NL2029393B1 - Processing method for inhibiting lamb leg fat oxidation during process of air drying and fermentation - Google Patents
Processing method for inhibiting lamb leg fat oxidation during process of air drying and fermentation Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 39
- 230000003647 oxidation Effects 0.000 title claims abstract description 34
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 34
- 238000007605 air drying Methods 0.000 title claims abstract description 30
- 238000000855 fermentation Methods 0.000 title claims abstract description 30
- 230000004151 fermentation Effects 0.000 title claims abstract description 30
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 12
- 230000008569 process Effects 0.000 title abstract description 18
- 238000003672 processing method Methods 0.000 title abstract description 6
- 238000005554 pickling Methods 0.000 claims abstract description 32
- 238000001035 drying Methods 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 230000035485 pulse pressure Effects 0.000 claims abstract description 9
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 8
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 8
- 241000191948 Kocuria rosea Species 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 239000012267 brine Substances 0.000 claims abstract description 5
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims abstract description 5
- 239000008280 blood Substances 0.000 claims abstract description 4
- 210000004369 blood Anatomy 0.000 claims abstract description 4
- 210000000003 hoof Anatomy 0.000 claims abstract description 4
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- 235000020183 skimmed milk Nutrition 0.000 claims description 9
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 claims description 8
- 235000013599 spices Nutrition 0.000 claims description 8
- 239000007858 starting material Substances 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
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- 235000010288 sodium nitrite Nutrition 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
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- 108010080698 Peptones Proteins 0.000 claims description 3
- 244000269722 Thea sinensis Species 0.000 claims description 3
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/02—Preserving by means of inorganic salts
- A23B4/023—Preserving by means of inorganic salts by kitchen salt or mixtures thereof with inorganic or organic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/03—Drying; Subsequent reconstitution
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
- A23B4/22—Microorganisms; Enzymes; Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/70—Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor
- A23L13/72—Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions
- A23L13/74—Tenderised or flavoured meat pieces; Macerating or marinating solutions specially adapted therefor using additives, e.g. by injection of solutions using microorganisms or enzymes
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
Disclosed is a processing method for inhibiting lamb leg fat oxidation during a process of air drying and fermentation, which comprises the following steps: material selection and shaping: selecting a lamb rear leg, cleaning to remove blood, and removing a hoof, tendons and surplus fat; pickling: putting the lamb leg in a pulse pressure mechanical pickling pot, and mixing a pickling seasoning for pickling; inoculation: mixing cultivated Lactobacillus plantarum and Micrococcus roseus to obtain a culture mixed liquor, and then using a brine injector to inject the culture mixed liquor into the lamb leg; fermentation: putting the inoculated lamb leg in a constant - temperature and constant - humidity box for fermentation; and air drying: putting the fermented lamb leg into vacuum pulse drying equipment for air drying to prepare an air - dried and fermented lamb leg. According to the present invention, the processing depth of meat of livestock and poultry is increased; functions of nutrition and health care are increased; fat oxidation is inhibited; a taste is good; safety and sanitation are guaranteed; and a flavour is good.
Description
OF AIR DRYING AND FERMENTATION Technical Field The present invention belongs to the technical field of food processing, and in particular to a processing method for inhibiting lamb leg fat oxidation during a process of air drying and fermentation. Background Mutton is one of the widely edible meats as well as a kind of meat which is edible in each country all around the world. Besides abundant nutrients, tender flesh and easiness in digestion, the mutton can also resist cold and build up health. It is most suitable for eating in winter and thus called as a winter tonic widely popular among people. However, it has a special smell (“smell of mutton”) which influences acceptability of some consumers. As a major traditional cured meat product in China, ham is renowned in the world because of its outstanding colour, smell and taste. In pasturing areas of Xinjiang, China, folk people have a habit of air drying and maturing the pickled mutton to become air - dried mutton.
