d, 2H), 6.78 (d, 1H), 4.92 (m, 2H), 4.32 (m, 2H), 3.62 (m, 2H), 3.27 (s, 3H), 2.20 (m, 1H), 1.89 (m, 1H), 1.37 (m, 4H), 1.20 (m, 1H). LC-MS (m/z) 341.0 (MH+), tR = 1.33 min (method A).
Compound 12: (1 S, 28)-2—Phenyl—cyclopropanecarboxylic acid [(S)(2-ethoxy—pyridinyl)- -amide Isz K E:- O = O NH K / —> ? + I O OH \ N | Prepared ously to Compound 1 using IM46 and IM29. Yield = 0.11 g (48%). 1H NMR (500 MHz, DMSO) 6 8.55 (d, 1H), 8.06 (s, 1H), 7.30 (m, 2H), 7.19 (m, 1H), 7.14 (m, 2H), 6.90 (d, 1H), 6.67 (d, 1H), 4.88 (m, 1H), 4.26 (m, 2H), 2.20 (m, 1H), 1.96 (m, 1H), 1.40-1.27 (m, 7H), 1.22 (m, 1H). LC-MS (m/z) 311.4 (MH+), tR = 1.36 min (method A).
[Annotation] sak Compound 13: (1 S, 2S)Pheny/—cyclopropanecarboxy/ic acid ((S){6—[(S)—(tetrahydro- furan-3—yl)ony-pyridin-3—yI}-ethy/)-amide NHz ,, [ 1 O NH / N O _, E / N O OH Q .Q 0‘ \I D Prepared ously to Compound 1 using IM46 and IM30. Yield = 0.983 g (62%). 1H-NMR (500 MHz, DMSO) 6 8.55 (d,1H), 8.09 (s, 1H), 7.64 (d, 1H), 7.27 (m, 2H), 7.16 (m, 1H), 7.11 (d, 2H), 6.78 (d, 1H), 5.48 (m, 1H) 4.96 (m, 1H), 3.92 (m, 1H), 3.85 (m, 1H), 3.74 (m, 2H), 2.21 (m, 2H), 1.95 (m, 2H), 1.37 (m, 4H),1.19 (m, 1H). LC-MS (m/z) 353.3 (MH+), tR = 1.45 min (method A). nd 14: (1 S, 2S)Pheny/—cyclopropanecarboxy/ic acid ((S){6—[(R)—(tetrahydro- furan-3—yl)ony-pyridin-3—yI}-ethy/)-amide Prepared analogously to Compound 1 using IM46 and IM31. Yield = 0.696 g (62%). 1H-NMR (500 MHz, DMSO) 6 8.56 (d,1H), 8.08 (s, 1H), 7.63 (d, 1H), 7.27 (m, 2H), 7.15 (m, 1H), 7.10 (d, 2H), 6.78 (d, 1H), 5.47 (m, 1H) 4.92 (m, 1H), 3.91 (m, 1H), 3.84 (m, 1H), 3.73 (m, 2H), 2.20 (m, 2H), 1.95 (m, 2H), 1.37 (m, 4H),1.20 (m, 1H). LC-MS (m/z) 353.3 (MH+), tR =1.45 min (method A).
Compound 15: (1 S, 2S)Pheny/—cyclopropanecarboxy/ic acid ((S)[1, 3]dioxo/o[4, 5- b]pyridinyI-ethyI)-amide MHZ ,, O NH 1"'V)LOH / + IN a \ IN O \ 04 O ed analogously to Compound 1 using IM46 and IM32. Yield = 0.572 g (42%). 1H-NMR (500 MHZ, DMSO) 6 8.54 (d,1 H), 7.52 (s, 1H), 7.27 (m, 2H), 7.20-7.15 (m, 2H), 7.10 (d, 2H), [Annotation] sak 6.11 (s, 2H), 4.91 (m, 1H), 2.20 (m, 1H), 1.88 (m, 1H), 1.35 (m, 4H),1.20 (m, 1H). LC-MS (m/z) 311.1 (MH+), 1R = 0.61 min (method B).
Compound 16: (1 S, 2S)-2—Pheny/—cyclopropanecarboxy/ic acid [(S)(2, 3-dihydro- [1, 4]dioxino[2, 3-b]pyridin- 7—y/)-ethyl]—amide Ell-l2 ,, o NH «L + flN —> OH \ o | o¢ 0 Prepared analogously to Compound 1 using IM46 and IM33. Yield = 0.877 g (39%). 1H-NMR (500 MHz, DMSO) 6 8.52 (d,1H), 7.68 (s, 1H), 7.27 (m, 2H), 7.22 (s, 1H), 7.18 (m, 1H), 7.10 (d, 2H), 4.92 (m, 1H), 4.37 (d, 2H), 4.22 (d, 2H), 2.20 (m, 1H), 1.90 (m, 1H), 1.35 (m, 4H),1.20 (m, 1H). LC-MS (m/z) 325.5 (MH+), 1R = 1.28 min d A).
Compound 17: (1 S, 28)PhenyI-cyclopropanecarboxy/ic acid [(S)(2-ethoxy-pyrimidin yl)-ethy/]-amide NHz ,, O NH W ; + YINmag fl/ \N o/\ Prepared analogously to Compound 1 using IM46 and IM34. Yield = 0.195 g (32%). 1H-NMR (600 MHz, DMSO) 6 8.53 , 8.52 (s, 2H), 7.26 (m, 2H), 7.16 (s, 1H), 7.10 (d, 2H), 4.92 (m, 1H), 4.32 (m, 2H), 2.20 (m, 1H), 1.88 (m, 1H), 1.40 (d, 3H), 1.35 (m, 1H), 1.31 (t, 3H),1.20 (m, 1H). LC-MS (m/z) 312.2 (MH+), tR = 0.62 min d B).
Compound 18: (1 S, 2S)-2—Pheny/—cyclopropanecarboxy/ic acid [(S)(6-ch/oro-pyridinyl)- ethyIJ-amide NHz ,, O NH + /\CNK ' OH \ Cl | [Annotation] sak Prepared analogously to Compound 1 using IM46 and commercially available (S)—1-(6- Chloro-pyridinyl)-ethylamine a building blocks catalogue nrA06.814.555). Yield = 1.40 g (71%). 1H-NMR (500 MHz, DMSO) 6 8.68 (d,1H), 8.36 (s, 1H), 7.78 (d, 1H), 7.48 (d, 1H), 7.28 (m, 2H), 7.18 (m, 1H), 7.11 (d, 2H), 4.99 (m, 1H), 2.20 (m, 1H), 1.92 (m, 1H), 1.37 (m, 4H),1.20 (m, 1H). LC-MS (m/z) 301.2 and 303.1 (MH+), tR = 0.67 min (method B).
