NO884847L - Polykationiske baerere for rensing av nukleinsyrer. - Google Patents
Polykationiske baerere for rensing av nukleinsyrer.Info
- Publication number
- NO884847L NO884847L NO884847A NO884847A NO884847L NO 884847 L NO884847 L NO 884847L NO 884847 A NO884847 A NO 884847A NO 884847 A NO884847 A NO 884847A NO 884847 L NO884847 L NO 884847L
- Authority
- NO
- Norway
- Prior art keywords
- stated
- probe
- polynucleotides
- particles
- separation
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract description 23
- 102000039446 nucleic acids Human genes 0.000 title abstract description 20
- 108020004707 nucleic acids Proteins 0.000 title abstract description 20
- 239000000969 carrier Substances 0.000 title description 11
- 238000004140 cleaning Methods 0.000 title 1
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- 238000000034 method Methods 0.000 claims abstract description 172
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- 239000002773 nucleotide Substances 0.000 claims abstract description 129
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 129
- 239000007787 solid Substances 0.000 claims abstract description 101
- 238000000926 separation method Methods 0.000 claims abstract description 85
- 230000027455 binding Effects 0.000 claims abstract description 45
- 238000003556 assay Methods 0.000 claims abstract description 44
- 238000000746 purification Methods 0.000 claims abstract description 26
- 239000000356 contaminant Substances 0.000 claims abstract description 17
- 239000000243 solution Substances 0.000 claims description 165
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- 239000002245 particle Substances 0.000 claims description 39
- 125000002091 cationic group Chemical group 0.000 claims description 37
- 238000001514 detection method Methods 0.000 claims description 31
- -1 phosphate diesters Chemical class 0.000 claims description 27
- 238000010828 elution Methods 0.000 claims description 26
- 239000011521 glass Substances 0.000 claims description 16
- 229910019142 PO4 Inorganic materials 0.000 claims description 15
- 239000010452 phosphate Substances 0.000 claims description 15
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- 229920000151 polyglycol Polymers 0.000 claims description 11
- 239000010695 polyglycol Substances 0.000 claims description 11
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- 239000005017 polysaccharide Substances 0.000 claims description 11
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims description 10
- 239000004952 Polyamide Substances 0.000 claims description 10
- 230000015572 biosynthetic process Effects 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 10
- 229920002647 polyamide Polymers 0.000 claims description 10
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical group CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 6
- 150000004982 aromatic amines Chemical class 0.000 claims description 5
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- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 4
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims description 4
- 239000000467 phytic acid Substances 0.000 claims description 4
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- 235000019832 sodium triphosphate Nutrition 0.000 claims description 4
- UNXRWKVEANCORM-UHFFFAOYSA-I triphosphate(5-) Chemical compound [O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O UNXRWKVEANCORM-UHFFFAOYSA-I 0.000 claims description 4
- 125000000217 alkyl group Chemical group 0.000 claims description 2
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- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 32
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- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 23
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- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 15
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- MBYLVOKEDDQJDY-UHFFFAOYSA-N tris(2-aminoethyl)amine Chemical compound NCCN(CCN)CCN MBYLVOKEDDQJDY-UHFFFAOYSA-N 0.000 description 11
