MX9306676A - SOMATOTROPINE MODIFICATIONS. - Google Patents
SOMATOTROPINE MODIFICATIONS.Info
- Publication number
- MX9306676A MX9306676A MX9306676A MX9306676A MX9306676A MX 9306676 A MX9306676 A MX 9306676A MX 9306676 A MX9306676 A MX 9306676A MX 9306676 A MX9306676 A MX 9306676A MX 9306676 A MX9306676 A MX 9306676A
- Authority
- MX
- Mexico
- Prior art keywords
- somatotropins
- modifications
- histidine
- glutamic acid
- acid replaces
- Prior art date
Links
- 238000012986 modification Methods 0.000 title abstract 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract 2
- 235000013922 glutamic acid Nutrition 0.000 abstract 2
- 239000004220 glutamic acid Substances 0.000 abstract 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 239000004475 Arginine Substances 0.000 abstract 1
- 241000283690 Bos taurus Species 0.000 abstract 1
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 abstract 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical group OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 abstract 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 241000282887 Suidae Species 0.000 abstract 1
- 210000000577 adipose tissue Anatomy 0.000 abstract 1
- 230000004075 alteration Effects 0.000 abstract 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
- 230000004071 biological effect Effects 0.000 abstract 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 abstract 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 abstract 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 abstract 1
- 239000008267 milk Substances 0.000 abstract 1
- 210000004080 milk Anatomy 0.000 abstract 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 abstract 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N phenylalanine group Chemical group N[C@@H](CC1=CC=CC=C1)C(=O)O COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000002741 site-directed mutagenesis Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Reproductive Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pregnancy & Childbirth (AREA)
- Gynecology & Obstetrics (AREA)
- Peptides Or Proteins (AREA)
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se describen modificaciones a la somatotropina que muestran una mejora inesperada en la estabilidad conformacional y química sin pérdida de actividad biológica. Las alteraciones de estas características se obtienen mediante la modificación de las somatotropinas por mutagénesis dirigidas al sitio. Estos cambios aumentan la estabilidad de almacenamiento de estas proteínas. Ejemplos de modificaciones incluyen las somatotropinas porcinas en las que la histidina y la arginina substituyen a la glutamina y la histidina en las posiciones 19 y 20, respectivamente (Q19h,h2or), el ácido glutámico substituye a la fenilalanina en la posición 52 (F52E) y el ácido glutámico substituye a la leucina en la posición 137 (I137E). Las somatotropinas modificadas son útiles para aumentar las características de crecimiento en especies animales, por ej., para aumentar la relación de tejido magro a tejido adiposo en cerdos y la producción de leche en las vacas.Modifications to somatotropin are described that show an unexpected improvement in conformational and chemical stability without loss of biological activity. Alterations of these characteristics are obtained by modifying somatotropins by site-directed mutagenesis. These changes increase the storage stability of these proteins. Examples of modifications include porcine somatotropins in which histidine and arginine replace glutamine and histidine at positions 19 and 20, respectively (Q19h, h2or), glutamic acid replaces phenylalanine at position 52 (F52E) and glutamic acid replaces leucine at position 137 (I137E). Modified somatotropins are useful for increasing growth characteristics in animal species, eg, for increasing the ratio of lean to adipose tissue in pigs and milk production in cows.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97109892A | 1992-10-28 | 1992-10-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX9306676A true MX9306676A (en) | 1994-04-29 |
Family
ID=25517926
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX9306676A MX9306676A (en) | 1992-10-28 | 1993-10-27 | SOMATOTROPINE MODIFICATIONS. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0669939A1 (en) |
| JP (1) | JPH08502963A (en) |
| AU (1) | AU5142293A (en) |
| CA (1) | CA2145546A1 (en) |
| MX (1) | MX9306676A (en) |
| WO (1) | WO1994010200A1 (en) |
| ZA (1) | ZA936811B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9723955D0 (en) | 1997-11-14 | 1998-01-07 | Generic Biolog Limited | Improvements in or relating to detection of molecules in samples |
| GB2375765B (en) * | 1997-11-14 | 2003-02-26 | Kymed Gb Ltd | Tagged growth hormone molecules |
| US6844322B2 (en) | 2000-07-21 | 2005-01-18 | Monsanto Technology Llc | Method for induced lactation |
| KR20110039348A (en) | 2008-08-06 | 2011-04-15 | 노보 노르디스크 헬스 케어 악티엔게젤샤프트 | Conjugated proteins with prolonged in vivo efficacy |
| TWI504405B (en) | 2009-01-22 | 2015-10-21 | Novo Nordisk Healthcare Ag | Stable growth hormone compounds |
| EP2461831B1 (en) | 2009-08-06 | 2018-11-21 | Novo Nordisk Health Care AG | Growth hormones with prolonged in-vivo efficacy |
| WO2011089255A1 (en) | 2010-01-22 | 2011-07-28 | Novo Nordisk Health Care Ag | Growth hormones with prolonged in-vivo efficacy |
| RU2012134974A (en) | 2010-01-22 | 2014-02-27 | Ново Нордиск Хелс Кеа Аг | STABILIZED GROWTH HORMONE COMPOUND |
| EP2718314A2 (en) * | 2011-06-10 | 2014-04-16 | Novo Nordisk A/S | Polypeptides |
| EP2981282B1 (en) | 2013-04-05 | 2020-11-04 | Novo Nordisk Health Care AG | Growth hormone compound formulation |
| WO2015070911A1 (en) * | 2013-11-14 | 2015-05-21 | Lysando Ag | Modified kz144 endolysin sequence |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE68929273T2 (en) * | 1988-08-24 | 2001-07-05 | American Cyanamid Co | Stabilization of somatotropins through modification of cysteine residues through site-specific mutagenesis or chemical derivatization |
| EP0454730A1 (en) * | 1989-01-19 | 1991-11-06 | The Upjohn Company | Somatotropin analogs |
| JPH04506800A (en) * | 1989-07-10 | 1992-11-26 | ジ・アップジョン・カンパニー | somatotropin analogs |
| US5310882A (en) * | 1990-11-30 | 1994-05-10 | American Cyanamid Company | Somatotropins with alterations in the α-helix 3 region |
-
1993
- 1993-09-15 ZA ZA936811A patent/ZA936811B/en unknown
- 1993-10-04 CA CA002145546A patent/CA2145546A1/en not_active Abandoned
- 1993-10-04 WO PCT/US1993/009232 patent/WO1994010200A1/en not_active Application Discontinuation
- 1993-10-04 AU AU51422/93A patent/AU5142293A/en not_active Abandoned
- 1993-10-04 JP JP6511061A patent/JPH08502963A/en active Pending
- 1993-10-04 EP EP93922424A patent/EP0669939A1/en not_active Ceased
- 1993-10-27 MX MX9306676A patent/MX9306676A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ZA936811B (en) | 1995-03-15 |
| JPH08502963A (en) | 1996-04-02 |
| CA2145546A1 (en) | 1994-05-11 |
| WO1994010200A1 (en) | 1994-05-11 |
| EP0669939A1 (en) | 1995-09-06 |
| AU5142293A (en) | 1994-05-24 |
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