An air - dried and fermented lamb leg is a product made of a lamb leg which is successively processed by brine salting, fermentation and drying. The mutton tastes delicate and unique and is popularly among people. However, on the other hand, due to the strong smell of mutton, which is even worse when cold mutton is eaten and easy oxidation during production and processing, an unpleasing taste of rancidity is formed, which also becomes one of the factors restraining development of mutton processing. However, this problem can be overcome to a great extent by air drying and fermentation.
It is difficult and takes a lot of time to make a fermented lamb leg, while the quality is not easy to control, so that there are only several research papers about it at present, and there is no relevant product in the market.
As a high - quality livestock meat, the mutton is a nutritious food containing high protein, low fat and low cholesterol. With social development and progress, modernized research on mutton processing technologies and processing and utilization of the mutton has drawn attention from all around the world. At present, consumption modes of the mutton are only limited to direct sales of fresh products to consumers, while production of mutton products with high added values will inevitably attract more consumers.
Summary In order to overcome defects in the prior art that fat is prone to oxidation and an unpleasing taste of rancidity is formed during production and processing of lamb legs, the present invention provides a processing method for inhibiting lamb leg fat oxidation during a process of air drying and fermentation. In the method, the processing depth of meat of livestock and poultry is increased; functions of nutrition and health care are increased; fat oxidation is inhibited; a taste of a finished product is good; safety and sanitation are guaranteed; and a flavour is good. To solve the technical problems, the present invention adopts the following technical solution: A processing method for inhibiting lamb leg fat oxidation during a process of air drying and fermentation, wherein the method comprises the following steps: (1) material selection and shaping: selecting a lamb rear leg, cleaning to remove blood, and removing a hoof, tendons and surplus fat; (2) pickling: putting the lamb leg in a pulse pressure mechanical pickling pot, and mixing a pickling seasoning for pickling; (3) inoculation: mixing cultivated Lactobacillus plantarum and Micrococcus roseus to obtain a culture mixed liquor, and then using a brine injector to inject the culture mixed liquor into the lamb leg; (4) fermentation: putting the inoculated lamb leg in a constant - temperature and constant - humidity box for fermentation; (5) air drying: putting the fermented lamb leg into vacuum pulse drying equipment for air drying to prepare an air - dried and fermented lamb leg.
Preferably, step (2) comprises: uniformly smearing the pickling seasoning on the surface of the lamb leg, then starting the pulse pressure mechanical pickling pot to pickle the lamb leg, keeping a high pressure of 150 - 160 kpa for 6 - 8 min at first, then recovering to an atmospheric pressure and keeping the pressure for 20 - 30 min, and repeating in this way for 20 - 24 h; and based on the mass of the lamb leg, the pickling seasoning comprises an antioxidant of 0.2 - 0.4% by mass of the lamb leg, salt of 4 - 5%, Sichuan pepper powder of 0.2 - 0.3%, star anise of 0.2 -
0.3%, five spice powder of 0.05 - 0.10%, cooking wine of 3 - 5% and white granulated sugar of
0.5- 1.5%. The use of high pressure and normal pressure cycles is beneficial to permeate the pickling liquid into the meat and realizes fast and obvious flavouring. Pickling time needed under normal pressure, which is about 3 - 5 days, is shortened to 1 day, and the decrease in the pickling time can, to great extent, control fat oxidation; addition of the antioxidant can effectively prevent fat oxidation during processing, and in particular, EDTA is added to complex metal ions in meat so as to effectively control oxidation; and the pickling seasoning can enhance smell, colour and taste.