- NNARPPSIYMCXMB-UHFFFAOYSA-N 1,4-bis(2-methylpropoxy)-1,4-dioxobutane-2-sulfonic acid Chemical compound CC(C)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(C)C NNARPPSIYMCXMB-UHFFFAOYSA-N 0.000 description 10
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
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- QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 description 7
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- FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 description 7
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- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 6
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Microbiology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Cleaning Or Drying Semiconductors (AREA)
- Detergent Compositions (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
- Cleaning And De-Greasing Of Metallic Materials By Chemical Methods (AREA)
- Extraction Or Liquid Replacement (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2086687A | 1987-03-02 | 1987-03-02 | |
PCT/US1988/000550 WO1988006633A1 (en) | 1987-03-02 | 1988-03-02 | Polycationic supports for nucleic acid purification, separation and hybridization |
Publications (2)
Publication Number | Publication Date |
---|---|
NO884847D0 NO884847D0 (no) | 1988-10-31 |
NO884847L true NO884847L (no) | 1988-12-22 |
Family
ID=21801014
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO884847A NO884847L (no) | 1987-03-02 | 1988-10-31 | Polykationiske baerere for rensing av nukleinsyrer. |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0281390B1 (de) |
JP (1) | JP2862547B2 (de) |
KR (1) | KR960000479B1 (de) |
AT (1) | ATE107654T1 (de) |
CA (1) | CA1339446C (de) |
DE (1) | DE3850273T2 (de) |
DK (1) | DK607588A (de) |
ES (1) | ES2054797T3 (de) |
FI (1) | FI885022A (de) |
NO (1) | NO884847L (de) |
PT (1) | PT86881A (de) |
WO (1) | WO1988006633A1 (de) |
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NL8203102A (nl) * | 1982-08-04 | 1984-03-01 | Katholieke Universiteit Facult | Papier voor rna-blotting; werkwijze voor het maken daarvan; toepassingen van het papier. |
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DE3650349T2 (de) * | 1985-03-15 | 1995-12-14 | Antivirals Inc | Immunotestmittel für polynukleotid und verfahren. |
US4806546A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on derivatized nylon supports |
US4806631A (en) * | 1985-09-30 | 1989-02-21 | Miles Inc. | Immobilization of nucleic acids on solvolyzed nylon supports |
-
1988
- 1988-03-02 CA CA000560293A patent/CA1339446C/en not_active Expired - Lifetime
- 1988-03-02 WO PCT/US1988/000550 patent/WO1988006633A1/en active Application Filing
- 1988-03-02 EP EP88301839A patent/EP0281390B1/de not_active Expired - Lifetime
- 1988-03-02 DE DE3850273T patent/DE3850273T2/de not_active Expired - Lifetime
- 1988-03-02 JP JP63502502A patent/JP2862547B2/ja not_active Expired - Fee Related
- 1988-03-02 ES ES88301839T patent/ES2054797T3/es not_active Expired - Lifetime
- 1988-03-02 PT PT86881A patent/PT86881A/pt not_active Application Discontinuation
- 1988-03-02 AT AT88301839T patent/ATE107654T1/de not_active IP Right Cessation
- 1988-03-02 KR KR1019880701387A patent/KR960000479B1/ko not_active IP Right Cessation
- 1988-10-31 NO NO884847A patent/NO884847L/no unknown
- 1988-11-01 FI FI885022A patent/FI885022A/fi not_active IP Right Cessation
- 1988-11-01 DK DK607588A patent/DK607588A/da not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
KR960000479B1 (ko) | 1996-01-08 |
AU1426988A (en) | 1988-09-26 |
EP0281390B1 (de) | 1994-06-22 |
DK607588A (da) | 1988-12-28 |
FI885022A0 (fi) | 1988-11-01 |
EP0281390A3 (en) | 1990-04-25 |
AU618951B2 (en) | 1992-01-16 |
EP0281390A2 (de) | 1988-09-07 |
DE3850273D1 (de) | 1994-07-28 |
JPH01502319A (ja) | 1989-08-17 |
WO1988006633A1 (en) | 1988-09-07 |
PT86881A (pt) | 1989-03-30 |
CA1339446C (en) | 1997-09-09 |
ATE107654T1 (de) | 1994-07-15 |
NO884847D0 (no) | 1988-10-31 |
ES2054797T3 (es) | 1994-08-16 |
FI885022A (fi) | 1988-11-01 |
DE3850273T2 (de) | 1994-09-29 |
DK607588D0 (da) | 1988-11-01 |
KR890700682A (ko) | 1989-04-26 |
JP2862547B2 (ja) | 1999-03-03 |
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