MXPA99001131A - Antiprotozoal composition - Google Patents
Antiprotozoal compositionInfo
- Publication number
- MXPA99001131A MXPA99001131A MXPA/A/1999/001131A MX9901131A MXPA99001131A MX PA99001131 A MXPA99001131 A MX PA99001131A MX 9901131 A MX9901131 A MX 9901131A MX PA99001131 A MXPA99001131 A MX PA99001131A
- Authority
- MX
- Mexico
- Prior art keywords
- fermentation
- rumen
- soluble
- glutamic acid
- corn
- Prior art date
Links
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Abstract
The invention relates to a composition and compound with antiprotozoal activity useful for the control of protozoal populations under a variety of conditions, particularly in ruminants. The composition and compound of the invention may be used as a feed supplement in a diet formulated for ruminant animals.
Description
COMPOSITION ANTI-PROTOZOARIA COMPENDIUM OF THE INVENTION The invention relates to a composition and a compound with an anti-protozoal activity. These materials will enhance the control of protozoan populations under a variety of conditions, particularly ruminants. In a preferred embodiment of the invention, the composition and compound, when used as a dietary supplement in a diet formulated for ruminant animals, will significantly produce the protozoan populations in ruminants, although the use of the materials of the invention to control protozoan populations is not restricts only ruminant control. In this application it has been shown that the control of protozoa in ruminants significantly increases feed intake and increases the digestive efficiency of ruminants. In a preferred embodiment of the invention the source of the anti-protozoan compound is derived from the cell bodies of bacterial species, preferably Corynebacterium. Another source is Brevibacterium. The preferred species are lactofermentum and glutamicum. The anti-protozoal activity is found in cell bodies isolated by themselves in the materials of by-products derived from the fermentations derived from these bacteria in which cell bodies can be present (soluble in fermentation). The examples are soluble by fermentation of glutamic acid and soluble by fermentation of corn. In ruminant animals, the bodies of the cells and / or byproducts of the fermentation can be fed unprocessed (not dry), although the maximum response is achieved when the cell bodies and / or by-products are dried at temperatures that naturalize the components of organic nitrogen present in it. This temperature varies with the drying method used and generally ranges from not less than about 26.6 to not more than about 482.2 degrees centigrade. Background of the Invention The normal diet of the ruminant animal is forage. The forage includes fats, legumes and cellulitic byproducts of agricultural production. These are foods either fresh as fodder or green cuts; in a dry form such as hay; a state conserved as ensilage. The ability to use these materials as nutrient sources is only possible as a result of pregastric bacterial fermentation in the rumen, the non-fundal portion of the animal's stomach. Here, bacterial action reduces complex structural carbohydrates; cellulose, hemicellulose and lignin and the associated non-structural carbohydrates; pectin, starches and sugars, for fatty acids or chemically simpler forms of carbohydrates, which are then subjected to gastric action in the fundus stomach and small intestine. The adaptation of ruminants to pregastric digestion has involved a system of retention of digestions, which is an essential part of the mechanism for maximal energy extraction.
