NZ199828A

NZ199828A - 4-substituted mercapto-2-oxo-1-azetidine-sulphonic acid derivatives and pharmaceutical compositions

Info

Publication number: NZ199828A
Application number: NZ199828A
Authority: NZ
Inventors: U D Treuner; T Denzel; H Breuer; C M Cimarusti
Original assignee: Squibb & Sons Inc
Priority date: 1981-03-30
Filing date: 1982-02-24
Publication date: 1985-11-08
Also published as: EP0061763B1; EP0061763A1; IE820750L; DE3271742D1; DK142582A; GR76465B; AU557649B2; IL65146A0; ES518920A0; AU8076682A; ES8305711A1; ES8405764A1; PT74671B; PT74671A; PH20040A; ES510903A0; PH21999A; IE52723B1

Description

<div class="application article clearfix" id="description"> New Zealand Paient Spedficaiion for Paient Number 1 99828 1998 28 Priority Dats(s): 3P. 7?. 7?.!/. }Mt7. ."P.! Complete Specification Filed: Class: 7 _D U-oj5~ - -7 j) tic> <=» . J,. CjC* <r> ^ , >t Publication Date: ..I P.O. Journal, No: ..... ,/.?,77 f| PI ® P| irlMuiyil No.: Date: NEW ZEALAND PATENTS ACT, 1953 COMPLETE SPECIFICATION "4-SUBSTITUTED MERCAPTO-2-OXO-1-AZETIDINESULFONIC ACID SALTS" + /We, E. R. SQUIBB & SONS, INC., a corporation of Delaware having its offices at Lawrencevilie-Princeton Road, Princeton, New Jersey 08540, United States of America, hereby declare the invention for which i / we pray that a patent may be granted to me/us, and the method by which it is to be performed, to be particularly described in and by the following statement:- - 1 - (followed by iaj GCl67a 199m -im- Antibacterial activity is exhibited by 6-lactams having the formula 10 15 20 R1 -NH I 1 C- O r9 I 2 JS -c-r-. S I 3 -ch r4 •n-so.®m®, In -formula I, and throughout the specification, the symbols are as defined below. R is hydrogen or methoxy; R1 is acyl; R2 and R^ are each independently hydrogen or alkyl, or R2 and R3 when taken together with the carbon atom to which they are attached form a cycloalkyl group; R^ is hydrogen, alkyl, aryl, 2,3, or 4-pyridyl, 2 or 3-furyl, 2 or 3-thienyl, 0 1/ -C-R 5' 0 II -c-nr,r_, or -c=n; 6 7 25 r<- is hydrogen, alkyl/ aryl, arylalkyl, hydroxy"!, -0°M®, wherein M® is a cation, alkoxy, aryloxy, or arylalkoxy; r, and r_ are the same or different and b 7 each is hydrogen, alkyl, aryl or arylalkyl; 0 M is hydrogen or a cation. -2- GCl67a 199M8 Listed below are definitions of various terms' used to describe the b-lactams of this invention. These definitions apply to the terms as they are used throughout the specification (unless they are otherwise limited in specific instances) either individually or as part of a larger group. The terms "alkyl" and "alkoxy" refer to. both straight and branched chain groups. Those groups having 1 to 10 carbon atoms are preferred. The terms "alkanoyl" and "alkenyl" refer to both straight and branched chain groups. Those groups having up to 10 carbon atoms are preferred. -3- GC167a l$$ps 10 15 20 25 30 The terras "cycloalkyl" and "cycloalkenyl" refer to groups having 3,4,5,6 or 7 carbon atoms. The term "halogen" refers to fluorine, chlorine, bromine and iodine. The term "aryl" refers to phenyl and phenyl substituted with 1,2 or 3 amino (-Nt^), halogen, hydroxyl, trifluoromethyl, alkyl (of 1 to 4 carbon atoms), or alkoxy (of 1 to 4 carbon atoms) groups. The term "acyl" includes all organic ' radicals derived from an organic acid (i.e., a carboxylic acid) by removal of the hydroxyl group. Certain acyl groups are, of course,' ~ -preferred but this preference should not be viewed as a limitation of the scope of this invention. Exemplary^acyl groups are those acyl groups which have been used in the past to acylate 0-lactam antibiotics including 6-aminopenicillanic acid and derivatives and 7-aminocephalosporanic acid and derivatives; see, for example, Cephalosporins and Penicillins, edited by Flynn, Academic Press (1972), .. United States patent 4,152,432, issued May 1, 1979, United States patent 3,971,778, issued July 27, 1976, United States"patent 4,172,199, issued October 23, 1979, and British patent 1,348,894, published March 27, 19 74. The-portions of these references describing various acyl groups are incorporated s- GC16 7a -4- (1998 2 8 herein by reference. The following list of acyl groups is presented to further exemplify the . term "acyl"; it should not be regarded as limiting that term. Exemplary acyl group's are: 5 (a) Aliphatic groups having the formula 0 II ' R8~C~ wherein R_ is alkyl; cycloalkyl; alkoxy; alkenyl; O 10 cycloalkenyl; cyclohexadienyl; or alkyl or alkenyl substituted with one or more halogen, cyano, nitro, amino, mercapto, alkylthio, or cyanomethyl-thio groups. (b) Carbocyclic aromatic groups having the 15 formula 0 II (CHO -C-, 2 n ?10 9^%Rl1 " "y—CH-C- , R12 ^10 II —Cll2-0-C-, CC16 7 a -5- *JlO R_ _Ll R„ 0 1998 9 ^-ch2-C-, 10 R9j<Okfll ° \_7-ch2-s-c- 15 wherein n is 0, 1, 2 or 3; Rg, R^q , and R^ each is independently hydrogen, halogen, hydroxyl, nitro, amino, cyano, trifluoromethyl,. alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 4 carbon atoms or aminomethyl; and R^ i-s amino, hydroxyl, 20 a carboxyl salt, protected carboxyl, formyloxy, a sulfo salt, a sulfoamino salt, azido, halogen, hydrazino, alkylhydrazino, phenylhydrazino, or [ (alkylthio)thioxomethyl]thio. Preferred carbocyclic aromatic acyl groups 25 include those having the formula 0 HO —1 )— CH2~C-, -6- GCl67a 1MS28 HO- R 0 II H-C-12 (R-^2 is preferably a carboxyl salt or sulfo salt) and 10 o ii CH-C-I R12 (R^2 is preferably 15 20 25 a carboxyl salt or sulfo salt). (c) • Heteroaromatic groups having the formula O .R^-tCH, )„-"-, 0 II R. -,-CH-C- , 13 | R 12 0 II R13~0-CH2~C~'' 0 II R13-S-CH2~C- , or R 13 0 O 4 c- 30 wherein n is 0, 1, 2 or 3; R^2 as defined above; and R13 is a substituted or unsubstituted 5-, 6- or 7-membered heterocyclic ring containing 1, 2, 3 or 4 (preferably 1 or 2) heteroatous selected from nitrogen, oxygen and sulfur atoms. Exemplary heterocyclic h 1-1 GC167 a -7- 11998 2 rings are thienyl, furyl, pyrrolyl, pyridinyl, pyrazolyl, pyrazinyl, thiazolyl, morpholinyl, pyrimidinyl and tetrazolyl. Exemplary substituents are halogen, hydroxyl, nitro,.amino, cyario, 5 trifluoromethyl, alkyl of. 1 to 4 carbon atoms, alkoxy . 