Compound 19: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid {(S)[6-(oxetany/oxy)- pyridin-3—y/]—ethy/}-amide © 0 O 0 %w + L —» \I \ Cl 0 (1 S,2S)Phenyl-cyclopropanecarboxylic acid [(S)—1-(6-chloro-pyridinyl)-ethyl]-amide (Compound 18) (2.00 g, 6.65 mmol) was dissolved in DMF (50 ml). Oxetanol (4.00 g, 54 mmol) and dicesium carbonate (16.2 g, 49.9 mmol) were added and the mixture was heated at 100 °C ON. The mixture was poured out into brine and ted with EtOAc. The organic layer was washed with brine, dried (MgSO4) and was filtered and then evaporated to dry- ness. The residue was transferred to a silica gel column and eluded with EtOAc/heptanes 1:1 to give Compound 19 as a solid. This solid was dissolved in a mixture of THF (10 ml), EtOAc (20 mL) and heptanes (10 ml).The mixture was trated to approx. 15 mL in vacuo and this solution was cooled in an ice/water bath. A white precipitate formed. The solids was col- lected by tion and dried in vacuo to give the title compound as colorless crystals (0.097 g, 4%). %). 1H-NMR (500 MHz, DMSO) 6 8.55 , 8.04 (s, 1H), 7.70 (d, 1H), 7.25 (m,2H), 7.15 (m, 1H), 7.10 (d, 2H), 6.87 (d, 1H), 5.52 (m, 1H), 4.95 (m, 1H), 4.87 (m, 2H), 4.53 (m, 2H), 2.20 (m, 1H), 1.90 (m, 1H), 1.37 (m, 4H),1.21 (m, 1H). LC-MS (m/z) 339.2 (MH+), tR = 0.63 min (method B). Mp =151-153 C. nd 20: (1 S,28)-2—Pheny/—cyclopropanecarboxy/ic acid [(S)(6—cyanomethoxy— pyridin-3—yl)-ethyl]—amide ation] sak (>11 " 0 = OH / VA'2‘“ + IN I _’ = OH Compound 20a G#0630"r ©Vin Compound 20b Compound 20 Compound 20a was ed analogously to Compound 1 using IM46 and IM35 for the first step. Yield of nd 20a = 1.50 g (71%). Pure on TLC (Eluent EtOAc, Rf = 0.5). This material (1.50 g, 4.36 mmol) was dissolved in a mixture of DMSO (50 ml) and THF (50 ml) and 1-Hydroxy-1,2-benziodoxol-3(1H)—one 1-Oxide (1.34 g, 4.8 mmol) was added and the mixture was stirred at rt overnight. The mixture was added to a mixture of brine and EtOAc and the phases were separated. The organic phases was dried over MgSO4 and was ro- tovaped. The crude product was purified by silica gel chromatography (Eluent EtOAc). Yield of Compound 20b = 1.08 g (72%). Pure on TLC (Eluent EtOAc, Rf = 0.7). This material (1.05 g, 3.24 mmol) was dissolved in THF (10 ml), and MeCN (50 ml) and added drop wise to a so- lution of 1-Hydroxy-1,2-benziodoxol-3(1H)—one e (1.36 g, 4.86 mmol) in ammonia in water (13 M, 25 ml) and THF (5 ml) and MeCN (5 ml). The mixture was stirred at rt ON.
The organic phases was dried over MgSO4 and was rotovaped. The crude product was puri- fied by silica gel chromatography (Eluent EtOAc in heptanes 4:1) to give 177 mg impure prod- uct. This material was THF (10 ml) and EtOAc (10 ml) and heptanes (10 ml) were added. The mixture was rotovaped until aprox 10 ml solvent was left and then cooled in an ice bath. A solid precipitated and was collected and dried in vac. Yield of Compound 20 = 0.054 g (5%).
Pure on LCMS and H-NMR. Mp—- 163-165 C. LC-MS (m/z) 322.1 (MH+), tR= 1.03 min (method A). 1H-NMR (500 MHz, DMSO) 6 8.60 , 8.17 (s, 1H), 7.75 (d, 1H), 7.27 (m, 2H), 7.17 (m, 1H), 7.11 (d, 2H), 6.95 (d, 1H), 5.19 (s, 2H), 4.97 (m, 1H), 2.21 (m, 1H), 1.92 (m, 1H), 1.38 (m, 4H),1.21 (m, 1H). nd 21: (1 S, 2S)Phenyl—cyclopropanecarboxy/ic acid [(R)hydroxy—1-(6-propoxy— pyridin-3—y/)-ethy/]—amide [Annotation] sak 6 z '- o NH OH w y 4 OH \I O OH(U f\ Triethylamine (0.384 mL, 2.75 mmol) was added to a mixture of 2-phenyl cyclopropanecarboxylic acid IM46 (223 mg, 1.38 mmol) and O-(benzotriazolyl)-N,N,N’,N’- tetramethyluronium hexafluorophosphate (522 mg, 1.38 mmol) suspended in DMF (2mL) in a small vial. The vial was vigorously agitated for 30 seconds and then left for 5 minutes. This mixture was added drop wise to (R)—2-Amino(6-propoxy-pyridinyl)-ethanol IM36 (270 mg, 1.4 mmol) dissolved in DMF (3 mL). After 1 h the mixture was poured into a mixture of EtOAc (40 mL) and brine (20 mL). The organic layer was dried over MgSO4 and evaporated to dryness. Flash tography (silica, 10-100% EtOAc in heptanes) gave the title com- pound as a white solid (0.134 g, 29%). LC-MS (m/z) 341.0 (MH+), tR = 1.52 min (method A). 1H-NMR (600 MHz, DMSO) 6 8.53 (d, J = 8.2 Hz, 1H), 8.04 (br s, 1H), 7.64 — 7.57 (m, 1H), 7.30 — 7.23 (m, 2H), 7.19 — 7.14 (m, 1H), 7.10 (d, J = 7.3 Hz, 2H), 6.74 (d, J = 8.5 Hz,1H), 4.96 — 4.90 (m, 1H), 4.88 — 4.80 (m, 1H), 4.16 (t, J = 6.7 Hz, 2H), 3.61 — 3.49 (m, 2H), 2.23 — 2.15 (m, 1H), 2.04 — 1.95 (m, 1H), 1.74 — 1.64 (m, 2H), 1.40 — 1.32 (m, 1H), 1.23 — 1.13 (m, 1H), 0.94 (t, J = 7.4 Hz, 3H).
Compound 22: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(R)hydroxy—1-(6- trifluoromethyl—pyridinyl)-ethyl]-amide NH2 ’1. 6 z '- o NH ""-V)LOH + w 2 ' / OH \ F IN OH \ F N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (0.39 g, 2.03 mmol) and 1- hydroxybenzotriazole (0.366 g, 2.71 mmol) were added to a stirred mixture of IM46 (0.22 g, 1.36 mmol) and commercially ble (R)—2-amino(6-trifluoromethyl-pyridinyl)-ethanol hydrochloride (Supplier Netchem Inc, Catalog No 517882) (0.494 g, 2.03 mmol) and N,N- diisopropylethylamine (0.472 ml, 2.71 mmol) in THF (20 ml). The solution was d at rt overnight. Water was added and the mixture was extracted with EtOAc (3x80 ml). The com- bined organic phases were washed with brine, dried over MgSO4, filtered and the solvent was evaporated of in vac. The crude product was purified by silica gel chromatography [Annotation] sak (EtOAc in heptanes 10:1). Yield of Compound 22 475 mg (78%). 1H-NMR (500 MHz, DMSO) 6 8.77 (d, 1H), 8.74 (s, 1H), 8.00 (d, 1H), 7.88 (d, 1H), 7.27 (m, 2H), 7.18 (m, 1H), 7.13 (d, 2H), 5.04 (m, 2H), 3.64 (m, 2H), 2.22 (m, 1H), 2.07 (m, 1H), 1.37 (m, 1H), 1.37 (m, 1H), 1.23 (m, 1H). LC-MS (m/z) 351.1 (MH+), tR = 1.51 min (method A).
Compound 23: (1 S,28)-2—Pheny/—cyclopropanecarboxy/ic acid [(R)(5-cyano-pyridin-2—yl)— 2—hydroxy—ethy/J—amide I l /N OH OH \ \ | \ N OH \ Prepared analogously to Compound 22 using IM46 and commercially available 6-((R)—1- aminohydroxy-ethyl)-nicotinonitrile (Supplier Netchem Inc., Catalog No ). Yield of Compound 23 230 mg (61%). 1H-NMR (500 MHz, DMSO) 6 8.97 (s, 1H), 8.72 (d, 1H), 8.26 (d, 1H), 7.52 (d, 1H), 7.27 (m, 2H), 7.18 (t, 1H), 7.11 (d, 2H), 5.05-4.95 (m, 2H), 3.70 (m, 2H), 2.20 (m, 1H), 2.17 (m, 1H), 1.36 (m, 1H), 1.20 (m, 1H). LC-MS (m/z) 308.1 (MH+), tR = 1.21 min (method A).
