NO128370B - - Google Patents
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- Publication number
- NO128370B NO128370B NO04540/68A NO454068A NO128370B NO 128370 B NO128370 B NO 128370B NO 04540/68 A NO04540/68 A NO 04540/68A NO 454068 A NO454068 A NO 454068A NO 128370 B NO128370 B NO 128370B
- Authority
- NO
- Norway
- Prior art keywords
- enzyme
- preparations
- enzymes
- weight
- percent
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 claims description 70
- 108090000790 Enzymes Proteins 0.000 claims description 70
- 229940088598 enzyme Drugs 0.000 claims description 70
- 238000002360 preparation method Methods 0.000 claims description 54
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 37
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 36
- 239000000203 mixture Substances 0.000 claims description 26
- 239000003381 stabilizer Substances 0.000 claims description 23
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 20
- 239000004365 Protease Substances 0.000 claims description 20
- 102000035195 Peptidases Human genes 0.000 claims description 18
- 108091005804 Peptidases Proteins 0.000 claims description 18
- 125000004432 carbon atom Chemical group C* 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000005406 washing Methods 0.000 claims description 14
- 125000000217 alkyl group Chemical group 0.000 claims description 6
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 6
- LDMRLRNXHLPZJN-UHFFFAOYSA-N 3-propoxypropan-1-ol Chemical compound CCCOCCCO LDMRLRNXHLPZJN-UHFFFAOYSA-N 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 229940025131 amylases Drugs 0.000 claims description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 2
- 125000003545 alkoxy group Chemical group 0.000 claims description 2
- 125000002947 alkylene group Chemical group 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 125000001033 ether group Chemical group 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 239000001257 hydrogen Substances 0.000 claims description 2
- 239000003599 detergent Substances 0.000 description 42
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 16
- 239000007864 aqueous solution Substances 0.000 description 14
- 238000003860 storage Methods 0.000 description 14
- 229940079919 digestives enzyme preparation Drugs 0.000 description 11
- 150000001875 compounds Chemical class 0.000 description 10
- -1 ethanol Chemical class 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 230000000087 stabilizing effect Effects 0.000 description 9
- 108010056079 Subtilisins Proteins 0.000 description 7
- 102000005158 Subtilisins Human genes 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 239000003760 tallow Substances 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 239000000654 additive Substances 0.000 description 6
- 239000007859 condensation product Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 235000019419 proteases Nutrition 0.000 description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
- 239000004382 Amylase Substances 0.000 description 4
- 108091005658 Basic proteases Proteins 0.000 description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 108090000526 Papain Proteins 0.000 description 4
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
- 150000001298 alcohols Chemical class 0.000 description 4
- 102000004139 alpha-Amylases Human genes 0.000 description 4
- 108090000637 alpha-Amylases Proteins 0.000 description 4
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 4
- 239000002324 mouth wash Substances 0.000 description 4
- 229940051866 mouthwash Drugs 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- 229940055729 papain Drugs 0.000 description 4
- 235000019834 papain Nutrition 0.000 description 4
- 239000000600 sorbitol Substances 0.000 description 4
- 239000000271 synthetic detergent Substances 0.000 description 4
- NTKBNCABAMQDIG-UHFFFAOYSA-N trimethylene glycol-monobutyl ether Natural products CCCCOCCCO NTKBNCABAMQDIG-UHFFFAOYSA-N 0.000 description 4
- JCIIKRHCWVHVFF-UHFFFAOYSA-N 1,2,4-thiadiazol-5-amine;hydrochloride Chemical group Cl.NC1=NC=NS1 JCIIKRHCWVHVFF-UHFFFAOYSA-N 0.000 description 3
- POAOYUHQDCAZBD-UHFFFAOYSA-N 2-butoxyethanol Chemical compound CCCCOCCO POAOYUHQDCAZBD-UHFFFAOYSA-N 0.000 description 3
- 108010004032 Bromelains Proteins 0.000 description 3
- 108090000317 Chymotrypsin Proteins 0.000 description 3
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 108090000787 Subtilisin Proteins 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 235000019835 bromelain Nutrition 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 229960002376 chymotrypsin Drugs 0.000 description 3
- 229940096386 coconut alcohol Drugs 0.000 description 3
- 230000009849 deactivation Effects 0.000 description 3
- 210000000214 mouth Anatomy 0.000 description 3
- 229940096826 phenylmercuric acetate Drugs 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 229960001322 trypsin Drugs 0.000 description 3
- SIDULKZCBGMXJL-UHFFFAOYSA-N 1-dimethylphosphoryldodecane Chemical compound CCCCCCCCCCCCP(C)(C)=O SIDULKZCBGMXJL-UHFFFAOYSA-N 0.000 description 2
- CJPDBKNETSCHCH-UHFFFAOYSA-N 1-methylsulfinyldodecane Chemical compound CCCCCCCCCCCCS(C)=O CJPDBKNETSCHCH-UHFFFAOYSA-N 0.000 description 2
- JRZJOMJEPLMPRA-UHFFFAOYSA-N 1-nonene Chemical compound CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 2
- XNWFRZJHXBZDAG-UHFFFAOYSA-N 2-METHOXYETHANOL Chemical compound COCCO XNWFRZJHXBZDAG-UHFFFAOYSA-N 0.000 description 2
- WGKZYJXRTIPTCV-UHFFFAOYSA-N 2-butoxypropan-1-ol Chemical compound CCCCOC(C)CO WGKZYJXRTIPTCV-UHFFFAOYSA-N 0.000 description 2
- FDIPWBUDOCPIMH-UHFFFAOYSA-N 2-decylphenol Chemical compound CCCCCCCCCCC1=CC=CC=C1O FDIPWBUDOCPIMH-UHFFFAOYSA-N 0.000 description 2
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 description 2
- 229940093475 2-ethoxyethanol Drugs 0.000 description 2
- DEDUBNVYPMOFDR-UHFFFAOYSA-N 2-ethoxypropan-1-ol Chemical compound CCOC(C)CO DEDUBNVYPMOFDR-UHFFFAOYSA-N 0.000 description 2
- YTTFFPATQICAQN-UHFFFAOYSA-N 2-methoxypropan-1-ol Chemical compound COC(C)CO YTTFFPATQICAQN-UHFFFAOYSA-N 0.000 description 2
- YEYKMVJDLWJFOA-UHFFFAOYSA-N 2-propoxyethanol Chemical compound CCCOCCO YEYKMVJDLWJFOA-UHFFFAOYSA-N 0.000 description 2
- PSKIVCBTSGNKBB-UHFFFAOYSA-N 2-propoxypropan-1-ol Chemical compound CCCOC(C)CO PSKIVCBTSGNKBB-UHFFFAOYSA-N 0.000 description 2
- JDFDHBSESGTDAL-UHFFFAOYSA-N 3-methoxypropan-1-ol Chemical compound COCCCO JDFDHBSESGTDAL-UHFFFAOYSA-N 0.000 description 2
- 108091005508 Acid proteases Proteins 0.000 description 2
- 102000004400 Aminopeptidases Human genes 0.000 description 2
- 108090000915 Aminopeptidases Proteins 0.000 description 2
- 108010027805 Azocoll Proteins 0.000 description 2
- 102000005367 Carboxypeptidases Human genes 0.000 description 2
- 108010006303 Carboxypeptidases Proteins 0.000 description 2
- 244000060011 Cocos nucifera Species 0.000 description 2
- 235000013162 Cocos nucifera Nutrition 0.000 description 2
- 108060005980 Collagenase Proteins 0.000 description 2
- 102000029816 Collagenase Human genes 0.000 description 2
- 208000002064 Dental Plaque Diseases 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical group OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108091005507 Neutral proteases Proteins 0.000 description 2
- 108010067372 Pancreatic elastase Proteins 0.000 description 2
- 102000016387 Pancreatic elastase Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108010059712 Pronase Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 125000002252 acyl group Chemical group 0.000 description 2
- 108090000987 aspergillopepsin I Proteins 0.000 description 2
- CRHLZRRTZDFDAJ-UHFFFAOYSA-N butoxymethanol Chemical compound CCCCOCO CRHLZRRTZDFDAJ-UHFFFAOYSA-N 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 229940028356 diethylene glycol monobutyl ether Drugs 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- SYELZBGXAIXKHU-UHFFFAOYSA-N dodecyldimethylamine N-oxide Chemical compound CCCCCCCCCCCC[N+](C)(C)[O-] SYELZBGXAIXKHU-UHFFFAOYSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 108010059345 keratinase Proteins 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- VHWYCFISAQVCCP-UHFFFAOYSA-N methoxymethanol Chemical compound COCO VHWYCFISAQVCCP-UHFFFAOYSA-N 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 108090000021 oryzin Proteins 0.000 description 2
- MPQXHAGKBWFSNV-UHFFFAOYSA-N oxidophosphanium Chemical group [PH3]=O MPQXHAGKBWFSNV-UHFFFAOYSA-N 0.000 description 2
- JCGNDDUYTRNOFT-UHFFFAOYSA-N oxolane-2,4-dione Chemical compound O=C1COC(=O)C1 JCGNDDUYTRNOFT-UHFFFAOYSA-N 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- BPDIHEGWJJPPSG-UHFFFAOYSA-N propoxymethanol Chemical compound CCCOCO BPDIHEGWJJPPSG-UHFFFAOYSA-N 0.000 description 2
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- UCTWMZQNUQWSLP-VIFPVBQESA-N (R)-adrenaline Chemical compound CNC[C@H](O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-VIFPVBQESA-N 0.000 description 1
- HYTOZULGKGUFII-UHFFFAOYSA-N 1-methylsulfinyltridecan-3-ol Chemical compound CCCCCCCCCCC(O)CCS(C)=O HYTOZULGKGUFII-UHFFFAOYSA-N 0.000 description 1
- KWKAKUADMBZCLK-UHFFFAOYSA-N 1-octene Chemical compound CCCCCCC=C KWKAKUADMBZCLK-UHFFFAOYSA-N 0.000 description 1
- CGEGSCDKJJXMSB-UHFFFAOYSA-N 2-[dodecyl(2-hydroxyethyl)phosphoryl]ethanol Chemical compound CCCCCCCCCCCCP(=O)(CCO)CCO CGEGSCDKJJXMSB-UHFFFAOYSA-N 0.000 description 1
- TUBRCQBRKJXJEA-UHFFFAOYSA-N 3-[hexadecyl(dimethyl)azaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O TUBRCQBRKJXJEA-UHFFFAOYSA-N 0.000 description 1
- XHMWPVBQGARKQM-UHFFFAOYSA-N 3-ethoxy-1-propanol Chemical compound CCOCCCO XHMWPVBQGARKQM-UHFFFAOYSA-N 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- VCCWZAQTNBYODU-UHFFFAOYSA-N CC(=C)CC(C)CCC(C)=C Chemical group CC(=C)CC(C)CCC(C)=C VCCWZAQTNBYODU-UHFFFAOYSA-N 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical class S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical group C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 102000035092 Neutral proteases Human genes 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 108010067035 Pancrelipase Proteins 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000003342 alkenyl group Chemical group 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000003212 astringent agent Substances 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- RRLWYLINGKISHN-UHFFFAOYSA-N ethoxymethanol Chemical compound CCOCO RRLWYLINGKISHN-UHFFFAOYSA-N 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- HSEMFIZWXHQJAE-UHFFFAOYSA-N hexadecanamide Chemical compound CCCCCCCCCCCCCCCC(N)=O HSEMFIZWXHQJAE-UHFFFAOYSA-N 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- DZJFABDVWIPEIM-UHFFFAOYSA-N n,n-bis(2-hydroxyethyl)dodecan-1-amine oxide Chemical compound CCCCCCCCCCCC[N+]([O-])(CCO)CCO DZJFABDVWIPEIM-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- XYFCBTPGUUZFHI-UHFFFAOYSA-O phosphonium Chemical compound [PH4+] XYFCBTPGUUZFHI-UHFFFAOYSA-O 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- KCXFHTAICRTXLI-UHFFFAOYSA-M propane-1-sulfonate Chemical compound CCCS([O-])(=O)=O KCXFHTAICRTXLI-UHFFFAOYSA-M 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000003462 sulfoxides Chemical class 0.000 description 1
- 150000003512 tertiary amines Chemical class 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38663—Stabilised liquid enzyme compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S516/00—Colloid systems and wetting agents; subcombinations thereof; processes of
- Y10S516/01—Wetting, emulsifying, dispersing, or stabilizing agents
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Description
Foreliggende oppfinnelse angår stabiliserte vandige enzymholdige preparater egnet som rense- og vaskemidler og som omfatter: The present invention relates to stabilized aqueous enzyme-containing preparations suitable as cleaning and washing agents and which include:
1 ) fra 65 til 97 vektprosent vann,. 1 ) from 65 to 97 weight percent water,.