0 II , of 1 to 4 carbon atoms, or H00C-CH-CH--0-C-NH. I ^ nh2 Preferred heteroaromatic acyl groups 10 include those groups of the above formulas wherein i-s 2-amino-4-thiazolyl, 2-amino-5- halo-4-thiazolyl, 4 -aminopyrim'idin-2-yl, 5-amino-l,2,4-thiadiazol-3-yl, 2-thienyl, 2-furanyl, or 6-aminopyridin-2-yl. 15 (d) [[(4-Substituted-2,3-dioxo-l-piper- azinyl)carbonyl]amino]arylacetyl groups having the formula 0 O / v II II / \ -C-CH-NH-C-N N-R, _ 20 • A... ■ ^ 14 o 0 wherein R^ is an aromatic group (including carbocyclic aromatics such as those of the formula 25 Rc and heteroaromatics as included within the definition of and R^<- is alkyl, substituted alkyl (wherein the alkyl group is substituted 30 with one or more halogen, cyano, nitro, amino GC16 7a -8- or mercapto groups), arylmethyleneamino (i.e., -N=CH-R^, wherein R^ is as defined above), 0 II arylcarbonylaramo (i.e. , -NH-C-R^ wherein R^4 5 is as defined above) or alkylcarbonylamino. Preferred [I (4-substituted-2,3-dioxo-l- 4 piperazinyl)carbonyl]amino]arylacetyl groups include those wherein R^ is ethyl, phenylmethylene- amirio or 2-furylmethyleneamino. 10 (e) (Substituted oxyiraino)arylacetyl groups having the formula O II -C-C=N-0-R,, t 16 Ri4: 15 wherein R ^ is as defined above and R^g is hydrogen, alkyl, cycloalkyl, alkylaminocarbonyl, arylamino- O carbonyl (i.e., -C-NH-R ^ wherein R^ is as defined ' 20 above)- or substituted alkyl (wherein the alkyl group is substituted with 1 or more halogen, cyano, nitro, amino, mercapto, alkylthio, aromatic group (as defined by R^^), carboxyl (including salts thereof), aminocarbony1, alkoxycarbonyl, 25 phenylmethoxycarbonyl, diphenylmethoxycarbonyl, hydroxyalkoxyphosphinyl, dihydroxyphosphinyl, hydroxy (phenylmethoxy)phosphinyl, or dialkoxy-phosphinyl substituents). Preferred (substituted oxyimino)aryl-30 acetyl groups include those wherein R^ is 2-amino-4-thiazolyl. Also preferred are those groups wherein R^g is methyl, ethyl, carboxy- gc167a -9- methyl, 1-carboxy-l-methylethyl or 2,2,2-tr if luoroethyl (f) (Acylamino)arylacetyl groups having the formula 0 O II II -c-ch-nh-c-r, _ I 17 r14 10 r, wherein r, , is as defined above and r,_ is 14 17 R10 (>tocrii — ^CH2^n-0"' alkylamino, (cyanoalkyl) 15 amino, aminocarbonyl, alkylaminocarbonylt (cyanoalkyl) aminocarbonyl nh nh_ 0 I 2 || -ch-ch„-c-nh-ch. -ch2-nh-c—, 3' 20 hq 02-N(CH2-CH2-0H) 2, 25 , or 30 Preferred (acylamino)arylacetyl qroups of the above formula include those groups wherein Rjy is amino, or amidocarbonyl. Also preferred are those groups wherein R^ is phenyl or 2-thienyl. -10- ncu la 1998 (g) I[[3-Substituted-2-oxo-l-imidazoli-dinyl]carbonyl]amino]arylacetyl groups having the formula 0 0 O c II II -C-CH-NH-C-N. N-R-, Q I ) 18' R14 ch~ Cu2 wherein R^4 is as defined above and is hydrogen, alkylsulfonyl, arylmethyleneamino (i.e., -N=CH-R^4 wherein R^4 is as defined O II above), -C-Ri9 (wherein R^g is hydrogen, alkyl or halogen substituted alkyl), aromatic group (as defined by R^ above), alkyl or substituted alkyl (wherein the alkyl group is substituted with one or more halogen, cyano, nitro, amino or.mercapto groups). Preferred [[3-substituted-2-oxo-l-imidazoli- dinyljcarbonyl]amino]arylacetyl groups of the above formula include those wherein R^ is phenyl or 2-thienyl. Also preferred are those groups wherein R.. 0 is hydrogen, methylsulfonyl, phenyl-J. O methyleneamino or 2-furylmethyleneamino. The term "cation", as used throughout the specification, refers to any positively charged @ 0 atom or group of atoms. The "-SO^M " substituent on the nitrogen atom of the 8-lactams of this invention encompasses all sulfonic acid salts. Pharmaceutically acceptable salts are, of course, preferred, although other salts are also useful in purifying the products of this invention or as intermediates for the preparation 6.7- a -u- (1998 • of pharmaceutically acceptable salts. The cationic portion of the sulfonic acid salts of this invention can be obtained from either organic or inorganic bases. Such cationic portion 5 includes, but is not limited to, the following ions: ammonium; substituted ammonium, such as ( alkylammonium (e.g., tetra-n-butylammonium, referred to hereinafter as tetrabutylaramonium); alkali metal, such as lithium, sodium and potassium; 10 alkaline earth metal, such as calcium and magnesium; pyridinium; dicyclohexylammonium; hydrabaminium; benzathiniura; N-methyl-D-gluca'minium. • As set forth in formula I, and in the 0 definitions following formula I, M can be hydrogen. 15 Such compounds are often referred to in the art as "inner salts" by virtue of a positive and negative charge in the molecule. This invention is directed to those 8-lactams which have been described above, wherein the 20 stereochemistry at the chiral center in the 3-position of the 8-lactam nucleus is the same as the configuration at the carbon atom in the 6-position of naturally occurring penicillins (e.g., penicillin G) and as the configuration 25 at the carbon atom in the 7-position of naturally occurring cephamycins (e.g., cephamycin C). GC16 7 a -12- [1998 2 The S-lactams of formula I have activity against a range of gram-negative and gram-positive organisms. The compounds of this invention can be used as agents to combat bacterial infections (including urinary tract infections and respiratory infections) in mammalian species, such as domesticated animals (e.g., dogs, cats, cows, horses, and the like) and humans. For combating bacterial infections in mammals a compound of this invention can be administered to a mammal in need thereof in an amount pf about 1.4 mg/kg/day to about 350 mg/kg/day, preferably about 14 mg/kg/day to about 100 mg/kg/day. All modes of administration which have been used in the past to deliver penicillins and cephalosporins to the site of the infection are also contemplated for use with the novel B-lactams of this invention. Such methods of administration include oral, intravenous, intramuscular, and as a suppository. The compound of formula I can be prepared from an azetidine having the formula II A, -NH H \ CH C O' ^c- NH wherein A^ is a nitrogen protecting group, e.g., triphenylmethyl, and A2 is an alkyl or GClG7a 15 1998 -13- aryl group. Reaction of an azetidine of formula II with a thiolate having the formula 111 © *2 I ~R3 5 R4 ( yields a mixture of diastereomers of the formulas IVa R2 A1"N\ch 10 | | E4 NH and IVb A -NH F *2 1 VCH CN° R3 I I r4 C NH 0* Separation of the above diastereomers can be accomplished using conventional chromatographic 20 or fractional crystallization techniques. 