NZ606397B - Spinach hybrid rx 06682141 - Google Patents
Spinach hybrid rx 06682141Info
- Publication number
- NZ606397B NZ606397B NZ606397A NZ60639713A NZ606397B NZ 606397 B NZ606397 B NZ 606397B NZ 606397 A NZ606397 A NZ 606397A NZ 60639713 A NZ60639713 A NZ 60639713A NZ 606397 B NZ606397 B NZ 606397B
- Authority
- NZ
- New Zealand
- Prior art keywords
- plant
- spinach
- hybrid
- seed
- plants
- Prior art date
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Abstract
606397 Disclosed is a seed of spinach hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505. Also disclosed is a method for producing a seed of a hybrid RX 06682141-derived spinach plant comprising the steps of: (a) crossing a spinach plant of hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505, with a second spinach plant; and (b) allowing seed of a RX 06682141-derived spinach plant to form. t of hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505, with a second spinach plant; and (b) allowing seed of a RX 06682141-derived spinach plant to form.
Description
NEW ZEALAND
Patents Act 1953
Patents Form No. 5
COMPLETE SPECIFICATION
Title:
SPINACH HYBRID RX 06682141
We, Seminis Vegetable Seeds, Inc., of 800 N. Lindbergh Blvd., St. Louis, MO 63167,
United States of America (United States), do hereby declare the invention for which we
pray that a patent may be granted to us and the method by which it is to be performed, to
be particularly described in and by the following statement.
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims the priority of U.S. Provisional Appl. Ser. No.
61/596,434, filed February 8, 2012, and U.S. Application Ser. No. US 13/419,251, filed 13
March 2012, the entire disclosures of which are incorporated herein by reference.
FIELD OF THE INVENTION
The present invention relates to the field of plant breeding and, more specifically,
to the development of spinach hybrid RX 06682141, and parent spinach lines LSA 66-1041F and
SSB 66-1131 M.
BACKGROUND OF THE INVENTION
The goal of vegetable breeding is to combine various desirable traits in a single
variety/hybrid. Such desirable traits may include any trait deemed beneficial by a grower and/or
consumer, including greater yield, resistance to insects or pests, tolerance to environmental
stress, better agronomic quality, higher nutritional value, growth rate and fruit properties.
Breeding techniques take advantage of a plant’s method of pollination. There are
two general methods of pollination: a plant self-pollinates if pollen from one flower is transferred
to the same or another flower of the same plant or plant variety. A plant cross-pollinates if
pollen comes to it from a flower of a different plant variety.
Plants that have been self-pollinated and selected for type over many generations
become homozygous at almost all gene loci and produce a uniform population of true breeding
progeny, a homozygous plant. A cross between two such homozygous plants of different
genotypes produces a uniform population of hybrid plants that are heterozygous for many gene
loci. Conversely, a cross of two plants each heterozygous at a number of loci produces a
population of hybrid plants that differ genetically and are not uniform. The resulting non-
uniformity makes performance unpredictable.
The development of uniform varieties requires the development of homozygous
inbred plants, the crossing of these inbred plants, and the evaluation of the crosses. Pedigree
breeding and recurrent selection are examples of breeding methods that have been used to
develop inbred plants from breeding populations. Those breeding methods combine the genetic
backgrounds from two or more plants or various other broad-based sources into breeding pools
from which new lines and hybrids derived therefrom are developed by selfing and selection of
desired phenotypes. The new lines and hybrids are evaluated to determine which of those have
commercial potential.
SUMMARY OF THE INVENTION
In one aspect, the present invention provides a spinach plant of the hybrid
designated RX 06682141, the spinach line LSA 66-1041F, or SSB 66-1131 M. Also provided
are spinach plants having all the physiological and morphological characteristics of such a plant.
Parts of these spinach plants are also provided, for example, including pollen, an ovule, scion, a
rootstock, a fruit, and a cell of the plant.
In another aspect of the invention, a plant of spinach hybrid RX 06682141 and/or
spinach lines LSA 66-1041F and SSB 66-1131 M comprising an added heritable trait is
provided. The heritable trait may comprise a genetic locus that is, for example, a dominant or
recessive allele. In one embodiment of the invention, a plant of spinach hybrid RX 06682141
and/or spinach lines LSA 66-1041F and SSB 66-1131 M is defined as comprising a single locus
conversion. In specific embodiments of the invention, an added genetic locus confers one or
more traits such as, for example, herbicide tolerance, insect resistance, disease resistance, and
modified carbohydrate metabolism. In further embodiments, the trait may be conferred by a
naturally occurring gene introduced into the genome of a line by backcrossing, a natural or
induced mutation, or a transgene introduced through genetic transformation techniques into the
plant or a progenitor of any previous generation thereof. When introduced through
transformation, a genetic locus may comprise one or more genes integrated at a single
chromosomal location.
The invention also concerns the seed of spinach hybrid RX 06682141 and/or
spinach lines LSA 66-1041F and SSB 66-1131 M. The spinach seed of the invention may be
provided as an essentially homogeneous population of spinach seed of spinach hybrid RX
06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131 M. Essentially homogeneous
populations of seed are generally free from substantial numbers of other seed. Therefore, seed of
hybrid RX 06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131 M may be defined as
forming at least about 97% of the total seed, including at least about 98%, 99% or more of the
seed. The seed population may be separately grown to provide an essentially homogeneous
population of spinach plants designated RX 06682141 and/or spinach lines LSA 66-1041F and
SSB 66-1131 M.
In yet another aspect of the invention, a tissue culture of regenerable cells of a
spinach plant of hybrid RX 06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131 M
is provided. The tissue culture will preferably be capable of regenerating spinach plants capable
of expressing all of the physiological and morphological characteristics of the starting plant, and
of regenerating plants having substantially the same genotype as the starting plant. Examples of
some of the physiological and morphological characteristics of the hybrid RX 06682141 and/or
spinach lines LSA 66-1041F and SSB 66-1131 M include those traits set forth in the tables
herein. The regenerable cells in such tissue cultures may be derived, for example, from embryos,
meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistils, flowers, seed and stalks.
Still further, the present invention provides spinach plants regenerated from a tissue culture of
the invention, the plants having all the physiological and morphological characteristics of hybrid
RX 06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131 M.
