NO20050410L - Proteinglykosyleringsmodifisering i Pichia Pastoris - Google Patents

Proteinglykosyleringsmodifisering i Pichia Pastoris

Info

Publication number
NO20050410L
NO20050410L NO20050410A NO20050410A NO20050410L NO 20050410 L NO20050410 L NO 20050410L NO 20050410 A NO20050410 A NO 20050410A NO 20050410 A NO20050410 A NO 20050410A NO 20050410 L NO20050410 L NO 20050410L
Authority
NO
Norway
Prior art keywords
pichia pastoris
pichia
protein glycosylation
glycosylation modification
mannosidase
Prior art date
Application number
NO20050410A
Other languages
English (en)
Inventor
Roland Henri Contreras
Nico L M Callewaert
Steven C J Geysens
Vladimir Kaigorodov
Vervecken Wouter
Original Assignee
Flanders Interuniversity Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Flanders Interuniversity Inst filed Critical Flanders Interuniversity Inst
Publication of NO20050410L publication Critical patent/NO20050410L/no

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01024—Alpha-mannosidase (3.2.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01114—Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase (3.2.1.114)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

172574-HJ 2005-03-23 Sammendrag Foreliggende oppfinnelse tilveiebringer genetisk fremstilte metylotrofe gjærstammer inkludert Pichia, og spesielt Pichia pastoris, som er i stand til å produsere proteiner med redusert eller modifisert glykosylering. Fremgangsmåter for fremstilling av glykoproteiner med redusert og/eller modifisert glykosylering ved å benytte slike genetisk fremstilte stammer av Pichia blir også tilveiebrakt. Vektorer som omfatter kodende sekvenser for (-1,2-mannosidase I, glukosidase II, GlcNAc-transferase I og mannosidase II eller som omfatter OCH1-oppbrytende sekvens for transformering av metylotrof gjær, er omfattet av foreliggende oppfinnelse. Sett som inneholder vektorene er også inkludert i denne oppfinnelsen.
NO20050410A 2002-06-26 2005-01-25 Proteinglykosyleringsmodifisering i Pichia Pastoris NO20050410L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/185,475 US7252933B2 (en) 2002-06-26 2002-06-26 Protein glycosylation modification in methylotrophic yeast
PCT/EP2003/006711 WO2004003194A2 (en) 2002-06-26 2003-06-25 Protein glycosylation modification in pichia pastoris

Publications (1)

Publication Number Publication Date
NO20050410L true NO20050410L (no) 2005-03-23

Family

ID=29999263

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20050410A NO20050410L (no) 2002-06-26 2005-01-25 Proteinglykosyleringsmodifisering i Pichia Pastoris

Country Status (10)