Compound 24: (1 S, 28)-2—Phenyl-cyclopropanecarboxy/ic acid [(R)hydroxy—1-(6-methoxy— pyridin-3—yl)-ethyl]—amide MHZ ,, o NH '-V)LOH N s + | / N OH \ 0/ I OH \ / Prepared analogously to nd 22 using IM46 and commercially available (R)—2-Amino- ethoxy-pyridinyl)—ethanol (Supplier Netchem Inc., g No 517926). Yield of Compound 24 = 763 mg (22%). 1H-NMR (500 MHz, DMSO) 6 8.53 (d, 1H), 8.09 (s, 1H), 7.63 (d, 1H), 7.27 (m, 2H), 7.15 (m, 1H), 7.12 (m, 2H), 6.77 (d, 1H), 4.95 (m, 2H), 4.85 (m, 2H), 3.83 (s, 3H), 3.57 (m, 2H), 2.21 (m, 1H), 2.00 (m, 1H), 1.37 (m, 1H), 1.19 (m, 1H). LC- MS (m/z) 313.1 (MH+), tR = 1.53 min (method A).
Compound 25: (1 S,28)-2—Pheny/—cyclopropanecarboxy/ic acid [(R)hydroxy—1-(6-methyl- pyridin-3—yl)-ethyl]—amide [Annotation] sak 0 © o EDC.HCI ©V)kIIIIII#NH'-#NH 5 «k _ _’ —> / N OH N - | I LIOH 0H \ ”Q C compound 25a N-(3-Dimethylaminopropyl)—N’-ethylcarbodiimide hydrochloride (0.316 g, 1.65 mmol) and 1- hydroxybenzotriazole (0.223 g, 1.65 mmol) were added to a stirred mixture of IM46 (0.18 g, 1.1 mmol) and commercially available (R)—2-Amino(6-methyl-pyridinyl)-ethanol dihydro- chloride (Supplier Netchem Inc., Catalog No 549945) (0.128 g, 1.21 mmol) and N,N- diisopropylethylamine (0.575 ml, 3.30 mmol) in THF (10 ml). The solution was stirred at rt overnight. Water was added and the mixture was extracted with EtOAc. The organic phase was rotovaped to produce 140 mg of Compound 25a. LC-MS (m/z) 441.4 (MH+), tR = 1.44 min (method A). Compound 25a was dissolved in THF and LiOH (1 M) was added and the mixture was stirred 30 min. A solid precipitated and was isolated by filtration and dried in vac.
Yield of Compound 25 = 110 mg (34%). 1H-NMR (500 MHZ, DMSO) 6 8.53 (d, 1H), 8.09 (s, 1H), 7.63 (d, 1H), 7.27 (m, 2H), 7.15 (m, 1H), 7.12 (m, 2H), 6.77 (d, 1H), 4.95 (m, 2H), 4.85 (m, 2H), 3.83 (s, 3H), 3.57 (m, 2H), 2.21 (m, 1H), 2.00 (m, 1H), 1.37 (m, 1H), 1.19 (m, 1H).
LC-MS (m/z) 297.3 (MH+), tR = 0.78 min d A).
Compound 26: (1 S,28)Pheny/—cyclopropanecarboxy/ic acid -hydroxy—1-(6- isopropoxy—pyridinyI)-ethy/]-amide Prepared analogously to Compound 21 using IM46 and IM37. Yield of nd 26 = 667 mg (77%). 1H-NMR (600 MHz, DMSO) 6 8.52 (d, 1H), 8.03 (s, 1H), 7.60 (d, 1H), 7.25 (m, 2H), 7.15 (m, 1H), 7.10 (d, 2H), 6.67 (d, 1H), 5.20 (m, 1H), 4.92 (t, 1H), 4.83 (m, 1H), 3.55 (m, 2H), 2.20 (m, 1H), 2.00 (m, 1H), 1.35 (m, 1H), 1.24 (d, 6H), 1.19 (m, 1H). LC-MS (m/z) 341.0 (MH+), tR = 1.45 min (method A).
[Annotation] sak Compound 27: (1 S,2S)(3—F/uoro-pheny/)-cyc/opropanecarboxylic acid [(R)(6-ethoxy- pyridin-3—y/)hydroxy-ethyl]—amide NH2 - 6 g F o 51H I""V)kOH + F OH \' N J )0 OH \ Prepared analogously to Compound 21 using IM47 and IM38. Yield of Compound 27 = 90 mg (19%). 1H-NMR (600 MHz, DMSO) 6 8.53 (d, 1H), 8.05 (s, 1H), 7.62 (m, 1H), 7.28 (m, 1H), 6.97 (m, 3H), 6.73 (d, 1H), 4.83 (m, 1H), 4.24 (m, 2H), 3.55 (m, 2H), 2.22 (m, 1H), 2.03 (m, 1H), 1.37 (m, 1H), 1.28 (t, 3H), 1.22 (m, 1H). LC-MS (m/z) 345.0 (MH+), tR = 0.63 min (method B).
Compound 28: (1 S,2S)(4-F/uoro-pheny/)-cyc/opropanecarboxylic acid [(R)(6-ethoxy- pyridin-3—y/)hydroxy-ethyl]—amide MHZ , VA F ; NH / E OH OH \ O I OH \ J Prepared analogously to Compound 21 using IM48 and IM38. Yield of Compound 28 = 93 mg white solid. (54%). 1H-NMR (600 MHz, DMSO) 6 8.56 (d, 1H), 8.07 (s, 1H), 7.62 (m, 1H), 7.18 (m, 2H), 7.12 (m, 2H), 6.77 (d, 1H), 4.93 (t, 1H), 4.83 (m, 1H), 4.25 (dd, 2H), 3.52 (m, 2H), 2.27 (m, 1H), 1.97 (m, 1H), 1.30 (m, 4H), 1.15 (m, 1H). LC-MS (m/z) 345.0 (MH+), LCMS (m/z) 345.0 (MH+), tR = 1.36 min (method A).
Compound 29: (1 2-(3—F/uoro-pheny/)-cyc/opropanecarboxylic acid [(R)hydroxy (6-propoxy-pyridin-3—yl)-ethyl]-amide JG a F '- o NH ""-V)L0H + —» / F OH \' O IN OH \ J/ ed analogously to Compound 21 using IM47 and IM36. Yield = 150 mg (30%). 1H- NMR (600 MHz, DMSO) 6 8.53 (d, 1H), 8.03 (s, 1H), 7.62 (d, 1H), 7.29 (m, 1H), 6.95 (m, 3H), [Annotation] sak 6.74 (d, 1H), 4.93 (m, 1H), 4.84 (m, 1H), 4.16 (t, 2H), 3.55 (m, 2H), 2.23 (m, 1H), 2.03 (m, 1H), 1.68 (m, 2H), 1.37 (m, 1H), 1.25 (m, 1H), 0.93 (t, 3H). LC-MS (m/z) 359.1 (MH+), tR = 1.57 min (method A).
Compound 30: (1 S, 2S)(4-F/uoro-phenyI)-cyclopropanecarboxy/ic acid -hydroxy (6-propoxy-pyridinyl)-ethyl]-amide F ,, (3 ? o NH W»... mN+ J/ 3 O /, f OH \ Prepared analogously to Compound 21 using IM48 and IM36. Yield 176 mg (36%). 1H-NMR (600 MHz, DMSO) 6 8.52 (d, 1H), 8.05 (s, 1H), 7.63 (d, 1H), 7.14 (m, 2H), 7.09 (m, 2H), 6.74 (d, 1H), 4.92 (t, 1H), 4.85 (m, 1H), 4.15 (t, 2H), 3.55 (m, 2H), 2.22 (m, 1H), 1.97 (m, 1H), 1.70 (m, 2H), 1.34 (m, 1H), 1.18 (m, 1H), 0.92 (t, 3H).