0 The addition of a sulfo (SO^) group to the 1-position of a compound of formula IVa or IVb or the R=methoxy counterpart yields the corresponding compound having the formula 25 V R2 a R /S-C-R-, A, "NH - , 3 C CH R, I I yC N-S0®M®. CC1G7 a [1998 2 Introduction of the sulfo substituent can be accompli shed by sulfonation as for example by reacting a compound of formula IVa or IVb or the R=methoxy counterpart with a complex of pyridine and sulfur trioxide. The reaction can be run in an organic solvent or in a mixture of organic solvents, preferably a mixture of a polar solvent such as dimethylformamide and a halo-genated hydrocarbon such as dichloromethane. This reaction yields a compound of formula V 0 wherein M is pyridinium ion. Instead of using a pre-formed complex of pyridine and sulfur trioxide, the complex can be formed in situ, e.g., using chlorosulfonyltrimethylsilyl ester and pyridine as reagents. Alternatively, a complex of dimethylformamide-sulfur trioxide, 2-picoline-sulfur trioxide or 2,6-lutidine sulfur trioxide can be used. Using conventional techniques (e.g., ion-exchange resins, crystallization, or ion-pair extraction) the pyridinium salt formed by the above procedure can be converted to other salts. These techniques can also be used to convert the products of formula I, or any of the intermediates described herein, to other salts. I A second method for introducing the sulfo group to the 1-position of an azetidine of formula IVa or IVb comprises first silylating the compound and then subjecting the silated compound to a silyl interchange reaction. Exemplary silylating agents are monosilyltri-fluoroacetamide, trimethylsilylchloride/triethylamine, CC167a -15- 1998 28 and bis-trimethylsilyltrifluoroacetamide, and an exemplary reagent useful for the silyl interchange reaction is trimethylsilyl chloro-sulfonate. 5 Deprotection of an azetidine of formula V yields a zwitterion having the formula VI R2 mu® - yS-C-Ro NH - / | 3 C CH R. I ■' e 10 N-so®. The deprotection techniques used are conventional, and will depend on the particular protecting group (A^) present. Treatment with acid (e.g. , 15 ■ formic acid or trifluoroacetic acid) cleaves a triphenylmethyl or a t-butoxycarbonyl protecting group. Treatment with hydrogen (using a catalyst such as palladium) cleaves a benzyloxycarbonyl protecting group. Treatment with phosgene or 20 phosphorous pentachloride cleaves an amide protecting group. The zwitterions of formula VI are novel intermediates, and as such, constitute an integral part of this invention. Conventional acylation techniques can be 25 used to prepare the products of formula I (wherein R is hydrogen) from a zwitterion of formula VI. Exemplary acylation techniques include reaction with a carboxylic acid (R"^-OH) , or corresponding carboxylic acid 30 halide or carboxylic acid anhydride. The reaction with a carboxylic acid proceeds most readily in 35 C1C16 7 a -16- 1998 the presence of a carbodiimide and a substance capable of forming an active ester in situ such as N-hydroxybenzotriazole. In those instances when the acyl group (R^) contains reactiv-e functionality (such as amino or carboxyl groups) it may be necessary to first protect those functional groups, then carry out the acylation reaction, and finally deprotect the resulting product. Variations of the above described synthetic route (some of which are described in the examples presented below) will be apparent to the practitioner of this invention. For example, a compound of formula IVa or.IVb can be deprotected and then protected with a different amino protecting group before proceeding with the remaining synthetic procedure. The 8-lactams of formula I wherein R is methoxy can be prepared from the corresponding compound of formula I wherein R is hydrogen. Halogenation of the amide nitrogen of a non-methoxylated compound of formula I yields, in situ, an intermediate having the formula VII R, -N C 0 . 0ClG7a -17- 11998 28 Reaction of an intermediate of formula VII with a methoxylating agent, e.g., an alkali metal methoxide yields a product of formula I wherein R is methoxy. The reaction can be 5 run in an organic solvent, e.g., a polar organic solvent such as dimethylformamide, at a reduced temperature. \ An alternative synthesis for preparing the compounds of formula I wherein R is methoxy 10 comprises first alkoxylating a compound of formula IVa or IVb wherein A^NH is a carbamate (e.g. , A^ is benzyloxycarbony.l) and then introducing a sulfo group in the 1-position of the resulting compound. Deprotection and 15 acylation, using the procedures described above, yields the products of formula I wherein R is alkoxy. Alternative syntheses for the preparation of the products of this invention are available, 20 For example, compounds of formula I wherein R^ 0 II is -C-OH can be used as intermediates to obtain Corresponding products of formula I wherein O II 25 R4 is (i) -C-Rg wherein R^ is alkoxy, aryloxy, O II arylalkoxy or (ii) -C-NR^R^. Standard coupling reactions are employed. 