Country Link
US (2) US7252933B2 (no)
EP (2) EP2302047A1 (no)
JP (1) JP2006502705A (no)
KR (1) KR101047167B1 (no)
AU (1) AU2003238051B2 (no)
CA (1) CA2490268C (no)
IL (2) IL165717A0 (no)
MX (1) MXPA04012983A (no)
NO (1) NO20050410L (no)
WO (1) WO2004003194A2 (no)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
EP2267135A3 (en) 2000-06-30 2011-09-14 Vib Vzw Protein glycosylation modification in pichia pastoris
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
US7507573B2 (en) * 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
WO2006026992A1 (en) * 2004-09-07 2006-03-16 Novozymes A/S Altered structure of n-glycans in a fungus
US8202523B2 (en) * 2005-09-22 2012-06-19 ProSci, Inc. Glycosylated polypeptides produced in yeast mutants and methods of use thereof
KR101105718B1 (ko) 2005-10-27 2012-01-17 한국생명공학연구원 돌리칠 포스페이트 만노스 의존알파-1,3-만노실트랜스퍼라제를 코딩하는 한세눌라폴리모르파 기원의 신규 유전자와 상기 유전자가 결손된한세눌라 폴리모르파 변이주를 이용한 재조합 당단백질생산 방법
FR2912154B1 (fr) * 2007-02-02 2012-11-02 Glycode Levures genetiquement modifiees pour la production de glycoproteines homogenes
KR101811057B1 (ko) * 2007-04-03 2017-12-20 옥시레인 유케이 리미티드 분자의 글리코실화
WO2010138184A2 (en) * 2009-05-27 2010-12-02 Synageva Biopharma Corp. Avian derived antibodies
CN102712914A (zh) 2009-09-29 2012-10-03 根特大学 水解甘露糖-1-磷酸-6-甘露糖连接为磷酸-6-甘露糖
WO2011053545A1 (en) * 2009-10-30 2011-05-05 Merck Sharp & Dohme Corp. Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris
CA2777487A1 (en) * 2009-10-30 2011-05-05 Merck Sharp & Dohme Corp. Methods for the production of recombinant proteins with improved secretion efficiencies
BR112012011980A2 (pt) * 2009-11-19 2021-09-08 Oxyrane Uk Limited Métodos de produzir células transformadas de yarrowia lipolytica e proteínas-alvocompreendendo n-glicanos, células transformadas de yarrowia lipolytica e suas culturas, bem como composição compreendendo glicoproteínas
SG189108A1 (en) 2010-09-29 2013-05-31 Oxyrane Uk Ltd Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
EP2622089B1 (en) 2010-09-29 2023-11-01 Oxyrane UK Limited De-mannosylation of phosphorylated n-glycans
WO2012170292A1 (en) * 2011-06-07 2012-12-13 Merck Sharp & Dohme Corp. Modulation of metabolic pathways for improving bioprocess performance and secreted protein productivity of yeast
WO2013025527A1 (en) 2011-08-12 2013-02-21 Life Technologies Corporation Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis
KR20190114003A (ko) 2012-03-15 2019-10-08 옥시레인 유케이 리미티드 폼페병의 치료를 위한 방법 및 물질
WO2014093240A1 (en) 2012-12-10 2014-06-19 The General Hospital Corporation Bivalent il-2 fusion toxins
WO2014138188A1 (en) 2013-03-07 2014-09-12 The General Hospital Corporation Human ctla4 mutants and use thereof
WO2015100058A1 (en) * 2013-12-23 2015-07-02 Research Corporation Technologies, Inc. Pichia pastoris surface display system
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
AU2019325329A1 (en) * 2018-08-21 2021-04-08 Clara Foods Co. Modification of protein glycosylation in microorganisms
CA3130763A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
CN114375304A (zh) 2019-07-11 2022-04-19 克莱拉食品公司 蛋白质组合物及其食用品
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
US11639515B2 (en) * 2021-05-21 2023-05-02 Taixing Dongsheng Bio-Tech Co., Ltd Genetically engineered strain for producing porcine myoglobin and food-grade fermentation and purification thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US137134A (en) * 1873-03-25 Improvement in locomotive spark-arresters
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4837148A (en) 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4882279A (en) 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
US5080522A (en) 1988-12-13 1992-01-14 Shafirkin David I Device for locking and sealing of objects
AU8941191A (en) 1990-11-30 1992-06-25 Hsc Research And Development Limited Partnership Cloning of udp-n-acetylglucosamine:alpha-3-d-mannoside beta -1,2-n-acetylglucosaminyltransferase i
US5358967A (en) 1993-07-06 1994-10-25 Rohm And Haas Company Wool protecting compositons and methods
US5834251A (en) 1994-12-30 1998-11-10 Alko Group Ltd. Methods of modifying carbohydrate moieties
AU784960B2 (en) 1999-08-19 2006-08-10 Kirin Pharma Kabushiki Kaisha Novel yeast variants and process for producing glycoprotein containing mammalian type sugar chain
WO2002000879A2 (en) 2000-06-28 2002-01-03 Glycofi, Inc. Methods for producing modified glycoproteins
EP2267135A3 (en) 2000-06-30 2011-09-14 Vib Vzw Protein glycosylation modification in pichia pastoris

Also Published As

Publication number Publication date
WO2004003194A2 (en) 2004-01-08
US20040018588A1 (en) 2004-01-29
KR101047167B1 (ko) 2011-07-07
US7252933B2 (en) 2007-08-07
KR20050054875A (ko) 2005-06-10
JP2006502705A (ja) 2006-01-26
CA2490268A1 (en) 2004-01-08
WO2004003194A3 (en) 2004-04-22
IL165717A0 (en) 2006-01-15
MXPA04012983A (es) 2005-05-16
IL165717A (en) 2011-06-30
US20080009037A1 (en) 2008-01-10
CA2490268C (en) 2012-06-05
US8883445B2 (en) 2014-11-11
AU2003238051A1 (en) 2004-01-19
AU2003238051B2 (en) 2008-03-13
EP1516048A2 (en) 2005-03-23
EP2302047A1 (en) 2011-03-30

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