Preferably, in step (2), the antioxidant is prepared through dissolution of multiple components in water; and the mass fraction of each component in the antioxidant is as follows: 0.03% of EDTA,
0.2% of tea polyphenol, 0.1% of red date flavone and 0.01% of sodium nitrite. EDTA is a complexing agent which can effectively complex iron ions in mutton and reduce the content of iron ions in the mutton. As shown in former studies, through addition of metal ions including Fe®* and Fe? with different concentrations for processing of lamb fat and storage at 4+1°C, duration of oxidation induction time of a fat sample processed by the different concentrations of Fe** and
Fe?" keeps a trend of gradual decrease (P< 0.05); processing of Fe?’ and Fe? can accelerate oxidation and decomposition of fat; and an oxidation rate of the lamb fat in a Fe?* treatment group is quicker.
Through processing of mutton with EDTA, fat oxidation can be effectively inhibited.
Tea polyphenol and flavones are highly anti - oxidation; and addition of sodium nitrite can enhance colour, resist infectious microbes (with targeted inhibition to Clostridium botulinum) and can also improve the flavour of the meat product.
Preferably, in step (3), the Lactobacillus plantarum is obtained through starter propagation of a skim milk culture medium, and the Micrococcus roseus is obtained through starter propagation of a beef infusion culture medium; preparation of the skim milk culture medium: conducting sterilization of a 12% skim milk powder solution for 20 min at 121°C and 0.1MPa, putting the solution in a 30°C incubator for culture of 3 d after quick cooling, and starting application after sterility is proved for activation of the Lactobacillus plantarum and preparation and production of a starter; preparation of the beef infusion culture medium: taking a beef infusion of 1000ml, peptone of 10 g, sodium chloride of 5 g and agar of 20 - 25 g at pH of 7.2 - 7.4; and preparation of the beef infusion: cleaning and chopping lean beef, weighing 550 g and soaking in water of 1375 ml for 12 h, and filtering and conducting sterilization for 40min at 121°C and 0.1 MPa to prepare the beef infusion.
Under effects of microbes such as lactic acid bacteria, sugar in the meat product can be transformed to acids or alcohals, so that the pH value is reduced, growth of pathogenic microorganisms and putrefying bacteria is inhibited and a shelf life is prolonged; and meanwhile, enzymes such as protease and lipase can be generated during fermentation, and protein is broken up to polypeptides and amino acids prone to absorption by human bodies, so that nutrition values of the product are increased.
Preferably, in step (4), the fermentation temperature is 30°C and the humidity is 93% in the constant - temperature and constant - humidity box, the temperature and humidity are adjusted when the pH value decreases to about 5.0 for a maturation stage, and the temperature is controlled at 8°C and the humidity is controlled at 80% till the moisture content in the lamb leg decreases to 70 - 80%; and fermentation time is determined according to the pH value in the meat product, and fermentation ends when the pH decreases to about 5.0. Two gradients represent two processes, namely a fermentation process and a maturation process.
The fermentation stage aims to produce acids and reduce the pH value; and in the maturation stage, the temperature and humidity are reduced, culture is inhibited from keeping growth, and a flavour substance is generated under effects of various enzymes.
Preferably, step (5) comprises: putting the mature lamb leg in the vacuum pulse drying equipment for air drying, keeping for 5 - 10 min under a vacuum degree of - 0.05 to - 0.08 Mpa, and then keeping 20 - 30 min under a normal pressure, repeating in this way for 96 - 120 h and keeping the temperature of 30 - 40°C in the vacuum pulse drying equipment.
During the process of air drying, a vacuum pulse pressure technology is used; vacuum and normal pressure act alternately to make moisture in the meat migrate quickly and evaporate quickly; and the time for the air drying process is shorter than conventional air drying time. During the vacuum process, there is no contact with oxygen, so that fat oxidation is effectively inhibited; and in addition, decrease of the air drying time also reduces occurrence of oxidation.
The present invention has the advantages that: the processing depth of meat of livestock and poultry is increased; functions of nutrition and health care are increased; fat oxidation is inhibited; the taste is good; safety and sanitation are guaranteed; and the flavour is good.