30 The following examples are specific embodiments of this invention. GC167 a 199 -18- Example 1 (3R—cis)-[(3-Amino-2—oxo—1—sulfo-4-azetidinyl)thio] acetic acid, methyl ester 5 Method I . A) (3R-cis)-[[2-0x0-3-[(triphenylmethyl) amino]-4-( azetidinyl]thio]acetic acid, methyl ester (3R-cis)-[2-Oxo-3-[(triphenylmethyl)amino]-4-azetidinylkethyl sulfone (20.35 g) 10 is dissolved in 100 ml of acetone and 6.4 g of the sodium salt of inercaptoacetic acid, methyl ester, is added in small portions. After the addition is complete, the solution is stirred for 3 hours at 40°C. The acetone is distilled 15 off and the residue is dissolved in ethyl acetate and extracted three times with brine. The organic layer is dried over Na2S0^ and stripped off. The remaining oil is chromatographed on 400 g of silica, eluting with 9:1 chloroform/ethyl 20 acetatfe, and yielding 7.3 g of the cis product, 6.4 g of the corresponding trans product and 2.6 g of a mixture of the cis and trans products. B) (3R-cis)-[[2-Oxo-l-sulfo-3-[(triphenylmethyl)-25 amino]-4-azetidinyl]thio]acetic acid, methyl ester, tetrabutylammonium salt (3R-cis)-[[2-0x0-3-[(triphenylmethyl)amino]-4-azetidinyl]thio]acetic acid, methyl ester (0.43 g) and 3.6 mmole of a complex of pyridine-30 sulfur trioxide. are dissolved in 10 ml of -19- GC16 7 a 1199 dimethylformamide (dried over molecular sieves), and stirred for 3 hours at 60°C and 8 hours at room temperature. Dimethylformamide is stripped off in vacuo and the oily residue is dissolved in a mixture of 60. ml 0 .5 M potassium phosphate buffer pH 5.5 and 30 ml of dichloroinethane. Tetrabutylammonium hydrogen sulfate (1 irrmole) is added and the organic layer is separated. The aqueous phase is again extracted with 50 ml of dichloromethane. The combined organic layers are dried and dichloromethane is distilled off, yielding 0.6 g of the title compound. • C) (3R-cis)-[(3-Amino-2-oxo-l-sulfo-2-azetidinyl) thio]acetic acid, methyl ester (3R-cis)-[[2-Oxo-l-sulfo-3-[(triphenylmethyl)-amino]-4-azetidinyl]thiojacetic acid, methyl ester, tetrabutylammonium salt (0.3 g) is dissolved in 1 ml of dichloromethane, 8 ml of 9 8%* formic acid is added, and the mixture is stirred for 2 hours. The solution is poured into 30 ml of ether, yielding 0.06 g of the title compound, melting point 10 3-10 5°C, dec. -20- CC167 a 1998 2 Method II A) (3R-cis.) - [ (3-Amino-2-oxo-4-azetidinyl) thio] acetic acid, methyl ester, 4-methylbenzenesulfonate (3R-cis)-[[2-Oxo-3-(triphenylmethyl)amino]-4-azetidinyl]thio]acetic acid, methyl ester (4.33 g) and 1.9 2 g of £-toluenesulfonic acid monohydrate are stirred at 0°C for 3 hours in 50 ml of 1:1 acetone/dichloromethane, yielding 3.55 g of the title compound, melting point 14 6°C, dec. B) (3R-cis)- [ [ (3- [ [ (1,1-Dimethylethoxy)carbonyl]-amino]-2-oxo-4-azetidinylj thio]acetic acid, methyl-ester (3R-cis)-[(3-Amino-2-oxo-4-azetidinyl)thio]-acetic acid, methyl ester, 4-methylbenzenesulfonate (3.63 g) and 3.03 g of triethylamine are dissolved in 30 ml of t-butanol and a solution of 3.28 g of di-t-butylpyrocarbonate in 10 ml of tetra-hydrofuran is added. The mixture is stirred for 8 hours and solvents are distilled off. The oily residue is dissolved in 50 ml of ethyl acetate, which is extracted with aqueous citric acid and water, dried over Na2SC>4 and the ethyl GG10 7a ft998 2 • acetate is stripped off. The oily residue is extracted with petroleum ether and crystallized after chromatography on silica using 6:4 chloroform/ ethyl acetate as eluent, yielding 2.4 g of the 5 title compound, melting point 65-68°C. ». C) (3R-cis) - [ [ (1,1-Dimethylethoxy) carbonyl] amino] - 2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid, methyl ester, tetrabutylammonium salt 10 Following the procedure described in part B of method I, but substituting (3R-cis)-[[(3-[[(1,1-dimethylethoxy) carbonyl] amino]'-2-oxo-4-azetidinyl] -thio]acetic acid, methyl ester for (3R-cis)-[[2-oxo- 3-[[(triphenylmethyl)amino]-4-azetidinyl]thio] 15 acetic acid, methyl ester, yields the title compound as an oil. (3R-cis)-[(3-Amino~2-oxo-l-sulfo-4-azetidinyl)- thio]acetic acid, metnyl ester ! 20 Following the procedure described in part C of method I, but substituting 5.22 g of (3R-cis)-[ [ (1,1-dimethylethoxy)carbonyl]amino]-2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid, methyl ester, tetrabutylammonium salt for (3R-cis)-[[2-oxo-25 l-sulfo-3-t(triphenylmethyl)amino]-4-azetidinyl] -thio]acetic acid, methyl ester, tetrabutylammonium salt, yields 1.90 g of the title compound, m.p. 103-105°C dec. ■CCl67a H9 9 8 -22- Exaimple 2 (3R-trans)-[(3-Amino-2-oxo-l-sulfo-4-azetidinyl)thio] acetic acid, methyl ester A) (3R-trans)-[[2-Oxo-3-[(triphenylmethyl)amino]-4-azetidinyl]thio]acetic acid, methyl ester (3R-cis)-[2-Oxo-3-[(triphenylmethyl)amino]-4-azetidinyl]methyl sulfone (20.35 g) is dissolved in 100 ml of acetone and 6.4 g of the sodium salt of mercaptoace.tic acid, methyl ester, is added in small portions. After the addition is complete, the solution is stirred for 3 hours at 40°C. The acetone is distilled off and the residue is dissolved in ethyl acetate and extracted three times with brine, The organic layer is dried over Na2S0^ and stripped off. The remaining oil is chromatographed on 400 g of silica, eluting with 9:1 chloroform/ethyl acetate, and yielding 6.4 g of the trans product, 7.3 g of the corresponding cis product and 2.6 g of a mixture of the cis and trans products. B) (3R-trans)-[(3-Amino-2-oxo-4-azetidinyl)thio] acetic acid, methyl ester, 4-methylbenzenesulfonate Following the procedure of example 1, part B of method II, but utilizing (3R-trans)-[[2-oxo-3-[(triphenylmethyl)amino]-4-azetidinyl]-thio]acetic acid, methyl ester as the starting material, and utilizing ether to precipitate the product, yields the title compound, melting point 146°C. GC1G7 a -23 199 8 2 C) (3R-txans)-[[(3-[[(1,1-Dimethylethoxy)carbonyl]-amino]-2-oxo-4-azetidinyl]thio]acetic acid, methyl ester Following the procedure of example'1, 5 part C of method II, but utilizing (3R-trans)-[(3-amino-2-oxo-4-azetidinyl).thio]acetic acid, methyl , ester, 4-methylbenzenesulfonate as the starting material, yields the title compound, melting point 81-84°C,. dec. 10 D) (3R-trans)-[[(1,1-Dimethylethoxy)carbonyl]amino]-2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid, methyl ester, tetrabutylammonium salt Following the procedure described in 15 example 1, part B of method I, but substituting (3R-trans)-[[(3-[[(1,1-dimethylethoxy)carbonyl]-amino]-2-oxo-4-azetidinyl]thio]acetic acid, methyl ester for (3R-cis_)-[[ 2-Oxo-3-[[(triphenylmethyl) amino]-4-azetidinyl]thio]acetic acid, methyl 20 esteryields the title compound as an oil. E) (3R-trans)-[(3-Amino-2-oxo-l-sulfo-4-azetidinyl)-thio]acetic acid, methyl ester Following the procedure described in 25 example 1, part C of method I, but utilizing (3R-trans) — [ [ (1,1-dimethylethoxy)carbonyl]amino]-2-oxo-1-sulfo-4-azetidinyl]thio]acetic acid, methyl ester, tetrabutylammonium salt as the starting material, yields the title compound 30 melting point 76-81°C. 35 -24- CC16 7 a 1998 28 * Example 3 [ (3R-cis ) (R*)]-[[3-[f[[( 4-Ethyl-2 , 3 —c3 