In still yet another aspect of the invention, processes are provided for producing
spinach seeds, plants and fruit, which processes generally comprise crossing a first parent
spinach plant with a second parent spinach plant, wherein at least one of the first or second
parent spinach plants is a plant of spinach line LSA 66-1041F or SSB 66-1131 M. These
processes may be further exemplified as processes for preparing hybrid spinach seed or plants,
wherein a first spinach plant is crossed with a second spinach plant of a different, distinct
genotype to provide a hybrid that has, as one of its parents, a plant of spinach line LSA 66-1041F
or SSB 66-1131 M. In these processes, crossing will result in the production of seed. The seed
production occurs regardless of whether the seed is collected or not.
In one embodiment of the invention, the first step in “crossing” comprises
planting seeds of a first and second parent spinach plant, often in proximity so that pollination
will occur for example, mediated by insect vectors. Alternatively, pollen can be transferred
manually. Where the plant is self-pollinated, pollination may occur without the need for direct
human intervention other than plant cultivation.
A second step may comprise cultivating or growing the seeds of first and second
parent spinach plants into plants that bear flowers. A third step may comprise preventing self-
pollination of the plants, such as by emasculating the flowers (i.e., killing or removing the
pollen).
A fourth step for a hybrid cross may comprise cross-pollination between the first
and second parent spinach plants. Yet another step comprises harvesting the seeds from at least
one of the parent spinach plants. The harvested seed can be grown to produce a spinach plant or
hybrid spinach plant.
The present invention also provides the spinach seeds and plants produced by a
process that comprises crossing a first parent spinach plant with a second parent spinach plant,
wherein at least one of the first or second parent spinach plants is a plant of spinach hybrid RX
06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131 M. In one embodiment of the
invention, spinach seed and plants produced by the process are first generation (F ) hybrid
spinach seed and plants produced by crossing a plant in accordance with the invention with
another, distinct plant. The present invention further contemplates plant parts of such an F
hybrid spinach plant, and methods of use thereof. Therefore, certain exemplary embodiments of
the invention provide an F hybrid spinach plant and seed thereof.
In still yet another aspect, the present invention provides a method of producing a
plant derived from hybrid RX 06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131
M, the method comprising the steps of: (a) preparing a progeny plant derived from hybrid RX
06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131 M, wherein said preparing
comprises crossing a plant of the hybrid RX 06682141 and/or spinach lines LSA 66-1041F and
SSB 66-1131 M with a second plant; and (b) crossing the progeny plant with itself or a second
plant to produce a seed of a progeny plant of a subsequent generation. In further embodiments,
the method may additionally comprise: (c) growing a progeny plant of a subsequent generation
from said seed of a progeny plant of a subsequent generation and crossing the progeny plant of a
subsequent generation with itself or a second plant; and repeating the steps for an additional 3-10
generations to produce a plant derived from hybrid RX 06682141 and/or spinach lines LSA 66-
1041F and SSB 66-1131 M. The plant derived from hybrid RX 06682141 and/or spinach lines
LSA 66-1041F and SSB 66-1131 M may be an inbred line, and the aforementioned repeated
crossing steps may be defined as comprising sufficient inbreeding to produce the inbred line. In
the method, it may be desirable to select particular plants resulting from step (c) for continued
crossing according to steps (b) and (c). By selecting plants having one or more desirable traits, a
plant derived from hybrid RX 06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131
M is obtained which possesses some of the desirable traits of the line/hybrid as well as
potentially other selected traits.
In certain embodiments, the present invention provides a method of producing
food or feed comprising: (a) obtaining a plant of spinach hybrid RX 06682141 and/or spinach
lines LSA 66-1041F and SSB 66-1131 M, wherein the plant has been cultivated to maturity, and
(b) collecting at least one spinach from the plant.
In still yet another aspect of the invention, the genetic complement of spinach
hybrid RX 06682141 and/or spinach lines LSA 66-1041F and SSB 66-1131 M is provided. The
phrase “genetic complement” is used to refer to the aggregate of nucleotide sequences, the
expression of which sequences defines the phenotype of, in the present case, a spinach plant, or a
cell or tissue of that plant. A genetic complement thus represents the genetic makeup of a cell,
tissue or plant, and a hybrid genetic complement represents the genetic make up of a hybrid cell,
tissue or plant. The invention thus provides spinach plant cells that have a genetic complement
in accordance with the spinach plant cells disclosed herein, and seeds and plants containing such
cells.
Plant genetic complements may be assessed by genetic marker profiles, and by
the expression of phenotypic traits that are characteristic of the expression of the genetic
complement, e.g., isozyme typing profiles. It is understood that hybrid RX 06682141 and/or
spinach lines LSA 66-1041F and SSB 66-1131 M could be identified by any of the many well
known techniques such as, for example, Simple Sequence Length Polymorphisms (SSLPs)
(Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990), Randomly Amplified Polymorphic
DNAs (RAPDs), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified
Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified
Fragment Length Polymorphisms (AFLPs) (EP 534 858, specifically incorporated herein by
reference in its entirety), and Single Nucleotide Polymorphisms (SNPs) (Wang et al., Science,
280:1077-1082, 1998).
In still yet another aspect, the present invention provides hybrid genetic
complements, as represented by spinach plant cells, tissues, plants, and seeds, formed by the
combination of a haploid genetic complement of a spinach plant of the invention with a haploid
genetic complement of a second spinach plant, preferably, another, distinct spinach plant. In
another aspect, the present invention provides a spinach plant regenerated from a tissue culture
that comprises a hybrid genetic complement of this invention.
Any embodiment discussed herein with respect to one aspect of the invention
applies to other aspects of the invention as well, unless specifically noted.
The term “about” is used to indicate that a value includes the standard deviation
of the mean for the device or method being employed to determine the value. The use of the
term “or” in the claims is used to mean “and/or” unless explicitly indicated to refer to
alternatives only or the alternatives are mutually exclusive. When used in conjunction with the
word “comprising” or other open language in the claims, the words “a” and “an” denote “one or
more,” unless specifically noted otherwise. The terms “comprise,” “have” and “include” are
open-ended linking verbs. Any forms or tenses of one or more of these verbs, such as
“comprises,” “comprising,” “has,” “having,” “includes” and “including,” are also open-ended.
For example, any method that “comprises,” “has” or “includes” one or more steps is not limited
to possessing only those one or more steps and also covers other unlisted steps. Similarly, any
plant that “comprises,” “has” or “includes” one or more traits is not limited to possessing only
those one or more traits and covers other unlisted traits.
