MX371379B - Deteccion de la actividad de corte de una enzima. - Google Patents

Deteccion de la actividad de corte de una enzima.

Info

Publication number
MX371379B
MX371379B MX2016005313A MX2016005313A MX371379B MX 371379 B MX371379 B MX 371379B MX 2016005313 A MX2016005313 A MX 2016005313A MX 2016005313 A MX2016005313 A MX 2016005313A MX 371379 B MX371379 B MX 371379B
Authority
MX
Mexico
Prior art keywords
enzyme
cleavage activity
detection
activity
cleavage
Prior art date
Application number
MX2016005313A
Other languages
English (en)
Other versions
MX2016005313A (es
Inventor
Davis Paul
Parekh Gita
SCHOUTEN James
Original Assignee
Mologic Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mologic Ltd filed Critical Mologic Ltd
Publication of MX2016005313A publication Critical patent/MX2016005313A/es
Publication of MX371379B publication Critical patent/MX371379B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/12—Pulmonary diseases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/12—Pulmonary diseases
    • G01N2800/122—Chronic or obstructive airway disorders, e.g. asthma COPD
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/38—Pediatrics
    • G01N2800/382—Cystic fibrosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a detectar la actividad de corte de una enzima. Los diversos aspectos de la invención incluyen un dispositivo de detección de enzima, kit, método y uso para detectar o medir la presencia en una muestra de prueba de la actividad de una enzima capaz de cortar un substrato. La invención también se refiere a moléculas indicadoras y de unión útiles para llevar a cabo la invención. El substrato de la enzima contiene un sitio de unión escondido el cual solamente es revelado después del corte por la enzima.
MX2016005313A 2013-10-23 2014-10-23 Deteccion de la actividad de corte de una enzima. MX371379B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201318728A GB201318728D0 (en) 2013-10-23 2013-10-23 Detection of cleavage activity of an enzyme
PCT/GB2014/053171 WO2015059487A2 (en) 2013-10-23 2014-10-23 Detection of cleavage activity of an enzyme

Publications (2)

Publication Number Publication Date
MX2016005313A MX2016005313A (es) 2017-03-01
MX371379B true MX371379B (es) 2020-01-28

Family

ID=49727217

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016005313A MX371379B (es) 2013-10-23 2014-10-23 Deteccion de la actividad de corte de una enzima.

Country Status (11)

Country Link
US (2) US10866236B2 (es)
EP (1) EP3060915B1 (es)
JP (1) JP6496322B2 (es)
CN (1) CN105940304B (es)
AU (1) AU2014338709B2 (es)
BR (1) BR112016008956A2 (es)
CA (1) CA2928308C (es)
GB (1) GB201318728D0 (es)
MX (1) MX371379B (es)
RU (1) RU2016119422A (es)
WO (1) WO2015059487A2 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201605110D0 (en) 2016-03-24 2016-05-11 Mologic Ltd Detecting sepsis
EP3440113A1 (en) * 2016-04-08 2019-02-13 Gilead Sciences, Inc. Compositions and methods for treating cancer, inflammatory diseases and autoimmune diseases
US20190360019A1 (en) * 2016-11-16 2019-11-28 Universal Biosensors Pty Ltd Cleavage event transduction methods and products
ES2931536T3 (es) * 2017-08-31 2022-12-30 Somaprobes Sl Ensayo de flujo lateral para detectar la presencia de una célula de mamífero o bacterias específicas en una muestra biológica
AU2018346530A1 (en) 2017-10-04 2020-04-30 Massachusetts Institute Of Technology CRISPR effector system based diagnostics
US11732009B2 (en) * 2018-06-08 2023-08-22 Glympse Bio, Inc. Activity sensor with tunable analyte
GB201812331D0 (en) * 2018-07-27 2018-09-12 Mologic Ltd Bacterial vaginosis diagnostic

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4795702A (en) 1986-02-05 1989-01-03 Immunogon Associates Diagnostic method for gonorrhea by assay of IgA1 fragments
US7432061B2 (en) * 2002-05-31 2008-10-07 Biozyme, Inc. Assays for measuring matrix metalloproteinase activities
EP1452868A2 (en) 2003-02-27 2004-09-01 Pepscan Systems B.V. Method for selecting a candidate drug compound
WO2004099784A2 (en) * 2003-05-06 2004-11-18 Anamar Medical Ab Method for determining a tissue degradation process by detection of fibromodulin neoepitopes
GB0311961D0 (en) * 2003-05-23 2003-06-25 Health Prot Agency Mass-based toxin assay and substrates therefor
US20050079566A1 (en) * 2003-09-10 2005-04-14 Caterer Nigel Robert Analytical methods for determination of proteolytic cleavage at specific sites
CN1989411B (zh) * 2004-06-21 2012-06-27 普罗吉安拉公司 与蛋白水解活性相关的诊断和筛选方法以及试剂盒
EP1889919A4 (en) 2005-05-20 2009-04-15 Mitsubishi Kagaku Iatron Inc METHOD FOR ANALYZING AN ENZYME
GB2435511A (en) * 2006-02-23 2007-08-29 Mologic Ltd Protease detection
GB2437311A (en) 2006-04-07 2007-10-24 Mologic Ltd A protease detection product
US7897360B2 (en) 2006-12-15 2011-03-01 Kimberly-Clark Worldwide, Inc. Enzyme detection techniques
CA2864868A1 (en) * 2011-02-25 2012-11-15 Wellstat Diagnostics, Llc Assays for detecting enzymatic activity
WO2013116791A1 (en) * 2012-02-02 2013-08-08 Mosaic Biosciences, Inc. Biomaterials for delivery of blood extracts and methods of using same
GB201206976D0 (en) 2012-04-20 2012-06-06 Mologic Ltd An enzyme detection device

Also Published As

Publication number Publication date
BR112016008956A2 (pt) 2017-09-19
CN105940304A (zh) 2016-09-14
MX2016005313A (es) 2017-03-01
WO2015059487A2 (en) 2015-04-30
EP3060915B1 (en) 2018-12-05
AU2014338709A1 (en) 2016-06-02
JP6496322B2 (ja) 2019-04-03
WO2015059487A3 (en) 2015-09-17
US20200378963A1 (en) 2020-12-03
CA2928308A1 (en) 2015-04-30
GB201318728D0 (en) 2013-12-04
AU2014338709B2 (en) 2020-06-25
CN105940304B (zh) 2019-09-13
US20160238596A1 (en) 2016-08-18
EP3060915A2 (en) 2016-08-31
RU2016119422A (ru) 2017-11-28
US10866236B2 (en) 2020-12-15
JP2016535282A (ja) 2016-11-10
CA2928308C (en) 2023-07-11

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