This retention requires some sacrifices in food consumption, which is more limited in diets based on forage, because the larger intake should be retained longer to achieve efficient extraction of energy. This is a special problem in the modern domesticated ruminant, in that the nutrient demands created by genetic selection for fast growth of lean muscle or high levels of milk production greatly exceed the supply generated by rumenal fermentation in diets. based on forage. The diets that must be fed require the addition of large amounts of non-structural carbohydrates (starches and sugars) fed in the form of grains which, unfortunately, is often a source of physiological and metabolic stress. These problems are associated with the changes that occur in rumenal fermentation as the result of ingestion of grains. Due, feeding strategies should try to maximize the use of the forage while not compromising the supply of nutrients required for maintenance and production. A solution to the problem of nutrient supply and demand in the ruminant animal, as imposed by the limitations of bacterial pregastric digestion, is to increase the efficiency and regime in which this process occurs. The rumen is a continuous fermentation system that is supplied with nutrients (food), regulatory solutions (salivary and other salts) and fluids (water and saliva) on a continuous and intermittent basis. The efficiency of this fermentation is measured through the production of the rumen. Production is conventionally expressed as the portion of the rumen content that leaves the rumen per hour. Liquids and solids are produced at different regimes but usually related. It has been found that fluid flow regimes, such as proportions of total liquid volume, are produced at regimens that increase from <8 to 13.5% / hour as dry matter consumption of 5 to 21 kg / day (Livestock Prod. Sci., 17:37, 1987). At the same time, the production of solids increases from 3 to 5% / hour due to increased consumption. In other studies, values of 17% / hour for liquids were reported (Can. J. Ani. Sci .. 64 (Supp.): 80, 1984) and as high as 7.0% / hour for concentrates (J. Dairy Sci. ., 65: 1445, 1982). In a normal ration of a dairy cow that consumes > 20 kg of dry matter / day, the representative rumen digestion step regimes could be 15% / hour for liquids, 6% / hour for grains and 4.5% / hour for forages. The regimes could increase with a lower level of consumption. Another important feature of the rumen associated with the production regime is the microbial yield, where the microbial yield is defined as the amount of microbial mass that flows from the rumen per day. An additional and important refinement of this expression of microbial yield, which is also effected by the production regime, is the efficiency of microbial yield. This is usually expressed as microbial protein (or nitrogen) grains produced per kg. of organic matter (MO) digested in the rumen. Both aspects of microbial production have applied significance. Microbial yield is important as an index of the amount of microbial protein available to the ruminant animal per day. Microbial efficiency is important as part of the microbial yield calculation where: microbial yield (microbial N / day) = microbial efficiency (microbial N / kg digested organic matter) x kg of MO digested in the rumen day. Due to the regimes of rapid production of rumen found in cattle with high dry matter consumption, such as cattle, high microbial efficiencies are expected. However, if there is an imbalance in the nutrients available to the rumen microbes, the microbial efficiency can be damaged. This is particularly evident if ruminally available nitrogen or carbohydrate sources are inadequate. Another factor that affected the microbial efficiency of ruminants and yield is predation by rumen protozoa. The rate of bacterial predation is proportional to the concentration of available bacteria. Coleman (The Roles of Protozoa and Fungi in Ruminant Digestion, Armidale, Penambul Books, 1989, p.3) reported that when bacterial concentrations were 109 / ml. a representative value of the same in rumen fluid, the average absorption for 18 species of protozoa was 493 bacteria / hour / protozoa; when the bacteria were at maximum density, the average absorption for the 18 species of protozoa was 3.739 bacteria / hour / protozoa. Predation of protozoa involves the absorption that usually kills the bacteria. The overall effect of predation on numbers of bacteria is considerable. It has been shown that the removal of protozoa (defaunation) can result in a 2-4 fold increase in the numbers of rumen bacteria. The reduction in bacterial numbers is not uniform across all species. Generally, more amylolitic than cellulolytic bacteria are absorbed. It is possible, therefore, to conclude that the degree and digestion regime of several carbohydrates differ between animals with fauna and desfaunados. Protozoa also have negative effects on rumen function. Due to its sequestration in large feeding particles and on the rumen wall, the flow of rumen protozoa is less than could be expected from its concentration in the rumen and digestion flow regime. Therefore, although protozoa may represent 50% of the biomass in the rumen, they contribute 20% or less of the microbial protein flowing into the duodenum. In addition, predation on bacteria causes the recycling of the bacterial protein in the rumen. Protozoarians