Compound 31: (1 S, 2S)Pheny/—cyclopropanecarboxy/ic acid [(R)(6-(2, 2, 2-d3)-ethoxy- pyridin-3—y/)hydroxy-ethyl]—amide [EH—'2 """#NH #0 a + “Q o / H OH \ O/\)/D ' D Prepared ously to Compound 21 using IM46 and IM40. Yield = 785 mg (66%). 1H- NMR (600 MHz, CDCI3) 6 8.62 (s, 1H), 7.52 (d, 1H), 7.26 (m, 2H), 7.20 (m, 1H), 7.08 (d, 2H), 6.71 (d, 1H), 6.27 (m, 1H), 5.05 (m, 1H), 4.31 (s, 2H), 3.91 (m, 2H), 2.51 (m, 2H), 1.67 (m, 2H), 1.31 (m, 1H). LC-MS (m/z) 330.3 (MH+), tR = 1.32 min (method A).
Compound 32: (1 S, 2S)(3-F/uoro-phenyI)-cyclopropanecarboxy/ic acid [(R)(6-(1,1-d2)- ethoxy-pyridinyl)hydroxy-ethyl]—amide [Annotation] sak Prepared analogously to Compound 21 using IM47 and IM41. Yield = 104 mg (44%). 1H- NMR (600 MHz, DMSO) 6 8.52 (d, 1H), 8.03 (s, 1H), 7.61 (m, 1H), 7.29 (m, 1H), 6.97 (m, 3H), 6.72 (d, 1H), 4.93 (m, 1H), 4.85 (m, 1H), 3.55 (m, 2H), 2.22 (m, 1H), 2.02 (m, 1H), 1.37 (m, 1H), 1.25 (m, 4H). LC-MS (m/z) 347.2 (MH+), tR = 0.64 min (method A).
Compound 33: (1 S,2S)-2—Pheny/—cyclopropanecarboxy/ic acid [(R)(6-ethoxy—pyridin-3—yl)- 2-hydroxy-ethy/J—amide NH2 ,, o NH '-V)LOH N ? + I / N OH \ /\ O I ed analogously to Compound 22 using IM46 and IM38. Yield = 90 mg (28%). 1H-NMR (600 MHz, DMSO) 6 8.53 (d, 1H), 8.06 (s, 1H), 7.52 (m, 1H), 7.26 (m, 2H), 7.16 (m, 1H), 7.10 (d, 2H), 6.73 (d, 1H), 4.95 (t, 1H), 4.87 (m, 1H), 4.25 (m, 2H), 3.55 (m, 2H), 2.20 (m, 1H), 2.02 (m, 1H), 1.36 (m, 1H), 1.28 (t, 3H), 1.20 (m, 1H). LC-MS (m/z) 327.4 (MH+), tR = 0.57 min d B).
Compound 34: (1 S, 2S)-2—(4-Fluoro-phenyl)-cyc/opropanecarboxy/ic acid [(R)(6-(1, 1,2, 2, 2- d5)-ethoxy-pyridinyl)-2—hydroxy—ethyl]—amide F ,, o 1ng + (\CNKD = D —> 0&0 / N OH OH \ D D Prepared analogously to Compound 21 using IM48 and IM39. Yield = 130 mg (41%). 1H- NMR (600 MHz, DMSO) 6 8.53 (d, 1H), 8.06 (s, 1H), 7.62 (m, 1H), 7.14 (m, 2H), 7.10 (m, 2H), 6.73 (d, 1H), 4.95 (br s, 1H), 4.84 (m, 1H), 3.55 (m, 2H), 2.22 (m, 1H), 1.96 (m, 1H), 1.34 (m, 1H), 1.18 (m, 1H). LC-MS (m/z) 350.2 (MH+), tR = 1.41 min (method A).
Compound 35: (1 S,2S)(4-F/uoro-phenyl)-cyc/opropanecarboxylic acid [(R)(6-(2, 2, 2-d3)- ethoxy-pyridinyl)-2—hydroxy—ethyl]—amide [Annotation] sak Prepared analogously to nd 21 using IM48 and IM40. Yield = 144 mg (41%). 1H- NMR (600 MHz, DMSO) 6 8.53 (d, 1H), 8.08 (s, 1H), 7.63 (m, 1H), 7.13 (m, 2H), 7.10 (m, 2H), 6.73 (d, 1H), 4.97 (br s, 1H), 4.86 (m, 1H), 4.24 (s, 2H), 3.57 (m, 2H), 2.23 (m, 1H), 1.97 (m, 1H), 1.37 (m, 1H), 1.20 (m, 1H). LC-MS (m/z) 347.9 (MH+), tR = 1.39 min (method A).
Compound 36: (1 S, Phenyl—cyclopropanecarboxy/ic acid [(R)(6-(1, 1 -d2)-ethoxy- pyridin-3—y/)hydroxy—ethyl]—amide NHz ,, (3 z O NH / N E + D D I,"-V)LOH I X —> / OH D \ D 0 lN OH \ OX Prepared analogously to Compound 21 using IM46 and IM41. Yield = 148 mg (67%). 1H- NMR (600 MHz, DMSO) 6 8.52 (d, 1H), 8.05 (s, 1H), 7.61 (d, 1H), 7.50 (m, 2H), 7.15 (m, 1H), 7.10 (d, 2H), 6.72 (d, 1H), 4.94 (t, 1H), 4.85 (m, 1H), 3.55 (m, 2H), 2.18 (m, 1H), 1.97 (m, 1H), 1.35 (m, 1H), 1.27 (s, 3H), 1.20 (m, 1H). LC-MS (m/z) 329.2 (MH+), tR = 0.61 min (method B).
Compound 37: (1 S, 2S)-2—(4-F/uoro-phenyI)-cyclopropanecarboxy/ic acid [(R)(6-(1,1-d2)- ethoxy-pyridinyl)-2—hydroxy—ethyl]—amide F ,, o NH [ I"'-V)LOH+C,):\©D>§/ N E D D / O INDD OH \ ox Prepared analogously to Compound 21 using IM48 and IM41. Yield = 123 mg (52%). 1H- NMR (600 MHz, DMSO) 6 8.52 (d, 1H), 8.06 (s, 1H), 7.62 (m, 1H), 7.14 (m, 2H), 7.10 (m, 2H), 6.73 (d, 1H), 4.92 (t, 1H), 4.84 (m, 1H), 3.55 (m, 2H), 2.22 (m, 1H), 1.96 (m, 1H), 1.34 (m, 1H), 1.28 (s, 3H), 1.18 (m, 1H). LC-MS (m/z) 347.2 (MH+), tR = 1.41 min (method B).
[Annotation] sak Compound 38: (1 S, 2S)Pheny/—cyclopropanecarboxy/ic acid [(R)(6-(1, 1,2, 2, 2-d5)- ethoxy-pyridinyl)hydroxy-ethyl]—amide NHZ I «k E D NH O D + N D E D D | —> OH OH \ D O | Prepared analogously to Compound 21 using IM46 and IM39. Yield = 98 mg (32%). 1H-NMR (600 MHz, DMSO) 6 8.52 (d, 1H), 8.05 (s, 1H), 7.61 (d, 1H), 7.26 (t, 2H), 7.16 (t, 1H), 7.10 (d, 2H), 6.72 (d, 1H), 4.93 (t, 1H), 4.83 (m, 1H), 3.55 (m, 2H), 2.18 (m, 1H), 1.99 (m, 1H), 1.35 (m, 1H), 1.20 (m, 1H). LC-MS (m/z) 332.2 (MH+), tR = 0.61 min (method B).