Other objects, features and advantages of the present invention will become
apparent from the following detailed description. It should be understood, however, that the
detailed description and any specific examples provided, while indicating specific embodiments
of the invention, are given by way of illustration only, since various changes and modifications
within the spirit and scope of the invention will become apparent to those skilled in the art from
this detailed description.
DETAILED DESCRIPTION OF THE INVENTION
The invention provides methods and compositions relating to plants, seeds and
derivatives of spinach hybrid RX 06682141, spinach line LSA 66-1041F or SSB 66-1131 M.
Spinach (Spinacia oleracea L.) line SSB66-1131 M exhibits a number of
beneficial traits including resistance to Peronospora farinosa f.sp. spinaciae against the races Pfs
1 till Pfs 13.
Spinach (Spinacia oleracea L.) hybrid RX 06682141 is a late bolting, Savoy leaf-
type variety, exhibiting resistance against Peronospora farinosa f.sp. spinaciae races Pfs 1 till
Pfs 13.
A. Origin and Breeding History of Spinach Hybrid RX 06682141
RX 06682141 was developed at the Monsanto Research station in Wageningen,
The Netherlands, initially by crossing line LSA66-1041F as a female parent with SSB66-1131 M
as a male parent. Observation during the variety selections over three years confirmed that RX
06682141 is uniform and stable. As is true with other spinach varieties, a small percentage of
off-types can occur for almost any characteristics during the course of repeated multiplications.
However, no variants were observed for RX 06682141. It is uniform and stable.
Line SSB66-1131 M was developed at the Monsanto Research station in
Wageningen, The Netherlands, by pedigree selection out of a breeding cross between
F1[Trentino] Pop Vriend and inbred line SSB66-8M. The origin and selections that led to the
development of SSB66-1131 M can be summarized as follows (S = Selfing, M=Mass selection):
Year Generation Material Main selection criteria
Year 1 F1 [Trentino] * SSB66-0008M Making breeding cross
Year 2 F2 Select for Pf resistance and
plant type
Year 3 F2.S1 Select for plant type (bolting)
Year 3 F2.S2 Uniformity
Year 6 F2,S2.M1 Uniformity
The final outcome is a line, which combines a late bolting plant type with
resistances against the Downey mildew races Pfs 1 till Pfs 13. Observation during the mass
selection one year and stock seed production another year confirmed that SSB66-1131 M is
uniform and stable. As is true with other spinach varieties, a small percentage of off-types can
occur for almost any characteristics during the course of repeated multiplications. However, no
variants were observed during the two years in which SSB66-1131 M was observed to be
uniform and stable.
The parent lines are uniform and stable, as is a hybrid produced therefrom. A
small percentage of variants can occur within commercially acceptable limits for almost any
characteristic during the course of repeated multiplication. However no variants are expected.
B. Physiological and Morphological Characteristics of Spinach Hybrid RX
06682141, and Spinach Lines LSA 66-1041F and SSB 66-1131 M
In accordance with one aspect of the present invention, there is provided a plant
having the physiological and morphological characteristics of spinach hybrid RX 06682141 and
the parent lines thereof. A description of the physiological and morphological characteristics of
such plants is presented in Tables 1-2.
Table 1: Physiological and Morphological Characteristics of Hybrid RX 06682141
CHARACTERISTIC RX 06682141 Santorini
Ploidy diploid diploid
Maturity
medium (Long Standing medium (Long Standing
Growth Rate
Bloomsdale) Bloomsdale)
Days from planting to prime
45 45
market stage
Plant (Prime Market Stage)
semi-erect (Long Standing semi-erect (Long Standing
Habit
Bloomsdale) Bloomsdale)
Size medium medium
Spread (cm) 32.3 cm 34.7 cm
Height (cm) 11 cm 14 cm
Seedling Cotyledon
Length of cotyledon medium medium
Width (mm) 5.6 mm 5.7 mm
Length (mm) 59 mm 64 mm
Tip pointed pointed
Color medium green medium green
Color Chart Name RHS
CHARACTERISTIC RX 06682141 Santorini
Color Chart Value 146B 146B
Leaf (First Foliage Leaves)
Shape circular ovate
Base straight straight
Tip round round
Margin slightly curled slightly curled
Upper Surface Color medium green (Giant Nobel) medium green (Giant Nobel)
Color Chart Name RHS
Color Chart Value 146A 146A
Lower Surface Color
(Compared with upper lighter lighter
surface)
Color Chart Name RHS
Color Chart Value 146B 146B
Leaf (Prime Market Stage)
Surface savoy (Virginia Savoy) savoy (Virginia Savoy)
Base lobed lobed
Tip round round
Margin curled under curled under
dark green (Standing
Upper Surface Color medium green (Giant Nobel)
Bloomsdale)
Color Chart Name RHS
Color Chart Value 147A 146A
Lower Surface Color
(Compared with upper lighter lighter
surface)
Color Chart Name RHS
CHARACTERISTIC RX 06682141 Santorini
Color Chart Value 147B 146B
Luster glossy glossy
Blade size medium (Virginia Savoy) medium (Virginia Savoy)
Blade Lobing weak [Butterflay, Giraffe]
Blade Attitude (observed in
relation to the horizontal, semi-pendulous [Giraffe, semi-pendulous [Giraffe,
independent of the attitude of Medania] Medania]
the petiole)
Blade Shape [excluding basal
broad ovate broad ovate
lobes] (prime market stage)
Blade curving of margin recurved (Imola) recurved (Imola)
Blade shape of apex rounded (Imola, Nores) rounded (Imola, Nores)
Blade shape in longitudinal
convex (Grappa, Lazio) convex (Grappa, Lazio)
section
Petiole Attitude semi-erect [Monnopa, Parrot] semi-erect [Monnopa, Parrot]
Petiole Length (prime market medium [TG=Butterflay,
short [TG = Imola, Mystic]
stage) Giraffe]
Petiole Color medium green medium green
Color Chart Name RHS
Color Chart Value 143A 143A
Petiole Red Pigmentation absent absent
Petiole Length to the Blade 6 cm 10 cm
Petiole Diameter (mm) 7 mm 7 mm
Petiole Diameter medium medium
Seed Stalk Development
Start of Bolting (10% of
late (Norgreen) late (Norgreen)
plants)
Height of Stalk (cm) 92.1 cm 96.4 cm
CHARACTERISTIC RX 06682141 Santorini
Leaves on Stalk of Female
few or none few or none
Plant
Leaves on Stalk of Male
few or none few or none
Plant
Plants that are Female 0-10% [Monnopa] 0-10% [Monnopa]
Plants that are Male 0-10% [Monnopa, Parrot] 0-10% [Monnopa, Parrot]
Plants that are Monoecious 91-100% [Monnopa] 91-100% [Monnopa]
Seed
Surface smooth smooth
Spines (harvested seed) absent [Resistoflay] absent [Resistoflay]
*These are typical values. Values may vary due to environment. Other values that are
substantially equivalent are also within the scope of the invention.
