NO861551L - CHANGE OF THE DNA SEQUENCE BETWEEN SHINE-DALGARNO SEQUENCE AND STARTING CODON OF THE TRP OPERON TO INCREASE PROTEIN EXPRESSION. - Google Patents

CHANGE OF THE DNA SEQUENCE BETWEEN SHINE-DALGARNO SEQUENCE AND STARTING CODON OF THE TRP OPERON TO INCREASE PROTEIN EXPRESSION.

Info

Publication number
NO861551L
NO861551L NO86861551A NO861551A NO861551L NO 861551 L NO861551 L NO 861551L NO 86861551 A NO86861551 A NO 86861551A NO 861551 A NO861551 A NO 861551A NO 861551 L NO861551 L NO 861551L
Authority
NO
Norway
Prior art keywords
sequence
dna
ligation
shine
coli
Prior art date
Application number
NO86861551A
Other languages
Norwegian (no)
Other versions
NO175646B (en
NO175646C (en
Inventor
Joachim Engels
Michael Leineweber
Eugen Uhlmann
Friedrich Wengenmayer
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of NO861551L publication Critical patent/NO861551L/en
Publication of NO175646B publication Critical patent/NO175646B/no
Publication of NO175646C publication Critical patent/NO175646C/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)

Abstract

For the contracting states : BE, CH, DE, GB, IT, LI, LU, NL, SE 1. DNA segment of the trp operon from E. coli, characterized by the sequence (coding strand) I 5' CTATCGACC 3' (I) which is connected at the 5' end to the Shine-Dalgarno sequence AAGG, and at the 3' end to the start codon ATG. For the contracting state : AT 1. A process for the preparation of a vector having the trp expression system of E. coli, characterized by cleavage with the restriction enzyme Nco l of an E. coli vector which contains the DNA sequence I 5' GTATCGACC 3' (I) (coding strand) followed by 5' ATGG 3', and a) ligation of a gene structure of DNA sequence II 5' CATG X... 3' 3' Y... 5' (II) in which X and Y denote the first complementary pair of nucleotides downstream of the start codon of a structural gene, in the cleavage site, or b) enzymatic filling in of the cleavage site and ligation of the DNA of the sequence III 5' GTA TCG ACC ATG 3' (III) 3' CAT AGC TGG TAC 5' with a DNA of the sequence IV 5' X... 3' (IV) 3' Y... 5' in which X and Y have the abovementioned meaning, or c) enzymatic degradation of the protruding sequence of the cleavage site, and ligation of the DNA of the sequence VI 5' GTA TCG AC 3' (VI) 3' CAT AGC TG 5' with a DNA of the sequence VII 5' Z ATG X... 3' (VII) 3' Z'TAC Y... 5' Z and Z' denoting any desired pair of nucleotides, which can also be dispensed with.
NO861551A 1985-04-19 1986-04-18 Modification of the DNA sequence between the Shine-Dalgarno sequence and the start codon of the trp operon to increase protein expression, method of producing a vector with the trp expression system from E. coli, expression vector NO175646C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853514113 DE3514113A1 (en) 1985-04-19 1985-04-19 CHANGE OF THE DNA SEQUENCE BETWEEN SHINE-DALGARNO SEQUENCE AND START CODON OF THE TRP OPERON TO INCREASE PROTEIN EXPRESSION

Publications (3)

Publication Number Publication Date
NO861551L true NO861551L (en) 1986-10-20
NO175646B NO175646B (en) 1994-08-01
NO175646C NO175646C (en) 1994-11-09

Family

ID=6268543

Family Applications (1)

Application Number Title Priority Date Filing Date
NO861551A NO175646C (en) 1985-04-19 1986-04-18 Modification of the DNA sequence between the Shine-Dalgarno sequence and the start codon of the trp operon to increase protein expression, method of producing a vector with the trp expression system from E. coli, expression vector

Country Status (19)

Country Link
EP (1) EP0198415B1 (en)
JP (1) JPH0665317B2 (en)
KR (1) KR940004543B1 (en)
AT (1) ATE44046T1 (en)
AU (1) AU600229B2 (en)
CA (1) CA1321963C (en)
DE (2) DE3514113A1 (en)
DK (1) DK172695B1 (en)
ES (2) ES8704541A1 (en)
FI (1) FI84362C (en)
GR (1) GR861022B (en)
HU (1) HU196458B (en)
IE (1) IE58994B1 (en)
IL (1) IL78529A (en)
NO (1) NO175646C (en)
NZ (1) NZ215858A (en)
PH (1) PH26596A (en)
PT (1) PT82417B (en)
ZA (1) ZA862925B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966849A (en) * 1985-09-20 1990-10-30 President And Fellows Of Harvard College CDNA and genes for human angiogenin (angiogenesis factor) and methods of expression
AU8379991A (en) * 1990-09-14 1992-03-26 Astra Aktiebolag A novel method of generating clones for the expression of unfused proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3247922A1 (en) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim DNA SEQUENCES, THEIR PRODUCTION, PLASMIDES CONTAINING THESE SEQUENCES AND THE USE THEREOF FOR THE SYNTHESIS OF EUKARYOTIC GENE PRODUCTS IN PROKARYOTS
JPS60188077A (en) * 1984-03-09 1985-09-25 Teruhiko Beppu Novel manifestation plasmid having whole sequence of calf prochymosin cdna
DE3430683A1 (en) * 1984-08-21 1986-03-06 Hoechst Ag, 6230 Frankfurt SYNTHETIC REGULATION REGION

Also Published As

Publication number Publication date
NO175646B (en) 1994-08-01
DE3663956D1 (en) 1989-07-20
IE861031L (en) 1986-10-19
KR940004543B1 (en) 1994-05-25
FI861624A0 (en) 1986-04-17
FI861624A (en) 1986-10-20
PH26596A (en) 1992-08-19
IL78529A0 (en) 1986-08-31
DK179686A (en) 1986-10-20
HUT41068A (en) 1987-03-30
ATE44046T1 (en) 1989-06-15
NO175646C (en) 1994-11-09
IE58994B1 (en) 1993-12-15
NZ215858A (en) 1989-01-06
ES554097A0 (en) 1987-04-01
PT82417B (en) 1988-08-17
AU5638786A (en) 1986-10-23
JPH0665317B2 (en) 1994-08-24
ES8704541A1 (en) 1987-04-01
EP0198415A2 (en) 1986-10-22
DE3514113A1 (en) 1986-10-23
KR860008286A (en) 1986-11-14
DK179686D0 (en) 1986-04-18
ES556233A0 (en) 1987-07-01
GR861022B (en) 1986-08-18
HU196458B (en) 1988-11-28
PT82417A (en) 1986-05-01
JPS61242583A (en) 1986-10-28
EP0198415B1 (en) 1989-06-14
EP0198415A3 (en) 1986-12-30
FI84362C (en) 1991-11-25
ES8706818A1 (en) 1987-07-01
ZA862925B (en) 1986-12-30
AU600229B2 (en) 1990-08-09
FI84362B (en) 1991-08-15
IL78529A (en) 1991-07-18
DK172695B1 (en) 1999-05-31
CA1321963C (en) 1993-09-07

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