Compound 39: (1 S, 2S)(3-F/uoro-phenyI)-cyclopropanecarboxy/ic acid [(R)(6-(2, 2, 2-d3)- ethoxy-pyridinyl)hydroxy-ethyl]—amide '2le O I F D D / [EIH + IN TD - D ’ —> / ND F OH OH \ 0 l OH \ TD Prepared analogously to Compound 21 using IM47 and IM40. Yield = 101 mg (29%). 1H- NMR (600 MHz, DMSO) 6 8.53 (d, 1H), 8.05 (s, 1H), 7.61 (d, 1H), 7.28 (m, 1H), 70-695 (m, 3H), 6.72 (d, 1H), 4.93 (t, 1H), 4.83 (m, 1H), 4.22 (s, 2H), 3.55 (m, 2H), 2.23 (m, 1H), 2.02 (m, 1H), 1.37 (m, 1H), 1.27 (m, 1H). LC-MS (m/z) 348.0 (MH+), tR = 1.43 min (method A).
Compound 40: (1 2-Pheny/—cyclopropanecarboxy/ic acid [(R)(6-cyclobutoxy-pyridin- 3-yl)hydroxy-ethy/]—amide 3‘" z '- o NH «k / N E + (\Ok D —’ / N OH OH \ 0 ' o/D OH \ ed analogously to Compound 21 using IM46 and IM44. Yield = 53 mg (30%). 1H-NMR (600 MHz, DMSO) 6 8.50 (d, 1H), 8.03 (s, 1H), 7.61 (d, 1H), 7.25 (m, 2H) 7.16 (m, 1H), 7.10 (m, 2H), 6.71 (d, 1H), 5.09 (m, 1H), 4.90 (t, 1H), 4.82 (m, 1H), 3.55 (m, 2H), 2.37 (m, 2H), [Annotation] sak 2.21 (m, 1H), 2.00 (m, 4H), 1.76 (m, 1H), 1.61 (m, 1H), 1.32 (m, 1H), 1.20 (m, -MS (m/z) 353.1 (MH+), tR = 0.70 min (method B).
Compound 41: (1 S,2S)(3-F/uoro-phenyl)-cyc/opropanecarboxylic acid [(R)(6- cyc/obutoxy-pyridiny/)hydroxy-ethy/]—amide NHz ,, g F o NH "'V)kOH m D/+ N ? I, / O IN E OH \ Prepared analogously to Compound 21 using IM47 and IM44. .Yield = 38 mg (20%). 1H- NMR (600 MHz, DMSO) 6 8.50 (d, 1H), 8.03 (s, 1H), 7.61 (d, 1H), 7.27 (m, 1H), 6.95 (m, 3H), 6.71 (d, 1H), 5.08 (m, 1H), 4.95 (br. s, 1H), 4.82 (m, 1H), 3.55 (m, 2H), 2.37 (m, 2H), 2.22 (m, 1H), 2.02 (m, 4H), 1.75 (m, 1H), 1.61 (m, 1H), 1.37 (m, 1H), 1.27 (m, 1H). LC-MS (m/z) 371.1 (MH+), tR = 0.71 min (method B).
Compound 42: (1 S,2S)(4-F/uoro-phenyl)-cyc/opropanecarboxylic acid [(R)(6- cyc/obutoxy-pyridiny/)hydroxy-ethy/]—amide NH2 , Fo 3 NH ,, + —> O OH \ 0 ed analogously to Compound 21 using IM48 and lM44.Yie|d = 84 mg (45%). 1H-NMR (600 MHz, DMSO) 6 8.50 (d, 1H), 8.03 (s, 1H), 7.61 (d, 1H), 7.14 (m, 2H), 7.09 (m, 2H), 6.71 (d, 1H), 5.08 (m, 1H), 4.92 (t, 1H), 4.82 (m, 1H), 3.55 (m, 2H), 2.37 (m, 2H), 2.21 (m, 1H), 2.05-1.95 (m, 4H), 1.75 (m, 1H), 1.61 (m, 1H), 1.32 (m, 1H), 1.17 (m, 1H). LC-MS (m/z) 371.1 (MH+), tR = 0.71 min (method B).
Compound 43: (1 S,28)Pheny/—cyclopropanecarboxylic acid ((R)hydroxy{6-[(R)- (tetrahydro-furany/)oxy]—pyridiny/}-ethyl)-amide O EH2 I"-.V)kNH ©”””” - + / IN 0 —> / N O OH \ ' O OH \ (E) [Annotation] sak Prepared analogously to Compound 21 using IM46 and IM43. Yield = 1.34 g white solid (60%).1H NMR (400 MHz, CDCI3): 6 8.08 (s, 1 H), 7.51 (d, J: 8.4 Hz, 1 H), 7.18-7.25 (m, 3 H), 7.04-7.06 (m, 2 H), 6.68-6.71 (m, 1 H), 6.36-6.39 (m, 1 H), 5.50-5.53(m, 1 H), 5.04- .05(m, 1 H), 3.88-4.01 (m, 6 H), 2.48-2.50 (m, 1 H), 2.21-2.26 (m, 1 H), 2.11-2.13 (m, 1 H), 1.62-1.71 (m, 2 H), 1.27-1.31 (m, 1 H). LC-MS (m/z) 369.2 (MH+), tR = 2.04 min d WXE-ABO1). [61,30 = 178.6 (C=0.2, CHCI3) Compound 44: (1 S, 2S)-N-[(1R)hydroxy— 1-[6-[(38)-tetrahydrofuranyljoxy pyridyljethy/J—2-phenyl-cyclopropanecarboxamide © 0 VAW / O 3 Prepared analogously to Compound 21 using IM46 and IM42. Yield = 2.10 g white solid (50%). 1H NMR (400 MHz, CDCI3): 68.09 (m, 1 H), 7.52-7.55 (m, 1 H), 7.29 (m, 1 H), 7.25- 7.27 (m, 1 H), 7.20-7.21 (m, 1 H), 7.17-7.19 (d, J: 7.2 Hz, 2 H), .73 (d, J: 8.8 Hz, 1 H), 6.34-6.36 (d, J = 7.2 Hz, 1 H), 5.50-5.53(m, 1 H), 5.04-5.05(d, J = 6.8 Hz, 1 H), 3.94-4.03 (m, 2 H), 3.85-3.91 (m, 4 H), 2.48-2.50 (m, 1 H), 2.21-2.26 (m, 1 H), 2.11-2.13 (m, 1 H), 1.62- 1.71 (m, 2 H), 1.27-1.31 (m, 1 H). LC-MS (m/z) 369.2 (MH+), tR = 2.03 min (method WXEABO1 ). [61,30 = 160.9 (c=0.21, CHCI3).
Compound 45: (1 S, 2S)((Z)Methy/ene-penta-2, 4-dienyl)-cyclopropanecarboxy/ic acid {(R)hydroxy—1-[6-(tetrahydro-pyrany/oxy)-pyridinyl]-ethyl}-amide 'E‘Hz O ' + @le / N O : II, —> '- OH OH \' /IN 0 O OH \ ed analogously to Compound 1 using IM46 and IM45. Yield = 2.5 g white solid (44%). 1H NMR ((400 MHz, CDCI3): 68.09 (s, 1 H), .55 (m, 1 H), .28 (m, 1 H), 7.25-7.26 (m, 1 H), 7.20 (m, 1 H), 7.06-7.08 (t, J: 4.2 Hz, 2 H), 6.70-6.72 (d, J = 8.4 Hz, 1 H), 6.34-6.36 (d, J = 7.2 Hz, 1 H), 5.18-5.21(m, 1 H), 5.04-5.05(d, J: 6.8 Hz, 1 H), 3.62-3.99 (m, 4 H), [Annotation] sak 3.56-3.62 (m, 2 H), 2.46-2.50 (m, 1 H), 2.01-2.06 (m, 2 H), 1.74-1.80 (m, 2 H), 1.62-1.70 (m, 2 H), 1.27-1.30 (m, 1 H). [011020 = 144.3 (c = 0.204 g/100mL, CHCI3).