2) fra 0,001' til 1 ,0 vektprosent av et enzym valgt fra gruppen bestående av proteaser, oc-amylaser og blandinger derav, og 3) eventuelt fra..O til 1 5 vektprosent av , et vaske aktivt middel valgt fra gruppen beståend-e av , ikke-ioni s ke og zwitterioniske vaskeaktive midler, og 2) from 0.001' to 1.0 weight percent of an enzyme selected from the group consisting of proteases, oc-amylases and mixtures thereof, and 3) optionally from..0 to 1.5 weight percent of , a washing active agent selected from the group consisting of e of , non-ionic and zwitterionic detergents, and
h) fra 2 til 27 vektprosent av et stabiliserende middel, h) from 2 to 27 percent by weight of a stabilizing agent,
og det særegne ved preparatene i henhold til oppfinnelsen er al and the peculiarity of the preparations according to the invention is al
stabiliseringsmidlet er monphydroksyalkoholer med den generelle formel"■.<,-• the stabilizing agent is monohydroxyalcohols of the general formula"■.<,-•
hvori R^ er hydrogen eller en alkoksygruppe med formelen R-0-, hvor R ér en alkylgruppe med 1-8 karbonatomer og som kan inneholde 0 eller 1 etergruppe, og R2er en alkylengruppe med '1-U karbon- .: atomer. in which R 1 is hydrogen or an alkoxy group of the formula R-0-, where R is an alkyl group with 1-8 carbon atoms and which may contain 0 or 1 ether group, and R 2 is an alkylene group with 1-1 carbon atoms.
Andre trekk ved preparatene i henhold til oppfinnelsen fremgår Other features of the preparations according to the invention appear
av patentkravene. of the patent claims.
De vandige losninger av enzymer stabiliseres altså med tilsetning av den angitte type monohydroksyalkoholer. Eventuell ytterligere tilsetning av et ikke-ionisk eller zwitterionisk vaskeaktivt middel forbedrer stabiliseringen og gir onskelige overfiateaktive egenskaper til preparatene. The aqueous solutions of enzymes are thus stabilized with the addition of the indicated type of monohydroxy alcohols. Any further addition of a non-ionic or zwitterionic detergent improves stabilization and gives undesirable overactive properties to the preparations.
Enzymer selges vanlig i torr pulverisert form. De pulveriserte enzympreparater må beskyttes mot hoye temperaturer og hoye relative fuktigheter da enzymene ellers hurtig vil nedbrytes og/eller deaktiveres. Om dette se f.eks. "Alcalase", en industriell publika-sjon som utgis av Novo Industri A/S, Kobenhavn, Danmark.' Vandige enzympreparater omsettes vanligvis ikke på grunn av at enzymene nedbrytes og/eller deaktiveres hurtig i'vandige omgivelser. Enzymes are usually sold in dry powdered form. The powdered enzyme preparations must be protected against high temperatures and high relative humidity, otherwise the enzymes will quickly break down and/or deactivate. About this see e.g. "Alcalase", an industrial publication published by Novo Industri A/S, Copenhagen, Denmark.' Aqueous enzyme preparations are usually not converted because the enzymes are broken down and/or deactivated quickly in an aqueous environment.
Det er gjort forsok på å stabilisere enzymer i vandige losninger, se f.eks. US-patentskrift 3-296.09<*>+ hvor delvis hydrolysert og opploseliggjort.kollagen og glycerol anvendes for å stabilisere vandige losninger av proteolytiske enzymer. Denne fremgangsmåte for å stabilisere enzymene krever imidlertid store mengder glycerol, f eks. 35 til 60 vektprosent av den totale losning, og .. oker derfor prisen på enzymlosningen betraktelig. Attempts have been made to stabilize enzymes in aqueous solutions, see e.g. US patent 3-296.09<*>+ where partially hydrolyzed and solubilized collagen and glycerol are used to stabilize aqueous solutions of proteolytic enzymes. However, this method of stabilizing the enzymes requires large amounts of glycerol, e.g. 35 to 60 percent by weight of the total release, and therefore increases the price of the enzyme release considerably.
1 US-paténtskrift 3.095-358 foreslås sorbitol- anvendt for å stabilisere-vandige losninger inneholdende enzymer som f.eks. papain og blandinger av protease ogamylase oppnådd fraBacillus Subtilis. Denne metode, krever også store mengder (65 til 90 vektprosent av én80% sorbitollosning) av det stabiliserende middel sorbitol. Det store volum sorbitol som kreves for å stabilisere enzymene i vandige losninger virker som fortynningsmiddel og oker prisen på preparatet betraktelig. 1 US patent 3,095-358 suggests sorbitol - used to stabilize - aqueous solutions containing enzymes such as e.g. papain and mixtures of protease and amylase obtained from Bacillus Subtilis. This method also requires large amounts (65 to 90 percent by weight of an 80% sorbitol solution) of the stabilizing agent sorbitol. The large volume of sorbitol required to stabilize the enzymes in aqueous solutions acts as a diluent and increases the price of the preparation considerably.
Disse to tidligere forsok er illustrerende for de vesentlige problemer som man stoter på når man forsoker å stabilisere vandige losninger av enzymer: (1) utgifter.. (2) stor fortynning av det vandige produkt med et stabiliserende middel. These two previous attempts are illustrative of the significant problems encountered when attempting to stabilize aqueous solutions of enzymes: (1) expense... (2) large dilution of the aqueous product with a stabilizing agent.
De stabiliserte vandige enzymholdige preparater i henhold til foreliggende oppfinnelse eliminerer de problemer som var forbundet med den tidligere kjente teknikk. Mindre enn 30% åv enzympreparatet utgjores av stabiliserende middel, og de stabiliserende midler 'som anvendes er lett tilgjengelige til rimelig pris. De enzymstabiliserende forbindelser som anvendes ved den foreliggende oppfinnelse besitter nyttige losningsmiddelegenskaper og er derfor onskelige når preparatet anvendes.for detergentformål. Alkoholene, f.eks. etanol, er også utmerkede astringerende midler og tjener således en verdifull funksjon når preparatet anvendes som munnvann. Tilsetning av de.ytterligere ikke-ioniske eller zwitterioniske vaskeaktive komponenter forbedrer stabiliteten av enzymene i det vandige preparat, og. forbedrer de vaskeaktive egenskapene for dette. The stabilized aqueous enzyme-containing preparations according to the present invention eliminate the problems associated with the previously known technique. Less than 30% of the enzyme preparation is made up of stabilizing agents, and the stabilizing agents used are readily available at a reasonable price. The enzyme stabilizing compounds used in the present invention possess useful solvent properties and are therefore undesirable when the preparation is used for detergent purposes. The alcohols, e.g. ethanol, are also excellent astringents and thus serve a valuable function when the preparation is used as a mouthwash. Addition of the additional non-ionic or zwitterionic detergent active components improves the stability of the enzymes in the aqueous preparation, and. improves the detergent-active properties for this.