absorb and kill large amounts of bacteria, assimilating most of the protein of these organisms. Since most protozoa remain in the rumen until they are lysed, the flow of rumen microbial proteins is also reduced. The in vivo measurements summarized by Jounay and others (Anim. Feed Scí. Tech. 21: 229, 1988), indicate that the defaunation resulted in a 36% increase in grams of microbial nitrogen flowing from the rumen per kg of organic matter. Fermented An additional negative aspect of protozoa on digestive function that is important for ruminants in general, and in particular for cows with high milk production, is their absorption and digestion of food protein in particular. This allows protozoa to assimilate proteins from the low rumen degradation that has been added to the diet as sources of bypass protein (Hoover et al., Rumen Digestive Physiology and Microbial Ecology, West Virginia University Bulletin 708T; p 22.). The overall effects of the presence or absence of protozoa in ruminants has not been characterized due to the difficulty of reducing their numbers in vivo. Compounds that have demonstrated defaunation activity have proven to be very toxic to safe elements for ruminants or that are ineffective when fed for prolonged periods. Therefore, although the benefits of defaunation have been demonstrated conclusively in vitro, the transfer of this information to field practice has yet to be achieved. OBJECTIVES OF THE INVENTION In view of the deficiencies of the technique as described above and other related goals well known in the art, an objective of the present invention is to provide a feed additive that when used as a component in diets for ruminants, will control, reduce and avoid protozoan populations in ruminants. Another objective of the present invention is to provide a feed additive which allows the control, reduction and prevention of protozoa populations and thus allow the increase of ruminal microbial growth and efficiency, the benefits of which increased the flow of the microbial protein to the duodenum, increased the efficiency of ruminal fermentation and increased the consumption of dry matter. DETAILED DESCRIPTION OF THE INVENTION The above objectives have been obtained by the discovery of a composition comprised of the final liquid streams of the fermentations derived by the species Corynebacterium and / or Brevibacterium. These extreme streams can be characterized as having approximately 40% solids in the composition. The solids are comprised of nitrogen without protein, peptides and amino acids and any residual unspent carbohydrate that remains from fermentation. The predominant source of both peptides and amino acids are the lysed cell bodies of the bacterial species already referred to. In the preferred embodiment of the invention, this composition is obtained from fermentation soluble glutamic acid, corn fermentation soluble or a mixture thereof or the isolated cell bodies themselves, although any extreme fermentation stream having these can be used. components and is derived from the fermentations produced by the bacterial species Corynebacterium and / or Brevibacterium. This composition can be dried or left in liquid form. If it dries, the drying should occur at temperatures that will not denature the organic components of the composition. A vehicle such as wheat intermediate product, etc. may be included, if desired, and in view of the drying method used. Any amount of vehicle can be used. The normal scales (w / w) of dry soluble to intermediate wheat products are from 10 to 1 to 1 to 10 including all the values and sub-scales between them. The vehicle is preferably edible by the ruminant and preferably is a common food ingredient. Any of these soluble or cell bodies isolated alone or individually, mixed in a vehicle, if necessary, are suitable. When the fermentation solubles of corn and glutamic acid are mixed, or the soluble and the isolated cell bodies are mixed, they are mixed in any proportion, either before or after drying and each one on a vehicle optionally, if it is mixed in dry. The compositions described in USSN 08 / 486,226, incorporated herein by reference, may also be used herein.
If one or more soluble and cell bodies are dried, they are brought to a moisture content of about 30% at a low temperature. The moisture content of 0%, 8%, 14%, etc., is acceptable. The drying methods that can be used include vacuum drying, direct and indirect heat application, spray drying, evaporation, etc. A forced air grain processor in some manner useful for roasting soybeans is preferred for the soluble or soluble mixtures, or mixtures of the soluble and isolated cell bodies. Vacuum drying is preferred for cell bodies alone. Regardless of the method used, drying must be done at temperatures that do not denaturate the nitrogen fractions. The present inventor has provided a composition and active compound with an anti-protozoal activity derived in the preferred embodiment of the invention from fermentation soluble glutamic acid and / or fermentation soluble corn and / or cell bodies "isolated from the bacterium Corynebacterium and Brevibacterium to which a carrier of said dry materials can be added, which is superior to the prior art compositions.Corynebacterium and Brevibacterium are facultative anaerobes used in a number of industrial fermentation processes.Examples are the production of amino acids such as lysine (Brevibacterium