Table 2: Physiological and Morphological Characteristics of Line SSB 66-1131 M
Comparison Variety:
CHARACTERISTIC SSB 66-1131M
SSB 66-1061M
Ploidy
diploid diploid
Maturity
medium (Long Standing
Growth Rate slow (Norgreen)
Bloomsdale)
Days from planting to prime
47 44
market stage
Plant (Prime Market Stage)
Habit flat (Viroflay) flat (Viroflay)
Size small (America) medium
Spread (cm) 35 cm 45 cm
Height (cm) 8 cm 14 cm
Seedling Cotyledon
Comparison Variety:
CHARACTERISTIC SSB 66-1131M
SSB 66-1061M
Length of cotyledon short [Nores] medium
Width (mm) 4.3 mm 4.7 mm
Length (mm) 35 mm 57 mm
Tip pointed pointed
Color dark green medium green
Color Chart Name RHS
Color Chart Value 146A 146B
Leaf (First Foliage Leaves)
Shape ovate ovate
Base straight straight
Tip round-pointed round-pointed
Margin slightly curled slightly curled
Upper Surface Color medium green (Giant Nobel) medium green (Giant Nobel)
Color Chart Name RHS
Color Chart Value 147A 147A
Lower Surface Color
(Compared with upper lighter lighter
surface)
Color Chart Name RHS
Color Chart Value 147B 147B
Leaf (Prime Market Stage)
Surface smooth (Viroflay) smooth (Viroflay)
Base lobed lobed
Tip round round
Margin curled under slightly curved
Comparison Variety:
CHARACTERISTIC SSB 66-1131M
SSB 66-1061M
Upper Surface Color medium green (Giant Nobel) medium green (Giant Nobel)
Color Chart Name RHS
Color Chart Value 147A 146A
Lower Surface Color
(Compared with upper lighter lighter
surface)
Color Chart Name RHS
Color Chart Value 146A 146B
Luster dull glossy
small (Long Standing
Blade size medium (Virginia Savoy)
Bloomsdale)
medium [Butterflay, Koala,
Blade Blistering weak [Polka, Tarpy]
Mystic]
absent or very weak (US = absent or very weak (US =
Blade Lobing
not lobed) not lobed)
semi-erect [Grappa,
Blade Attitude horizontal [Lavewa, Mystic]
Monnopa]
Blade Shape [excluding basal
broad ovate broad ovate
lobes] (prime market stage)
Blade curving of margin recurved (Imola) recurved (Imola)
Blade shape of apex rounded (Imola, Nores) rounded (Imola, Nores)
Blade shape in longitudinal
flat (Mystic, Resistoflay) flat (Mystic, Resistoflay)
section
Petiole Attitude horizontal [Comte, Lavewa] horizontal [Comte, Lavewa]
Petiole Length (prime market medium [TG=Butterflay,
short [TG=Imola, Mystic]
stage) Giraffe]
Petiole Color light green light green
Color Chart Name RHS
Comparison Variety:
CHARACTERISTIC SSB 66-1131M
SSB 66-1061M
Color Chart Value 143A 143A
Petiole Red Pigmentation absent absent
Petiole Length to the Blade 4 cm 9 cm
Petiole Diameter (mm) 6 mm 7 mm
Petiole Diameter medium medium
Seed Stalk Development
Start of Bolting (10% of
late (Norgreen) late (Norgreen)
plants)
Time of start of bolting (for
spring sown crop, 15% of very late [Chico, Lavewa] very late [Chico, Lavewa]
plants)
Height of Stalk (cm) 48 cm 56 cm
Leaves on Stalk of Female
many
Plant
Leaves on Stalk of Male
many many
Plant
Plants that are Female 0-10% [Monnopa] 0-10% [Monnopa]
Plants that are Male 0-10% [Monnopa, Parrot] 0-10% [Monnopa, Parrot]
Plants that are Monoecious 91-100% [Monnopa] 91-100% [Monnopa]
Seed
Surface smooth smooth
Spines (harvested seed) absent [Resistoflay] absent [Resistoflay]
*These are typical values. Values may vary due to environment. Other values that are
substantially equivalent are also within the scope of the invention.
C. Breeding Spinach Plants
One aspect of the current invention concerns methods for producing seed of
spinach hybrid RX 06682141 involving crossing spinach lines LSA 66-1041F and SSB 66-1131
M. Alternatively, in other embodiments of the invention, hybrid RX 06682141, line LSA 66-
1041F or line SSB 66-1131 M may be crossed with itself or with any second plant. Such
methods can be used for propagation of hybrid RX 06682141 and/or the spinach lines LSA 66-
1041F and SSB 66-1131 M, , or can be used to produce plants that are derived from hybrid RX
06682141 and/or the spinach lines LSA 66-1041F and SSB 66-1131 M. Plants derived from
hybrid RX 06682141 and/or the spinach lines LSA 66-1041F and SSB 66-1131 M may be used,
in certain embodiments, for the development of new spinach varieties.
The development of new varieties using one or more starting varieties is well
known in the art. In accordance with the invention, novel varieties may be created by crossing
hybrid RX 06682141 followed by multiple generations of breeding according to such well
known methods. New varieties may be created by crossing with any second plant. In selecting
such a second plant to cross for the purpose of developing novel lines, it may be desired to
choose those plants which either themselves exhibit one or more selected desirable
characteristics or which exhibit the desired characteristic(s) when in hybrid combination. Once
initial crosses have been made, inbreeding and selection take place to produce new varieties. For
development of a uniform line, often five or more generations of selfing and selection are
involved.
Uniform lines of new varieties may also be developed by way of double-haploids.
This technique allows the creation of true breeding lines without the need for multiple
generations of selfing and selection. In this manner true breeding lines can be produced in as
little as one generation. Haploid embryos may be produced from microspores, pollen, anther
cultures, or ovary cultures. The haploid embryos may then be doubled autonomously, or by
chemical treatments (e.g. colchicine treatment). Alternatively, haploid embryos may be grown
into haploid plants and treated to induce chromosome doubling. In either case, fertile
homozygous plants are obtained. In accordance with the invention, any of such techniques may
be used in connection with a plant of the invention and progeny thereof to achieve a homozygous
line.
