NZ538325A - Process for the preparation of tetrasubstituted imidazole derivatives and novel crystalline structures thereof - Google Patents

Abstract

A crystalline form of a compound of formula (II).

Description

New Zealand Paient Spedficaiion for Paient Number 538325 53 83 2 5 NEW ZEALAND PATENTS ACT, 1953 No: Divided out of No. 528405 Date: Dated 22 February 2002 COMPLETE SPECIFICATION PROCESS FOR THE PREPARATION OF TETRASUBSTITUTED IMIDAZOLE DERIVATIVES AND NOVEL CRYSTALLINE STRUCTURES THEREOF We, ORTHO-MCNEIL PHARMACEUTICAL, INC., incorporated in the State of Delaware, United States of America, of U.S. Route #202, P.O. Box 300, Raritan, New Jersey 08869-0602, United States of America, do hereby declare the invention for which we pray that a patent may be granted to us, and the method by which it is to be performed, to be particularly described in and by the following statement: (followed by page la) INTELLECTUAL PROPERTY OFFICE 1 a FEB 2005 ft £ C E ! VED CROSS REFERENCE TO RELATED APPLICATION This application claims priority from United States provisional application Serial No. 60/278,607, filed March 26, 2001, the contents of which are hereby incorporated by reference.

FIELD OF THE INVENTION The present invention relates to a process for preparing tetrasubstituted imidazole derivatives of the general formula (I) wherein R1, R2, R3 and R4 are as defined in the specification below. la and novel crystalline structures of the compound of formula (II).

BACKGROUND OF THE INVENTION The invention relates to a process for preparing tetrasubstituted imidazole derivatives represented by the formula (I) (I) wherein R1, R2, R3 and R4 are as defined in the specification below.

The compounds of formula (I) inhibit the in vitro activity of p3 8 in the nanomolar range. In addition, the 15 compounds inhibit the in vitro secretion of tumor necrosis factor a (TNF-a) and IL-P in the nanomolar range. Animal models demonstrate the inhibition of LPS induces TNF-a, as well as the inhibition of rheumatoid arthritis. The compounds of formula (I) are useful in the treatment of a 20 variety of cytokine related disorders including rheumatoid arthritis, inflammatory bowel disease, septic shock, osteoporosis, osteoarthritis, neuropathic pain, HIV replication, HIV dementia, viral myocarditis, insulin-dependent diabetes, non-insulin dependent diabetes, 25 periodontal disease, restenosis, alopecia areta, T-cell depletion in HIV infection or AIDS, psoriasis, acute pancreatitis, allograft rejection, allergic inflammation in the lung, atherosclerosis, multiple sclerosis, 2 cachexia, Alzheimer's disease, stroke, Crohn's disease, ischemia, congestive heart failure, pulmonary fibrosis, hepatitis, glioblastoma, Guillain-Barre Syndrome and systemic lupus erythematosus. (US Patent No. 5,965,583 5 Issued Oct 12, 1999) The current invention relates to an efficient process for preparing compounds of formula (I). In a further aspect, the present invention relates to a process for preparing the compound of formula (II).

OH The compound of formula (II) is an orally active inhibitor of p38 kinase. p38 kinase inhibitors have utility 15 in suppressing the release of TNF-a from monocytes and would be expected to suppress signal transduction initiated by this proinflammatory mediator. Thus p38 kinase inhibitors have utility in the treatment of various inflammatory and autoimmune disorders such as rheumatoid arthritis, sepsis, 20 inflammatory bowel disease, acute respiratory distress syndrome, as well as cachexia and bone resorption (osteoporosis and osteoarthritis). 3 The present invention further relates to novel crystalline structures of the compound of formula (II), more specifically Form A and Form B.

A process for synthesizing pyridyl imidazole compounds is disclosed in US Patent No. 5,670,527, issued Sept. 23, 1997 (Adams, J. L., et. al., SmithKline Beecham Corp. Assignee) and in PCT application WO 96/21452, published July, 18, 1996 (Adams, J. L., et. al., SmithKline Beecham 10 Corporation).

U.S. Patent No 5,965,583 (Issued Oct. 12, 1999), which is incorporated herein by reference, disclose a process for preparing the compounds of formula (I). This process 15 requires chromatographic separation of intermediates, making it unsuitable for large scale production.

Thus there exists a need for a process which is compatible with large scale production needs and which 2 0 achieves acceptable levels of purity and yield.