ilium) and glutamic acid (Corynebacterium ilium; Corynebacteria glutamicum) It is the opinion of some taxonomists that Cornyeabacterium and Brevibacterium are, however, the same organism and the current distinction comes from improvements in classification techniques. end of the fermentations driven by e These organisms are usually liquid tributaries that have more than 30% by weight of water (moisture), normally 50-60%. Commonly they contain nitrogen without proteins, peptides and amino acids derived in a large part of the cell bodies lysed from the organisms to which they are referred and may contain small amounts of residual carbohydrates that remain from the fermentations themselves. The examples are Solubles of Fermentation of Glutamic Acid and Solubles of Fermentation of Corn. The cultivation of these organisms and their industrial use for the production of amino acids are a well known manufacturing process and as the nomenclature of extreme currents are defined to be determined by the Association of American Feed Control Officials. The Glutamic Acid Fermentation Solubles are comprised of a combination of water, nitrogen without protein, mainly in the form of ammonium chloride, peptides and free amino acids derived mainly from the hydrolysis of the microorganisms used to produce the fermentation, glutamic acid and inorganic salts such as MgSO4, NaCI and KH2P0. The Solubles of Fermentation of Corn are comprised of a combination of water, nitrogen without protein, mainly in the form of ammonium sulfate, peptides and free amino acids derived from the hydrolysis of the microorganisms used to produce the fermentation and the inorganic salts such as MgSO4, NaCl and MnS0 Cell bodies isolated from bacteria are comprised mainly of peptides and free amino acids, although some nitrogen without protein may be present as an artifact. The invention is useful as a feed additive in any diet for ruminants. The compound when fed to ruminant animals significantly reduces (100%, 95%, 90%, 85%, 75%, 65%, 50%, 25%, 10% and 5% and less while still providing reduction depending on the program of dose and feeding) of protozoan populations of ruminants (numbers). The net result at one to low percentage reductions is a significant increase in bacterial populations of resident ruminants. This allows improved microbial fermentation efficiency, improved ruminal microbial performance; increased supply of microbial protein to the duodenum and increased rumen production of ingested food products. The food supplement of the invention can be added to any food given to ruminants, preferably to foods comprising at least one of grass, legumes, corn or silage of small grains or hay, by-products of grain, oily seeds and oily seed meal, corn grain, and small grains, etc., to provide a supplemented food The aggregate amount will generally vary from 0.50 grams to 250 grams of cell bodies isolated alone and from 250 grams to 1 kg of soluble or soluble mixtures or mixtures of soluble and cell bodies isolated per head per day, depending on the species that they will be fed. The above objects are also met by a composition comprised of dry free amino acids, peptides, organic and inorganic nitrogen as well as structural (fiber) and non-structural carbohydrates as necessary. In the preferred embodiment of the invention, this composition is obtained from fermentation solubles of glutamic acid, corn fermentation solubles or a mixture thereof, although any source material which provides the components of these fermentation solubles can be used. The origin of the base materials is not important. It is that they provide the components of the fermentation solubles of corn and / or glutamic acid. A vehicle such as wheat intermediate products can be included if desired and in view of the drying method used. Any of these soluble alone or individually, mixed in a vehicle if necessary, are suitable. When the fermentation solubles of corn and glutamic acid are mixed, they are mixed in any proportion, either before or after drying and each optionally on a vehicle if mixed dry. The solubles of the invention (mixture or other form) are dried at a maximum moisture content of about 30% at a low temperature. The moisture contents of 0%, 8%, 14%, etc., are acceptable. The drying methods that can be used include vacuum drying, direct and indirect heat application, spray drying, evaporation, etc. A forced air grain processor somehow useful for roasting soybeans is preferred. Regardless of the method used, the drying should be checked at temperatures that will allow the modification of the solubility of the nitrogen fractions without denaturing them. To at least one of the fermentation solubles of corn or glutamic acid, one or a combination of cellulitic and / or amialytic enzymes of any bacterial or fungal origin and an amino acid such as glutamic acid can be added to increase the effect biological. Glutamine can be used as a substitute for, or together with, glutamic acid. These materials can be added either before or after drying. Generally, these components total a total of about 4 to about 10% by weight of the final composition. The preferred amounts of the enzyme vary from 15-60 grams (2% to 4%) of xylanase (75,000 units of xylanase per gram) and 20 cellulose (100,000 units of endocellulose per ml). The preferred amounts of glutamic acid vary from 0.70 to 4.0 grams (0.07 to 0.02). The present inventor has provided a mixed source of organic and inorganic nitrogen of varying solubilities in the form of nitrogen without protein, peptides, amino acids and intact protein derived in the preferred embodiment of the invention from fermentation soluble glutamic acid and / or fermentation soluble of corn to which a vehicle can be added, additional amino acids and enzymes and which is superior to the compositions of the prior art. >;
