MXPA05004016A - Clonacion de genes de citocromo p450 de nicotiana. - Google Patents

Clonacion de genes de citocromo p450 de nicotiana.

Info

Publication number
MXPA05004016A
MXPA05004016A MXPA05004016A MXPA05004016A MXPA05004016A MX PA05004016 A MXPA05004016 A MX PA05004016A MX PA05004016 A MXPA05004016 A MX PA05004016A MX PA05004016 A MXPA05004016 A MX PA05004016A MX PA05004016 A MXPA05004016 A MX PA05004016A
Authority
MX
Mexico
Prior art keywords
nicotiana
cytochrome
cloning
genes
enzymes
Prior art date
Application number
MXPA05004016A
Other languages
English (en)
Inventor
Dongmei Xu
Original Assignee
Us Smokeless Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Smokeless Tobacco Co filed Critical Us Smokeless Tobacco Co
Publication of MXPA05004016A publication Critical patent/MXPA05004016A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invencion se refiere a enzimas p450 y secuencias de acido nucleico que codifican enzimas p450 en Nicotiana, y metodos de usar esas enzimas y secuencias de acido nucleico para alterar fenotipos de plantas.
MXPA05004016A 2002-10-16 2003-10-16 Clonacion de genes de citocromo p450 de nicotiana. MXPA05004016A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41893302P 2002-10-16 2002-10-16
US48536803P 2003-07-08 2003-07-08
US50398903P 2003-09-18 2003-09-18
PCT/US2003/032722 WO2004035745A2 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
MXPA05004016A true MXPA05004016A (es) 2006-01-27

Family

ID=32110829

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05004016A MXPA05004016A (es) 2002-10-16 2003-10-16 Clonacion de genes de citocromo p450 de nicotiana.

Country Status (9)

Country Link
EP (3) EP2336305A3 (es)
JP (2) JP2006519590A (es)
KR (1) KR20050052541A (es)
AP (1) AP2005003286A0 (es)
AU (1) AU2003301431A1 (es)
BR (1) BR0315388A (es)
CA (1) CA2501631A1 (es)
MX (1) MXPA05004016A (es)
WO (1) WO2004035745A2 (es)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
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US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700851B2 (en) * 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
AP2004003122A0 (en) * 2002-03-12 2004-09-30 Us Smokeless Tobacco Co Cloning of cytochrome P450 genes from nicotiana
EP1694832B1 (en) * 2003-10-16 2012-04-18 U.S. Smokeless Tobacco Company LLC Use of a cytochrome p450 gene from nicotiana
ZA200602938B (en) 2003-10-16 2008-06-25 Us Smokeless Tobacco Company Cloning of cytochrome p450 genes from Nicotiana
ES2384243T3 (es) * 2003-10-16 2012-07-02 U.S. Smokeless Tobacco Company Uso de un gen para el citocromo P450 de nicotiana
PL1675957T3 (pl) * 2003-10-16 2012-06-29 U S Smokeless Tobacco Company Llc Klonowanie genów cytochromu p450 z Nicotiana
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
CN101384710B (zh) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
JP4753937B2 (ja) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Ｎｉｃｏｔｉａｎａ核酸分子およびその用途
ES2535643T3 (es) * 2004-04-29 2015-05-13 U.S. Smokeless Tobacco Company Llc Moléculas de ácido nucleico de nicotiana y usos de las mismas
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
PL2295549T3 (pl) 2004-04-29 2015-10-30 U S Smokeless Tobacco Company Llc Cząsteczki kwasu nukleinowego Nicotiana i ich zastosowanie
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US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
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CA2666383C (en) * 2006-10-13 2015-12-22 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
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EP2220231B1 (en) 2007-11-12 2017-10-11 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
CA2786813C (en) 2010-01-15 2023-03-28 North Carolina State University Compositions and methods for minimizing nornicotine synthesis in tobacco
WO2012118754A1 (en) 2011-02-28 2012-09-07 Altria Client Services Inc. Tobacco inbred plants albex1f and albex1ms
EP2680689A1 (en) 2011-02-28 2014-01-08 North Carolina State University Tobacco inbred plants ncbex1f, ncbex1ms, and nc ex90
CN105517430A (zh) 2013-01-11 2016-04-20 北卡罗莱纳州立大学 烟草近交植物k326 src、cms k326 src、k346 src、cms k346 src、nc1562-1 src、nctg-61 src、cms nctg-61 src和杂交种nc196 src
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WO2015134438A1 (en) 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US9596823B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof

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Also Published As

Publication number Publication date
EP2336305A2 (en) 2011-06-22
EP2336305A3 (en) 2011-10-05
EP2204444A1 (en) 2010-07-07
JP2010142241A (ja) 2010-07-01
EP1581633A2 (en) 2005-10-05
AU2003301431A1 (en) 2004-05-04
CA2501631A1 (en) 2004-04-29
WO2004035745A8 (en) 2007-12-06
AU2003301431A8 (en) 2008-02-28
AP2005003286A0 (en) 2005-06-30
EP1581633A4 (en) 2008-10-15
KR20050052541A (ko) 2005-06-02
JP2006519590A (ja) 2006-08-31
WO2004035745A2 (en) 2004-04-29
BR0315388A (pt) 2005-08-23

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