BRIEF SUMMARY OF THE INVENTION The invention relates to a process for preparing a 25 compound of formula (I): (I) wherein 4 R1 is selected from the group consisting of phenyl, substituted phenyl, (where the substituents are selected from Cx-Csalkyl, halogen or trifluoromethyl) and heteroaryl, where the heteroaryl contains 5 to 6 ring 5 atoms; R2 is selected from the group consisting of phenyl, substituted phenyl, (where the substituents are selected from Ci-C5alkyl, halogen or trif luoromethyl) and heteroaryl, where the heteroaryl contains 5 to 6 ring 10 atoms and is optionally Ci-C4alkyl substituted; R3 is selected from the group consisting of hydrogen, arylCi-C5alkyl, substituted arylCi-C5 alkyl, (where the aryl substituents are independently selected from one or more of Cx-Csalkyl, Ci-Csalkoxy, halogen, amino, Ci-Csalkylamino 15 or di (Ci-C5alkyl) amino) , phthalimidoCi-C5alkyl, succinimidoCi-C5alkyl, Cx-CsalkylcarbonylCi-Csalkyl, aryloxycarbonylCi-C5alkyl, and heteroarylCi-C5akyl, where the heteroaryl contains 5 to 6 ring atoms; . 4-c=c— (ch2)p—x R is * , where p is an integer from 0 to 9; X is selected from the group consisting of hydrogen, hydroxy, vinyl, substituted vinyl, (where one or more substituents are selected from fluorine or chlorine), ethynyl, substituted ethynyl (where the substituent is 25 selected from fluorine or chlorine) , Ci-Csalkyl, substituted Ci-C5alkyl (where the alkyl substituents are selected from one or more of Ci-C5alkoxy, trihaloalkyl, phthalamido or amino), C3-C7cycloalkyl, Ci-Csalkoxy, substituted Ci-C5alkoxy (where the alkyl substituents are 30 selected from phthalimido or amino), phthalimidooxy, phenoxy, substituted phenoxy (where the phenyl substituents are selected from Ci-C5alkyl, fluorine, chlorine or Ci-C5alkoxy), phenyl, substituted phenyl (where the phenyl substituents are selected from Ci-C5alkyl, fluorine, chlorine or Ci-C5alkoxy) , arylCi-C5alkyl, substituted arylCi-C5alkyl (where the aryl substituents are selected from Ci-Csalkyl, fluorine, chlorine or Cx-C5alkoxy) , arylhydroxyCi-C5alkylamino, Ci-Csalkylamino, di (Ci-C5alkyl) amino, nitrile, oxime, benzyloxyimino, Ci-C5alkyloxyamino, phthalimido, succinimido, Ci-C5alkylcarbonyloxy, phenylcarbonyloxy, substituted phenylcarbonyloxy (where the phenyl substituents are selected from Ci-C5alkyl, fluorine, chlorine or Ci-C5alkoxy) , phenylCi-C5alkylcarbonyloxy, (where the phenyl substituents are selected from Ci-C5alkyl, fluorine, chlorine or Ci-C5alkoxy) , aminocarbonyloxy, C3.-C5alkylaminocarbonyloxy, di (Ci-C5alkyl) aminocarbonyloxy, Ci~ C5alkoxycarbonyloxy, substituted Ci-C5alkoxycarbonyloxy (where the alkyl substituents are selected from the group consisting of methyl, ethyl, isopropyl and hexyl), phenoxycarbonyloxy, substituted phenoxycarbonyloxy (where the phenyl substituents are selected from Ci-C5alkyl, fluorine, chlorine or Ci-C5alkoxy) , Ci-Csalkylthio, substituted Ci-C5alkylthio (where the alkyl substituents are selected from hydroxy and phthalimido), Ci~ C5alkylsulfonyl, phenylsulfonyl and substituted phenylsulfonyl (where the phenyl substituents are selected from fluorine, chlorine, Ci-C5alkoxy or trif luoromethyl) ; or pharmaceutical^ acceptable salts thereof; comprising h ol1 (III) (IV) reacting an aldehyde of formula (III) to produce the corresponding compound of formula (IV), where L1 and L2 are independently selected from the group consisting of Cx-5 C4alkyl and C1-C4aralkyl; or L1 together with L2 is selected from the group consisting of -CH2-CH2- (optionally substituted with one to four C1-C3 alkyl) , and -CH2-CH2-CH2-(optionally substituted with one to six C1-C3 alkyl); H TMS R / f O R1 (V) :N (VI) in a separate reaction vessel, reacting an aldehyde of formula (V), with an alkali metal salt of bis (trimethylsilyl)amide, to produce the corresponding trimethylsilyl substituted imine of formula (VI); ol1 R2^ ^OL2 (IV) ol1 Li R2^ ^OL2 (VII) + r / :n tms r1 (VI) l1q l2o- nh2 r2 r1 (VIII) 7 reacting the compound of formula (IV) with an alkyl lithium to produce the corresponding lithium intermediate of formula (VII); reacting the lithium intermediate of formula (VII) with the trimethylsilyl substituted imine of formula (VI) to produce the corresponding compound of formula (VIII); r3—nh2 + / N (IX) N in a separate vessel, reacting a substituted amine of formula (IX) with N,N'-carbonyldiimidazole, to yield the corresponding compound of formula (X); l10 l20- R hn- r1 hn—-rd (XI) reacting the compound of formula (VIII) with the 15 compound of formula (X), to produce the corresponding compound of formula (XI) ; 8 .0 L10 L20- HN- 7 R1 HN—R (XI) R R1 R-3 -N 'N H =0 (XII) cyclizing the compound of formula (XI), under acid conditions of pH less than about 7, to produce the corresponding compound of formula (XII) ; R' R1 R -N y=o N H (XII) (XIII) reacting the compound of formula (XII) with P0Br3( PBrs, or in a mixture of PBr3 and Br2, to yield the corresponding compound of formula (XIII); R ■ R1 R / ^-N y N (XIII) -Br H—R (XIV) R R' R -N )-R4 // ~N (I) displacing the bromine on the compound of formula (XIII) by reacting with a compound of formula (XIV), to produce the corresponding compound of formula (I).

In another aspect, the present invention relates to a process for preparing the compound of formula (II). 9 In a further aspect, the present invention is directed to intermediates of formula (XI) and formula (XII), and a process for preparing same. In still another aspect of the present invention is a process for preparing 5 the intermediate compound of formula (XIII).

In a further aspect, the present invention is directed to novel crystalline structures of the compound of formula (II), wherein the crystalline forms are herein 10 referred to as Form A and Form B, which may be characterized by their respective X-ray powder diffraction patterns.