ioxo-1 -piper -azinyl)carbonyl]amino]phenylacetyl]amino]-2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid; methyl 5 ester,potassium salt (3R-cis)-[(3-Araino-2-oxo-l-sulfo-4-azetidinyl) , thio]acetic acid, methyl ester (542 mg; see example 1), 444 mg of (R)-a-[[(4-ethyl-2,3-dioxo-1-piperazinyl)carbonyl]amino]benzeneacetic acid, 10 202 mg of triethylamine and 350 mg of N-hydroxy-benzotriazole are dissolved in 10 ml of dimethyl-formamide. After cooling in an ice-bath, 412 mg of dicyclohexylcarbodiimide dissolved in 3 ml of tetrahydrofuran is added dropwise while 15 stirring. After 90 minutes the dicyclohexyl-urea which forms is filtered off and dimethyl-formamide is distilled off in_ vacuo (bath temperature 50°C). The remaining oil is dissolved in 30 ml of acetone and 0.7 g of 20 potassium perfluorobutane sulfonate is added, causing 0.7 g of the title compound to precipitate. Purification is performed by column chromatography on HP-20 resin using water and 9:1 water/acetone as eluents. The yield of title 25 compound, melting point 167°C, dec., is 0.27 g after lyophilization. GC16 7a ft 9 9 8 Example 4 [ (3R-trans) (R*)]-[.[3-[[[[(4-Ethyl-2,3-dioxo-l-piperazinyl) carbonyl] aminojphenylacetyl] amino] -2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid, methyl ester, potassium salt Following the procedure described in Example 3 but utilizing (trans)-[(3-amino-2-oxo-l-sulfo-4-azetidinyl)thio]acetic acid, methyl ester as the starting azetidine yields the title compound, melting point 150°C. Example 5 [ (3R-cis) (Z)] — [[3—[ [(2-Amino-4-thiazolyl) (methoxy-imino)acetyl]amino]-2-oxo-l-suIfo-4-azetidinyl]-thio]acetic acid, methyl ester, potassium salt Following the procedure described in example 3, but substituting (Z)-2-amino-a-(methoxyimino)-4-thiazoleacetic acid for (R)-a-[[(4-ethyl-2,3-dioxo-l-piperazinyl)-carbonyl]amino]benzeneacetic acid, yields the title compound, melting point 173°C, dec. Example 6 [(3R-trans) (Z)]-[I 3-[ [(2-Amino-4-thiazolyl)-(methoxyimino)acetyl]amino]-2-oxo-l-sulfo-4-azetidinyl]thiojacetic acid, methyl ester, potassium salt Following the procedure described in example 3, but utilizing (trans)-[(3-amino-2-oxo-l-sulfo-4-azetidinyl)thio]acetic acid, methyl ester as the starting azetidine and ■■GC167 Or 20 25 -26- 1998 2 substituting (Z) -2-amino-ct- (methoxyimino) -4-thiazoleacetic acid for (R)-a-[[(4-ethyl-2,3-dioxo-l-piperazinyl)carbonyl]amino]benzeneacetic acid, yields the title compound, melting -point 15 3°C, dec. < Example 7 [ (3R-cis ) (R*) ] - [ [3- [ [.[ (Aminooxoacetyl) amino] -2-thienylacetyl]amino]-2-oxo-l-sulfo-4-azetidinyl]-10 thio]acetic acid, methyl ester, potassium salt Following the procedure described in example 3, but substituting (R)-a-[ (aminooxoacetyl) amino]-2-thiopheneacetic acid for (R) -a- [ f (4-ethyl-2, S-dioxo-l-^piperazinyl) carbonyl] amino]benzeneacetic acid, yields the title compound, melting point 137°C, dec. Example 8 [(3R-trans) (R*)]-[ [3- [ [[(Aminooxoacetyl)amino]- 2-thienylacetyl]amino]-2-oxo-l-sulfo-4-azetidinyl] thio]acetic acid, methyl ester, potassium salt Following the procedure described in example 3, but utilizing (trans)-[(3-amino-2-oxo-l-sulfo-4-azetidinyl)thio]acetic acid, methyl ester as the starting azetidine and substituting (R)-a-[(aminooxoacetyl)amino]-2-thiopheneacetic acid for (R)-a-[[(4-ethyl-2,3-dioxo-l-piperazinyl)carbonyl]amino]benzeneacetic acid, yields the title compound, melting point 30 158°C, dec. 35 GC?167o fl 9 9 Examples 9-26 Following the procedure of Example 1 (method I), but substituting the compound listed in column I for the sodium salt of mercaptoacetic acid, methyl ester, and the procedure of Example 3, but substituting the acid listed in column II for (R)-a-[[(4-ethyl-2,3-dioxo-l-piperazinyl) carbonyl]amino]benzeneacetic acid, yields the compound listed in column III. Examples 10, 11, 19, 20, 25 and 26 require a deprotection step at the end of the reaction sequence. Deprotection is accomplished in examples 10, 19, 20, 25 and 2 6 by treatment of the protected compound with trifluoroacetic acid and anisole at -15°C. Deprotection is accomplished in example 11 by catalytic (10% palladium on charcoal) hydrogenation of the protected compound. It • Column I Column II 9) Na^S-CH -CH N3C-C11 -S-CIL-C-OH 2 II 2 2 1!) M,'89S-CHn-C-CH,-^7) ^CH-N-IPJ-J-NH-CHJ-OII s 6 • • Column III 0 II NaC-CI^-S-C^-C-NH^ ,s-ch2-cii <y © e W-S03K 8 91! <z) 2 ho-c-c 0-n-c-c-nhn ^s-ch, -C-O S" J> -S 8 J ho-c-ch-c-nh 6 ix? s-ch2-e-ch2ch3 I M 00 I —cn=N-hT^N-^-NH-CM-^-NH^ S-Cll^-CH yr 0 n o >-■ J1 rv vD ■ j fli \0 CO rv) Column I Column II /—\ 9 <R' S 13) K^S-CH -C-oV C",°-Q-CH,-Q-C-NH-C"-^0H s " 6 OH /-A (Z) 2 14) Na S-CH -C-O-CH —CH -0-N=C-C- " nP ee SH3 f ?3 (z) S 15) Na s-iH-c-o-ch (ch3)c-o-c-Ch-o-n=c-c-oh h„its 2 h2c—ch2 -\/ ™ /~~\ S (R) ? 16) Naws-C-c-0-ch2-f^ ch3-ch2-n n-C-nii-Cll-t-oh Cr X) O v| Column III II e$ -C-0 K ch (Z) 0 „-0-N=C-C- s„ C h3 -0-n= C- C- nh ^ ^s-ch2 -^-O-CHj-Q e e ^-so3K 9 9", (2) ft (ch,) c-o-c-ch—0-n=C-c-nh ^s-jlf^-o-ch 3 3 j6 .po/® h2n I to VD I o (R) o ch -cii -l/ ^N-''-NH-CII-c-nii ys-c—c-o-cll • '« i Jl-r n <5 -* <r> 1 s si > vO O 00 N> Column X 17) Na*®S-CH iJ-NH 0 Column II ( v)—cb^n-n n-c-nii-cii—c-oh ^ A Oil CH 18) Na S-ll:H-(j-N (CH^) 2 O ^CH-» ffiQ / 3 19) na s-ch -c-n * o nH 20) Na^S-CII ' o h <Z) 0 CH3CH^—°—H=*C—°H 9 hc ft {Z) ft r0-c-c-c)-n=c-c-' 6 4,3 i h2h (Z) O 9S hc-o-c-ch -o-n=c-c-oh 6 Column III /—V / > II II - u ^vch=n-n n-c-nii-ch-c-nh . s-ch2-l- ....2 w r~\ 0 II (R) 0 II ■n n- -c- •nll-cll- -c h—& A 0 o [ ii V oh y" "" J-lsoy 0 (Z) o CH,CH_-0-N=C-i'-NH-v £ , HjN CIIO S-cii-c-n (ch,) 3 2 0 ® Jl-S03K 2 ../CH] ho-c 0 ?"