Backcrossing can also be used to improve an inbred plant. Backcrossing
transfers a specific desirable trait from one inbred or non-inbred source to an inbred that lacks
that trait. This can be accomplished, for example, by first crossing a superior inbred (A)
(recurrent parent) to a donor inbred (non-recurrent parent), which carries the appropriate locus or
loci for the trait in question. The progeny of this cross are then mated back to the superior
recurrent parent (A) followed by selection in the resultant progeny for the desired trait to be
transferred from the non-recurrent parent. After five or more backcross generations with
selection for the desired trait, the progeny have the characteristic being transferred, but are like
the superior parent for most or almost all other loci. The last backcross generation would be
selfed to give pure breeding progeny for the trait being transferred.
The plants of the present invention are particularly well suited for the
development of new lines based on the elite nature of the genetic background of the plants. In
selecting a second plant to cross with RX 06682141 and/or spinach lines LSA 66-1041F and
SSB 66-1131 M for the purpose of developing novel spinach lines, it will typically be preferred
to choose those plants which either themselves exhibit one or more selected desirable
characteristics or which exhibit the desired characteristic(s) when in hybrid combination.
Examples of desirable traits may include, in specific embodiments, high seed yield, high seed
germination, seedling vigor, high fruit yield, disease tolerance or resistance, and adaptability for
soil and climate conditions. Consumer-driven traits, such as a fruit shape, color, texture, and
taste are other examples of traits that may be incorporated into new lines of spinach plants
developed by this invention.
D. Performance Characteristics
As described above, hybrid RX 06682141 exhibits desirable traits, as conferred
by spinach lines LSA 66-1041F and SSB 66-1131 M. The performance characteristics of hybrid
RX 06682141 and spinach lines LSA 66-1041F and SSB 66-1131 M were the subject of an
objective analysis of the performance traits relative to other varieties. The results of the analysis
are presented below.
Spinach line SSB66-1131 M can be characterized as a late bolting line that has
spineless seeds (smooth) and with a resistance to downy mildew (Peronospora farinosa f.sp.
spinaciae (Pfs) races Pfs 1 till Pfs 13. Furthermore the line is phenotypically distinct from all
well-known material by a combination of dark and small leafs.
The parental line believed to most closely resemble SSB66-1131 M is Monsanto
parental line SSB66-1061M. Comparative characteristics distinguish the two lines that include,
but may not be limited to resistances, leaf shape, leaf color and seed stalk length. The candidate
line SSB66-1131 M has other more resistances, has rounder leaf shape, has got darker color and
shorter seed stalk length compared to SMB66-1061M.
Hybrid RX 06682141 can be characterized as a late bolting hybrid that has
spineless seeds (smooth) with a resistance to downy mildew (Peronospora farinosa f.sp.
spinaciae (Pfs) races Pfs 1 till Pfs 13. Furthermore the hybrid is phenotypically distinct from all
well-known material by a combination of almost round leafs with strong Savoynes.
To our knowledge, the hybrid that most closely resembles RX 06682141 is
Monsanto variety Santorini. The comparative characteristics that mostly distinguish to two
hybrids include, but may not be limited to resistances, blistering and leaf color. The candidate
variety RX 06682141 a different resistance pattern, less blistering and darker leaves compared to
Santorini.
Table 3: Performance Characteristics For Line SSB66-1131 M and Comparative Line SSB66-1061M
Material Leaf color Leaf shape Seed stalk length Resistances Pfs
(cm)
SSB66-1061M Medium dark Ovate to 56 1 until 7,9,11,13
round
SSB66-1131 M dark Round 48 1 until 13
Table 4: Comparison of Hybrid RX 06682141 with Santorini (Location: Wageningen, the Netherlands.)
Resistances Blistering Leaf Color
RX 06682141 Pfs 1-13 Less Darker
Santorini Pfs 1-7,9,11,13 More Lighter
E. Further Embodiments of the Invention
In certain aspects of the invention, plants described herein are provided modified
to include at least a first desired heritable trait. Such plants may, in one embodiment, be
developed by a plant breeding technique called backcrossing, wherein essentially all of the
morphological and physiological characteristics of a variety are recovered in addition to a
genetic locus transferred into the plant via the backcrossing technique. The term single locus
converted plant as used herein refers to those spinach plants which are developed by a plant
breeding technique called backcrossing, wherein essentially all of the morphological and
physiological characteristics of a variety are recovered in addition to the single locus transferred
into the variety via the backcrossing technique. By essentially all of the morphological and
physiological characteristics, it is meant that the characteristics of a plant are recovered that are
otherwise present when compared in the same environment, other than an occasional variant trait
that might arise during backcrossing or direct introduction of a transgene.
Backcrossing methods can be used with the present invention to improve or
introduce a characteristic into the present variety. The parental spinach plant which contributes
the locus for the desired characteristic is termed the nonrecurrent or donor parent. This
terminology refers to the fact that the nonrecurrent parent is used one time in the backcross
protocol and therefore does not recur. The parental spinach plant to which the locus or loci from
the nonrecurrent parent are transferred is known as the recurrent parent as it is used for several
rounds in the backcrossing protocol.
In a typical backcross protocol, the original variety of interest (recurrent parent) is
crossed to a second variety (nonrecurrent parent) that carries the single locus of interest to be
transferred. The resulting progeny from this cross are then crossed again to the recurrent parent
and the process is repeated until a spinach plant is obtained wherein essentially all of the
morphological and physiological characteristics of the recurrent parent are recovered in the
converted plant, in addition to the single transferred locus from the nonrecurrent parent.
The selection of a suitable recurrent parent is an important step for a successful
backcrossing procedure. The goal of a backcross protocol is to alter or substitute a single trait or
characteristic in the original variety. To accomplish this, a single locus of the recurrent variety is
modified or substituted with the desired locus from the nonrecurrent parent, while retaining
essentially all of the rest of the desired genetic, and therefore the desired physiological and
morphological constitution of the original variety. The choice of the particular nonrecurrent
parent will depend on the purpose of the backcross; one of the major purposes is to add some
commercially desirable trait to the plant. The exact backcrossing protocol will depend on the
characteristic or trait being altered and the genetic distance between the recurrent and
nonrecurrent parents. Although backcrossing methods are simplified when the characteristic
being transferred is a dominant allele, a recessive allele, or an additive allele (between recessive
and dominant), may also be transferred. In this instance it may be necessary to introduce a test
of the progeny to determine if the desired characteristic has been successfully transferred.