The glutamic acid fermentation solubles and corn fermentation solubles are the liquid effluents of the bacterial fermentative processes used to produce monosodium glutamate and lysine hydrochloride, respectively. These processes are well-known and common manufacturing processes and the nomenclature by which these products are defined has been determined by the Association of American Feed Control Officials. The Glutamic Acid Fermentation Solubles are comprised of a combination of water, nitrogen without protein, mainly in the form of ammonium chloride, peptides and amino acids derived from the hydrolysis of the microorganisms used to produce the fermentation, glutamic acid and inorganic salts such as MgSO4, NaCI and KH P04. The Solubles of Fermentation of Corn are comprised of a combination of water, nitrogen without protein, mainly in the form of ammonium sulfate, peptides and free amino acids derived from the hydrolysis of the microorganisms used to produce the fermentation and inorganic salts such as MgSO, NaCl and MnS04. Any enzyme capable of reducing the tissue of plants such as proteins, starches, sugars, pectins, cellulose, hemicellulose and lignin, are suitable for use in this invention. Examples of such enzymes are proteases, amylases, dextranases, pectinases, cellulases, xylanases, mannanases and ligninases. These can be of bacterial or fungal origin. You can use their mixtures. Any amino acid can be added to the mixture of the invention described above, although glutamic acid is used in the preferred embodiment of this invention. The invention is also useful as a feed additive in any diet of ruminants. As a result of processing (i.e., drying at a moisture content of up to 30% by weight, optionally in a vehicle), the non-protein nitrogen fractions of the composition of the invention, normally highly soluble in the rumen environment, are they reduce their solubility so that they provide rumen bacteria with a source of sustained release of ammonium nitrogen. The nitrogen components without protein are further complemented by the peptides and intact protein that provide the composition of the invention, the net result being a feed additive which provides a stable state of nitrogen which significantly increases the microbial efficiency of the rumen and the microbial performance. This effect can be further increased by the addition of an amino acid similar to glutamic acid, which, when added to the composition of the invention will supply the animal with the amino acid such as glutamic acid at a rate not less than 0.08 grams / kg of dry matter consumption per day. The addition of one or more proteolytic, amialitic and celuiitic enzymes also increases the net response allowing the synchronization of nitrogen sources with carbohydrates, thus ensuring the availability of sufficient amounts of energy for the synthesis of increased microbial proteins that is stimulated by the Modified nitrogen fractions. The food supplement of the invention can be added to any feed given to ruminants, preferably to foods comprising at least one of, grass, legumes, corn, small-grain silage or hay, grain by-products, oilseeds and meal. oily seeds, corn grain, and small grains, etc., to provide a supplemented food. The amount added when used as a food supplement will generally vary from about 0.225 to about 5.4 kilograms per head per day, depending on the application and species to be fed. Having generally described this invention, further understanding can be obtained by reference to certain specific examples that are provided herein for purposes of illustration only and are not intended to be limiting. Examples Preparation of Ruminant Food Supplement In this example, the drying method used is forced air, although any process that allows the removal of excess moisture while not damaging the biological value of the nitrogen components of the invention can be used, with the necessary modifications in the preparation of materials as dictates by the method used. EXAMPLES Preparation of Ruminant Food Supplement in a Dry Form Although the food supplement can be used in its unprocessed liquid form, in this example the food supplement is prepared using a mixture of glutamic acid fermentation soluble and corn fermentation soluble which is Dry on a vehicle, using forced air, although you can use any process that allows the removal of excess moisture while not damaging it. Preparation of Food Supplement for Isolated Cellular Body Ruminants In this example the food supplement is prepared from bacterial cell bodies. The final currents of the fermentations derived from the bacteria referred to, are centrifuged at speeds that allow the adequate separation of the different phases based on the specific gravity and density. Other methods that are applicable include, but are not restricted to, evaporation, membrane filtration, diffusion, ion exchange and precipitation. The cell bodies or cell cream are then dried using any method that will not denature the nitrogen fractions of the present.