•» (Z) 9 _l j20-n=c-(!;-nh s-ch2-c-nv 3 j-^S03K,> h2» 0 ft <Z) S S-ch -c-m^ ho-C-ch2"0-n=C-C-nh^ ^ 2 » N'^l J_J-SO®K® I u> 0 1 O »-• sO VO Co Column I Column II CM. 211 »«*9s-cii2-$-h^" ch3-£-S-cii-c-ou '6 0 H ®e >r_\ ft <R) ft 22) Na S-C»2-C=N CH^-N N-C-NH-CH-C-OH /CV- Vf ?"2 Vv ,CU2 9Q^S./ ~ (21 <) 23) Na S-C—C=N CH O-N-C-C-OH 3 d> Column III ch -s-i-ch-^-nf , ..fU /S-CH -C-n 3 ^ N" /—\ ft (R) 9 [ ch_-n n-c-nh-ch-c-nhv .s-ch-on ■"« 6 yt# (Z) o ch.o-n-c-c-nh fa f2 CH2 /CH2 3 6 jXLy s-C—C^N I Co SO Ko 00 N) 00 t • CO OJ K) tO cn o in o (Z) 0 24) Na^S-C^-j^j CH3CH2-0-N=C-C-0H 2 N" H2N OHC-( 0 CH (Z) 0 ffifl M 1 3 II 25) Na S-CH. —i , HC-O-C-C—0-N=C-C-0H CH3 IT ^ h V 0 (z) o ®q n—^ ^ 26) Na S-CH (^Ji HC-0-C-CH2"0-N=C-C-0H i ii h/—* cn cn (z) o II CH CH -0-N=C-C-NH 3 2 n. h2n 0 S-CH e © N-so3K 2~1U) o CH (z) o II I 3 II HO-C-C—0-N=C-C-NH ch3 (I h2N' S-CH e © N-SOK 3 I iU to I 0 (Z) 0 II HO-C- n / s i j c ^ —» - J t> nO O f 00 K) 00 10 ■GC167 -33- 1199828 Example 27 [(3R-cis) (R*)J -[[3 —[[[[(4-Ethyl-2,3-dioxo-l-piperazinyl ) carlponyl] amino] phenylacetyl ] amino] -3-methoxy-2-oxo-l-sulfo-4-azetidinyl]thio] acetic acid, methyl ester, potassium salt To a stirred solution of 0.3 mmole of lithium methoxide in 0.4 ml of dry methanol is added 2.5 ml of dry tetrahydrofuran. This is cooled to -78°C and [(3R-cis)(R*)]-[[3-[[[[(4- ethyl-2,3-dioxo-l-piperazinyl)carbonyl]amino]-phenylacetyl]amino]-2-oxo-l-sulfo-4-azetidinyl]-thio]acetic acid, methyl ester, tetrabutylammonium salt (0.1 mmole; see example 3) in 2 ml of tetra-hydrofuran is added over 10 seconds, followed 15 by 16 li 1 of t-butyl hypochlorite. The mixture is stirred for 15 minutes at -78°C and 25 ml of 0.5 M monobasic potassium phosphate is added. The mixture is warmed to room temperature and the pH is lowered to 5.5 with IN hydrochloric 20 acid." The solvents are removed and the residue is extracted with methanol. Removal of methanol in vacuo gives a residue which is extracted with acetonitrile. Removal of the solvent under reduced pressure yields the 25 title compound. 199 GCl67a -34-Example 28 (3R-cis)-3-Amino-4-[ I (4-chlorophenyl)methyl]thio]-2-oxo-l-azetidinesulfonic acid, inner salt 5 A) (3R-cis)-4-[[(4-Chlorophenyl)methyl]thio]-3- [ (triphenylmethyl)amino]-2-azetidinone and (3R-trans)-4-[ [ (4-chlorophenyl)methyl]thio]-3-[(triphenylmethyl)amino]-2-azetidinone (3R-cis)-I2-Oxo-3-[(triphenylmethyl)amino]-10 4-azetidinyl]methyl sulfone (20.3 g) is dissolved in 50 ml of dry methanol. A solution of 6.5 ml of jD-chlorobenzylmercaptan and 2.8 g of potassium hydroxide in 50 ml of methanol is added dropwise at -5°C; stirring is continued for 1 hour. The 15 solvent is distilled off yielding an oily residue which is dissolved in 200 ml of ethyl acetate and ice water. Citric acid (3 g) is added and stirred for 10 minutes. The organic phase is dried and evaporated yielding 25 g of a crude 20 mixture of the title compound and the corresponding trans isomer as an oil. Separation of the isomers is accomplished using column chromatography on silica, eluting with 9:1 chloroform/ ethyl acetate and yielding 10.5 g of the cis 25 product and 6.5 g of the corresponding trans product as oils. tl 998 GC167a -35- B) (3R-cis)-4-[[(4-Chlorophenyl)methyl]thio]-2-OXO-3-[(triphenylmethyl)amino]-1-azetidine-sulfonic acid, tetrabutylammonium salt Following the procedure of example 29B, but substituting (3R-cis)-4- [ [(4-chlorophenyl)-methyl]thio]-3-[(triphenylmethyl)amino]-2-azetidinone for the corresponding trans isomer, yields the title compound. C) (3R-cis)-3-Amino-4-[[(4-chlorophenyl)methyl]-thio]-2-oxo-l-azetidinesulfonic acid, inner salt (3R-cis)-4-[[(4-Chlorophenyl)methyl]thio]- 2-OXO-3-[(triphenylmethyl)amino]-1-azetidine-sulfonic acid, tetrabutylammonium salt (9.2 g) is dissolved in 20 ml of dichloromethane and 10 ml of formic acid is added and stirred for 30 minutes at 0°C. The solution is then poured into 250 ml of dry ether and 2.8 g of the title compound precipitates out; melting point 8 0°C, dec. Example 29 (3S-trans)-3-Amino-4- [ [(4-chlorophenyl)methyl]-thio]-2-oxo-l-azetidinesulfonic acid, inner salt A) (3R-trans)-4-[[(4-Chlorophenyl)methyl]thio]- 3-[(triphenylmethyl)amino]-2-azetidinone The title compound is prepared as described in example 28A. 1998 ■GC167a m -36- B) (3 R-trans)-4-[[(4-Chlorophenyl)methy1]thio]-2-OXO-3-[(triphenylmethyl)amino]-1-azetidine-sulfonic acid, tetrabutylammonium salt (3R-trans)-4-[[(4-Chlorophenyl)methyl]-thio]-3-[(triphenylmethyl)amino]-2-azetidinone (6.3 g) and 4.17 g of a complex of pyridine and sulfur trioxide are dissolved in 20 ml of dry dimethyIformamide, stirred for 1 hour at 70°C and then for 12 hours at room temperature. The clear solution is then poured into 200 ml of ice water, 4.3 g of tetrabutylammonium hydrogen sulfate is added, and the pH is adjusted to 5.5 with IN potassium hydroxide. The solution is extracted twice with 5 0 ml portions of dichloromethane. From the dried organic phase 10 g of the title compound is obtained as an oil after evaporation. C) (3 R-trans)-3-Amino-4-[[(4-chlorophenyl)-methyl]thio]-2-oxo-l-azetidinesulfonic acid, inner salt Following the procedure of example 28C, but substituting (3R-trans)-4-[[(4-chlorophenyl)-methyl]thio]-2-oxo-3-[(triphenylmethyl)amino]-1-azetidinesulfonic acid, tetrabutylammonium salt for the corresponding cis isomer yields the title compound, melting point 74°C, dec. 119 9 8 2 8 GC167a -37-Example 30 [3r- [3a/ 4 b (z ) ] ] -3- [ [ (2-Amino-4-thiazolyl) -(methoxyimino) ace tyl]amino]-4- [ [ (4-chlorophenyl)-methyl]thio]-2-oxo-l-azetidinesulfonic acid, 5 potassium salt Following the procedure described in example 3, but substituting (3R-trans)-3-amino-4-[[(4-chlorophenyl)methyl]thio]-2-oxo-l-azetidinesulfonic acid, inner salt (see example 29) 10 for (3R-cis) - [ (3-amino-2-oxo-l-sulfo-4-azetidinyl) - thio]acetic acid, methyl ester and (Z)-2-amino-a-(methoxyimino)-4-thiazoleacetic acid for (R) -a- [ [ (4-ethyl-2,3-dioxo-l-piperazinyl)-carbonyl]amino]benzeneacetic acid, yields the 15 title compound, melting point 162°C, dec. Example 31 [3R-[3a,4a(Z)]]-3-[[(2-Amino-4-thiazolyl)(methoxyimino) ace tyl] amino] -4-[[(chlorophenyl)methyl]thio]-20 2-oxo-l-azetidinesulfonic acid, potassium salt Following the procedure described in example 3, but substituting (3R-cis)-3-amino-4-[[(chlorophenyl)methyl]thio]-2-oxo-l-azetidinesulfonic acid, inner salt (see example 28) 25 for (3R-cis)-[(3-amino-2-oxo-l-sulfo-4-azetidinyl)- thio]acetic acid, methyl ester and (Z)-2-amino-a-(methoxyimino)-4-thiazoleacetic acid for (R) -a-[[(4-ethyl-2,3-dioxo-l-piperazinyl)-carbonyl]amino]benzeneacetic acid, yields the 30 title compound, melting point 183-185°C, dec. 10 15 LI 998 2£ GClG7a -38-Example 32 (3R-cis)-3-[[(1,1-Dimethylethoxy)carbonyl]amino]- 2-OXO-4-(methylthio)-1-azetidinesulfonic