In one embodiment, progeny spinach plants of a backcross in which a plant
described herein is the recurrent parent comprise (i) the desired trait from the non-recurrent
parent and (ii) all of the physiological and morphological characteristics of spinach the recurrent
parent as determined at the 5% significance level when grown in the same environmental
conditions.
New varieties can also be developed from more than two parents. The technique,
known as modified backcrossing, uses different recurrent parents during the backcrossing.
Modified backcrossing may be used to replace the original recurrent parent with a variety having
certain more desirable characteristics or multiple parents may be used to obtain different
desirable characteristics from each.
With the development of molecular markers associated with particular traits, it is
possible to add additional traits into an established germ line, such as represented here, with the
end result being substantially the same base germplasm with the addition of a new trait or traits.
Molecular breeding, as described in Moose and Mumm, 2008 (Plant Physiology, 147: 969-977),
for example, and elsewhere, provides a mechanism for integrating single or multiple traits or
QTL into an elite line. This molecular breeding-facilitated movement of a trait or traits into an
elite line may encompass incorporation of a particular genomic fragment associated with a
particular trait of interest into the elite line by the mechanism of identification of the integrated
genomic fragment with the use of flanking or associated marker assays. In the embodiment
represented here, one, two, three or four genomic loci, for example, may be integrated into an
elite line via this methodology. When this elite line containing the additional loci is further
crossed with another parental elite line to produce hybrid offspring, it is possible to then
incorporate at least eight separate additional loci into the hybrid. These additional loci may
confer, for example, such traits as a disease resistance or a fruit quality trait. In one embodiment,
each locus may confer a separate trait. In another embodiment, loci may need to be homozygous
and exist in each parent line to confer a trait in the hybrid. In yet another embodiment, multiple
loci may be combined to confer a single robust phenotype of a desired trait.
Many single locus traits have been identified that are not regularly selected for in
the development of a new inbred but that can be improved by backcrossing techniques. Single
locus traits may or may not be transgenic; examples of these traits include, but are not limited to,
herbicide resistance, resistance to bacterial, fungal, or viral disease, insect resistance, modified
fatty acid or carbohydrate metabolism, and altered nutritional quality. These comprise genes
generally inherited through the nucleus.
Direct selection may be applied where the single locus acts as a dominant trait.
For this selection process, the progeny of the initial cross are assayed for viral resistance and/or
the presence of the corresponding gene prior to the backcrossing. Selection eliminates any plants
that do not have the desired gene and resistance trait, and only those plants that have the trait are
used in the subsequent backcross. This process is then repeated for all additional backcross
generations.
Selection of spinach plants for breeding is not necessarily dependent on the
phenotype of a plant and instead can be based on genetic investigations. For example, one can
utilize a suitable genetic marker which is closely genetically linked to a trait of interest. One of
these markers can be used to identify the presence or absence of a trait in the offspring of a
particular cross, and can be used in selection of progeny for continued breeding. This technique
is commonly referred to as marker assisted selection. Any other type of genetic marker or other
assay which is able to identify the relative presence or absence of a trait of interest in a plant can
also be useful for breeding purposes. Procedures for marker assisted selection are well known in
the art. Such methods will be of particular utility in the case of recessive traits and variable
phenotypes, or where conventional assays may be more expensive, time consuming or otherwise
disadvantageous. Types of genetic markers which could be used in accordance with the
invention include, but are not necessarily limited to, Simple Sequence Length Polymorphisms
(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990), Randomly Amplified
Polymorphic DNAs (RAPDs), DNA Amplification Fingerprinting (DAF), Sequence
Characterized Amplified Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-
PCR), Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858, specifically
incorporated herein by reference in its entirety), and Single Nucleotide Polymorphisms (SNPs)
(Wang et al., Science, 280:1077-1082, 1998).
F. Plants Derived by Genetic Engineering
Many useful traits that can be introduced by backcrossing, as well as directly into
a plant, are those which are introduced by genetic transformation techniques. Genetic
transformation may therefore be used to insert a selected transgene into a plant of the invention
or may, alternatively, be used for the preparation of transgenes which can be introduced by
backcrossing. Methods for the transformation of plants that are well known to those of skill in
the art and applicable to many crop species include, but are not limited to, electroporation,
microprojectile bombardment, Agrobacterium-mediated transformation and direct DNA uptake
by protoplasts.
To effect transformation by electroporation, one may employ either friable
tissues, such as a suspension culture of cells or embryogenic callus or alternatively one may
transform immature embryos or other organized tissue directly. In this technique, one would
partially degrade the cell walls of the chosen cells by exposing them to pectin-degrading
enzymes (pectolyases) or mechanically wound tissues in a controlled manner.
An efficient method for delivering transforming DNA segments to plant cells is
microprojectile bombardment. In this method, particles are coated with nucleic acids and
delivered into cells by a propelling force. Exemplary particles include those comprised of
tungsten, platinum, and preferably, gold. For the bombardment, cells in suspension are
concentrated on filters or solid culture medium. Alternatively, immature embryos or other target
cells may be arranged on solid culture medium. The cells to be bombarded are positioned at an
appropriate distance below the macroprojectile stopping plate.
An illustrative embodiment of a method for delivering DNA into plant cells by
acceleration is the Biolistics Particle Delivery System, which can be used to propel particles
coated with DNA or cells through a screen, such as a stainless steel or Nytex screen, onto a
surface covered with target cells. The screen disperses the particles so that they are not delivered
to the recipient cells in large aggregates. Microprojectile bombardment techniques are widely
applicable, and may be used to transform virtually any plant species.
Agrobacterium-mediated transfer is another widely applicable system for
introducing gene loci into plant cells. An advantage of the technique is that DNA can be
introduced into whole plant tissues, thereby bypassing the need for regeneration of an intact plant
from a protoplast. Modern Agrobacterium transformation vectors are capable of replication in E.
coli as well as Agrobacterium, allowing for convenient manipulations (Klee et al., Bio-
Technology, 3(7):637-642, 1985). Moreover, recent technological advances in vectors for
Agrobacterium-mediated gene transfer have improved the arrangement of genes and restriction
sites in the vectors to facilitate the construction of vectors capable of expressing various
polypeptide coding genes. The vectors described have convenient multi-linker regions flanked
by a promoter and a polyadenylation site for direct expression of inserted polypeptide coding
genes. Additionally, Agrobacterium containing both armed and disarmed Ti genes can be used
for transformation.