In a particular aspect, the subject of this 15 specification, the present invention provides a crystalline form of the compound of formula (II) r ~ (") comprising the following x-ray powder diffraction peaks: ANGLE °20 d-Spacing (A) Relative Intensity (%) 7.206 12.257 100.0 8.961 9.861 14.2 .617 8.326 24.8 12.438 7.110 14.0 .500 .712 33.7 16.458 .382 13.3 17.360 .104 17.2 17.879 4.957 37.6 18.343 4.833 19.2 18.665 4.750 31.8 (followed by page 10a) 19.126 4.637 16.1 19.943 4.448 21.9 .491 4.331 .8 21.469 4.135 52.9 21.891 4.057 59.8 22.371 3.971 58.7 22.778 3.901 12.0 23.159 3.837 51.0 23.870 3.725 .8 24.526 3.627 .5 24.704 3.601 .9 .113 3.543 14.7 26.368 3.377 11.0 27.674 3.221 .5 28.088 3.174 18.3 28.896 3.087 21.3 29.291 3 .047 19.4 .201 2.9568 .6 .501 2.9284 13 .3 Other aspects of the invention are the subject of New Zealand Patent Specification No. 528405.

DETAILED DESCRIPTION OF THE INVENTION As used herein, the term "alkyl" whether used alone or as part of a substituent group, includes straight, branched and cyclic chain alkyl groups. For example, alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, t-butyl, pentyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like.

As used herein, unless otherwise noted, "alkoxy" shall denote a group of the formula -O-(alkyl), for example, methoxy, ethoxy, n-propoxy, sec-butoxy, t-butoxy, n-hexyloxy and the like.

As used herein, unless otherwise noted, "aryl" shall refer to unsubstituted mono and fused aromatic rings such as phenyl, naphthyl, and the like.

As used herein, unless otherwise noted, "heteroaryl" shall denote any five or six membered monocyclic aromatic 10a ring structure containing at least one heteroatom selected from sulfur, oxygen and nitrogen. In the case of five-membered rings, the heteroaryl will contain one sulfur, oxygen or nitrogen atoms and, in addition, may contain up to 5 three additional nitrogen atoms. In the case of six-membered rings, the heteroaryl may contain up to three nitrogen atoms. Examples of such heteroaryls include, but are not limited to, pyridin-2-yl, pyridin-3-yl, pyridin-4-yl, pyrimidi-3-yl, furan-2-yl, furan-3-yl, thiophen-2-yl, thiophen-3-yl, pyridazinyl, triazinyl, thiazolyl, oxazolyl, pyrazolyl, and the like.

As used herein, unless otherwise noted, "aralkyl" shall mean any Ci-C5 alkyl group substituted with an aryl group such as phenyl, naphthyl and the like. For example, benzyl, phenylethyl, and the like.

As used herein, unless otherwise noted, "halogen" shall mean chlorine, bromine, fluorine and iodine.

As used herein, the term "alkali metal" shall refer to a Group I metal cation such as lithium, sodium, potassium and cesium cations.

With reference to substituents, the term "independently" means that when more than one of such substituents is possible, such substituents may be the same or different from each other.

During any of the processes of the present invention, it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned.

This may be achieved by means of conventional protecting 11 groups, such as those described in Protective Groups in Organic Chemistry, ed. J.F.W. McOmie, Plenum Press, 1973; and T.W. Greene & P.G.M. Wuts, Protective Groups in Organic Synthesis. John Wiley & Sons, 1991. The protecting groups may be removed at a convenient subsequent stage using methods known from the art.

The invention relates to a process for preparing compounds of formula (I) as more fully described in Scheme 10 1. 12 H OL1 R2"^0 r2/ ^ol2 (III) H R^O (V) R3—NH2 + (IV) OL1 J< Li R2^ ^OL2 " (VII) L10 KIU \ /NH2 l2o-V-( o2 r.1 TMS :N R R' (VIII) R1 (VI) (IX) N O O R\ ~ H N X (X) ^N' R O .A N NH L1Q l2o- R2 R1 \ Ml v<, R2 R1 (XII) T P ^ 3 HN— R3 (XI) R' R R 1 N )—Br H—R4 R 2 / N. 1/ n (XIII) (XIV) R // R4 (I) SCHEME 1 As set forth in Scheme 1 above, an aldehyde of formula (III) , a known compound or compound prepared by known methods, is reacted with an alcohol, diol or trialkoxymethane, preferably trimethoxymethane, preferably in the presence of methanol, in a solvent capable of azeotropic removal of water such as benzene, toluene, 13 xylene, and the like, in the presence of an acid such as sulfuric acid, p-toluenesulfonic acid, and the like, preferably sulfuric acid, at reflux temperature, to produce the corresponding compound of formula (IV).

(Sheldrake, P. W., Synth Commun. (1993) 23 (14), 1967-71) In a separate reaction vessel, an aldehyde of formula (V) , a known compound or compound prepared by known methods, is reacted with an alkali metal salt of 10 bis (trimethylsilyl)amide, preferably lithium bis (trimethylsilyl)amide, in an organic solvent such as tetrahydrofuran (THF), diethyl ether, t-butylmethylether (MTBE) , and the like, preferably THF, at a temperature in the range of about -20°C to about room temperature, 15 preferably at a temperature of about 0°C, to produce the corresponding trimethylsilyl (TMS) substituted imine of formula (VI). (Ojima, I., et. al. , Tetrahedron (1996), 52, 209-224) 2 0 The compound of formula (IV) is reacted with an alkyl lithium such as methyl lithium, ethyl lithium, n-butyl lithium, and the like, preferably n-butyl lithium, at a temperature which prevents decomposition of the lithium intermediate of formula (VII), preferably at a temperature 25 of less than or equal to about -20°C, in an organic solvent such as tetrahydrofuran (THF), diethylether, t-butylmethylether (MTBE), and the like, preferably THF, to produce the corresponding lithium intermediate of formula (VII).

The lithium intermediate of formula (VII) is reacted with the TMS substituted imine of formula (VI) in the 14 presence of an organic solvent such as tetrahydrofuran (THF), diethylether, t-butylmethylether (MTBE), and the like, preferably THF, preferably allowing warming of the reaction mixture to about room temperature, to produce the corresponding compound of formula (VIII).