Fig. 1 illustrerer den stabiliserende virkning av forskjellige konsentrasjoner av de stabiliseringsmidler som anvendes i en vandig losning innfeholdende 1 % "Alcalase"■ ( 6% krystallinsk enzym) og % étoksylert talgalkohol (1 mol talgalkohol etoksylert med 30 mol etylenoksyd) holdt ved en pH på 7,0. Det fremgår klart at-'-enzymstabiliteten kan optimaliseres ved å anvende angjeldende stabiliseringsmidler i de foretrukne effektive mengder. Det er også-klart-at de foretrukne stabiliseringsmidler, d.v.s. metanol; etanol og isopropanol, kan anvendes for maksimalisering av enzym-stabiliteten over lange lagringsperioder. Fig. 1 illustrates the stabilizing effect of different concentrations of the stabilizing agents used in an aqueous solution containing 1% "Alcalase" (6% crystalline enzyme) and % ethoxylated tallow alcohol (1 mol of tallow alcohol ethoxylated with 30 mol of ethylene oxide) maintained at a pH of 7.0. It is clear that enzyme stability can be optimized by using relevant stabilizers in the preferred effective amounts. It is also clear that the preferred stabilizers, i.e. methanol; ethanol and isopropanol, can be used to maximize enzyme stability over long storage periods.
Granulerte vaskemiddelblandinger inneholdende enzymer er anvendt Granulated detergent mixtures containing enzymes have been used
i Europa i adskillige år og er nylig tatt i bruk også i andre land. Disse enzymholdige granulerte vaskemiddelblandinger er.særlig effektive for å fjerne flekker fra tekstiler, husholdingsgjenstander in Europe for several years and has recently been adopted in other countries as well. These enzyme-containing granulated detergent mixtures are particularly effective for removing stains from textiles, household items
gulver og vegger. Disse granuDarte - vaskemiddelblandinger er imidlertid: brysomme å-,anvende..i ,små renseoperasjoner.som f.eks.-:-flekkf jernihgi'..'■ floors and walls. These granular detergent mixtures are, however, difficult to use in small cleaning operations such as stain removal.
Denne mangel overvinnes imidlertid med de foreliggende preparater, idet de-vandige enzymholdige ; preparater, er greie å .-anvende som However, this deficiency is overcome with the present preparations, as they contain aqueous enzymes; preparations, are fine to .-use as
flekkfjerrier, vaskemiddelådditiv,^vaskemiddel eller, munnvann. I-tillegg er enzympreparatene stabile over lange lagringsperioder. stain remover, detergent additive, detergent or mouthwash. In addition, the enzyme preparations are stable over long storage periods.
Preparatene omfatter tre vesentlige komponenter, nemlig vann, enzymer og, stabiliserende-midler. Ikke-ioniske eller zwitter^ioniske vaskeaktive..midler kan tilsettes^preparatene som eventuelle bestanddeler. Disse komponenter og de mengder hvori' de anvendes heri er droftet i det folgende. The preparations include three essential components, namely water, enzymes and stabilizers. Non-ionic or zwitterionic detergents can be added to the preparations as possible components. These components and the quantities in which they are used herein are set out below.
Vann omfatter hovedandelen av preparatene og anvendes generelt i mengder som utgjor fra 65 til. 97 vektprosent av-preparatene og fortrinnsvis fra 72 til 95 vektprosent. Avionisert vann foretrekkes, selv om dette ikke er uomgjengelig nodvendig. Water comprises the main part of the preparations and is generally used in amounts that amount to from 65 to. 97 percent by weight of the preparations and preferably from 72 to 95 percent by weight. Deionized water is preferred, although this is not absolutely necessary.
Enzymer som er egnet for denne anvendelse og som stabiliseres Enzymes which are suitable for this application and which are stabilized
i vandig losning omfatter de alkaliske, noytrale og sure proteaser'og a-amylaser. Proteasene i henhold til denne klassifisering in aqueous solution they include alkaline, neutral and acid proteases and α-amylases. The proteases according to this classification
utvinnes vanlig fra kilder som sopp og bakterier. Enzymer utvunnet fra kilder som planter og dyr kan også.anvendes heriy men klassi-fiseres ikke så greit i de nevnte alkaliske, noytrale og-sure underklasser. Disse enzymer er aktive i pB området fra omtrent 3 til omtrent 11 og ved temperaturer fra omtrent h til omtrent 77°C. Optimal aktivitet av disse proteaser utvises generelt i pH området fra omtrent 5,0 til 10,0 og fortrinnsvis fra 6,0 til 9,5. is commonly extracted from sources such as fungi and bacteria. Enzymes extracted from sources such as plants and animals can also be used here but are not so neatly classified into the aforementioned alkaline, neutral and acidic subclasses. These enzymes are active in the pB range from about 3 to about 11 and at temperatures from about h to about 77°C. Optimal activity of these proteases is generally exhibited in the pH range from approximately 5.0 to 10.0 and preferably from 6.0 to 9.5.
Proteasene er særlig effektive for nedbryting av proteinsmuss. Proteasene katalyserer hydrolysen av peptidbindingene i proteiner, polypeptider og beslektede forbindelser. Det oppnås derved frie aminogrupper og karboksylgrupper og de langkjedete proteinstruk-turer reduseres til. flere kortere kjeder. Disse kortere kjeder kan'lett fjernes fra deres omgivelser med vånn eller vandige vaskemiddelblandinger.. The proteases are particularly effective for breaking down protein dirt. The proteases catalyze the hydrolysis of the peptide bonds in proteins, polypeptides and related compounds. Free amino groups and carboxyl groups are thereby obtained and the long-chain protein structures are reduced to several shorter chains. These shorter chains can be easily removed from their surroundings with water or aqueous detergent mixtures.
De alkaliske proteaser er de enzymer som spesielt foretrekkes. Alkaliske.proteaser som foretrekkes omfatter subtilisin, "BPN", elastase, keratinase, karboksypeptidase, aminopeptidase., aspergillopeptidase A og' aspergillopeptidase B. Subtilisin og "BPN" foretrekkes særlig.. De alkaliske. proteaser foretrekkes særlig da de j fremviser optimal aktivitet i pH-området for normale vaskemiddel- , komponenter, d.v.s. fra 7>5til 10,5,- og de alkaliske proteaser fremviser overraskende;stabilitet i preparatene. The alkaline proteases are the enzymes that are particularly preferred. Preferred alkaline proteases include subtilisin, "BPN", elastase, keratinase, carboxypeptidase, aminopeptidase, aspergillopeptidase A and aspergillopeptidase B. Subtilisin and "BPN" are particularly preferred. The alkaline. proteases are particularly preferred as they exhibit optimal activity in the pH range for normal detergent components, i.e. from 7>5 to 10.5, and the alkaline proteases surprisingly exhibit stability in the preparations.
De noytrale proteaser som også kan anvendes i preparatene omfatter kollagenase, chymotrypsin og trypsin og de proteolytiske enzymer som isoleres fra streptomyces-artene. Både chymotrypsin og trypsin fremviser optimal aktivitet i det noytrale til alkaliske området. The neutral proteases that can also be used in the preparations include collagenase, chymotrypsin and trypsin and the proteolytic enzymes that are isolated from the streptomyces species. Both chymotrypsin and trypsin exhibit optimal activity in the neutral to alkaline range.
Eksempler på sure proteaser som egner seg omfatter pepsin, papain Examples of suitable acid proteases include pepsin, papain
og bromelin. Både papain og bromelin fremviser optimal aktivitet i det sure til noytrale- pH-område. and bromelain. Both papain and bromelain exhibit optimal activity in the acidic to neutral pH range.
Videre kan a-amylasene også stabiliseres i preparatene. Samtlige oc-amylaser fremviser optimal aktivitet i det sure'område, Furthermore, the α-amylases can also be stabilized in the preparations. All oc-amylases exhibit optimal activity in the acidic range,
a-amylasene er særlig'godt egnet for nedbryting av stivelses-' molekyler da de angriper a-^^-glykosidbindingene i stivelse. De gjenværende kortere kjeder fjernes lett fra deres omgivelser med vann eller vandige losninger av vaskemidler, a-amylasene kan oppnås fra animalske kilder, korn, og fra kilder som bakterier eller sopp. The α-amylases are particularly suitable for breaking down starch molecules as they attack the α-^^-glycosidic bonds in starch. The remaining shorter chains are easily removed from their surroundings with water or aqueous solutions of detergents, the α-amylases can be obtained from animal sources, grains, and from sources such as bacteria or fungi.
Kommersielle enzymblandinger inneholdende de nevnte enzymer kan anvendes. Disse kommersielle enzymblandinger selges vanlig i torr, pulverisert form bestående av fra omtrent 2 til omtrent 80% aktive enzymer i kombinasjon med en inert pulverisert bærer som f.eks. natrium- eller kalsium-sulfat eller natriumklorid som de ræterende 20 til . 98%. Det aktive enzyminnhold i kommersielle enzymblandinger er resultatet av.de anvendte fremstillingsmetoder og er ikke kritisk så lenge de ferdige preparater.oppnår di angitte enzyminnhold. De uloselige inerte materialer fjernes vanlig fra preparatene for å gi disse en god klarhet uten bunnfall. Commercial enzyme mixtures containing the aforementioned enzymes can be used. These commercial enzyme mixtures are commonly sold in dry, powdered form consisting of from about 2 to about 80% active enzymes in combination with an inert powdered carrier such as sodium or calcium sulfate or sodium chloride such as the irrigating 20 to . 98%. The active enzyme content in commercial enzyme mixtures is the result of the production methods used and is not critical as long as the finished preparations achieve the specified enzyme content. The insoluble inert materials are usually removed from the preparations to give them a good clarity without sediment.
