NZ240079A - Method for the detection of a nucleic acid or part thereof - Google Patents

Method for the detection of a nucleic acid or part thereof

Info

Publication number
NZ240079A
NZ240079A NZ240079A NZ24007991A NZ240079A NZ 240079 A NZ240079 A NZ 240079A NZ 240079 A NZ240079 A NZ 240079A NZ 24007991 A NZ24007991 A NZ 24007991A NZ 240079 A NZ240079 A NZ 240079A
Authority
NZ
New Zealand
Prior art keywords
nucleic acid
sample
solid phase
reacted
detection
Prior art date
Application number
NZ240079A
Inventor
Christoph Kessler
Ruediger Rueger
Cornelia Kruse-Mueller
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4038804A external-priority patent/DE4038804A1/en
Priority claimed from DE4041608A external-priority patent/DE4041608A1/en
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of NZ240079A publication Critical patent/NZ240079A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Control Of El Displays (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A process is disclosed for specifically detecting nucleic acids in a sample. The sample is reacted with one or several labeled nucleotide triphosphates and one or several enzymes which catalyze the production of a labeled nucleic acid B containing said nucleotide. Then the sample is subjected to a non thermal denaturation and reacted with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group. The possibly formed nucleic acid hybrid D is contacted with a solid phase which recognizes and binds the immobilizable group, the liquid phase is removed from the solid phase and the labeling of the solid phase is considered as a measure of the presence of nucleic acids.
NZ240079A 1990-10-09 1991-10-03 Method for the detection of a nucleic acid or part thereof NZ240079A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4032024 1990-10-09
DE4038804A DE4038804A1 (en) 1990-10-09 1990-12-05 METHOD FOR GENUS AND / AND SPECIES-SPECIFIC DETECTION OF BACTERIA IN A SAMPLING LIQUID
DE4041608A DE4041608A1 (en) 1990-12-22 1990-12-22 Sensitive and selective nucleic acid detection

Publications (1)

Publication Number Publication Date
NZ240079A true NZ240079A (en) 1993-07-27

Family

ID=27201764

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ240079A NZ240079A (en) 1990-10-09 1991-10-03 Method for the detection of a nucleic acid or part thereof

Country Status (15)

Country Link
EP (1) EP0552203B1 (en)
JP (1) JP2673162B2 (en)
AT (1) ATE122104T1 (en)
AU (1) AU655781B2 (en)
CA (1) CA2093647C (en)
DE (1) DE59105402D1 (en)
DK (1) DK0552203T3 (en)
ES (1) ES2073771T3 (en)
FI (1) FI103808B (en)
IE (1) IE68525B1 (en)
IL (1) IL99663A (en)
NO (1) NO305995B1 (en)
NZ (1) NZ240079A (en)
PT (1) PT99177B (en)
WO (1) WO1992006216A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08511425A (en) * 1993-06-09 1996-12-03 ガメラ バイオサイエンス コーポレイション Magnetic cycle reaction
DE4344742A1 (en) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Method for the immobilization of nucleic acids
WO1997038131A1 (en) * 1996-04-11 1997-10-16 Valentina Filippovna Kulikova Method for detecting a nucleic acid chain to be analysed
DE60024464T2 (en) * 1999-05-07 2006-08-03 Roche Diagnostics Gmbh HIGH DENSITY MARKING OF DNA WITH MODIFIED OR CHROMOPHORE-CARRYING NUCLEOTIDES AND USED DNA POLYMERASES
KR101078977B1 (en) 2002-11-07 2011-11-01 세키스이 메디칼 가부시키가이샤 Method of detecting gene mutation

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
AT390080B (en) * 1988-07-29 1990-03-12 Lion Thomas Dr FAST BLOT METHOD FOR DETECTING VIRAL SEQUENCES AND AMPLIFIED GENE SEQUENCES IN HUMAN CELLS
CA2004326A1 (en) * 1988-12-23 1990-06-23 Nanibushan Dattagupta Assay of sequences using amplified genes
KR920700360A (en) * 1989-03-22 1992-02-19 하리크 프리드리히 Sliding bearing
CA2012982A1 (en) * 1989-03-27 1990-09-27 Frank W. Hobbs Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes

Also Published As

Publication number Publication date
ATE122104T1 (en) 1995-05-15
NO931309D0 (en) 1993-04-06
IE68525B1 (en) 1996-06-26
IE913552A1 (en) 1992-04-22
FI931586A (en) 1993-04-07
PT99177A (en) 1992-09-30
PT99177B (en) 1999-04-30
NO305995B1 (en) 1999-08-30
WO1992006216A1 (en) 1992-04-16
AU655781B2 (en) 1995-01-12
DE59105402D1 (en) 1995-06-08
FI103808B1 (en) 1999-09-30
CA2093647C (en) 2000-09-19
IL99663A (en) 1995-12-31
AU8627591A (en) 1992-04-28
EP0552203A1 (en) 1993-07-28
ES2073771T3 (en) 1995-08-16
FI931586A0 (en) 1993-04-07
IL99663A0 (en) 1992-08-18
CA2093647A1 (en) 1992-04-10
DK0552203T3 (en) 1995-10-02
FI103808B (en) 1999-09-30
NO931309L (en) 1993-04-06
JPH06501157A (en) 1994-02-10
EP0552203B1 (en) 1995-05-03
JP2673162B2 (en) 1997-11-05

Similar Documents

Publication Publication Date Title
CA1272443A (en) Solution-phase dual hybridization assay for detecting polynucleotide sequences
DE3785865D1 (en) QUICK METHOD FOR DETECTING NUCLEIC ACID SEQUENCES IN A SAMPLE BY MEANS OF TEST MARKING.
CA2321185A1 (en) Method for detection of target nucleic acids using pcr
SE8600011L (en) METHOD OF IDENTIFICATION OF NUCLEIC ACIDS
ES529179A0 (en) A METHOD OF DETECTING A SEQUENCE OF POLYNUCLEOTIDE.
AU548854B2 (en) A method and reagent combination for the diagnosis of microorganisms
ES2194022T3 (en) METHOD FOR DETERMINING THE PRESENCE OF AN ORGANISM IN A PHYSIOLOGICAL SAMPLE.
EP0153873A3 (en) Detecting polynucleotide sequences
ES2177590T3 (en) DETECTION OF AMPLIFICATION OF NUCLEIC ACIDS.
BR9004881A (en) PROCESS TO DETECT A NUCLEIC ACID SEQUENCE, PROCESS TO QUANTITATIVELY DETERMINE THE EXTENSION OF HYBRIDIZATION, HYBRIDIZATION TEST, CASE, BASES, PROBES, PROCESS TO DETECT CHLAMYDIA TRACHOMATIS AND LITTLE LAMPS.
NO852022L (en) NUCLEIC ACID HYBRIDIZATION ANALYSIS USING DETECTABLE ANTI-HYBRID ANTIBODIES.
EP0892070A3 (en) Integrated process and system for the amplification and detection of nucleic acids
DK0407789T3 (en) Method for detecting nucleic acids
CA2268753A1 (en) Nucleic acid primers and probes for detecting oncogenic human papillomaviruses
DK0408918T3 (en) Method for Detecting Nucleic Acids
WO1991017264A1 (en) Assay for specific nucleic acid sequences
AU1010688A (en) Diagnostic assays using nucleic acid probes
EP0237833A3 (en) Solution-phase hybridization assay for detecting polynucleotide sequences
WO1999024611A3 (en) Analyte detection process using dual labeled probes
NZ240079A (en) Method for the detection of a nucleic acid or part thereof
EP0843019A3 (en) Method for determination of specific nucleic acid sequence, and a reagent therefor
WO2001011078A8 (en) Amplification method for detection of target nucleic acids involving fluorescence energy transfer
EP0366813A4 (en) Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms
GR3005459T3 (en)
JPS6446650A (en) Nucleic acid hybridization assay method

Legal Events

Date Code Title Description
RENW Renewal (renewal fees accepted)