Compound 46: (1 S,28)Pheny/—cyclopropanecarboxy/ic acid [(R)(6-ethoxy—pyridinyl)- 2-methoxy—ethy/J—amide E: 0 0 1,,1'V)k lélH © 1”“?ka Km : / IN NaH (60 % sion in mineral oil) (2.06 g, 51.5 mmol) was suspended in DMF and the reaction vessel was cooled in an ice bath. )Phenyl-cyclopropanecarboxylic acid [(R)(6-ethoxy-pyridinyl)hydroxy-ethyl]—amide (Compound 33) (15 g, 46 mmol) was dissolved in DMF (50 ml) and added drop wise to the sodium hydride suspension at 5-8 C over 20 minutes. The on was stirred 30 minutes. Methyl iodide (3.30 ml, 53.0 mmol) dis- solved in DMF (25 ml) was added drop wise at 5-12 C over 10 minutes and the e was stirred at 7-8 C for 30 minutes. The mixture was added to a brine solution and extracted with EtOAc. The organic layer was washed with more brine, dried (MgSO4) filtered and the sol- vent was evaporated off. The crude product was purified by silica gel chromatography t EtOAc in heptanes 4:1 ). The fractions that contained the t was collected and the sol- vent was removed in vac. The residue was redissolved in THF (50 ml) EtOAc (100 ml) and heptanes (25 ml). The mixture was concentrated until 40 ml remained and cooled in ice. A white solid precipitated and was collected by filtration. Yield: 6.75 g (43%) of Compound 46.
LC-MS (m/z) 341.2 (MH+), tR = 0.64 min (method B). 1H-NMR (500 MHz, DMSO) 6 8.62 (d,1H), 8.08 (br s, 1H), 7.63 (d, 1H), 7.27 (m, 2H), 7.17 (m, 1H), 7.10 (d, 2H), 6.74 (d, 1H), 5.05 (m, 1H), 4.26 (m, 2H), 3.55 — 3.46 (m, 2H), 3.26 (s, 3H), 2.20 (m, 1H), 1.99 (m, 1H), 1.38 (m, 1H), 1.29 (t, 3H), 1.20 (m, 1H).
Compound 47: (1 S, 2S)-N-[(1R)-2—methoxy—1-[6-[(3R)-tetrahydrofuranyl]oxy—3- /Jethy/]phenyl-cyc/opropanecarboxamide E: 0 © 0 [1"1,V)LI¥H ,,,,,'V)LNH (\O O / N O I L) I L) OH \ /O \ O 0 Prepared analogously to Compound 46 using Compound 43 (0.90 g).
[Annotation] sak Yield = 490 mg (53%) as a solid. 1H NMR (400 MHz, CDCI3): 68.12 (d, J = 2.4 Hz, 1 H), 7.54- 7.57 (m, 1 H), 7.25-7.28 (m, 2 H), 7.18-7.21 (m, 1 H), 7.05-7.07 (m, 2 H), 6.68-6.70 (d, J: 8.8 Hz, 1 H), 6.37-6.39 (d, J: 8.0 Hz, 1 H), 5.51-5.54 (m, 1 H), 5.10-5.12(m, 1 H), .04 (m, 4 H), 3.66-3.69 (m, 1 H), 3.60-3.63 (m, 1 H), 3.38 (s, 3 H), 2.45-2.46 (m, 1 H), 2.21-2.26 (m, 1 H), 1.60-1.68 (m, 2 H), 1.25-1.28 (m, 1 H). LC-MS: 1R = 0.53 min (method B), m/z = 383.2 [M + H]+. [011,30 = 188.2 (C=0.176, CHCI3).
Compound 48: (1 S, 2S)-N-[(1R)methoxy[6-[(3S)-tetrahydrofuran-B—y/Joxy pyridy/Jethy/]—2-phenyl-cyc/opropanecarboxamide © 0 0 _> © ,VjLNH #NH I I OH \ [2 o \ / [2 o 0 Prepared analogously to Compound 46 using Compound 44 (841 mg).
Yield = 645 mg (44%) as a solid. 1H NMR (400 MHz, CDCI3): 68.10-8.11 (d, J: 2.4 Hz, 1 H), 7.54-7.57 (m, 1 H), 7.25-7.28 (m, 2 H), .21 (m, 1 H), .07 (m, 2 H), 6.68-6.70 (d, J = 8.8 Hz, 1 H), 6.37-6.39 (d, J: 8.0 Hz, 1 H), 5.51-5.54 (m, 1 H), 5.10-5.12(m, 1 H), 3.87- 4.04 (m, 4 H), 3.66-3.69 (m, 1 H), 3.60-3.63 (m, 1 H), 3.38 (s, 3 H), 2.45-2.46 (m, 1 H), 2.21- 2.26 (m, 1 H), 1.60-1.68 (m, 2 H), 1.25-1.28 (m, 1 H). LC-MS: tR = 0.62 min (method B), m/z = 383.2 [M + H]+. [011,30 = 162.5 (C=0.225, CHCI3).
Compound 49: (1 S,2S)Pheny/—cyclopropanecarboxy/ic acid {(R)methoxy—1-[6- (tetrahydro-pyranyloxy)-pyridiny/]-ethyI}-amide Prepared analogously to Compound 46 using Compound 45. Yield = 343 mg (20%) as a solid. 1H NMR (400 MHz, CDCI3): 68.10-8.11 (d, J: 2.4 Hz, 1 H), 7.54-7.57 (m, 1 H), 7.25- 7.29 (m, 2 H), 7.18-7.21 (m, 1 H), 7.05-7.07 (m, 2 H), 6.66-6.68 (t, J: 4.2 Hz, 1 H), 6.38-6.40 (d, J = 7.2 Hz, 1 H), .21 (m, 1 H), .12(m, 1 H), 3.94-3.99 (m, 2 H), 3.57-3.69 (m, 4 H), 3.37 (s, 3 H), 2.42-2.49 (m, 1 H), .08 (m, 2 H), 1.61-1.79 (m, 4 H), 1.25-1.28 (m, 1 H) d1D2° = 1593 (c = 0.198 g/100mL, . LC-MS (m/z) 383.15 (MH+), 1R = 0.54 min (method B).
[Annotation] sak Compound 50: (1 S, Phenyl—cyclopropanecarboxy/ic acid -[6-(oxetany/oxy)- pyridin-3—y/]—ethy/}-amide © 0 O O VA?“ 1",, 1,", + [L NH —> VA HCI \ / / CI [Tl (1 S,2S)Phenyl-cyclopropanecarboxylic acid [(S)—1-(6-chloro-pyridinyl)-ethyl]-amide (Compound 18) (2.00 g, 6.65 mmol) was dissolved in DMF (40 ml). N,N- dimethylhydroxylaminehydrochloride (5.00 g, 51.2 mmol) and dicesium carbonate (25 g, 76.7 mmol) were added and the mixture was heated at 95 C 3 days. The mixture was poured out into brine and extracted with EtOAc. The organic layer was washed with brine, dried ) and was filtered and then evaporated to dryness. The residue was transferred to a silica gel column and eluded with EtOAc to give Compound 50 as a solid. This solid was dissolved in a mixture of THF (10 ml), EtOAc (10 ml) and heptanes (10 ml).The mixture was concentrated to approx. 10 ml volume and this solution was cooled in an ice/water bath. A white precipitate formed. The solids was collected by filtration and dried in vacuo to give the title compound as a white solid (0.044 g, 2%). 1H-NMR (500 MHz, DMSO) 6 8.43 (d,1H), 8.02 (s, 1H), 7.45 (d, 1H), 7.25 (m,2H), 7.17 (m, 1H), 7.10 (d, 2H), 6.61 (d, 1H), 4.83 (m, 1H), 3.98 (m, 6H), 2.19 (m, 1H), 1.90 (m, 1H), 1.35 (m, 4H),1.21 (m, 1H). LC-MS (m/z) 310.2 (MH+), tR = 0.46 min (method B). Mp 81 C.