Description of Drawings Fig. 1 is a comparison diagram of changes in a peroxidation value between a new method in embodiment 1 and a traditional method during a process of lamb leg processing; and Fig. 2 is a comparison diagram of changes in TBARS between a new method in embodiment 1 and a traditional method during a process of lamb leg processing.
Detailed Description The present invention will be further described below in conjunction with accompanying drawings: Embodiment 1:
1. Production of a lamb leg: (1) material selection and shaping: selecting a lamb rear leg, cleaning to remove blood, and removing a hoof, tendons and surplus fat; (2) preparation of a pickling seasoning: weighing an antioxidant of 0.3% by mass of the lamb leg, salt of 4%, Sichuan pepper powder of 0.2%, star anise of 0.3%, five spice powder of 0.08%, cooking wine of 4% and white granulated sugar of 1%, and stirring and mixing uniformly to obtain the pickling seasoning, wherein the antioxidant is prepared through dissolution of multiple components in water; and the mass fraction of each component in the antioxidant is as follows:
0.03% of EDTA, 0.2% of tea polyphenol, 0.1% of red date flavone and 0.01% of sodium nitrite; (3) pickling: uniformly smearing the pickling seasoning on the surface of the lamb leg, then starting a pulse pressure mechanical pickling pot to pickle the lamb leg, keeping a high pressure of 150 kpa for 7 min at first, then recovering to an atmospheric pressure and keeping the pressure for 20 min, and repeating in this way for 22 h; (4) preparation of culture media: preparation of a skim milk culture medium: conducting sterilization of a 12% skim milk powder solution for 20 min at 121°C and 0.1 MPa, putting the solution in a 30°C incubator for culture of 3 d after quick cooling, and starting application after sterility is proved; preparation of a beef infusion culture medium: cleaning and chopping lean beef, weighing 550 g and soaking in water of 1375 ml for 12 h, and filtering and conducting sterilization for 40 min at 121°C and 0.1 MPa to prepare a beef infusion, and then taking the beef infusion of
1000 ml, peptone of 10 g, sodium chloride of 5 g and agar of 20 - 25 g and controlling the pH to be 7.2 - 7.4 to prepare the beef infusion culture medium; (5) culture starter propagation: Lactobacillus plantarum is treated by starter propagation by the skim milk culture medium, and the Micrococcus roseus is treated by starter propagation by 5 the beef infusion culture medium; (6) inoculation: mixing the cultivated Lactobacillus plantarum and Micrococcus roseus to obtain a culture mixed liquor, and then using a brine injector to inject the culture mixed liquor into the lamb leg; (7) fermentation: putting the inoculated lamb leg in a constant - temperature and constant - humidity box for fermentation, wherein the fermentation temperature is 30°C and the humidity is 93%, the temperature and humidity are adjusted when the pH value decreases to about 5.0 for a maturation stage, and the temperature is controlled at 8 - 10°C and the humidity is controlled at 80% till the moisture content in the lamb leg decreases to 70 - 80%; (8) air drying: putting the mature lamb leg into vacuum pulse drying equipment for air drying, keeping for 8 min under a vacuum degree of - 0.06Mpa, and then keeping 25 min under a normal pressure, repeating in this way for 110 h and keeping the temperature of 35°C in the vacuum pulse drying equipment so as to prepare an air - dried lamb leg.
2. Sample Characterization: Fig. 1 shows a comparison diagram of changes in a peroxidation value between a new method in embodiment 1 and a traditional method during a process of lamb leg processing; and Fig. 2 shows a comparison diagram of changes in TBARS between a new method in embodiment 1 and a traditional method during the process of lamb leg processing.
As shown in comparison experiments, processing time in embodiment 1 is 8d shorter than traditional processing time; and indexes of a processing process and finished product oxidation are obviously decreased. A peroxidation value and TBARS are important indexes of an oil oxidation degree. As shown in comparison results, the peroxidation value of the finished product processed by embodiment 1 is 3.7, and is 4.8 in the contrast experiment; and the value of TBARS is 0.75, and the contrast is 1.81, showing a very significant difference (P<0.01). The oxidation indexes are obviously lower than those of the contrast, indicating that the method of the present invention can effectively inhibit fat oxidation of the air - dried and fermented lamb leg and has obvious effects.