Store variasjbher av mengden enzymer i de handelsvanlige blandinger kan forekomme, og de. blandinger som vanlig kan fås i handelen kan inneholde fra omtrent 0,001. til omtrent 1 vektprosent enzymer. For'de beste resultater inneholder blandingene fortrinnsvis fra"0,01 til 0,5 .vektprosent enzymer. Når en av de foretrukne enzymblandinger anvendes heri,■inneholder det ferdige preparat fortrinnsvis fra 0,1 til omtrent ^.vektprosent av enzymblandingen som solgt i kommersiell form., f.eks. inneholdende fra 2 til 80% aktive enzymer. Det aktive enzyminnhold i de vandige enzympreparater i . henhold til oppfinnelsen bor .dog utgjore fra 0,001 til 1 vektprosent som skissert i det foregående. Large variations in the amount of enzymes in the commercial mixtures can occur, and they. commercially available mixtures may contain from about 0.001. to about 1% by weight of enzymes. For best results, the compositions preferably contain from 0.01 to 0.5 weight percent of enzymes. When one of the preferred enzyme compositions is used herein, the finished preparation preferably contains from 0.1 to about 2 weight percent of the enzyme mixture as sold in commercial form, for example containing from 2 to 80% active enzymes. The active enzyme content in the aqueous enzyme preparations according to the invention should, however, be from 0.001 to 1 percent by weight as outlined above.
De stabiliserende midler som stabiliserer de enzymer som er beskrevet ovenfor velges som nevnt fra monohydroksyalkoholer av den angitte type. The stabilizing agents which stabilize the enzymes described above are chosen as mentioned from monohydroxyalcohols of the indicated type.
Spesifikke 'eksempler på passende monohydroksyalkoholer omfatter metanol, etanol, propanol, isopropanol, butanol og isobutanol. Eksempler på alkoksymonohydroksyalkoholer omfatter metoksymetanol, 2-metoksy-e tanol, 3-metoksypropanol, 2-metoks ypropanol, etoksy-metanol, 2-etoksyetanol, 3-etoksypropanol, 2-etoksypropanol, propoksymetanol, 2-propoksyetanol, 3-propoksypropanol, 2-propoksy-propanol, butoksymetanol, 2-butoksyetanol, 3-butoksypropanol, og 2-butoksypropanol. Specific examples of suitable monohydroxy alcohols include methanol, ethanol, propanol, isopropanol, butanol and isobutanol. Examples of alkoxymonohydroxyalcohols include methoxymethanol, 2-methoxyethanol, 3-methoxypropanol, 2-methoxypropanol, ethoxymethanol, 2-ethoxyethanol, 3-ethoxypropanol, 2-ethoxypropanol, propoxymethanol, 2-propoxyethanol, 3-propoxypropanol, 2- propoxypropanol, butoxymethanol, 2-butoxyethanol, 3-butoxypropanol, and 2-butoxypropanol.
Foretrukne stabiliseringsmidler for anvendelse heri er metanol, etanol, isopropanol, propanol, 3-propoksypropanol samt dietylen-glykol-monobutyleter. Metanol, etanol og isopropanol foretrekkes særlig. Metanol og stabiliseringsmidler avledet fra metanol, bor ikke anvendes i vandige enzympreparater for innvortes bruk, på grunn av deres giftighet. Preferred stabilizers for use herein are methanol, ethanol, isopropanol, propanol, 3-propoxypropanol and diethylene glycol monobutyl ether. Methanol, ethanol and isopropanol are particularly preferred. Methanol and stabilizers derived from methanol should not be used in aqueous enzyme preparations for internal use, due to their toxicity.
De enzymstabiliserende forbindelser som er beskrevet ovenfor kan, som nevnt, anvendes i preparatene i effektive mengder som utgjor fra 2 til 27 vektprosent av preparatene. For imidlertid å oppnå optimale stabiliserende virkninger for lengre lagringsperioder ved hoye temperaturer, bor stabiliseringsmidlene anvendes i de brukskonsentrasjoner som er angitt i den folgende tabell I som skisserer de mengder av metanol, etanol og isopropanol som må være tilstede' i disse vandige enzympreparater for å bibeholde omtrent 50% enzymaktivitet etter lagring ved 37j8°C i 5 uker. The enzyme-stabilizing compounds described above can, as mentioned, be used in the preparations in effective amounts which constitute from 2 to 27 percent by weight of the preparations. However, in order to achieve optimum stabilizing effects for longer storage periods at high temperatures, the stabilizing agents should be used in the usage concentrations indicated in the following table I which outlines the amounts of methanol, ethanol and isopropanol that must be present' in these aqueous enzyme preparations in order to maintain approximately 50% enzyme activity after storage at 37j8°C for 5 weeks.
Vannloselige ikke-ioniske og zwitterioniske vaskeaktive midler kan anvendes som eventuelle bestanddeler i preparatene. Disse vaskeaktive midler forbedrer lagringsstabiliteten av de anvendte enzymer og forbedrer bemerkelsesverdig vaske-egenskapene for preparatene. På grunn av disse nyttige egenskaper foretrekkes det å inkludere ikke-ioniske og zwitterioniske vaskeaktive midler i de vandige enzympreparater, særlig den som inneholder oc-amylase. De ikke-ioniske og zwltter-ioniske vaskeaktive midler kan anvendes heri i mengder som utgjor fra 0 til 15 vektprosent, fortrinnsvis fra h til 10 vektprosent av preparatene. Water-soluble non-ionic and zwitterionic detergents can be used as possible components in the preparations. These detergent active agents improve the storage stability of the enzymes used and remarkably improve the washing properties of the preparations. Because of these useful properties, it is preferred to include nonionic and zwitterionic detergents in the aqueous enzyme preparations, especially those containing oc-amylase. The non-ionic and zwlter-ionic detergents can be used here in amounts that make up from 0 to 15 percent by weight, preferably from 1 to 10 percent by weight of the preparations.
Når det anvendes oc-amylase,. foretrekkes det å anvende omtrent 2 til 15 vektprosent av det ikke-ioniske eller zwitterioniske vaskeaktive middel; When oc-amylase is used, it is preferred to use about 2 to 15 percent by weight of the nonionic or zwitterionic detergent;
Eksempler på passende ikke-ioniske vaskeaktive midler for anvendelse heri omfatter: Examples of suitable nonionic detergents for use herein include:
(1) Polyetylerioksydkondensatene av alkylfenoler, f.eks. konden-sasjons produktene av alkylfenoler med en alkylgruppé inneholdende fra 6 til 12 karbonatomer i enten rett eller f orgrenet kjede--konf iguras jon med etylenoksyd, idet'etylenoksydet er tilstede.i . mengder tilsvarende 5 til.25 mol etylenoksyd pr. moi alkylfenol. . Alkylsubstituenten i disse .forbindelser kan f.eks. avledes fra polymerisert propylen, diisobutylen, okten eller nonen.. (2) De ikke-ioniske syntetiske vaskeaktive midler som avledes fra kondenseringen av etylenoksyd med'det produkt som resulterer fra omsetningen mellom propylenoksyd og etylendiamin..Således.er forbindelser- inneholdende fra omtrent kO til omtrent 80 vektprosent polyoksyetylen og med en molekylvekt på fra. omtrent 5000 til omtrent11.000 og som skriver seg fra omsetningen mellom etylenoksyd-grupper og en hydrofob base bestående av reaksjonsproduktet av"etylendiamin og overskudd av propylenoksyd, idet basen har en molekylvekt på omtrent 2500 til 3000, tilfredsstillende. (3) Kondensasjonsproduktet av 1 mol alifatiske alkoholer med fra 8 til 22 karbonatomer, i enten rettkjedet eller forgrenet kjedekonfigurasjon, med fra 5 til h0 mol etylenoksyd, f.eks. et kokosnott-alkoholetylenoksydkondensat med fra 5 til ^0 mol etylenoksyd pr. mol kokosnottalkohol, idet kokosnottalkoholfraksjonen har fra 10 til 1<>>+ karbonatomer. (!+) De usubstituerte amider_ og monoetanol- og dietanol-amider av fettsyrer med acyldeler med fra omtrent 8 til omtrent 22 karbonatomer. Disse acyldeler avledes vanlig fra naturiig forekommende glycerider, som f.eks. kokosnottolje, palmeolje, soyabonneolje, og talg, men kan fremstilles syntetisk, f.eks. ved oksydasjon av petroleum eller ved hydrogenering av karbonmonoksyd ved hjelp av Fischer-Tropsch prosessen. (5) Langkjedete tertiære aminoksyder tilsvarende den folgende generelle formel (1) The polyethylene oxide condensates of alkylphenols, e.g. the condensation products of alkylphenols with an alkyl group containing from 6 to 12 carbon atoms in either a straight or branched chain configuration with ethylene oxide, the ethylene oxide being present. amounts corresponding to 5 to 25 mol of ethylene oxide per moi alkylphenol. . The alkyl substituent in these compounds can e.g. derived from polymerized propylene, diisobutylene, octene or nonene.. (2) The nonionic synthetic detergents derived from the condensation of ethylene oxide with the product resulting from the reaction between propylene oxide and ethylenediamine..Thus.are compounds containing from about kO to about 80 percent by weight polyoxyethylene and with a molecular weight of from. approximately 5,000 to approximately 11,000 and which results from the reaction between ethylene oxide groups and a hydrophobic base consisting of the reaction product of "ethylenediamine and excess propylene oxide, the base having a molecular weight of approximately 2,500 to 3,000, satisfactory. (3) The condensation product of 1 moles of aliphatic alcohols of from 8 to 22 carbon atoms, in either straight chain or branched chain configuration, with from 5 to h0 moles of ethylene oxide, eg a coconut alcohol ethylene oxide condensate with from 5 to ^0 moles of ethylene oxide per mole of coconut alcohol, the coconut alcohol fraction having from 10 to 1<>>+ carbon atoms. (!+) The unsubstituted amides_ and monoethanol and diethanol amides of fatty acids having acyl moieties of from about 8 to about 22 carbon atoms. These acyl moieties are usually derived from naturally occurring glycerides, such as e.g. coconut oil, palm oil, soybean oil, and tallow, but can be produced synthetically, e.g. by oxidation of petroleum or by hydrogenation of carbon monoxide using the Fischer-Tropsch process. (5) Long-chain tertiary amine oxides corresponding to the following general formula
hvori R er et alkylradi-kal med fra omtrent 8 til omtrent 22 karbonatomer, R 2 og RJ 3 er hver metyl, etyl eller hydroksyetyl- 1; radikaler, R er etylen, og n er et tall fra 0 til omtrent 10. Pilen.i formelen er en vanlig angivelse, av en halvpolar binding. wherein R is an alkyl radical having from about 8 to about 22 carbon atoms, R 2 and RJ 3 are each methyl, ethyl or hydroxyethyl-1; radicals, R is ethylene, and n is a number from 0 to about 10. The arrow in the formula is a common indication of a semipolar bond.