acid, tetrabutylammonium salt A) (3R-cis)-4-(Methylthio)-3-[(triphenylmethyl)-amino]-2-azetidinone and (3S-trans)-4-(methylthio)- 3- [ (triphenylmethyl)amino-2-azetidinone (3R-cis)-[2-Oxo-3- I (triphenylmethyl)amino]-4-azetidinyl]methyl sulfone (20.4 g) is dissolved in 200 ml of methanol at a temperature of 0°C. A solution of 3.6 g of sodium methylthiolate in 100 ml of methanol is added dropwise. After one hour the reaction mixture is filtered and the solvent is evaporated in vacuo leaving an oily residue which is dissolved in 100 ml of ethyl acetate and extracted twice with 50 ml portions of water. Drying of the organic phase and evaporation yields 29 g of an oily residue which is chromatographed on a 400 g silica column. Elution with 8:2 dichloromethane/ethyl acetate yields 9 g of the cis product, 5 g of the trans product and 7 g of a mixture of the two. 25 * 1998 28 10 GClG7a -39- B) (3R-cis)-3-Araino-4-(methylthio)-2-azetidinone, 4-methylbenzenesulfonate salt (3 R-c is)-4-(Methylthio)-3-[(triphenylmethyl)-amino]-2-azetidinone (10 g) and 5.07 g of 4-methylbenzenesulfonic acid monohydrate are stirred in 500 ml of dry acetone at 5°C for 2 hours. Ethyl ether (50 ml) is added and the title compound precipitates; melting point 145°C, dec. C) (3R-cis)-3-[[(1,1-Dimethylethoxy)carbonyl]-amino]-4-(methylthio)-2-azetidinone (3R-cis)-3-Amino-4-(methylthio)-2-azetidinone, 4-methylbenzenesulfonate salt (6.5 g) is dissolved in 30 ml of t-butanol and 10 ml of tetrahydrofuran cooled to 0°C. Triethylamine (4.3 g) is added and 5.3 g of t-butyl pyrocarbonate is then added dropwise over a 15 minute period. After 3 hours stirring at room temperature, the 20 solvent is distilled off and the residue is dissolved in ethyl acetate, and washed with aqueous citric acid and water. The organic phase is evaporated in vacuo. Crystallization with ether/petroleum ether yields 4 g of the 25 title compound, melting point 112°C, dec. 15 1998 28 CCl67a -40- D) (3R-c_is) -3-[ t (1 / 1-Dimethylethoxy) carbonyl] -amino]-4 -(methylthio)-2-oxo-l-azetidinesulfonic acid, tetrabutylammonium salt (3 R-cis) -3- [ [ (1,1-Dimethylethoxy) carbonyl] -5 amino]-4-(methylthio)-2-azetidinone (220 mg) and 320 mg of a complex of pyridine and sulfur trioxide are dissolved in 15 ml of absolute dimethyIformamide, stirred for 12 hours at room temperature, and poured into 100 ml of 10 ice water. Tetrabutylammonium hydrogen sulfate (340 mg) is added and the pH is adjusted to 5.3 with IN potassium hydroxide. Extraction with dichloromethane yield's 0.43 g of the title compound, melting point 128°C, dec. Example 33 (3R-trans)-3-[[(1,1-Dimethylethoxy)carbonyl]-amino]-2-oxo-4-(methylthio)-1-azetidinesulfonic acid, tetrabutylammonium salt 20 Following the procedure of example 32, but utilizing the trans isomer prepared in part A yields the title compound, melting point 94°C, dec. 11998 GCl67a -41-Example 34 [3R- [3a,4a(Z)]j-3-[[(2-Amino-4-thiazolyl) (methoxyimino) acetyl] amino] -4- (methylthio) -2-oxo-l-azetidinesulfonic acid, potassium salt A) (3R-cis)-4-(Methylthio)-3-[(triphenylmethyl)-amino]-2-oxo-l-azetidinesulfonic acid, tetrabutylammonium salt (3R-cis)-4-(Methylthio)-3-[(triphenylmethyl)-amino]-2-azetidinone (1.87 g; see example 32A) and 1.2 g of a complex of pyridine and sulfur trioxide are dissolved in 15 ml of dimethyl-formamide and heated for 10 minutes to 70°C. The clear solution is then cooled to 5°C, stirred for 4 hours and poured into 100 ml of ice water. Tetrabutylammonium hydrogen sulfate is added and the pH is adjusted to 5.5. Extraction with dichloromethane yields 2.6 g of the title compound as an oil. B) (3R-cis)-3-Amino-4-(methylthio)-2-oxo-1 -azetidinesulfonic acid, inner salt (3R-cis)-4-(Methylthio)-3-[(triphenylmethyl)-amino]-2-oxo-l-azetidinesulfonic acid, tetrabutylammonium salt (2.4 g) is dissolved in 10 ml of dichloromethane and 2.5 ml of formic acid is added. The solution is stirred at -10°C for 30 minutes and poured into 100 ml of ether. Crude product (0.7 g) precipitates and is immediately used in part C. 19 9 8 2 8 CCl67a •• -42- C) [3R-[3ct,4a(Z)]J-3-[[(2-Amino-4-thiazoly1)-(methoxyimino)acetyl]amino]-4-[[(chlorophenyl)-methyl]thio]-2-oxo-l-azetidinesulfonic acid, potassium salt 5 (3R-cis)-3-Amino-4-(methylthio)-2-oxo- l-azetidinesulfonic acid, inner salt (420 mg), 404 mg of (Z)-2-amino-a-(methoxyimino)-4-thiazoleacetic acid, 202 mg of triethylamine and 300 mg of N-hydroxybenzotriazole are 10 dissolved in 10 ml of dimethyIformamide and stirred at -5°C. A solution of 412 mg of dicyclohexylcarbodiimide in 5 ml of tetrahydrofuran is added dropwise. After stirring for 1 hour dicyclohexylurea is filtered off and the 15 solvent is distilled off in vacuo. The oily residue is dissolved in acetone, 680 mg of potassium perfluorobutanesulfonate is added and 530 mg of crude product precipitates. The crude product is chromatographed on HP20, 20 using water as eluent, and yielding 130 mg of the title compound, melting point 193°C,dec. Example 35 [3R-[3a,4B(Z)]]-3-[[(2-Amino-4-thiazolyl)-25 (methoxyimino)acetyl]amino]-4-(methylthio)-2- oxo-l-azetidinesulfonic acid, potassium salt Following the procedure of example 34, but substituting (3R-trans)-4-(methylthio)-3-[(triphenylmethyl)amino]-2-azetidinone (see 30 example 32A) for the corresponding cis isomer, yields the title compound, melting point 195°C, dec. H 9 9 8 2 GCl67a -43- Examples 36 - 40 Following the procedure of Example 34(C) and substituting therein the reactants shown in the table below, the product shown is obtained. In Example 36, the procedure of Example 34(C) is 5 varied by deleting treatment with potassium perfluorobutanesulfonate and instead treating first with trifluoroacetic acid to remove the diphenylmethyl protecting group and then treating with potassium-2-ethylhexaneoate to form the 10 potassium salt. m 00 (N 00 O 0s (p r - v. > r \ U u h2n -cooh ^O^-COOCH 1LN J Cf NH. so, i *3 •N •L. ;-cooh H9r \s' " ocii- h,n cK ^so/ S" >^NH, J ' :h-cooh Nil I co l N'^O h_n j> 0 SO- nh., (:2H5 "2N >conh .9 nh, A ^SO- K (36) \ (/" 0 U \ ^-coo k m.p. 215°(dec.) n ir-C-CO-nh xX H2N *S' A ^ iy' in\s0 k och7 bu3 (37) m.p. 186°C(dec.) :h-conh nh /nh. 0 I (/ ^ _ co so, k I N. ^0 0 (38) ^'2^5 m.p. 191°C(dec.) • • </div>