The Effect of Ruminant Feed Additive on Protozoan Populations of Ruminants As Measured by In Vitro Rupture of Selenomonas ruminatium labeled with leucine (14C). Example 1 A series of in vitro experiments were carried out to determine the anti-protozoal activity of the ruminant feed additive using the method as described by Wallace and McPherson (British Journal of Nutrition 58, 313-323) and Wallace and Newbold (Journal of Agricultural Science, Cambridge, 116, 163-168). In this method, the protozoal activity was measured by the rupture of Selenomonas ruminantium labeled with leucine (1 C). The rumen fluid, recovered from mature sheep with probe in the rumen, is filtered through 2 layers of muslin and pre-incubated at 39 ° C with a mixture (40 g / L) of the feed additive of the invention with a straw of wheat (0, 1, 10, 25 and 50 grams of the feed additive of the invention) for 2 hours before adding S. ruminantium. An unlabeled L-leucine was included in all incubations at a final concentration of 5 mmol / L to avoid the reincorporation of leucine (14C) released. In this experiment, the food additive of the invention was a mixture of liquid glutamic acid fermentation soluble and corn fermentation soluble mixed at a ratio of 60/40 w / w, dried in a wheat intermediate product vehicle in a 50/50 weight basis. The drying method used was forced air. The results of this experiment are summarized in Table 1. As the concentration of the feed additive of the invention was increased from 1 gram per liter to 50 grams per liter, the rupture of S. ruminantium as a result of the predation of protozoa and measured as the percent break per hour, linearly decreased from 6.21% measured in the control, to 0.98% when the concentration of the feed additive of the invention reached 50 grams / liter. As can be seen in this dramatic and significant response to the feed additive of the invention, the predation of protozoa of the bacterium, S. ruminantium, which is very widely known to the practitioners of the art, will be a direct function of population concentrations. of ruminal protozoa was reduced to levels less than 1% per hour when the feed additive of the invention was incorporated into the culture medium. Example 2 These experiments used the method of Waliace et al. And measured the anti-protozoal activity of: (a) a combination of 60/40 fermentable soluble fermentations of liquid glutamic acid and dried fermentation solids of corn dried in a carrier of intermediate products of wheat (50/50 weight / weight bases), using the forced air method as described above, but then formed in an aqueous solution to give an equivalent concentration of 10 grams of dry material per liter; (b) glutamic acid fermentation soluble and corn fermentation soluble in liquid form alone; (c) the preparation as in (a) but in autoclave; (d) the preparation as in (a) but in ashes; (e) the preparation as in (a) but undergoing dialysis for 24 hours.
The results of these experiments are summarized in Tables 2 and 3. As can be seen in these data, the anti-protozoal activity of the feed additive of the invention is similar to that reported for a comparable dilution in Table 1, with the Except for the samples in ashes and autoclaved, where the biological activity of the invention are destroyed, as indicated by the lack of a significant response on the control. In the dialyzed sample, the anti-protozoal activity of the invention was not affected, indicating that the biological agent is insoluble in water or has a molecular weight greater than 10,000. Finally, the anti-protozoal activity of the food additive of the invention is similar regardless of whether the source is a mixture of the fermentation solubles or this alone. An additional experiment that uses the same methods but that measures the anti-protozoal activity of the cell bodies of C. glutamicum isolated from fermentations of glutamic acid, demonstrated anti-protozoal activity comparable to that of dry glutamic fermentation soluble solos alone, thus indicating that the active agent is more likely a component of the cellular cream itself, since no anti-protozoal activity was found in the vehicle of intermediate products of wheat. The Effects of Feeding the Food Additive of the Invention on Numbers of Rumen Protozoa in Inflorable Breeding Cattle Soybean meal was used as the main source of protein in a control diet (C) for lactating dairy cows. In the experimental diet, 100% of the soybean meal (4.32 kg) was replaced with a comparable amount of the feed additive of the invention, in this case, a 60/40 weight / weight mixture of glutamic acid fermentation soluble and corn fermentation solubles dried on a wheat intermediate product vehicle using the forced air method as previously described. Two lactating dairy cows, conditioned with a canine in the rumen, were fed the diet (C) for a period of two weeks, after which the experimental diet was changed. During the second week in each diet, the contents of the rumen were removed from each cow and the numbers of protozoa were determined according to the procedure of Abe and Kumeno (J. Animal Sc. 36: 941, 1973). The results of this experiment are summarized in Table 3. As can be seen, the invention of the feed additive reduced numbers of protozoa by an average of 66% over those observed when the animals consumed the control diet. These data confirm the in vitro observations previously reported and verify the value of the feed additive under field conditions to control the numbers of protozoa in vivo.