Spesifikke eksempler på aminoksyd-vaskeaktive midler omfatter: dimetyl-dodecylaminoksyd og bis-(2-hydroksyetyl)-dodecylaminoksyd. (6) Langkjedete tertiære fosfinoksyder tilsvarende.den folgende geneielle formel RR'R"P-> 0 hvori R er et alkyl-, alkenyl- eller monohydroksyalkylradikal med fra 10 til 22 karbonatomer i kjede-lengden og R<1>og R" er hver alkyl- eller monohydroksyalkyl-grupper inneholdende fra 1 til 3 karbonatomer. Pilen i formelen er en vanlig representasjon av en halvpolar binding. Eksempler på passende f osf inoksyder finnes i US-patentskrif t 3»30<!>+.263 og omfatter dimetyl-dodecylfosfinoksyd og bis-(2-hydroksyetyl)dodecylfosfinoksyd. (7) Langkjedete sulfoksyder med den generelle formel Specific examples of amine oxide detergents include: dimethyl dodecyl amine oxide and bis-(2-hydroxyethyl) dodecyl amine oxide. (6) Long-chain tertiary phosphine oxides corresponding to the following general formula RR'R"P-> 0 in which R is an alkyl, alkenyl or monohydroxyalkyl radical with from 10 to 22 carbon atoms in the chain length and R<1> and R" are each alkyl or monohydroxyalkyl groups containing from 1 to 3 carbon atoms. The arrow in the formula is a common representation of a semipolar bond. Examples of suitable phosphine oxides are found in US Patent 3,30<!>+,263 and include dimethyl dodecyl phosphine oxide and bis-(2-hydroxyethyl)dodecyl phosphine oxide. (7) Long-chain sulfoxides of the general formula
■hvori R er et alkylradikal inneholdende fra omtrent 10 til omtrent 22 karbonatomer, fra 0 til omtrent 5 eterbindinger og fra 0 til omtrent 2 hydroksylsubstituenter, idet minst en del av Ry er et alkylradikal inneholdende 0 eterbindinp°og inneholdende fra omtrent 10 til omtrent 18 karbonatomer, og.hvori R 6 er et alkylradikal inneholdende fra 1 til 3 karbonatomer og fra 1 til 2 hydroksylgrupper. Spesifikke eksempler på disse sulfoksyder er: dodecylmetylsulfoksyd og 3-hydroksytridecylmetylsulfoksyd.• wherein R is an alkyl radical containing from about 10 to about 22 carbon atoms, from 0 to about 5 ether linkages and from 0 to about 2 hydroxyl substituents, at least a portion of Ry being an alkyl radical containing 0 ether linkages and containing from about 10 to about 18 carbon atoms, and in which R 6 is an alkyl radical containing from 1 to 3 carbon atoms and from 1 to 2 hydroxyl groups. Specific examples of these sulphoxides are: dodecylmethyl sulphoxide and 3-hydroxytridecylmethyl sulphoxide.•
De zwitterioniske syntetiske vaskeaktive midler som kan anvendes kan generelt beskrives som derivater av alifatiske kvartære ammonium- fosfonium- og sulfonium-forbindelser, hvori det alifatiske radikal kan være rettkjedet eller forgrenet, og hvori en ay de alifatiske substituenter inneholder fra omtrent 8 til omtrent 22 karbonatomer og en inneholder en anionisk vann-opploseliggjorende gruppe som f.eks. karboksy-, sulfo-i, f osf ato-eller f osf ono-grupper. EkseMpiér?på;; forbindelser som faller innenfor denne definisjon er 3-(N,N-dimetyl-N-heksadecylammonio)propan-1 -sulfonat og 3~(N,N-dimetyl-N-heksade.cylammonio)-2-hydroksypropan-1-sulf onatFor ytterligere.'eksempler' på zwitterioniske syntetiske vaskeaktive ■ midler, se. canadisk patentskrift 708. lh7, side 6 li-njer 1-22;. "Laundering .Fabrics • in. Cold Water.Containing a Synthetic Detergent Composition". The zwitterionic synthetic detergents that can be used can generally be described as derivatives of aliphatic quaternary ammonium, phosphonium and sulfonium compounds, in which the aliphatic radical can be straight chain or branched, and in which ay the aliphatic substituents contain from about 8 to about 22 carbon atoms and one contains an anionic water-solubilizing group such as carboxy, sulfo-i, phosphate or phosphate groups. ExeMpiér?on;; compounds that fall within this definition are 3-(N,N-dimethyl-N-hexadecylammonio)propane-1 -sulfonate and 3~(N,N-dimethyl-N-hexade.cylammonio)-2-hydroxypropane-1-sulfonateFor further.'examples' of zwitterionic synthetic detergents ■ see. Canadian patent document 708. lh7, page 6 lines 1-22;. "Laundering .Fabrics • in. Cold Water.Containing a Synthetic Detergent Composition".
Blandinger av forskjellige ikke-ioniske vaskeaktive.midler eller blandinger av ikke-ioniske vaskeaktive midler og zwitterioniske vaskeaktive midler kan med fordel anvendes... Mixtures of different non-ionic detergents or mixtures of non-ionic detergents and zwitterionic detergents can be advantageously used...
De forskjellige komponenter i enzympreparatene i henhold til oppfinnelsen kan blandes sammen ihvilken som helst rekkefolge.. Det foretrekkes imidlertid at-alkohol-vannblandingen fremstilles forst og enzymene tilsettes dertil for å forhindre eventuell nedbryting eller deaktivering i losninger som overveiende består av enten vann eller alkohol. Den eller de eventuelle vaskeaktive komponenter kan tilsettes når som helst. The various components of the enzyme preparations according to the invention can be mixed together in any order. However, it is preferred that the alcohol-water mixture is prepared first and the enzymes added to it to prevent possible breakdown or deactivation in solutions that predominantly consist of either water or alcohol. The detergent active component(s), if any, can be added at any time.
pH i de stabiliserte vandige enzympreparater er vanlig fra omtrent 5,0 til 10,0 og er fortrinnsvis fra omtrent 6,5 til omtrent 8,5. Maksimale stabiliserende påvirkninger oppnås i det foretrukne pH-ornråde. Videre kan pH okes med en base, f. eks. natrium- eller kaliumhydroksyd, eller senkes med en syre som f.eks. saltsyre. The pH of the stabilized aqueous enzyme preparations is usually from about 5.0 to about 10.0 and is preferably from about 6.5 to about 8.5. Maximum stabilizing effects are achieved in the preferred pH range. Furthermore, the pH can be increased with a base, e.g. sodium or potassium hydroxide, or lowered with an acid such as hydrochloric acid.
Det foretrekkes også, selv om dette ikke er obligatorisk, at et konserveringsmiddel tilsettes preparatene for å forhindre vekst av bakterier og sopp. Et særlig godt konserveringsmiddel er fenylmerkuriacetat som generelt anvendes i mengder på fra omtrent-10 til omtrent kO deler pr. million av preparatet..Hvilket som neist konserveringsmiddel som er blandbart med komponentene i preparatene kan anvendes. It is also preferred, although this is not mandatory, that a preservative is added to the preparations to prevent the growth of bacteria and fungi. A particularly good preservative is phenyl mercuric acetate, which is generally used in amounts of from about -10 to about k0 parts per million of the preparation..Which neist preservative that is miscible with the components in the preparations can be used.
Enzympreparatene inneholdende vann, stabiliseringsmidler , ■ enzymer The enzyme preparations containing water, stabilizers, ■ enzymes
og eventuelt ikke-ioniske og/eller zwitterioniske vaskeaktive midler kan anvendes som en flekkfjerner, vaskemiddeladditiv eller som en-vaskemiddelblanding. Preparatene kan pakkes i flasker av dusjetypen and possibly non-ionic and/or zwitterionic detergents can be used as a stain remover, detergent additive or as a detergent mixture. The preparations can be packaged in shower-type bottles
og greit anvendes for å fjerne relativt små flekker fra.tekstiler eller kan også anvendes i storre mengder som et vaskemiddel-, additiv. Preparatene kan anvendes i stedet for hypoklorit-bleke-midla? da de fjerner mange av de flekker som disse blekemidler fjerner, og som en ytterligere fordel vil de ikke angripe eller nedbryte fluorescerende bestanddeler og hvitemidler. Med tilsetning av de eventuelle ikke-ioniske og/eller zwitterioniske vaskeaktive midler kan preparatet anvendes som det eneste vaske-, middel i en vaske operas jon. and is easily used to remove relatively small stains from textiles or can also be used in large quantities as a detergent additive. Can the preparations be used instead of hypochlorite bleach? as they remove many of the stains that these bleaches remove, and as a further advantage they will not attack or degrade fluorescent components and whiteners. With the addition of any non-ionic and/or zwitterionic detergents, the preparation can be used as the only detergent in a washing operation.
Preparatme er også brukelige som munnvann. Det alkohol-holdige stabiliserende middel, fortrinnsvis etanol, virker som et astringerende middel mens enzymene er effektive for å fjerne dental-belegg, fjåsne matpartikler fra gingivalhulen og fjerne slimbelegg fra munnen. Preparations are also usable as a mouthwash. The alcohol-containing stabilizing agent, preferably ethanol, acts as an astringent while the enzymes are effective in removing dental plaque, trapping food particles from the gingival cavity and removing mucus plaque from the mouth.
Eksempler. Examples.