In a separate reaction vessel, a substituted amine of formula (IX), a known compound or compound prepared by known methods, is reacted with W,N'-carbonyldiimidazole, a known compound, in an inert organic solvent, such as tetrahydrofuran (THF), diethylether, t-butylmethylether (MTBE), toluene, dichloromethane (DCM), and the like, preferably THF, preferably at room temperature, to produce the corresponding compound of formula (X).

The compound of formula (VIII) is reacted with the compound of formula (X), in an organic solvent such as toluene, tetrahydrofuran (THF), dimethylformamide (DMF), and the like, preferably toluene, at a temperature in the range of about 50-150°C, preferably for toluene, at about reflux temperature, to produce the corresponding compound of formula (XI) .

The compound of formula (XI) is cyclized in an acid such as formic acid, aqueous hydrochloric acid, and the like, preferably aqueous hydrochloric acid, preferably at a temperature in the range of about 80-150°C, most preferably at a temperature in the range of about 95-100°C, to yield the corresponding compound of formula (XII).

The compound of formula (XII) is reacted with phosphorus oxybromide (POBr3) or phosphorous pentabromide (PBr5) , preferably POBr3, in an amount equal to at least about 5 equivalents, in an inert organic solvent whose boiling point is greater than or equal to about 110°C, such as tetramethylenesulfone, xylene, toluene, and the like, preferably tetramethylenesulfone, preferably in an amount equal to about 2 weight equivalents, at a temperature of greater than or equal to about 110°C, preferably at a temperature of about 130°C, to yield the corresponding compound of formula (XIII).

Alternatively, the compound of formula (XII) is reacted with a mixture of PBr3 and Br2 (which produces PBr5 in situ) , wherein the ratio of PBr3 to Br2 is in the range of about 1:2 to about 2:1, preferably the ratio of PBr3 to 15 Br2 is about 1:1; wherein the amount of the PBr5 produced by the mixture of PBr3 and Br2 is in the range of about 3-3.5 equivalents; in a solvent such as P0C13 or in an inert organic solvent whose boiling point is greater than or equal to about 110°C, such as tetramethylenesulfone 20 (sulfolane), xylene, toluene, and the like, preferably P0C13; at a temperature in the range of about 10-45°C, preferably at a temperature in the range of about 20-35°C; to yield the corresponding compound of formula (XIII). 2 5 The bromine on the compound of formula (XIII) is displaced by reacting with a compound of formula (XIV), a known compound or compound prepared by known methods, in the presence of a Pd(II) catalyst such as diacetoxybis(triphenylphosphine) palladium 30 (Pd (OAc) 2 (Ph3P) 2,) , dichloro bis (triphenylphosphine) palladium (PdCl2 (Ph3P) 2) , and the like, or in the presence of a catalyst such as palladium acetate (Pd(0Ac)2) or 16 palladium chloride (PdCl2) , wherein the palladium acetate or palladium chloride catalyst is further in the presence of triphenylphosphine, preferably the catalyst is diacetoxybis(triphenylphosphine) palladium, preferably in the presence of a co-catalyst such as copper(I) iodide (Cul), Fe powder, and the like, preferably Cul, in the presence of an organic amine such as diisopropylamine, diisopropylethylamine (DIPEA), triethylamine (TEA), piperidine, and the like, or an inorganic base such as K2C03, Cs2C03, and the like, preferably an organic amine, more preferably diisopropylamine, optionally in an inert organic solvent such as THF, fc-butyl methyl ether (MTBE), diethyl ether, DMF, acetonitrile, and the like, with heating to a temperature in the range of about 60-100°C, preferably to a temperature of about 75°C, to produce the corresponding compound of formula (I).

Alternatively, the compound of formula (XI) may be prepared according to the process outlined in Scheme 2 wherein L1, L2, R1, R2 and R3 are as set forth above.

More particularly, the compound of formula (VIII) is reacted with a compound of formula (XV), in an inert organic solvent such as tetrahydrofuran (THF), dimethylformamide (DMF), toluene, and the like, preferably (VIII) (XI) SCHEME 2 17 THF, preferably at room temperature, to produce the corresponding compound of formula (XI).

In a preferred embodiment of the invention, the 5 process is used to prepare the compound of formula (II). Preferably, the compound of formula (II) is further purified by known methods such as recrystallization from an organic solvent such as toluene, methanol, acetone, acetonitrile, and the like or from a mixture of organic 10 solvents such as ethyl acetate/hexane, THF/toluene, ethyl acetate/toluene, and the like.

The present invention is further directed to novel crystalline structures of the compound of formula (II).

The crystalline forms of the compound of formula (II) may be prepared by recrystallization of the compound of formula (II) from a suitable organic solvent such as acetone, acetonitrile, THF/toluene mixture, and the like.

Recrystallization of the compound of formula (II) as described above will yield one of two novel crystalline forms, herein referred to as Form A and Form B. Form B is obtained by recrystallization from acetone or a mixture of THF:toluene, more preferably a 1:2 mixture of THF:toluene.

Form A is obtained by recrystallization from acetonitrile.

The novel crystalline forms of the compound of formula (II) may be characterized by their respective x-30 ray powder diffraction patterns utilizing a Siemens D5000T-T based powder diffractometer using CuKa radiation and the following system conditions: 18 a) CuKa, radiation, 35tnA, 40KV b) Optics • 1mm slit, Gobel mirrors, 0.6mm slit, & vertical soller slits between tube and sample • LiF monochromator between sample and detector c) Scan 5 to 35°20 at 0.02 Step Size at a rate of 1°20/minute d) TTK-450 variable temperature / humidity stage and holder Form A of the compound of formula (II) may be characterized by its X-ray diffraction pattern, which comprises the major peaks as listed in Table 1.