In those plant strains where Agrobacterium-mediated transformation is efficient, it
is the method of choice because of the facile and defined nature of the gene locus transfer. The
use of Agrobacterium-mediated plant integrating vectors to introduce DNA into plant cells is
well known in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S. Patent No.
,563,055).
Transformation of plant protoplasts also can be achieved using methods based on
calcium phosphate precipitation, polyethylene glycol treatment, electroporation, and
combinations of these treatments (see, e.g., Potrykus et al., Mol. Gen. Genet., 199:183-188,
1985; Omirulleh et al., Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature, 312:791-
793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986; Marcotte et al., Nature, 335:454,
1988). Transformation of plants and expression of foreign genetic elements is exemplified in
Choi et al. (Plant Cell Rep., 13: 344–348, 1994), and Ellul et al. (Theor. Appl. Genet.,
107:462–469, 2003).
A number of promoters have utility for plant gene expression for any gene of
interest including but not limited to selectable markers, scoreable markers, genes for pest
tolerance, disease resistance, nutritional enhancements and any other gene of agronomic interest.
Examples of constitutive promoters useful for plant gene expression include, but are not limited
to, the cauliflower mosaic virus (CaMV) P-35S promoter, which confers constitutive, high-level
expression in most plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985), including in
monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591, 1990; Terada and Shimamoto, Mol. Gen.
Genet., 220:389, 1990); a tandemly duplicated version of the CaMV 35S promoter, the enhanced
35S promoter (P-e35S);l the nopaline synthase promoter (An et al., Plant Physiol., 88:547,
1988); the octopine synthase promoter (Fromm et al., Plant Cell, 1:977, 1989); and the figwort
mosaic virus (P-FMV) promoter as described in U.S. Pat. No. 5,378,619 and an enhanced
version of the FMV promoter (P-eFMV) where the promoter sequence of P-FMV is duplicated in
tandem; the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform virus promoter; a
commelina yellow mottle virus promoter; and other plant DNA virus promoters known to
express in plant cells.
A variety of plant gene promoters that are regulated in response to environmental,
hormonal, chemical, and/or developmental signals can also be used for expression of an operably
linked gene in plant cells, including promoters regulated by (1) heat (Callis et al., Plant Physiol.,
88:965, 1988), (2) light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., Plant Cell, 1:471, 1989;
maize rbcS promoter, Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-binding
protein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones, such as abscisic acid
(Marcotte et al., Plant Cell, 1:969, 1989), (4) wounding (e.g., wunl, Siebertz et al., Plant Cell,
1:961, 1989); or (5) chemicals such as methyl jasmonate, salicylic acid, or Safener. It may also
be advantageous to employ organ-specific promoters (e.g., Roshal et al., EMBO J., 6:1155,
1987; Schernthaner et al., EMBO J., 7:1249, 1988; Bustos et al., Plant Cell, 1:839, 1989).
Exemplary nucleic acids which may be introduced to plants of this invention
include, for example, DNA sequences or genes from another species, or even genes or sequences
which originate with or are present in the same species, but are incorporated into recipient cells
by genetic engineering methods rather than classical reproduction or breeding techniques.
However, the term “exogenous” is also intended to refer to genes that are not normally present in
the cell being transformed, or perhaps simply not present in the form, structure, etc., as found in
the transforming DNA segment or gene, or genes which are normally present and that one
desires to express in a manner that differs from the natural expression pattern, e.g., to over-
express. Thus, the term "exogenous" gene or DNA is intended to refer to any gene or DNA
segment that is introduced into a recipient cell, regardless of whether a similar gene may already
be present in such a cell. The type of DNA included in the exogenous DNA can include DNA
which is already present in the plant cell, DNA from another plant, DNA from a different
organism, or a DNA generated externally, such as a DNA sequence containing an antisense
message of a gene, or a DNA sequence encoding a synthetic or modified version of a gene.
Many hundreds if not thousands of different genes are known and could
potentially be introduced into a spinach plant according to the invention. Non-limiting examples
of particular genes and corresponding phenotypes one may choose to introduce into a spinach
plant include one or more genes for insect tolerance, such as a Bacillus thuringiensis (B.t.) gene,
pest tolerance such as genes for fungal disease control, herbicide tolerance such as genes
conferring glyphosate tolerance, and genes for quality improvements such as yield, nutritional
enhancements, environmental or stress tolerances, or any desirable changes in plant physiology,
growth, development, morphology or plant product(s). For example, structural genes would
include any gene that confers insect tolerance including but not limited to a Bacillus insect
control protein gene as described in WO 99/31248, herein incorporated by reference in its
entirety, U.S. Pat. No. 5,689,052, herein incorporated by reference in its entirety, U.S. Pat.
Nos. 5,500,365 and 5,880,275, herein incorporated by reference in their entirety. In another
embodiment, the structural gene can confer tolerance to the herbicide glyphosate as conferred by
genes including, but not limited to Agrobacterium strain CP4 glyphosate resistant EPSPS gene
(aroA:CP4) as described in U.S. Pat. No. 5,633,435, herein incorporated by reference in its
entirety, or glyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No. 5,463,175,
herein incorporated by reference in its entirety.
Alternatively, the DNA coding sequences can affect these phenotypes by
encoding a non-translatable RNA molecule that causes the targeted inhibition of expression of an
endogenous gene, for example via antisense- or cosuppression-mediated mechanisms (see, for
example, Bird et al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be a
catalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desired endogenous mRNA
product (see for example, Gibson and Shillito, Mol. Biotech., 7:125,1997). Thus, any gene
which produces a protein or mRNA which expresses a phenotype or morphology change of
interest is useful for the practice of the present invention.
G. Definitions
In the description and tables herein, a number of terms are used. In order to
provide a clear and consistent understanding of the specification and claims, the following
definitions are provided:
Allele: Any of one or more alternative forms of a gene locus, all of which alleles
relate to one trait or characteristic. In a diploid cell or organism, the two alleles of a given gene
occupy corresponding loci on a pair of homologous chromosomes.
Backcrossing: A process in which a breeder repeatedly crosses hybrid progeny,
for example a first generation hybrid (F ), back to one of the parents of the hybrid progeny.
Backcrossing can be used to introduce one or more single locus conversions from one genetic
background into another.
Crossing: The mating of two parent plants.