De folgende eksempler skal illustrere oppfinnelsen i detalj og The following examples shall illustrate the invention in detail and
når disse sees i forbindelse med den foregående beskrivelse, vil oppfinnelsen lettere forstås. Alle deler, prosentmengder og forhold som er angitt er basert på vekt med mindre annet er angitt. when these are seen in connection with the preceding description, the invention will be more easily understood. All parts, percentages and ratios given are by weight unless otherwise stated.
De folgende preparater (eksemplene 1-1.7) ble fremstilt og lagret The following preparations (Examples 1-1.7) were prepared and stored
i lukkede glassflasker i de angitte tidsrom. Eksemplene 1 , 2 og 2h er ikke eksempler på oppf inreLsen, mener bare tatt med for sammenligningsformål. in closed glass bottles during the specified periods. Examples 1, 2 and 2h are not examples of the invention, but only included for comparison purposes.
Fotnoter til eksemplene 1 til og med 27. Footnotes to examples 1 to 27 inclusive.
1. "IAE^q" - T mol-talgalkohol etoksylert -med 30 mol.etylenoksyd. 2. "Alcalase" - et. kommersielt proteolytisk enzympre.parat méd et krystallinsk .enzyminnhold på omtrent 6%.. 3. Prosent gjenværende aktivitet ble bestemt ved hjelp av "Azocoll"-metoden. Denne metode er basert på frigivelse av et vannloselig farvestoff fra e: vannuopploselig protein-farvestoff-substrat .("Azocoll") ved hjelp av et proteolytisk enzym. Den mengde farvstoff som frigis under noye kontrollerte betingelser måles spektrofotometrisk. Enzymatisk aktivitet beregnes fra den frigitte mengde farvestoff. Initial aktivitet tilsvarer 100%. 1. "IAE^q" - T mol tallow alcohol ethoxylated - with 30 mol ethylene oxide. 2. "Alcalase" - et. commercial proteolytic enzyme preparation with a crystalline enzyme content of approximately 6%. 3. Percent residual activity was determined by the "Azocoll" method. This method is based on the release of a water-insoluble dye from e: water-insoluble protein-dye-substrate .("Azocoll") by means of a proteolytic enzyme. The amount of dye that is released under carefully controlled conditions is measured spectrophotometrically. Enzymatic activity is calculated from the released amount of dye. Initial activity corresponds to 100%.
h. 30 deler pr. million fenylmerkuriacetat ble tilsatt hvert preparat for å bremse vekst av bakterier og sopp. pH i hver av h. 30 parts per million phenyl mercuric acetate was added to each preparation to slow down the growth of bacteria and fungi. pH in each of
■preparatene i eksemplene 1 til 27 ble holdt på 7?0. ■the preparations in Examples 1 to 27 were kept at 7?0.
5. "HAPS" - 3-(N-N-dimetyl-N-alkylammonio)-2-hydroksypropan-1-sulfonat hvori alkylgruppen er avledet fra en midlere fraksjon av kokosnottalkoholer: 2% C^ Q, 66% C2, 23% C^^og 9% C^^. 5. "HAPS" - 3-(N-N-dimethyl-N-alkylammonio)-2-hydroxypropane-1-sulfonate in which the alkyl group is derived from a middle fraction of coconut alcohols: 2% C^ Q, 66% C2, 23% C^ ^and 9% C^^.
Som det lett sees av eksempel 2 vil proteaser ("Alcalase") nedbrytes og/eller deaktiveres hurtig i vandig lbsning når det ikke er tilstede noe stabiliserende middel. Etter 3 ukers lagring ved 38°C er nær 90% av enzymaktiviteten tapt. Mindre enzymaktivitet g.ikk tapt ved de mildere betingelser, d.v.s. 10°C og 26,7°C, men tapet var meget vesentlig selv ved disse mildere betingelser. As is readily seen from Example 2, proteases ("Alcalase") will degrade and/or deactivate rapidly in aqueous solution when no stabilizing agent is present. After 3 weeks of storage at 38°C, close to 90% of the enzyme activity is lost. Less enzyme activity was lost under the milder conditions, i.e. 10°C and 26.7°C, but the loss was very substantial even at these milder conditions.
Tilsetningen av et ikke ionisk vaskeaktivt middel "TAE^q" til den vandige enzymlosning Eksempel 1 ) synes å bremse nedbrytning og/ eller deaktivering av enzymene ved lave lagringstemperaturer, men ved hoye lagringstemperaturer synes det ikke-ioniske vaskeaktive middel å oke nedbrytingshastigheten og/éller deaktiveringen av enzymene. The addition of a non-ionic detergent "TAE^q" to the aqueous enzyme solution Example 1) seems to slow down degradation and/or deactivation of the enzymes at low storage temperatures, but at high storage temperatures the non-ionic detergent seems to increase the rate of degradation and/or the deactivation of the enzymes.
Tilsetning av stabilisefingsmidlene i henhold til oppfinnelsen Addition of the stabilizing agents according to the invention
i eksemplene 3 til 23 og 25 til 27 resulterer generelt i oket enzymstabilitet ved lagringsbetingelser på 10°C. For imidlertid å oppnå vesentlig oket enzymstabilitet ved hoyere temperaturer, må de foretrukne stabiliseringsmidler, dvs. metanol, etanol, propanol, isopropanol eller 3-ProP°ksypr opand. anvendes i de tidligere beskrevne brukskonsentrasjoner. Eksemplene 5, 9, 11, 12 og 13 illustrerer den foretrukne stabiliserende virkning av de særlig foretrukne stabiliseringsmidler metanol, etanol og isopropanol, ved hoyere temperaturer (8 uker ved 38°C lagring). in Examples 3 to 23 and 25 to 27 generally results in increased enzyme stability at storage conditions of 10°C. However, in order to achieve significantly increased enzyme stability at higher temperatures, the preferred stabilizers, i.e. methanol, ethanol, propanol, isopropanol or 3-ProP°xyprop, must be added. are used in the previously described usage concentrations. Examples 5, 9, 11, 12 and 13 illustrate the preferred stabilizing effect of the particularly preferred stabilizing agents methanol, ethanol and isopropanol, at higher temperatures (8 weeks at 38°C storage).
De folgende stabiliserte vandige enzympreparater (eksemplene 28-75) ble fremstilt.. I hvert eksempel ble vann og stabiliserings-middel grundig blandet, det ikke-ioniske eller zwitterioniske vaskeaktive middel ble tilsatt og enzymene ble tilsatt til slutt. Enzymene ble stabilisert i hvert eksempel ved lagring med hoy temperatur (38°C) og under lagring ved lavere temperatur (10°C). Alle virker bra som flekkfjernere, vaskemiddeladditiver og somvaskemidler i seg selv. De ikke-ioniske ("TAE^q") og de zwitterioniske ("HAPS") additiver forbedrer lagringsstabiliteten i hvert tilfelle. Fotnoter til eksemplene 28-75. 1. "TAE^q" - et mol talgalkohol etoksylert med 30 mol etylenoksyd. 2. "HAPS" 3-(N,N-dimetyl-N-alkylammonio)-2-hydroksy-:propan-1-sulfonat. (Se fotnote 5, eksemplene 1-27). The following stabilized aqueous enzyme preparations (Examples 28-75) were prepared. In each example, water and stabilizer were thoroughly mixed, the nonionic or zwitterionic detergent was added and the enzymes were added last. The enzymes were stabilized in each example by storage at a high temperature (38°C) and during storage at a lower temperature (10°C). All work well as stain removers, detergent additives and as detergents in their own right. The nonionic ("TAE^q") and the zwitterionic ("HAPS") additives improve storage stability in each case. Footnotes to Examples 28-75. 1. "TAE^q" - one mole of tallow alcohol ethoxylated with 30 moles of ethylene oxide. 2. "HAPS" 3-(N,N-dimethyl-N-alkylammonio)-2-hydroxy-:propane-1-sulfonate. (See footnote 5, examples 1-27).
3. "Alcalase" - (se fotnote 2, eksemplene 1-2 7). 3. "Alcalase" - (see footnote 2, examples 1-2 7).
h. "Wallerstein"- a-amylase fra bakterier, porsjonsnummer 1+51+6A. 5. Hvert preparat i eksemplene 28-75 inneholder 30 deler pr. million fenylmerkuriacetat. • pH i disse preparater ble holdt på 7,0. h. "Wallerstein" - a-amylase from bacteria, batch number 1+51+6A. 5. Each preparation in examples 28-75 contains 30 parts per million phenyl mercuric acetate. • The pH in these preparations was kept at 7.0.
Eksempel 76. Example 76.
Et stabilisert vandig enzympreparat ble sammensatt fra folgende komponenter: A stabilized aqueous enzyme preparation was composed of the following components:
pH for dette preparat ble innstilt til 7,0 med natriumhydroksyd. The pH of this preparation was adjusted to 7.0 with sodium hydroxide.
Dette preparat ble anvendt i en rekke prover for flekkfjerning . This preparation was used in a number of samples for stain removal.
og vasking som angitt i det folgende. Like sterkt tilsmussete toystykker ble vasket i vandige losninger inneholdende 1 1/ k kopp av en kommersiell vaskemiddelblanding ("Tide") i 73 liter vann med hårdhetsfaktor 7 ved 5^-°C i et tidsrom av10 min. and washing as indicated below. Equally heavily soiled toy pieces were washed in aqueous solutions containing 1 1/k cup of a commercial detergent mixture ("Tide") in 73 liters of water with a hardness factor of 7 at 5^-°C for a period of 10 minutes.
Preparatene i henhold til oppfinnelsen ble anvendt "som de var", d.v.s. uten fortynning i bloteperioder på 5 min.30 min. og 3 timer. The preparations according to the invention were used "as they were", i.e. without dilution in exposure periods of 5 min.30 min. and 3 hours.