Embodiment 2:
1. Production of a lamb leg: Based on embodiment 1, the present embodiment only adjusts technological processes in steps (2), (3) and (8) of embodiment 1 and non - stated parts are completely consistent with embodiment 1, which will not be repeated in the present embodiment.
(2) Preparation of a pickling seasoning: weighing an antioxidant of 0.4% by mass of the lamb leg, salt of 5%, Sichuan pepper powder of 0.3%, star anise of 0.3%, five spice powder of 0.10%, cooking wine of 5% and white granulated sugar of 1.5%, and stirring and mixing uniformly to obtain the pickling seasoning; (3) pickling: uniformly smearing the pickling seasoning on the surface of the lamb leg, then starting a pulse pressure mechanical pickling pot to pickle the lamb leg, keeping a high pressure of 160 kpa for 8 min at first, then recovering to an atmospheric pressure and keeping the pressure for 30 min, and repeating in this way for 24 h; (8) air drying: putting the mature lamb leg into vacuum pulse drying equipment for air drying, keeping for 10 min under a vacuum degree of - 0.08 Mpa, and then keeping 30 min under a normal pressure, repeating in this way for 120 h and keeping the temperature of 40°C in the vacuum pulse drying equipment so as to prepare an air - dried lamb leg.
2. Sample Characterization: As shown in comparison experiments, characterization performance of the product prepared by embodiment 2 is consistent with that of embodiment 1. The oxidation indexes are obviously lower than those of the contrast, indicating that the method of the present invention can effectively inhibit fat oxidation of the air - dried and fermented lamb leg and has obvious effects, which will not be repeated in the present embodiment.
Embodiment 3:
1. Production of a lamb leg: Based on embodiment 1, the present embodiment only adjusts technological processes in steps (2), (3) and (8) of embodiment 1 and non - stated parts are completely consistent with embodiment 1, which will not be repeated in the present embodiment.
(2) Preparation of a pickling seasoning: weighing an antioxidant of 0.2% by mass of the lamb leg, salt of 4%, Sichuan pepper powder of 0.2%, star anise of 0.2%, five spice powder of 0.05%, cooking wine of 3% and white granulated sugar of 0.5%, and stirring and mixing uniformly to obtain the pickling seasoning; (3) pickling: uniformly smearing the pickling seasoning on the surface of the lamb leg, then starting a pulse pressure mechanical pickling pot to pickle the lamb leg, keeping a high pressure of 150 kpa for 6 min at first, then recovering to an atmospheric pressure and keeping the pressure for 20 min, and repeating in this way for 20 h; (8) air drying: putting the mature lamb leg into vacuum pulse drying equipment for air drying, keeping for 5min under a vacuum degree of - 0.05 Mpa, and then keeping 20 min under a normal pressure, repeating in this way for 90 h and keeping the temperature of 30°C in the vacuum pulse drying equipment so as to prepare an air - dried lamb leg.
2. Sample Characterization:
As shown in comparison experiments, characterization performance of the product prepared by embodiment 3 is consistent with that of embodiment 1. The oxidation indexes are obviously lower than those of the contrast, indicating that the method of the present invention can effectively inhibit fat oxidation of the air - dried and fermented lamb leg and has obvious effects, which will not be repeated in the present embodiment.
Finally, it should be noted: apparently, the above embodiments are only for clearly illustrating examples made by the present invention, rather than limiting the implementation. Those ordinary skilled in the art may make other different forms of changes or variations based on the above illustration. It is not necessary or possible to list all the implementations. Apparent changes or variations derived therefrom still fall into the protection scope of the present invention.
Claims (6)
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