TABLE 1. FORM A POWDER X-RAY DIFFRACTION PEAKS ANGLE 20 d-Spacing (A) Relative Intensity (%) 6.599 13.384 .2 7. 817 11.300 14.4 11.676 7.573 33 .2 17.536 .053 11.6 18.428 4.811 38 .3 19.318 4.591 19.9 19.948 4.447 100.0 .852 4.256 .1 21.463 4.137 43 .3 23.260 3.821 39.5 23.883 3.723 80.6 24.804 3 .587 57.9 .119 3 .542 31.9 .579 3.480 12.3 19 26.251 3.392 21.5 26.725 3 .333 58 . 6 28.229 3 .159 11.4 .487 2 .9296 23 . 0 31.614 2 .8278 17.6 Form B of the compound of formula (II) may be characterized by its X-ray diffraction pattern, which comprises the major peaks as listed in Table 2.

TABLE 2. FORM B POWDER X-RAY DIFFRACTION PEAKS ANGLE °20 d-Spacing (A) Relative Intensity (%) 7.206 12.257 100 . 0 8.961 9.861 14.2 .617 8 .326 24.8 12.438 7.110 14 . 0 .500 .712 33.7 16.458 .382 13.3 17.360 .104 17 .2 17.879 4 . 957 37. 6 18.343 4 . 833 19.2 18.665 4 .750 31.8 19.126 4 . 637 16 .1 19.943 4 .448 21. 9 .491 4 .331 .8 21.469 4.135 52 . 9 21.891 4 . 057 59.8 22.371 3 . 971 58.7 22.778 3 . 901 12.0 23.159 3.837 51.0 23.870 3.725 . 8 24 .526 3 .627 .5 24 .704 3.601 .9 .113 3 .543 14 .7 26.368 3.377 11.0 27.674 3.221 .5 28.088 3.174 18 .3 28.896 3.087 21.3 29.291 3.047 19.4 .201 2.9568 .6 .501 2.9284 13 .3 The following examples describe the invention in greater detail and are intended to illustrate the invention, but not to limit it.

EXAMPLE 1 4- (Dimethoxvmethvl)pyridine To a solution of 4-pyridinecarboxaldehyde (100.00 g, 0.93 mol) and trimethylorthoformate (159.20 g, 1.50 mol) in methanol (180 mL) at 0°C, under N2, was added concentrated sulfuric acid (41 mL, 0.45 mol). The resulting white suspension was heated to reflux and stirred for 24 hours. The reaction solution became clear after 2h. After cooling to room temperature, the reaction mixture was poured slowly into a solution of 25 wt.% sodium methoxide (360 mL) in methanol (300 mL). The mixture was then concentrated in vacuo to a light brown thick oil. To this crude oil was added t-butylmethyl ether (500 mL), followed by the slow addition of water (40 mL) (to convert the inorganics to filterable solids).

After filtration through a pad of Celite, the filtrate was concentrated to yield a light brown oil. The crude oil 21 was vacuum distilled to yield the desired product as a colorless oil Yield: 88.91 g (62.4%) BP 69-71°C at 1mm Hg EXAMPLE 2 2,2-Dimethoxv-2- (4-Pvridvl) -1- (4-fluorophenvl) ethanamine Step A: To a stirred solution of 1M lithium bis(trimethylsilyl)amide in THF (300 mL, 0.30 mol), under N2, was added 4-fluorobenzaldehyde (37.23 g, 0.30 mol), dropwise at 0°C. The resulting mixture was stirred at room temperature for 30 min to yield a solution.

Step B: In a second flask, 4-dimethoxymethylpyridine (38.29 g, 0.25 mol) was mixed with THF (200 mL) and cooled to -20°C. To the solution was slowly added dropwise 2.5M n-butyl lithium in hexane (120 mL, 0.30 mol), with the temperature of the reaction solution maintained between -15 and -2 0°C. The resulting dark brown reaction mixture was stirred at -20°C for 15 min. To the reaction mixture was slowly added the solution from step A above. The temperature of the reaction solution was maintained below -15°C. After addition, the dark brown reaction mixture was stirred and warmed up to room temperature. The reaction mixture was quenched with 2N aqueous HCl (500 mL) to a pH of about 2.0, and the resulting layers were separated. The organic layer was extracted once with IN aqueous HCl (100 mL) . The combined aqueous layers were washed with ethyl acetate (2x150 mL) and then basified with addition of 50% aq. NaOH solution, to a pH of about 10. The basified mixture was extracted with ethyl acetate 22 (400 mL, 2 x 100 mL). The combined ethyl acetate extracts were washed with water (200 mL), brine (200 mL), and dried with Na2S04. After concentration in vacuo, the crude product was obtained as a thick brown oil.

Yield: 54.70 g (79%) EXAMPLE 3 N- (3-phenylpropyl)-lH-imidazole-l-carboxamide To a suspension of 1,1' -carbonyldiimidazole (33.00 g, 0.2 03 mol) in THF (100 mL) at room temperature under N2, was added 3-phenylpropylamine (25.00 g, 0.185 mol) in THF (50 mL), dropwise. The reaction mixture became clear during the addition of the 3-phenylpropylamine. After completion of the addition, the clear solution was stirred for 30 min at room temperature and then quenched with water (150 mL) and ethyl acetate (200 mL). The layers were separated and the organic layer washed with water (150 mL) , brine (150 mL) , and dried with Na2S04. The solvents were removed in vacuo to yield a white wax-like solid.