Fra omtrent ■§■ milliliter til omtrent 1 milliliter av preparatet From about ■§■ milliliters to about 1 milliliter of the preparation
ble sproytet direkte, på hvert flekket eller tilsmusset område på omtrent 5 til 10 cm 2. De tilsmussede toystykker ble behandlet i de ovenfor angitte tidsrom og ble så vasket som beskrevet ovenfor i den kommersielle vaskemiddelblanding. was sprayed directly, on each stained or soiled area of approximately 5 to 10 cm 2 . The soiled toy pieces were treated for the above time periods and then washed as described above in the commercial detergent mixture.
Preparatene ble også anvendt som et tilsetningsmiddel til kommersielle vaskemiddelblandinger. Omtrent 1,2 milliliter av preparatene i henhold til oppfinnelsen ble anvendt pr. ^,55 liter vaskevann i disse vaskinger. Resultatene er summert opp nedenunder. The preparations were also used as an additive to commercial detergent mixtures. Approximately 1.2 milliliters of the preparations according to the invention were used per ^.55 liters of washing water in these washes. The results are summarized below.
Eksempel 76 Example 76
1. Naturlig skitne T-skjorter og putetrekk-toystykker... 1. Naturally soiled T-shirts and pillowcases toys…
2. Spesielt syntetisk tilsmussede toystykker.. 2. Especially synthetically soiled toy pieces..
3. Blodimpregnerte toystykker erholdt fra det sveitsiske proveinstitutt i St. Gallen, Sveits (Eidgenossiche Material Prufungs und Mersuchsanstant FUr Industrie, Banevesen und Gewenke, Unterstrasse 11., St. Gallen, Sveits). 3. Blood-impregnated toy pieces obtained from the Swiss Testing Institute in St. Gallen, Switzerland (Eidgenossiche Material Prufungs und Mersuchsanstant FUr Industrie, Banevesen und Gewenke, Unterstrasse 11., St. Gallen, Switzerland).
h. Toystykker tilsmusset med fem forskjellige typer flekker.- h. Toy pieces soiled with five different types of stains.-
5. Rene bomullstoystykker som måler relativ fornyet avsetning. 5. Clean cotton swabs measuring relative renewed deposition.
6. Naturlig tilsmussede prøvestykker av skjortekraver. 6. Naturally soiled test pieces of shirt collars.
Den mulige gradering for protein utledes på folgende måte: The possible grading for protein is derived as follows:
Seksten separate og forskjellige proteinflekker påfores hvert prove-stykke for proteinproven. Hver flekk, etter vaskecyklusen, graderes visuit etter en skala fra 0 til 5» En gradering på 5 indikerer fullstendig fjernelse (den "mulige gradering") mens 0 indikerer ingen fjernelse. Graderinger'mellom 0 og 5 indikerer mellom-liggende partiell fjernelse. Den mulige proteingradering på 80 avledes ved å multiplisere de seksten flekker med 5 mulige punkter pr. flekk. De mulige graderinger for de andre flekker ble beregnet på samme måte. De totale graderinger viser graden av bredspektret renseevne. Sixteen separate and different protein spots are applied to each sample piece for the protein sample. Each stain, after the wash cycle, is visually graded on a scale from 0 to 5» A grade of 5 indicates complete removal (the "possible grade") while 0 indicates no removal. Grades' between 0 and 5 indicate intermediate partial removal. The possible protein grading of 80 is derived by multiplying the sixteen spots by 5 possible points per spot. The possible gradations for the other spots were calculated in the same way. The total grades show the degree of broad-spectrum cleaning ability.
Tilsetningen av preparatene i henhold til oppfinnelsen til vaske-vannet inneholdende de kommersielle vaskemidler oker graderingen (vasking med "Tide" + preparatet) like overfor vaskemiddelvaskingen (vasking med "Tide") med omtrent h0% hvilket indikerer en tydelig rensefordel. Ved å anvende en 5-min. blotlegging okes den totale gradering (5-min. blotlegging vasking med "Tide") med over 70% overfor graderingen for vasking med "Tide" mens en 30 min. eller 3 timers blotlegging oker den totale gradering med nesten 100% The addition of the preparations according to the invention to the washing water containing the commercial detergents increases the gradation (washing with "Tide" + the preparation) equal to the detergent washing (washing with "Tide") by approximately h0%, which indicates a clear cleaning advantage. By applying a 5-min. exposure increases the overall grading (5-min. exposure washing with "Tide") by over 70% compared to the grading for washing with "Tide" while a 30 min. or 3 hours of exposure increases the total grading by almost 100%
i forhold til graderingen for vasking med "Tide". in relation to the rating for washing with "Tide".
Eksempel 77 Example 77
Et munnvannspreparat ble sammensatt av folgende komponenter:"A mouthwash preparation was composed of the following components:
Enzymene er stabile over lange lagringsperioder ved relativt The enzymes are stable over long storage periods at relatively
hoye temperaturer. Blandingen er nyttig ved fjerning av tann-belegg på tennene, matpartikler fra munnhulen og slimbelegg i munnen. high temperatures. The mixture is useful for removing dental plaque on the teeth, food particles from the oral cavity and mucus in the mouth.
Eksempel 78 Example 78
Resultater omtrent tilsvarende resultatene i de foregående eksempler oppnås når de folgende enzymer eller kommersielle enzymblandinger anvendes i stedet for "Alcalase"' eller "Monsanto CRD protease (DA-10)" ved at enzymene stabiliseres i vandig losning: Subtilisin, "BPN", elastase, keratinase, karboksypeptidase, aminopeptidase, aspergillopeptidase A,'aspergillopeptidase B, collagenase, chymotrypsin, trypsin, pepsin, papain, bromelin, "Maxatase", "Protease B->+000" , Protease AP", CRD Protease", "Viokase", "Pronase-P", "Pronase-E", "Pronase-AS", Pronase-AF", "Biophase", "Rapidase P-2000", "Takamine", "BT proteolytisk enzym 2000", "Enzym L-W" , Rhozym P11" konsentrat, "Pectinol", "Rhozym PF", "Rhozym J-25" og "Amprozym 200". Results roughly equivalent to the results in the preceding examples are obtained when the following enzymes or commercial enzyme mixtures are used instead of "Alcalase" or "Monsanto CRD protease (DA-10)" by stabilizing the enzymes in aqueous solution: Subtilisin, "BPN", elastase , keratinase, carboxypeptidase, aminopeptidase, aspergillopeptidase A,'aspergillopeptidase B, collagenase, chymotrypsin, trypsin, pepsin, papain, bromelain, "Maxatase", "Protease B->+000" , Protease AP", CRD Protease", "Viokase" , "Pronase-P", "Pronase-E", "Pronase-AS", Pronase-AF", "Biophase", "Rapidase P-2000", "Takamine", "BT proteolytic enzyme 2000", "Enzyme L-W" , Rhozym P11" concentrate, "Pectinol", "Rhozym PF", "Rhozym J-25" and "Amprozym 200".
Resultater tilsvarende, som'i de foregående eksempler oppnås når folgende,stabiliseringsmidler anvendes i stedet for dem som er anvendt i de foregående eksempler for stabilisering av enzymene i vandig losning: metanol, etanol, propanol, isopropanol, butanol, isobutanol, metoksymetanol, 2-metoksyetanol, 3-metoksypropanol, 2-metoksypropanol, etoksyrnetanol, 2-etoksyetanol, 3-etoksypropanoi, 2- etoksypropanol, propoksymetanol, 2-propoksyetanol, 3-propoksy-propanol, 2-propoksypropanol, butoksymetanol, 2-butoksyetanol, 3- butoksypropanol, 2-butoksypropanol, etylenglykolmonobutyleter, dietylenglykolmonobutyleter. Results similar to those in the previous examples are obtained when the following stabilizers are used instead of those used in the previous examples for stabilizing the enzymes in aqueous solution: methanol, ethanol, propanol, isopropanol, butanol, isobutanol, methoxymethanol, 2- methoxyethanol, 3-methoxypropanol, 2-methoxypropanol, ethoxyrnethanol, 2-ethoxyethanol, 3-ethoxypropanoi, 2-ethoxypropanol, propoxymethanol, 2-propoxyethanol, 3-propoxypropanol, 2-propoxypropanol, butoxymethanol, 2-butoxyethanol, 3-butoxypropanol, 2-butoxypropanol, ethylene glycol monobutyl ether, diethylene glycol monobutyl ether.
Resultater omtrent tilsvarende, resultatene i eksempel 1 til 76 oppnås når folgende ikke-ioniske og zwitterioniske vaskeaktive midler anvendes i stedet for talgalkohol etoksyJert med 30 mol etylenoksyd pr. mol talgalkohol og 3-(N,N-dimetyl-N-middel-fraksj on-kokosnottalkylammonio)-2-hydroksypropan-1 -sulfonat ved at enzymstabiliseringen forbedres: decylfenoletoksylert med 20 mol etylenoksyd pr. mol decylfenol, heksadecansyreamid, heksadekan-syredietanol.-amid, dimetyldodecylaminoksyd, dimatyldodecyl-fosfinoksyd og dodecylmetylsulfoksyd, kondensasjonsproduktet av etylenoksyd med kondensasjonsproduktet av propylenoksyd med'propylenglykol, idet etylenoksyddélen av forbindelsen utgjor ^ 0% Results roughly equivalent, the results in examples 1 to 76 are obtained when the following non-ionic and zwitterionic detergents are used instead of tallow alcohol ethoxylated with 30 mol of ethylene oxide per mol of tallow alcohol and 3-(N,N-dimethyl-N-mid-fraction coconut alkylammonio)-2-hydroxypropane-1-sulfonate in that the enzyme stabilization is improved: decylphenol ethoxylated with 20 mol of ethylene oxide per moles of decylphenol, hexadecanoic acid amide, hexadecanoic acid diethanolamide, dimethyldodecylamine oxide, dimethyldodecylphosphine oxide and dodecylmethylsulfoxide, the condensation product of ethylene oxide with the condensation product of propylene oxide with propylene glycol, the ethylene oxide part of the compound constituting ^ 0%
av den totale vekt av forbindelsen og den samlede molekylvekt av forbindelsen er omtrent 1700, kondensasjonsproduktet av etylenoksyd med kondensasjonsproduktet av propylenoksyd og etylendiamin hvori produktet inneholder omtrent 65 vektprosent polyetylenoksyd og den samlede molekylvekt av forbindelsen er omtrent 6000. of the total weight of the compound and the total molecular weight of the compound is about 1700, the condensation product of ethylene oxide with the condensation product of propylene oxide and ethylenediamine wherein the product contains about 65 percent by weight polyethylene oxide and the total molecular weight of the compound is about 6000.