Table 1. The Effect of Feed Additive on Protozoan Activity as Determined by its Effect on Rupture of Seleneomonas ruminantium in Rumen Fluid
Concentration of Feed Additive Rupture of S. ruminantium
(gr / i) (% / hr) Control (without Feed Additive present) 6.21 1 gr / l 5.24 10 gr / l 3.70 25 gr / l 1.54 50 gr / l 0.98
Table 2. The Effects of Processing on the Efficacy of the Feed Additive After Dilution at 10 gr / l
Physical Form Rupture of S. ruminantium (%) hr) Control (without feed additive present) 6.86 Diluted at 10 gr / l 3.30 Diluted and treated with Autoclave 6.02 Diluted and made ashes 5.60 Diluted and Dialyzed 3.61
Table 3. A Comparison of Feeding Additive as Prepared from Soluble Solubles of Liquid Acid or Solubles of Fermentation of Corn against a Control and Intermediate Products of Wheat against Protozoa Activity as Determined by its Effect on Rupture of Selenomonas ruminantium in Rumen Fluid.
Source Rupture of S. ruminantium (% / hr) Control 7.02 Wheat intermediate products 7.06 Solubles of glutamic acid (100 ml / l) 4.59 Solubles of fermentation of corn (100 ml / l) 3.58
Table 4. The Effect on Feeding Additive on Protozoan Numbers When Used as a Dietary Component in Dairy Lactating Cattle. Protozoan Total Cow No. / ml of Rumen Content Feed Additive Control
1 6.8 X 105 2.9 X 10s 2 7.4 X 105 1.8 X 105 Average 7.1 X 105 2.4 X 105
The average decrease in numbers of protozoa due to the presence of the feed additive was 66%. As used herein, the control, reduction, and prevention of "anti-protozoal amounts" of the composition or compounds of the invention, are those amounts that decrease the numbers of protozoa present in the rumen, which prevent further growth and that prevent growth where protozoa are not present, respectively.
Claims (6)
- CLAIMS 1. A method for controlling, reducing or preventing the growth of protozoan bacteria in the rumen of a ruminant comprising feeding a ruminant an anti-protozoan amount of a composition comprising Corynebacterium cell bodies, Brevibacterium cell bodies, fermentation solubles of glutamic acid, soluble fermentation of corn, or a mixture of any two or more of the cell bodies of Corynebacterium, cell bodies of Brevibacterium, soluble fermentation of glutamic acid and soluble fermentation of corn.
- 2. The method of claim 1, wherein the composition further comprises a vehicle.
- 3. The method of claim 2, wherein said vehicle is an intermediate product of wheat.
- 4. The method of claim 1, wherein the composition further comprises glutamic acid. The method of claim 1, wherein the composition further comprises a proteolytic, amylolytic and cellulitic enzyme of bacterial or fungal origin. The method of claim 1, wherein the cell and soluble bodies have been dried at a total moisture content of less than 30% by weight at a temperature of not less than about 26.6 ° C and not more than about 482.2 ° C.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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US08/688,955 | 1996-07-31 |
Publications (1)
Publication Number | Publication Date |
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MXPA99001131A true MXPA99001131A (en) | 2000-05-01 |
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