Yield: 47.50 g EXAMPLE 4 N-(3-phenylpropyl)-N'-\(2.2-dimethoxy-2-(4-pvridvl)-1- (4-fluorophenvl)ethyl) 1 urea A solution of 2, 2-dimethoxy-2-(4-pyridyl)-1-(4-fluorophenyl)ethanamine (51.12 g, 0.185 mol) and N-(3-phenylpropyl)-lH-imidazole-l-carboxamide (42.42 g, 0.185 mol) in toluene (300 mL) under N2, was stirred and heated to reflux temperature for 3h. The solution was cooled to room temperature and the dark brown solution was diluted with ethyl acetate (200 mL). The mixture was washed with water (2 x 200 mL), brine (200 mL), and dried with Na2S04. 23 The solvents were removed in vacuo to yield a brown solid which was recrystallized from a solvent mixture of ethyl acetate/hexane (1:1) to yield an off-white solid.

Yield: 38.00 g (47%) EXAMPLE 5 1.3-dihvdro-l-(3-phenvlpropyl)-4-(4-fluorophenvl)-5-(4-pyridvl)-2H-imidazlin-2-one N- (3-phenylpropyl) -iST'- [ (2, 2-dimethoxy-2- (4-pyridyl) -10 1-(4-fluorophenyl)ethyl)]urea (38.0 g, 86.8 mmol) was dissolved in formic acid (100 mL) to form a brown solution. The solution was heated to 95-100°C and stirred under N2 for 24 h. The solution was then cooled to room temperature, the formic acid was removed under reduced 15 pressure by rotoevaporator and the residue diluted with ethyl acetate (300 mL). The solution was basified with 6N NaOH to a pH of about 10. An off-white solid formed slowly in the organic layer. The clear aqueous layer was separated and extracted with ethyl acetate (50 mL). The 20 combined organic layers were diluted with t- butylmethylether (350 mL) and stirred for 30 min. The solid product was collected by filtration, washed with t-butylmethyl ether (100 mL) and air-dried for lh. The solid product was dried in a vacuum oven at room 25 temperature for 24h to yield the product as an off-white solid.

Yield: 18.11 g (58%) MP: 198-199.5°C EXAMPLE 6 2-Bromo-l-(3-phenvlpropyl)-4-(4-fluorophenvl)-5-(4-pyridvl) - Iff- imidazole HBr Salt 24 1,3-dihydro-l-(3-phenylpropyl)-4-(4-fluorophenyl)-5-(4-pyridyl)-2H-imidazolin-2-one (5.0 g, 13.4 mmol) was suspended in sulfolane (20.0 g,) and treated with P0Br3 (19.5 g, 68 mmol). The mixture was heated to 130°C and 5 stirred under N2 for 3-3.5h. The reaction solution was cooled to room temperature, diluted with t-butylmethyl ether (100 mL) and cooled further to 0°C. The reaction mixture was quenched slowly with 10% NaOH solution (120 mL) to a pH of about 10. The layers were separated and 10 the aqueous layer was extracted with t-butylmethyl ether (30 x 2 mL). The combined organic layers were washed with water (50 x 2 mL), brine (50 mL), and dried with Na2S04. The solvent was removed in vacuo and the residue dissolved in a mixture of ethyl acetate (100 mL) and methanol (5 15 mL). The solution was treated with 2.88M HBr solution in ethyl acetate (9.3 mL, 26.8 mmol). The resulting yellow suspension was warmed on a steam-bath. Methanol (5 mL) was added to the suspension, resulting in the formation of a solution, and the solution was stirred overnight at room 20 temperature (ca. 18 h). Ethyl acetate (50 mL) was then added slowly and the suspension was stirred for another lh. The precipitate was collected by filtration and washed with ethyl acetate (50 mL). The solid was dried in a vacuum oven at room temperature for 2h, to yield the 2 5 product as a yellowish solid.

Yield: 5.01 g (62%), MP: 214-216°C, (color change at 205°C) EXAMPLE 7 3 0 4-(4-Fluorophenvl)-2- (4-hydroxy-1-butvnyl)-1-(3- Phenvlnropvl)-5-(4-Pvridvl)imidazole To a stirred solution of 4-(4-fluorophenyl)-2-iodo-l-(3-phenylpropyl)-5-(4-pyridyl)imidazole (1.42 g, 2.74 mmol) and 3-butyn-l-ol (0.289 g,4.1 mmol) in diisopropylamine (10 mL) was added bis(acetato) -bis (triphenylphosphine) palladium (0.102 g, 0.14 mmol), followed by the addition of copper(I) iodide (0.052 g, 5 0.274 mmol). The mixture was stirred at 75°C for 4h. The reaction mixture was then cooled to room temperature and quenched with water (100 mL) . The mixture was extracted with ethyl acetate (2 x 50 mL) . The combined ethyl acetate extract was washed with water (2x30 mL), brine 10 (30 mL) , and dried with Na2S04. After removal of solvents, | the crude product was obtained as a brown solid.

The crude product was purified by recrystallization from a mixture of ethyl acetate/hexane to yield the product as a yellow solid.

Yield: 0.88 g (75%) MP: 121-122°C EXAMPT.Fi 8 1,3-Dihydro-l-(3-phenvlpropyl)-4-(4-fluorophenyl)-5-(4- 2 0 pvridvl)-2H-imidazol-2-one N- (3-phenylpropyl)-N' -[(2,2-dimethoxy-2-(4-pyridyl)-1-(4-f luorophenyl) ethyl) ] urea (224 g, 0.45 mol) was mixed with 4N HCl (800 g) and heated under reflux for 4-5 h (95-100°C) . Upon completion, the reaction was cooled to room 25 temperature and adjusted to pH 13 with 8N NaOH solution (480 g), resulting in precipitation of a solid product. The pH of the suspension was controlled to pH > 13 for 30 min, with addition of sodium hydroxide as needed. The suspension was centrifuged and the aqueous phase removed 3 0 and discarded. The solid was resuspended in 2N NaOH solution (1000 g) , centrifuged a second time and then re-suspended in water (2 X 1000 g, water phase pH 7). The 26 solid product was dried at 45-50°C, under vacuum (for about 4-5 days) , to a final water content of <2%, to yield the product as a tan solid.