Claims

<div class="application article clearfix printTableText" id="claims"> 00 <N 00 O o r ■ Vl ) r 1 o u I r ,n \^/-fH-WX)H + H3N nh P >yo ^Ji^O L2H5 CV^ , * s-ol-conh-_j<" z z ^s°5" H2N''^S' >c0-nh ,^s-ch2conh2 Vh30' n -*• ^\ so, (39) m .p. 193°C(dec.) nh- r ;h-c:onh so. nil I co -iV. M (r 'n • 0 C2H5 NH2 -°+ s03 k (40) m .p. 182°C(dec.) aa"a 199938 -46- WHAT ME CLAIM IS:

1. A compound having the formula

R2

R1~NHn^ ^S_<f"R3

C- CH R4

J

o

-N-SO®M®

wherein R is hydrogen or methoxy;

R^ is acyl; _■

R2 and R3 are each independently hydrogen or alkyl, or R2 and R^ when taken together with the carbon atom-to which they are attached form a cycloalkyl group;

R^ is hydrogen, alkyl, aryl, 2,3 or

0 11

4-pyridyl, 2 or 3-furyl/ 2 or 3-thienyl, -C-R^,

0 II

-C-NRgR7 or -C=N;

R^ is hydrogen, alkyl/ aryl, arylalkyl,

hydroxyl, -0eM^ where M® is a cation, alkoxy, aryloxy, or arylalkoxy; R- and R_' are the same or different and ——

6 7 _ r —-

each is hydrogen, alkyl, aryl or arylalkyl; and i M® is . hydrogen or ] a cation •

2. A compound in accordance with claim 1 wherein R is hydrogen.

3. A compound in accordance with claim 2 wherein R4 is -8-R5.

-47-

GCl67a

IWZ29

4. A compound in accordance with claim 3 wherein is hydrogen, alkyl, aryl, or arylalkyl.

5. A compound in accordance with claim 3

wherein R5 is hydroxyl or -cPm* where M® is a cation.

6. A compound in accordance with claim 3 wherein R^ is alkoxy, aryloxy or arylalkoxy.

7. A compound in accordance with claim 2

0 II

wherein R. is -C-NR,R_.

4 6 7

8. A compound in accordance with claim 2

wherein R. is -C=N.

4

9. A compound in accordance with claim 1

0 0-

II II

wherein R. is -C-Rc, -CNRcR_ or -C=N. 4 5 6 7

10. A compound having the formula R ?2

XS~?"R3

l4

VC CH R.

yC— N-SO® M

wherein A^H- is amino or a protected amino group, R is hydrogen or ! methoxy, R,, and R3 are each independently hydrogen or alkyl, or R2 and R3 when taken together with the carbon atom to which they are attached form a cycloalkyl group; j

R4 is hydrogen, alkyl, aryl, 2, 3 or

0 ll

4-pyridyl, 2 or 3-furyl, 2 or 3-thienyl, -C-R^,

0 II

-C-NR6R7, or -C=N;

R^ is hydrogen, alkyl, aryl, arylalkyl,

hydroxyl, alkoxy, aryloxy, or arylalkoxy;

R-. and R_ are the same or different and

D /

each is hydrogen, alkyl, aryl or arylalkyl,and $

M is hydrogen or a cation.

11. A compound according to claim 10 wherein

£1

A-^NH- is amino and- M is hydrogen.

12.

11 wherein R, 4

13.

11 wherein R,

4

14.

11 wherein r,

4

15.

a salt of [(3R-cis)(R*)]-[[3-[[[[(4-ethyl-2,3-dioxo-l-piperazinyl)carbonyl]amino]phenylacetyl]-amino]-2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid, methyl ester.

16. a compound in accordance with claim 1: a salt of [(3R-trans) (R* )] — [[3—[[[£(4-ethyl-

2, 3-dioxo-l-piperazinyl)carbony1]amino]phenyl-acetyl]amino]-2-oxo-1-s ulfo-4-a zetidiny1]thio] acetic acid, methyl ester.

17. A compound in accordance with claim 1:

a salt of [ (3R-cis) (Z)]- [ [3- [ [ (2-amino-4-thiazolyl) -(methoxyimino)acetyl]amino]-2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid, methyl ester.

18. A compound in accordance with claim 1: a salt of [(3R-trans) (Z)]- [ [3-[ [ (2-amino-4-thiazolyl)(methoxyimino)acetyl]amino]-2-oxo-1-sulfo-4-azetidinyl]thio]acetic acid, methyl ester.

19. A compound in accordance with claim 1: a salt of [(3R-cis)(R*)]-[[3-[[[(aminooxoacetyl)-amino]-2-thienylacetyl]amino]-2-oxo-l-sulfo-4-azetidinyl]thio]acetic acid, methyl ester.

-48-

A compound in accordance

0 II

is -C-R,..

o

A compound in accordance

0 II

is -C-NRcR_.

D /

A compound in accordance is —C=N.

A compound in accordance

GC167a

IQZSZ8

with claim 10 or with claim. 10 or with claim 10 or with claim 1:

vU

*

GC167a mm

-49-

20. A compound in accordance with claim 1:

a salt of [(3R-trans)(R*)]-[[3-[[[(aminooxoacetyl)-amino]-2-thienylacetyl]amino]-2-oxo-1-sulfo-4-azetidinyl]thio]acetic acid, methyl ester.

21. A compound in accordance with claim 1 having the structure

^-C00~K+

22. A compound in accordance with claim 1 having the structure

23. A compound in accordance with claim 1 having the structure

24. A compound in accordance with claim 1 having the structure

2

3

t

GC167a inms

-50-

25. A compound in accordance with claim 1 having the structure

(R) I

-p-CONH.

NH I

CO

fV°

c2H5

K -°+

S03 K

DATED THIS |^ DAY A. J. PARK & SOW PER

AGENTS FOR THE APPLICANTS

Reference has been directed, in pursuance of Section 14 of the Patents Act 1953,/to specification No.199168.

H. BURTON Assistant Commissioner of Patents

27 /1/1987

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