NO20061189L - Materialer og fremgangsmater for innfanging av patogener og fjerning av aurintrikarboksylsyre fra en prove - Google Patents

Materialer og fremgangsmater for innfanging av patogener og fjerning av aurintrikarboksylsyre fra en prove

Info

Publication number
NO20061189L
NO20061189L NO20061189A NO20061189A NO20061189L NO 20061189 L NO20061189 L NO 20061189L NO 20061189 A NO20061189 A NO 20061189A NO 20061189 A NO20061189 A NO 20061189A NO 20061189 L NO20061189 L NO 20061189L
Authority
NO
Norway
Prior art keywords
sample
materials
methods
aurintricarboxylic acid
pathogens
Prior art date
Application number
NO20061189A
Other languages
English (en)
Inventor
Matt Ewert
Phil Amuso
Andrew Cannons
Original Assignee
Univ South Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/604,779 external-priority patent/US7998699B2/en
Application filed by Univ South Florida filed Critical Univ South Florida
Publication of NO20061189L publication Critical patent/NO20061189L/no

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/10—Peptides being immobilised on, or in, an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C65/00—Compounds having carboxyl groups bound to carbon atoms of six—membered aromatic rings and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups
    • C07C65/32—Compounds having carboxyl groups bound to carbon atoms of six—membered aromatic rings and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups containing keto groups
    • C07C65/40—Compounds having carboxyl groups bound to carbon atoms of six—membered aromatic rings and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups containing keto groups containing singly bound oxygen-containing groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Sustainable Development (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Foreliggende oppfinnelse vedrører fremgangsmåter og materialer for å ekstrahere smittsomme patogener fra en prøve, slik som blod, som omfatter trinnet med å danne et fibrinaggregat som omfavner patogenet og introdusere et fibrinlysisreagens for å eksponere patogenet for analyse. Fibrinlysisreagenset er fortrinnsvis sammensatt av plasminogen og streptokinase frosset sammen inntil fibrinlysisreagens er nødvendig, hvorved streptokinase enzymatisk reagerer med plasminogen for å danne plasmin ved opptining. Fortrinnsvis er plasminogenet suspendert i en vandig saltløsning før innfrysning, inkludert NaCl og Na3PO4. Foreliggende oppfinnelse vedrører også materialer og fremgangsmåte for effektivt å fjerne ATA fra en prøve som omfatter nukleinsyrepreparat. Foreliggende fremgangsmåte tilveiebringer et nukleinsyrepreparat som er tilstrekkelig fritt for ATA, slik at en RT-PCR-reaksjon og andre reaksjoner som involverer reverstranskriptase kan bli utført.
NO20061189A 2003-08-15 2006-03-14 Materialer og fremgangsmater for innfanging av patogener og fjerning av aurintrikarboksylsyre fra en prove NO20061189L (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/604,779 US7998699B2 (en) 2002-08-15 2003-08-15 Early detection of pathogens in blood
US48189204P 2004-01-14 2004-01-14
PCT/US2004/026606 WO2005021799A2 (en) 2003-08-15 2004-08-16 Materials and methods for capture of pathogens and removal of aurintricarboxylic acid from a sample

Publications (1)

Publication Number Publication Date
NO20061189L true NO20061189L (no) 2006-04-26

Family

ID=34840999

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20061189A NO20061189L (no) 2003-08-15 2006-03-14 Materialer og fremgangsmater for innfanging av patogener og fjerning av aurintrikarboksylsyre fra en prove

Country Status (13)

Country Link
US (12) US7416843B2 (no)
EP (3) EP2103694B1 (no)
JP (2) JP4662932B2 (no)
AT (2) ATE510931T1 (no)
AU (2) AU2004269340B2 (no)
DE (1) DE602004020999D1 (no)
DK (2) DK1654387T3 (no)
ES (1) ES2325445T3 (no)
NO (1) NO20061189L (no)
PL (2) PL1654387T3 (no)
PT (2) PT2103694E (no)
SI (1) SI1654387T1 (no)
WO (1) WO2005021799A2 (no)

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Also Published As

Publication number Publication date
US20130130271A1 (en) 2013-05-23
US8759479B2 (en) 2014-06-24
US8815502B2 (en) 2014-08-26
EP2103694B1 (en) 2011-05-25
JP5253451B2 (ja) 2013-07-31
US20120164714A1 (en) 2012-06-28
WO2005021799A2 (en) 2005-03-10
PL1654387T3 (pl) 2009-10-30
US20130130234A1 (en) 2013-05-23
JP4662932B2 (ja) 2011-03-30
US8916698B2 (en) 2014-12-23
US20130236920A1 (en) 2013-09-12
US20140142047A1 (en) 2014-05-22
US8785176B2 (en) 2014-07-22
ES2325445T3 (es) 2009-09-04
DK2103694T3 (da) 2011-09-12
DK1654387T3 (da) 2009-06-22
US20130122509A1 (en) 2013-05-16
AU2010207760B2 (en) 2012-06-07
US20130288342A1 (en) 2013-10-31
US20130109885A1 (en) 2013-05-02
US8574866B2 (en) 2013-11-05
PT2103694E (pt) 2011-07-26
EP2112232B1 (en) 2014-04-23
DE602004020999D1 (de) 2009-06-18
PL2103694T3 (pl) 2011-10-31
US7416843B2 (en) 2008-08-26
AU2004269340A1 (en) 2005-03-10
ATE510931T1 (de) 2011-06-15
AU2004269340B2 (en) 2010-09-16
US8592200B2 (en) 2013-11-26
EP2103694A1 (en) 2009-09-23
US8962243B2 (en) 2015-02-24
US8927243B2 (en) 2015-01-06
WO2005021799A3 (en) 2005-08-18
US20140093944A1 (en) 2014-04-03
JP2007502618A (ja) 2007-02-15
EP1654387A2 (en) 2006-05-10
EP2112232A1 (en) 2009-10-28
EP1654387B1 (en) 2009-05-06
SI1654387T1 (sl) 2009-10-31
US8465966B2 (en) 2013-06-18
ATE430812T1 (de) 2009-05-15
PT1654387E (pt) 2009-07-20
US20130130302A1 (en) 2013-05-23
US8735090B2 (en) 2014-05-27
US20050277130A1 (en) 2005-12-15
US20130115687A1 (en) 2013-05-09
AU2010207760A1 (en) 2010-09-02
JP2010193901A (ja) 2010-09-09

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