Yield: 175 g EXAMPLE 9 4- (4-Fluorophenyl)-2-bromo-l-(3-phenvlpropvl)-5-(4- pyridvl)-imidazole 1,3-Dihydro-l-(3-phenylpropyl)-4-(4-fluorophenyl)-5-10 (4-pyridyl)-2H-imidazol-2-one (100 g, 0.26 mol) was mixed ) with P0Br3 (268.7 g, 0.93 mol) and sulfolane (200 g) and the reaction mixture was heated to a temperature of 120-125°C for 1-2 h. Upon completion, the reaction mixture was cooled to 40°C. Cautiously, over about 30 min, 2N NaOH 15 solution (53 g) was added. Additional 2N NaOH solution (53 g) was then added at faster rate. The reaction mixture was then cooled to 15-20°C and 4N NaOH solution (802 g) was added to adjust the solution to pH 7-8. The aqueous phase was extracted with t-butylmethyl ether (3 X 20 143 g) and the organic phases combined. To the combined organic phase was added t-butylmethyl ether (107 g). The solution was washed with water (2 X 150g), resulting in the precipitation of a solid, which was collected by filtration.

HBr Salt: A solvent exchange solution of t-butylmethyl ether to ethyl acetate was used for the crystallization of the HBr salt.

The t-butylmethyl ether phase was concentrated to 150g (about % volume), diluted with ethyl acetate (4 60 g) 27 and concentrated again to 160g. The resulting oil was dissolved in ethyl acetate (460 g), HBr gas (21g, 0.26mol) was introduced and the solution heated to reflux, resulting in a separate yellow oil layer. Methanol (80 g) was added to the boiling mixture (65°C) , resulting in formation of a solid. The solution was stirred and cooled to 20-25°C over about 4h. The mixture was stirred overnight and cooled to 5°C. Ethyl acetate (160g) was then added to the solution. The resulting precipitate was suction filtered and washed with ethyl acetate (10 g) to yield the crude product as a yellow solid.

Isolation and Crystallization of the Free Base: The crude product (63 g) was dissolved in ethyl acetate (567 g) and mixed with saturated NaHC03 solution (126 g) . The mixture was stirred at 18-25°C for about 2h, until no further gas evolution was ascertainable. The aqueous phase was maintained at pH 8-9 with the addition of more saturated NaHC03 solution, as needed. The phases were separated and the organic phase was concentrated to about 1/3 volume. The resulting oil was dissolved in ethyl acetate (lOOg) and concentrated to dryness. The oil was suspended in acetone (95 g) and heated at reflux (56°C ± 2°C) for lh. The mixture was cooled over 3h to 36-30°C, held at this temperature for 2h, cooled to -10°C and held at this temperature for 2h. The resulting solid was vacuum filtered and washed with t-butylmethyl ether (10 g). The mother liquor was concentrated, mixed with acetone (41 g) , heated to reflux and cooled according to the above procedure to yield a second crop of product. The solid products from both crops were dried for l-2h at 40 deg/50 mbar to yield the product as a tan solid. 28 Yield: 34 g (30-32%) EXAMPLE 10 4-(4-Fluorophenvl)-2-(4-hydroxy-1-butinvl)-1-(3-5 phenylnropvl)-5-(4-Pvridvl)-imidazole 4-(4-Fluorophenyl)-2-bromo-l-(3-phenylpropyl)-5-(4-pyridyl)-imidazole (30.19 g) was mixed with diisopropylamine (100.56 g). To the reaction mixture was added 3-butyn-l-ol (5.304 g) , dropwise using a syringe. 10 Diisopropylamine (1.810 g) was then added to the reaction ^ mixture to wash the syringe, followed by addition of triphenylphosphine (1.805 g), Pd(0Ac)2 (0.722 g) , iron powder (0.384 g) , and diisopropylamine (78.64 g). The flask was briefly blanketed with nitrogen, then warmed to 15 70°C, and maintained at this temperature for 3h.

The above experiment was repeated several times. If the conversion was determined to be less than 95% after three hours, additional triphenylphosphine (1.805 g) and 20 palladium acetate (Pd(OAc)2) (0.772 g) were added and the temperature maintained until conversion of >95% was achieved.

Upon completion, the reaction mixture was filtered to 25 collect the solid residue. The filtered residue was suspended with ethyl acetate (212.17 g). at 40-50°C, filtered and solvent evaporated to dryness. The resulting oil was dissolved completely in the first filtrate at 70°C.

Water (148.608 g) was added to the hot solution and the 3 0 phases were separated. The organic phase was washed twice with water (148.608 g) at 70°C. The phases were separated again and the organic phase washed with brine (148.608 g) 29 and extracted with IN HCl (2 X 146 g). The combined HCl phases were re-extracted with ethyl acetate (99.07 g). The water phase was separated. To the water phase was added 25% ammonia (26.948 g) dropwise, with cooling to 5-5 10°C and at a pH 9-10, resulting in formation of a solid. The suspension was stirred for about 45 min and the precipitate collected by filtration. The precipitate was slurried with water (2 X 148.61 g) and then dried for 16h at 40°C/50 mbar. The solid was dissolved in a mixture of 10 ethyl acetate (412.21 g) and methanol (35.270 g), and mixed with Deloxan® (5.00 g) . The solution was stirred for 24h at 18-23°C and filtered. The filtered residue was washed with ethyl acetate (2 X 15.34 g). The combined mother liquors and washes were rotoevaporated to dryness. 15 The residue was dissolved in a mixture of THF (7..94 g) and toluene (16.0 g) at 70-75°C, cooled slowly over about 2h to 18-23°C, resulting in formation of a suspension. Toluene (9.2 g) was then added to the suspension. The suspension solids were suction filtered, washed with 20 toluene (3 X 1.40 g), and then washed with hexane (3 X 2.02 g) . The residue was dried for 16h at 50°C/50 mbar to yield the product as an off white yellow solid.