Cross-pollination: Fertilization by the union of two gametes from different
plants.
Diploid: A cell or organism having two sets of chromosomes.
Emasculate: The removal of plant male sex organs or the inactivation of the
organs with a cytoplasmic or nuclear genetic factor or a chemical agent conferring male sterility.
Enzymes: Molecules which can act as catalysts in biological reactions.
F Hybrid: The first generation progeny of the cross of two nonisogenic plants.
Genotype: The genetic constitution of a cell or organism.
Haploid: A cell or organism having one set of the two sets of chromosomes in a
diploid.
Linkage: A phenomenon wherein alleles on the same chromosome tend to
segregate together more often than expected by chance if their transmission was independent.
Marker: A readily detectable phenotype, preferably inherited in codominant
fashion (both alleles at a locus in a diploid heterozygote are readily detectable), with no
environmental variance component, i.e., heritability of 1.
Phenotype: The detectable characteristics of a cell or organism, which
characteristics are the manifestation of gene expression.
Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer to genetic
loci that control to some degree numerically representable traits that are usually continuously
distributed.
Resistance: As used herein, the terms “resistance” and “tolerance” are used
interchangeably to describe plants that show no symptoms to a specified biotic pest, pathogen,
abiotic influence or environmental condition. These terms are also used to describe plants
showing some symptoms but that are still able to produce marketable product with an acceptable
yield. Some plants that are referred to as resistant or tolerant are only so in the sense that they
may still produce a crop, even though the plants are stunted and the yield is reduced.
Regeneration: The development of a plant from tissue culture.
Royal Horticultural Society (RHS) color chart value: The RHS color chart is a
standardized reference which allows accurate identification of any color. A color’s designation
on the chart describes its hue, brightness and saturation. A color is precisely named by the RHS
color chart by identifying the group name, sheet number and letter, e.g., Yellow-Orange Group
19A or Red Group 41B.
Self-pollination: The transfer of pollen from the anther to the stigma of the same
plant.
Single Locus Converted (Conversion) Plant: Plants which are developed by a
plant breeding technique called backcrossing, wherein essentially all of the morphological and
physiological characteristics of a spinach variety are recovered in addition to the characteristics
of the single locus transferred into the variety via the backcrossing technique and/or by genetic
transformation.
Substantially Equivalent: A characteristic that, when compared, does not show
a statistically significant difference (e.g., p = 0.05) from the mean.
Tissue Culture: A composition comprising isolated cells of the same or a
different type or a collection of such cells organized into parts of a plant.
Transgene: A genetic locus comprising a sequence which has been introduced
into the genome of a spinach plant by transformation.
H. Deposit Information
A deposit of spinach hybrid RX 06682141 and inbred parent spinach line SSB 66-
1131 M, disclosed above and recited in the claims, has been made with the American Type
Culture Collection (ATCC), 10801 University Blvd., Manassas, VA 20110-2209. The dates of
deposit were February 8, 2012, and August 19, 2011, respectively. The accession numbers for
those deposited seeds of spinach hybrid RX 06682141 and inbred parent line SSB 66-1131 M are
ATCC Accession No. PTA-12505 and ATCC Accession No. PTA-12041, respectively. Upon
issuance of a patent, all restrictions upon the deposits will be removed, and the deposits are
intended to meet all of the requirements of 37 C.F.R. §1.801-1.809. The deposits will be
maintained in the depository for a period of 30 years, or 5 years after the last request, or for the
effective life of the patent, whichever is longer, and will be replaced if necessary during that
period.
Although the foregoing invention has been described in some detail by way of
illustration and example for purposes of clarity and understanding, it will be obvious that certain
changes and modifications may be practiced within the scope of the invention, as limited only by
the scope of the appended claims.
All references cited herein are hereby expressly incorporated herein by reference.
Claims (17)
1. A seed of spinach hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505.
2. A plant grown from the seed of claim 1.
3. A plant part of the plant of claim 2.
4. The plant part of claim 3, wherein said part is selected from the group consisting of a fruit, an ovule, pollen, a leaf, or a cell.
5. A tissue culture of regenerable cells of spinach hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505.
6. The tissue culture according to claim 5, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and stalks.
7. A spinach plant regenerated from the tissue culture of claim 5, wherein the regenerated plant expresses all of the physiological and morphological characteristics of spinach hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505.
8. A method of producing spinach seed, comprising crossing the plant of claim 2 with itself or a second spinach plant.
9. A method for producing a seed of a hybrid RX 06682141-derived spinach plant comprising the steps of: (a) crossing a spinach plant of hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505, with a second spinach plant; and (b) allowing seed of a RX 06682141-derived spinach plant to form.
10. The method of claim 9, further comprising the steps of: (c) selfing the plant grown from said RX 06682141-derived spinach seed or crossing it to a second spinach plant to yield additional RX 06682141-derived spinach seed; (d) growing said additional RX 06682141-derived spinach seed of step (c) to yield additional RX 06682141-derived spinach plants; and (e) repeating the steps of (c) and (d) to generate further RX 06682141-derived spinach plants.
11. A method of vegetatively propagating a plant of spinach hybrid RX 06682141 comprising the steps of: (a) collecting tissue capable of being propagated from a plant of spinach hybrid RX 06682141, a sample of seed of said hybrid having been deposited under ATCC Accession Number PTA-12505; (b) cultivating said tissue to obtain proliferated shoots; and (c) rooting said proliferated shoots to obtain rooted plantlets.
12. The method of claim 11, further comprising growing plants from said rooted plantlets.
13. A method of producing a plant of spinach hybrid variety RX 06682141 comprising an added desired trait, the method comprising introducing a transgene conferring the desired trait into spinach hybrid RX 06682141, whereby a plant of spinach hybrid RX 06682141 comprising an added desired trait is produced, a sample of seed of said variety having been deposited under ATCC Accession Number PTA-12505.
14. A plant of spinach hybrid variety RX 06682141 comprising a transgene conferring a desired trait, a sample of seed of said variety has been deposited under ATCC Accession Number PTA-12505.
15. A seed that produces the plant of claim 14.
16. A method of producing a spinach plant part comprising: (a) obtaining the plant of claim 1, wherein the plant has been cultivated to maturity; (b) collecting at least a first plant part from the plant.
17. The method of claim 16, wherein the plant part comprises a leaf.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
USUS61/596,434 | 2012-02-08 | ||
USUS13/419,251 | 2012-03-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
NZ606397B true NZ606397B (en) | 2013-12-03 |
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