Compound 51: (1 S, 2S)-2—Phenyl—cyclopropanecarboxylic acid -(6-ethanesulfonyl -pyridinyl)-ethyl]—amide [>le E: ' 0 = / VAIIZ I ""V)kOH + IN : \ / 0 S x IN 8\ \ fif’f’ ed analogously to Compound 1 using IM46 and (S)—1-(6-Methanesulfonyl-pyridin yl)-ethylamine (prepared from 1-(6-Methanesulfonyl-pyridineyl)—ethanone analogously to IM24, which was prepared from commercially available 5-Bromomethanesulfonyl-pyridine CAS 986260). Yield from IM46 = 1.54 g (68%). 1H-NMR (500 MHz, DMSO) 6 8.80 (d,1H), 8.72 (s, 1H), 8.02 (m, 2H), 7.27 (m, 2H), 7.20 (s, 1H), 7.12 (d, 2H), 5.08 (m, 1H), 3.27 [Annotation] sak (s, 3H), 2.20 (m, 1H), 1.95 (m, 1H), 1.42 (d, 3H), 1.37 (m, 1H),1.22 (m, 1H). LC-MS (m/z) 345.1 (MH+), tR = 1.21 min (method A).
Compound 52: (1 S, 2S)-2—Phenyl—cyclopropanecarboxy/ic acid [(R)(5-ethoxy—pyridin- 2-yl)hydroxy—ethy/]—amide MHZ ,. VA 3 NH 0 N . 1"'V)LOH I / OH \ O I Prepared analogously to Compound 21 using IM46 and Amino(5-ethoxy-pyridin hanol (prepared from commercially available 2-Bromoethoxy-pyridine CAS 42834 analogously to lM36). Compound 52 was further purified by SFC (Column: Chiralpack OJ250x30 mm, Mobile phase: Supercrital C02/MeOH+NH4OH = 55/45 at 50 , column temperature: 38°C, Nozzle Pressure: 100 Bar, Nozzle Temp = 60°C, Evaporator temp = 20 C, r temp =25°C, Detector: 220 nm). Yield = 163 mg. 1H NMR (CDCI3 400MHz, TMS): 6 8.15 (d, J = 2.8 Hz, 1 H), 7.08-7.31 (m, 6 H), 7.06 (d, J = 7.2 Hz, 2 H), 5.11-5.15 (m, 1 H), 4.00-4.08 (m, 3 H), 3.87-3.90 (m, 1 H), 2.47-2.52 (m, 1 H), 1.63-1.76 (m, 3 H), 1.43 (t, J = 7.2 Hz, 3 H), 1.27-1.31 (m, 1 H); [01]20,D = 182.0 (c = 0.234 g/100mL, EtOH) In vitro assays The nicotinic acetylcholine or 07 is a calcium-permeable ion channel, whose ac- tivity can be measured by over expression in mammalian cells or oocytes. These two individ- ual assays are described in Example 2 and 3, respectively.
Example 2: 017 NNR flux assay The nicotinic acetylcholine receptor 07 is a m-permeable ion channel, whose ac- tivity can be measured by over expression in mammalian cells or oocytes. In this version of the assay, the human 07 receptor is stably expressed in the rat GH4C1 cell line. The assay was used to identify positive allosteric modulators (PAMs) of the 017 receptor. Activation of the channel was measured by loading cells with the calcium-sensitive fluorescent dye Calcium-4 (Assay kit from Molecular Devices), and then measuring real-time s in fluorescence upon treatment with test compounds.
[Annotation] sak The cell line ChanClone GH4C1-nAChRalpha7 from Genionics was seeded from frozen stock in 384-well plates in culture media 2-3 days before experiment to form an approximately 80% confluent layer on the day of experiment.
Cell plating and dye loading The cell culture were split into “22.5cm x 22.5cm”-plates with imately 100x103 cells/cm2. After four days incubation in a humidified incubator at 37°C and 5% C02, it had grown to an 80-90% confluent layer, and the cells were harvested.
Culture media: 500 mL 12 (Gibco 31331) 50 mL FBS (Gibco 10091-155, lot 453269FD) mL Sodium Pyruvate (Gibco 11360) mL Pen/Strep (Gibco 15140) 0.1 mg/mL Gibco11811-064) Two or three days before the experiment the cells were seeded in 384 well plates from Greiner bio-one (781946, CELLCOAT, Poly-D-Lysine, black, uClear).
The media was poured off and the plate washed with PBS and left to drain. 5 mL Trypsin was added, cells were washed and ted (at room temperature) for about 10 seconds. Tryp- sin was poured of quickly and the cells were incubated for 2 s at 37°C (if the cells were not already detached). Cells were resuspended in 10 mL culture media and transfered to 50 mL tubes.
The cell suspension was counted (NucleoCounter, total cell count) from the first plates to estimate the total cell number of the whole batch.
The cells were seeded in 384 well plates with 30 l (30000 cells/well) while stirring the cell suspension or otherwise preventing the cells from precipitating.
The plates were incubated at room temperature for 30-45 minutes.
The plates were placed in incubator for two days (37°C and 5% C02).
Loading the Cells The loading buffer was 5% v/v Calcium-4 Kit and 2.5 mM Probenecid in assay buffer. 190 mL assay buffer mL lution 2 mL 250 mM Probenecid This volume was enough for 3 x 8 cell plates.
Culture media were removed from the cell plates and 20 uL loading buffer was added in each well. The cell plates were placed in trays and incubated 90 minutes in the incubator [Annotation] sak . Thereafter the plates were incubated 30 minutes at toom temperature, still protected from light.
Now the cell plates were ready to run in the Functional Drug Screening System (FDSS).
The assay buffer was HBSS with 20 mM HEPES, pH 7.4 and 3 mM CaClz.
FDSS Ca assay 200 nL 10 mM compound solution in DMSO was diluted in 50 uL assay buffer. The fi- nal test concentrations in the cell plates were 205-2.5-1.25-0.625-0.312-0.156 0.039 uM. Assay buffer and 3 uM PNU-120596 were used for l.
The agonist acetylcholine was added to a final concentration of 20 uM (~EC100).
In the FDSS7000 the Ex480-Em540 was ed with 1 second intervals. The ne was made of 5 frames before addition of test compounds, and 95 frames more were made before addition of acetylcholine. The measurement d 30 frames after the 2nd addition.
Raw data for each well were collected as “the maximum fluorescence count” in the interval 100-131 seconds and as “the average fluorescence count” in the interval 96-100 seconds.
The positive allosteric modulation in the 2nd addition was the enhancement of agonist re- sponse with test compound compared to agonist alone.
Results were calculated as % modulation of test compound ed to the reference PNU-120596 set to 100%. From these data EC50 curves were generated giving EC50, hill and maximum ation.
The compounds of the invention were shown to be PAMs of the o7 receptor. The com- pounds of the present invention characterized in the flux assay generally possess EC50 val- ues below 20.000 nM or less such as below 10.000 nM. Many compounds, in fact have EC50 values below 5.000 nM. Table 1 shows EC50 values for exemplified compounds of the inven- tion.
