NL2031621B1 - A eugenol time-resolved fluorescence immunochromatographic test strip and a preparation method thereof - Google Patents

A eugenol time-resolved fluorescence immunochromatographic test strip and a preparation method thereof Download PDF

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Publication number
NL2031621B1
NL2031621B1 NL2031621A NL2031621A NL2031621B1 NL 2031621 B1 NL2031621 B1 NL 2031621B1 NL 2031621 A NL2031621 A NL 2031621A NL 2031621 A NL2031621 A NL 2031621A NL 2031621 B1 NL2031621 B1 NL 2031621B1
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NL
Netherlands
Prior art keywords
eugenol
test strip
test
line
microspheres
Prior art date
Application number
NL2031621A
Other languages
English (en)
Inventor
Zhai Lu
Liu Xiaoxia
Yang Jiacheng
Jin Renyao
Original Assignee
Univ Zhejiang Gongshang
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Publication date
Application filed by Univ Zhejiang Gongshang filed Critical Univ Zhejiang Gongshang
Priority to NL2031621A priority Critical patent/NL2031621B1/en
Application granted granted Critical
Publication of NL2031621B1 publication Critical patent/NL2031621B1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2470/00—Immunochemical assays or immunoassays characterised by the reaction format or reaction type
    • G01N2470/10—Competitive assay format
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Claims (2)

CONCLUSIES
1. Een teststrip om de tijd opgeloste fluorescentie-immuniteit chromatografie van syringol snel te testen, met kenmerken van combinatiepad, testlijn en kwaliteit controleren lijn. De combinatiepad hierboven genoemd bevat de nano fluorescerende microsferen die gemerkt van Eu" - monoklonaal syringol; de testlijn bevat de drager eiwit van de geconjugeerde syringol; de kwaliteit controleren lijn bevat geit anti-muis IgG antilichaam.
2. Een manier om nano fluorescerende microsferen van syringol snel te testen met kenmerken in volgende stappen: Om nano fluorescerende microsferen die met Eu** wordt gemerkt te produceren: Om nano fluorescerende microsferen schoon te maken met MES bufferen was vloeistof, Activering van hydroxylgroep: Voeg NHS oplossing en EDC oplossing in nano fluorescerende microsferen oplossing met Eu*” merk die in de vorige stap gekregen wordt toe om te incuberen. Voeg MES bufferen oplossing toe om te resuspenderen en neerslagen; Eiwit conjugeren: Voeg de monoklonale antilichamen van syringol toe in de nano fluorescerende microsferen oplossing met Eu’ merk die in de vorige stap gekregen wordt. Dan meng het en gebruik echografie. Plaats het in de omgeving van binnentemperatuur en gebruik rotatie reactie voor 1 uur tot 3 uur; Microsferen is gesloten.
NL2031621A 2022-04-19 2022-04-19 A eugenol time-resolved fluorescence immunochromatographic test strip and a preparation method thereof NL2031621B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NL2031621A NL2031621B1 (en) 2022-04-19 2022-04-19 A eugenol time-resolved fluorescence immunochromatographic test strip and a preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL2031621A NL2031621B1 (en) 2022-04-19 2022-04-19 A eugenol time-resolved fluorescence immunochromatographic test strip and a preparation method thereof

Publications (1)

Publication Number Publication Date
NL2031621B1 true NL2031621B1 (en) 2023-01-17

Family

ID=82482789

Family Applications (1)

Application Number Title Priority Date Filing Date
NL2031621A NL2031621B1 (en) 2022-04-19 2022-04-19 A eugenol time-resolved fluorescence immunochromatographic test strip and a preparation method thereof

Country Status (1)

Country Link
NL (1) NL2031621B1 (nl)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110746286A (zh) * 2019-11-04 2020-02-04 北京维德维康生物技术有限公司 一种丁香酚半抗原、人工抗原及其制备方法与应用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110746286A (zh) * 2019-11-04 2020-02-04 北京维德维康生物技术有限公司 一种丁香酚半抗原、人工抗原及其制备方法与应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Development of a Quantitative Lateral Flow Test Using Estapor Europium Microspheres", APPLICATION NOTE, 1 May 2020 (2020-05-01), pages 1 - 6, XP093005296, Retrieved from the Internet <URL:https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/249/923/estapor-europium-microspheres-tn5922en-mk.pdf> [retrieved on 20221206] *
SHEN XINYI ET AL: "Development of a colloidal gold immunoassay for the detection of four eugenol compounds in water", FOOD AND AGRICULTURAL IMMUNOLOGY, vol. 30, no. 1, 1 January 2019 (2019-01-01), pages 1318 - 1331, XP093005195, DOI: 10.1080/09540105.2019.1687658 *

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