Yield: 20.5 g (70.5%) The above experiment was repeated several times.

Recrystallization of the above residue as described yielded Form B of the product. Recrystallization of the above residue from acetonitrile, yielded Form A of the product.

EXAMPLE 11 N- (3-phenylpropyl)-N'-T(2.2-dimethoxy-2-(4-pyridvl) -1-(4-fluorophenyl) ethvl) 1 urea To a solution of 2,2-dimethoxy-2-(4-pyridyl)-1-(4-fluorophenyl)ethanamine (1.22 g, 4.4 mmol) in THF (10 mL) 5 was added a solution of (3-isocyanatopropyl)-benzene (1.61 g, 10 mmol) in THF (10 mL) . The resulting mixture was stirred at room temperature for 3 0 minutes. The reaction was quenched by addition of water (50 mL) and extracted with ethyl acetate (2 X 50 mL) . The combined ethyl 10 acetate extract was washed with water (50 mL), brine (50 mL) and dried with Na2S04. After removal of solvent, the crude product was purified by column chromatography to yield the product as a light brown solid.

Yield: 0.83 g (43%) m.p. 162.5-165.5°C EXAMPLE 12 Synthesis of 4-(4-Fluorophenyl)-2-bromo-l-(3- phenylpropyl)-5-(4-pyridyl)-imidazole A reaction vessel was charged with POCl3 (1500.Og, 9.78 mol). Br2 (184.9g, 1.157 mol) was then added in one portion at ambient temperature. The reaction mixture was cooled to 10°C and then PBr3 (313.Og, 1,157 mol) was added over 25 min. under vigorous stirring. The temperature of the reaction mixture increased to 20°C. After addition, stirring was continued for another 1.5h, maintaining the temperature in the range of 10-20°C. The formed PBr5 precipitated as a yellow solid. The reaction mixture was warmed to 25°C and 1,3-dihydro-1-(3-phenylpropyl)-4-(4- fluorophenyl)-5-(4-pyridyl)-imidazol-2-one (150.Og 0,386 mol) was added in one portion. After addition, the reaction mixture was heated to about 3 0°C and stirring was continued for 24h. The suspension changed to a dark 31 solution. The POCl3 was distilled off under vacuum at a temperature below 35°C, to yield a viscous oil. This oil was added to a mixture of ethyl acetate (lOOOg) and aqueous ammonia (25 w%, lOOOg) over about 1.25 hours, with 5 cooling. The resulting two phases were separated, the aqueous phase was extracted with ethyl acetate (500g) and the combined organic phases were washed with water (200g) at 70°C. The organic phase was concentrated to approximately 30% of the original volume. To the warmed 10 reaction mixture, was then added triethylamine (600g) and | an additional amount of the solvent (about 150g) was removed in vacuo, resulting in the crystallization of the desired product. The reaction mixture was cooled to 0°C and stirred for 12h. The product was filtered off, washed 15 with triethylamine (50g) and dried at 40°C under vacuum, to yield the crude product.

The mother liquor was concentrated to an oil. To the oil was then added acetone (25g), resulting in precipitation of the desired product. The precipitate was 20 filtered off, washed with acetone (7g), then with methyl tert-butylether (8g) and dried at 40°C under vacuum to yield a second crop of the crude product.

Both crops of the isolated product were slurried in a mixture of triethylamine (lOg) and acetone (lOOg), under 25 reflux for 30 min, then cooled to 25°C and stirred overnight. The precipitate was filtered off, washed with triethylamine (25g), then with acetone (lOg) and dried at 40°C under vacuum to yield the title compound.

HPLC purity: 99% While the foregoing specification teaches the principles of the present invention, with examples provided for the purpose of illustration, it will be understood that 32 the practice of the invention encompasses all of the usual variations, adaptations and/or modifications as come within the scope of the following claims and their equivalents. 33

Claims (2)

WHAT WE CLAIM IS:

1. A crystalline form of the compound of formula (II) ANGLE °20 d-Spacing (A) Relative Intensity (%) 7.206 12 .257 100.0 8.961 9.861 14.2 10.617 8.326 24.8 12.438 7.110 14. 0 15 .500 5.712 33 .7 16.458 5.382 13.3 17.360 5.104 17.2 17.879 4.957 37.6 18.343 4.833 19.2 18.665 4.750 31.8 19.126 4.637 16.1 19.943 4.448 21.9 20.491 4 .331 30.8 21.469 4.135 52.9 21.891 4.057 59.8 22.371 3.971 58.7 22.778 3.901 12.0 23.159 3 .837 51.0 23.870 3 .725 20.8 24.526 3.627 15.5 24.704 3.601 25.9 25.113 3.543 14 .7 26.368 3.377 11.0 27.674 3.221 10.5 28.088 3.174 18.3 28.896 3.087 21.3 29.291 3 .047 19.4 30.201 2.9568 10.6 30.501 2.9284 13 .3 34 53 83 2 5

2. The crystalline form of claim 1, substantially as herein described. INTELLECTUAL fpnprRTv Or ■■,!.} " " l,Lj 18 FE3 2C35 I I REC£n/;-n I CoTW<N\cU<;v\ PWm&ce^vt.^, \r>c. By the authorised agents A J PARK Per 35