Table 1 E0000 E0000 E0000 [Annotation] sak Example 3: a7NNR oocyte assay Expression of (17 nACh ors in Xenopus oocytes.
Oocytes were surgically removed from mature female Xenepus laevis anaesthetized in 0.4% MS—222 for 10 — 15 min. The oocytes were then digested at room temperature for 2-3 hours with 0.5 mg/mL collagenase (type IA Sigma-Aldrich) in 0R2 buffer (82.5 mM NaCl, 2.0 mM KCI, 1.0 mM MgClz and 5.0 mM HEPES, pH 7.6). Oocytes avoid of the follicle layer were selected and incubated for 24 hours in Modified Barth's Saline buffer (88 mM NaCl, 1 mM KCI, 15 mM HEPES, 2.4 mM NaHCOs, 0.41 mM CaClz, 0.82 mM MgSO4, 0.3 mM Ca(NOs)2) supplemented with 2 mM sodium pyruvate, 0.1 U/l llin and 0.1 ug/l streptomycin. Stage IV oocytes were identified and ed with 4.2 - 48 hi of nuclease free water ning 0.1 — 1.2 ng of cRNA coding for human (17 nACh receptors or 3.0 — 32 ng of cRNA coding for rat o7 nACh receptors and incubated at 18°C for 1 — 10 days when they were used for electro- physiological recordings.
Electrophysiological ings of (17 nACh receptors sed in oocytes.
Oocytes were used for electrophysiological recordings 1 — 10 days after injection.
Oocytes were placed in a 1 mL bath and perfused with Ringer buffer (115 mM NaCl, 2.5 mM KCI, 10 mM HEPES, 1.8 mM CaClz, 0.1 mM MgClz, pH 7.5). Cells were impaled with agar plugged 0.2 — 1 M0 odes containing 3 M KCI and voltage clamped at -90 mV by a Ge- neClamp 500B amplifier. The experiments were performed at room temperature. Oocytes were continuously perfused with Ringer buffer and the drugs were applied in the perfusate.
ACh (30 uM) applied for 30 sec were used as the standard agonist for activation of the (17 [Annotation] sak nACh receptors. In the standard screening set-up the new test compound (10 uM or 30 uM) were applied for 1 min of pre-application allowing for evaluation of agonistic ty followed by 30 sec of co-application with ACh (30 uM) allowing for evaluation of PAM ty. The re- sponse of co-application was compared to the agonistic response obtained with ACh alone.
The drug induced effects on both the peak response and the total charge (AUC) response were calculated thus giving the effect of drug induced PAM ty as fold modulation of the control response.
For more elaborate studies doses-response curves can be performed for evaluation of max-fold modulation and E050 values for both peak and AUC responses.
WE

Claims (14)

CLAIM :
1. A nd according to formula [I] R3 R5 R6 R2 N A R1 A R11 9 wherein R1, R2, R3, R4 and R5 are H; R6 is methoxymethyl; A7 is C-R7, A8 is N and A9 is C-R9; R7, R9, R10 and R11 are selected ndently of each other from H, C1-6alkyl, C2-6alkenyl, C2-6alkynyl, C1-6alkoxy and OR12; wherein R12 represents a monocyclic saturated ring moiety having 4-6 ring atoms wherein one of said ring atoms is O and the rest is C; and ceutically acceptable salts thereof.
2. The nd according to claim 1, wherein R7, R10 and R11 all represent H.
3. The compound according to claim 1 or 2, wherein R9 is C1-4alkoxy.
4. The compound according to claim 1 or 2, wherein R9 is OR12, wherein R12 represents a monocyclic saturated ring moiety having 4-6 ring atoms wherein one of said ring atoms is O and the rest is C.
5. The compound according to any one of claims 1-4 having a diastereomeric excess of at least 80%, such as at least 85%, such as at least 90%, such as at least 95%.
6. The compound according to claim 1selected from 46: (1S,2S)Phenyl-cyclopropanecarboxylic acid [(R)(6-ethoxy-pyridinyl)methoxy- ethyl]-amide; 47: (1S,2S)-N-[(1R)methoxy[6-[(3R)-tetrahydrofuranyl]oxypyridyl]ethyl]phenyl- cyclopropanecarboxamide; 48: (1S,2S)-N-[(1R)methoxy[6-[(3S)-tetrahydrofuranyl]oxypyridyl]ethyl]phenyl- cyclopropanecarboxamide; 49: (1S,2S)Phenyl-cyclopropanecarboxylic acid {(R)methoxy[6-(tetrahydro-pyran yloxy)-pyridinyl]-ethyl}-amide; and pharmaceutically acceptable salts of any of these compounds.
7. The compound according to claim 1, which is 46: (1S,2S)Phenyl-cyclopropanecarboxylic acid -(6-ethoxy-pyridinyl)methoxy- -amide; and pharmaceutically acceptable salts thereof.
8. The compound according to claim 15, which is 47: )-N-[(1R)methoxy[6-[(3R)-tetrahydrofuranyl]oxypyridyl]ethyl]phenyl- cyclopropanecarboxamide; and pharmaceutically acceptable salts thereof.
9. The compound according to claim 1, which is 48: (1S,2S)-N-[(1R)methoxy[6-[(3S)-tetrahydrofuranyl]oxypyridyl]ethyl]phenyl- cyclopropanecarboxamide; and pharmaceutically acceptable salts thereof.
10. The compound according to claim 1, which is 49: (1S,2S)Phenyl-cyclopropanecarboxylic acid {(R)methoxy[6-(tetrahydro-pyran yloxy)-pyridinyl]-ethyl}-amide; and pharmaceutically acceptable salts f.
11. A compound according to any one of claims 1-10, for use as a medicament.
12. A compound according to any one of claims 1-10, for use in the ent of a disease or disorder selected from psychosis; schizophrenia; cognitive disorders; cognitive ment associated with schizophrenia; attention deficit hyperactivity disorder (ADHD); au- tism spectrum disorders, Alzheimer’s disease (AD); mild cognitive impairment (MCI); age associated memory impairment (AAMI); senile ia; AIDS dementia; Pick's disease; dementia associated with Lewy bodies; dementia associated with Down's syndrome; Huntington's disease; Parkinson’s disease (PD); obsessive-compulsive disorder (OCD); tic brain injury; epilepsy; post-traumatic stress; Wernicke-Korsakoff syndrome (WKS); post- traumatic amnesia; cognitive deficits associated with depression; diabetes, weight control, inflammatory disorders, reduced angiogenesis; amyotrophic lateral sclerosis and pain.
13. A pharmaceutical composition comprising a compound according to any of claims 1- 10, and one or more pharmaceutically acceptable carrier or ent.
14. A kit comprising a nd according to any of claims 1-10, er with a second compound selected from the list consisting of acetylcholinesterase inhibitors; glutamate receptor antagonists; dopamine transport inhibitors; noradrenalin transport inhibitors; D2 antagonists ; D2 partial ts; PDE10 antagonists; 5-HT2A antagonists; 5-HT6 antagonists; KCNQ antagonists; lithium; sodium l blockers and GABA signaling enhancers. H. Lundbeck A/S By the Attorneys for the Applicant SPRUSON & FERGUSON Per:
NZ710003A 2011-07-08 2012-07-06 Positive allosteric modulators of nicotinic acetylcholine receptor NZ710003B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA201100520 2011-07-08
DKPA201100520 2011-07-08
NZ617367A NZ617367B2 (en) 2011-07-08 2012-07-06 Positive allosteric modulators of nicotinic acetylcholine receptor

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NZ710003A NZ710003A (en) 2017-01-27
NZ710003B2 true NZ710003B2 (en) 2017-04-28

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