I eksemplene 3 til 2h og 26 til 76 stabiliseres enzymene i det vandige preparat når ikke-ionisk eller zwitterionisk vaskeaktivt middel er tilstede i den vandige losning. In examples 3 to 2h and 26 to 76, the enzymes in the aqueous preparation are stabilized when nonionic or zwitterionic detergent is present in the aqueous solution.
Resultater tilsvarende resultatene i eksemplene 3 til 23 og 2h til 77 oppnås når pH for preparatene holdes på 6,5, 8,0 og 8,5 ved at enzymene stabiliseres i lengre tidsrom. Enzym-stabilisering oppnås også i eksemplene 3 til 23 og 2k til 77 når pH i blandingene holdes ved henhv. 5,0, 9,0 og 10,0. Results corresponding to the results in examples 3 to 23 and 2h to 77 are obtained when the pH of the preparations is kept at 6.5, 8.0 and 8.5 by stabilizing the enzymes for a longer period of time. Enzyme stabilization is also achieved in examples 3 to 23 and 2k to 77 when the pH in the mixtures is kept at 5.0, 9.0 and 10.0.
Claims (2)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US68319667A | 1967-11-15 | 1967-11-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
NO128370B true NO128370B (en) | 1973-11-05 |
Family
ID=24742962
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO04540/68A NO128370B (en) | 1967-11-15 | 1968-11-15 |
Country Status (12)
Country | Link |
---|---|
US (1) | US3557002A (en) |
AT (1) | AT314450B (en) |
BE (1) | BE723873A (en) |
CH (1) | CH534702A (en) |
DE (1) | DE1808834C3 (en) |
DK (1) | DK132030C (en) |
FI (1) | FI49179C (en) |
FR (1) | FR1599043A (en) |
GB (1) | GB1242596A (en) |
NL (1) | NL162690C (en) |
NO (1) | NO128370B (en) |
SE (1) | SE360675B (en) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3839214A (en) * | 1969-06-27 | 1974-10-01 | United States Borax Chem | Tetraborate composition |
US3860536A (en) * | 1970-01-02 | 1975-01-14 | Cpc International Inc | Enzyme-detergent combination |
US3717550A (en) * | 1970-09-25 | 1973-02-20 | Pabst Brewing Co | Liquid compositions of bacterial protease and/or amylase and preparation thereof |
US3855142A (en) * | 1971-07-15 | 1974-12-17 | Lever Brothers Ltd | Enzymatic denture cleanser |
US4169817A (en) * | 1971-12-23 | 1979-10-02 | Midwest Biochemical Corporation | Liquid cleaning composition containing stabilized enzymes |
US3876563A (en) * | 1972-09-22 | 1975-04-08 | Procter & Gamble | Liquid detergent compositions |
US4310625A (en) * | 1976-03-17 | 1982-01-12 | Modrovich Ivan Endre | Stabilized liquid enzyme compositions for diagnostic determinations |
CA1091174A (en) * | 1976-03-17 | 1980-12-09 | Ivan E. Modrovich | Stabilized liquid enzyme |
US4277562A (en) * | 1976-09-13 | 1981-07-07 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme compositions |
US4271264A (en) * | 1976-09-13 | 1981-06-02 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme compositions |
CA1102225A (en) * | 1976-09-13 | 1981-06-02 | Ivan E. Modrovich | Stabilized liquid enzyme and coenzyme compositions and method of preparing same |
US4372874A (en) * | 1976-09-13 | 1983-02-08 | Modrovich Ivan Endre | Stabilization of hydrolysis prone labile organic reagents in liquid media |
US4250254A (en) * | 1978-09-11 | 1981-02-10 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme compositions |
US4285276A (en) * | 1978-11-15 | 1981-08-25 | Howard A. Fromson | Method for printing employing lithographic fountain dampening solution |
DE2909396B2 (en) * | 1979-03-09 | 1981-07-30 | Diamalt AG, 8000 München | Desizing agent and process for its manufacture |
US4305837A (en) * | 1980-10-30 | 1981-12-15 | The Procter & Gamble Company | Stabilized aqueous enzyme composition |
US4318818A (en) * | 1979-11-09 | 1982-03-09 | The Procter & Gamble Company | Stabilized aqueous enzyme composition |
US4287082A (en) * | 1980-02-22 | 1981-09-01 | The Procter & Gamble Company | Homogeneous enzyme-containing liquid detergent compositions containing saturated acids |
FR2520753B1 (en) * | 1982-02-01 | 1986-01-31 | Transgene Sa | NOVEL CATECHOL 2,3-OXYGENASE EXPRESSION VECTORS, ENZYMES OBTAINED AND APPLICATIONS THEREOF |
MX161813A (en) * | 1982-12-13 | 1990-12-28 | Colgate Palmolive Co | IMPROVEMENTS TO LIQUID DETERGENT COMPOSITION |
IE81141B1 (en) | 1983-06-24 | 2000-04-05 | Genencor Int | Procaryotic carbonyl hydrolases |
US4548727A (en) * | 1983-10-06 | 1985-10-22 | The Drackett Company | Aqueous compositions containing stabilized enzymes |
DE3428834A1 (en) * | 1984-08-04 | 1986-02-13 | Henkel KGaA, 4000 Düsseldorf | DISHWASHER |
DE3527913A1 (en) * | 1985-08-03 | 1987-02-12 | Henkel Kgaa | ALKALINE PROTEASE, METHOD FOR PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS |
DK563986A (en) * | 1986-11-25 | 1988-06-17 | Novo Industri As | PREPARATION OF A LOW-TEMPERATURE-ACTIVE PROTEASE |
DK564086A (en) * | 1986-11-25 | 1988-06-17 | Novo Industri As | ENZYMATIC DETERGENT ADDITIVE |
US4711739A (en) * | 1986-12-18 | 1987-12-08 | S. C. Johnson & Son, Inc. | Enzyme prespotter composition stabilized with water insoluble polyester or polyether polyol |
DE3704465C2 (en) * | 1987-02-13 | 1995-11-02 | Roehm Gmbh | Liquid formulations of enzymes |
GB8816443D0 (en) * | 1988-07-11 | 1988-08-17 | Albright & Wilson | Liquid enzymatic detergents |
US4999288A (en) * | 1987-10-28 | 1991-03-12 | Gds Technology, Inc. | Test composition and method for the determination of anilides |
DE3927286C2 (en) * | 1989-08-18 | 1997-07-24 | Roehm Gmbh | Aqueous liquid enzyme formulations |
US5156773A (en) * | 1989-12-12 | 1992-10-20 | Novo Nordisk A/S | Stabilized enzymatic liquid detergent composition |
US5030378A (en) * | 1990-01-02 | 1991-07-09 | The Procter & Gamble Company | Liquid detergents containing anionic surfactant, builder and proteolytic enzyme |
US5447649A (en) * | 1990-03-01 | 1995-09-05 | Novo Nordisk A/S | Lipase containing liquid pre-spotter and use of such pre-spotter |
DE4226162A1 (en) * | 1991-08-16 | 1993-02-18 | Sandoz Ag | STABLE ENZYMPREPAIR |
US5858117A (en) | 1994-08-31 | 1999-01-12 | Ecolab Inc. | Proteolytic enzyme cleaner |
IL117948A0 (en) * | 1995-04-18 | 1996-08-04 | Horiuchi Co Ltd | Reusable cleaning solutions containing stabilized enzymes |
PT1523553E (en) * | 2002-01-16 | 2010-03-03 | Genencor Int | Multiply-substituted protease variants |
AU2006288018B2 (en) * | 2005-09-11 | 2010-04-01 | Peter Alan Royle | Hidden fitting locator |
-
1967
- 1967-11-15 US US683196A patent/US3557002A/en not_active Expired - Lifetime
-
1968
- 1968-11-14 FI FI683261A patent/FI49179C/en active
- 1968-11-14 SE SE15454/68A patent/SE360675B/xx unknown
- 1968-11-14 DE DE1808834A patent/DE1808834C3/en not_active Expired
- 1968-11-14 FR FR1599043D patent/FR1599043A/fr not_active Expired
- 1968-11-14 AT AT1110468A patent/AT314450B/en not_active IP Right Cessation
- 1968-11-14 BE BE723873D patent/BE723873A/xx not_active IP Right Cessation
- 1968-11-15 CH CH1714868A patent/CH534702A/en not_active IP Right Cessation
- 1968-11-15 GB GB54244/68A patent/GB1242596A/en not_active Expired
- 1968-11-15 DK DK556568A patent/DK132030C/en active
- 1968-11-15 NO NO04540/68A patent/NO128370B/no unknown
- 1968-11-15 NL NL6816356.A patent/NL162690C/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
AT314450B (en) | 1974-04-10 |
NL162690B (en) | 1980-01-15 |
US3557002A (en) | 1971-01-19 |
DE1808834A1 (en) | 1969-07-17 |
NL6816356A (en) | 1969-05-19 |
FI49179C (en) | 1975-04-10 |
FR1599043A (en) | 1970-07-15 |
SE360675B (en) | 1973-10-01 |
DE1808834C3 (en) | 1980-04-24 |
GB1242596A (en) | 1971-08-11 |
BE723873A (en) | 1969-05-14 |
NL162690C (en) | 1980-06-16 |
FI49179B (en) | 1974-12-31 |
DK132030B (en) | 1975-10-13 |
DK132030C (en) | 1976-03-01 |
DE1808834B2 (en) | 1979-08-09 |
CH534702A (en) | 1973-03-15 |
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