NO930622L - PROMOTE PROBE VECTORS, AID TO AA REPLICATORS IN E.COLI, B.SUBTILIS, LACTOCOCCI AND LACTOBACELLUS, AND USE THEREOF - Google Patents

PROMOTE PROBE VECTORS, AID TO AA REPLICATORS IN E.COLI, B.SUBTILIS, LACTOCOCCI AND LACTOBACELLUS, AND USE THEREOF

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Publication number
NO930622L
NO930622L NO93930622A NO930622A NO930622L NO 930622 L NO930622 L NO 930622L NO 93930622 A NO93930622 A NO 93930622A NO 930622 A NO930622 A NO 930622A NO 930622 L NO930622 L NO 930622L
Authority
NO
Norway
Prior art keywords
vectors
lactococci
subtilis
coli
lactobacellus
Prior art date
Application number
NO93930622A
Other languages
Norwegian (no)
Other versions
NO930622D0 (en
Inventor
Ilkka Palva
Mervi Sibakov
Teija Koivula
Atte Von Wright
Original Assignee
Valio Finnish Co Operative Dai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valio Finnish Co Operative Dai filed Critical Valio Finnish Co Operative Dai
Publication of NO930622D0 publication Critical patent/NO930622D0/en
Publication of NO930622L publication Critical patent/NO930622L/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

DNA sekvenser, avledet fra Lactococcus lactis subsp. lactis er nyttige som promotere og promoter/sekresjonsaktiverende signaler for heterolog eller homolog uttrykking i gram- positive bakterier. Ved et ytterligere aspekt vedrører oppfinnelsen vektorer, som plasmider, omfattende sekvensene i samsvar med den foreliggende oppfinnelse, og vertceller transformert med slike vektorer. Ytterligere aspekter av den foreliggende oppfinnelse vedrører metoder for fremstilling av ønskede heterologe eller homologe peptider eller proteiner . under anvendelse av sekvensene, vektorene eller transformerte celler i samsvar med oppfinnelsen. Ved hjelp av oppfinnelsen kan det oppnås sterkt forbedret heterolog og homolog uttrykking og sekresjon i E. coli og i gram-positive bakterier som B. subtilis, Lactococci og Lactobacillus.DNA sequences derived from Lactococcus lactis subsp. lactis are useful as promoters and promoter / secretion activating signals for heterologous or homologous expression in gram-positive bacteria. In a further aspect, the invention relates to vectors, such as plasmids, comprising the sequences of the present invention, and host cells transformed with such vectors. Further aspects of the present invention relate to methods for preparing desired heterologous or homologous peptides or proteins. using the sequences, vectors or transformed cells according to the invention. By means of the invention, greatly improved heterologous and homologous expression and secretion in E. coli and in gram-positive bacteria such as B. subtilis, Lactococci and Lactobacillus can be obtained.

NO93930622A 1990-08-30 1993-02-23 PROMOTE PROBE VECTORS, AID TO AA REPLICATORS IN E.COLI, B.SUBTILIS, LACTOCOCCI AND LACTOBACELLUS, AND USE THEREOF NO930622L (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/FI1990/000204 WO1992004451A1 (en) 1990-08-30 1990-08-30 Promoter probe vectors, able to replicate in e.coli, b.subtilis, lactococci and lactobacillus as well as uses thereof

Publications (2)

Publication Number Publication Date
NO930622D0 NO930622D0 (en) 1993-02-23
NO930622L true NO930622L (en) 1993-04-28

Family

ID=8556494

Family Applications (1)

Application Number Title Priority Date Filing Date
NO93930622A NO930622L (en) 1990-08-30 1993-02-23 PROMOTE PROBE VECTORS, AID TO AA REPLICATORS IN E.COLI, B.SUBTILIS, LACTOCOCCI AND LACTOBACELLUS, AND USE THEREOF

Country Status (5)

Country Link
EP (1) EP0550428A1 (en)
JP (1) JPH06503945A (en)
AU (1) AU652920B2 (en)
NO (1) NO930622L (en)
WO (1) WO1992004451A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242821A (en) * 1989-07-10 1993-09-07 Valio, Finnish Co-Operative Dairies' Association Lactococcus promoter and signal sequences for expression in bacteria
AU4329793A (en) * 1992-06-30 1994-01-24 Viagen Oy (lactobacillus) expression system using surface protein gene sequences
US5837509A (en) * 1992-12-30 1998-11-17 Bioteknologisk Institut Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
EP0677110B2 (en) * 1992-12-30 2010-01-27 Bioneer A/S Recombinant lactic acid bacterium containing an inserted promoter
WO1995031563A1 (en) * 1994-05-12 1995-11-23 Quest International B.V. Complex inducible promoter system derivable from a phage of a lactic acid bacterium (lab), and its use in a lab for production of a desired protein
NL9401935A (en) * 1994-11-18 1996-07-01 Nl Zuivelonderzoek Inst Method for controlling the gene expression in lactic acid bacteria
DK0712935T3 (en) * 1994-11-18 2002-07-15 Stichting Nl I Voor Zuivelonde Method for controlling gene expression in lactic acid bacteria
WO1996032486A1 (en) * 1995-04-11 1996-10-17 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Method for the construction of vectors for lactic acid bacteria like lactobacillus such that the bacteria can efficiently express, secrete and display proteins at the surface
FR2739629B1 (en) * 1995-10-06 1997-12-26 Systems Bio Ind USE OF A DRY-DEPENDENT SECRETION SYSTEM FOR SECRETING PROTEIN NORMALLY SECRETED BY A DRY-INDEPENDENT SECRETION SYSTEM, BACTERIA CONTAINING THEM AND THEIR USE
US6133023A (en) * 1996-09-06 2000-10-17 Bioteknologisk Institut Lactic acid bacterial regulatable expression system
ATE351912T1 (en) * 1999-08-06 2007-02-15 Bioneer As SIGNAL PEPTIDES FROM LACTOCOCCUS LACTIS
EP1239032A1 (en) 2001-03-02 2002-09-11 Société des Produits Nestlé S.A. Lactic acid bacteria as agents for treating and preventing allergy

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4725535A (en) * 1983-02-22 1988-02-16 Sonenshein Abraham L Promoter probe vectors

Also Published As

Publication number Publication date
NO930622D0 (en) 1993-02-23
EP0550428A1 (en) 1993-07-14
AU6277490A (en) 1992-03-30
JPH06503945A (en) 1994-05-12
WO1992004451A1 (en) 1992-03-19
AU652920B2 (en) 1994-09-15

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